pgsk3 beta s9  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc pgsk3 beta s9
    Pgsk3 Beta S9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pgsk3 β s9  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc pgsk3 β s9
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    anti rabbit pgsk3 β s9  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc anti rabbit pgsk3 β s9
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    pgsk3 β s9  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc pgsk3 β s9
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    anti pgsk3 β s9  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    pgsk3 s9 21 antibodies  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    pgsk3 β s9  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc pgsk3 β s9
    Repetitive treatments of the 5HT1A antagonist WAY results in reduced DNA synthesis and down-regulation of cell cycle and metabolic genes. ( a , b ) Representative flow cytometric analysis plots for BrdU+ cells at 48 and 72 h respectively. Bjab cells were exposed to indicated treatments of WAY ( a ) 1× (50 µM) or 2× (50 µM/24 h) for 48 h or ( b ) 1× (50 µM) or 3× (50 µM/24 h) for 72 h. In the last 4 h of incubation, cells were pulsed with BrdU (10 µM) and subjected to flow cytometric analysis. Data is presented as the mean of 3 independent experiments ± SD. Statistical comparison of control and treated cells is indicated ( * P < 0.05; *** P < 0.001; Two-way ANOVA test). ( c ) Bjab cells were exposed to repetitive treatments (WAY-3×; 50 µM/24 h) for 72 h and protein expression of pAKT, Total AKT, <t>pGSK3-β,</t> Total GSK3-β, cMYC and p53 were detected by Western blot using Beta-actin or Cyclophilin-A as loading controls. Bands were quantified using image analysis software from Li-cor and illustrated in the bar diagram. Data is presented as the mean of 4 independent experiments ± SD. Statistical comparison of control and treated cells is indicated (ns: non-significant; * P < 0.05; ** P < 0.01; *** P < 0.001; Two-way ANOVA test). The blot images shown in the figure were cropped from same part of the gel with an exposure time of 60 sec. Clear delineation with white line is used. Full-length blots and gels are presented in extended Supplementary Figure 5. ( d ) rtqPCR analysis of cMYC, p53, GSK3-β and AKT1 expression in Bjab cells exposed to repetitive treatments (WAY-3×; 50 µM/24 h) for 72 h. Expression levels were normalized to 18 s rRNA, calculated by 2(−ΔΔct) method and presented as a fold change compared to control cells. Data is presented as the mean of 3–4 independent experiments ± SD ( *** P < 0.001; Two-way ANOVA test). ( e ) rtqPCR analysis for 18 s rRNA, expression in Bjab cells exposed to repetitive treatments (WAY-3×; 50 µM/24 h) and control cells at 72 h (n = 6). Statistical comparison of control and treated cells is indicated (ns: non-significant; Mann-Whitney test).
    Pgsk3 β S9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Growth-inhibition of cell lines derived from B cell lymphomas through antagonism of serotonin receptor signaling"

    Article Title: Growth-inhibition of cell lines derived from B cell lymphomas through antagonism of serotonin receptor signaling

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-40825-x

    Repetitive treatments of the 5HT1A antagonist WAY results in reduced DNA synthesis and down-regulation of cell cycle and metabolic genes. ( a , b ) Representative flow cytometric analysis plots for BrdU+ cells at 48 and 72 h respectively. Bjab cells were exposed to indicated treatments of WAY ( a ) 1× (50 µM) or 2× (50 µM/24 h) for 48 h or ( b ) 1× (50 µM) or 3× (50 µM/24 h) for 72 h. In the last 4 h of incubation, cells were pulsed with BrdU (10 µM) and subjected to flow cytometric analysis. Data is presented as the mean of 3 independent experiments ± SD. Statistical comparison of control and treated cells is indicated ( * P < 0.05; *** P < 0.001; Two-way ANOVA test). ( c ) Bjab cells were exposed to repetitive treatments (WAY-3×; 50 µM/24 h) for 72 h and protein expression of pAKT, Total AKT, pGSK3-β, Total GSK3-β, cMYC and p53 were detected by Western blot using Beta-actin or Cyclophilin-A as loading controls. Bands were quantified using image analysis software from Li-cor and illustrated in the bar diagram. Data is presented as the mean of 4 independent experiments ± SD. Statistical comparison of control and treated cells is indicated (ns: non-significant; * P < 0.05; ** P < 0.01; *** P < 0.001; Two-way ANOVA test). The blot images shown in the figure were cropped from same part of the gel with an exposure time of 60 sec. Clear delineation with white line is used. Full-length blots and gels are presented in extended Supplementary Figure 5. ( d ) rtqPCR analysis of cMYC, p53, GSK3-β and AKT1 expression in Bjab cells exposed to repetitive treatments (WAY-3×; 50 µM/24 h) for 72 h. Expression levels were normalized to 18 s rRNA, calculated by 2(−ΔΔct) method and presented as a fold change compared to control cells. Data is presented as the mean of 3–4 independent experiments ± SD ( *** P < 0.001; Two-way ANOVA test). ( e ) rtqPCR analysis for 18 s rRNA, expression in Bjab cells exposed to repetitive treatments (WAY-3×; 50 µM/24 h) and control cells at 72 h (n = 6). Statistical comparison of control and treated cells is indicated (ns: non-significant; Mann-Whitney test).
    Figure Legend Snippet: Repetitive treatments of the 5HT1A antagonist WAY results in reduced DNA synthesis and down-regulation of cell cycle and metabolic genes. ( a , b ) Representative flow cytometric analysis plots for BrdU+ cells at 48 and 72 h respectively. Bjab cells were exposed to indicated treatments of WAY ( a ) 1× (50 µM) or 2× (50 µM/24 h) for 48 h or ( b ) 1× (50 µM) or 3× (50 µM/24 h) for 72 h. In the last 4 h of incubation, cells were pulsed with BrdU (10 µM) and subjected to flow cytometric analysis. Data is presented as the mean of 3 independent experiments ± SD. Statistical comparison of control and treated cells is indicated ( * P < 0.05; *** P < 0.001; Two-way ANOVA test). ( c ) Bjab cells were exposed to repetitive treatments (WAY-3×; 50 µM/24 h) for 72 h and protein expression of pAKT, Total AKT, pGSK3-β, Total GSK3-β, cMYC and p53 were detected by Western blot using Beta-actin or Cyclophilin-A as loading controls. Bands were quantified using image analysis software from Li-cor and illustrated in the bar diagram. Data is presented as the mean of 4 independent experiments ± SD. Statistical comparison of control and treated cells is indicated (ns: non-significant; * P < 0.05; ** P < 0.01; *** P < 0.001; Two-way ANOVA test). The blot images shown in the figure were cropped from same part of the gel with an exposure time of 60 sec. Clear delineation with white line is used. Full-length blots and gels are presented in extended Supplementary Figure 5. ( d ) rtqPCR analysis of cMYC, p53, GSK3-β and AKT1 expression in Bjab cells exposed to repetitive treatments (WAY-3×; 50 µM/24 h) for 72 h. Expression levels were normalized to 18 s rRNA, calculated by 2(−ΔΔct) method and presented as a fold change compared to control cells. Data is presented as the mean of 3–4 independent experiments ± SD ( *** P < 0.001; Two-way ANOVA test). ( e ) rtqPCR analysis for 18 s rRNA, expression in Bjab cells exposed to repetitive treatments (WAY-3×; 50 µM/24 h) and control cells at 72 h (n = 6). Statistical comparison of control and treated cells is indicated (ns: non-significant; Mann-Whitney test).

    Techniques Used: DNA Synthesis, Incubation, Expressing, Western Blot, Software, MANN-WHITNEY

    rabbit monoclonal anti pgsk3 s21 s9  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit monoclonal anti pgsk3 s21 s9
    KEY RESOURCES TABLE
    Rabbit Monoclonal Anti Pgsk3 S21 S9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Synthetic Lethality of Combined Bcl-2 Inhibition and p53 Activation in AML: Mechanisms and Superior Antileukemic Efficacy"

    Article Title: Synthetic Lethality of Combined Bcl-2 Inhibition and p53 Activation in AML: Mechanisms and Superior Antileukemic Efficacy

    Journal: Cancer cell

    doi: 10.1016/j.ccell.2017.11.003

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Imaging, Staining, Mutagenesis, Transfection, shRNA, Negative Control, Plasmid Preparation, Software, In Vivo Imaging, Microscopy, Flow Cytometry

    rabbit anti pgsk3 s9 21  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc rabbit anti pgsk3 s9 21
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    pgsk3 s9  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc pgsk3 s9
    Pgsk3 S9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    KEY RESOURCES TABLE

    Journal: Cancer cell

    Article Title: Synthetic Lethality of Combined Bcl-2 Inhibition and p53 Activation in AML: Mechanisms and Superior Antileukemic Efficacy

    doi: 10.1016/j.ccell.2017.11.003

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Rabbit monoclonal anti-pGSK3 (S21/S9) (clone D17D2) , Cell Signaling Technology , Cat#8566; RRID: AB_10860069.

    Techniques: Recombinant, Imaging, Staining, Mutagenesis, Transfection, shRNA, Negative Control, Plasmid Preparation, Software, In Vivo Imaging, Microscopy, Flow Cytometry