anti pan mglu5  (Alomone Labs)


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    Structured Review

    Alomone Labs anti pan mglu5
    Group 1 mGluRs interact with Actn4. A , schematic of wild type Actn4 and deletion mutants fused to GST used in a pulldown assay with mGlu 1b . B , the Actn4 CH region is necessary and sufficient for mGlu 1b binding. Shown are representative immunoblots ( IB )of a pulldown assay between GST-Actn4 fusion proteins from A and lysates of HEK293 cells transfected with mGlu 1b , probed with anti-mGlu 1b , and stained with Ponceau. C and D , the mGlu 1 cytoplasmic tail is sufficient for Actn4 binding. Shown is a schematic of chimeric constructs of VSVG protein fused to mGlu 1b or mGlu 1a carboxyl tails. Yellow highlights the membrane-proximal region of the tail that is conserved between receptor isoforms (CR region, see below). Also shown are representative immunoblots of input (20 μg) and pulldown (input lysate, 2 mg) between GST-Actn4 and VSVG-mGlu 1a-Tail ( D ) and VSVG-mGlu 1b-Tail ( C ) probed with anti-mGlu 1a or anti-mGlu 1b antibody, respectively. The estimated pulldown fraction in vitro is ∼1% of input receptors. E , Actn4 interacts directly with the CR region of the mGlu 1 intracellular tail. Shown is a schematic of the CR construct and immunoblots illustrating in vitro binding of a purified His-tagged CR fragment and GST-tagged full-length Actn4 ( GST-Actn4 , left panel ) or Actn4 CH region ( GST-Actn4 1–296 , right panel ). Shown are bound and unbound (14% of input GST-tagged proteins) fractions probed with anti-GST and anti-His tag antibodies. The arrow points to full-length GST-Actn4 ( left panel ). Lower molecular mass bands were derived from partial protein fragmentation. The estimated relative bound fractions are 40% and 26% of input for GST-Actn4 and GST-Actn41–296, respectively. F , Actn4 coprecipitates with mGlu 5 in the brain. Shown are representative immunoblots of input lysate and precipitated proteins (from 2.5 mg of input lysate) probed with anti-Actn4 (input, 10 μg) and anti-mGlu 5 (input, 40 μg). The estimated immunoprecipitation (IP) is 5% and 3% of input for <t>mGlu5</t> and Actn4, respectively.
    Anti Pan Mglu5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pan mglu5/product/Alomone Labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti pan mglu5 - by Bioz Stars, 2022-01
    85/100 stars

    Images

    1) Product Images from "Actinin-4 Governs Dendritic Spine Dynamics and Promotes Their Remodeling by Metabotropic Glutamate Receptors *"

    Article Title: Actinin-4 Governs Dendritic Spine Dynamics and Promotes Their Remodeling by Metabotropic Glutamate Receptors *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M115.640136

    Group 1 mGluRs interact with Actn4. A , schematic of wild type Actn4 and deletion mutants fused to GST used in a pulldown assay with mGlu 1b . B , the Actn4 CH region is necessary and sufficient for mGlu 1b binding. Shown are representative immunoblots ( IB )of a pulldown assay between GST-Actn4 fusion proteins from A and lysates of HEK293 cells transfected with mGlu 1b , probed with anti-mGlu 1b , and stained with Ponceau. C and D , the mGlu 1 cytoplasmic tail is sufficient for Actn4 binding. Shown is a schematic of chimeric constructs of VSVG protein fused to mGlu 1b or mGlu 1a carboxyl tails. Yellow highlights the membrane-proximal region of the tail that is conserved between receptor isoforms (CR region, see below). Also shown are representative immunoblots of input (20 μg) and pulldown (input lysate, 2 mg) between GST-Actn4 and VSVG-mGlu 1a-Tail ( D ) and VSVG-mGlu 1b-Tail ( C ) probed with anti-mGlu 1a or anti-mGlu 1b antibody, respectively. The estimated pulldown fraction in vitro is ∼1% of input receptors. E , Actn4 interacts directly with the CR region of the mGlu 1 intracellular tail. Shown is a schematic of the CR construct and immunoblots illustrating in vitro binding of a purified His-tagged CR fragment and GST-tagged full-length Actn4 ( GST-Actn4 , left panel ) or Actn4 CH region ( GST-Actn4 1–296 , right panel ). Shown are bound and unbound (14% of input GST-tagged proteins) fractions probed with anti-GST and anti-His tag antibodies. The arrow points to full-length GST-Actn4 ( left panel ). Lower molecular mass bands were derived from partial protein fragmentation. The estimated relative bound fractions are 40% and 26% of input for GST-Actn4 and GST-Actn41–296, respectively. F , Actn4 coprecipitates with mGlu 5 in the brain. Shown are representative immunoblots of input lysate and precipitated proteins (from 2.5 mg of input lysate) probed with anti-Actn4 (input, 10 μg) and anti-mGlu 5 (input, 40 μg). The estimated immunoprecipitation (IP) is 5% and 3% of input for mGlu5 and Actn4, respectively.
    Figure Legend Snippet: Group 1 mGluRs interact with Actn4. A , schematic of wild type Actn4 and deletion mutants fused to GST used in a pulldown assay with mGlu 1b . B , the Actn4 CH region is necessary and sufficient for mGlu 1b binding. Shown are representative immunoblots ( IB )of a pulldown assay between GST-Actn4 fusion proteins from A and lysates of HEK293 cells transfected with mGlu 1b , probed with anti-mGlu 1b , and stained with Ponceau. C and D , the mGlu 1 cytoplasmic tail is sufficient for Actn4 binding. Shown is a schematic of chimeric constructs of VSVG protein fused to mGlu 1b or mGlu 1a carboxyl tails. Yellow highlights the membrane-proximal region of the tail that is conserved between receptor isoforms (CR region, see below). Also shown are representative immunoblots of input (20 μg) and pulldown (input lysate, 2 mg) between GST-Actn4 and VSVG-mGlu 1a-Tail ( D ) and VSVG-mGlu 1b-Tail ( C ) probed with anti-mGlu 1a or anti-mGlu 1b antibody, respectively. The estimated pulldown fraction in vitro is ∼1% of input receptors. E , Actn4 interacts directly with the CR region of the mGlu 1 intracellular tail. Shown is a schematic of the CR construct and immunoblots illustrating in vitro binding of a purified His-tagged CR fragment and GST-tagged full-length Actn4 ( GST-Actn4 , left panel ) or Actn4 CH region ( GST-Actn4 1–296 , right panel ). Shown are bound and unbound (14% of input GST-tagged proteins) fractions probed with anti-GST and anti-His tag antibodies. The arrow points to full-length GST-Actn4 ( left panel ). Lower molecular mass bands were derived from partial protein fragmentation. The estimated relative bound fractions are 40% and 26% of input for GST-Actn4 and GST-Actn41–296, respectively. F , Actn4 coprecipitates with mGlu 5 in the brain. Shown are representative immunoblots of input lysate and precipitated proteins (from 2.5 mg of input lysate) probed with anti-Actn4 (input, 10 μg) and anti-mGlu 5 (input, 40 μg). The estimated immunoprecipitation (IP) is 5% and 3% of input for mGlu5 and Actn4, respectively.

    Techniques Used: Binding Assay, Western Blot, Transfection, Staining, Construct, In Vitro, Purification, Derivative Assay, Immunoprecipitation

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    Alomone Labs anti pan mglu5
    Group 1 mGluRs interact with Actn4. A , schematic of wild type Actn4 and deletion mutants fused to GST used in a pulldown assay with mGlu 1b . B , the Actn4 CH region is necessary and sufficient for mGlu 1b binding. Shown are representative immunoblots ( IB )of a pulldown assay between GST-Actn4 fusion proteins from A and lysates of HEK293 cells transfected with mGlu 1b , probed with anti-mGlu 1b , and stained with Ponceau. C and D , the mGlu 1 cytoplasmic tail is sufficient for Actn4 binding. Shown is a schematic of chimeric constructs of VSVG protein fused to mGlu 1b or mGlu 1a carboxyl tails. Yellow highlights the membrane-proximal region of the tail that is conserved between receptor isoforms (CR region, see below). Also shown are representative immunoblots of input (20 μg) and pulldown (input lysate, 2 mg) between GST-Actn4 and VSVG-mGlu 1a-Tail ( D ) and VSVG-mGlu 1b-Tail ( C ) probed with anti-mGlu 1a or anti-mGlu 1b antibody, respectively. The estimated pulldown fraction in vitro is ∼1% of input receptors. E , Actn4 interacts directly with the CR region of the mGlu 1 intracellular tail. Shown is a schematic of the CR construct and immunoblots illustrating in vitro binding of a purified His-tagged CR fragment and GST-tagged full-length Actn4 ( GST-Actn4 , left panel ) or Actn4 CH region ( GST-Actn4 1–296 , right panel ). Shown are bound and unbound (14% of input GST-tagged proteins) fractions probed with anti-GST and anti-His tag antibodies. The arrow points to full-length GST-Actn4 ( left panel ). Lower molecular mass bands were derived from partial protein fragmentation. The estimated relative bound fractions are 40% and 26% of input for GST-Actn4 and GST-Actn41–296, respectively. F , Actn4 coprecipitates with mGlu 5 in the brain. Shown are representative immunoblots of input lysate and precipitated proteins (from 2.5 mg of input lysate) probed with anti-Actn4 (input, 10 μg) and anti-mGlu 5 (input, 40 μg). The estimated immunoprecipitation (IP) is 5% and 3% of input for <t>mGlu5</t> and Actn4, respectively.
    Anti Pan Mglu5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pan mglu5/product/Alomone Labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti pan mglu5 - by Bioz Stars, 2022-01
    85/100 stars
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    Group 1 mGluRs interact with Actn4. A , schematic of wild type Actn4 and deletion mutants fused to GST used in a pulldown assay with mGlu 1b . B , the Actn4 CH region is necessary and sufficient for mGlu 1b binding. Shown are representative immunoblots ( IB )of a pulldown assay between GST-Actn4 fusion proteins from A and lysates of HEK293 cells transfected with mGlu 1b , probed with anti-mGlu 1b , and stained with Ponceau. C and D , the mGlu 1 cytoplasmic tail is sufficient for Actn4 binding. Shown is a schematic of chimeric constructs of VSVG protein fused to mGlu 1b or mGlu 1a carboxyl tails. Yellow highlights the membrane-proximal region of the tail that is conserved between receptor isoforms (CR region, see below). Also shown are representative immunoblots of input (20 μg) and pulldown (input lysate, 2 mg) between GST-Actn4 and VSVG-mGlu 1a-Tail ( D ) and VSVG-mGlu 1b-Tail ( C ) probed with anti-mGlu 1a or anti-mGlu 1b antibody, respectively. The estimated pulldown fraction in vitro is ∼1% of input receptors. E , Actn4 interacts directly with the CR region of the mGlu 1 intracellular tail. Shown is a schematic of the CR construct and immunoblots illustrating in vitro binding of a purified His-tagged CR fragment and GST-tagged full-length Actn4 ( GST-Actn4 , left panel ) or Actn4 CH region ( GST-Actn4 1–296 , right panel ). Shown are bound and unbound (14% of input GST-tagged proteins) fractions probed with anti-GST and anti-His tag antibodies. The arrow points to full-length GST-Actn4 ( left panel ). Lower molecular mass bands were derived from partial protein fragmentation. The estimated relative bound fractions are 40% and 26% of input for GST-Actn4 and GST-Actn41–296, respectively. F , Actn4 coprecipitates with mGlu 5 in the brain. Shown are representative immunoblots of input lysate and precipitated proteins (from 2.5 mg of input lysate) probed with anti-Actn4 (input, 10 μg) and anti-mGlu 5 (input, 40 μg). The estimated immunoprecipitation (IP) is 5% and 3% of input for mGlu5 and Actn4, respectively.

    Journal: The Journal of Biological Chemistry

    Article Title: Actinin-4 Governs Dendritic Spine Dynamics and Promotes Their Remodeling by Metabotropic Glutamate Receptors *

    doi: 10.1074/jbc.M115.640136

    Figure Lengend Snippet: Group 1 mGluRs interact with Actn4. A , schematic of wild type Actn4 and deletion mutants fused to GST used in a pulldown assay with mGlu 1b . B , the Actn4 CH region is necessary and sufficient for mGlu 1b binding. Shown are representative immunoblots ( IB )of a pulldown assay between GST-Actn4 fusion proteins from A and lysates of HEK293 cells transfected with mGlu 1b , probed with anti-mGlu 1b , and stained with Ponceau. C and D , the mGlu 1 cytoplasmic tail is sufficient for Actn4 binding. Shown is a schematic of chimeric constructs of VSVG protein fused to mGlu 1b or mGlu 1a carboxyl tails. Yellow highlights the membrane-proximal region of the tail that is conserved between receptor isoforms (CR region, see below). Also shown are representative immunoblots of input (20 μg) and pulldown (input lysate, 2 mg) between GST-Actn4 and VSVG-mGlu 1a-Tail ( D ) and VSVG-mGlu 1b-Tail ( C ) probed with anti-mGlu 1a or anti-mGlu 1b antibody, respectively. The estimated pulldown fraction in vitro is ∼1% of input receptors. E , Actn4 interacts directly with the CR region of the mGlu 1 intracellular tail. Shown is a schematic of the CR construct and immunoblots illustrating in vitro binding of a purified His-tagged CR fragment and GST-tagged full-length Actn4 ( GST-Actn4 , left panel ) or Actn4 CH region ( GST-Actn4 1–296 , right panel ). Shown are bound and unbound (14% of input GST-tagged proteins) fractions probed with anti-GST and anti-His tag antibodies. The arrow points to full-length GST-Actn4 ( left panel ). Lower molecular mass bands were derived from partial protein fragmentation. The estimated relative bound fractions are 40% and 26% of input for GST-Actn4 and GST-Actn41–296, respectively. F , Actn4 coprecipitates with mGlu 5 in the brain. Shown are representative immunoblots of input lysate and precipitated proteins (from 2.5 mg of input lysate) probed with anti-Actn4 (input, 10 μg) and anti-mGlu 5 (input, 40 μg). The estimated immunoprecipitation (IP) is 5% and 3% of input for mGlu5 and Actn4, respectively.

    Article Snippet: The following antibodies were used: goat polyclonal anti-GAPDH (GenScript); chicken polyclonal anti-MAP2 (EnCor Biotech); the rabbit monoclonal antibodies anti-Actn4, anti-Actn1, and anti-mGlu5 (Epitomics); the rabbit polyclonal antibodies anti-GFP (Santa Cruz Biotechnology), anti-pan-mGlu1 , anti-pan-mGlu5 (Alomone Labs), anti-mGlu5 (GenScript), anti-PSD95 (Zymed Laboratories Inc.), anti-Actn4 (Enzo Life Sciences), anti-phospho-ERK1/2(Thr-202/Tyr-204), anti-ERK1/2, anti-phospho-CaMKII(Thr-286/Thr-287), and anti-CaMKII (Cell Signaling Technology); and the mouse monoclonal antibodies anti-MAP2 clone AP20 (Roche Applied Science), anti-actinin clone EA-53 (Sigma-Aldrich), anti-Actn4 (Abnova), anti-mGlu1a (BD Biosciences), anti-PSD95 clone K28/43 (University of California Davis/National Institutes of Health NeuroMab Facility), and anti-His tag (Aviva Systems Biology).

    Techniques: Binding Assay, Western Blot, Transfection, Staining, Construct, In Vitro, Purification, Derivative Assay, Immunoprecipitation