anti pakt s473 af488  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti pakt s473 af488

    Anti Pakt S473 Af488, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pakt s473 af488/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
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    anti pakt s473 af488 - by Bioz Stars, 2024-07
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    1) Product Images from "CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci"

    Article Title: CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci

    Journal: Cell Reports

    doi: 10.1016/j.celrep.2020.108545


    Figure Legend Snippet:

    Techniques Used: Purification, Recombinant, Software

    rabbit anti pakt s473 af488  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc rabbit anti pakt s473 af488

    Rabbit Anti Pakt S473 Af488, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti pakt s473 af488/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti pakt s473 af488 - by Bioz Stars, 2024-07
    94/100 stars

    Images

    1) Product Images from "CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci"

    Article Title: CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci

    Journal: Cell Reports

    doi: 10.1016/j.celrep.2020.108545


    Figure Legend Snippet:

    Techniques Used: Purification, Recombinant, Software

    anti pakt s473 af488  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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  • 94

    Structured Review

    Cell Signaling Technology Inc anti pakt s473 af488
    Anti Pakt S473 Af488, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pakt s473 af488/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti pakt s473 af488 - by Bioz Stars, 2024-07
    94/100 stars

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    Structured Review

    Becton Dickinson anti pakt s473 af488
    Akt regulates development of both nT H 17 and iT H 17 cells. (a) Akt phosphorylation (p- Akt) at the <t>S473</t> site was determined for the indicated wild-type (WT) thymocyte populations by phospho-flow staining. Naive CD4 SP (CD4 SP CD44 lo CCR6 − ), nT reg (CD4 SP Foxp3 + ) and nT H 17 cells (CD4 SP CD44 hi CCR6 + ) were analyzed. (b) S6K phosphorylation (p-S6K) and S6 phosphorylation (p-S6) was assessed in the indicated thymocyte populations from WT mice by flow cytometry. The specificity of the phospho-flow staining was verified by treatment of thymocytes with rapamycin for 1 h at 37 °C prior to staining. (c) IL-17 is expressed in thymocytes from human fetal thymi upon ex vivo stimulation for 5 h with PMA/ionomycin and brefeldin A. Flow plots are gated on live lymphocytes (left) and CD4 SP cells (middle and right). (d) Phospho-flow analysis of p-Akt at the S473 site is shown for the indicated thymocyte populations from human fetal thymi. (e) Expression of IL-17A, ROR-γt, CCR6, and Foxp3 is shown for thymocytes from day 7 of E15-initiated FTOC, cultured for the last 2 days in the presence of indicated concentrations of allosteric Akt inhibitor, AKTi. Cultures were stimulated with PMA/ionomycin prior to staining. Representative flow plots are gated on CD4 SP TCRβ + TCRγδ − cells. Graphs show either the percent of IL-17A + or Foxp3 + cells among CD4 SP cells pooled from three independent experiments ( n = 5 thymi per condition; mean ± SEM; * P <0.0001, ** P =0.0124; P value from two-tailed Student’s t - test). (f) IL-17 production in thymocytes from WT and Myr-Akt mice following ex vivo stimulation was determined. Representative flow plots show staining on CD4 SP TCRβ + TCRγδ − gated cells. Graphs are of pooled data from two independent experiments ( n ≥ 5; mean ± SEM; * P =0.013, P value from two-tailed Student’s t -test). (g) WT naïve (CD44 lo CD62L hi CD25 − ) CD4 + T cells were activated for 18 h with anti- CD3 plus anti-CD28, followed by 36 h of culture in iT H 17-polarizing conditions in the presence of indicated concentrations of AKTi. IL-17 or Foxp3 expression was determined following restimulation with PMA/ionomycin. Representative flow plots are shown. The graph is of pooled data from n = 3 per condition (bars and error bars represent mean ±SEM. * P ≤ 0.05, ** P ≤ 0.001 (one-way ANOVA followed by Dunnett’s post-test with 0μM as control group). Data are representative of at least three independent experiments ( a–d,g ).
    Anti Pakt S473 Af488, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pakt s473 af488/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti pakt s473 af488 - by Bioz Stars, 2024-07
    86/100 stars

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    1) Product Images from "Akt and mTOR pathways differentially regulate the development of natural and inducible T H 17 cells"

    Article Title: Akt and mTOR pathways differentially regulate the development of natural and inducible T H 17 cells

    Journal: Nature immunology

    doi: 10.1038/ni.2607

    Akt regulates development of both nT H 17 and iT H 17 cells. (a) Akt phosphorylation (p- Akt) at the S473 site was determined for the indicated wild-type (WT) thymocyte populations by phospho-flow staining. Naive CD4 SP (CD4 SP CD44 lo CCR6 − ), nT reg (CD4 SP Foxp3 + ) and nT H 17 cells (CD4 SP CD44 hi CCR6 + ) were analyzed. (b) S6K phosphorylation (p-S6K) and S6 phosphorylation (p-S6) was assessed in the indicated thymocyte populations from WT mice by flow cytometry. The specificity of the phospho-flow staining was verified by treatment of thymocytes with rapamycin for 1 h at 37 °C prior to staining. (c) IL-17 is expressed in thymocytes from human fetal thymi upon ex vivo stimulation for 5 h with PMA/ionomycin and brefeldin A. Flow plots are gated on live lymphocytes (left) and CD4 SP cells (middle and right). (d) Phospho-flow analysis of p-Akt at the S473 site is shown for the indicated thymocyte populations from human fetal thymi. (e) Expression of IL-17A, ROR-γt, CCR6, and Foxp3 is shown for thymocytes from day 7 of E15-initiated FTOC, cultured for the last 2 days in the presence of indicated concentrations of allosteric Akt inhibitor, AKTi. Cultures were stimulated with PMA/ionomycin prior to staining. Representative flow plots are gated on CD4 SP TCRβ + TCRγδ − cells. Graphs show either the percent of IL-17A + or Foxp3 + cells among CD4 SP cells pooled from three independent experiments ( n = 5 thymi per condition; mean ± SEM; * P <0.0001, ** P =0.0124; P value from two-tailed Student’s t - test). (f) IL-17 production in thymocytes from WT and Myr-Akt mice following ex vivo stimulation was determined. Representative flow plots show staining on CD4 SP TCRβ + TCRγδ − gated cells. Graphs are of pooled data from two independent experiments ( n ≥ 5; mean ± SEM; * P =0.013, P value from two-tailed Student’s t -test). (g) WT naïve (CD44 lo CD62L hi CD25 − ) CD4 + T cells were activated for 18 h with anti- CD3 plus anti-CD28, followed by 36 h of culture in iT H 17-polarizing conditions in the presence of indicated concentrations of AKTi. IL-17 or Foxp3 expression was determined following restimulation with PMA/ionomycin. Representative flow plots are shown. The graph is of pooled data from n = 3 per condition (bars and error bars represent mean ±SEM. * P ≤ 0.05, ** P ≤ 0.001 (one-way ANOVA followed by Dunnett’s post-test with 0μM as control group). Data are representative of at least three independent experiments ( a–d,g ).
    Figure Legend Snippet: Akt regulates development of both nT H 17 and iT H 17 cells. (a) Akt phosphorylation (p- Akt) at the S473 site was determined for the indicated wild-type (WT) thymocyte populations by phospho-flow staining. Naive CD4 SP (CD4 SP CD44 lo CCR6 − ), nT reg (CD4 SP Foxp3 + ) and nT H 17 cells (CD4 SP CD44 hi CCR6 + ) were analyzed. (b) S6K phosphorylation (p-S6K) and S6 phosphorylation (p-S6) was assessed in the indicated thymocyte populations from WT mice by flow cytometry. The specificity of the phospho-flow staining was verified by treatment of thymocytes with rapamycin for 1 h at 37 °C prior to staining. (c) IL-17 is expressed in thymocytes from human fetal thymi upon ex vivo stimulation for 5 h with PMA/ionomycin and brefeldin A. Flow plots are gated on live lymphocytes (left) and CD4 SP cells (middle and right). (d) Phospho-flow analysis of p-Akt at the S473 site is shown for the indicated thymocyte populations from human fetal thymi. (e) Expression of IL-17A, ROR-γt, CCR6, and Foxp3 is shown for thymocytes from day 7 of E15-initiated FTOC, cultured for the last 2 days in the presence of indicated concentrations of allosteric Akt inhibitor, AKTi. Cultures were stimulated with PMA/ionomycin prior to staining. Representative flow plots are gated on CD4 SP TCRβ + TCRγδ − cells. Graphs show either the percent of IL-17A + or Foxp3 + cells among CD4 SP cells pooled from three independent experiments ( n = 5 thymi per condition; mean ± SEM; * P <0.0001, ** P =0.0124; P value from two-tailed Student’s t - test). (f) IL-17 production in thymocytes from WT and Myr-Akt mice following ex vivo stimulation was determined. Representative flow plots show staining on CD4 SP TCRβ + TCRγδ − gated cells. Graphs are of pooled data from two independent experiments ( n ≥ 5; mean ± SEM; * P =0.013, P value from two-tailed Student’s t -test). (g) WT naïve (CD44 lo CD62L hi CD25 − ) CD4 + T cells were activated for 18 h with anti- CD3 plus anti-CD28, followed by 36 h of culture in iT H 17-polarizing conditions in the presence of indicated concentrations of AKTi. IL-17 or Foxp3 expression was determined following restimulation with PMA/ionomycin. Representative flow plots are shown. The graph is of pooled data from n = 3 per condition (bars and error bars represent mean ±SEM. * P ≤ 0.05, ** P ≤ 0.001 (one-way ANOVA followed by Dunnett’s post-test with 0μM as control group). Data are representative of at least three independent experiments ( a–d,g ).

    Techniques Used: Staining, Flow Cytometry, Ex Vivo, Expressing, Cell Culture, Two Tailed Test

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    Cell Signaling Technology Inc anti pakt s473 af488

    Anti Pakt S473 Af488, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pakt s473 af488/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc rabbit anti pakt s473 af488

    Rabbit Anti Pakt S473 Af488, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti pakt s473 af488/product/Cell Signaling Technology Inc
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    Becton Dickinson anti pakt s473 af488
    Akt regulates development of both nT H 17 and iT H 17 cells. (a) Akt phosphorylation (p- Akt) at the <t>S473</t> site was determined for the indicated wild-type (WT) thymocyte populations by phospho-flow staining. Naive CD4 SP (CD4 SP CD44 lo CCR6 − ), nT reg (CD4 SP Foxp3 + ) and nT H 17 cells (CD4 SP CD44 hi CCR6 + ) were analyzed. (b) S6K phosphorylation (p-S6K) and S6 phosphorylation (p-S6) was assessed in the indicated thymocyte populations from WT mice by flow cytometry. The specificity of the phospho-flow staining was verified by treatment of thymocytes with rapamycin for 1 h at 37 °C prior to staining. (c) IL-17 is expressed in thymocytes from human fetal thymi upon ex vivo stimulation for 5 h with PMA/ionomycin and brefeldin A. Flow plots are gated on live lymphocytes (left) and CD4 SP cells (middle and right). (d) Phospho-flow analysis of p-Akt at the S473 site is shown for the indicated thymocyte populations from human fetal thymi. (e) Expression of IL-17A, ROR-γt, CCR6, and Foxp3 is shown for thymocytes from day 7 of E15-initiated FTOC, cultured for the last 2 days in the presence of indicated concentrations of allosteric Akt inhibitor, AKTi. Cultures were stimulated with PMA/ionomycin prior to staining. Representative flow plots are gated on CD4 SP TCRβ + TCRγδ − cells. Graphs show either the percent of IL-17A + or Foxp3 + cells among CD4 SP cells pooled from three independent experiments ( n = 5 thymi per condition; mean ± SEM; * P <0.0001, ** P =0.0124; P value from two-tailed Student’s t - test). (f) IL-17 production in thymocytes from WT and Myr-Akt mice following ex vivo stimulation was determined. Representative flow plots show staining on CD4 SP TCRβ + TCRγδ − gated cells. Graphs are of pooled data from two independent experiments ( n ≥ 5; mean ± SEM; * P =0.013, P value from two-tailed Student’s t -test). (g) WT naïve (CD44 lo CD62L hi CD25 − ) CD4 + T cells were activated for 18 h with anti- CD3 plus anti-CD28, followed by 36 h of culture in iT H 17-polarizing conditions in the presence of indicated concentrations of AKTi. IL-17 or Foxp3 expression was determined following restimulation with PMA/ionomycin. Representative flow plots are shown. The graph is of pooled data from n = 3 per condition (bars and error bars represent mean ±SEM. * P ≤ 0.05, ** P ≤ 0.001 (one-way ANOVA followed by Dunnett’s post-test with 0μM as control group). Data are representative of at least three independent experiments ( a–d,g ).
    Anti Pakt S473 Af488, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pakt s473 af488/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
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    Journal: Cell Reports

    Article Title: CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci

    doi: 10.1016/j.celrep.2020.108545

    Figure Lengend Snippet:

    Article Snippet: Finally, cells were pooled and stained with anti-CD3 BV510 (Biolegend, #300448), anti-CD4 PE (Biolegend, #357404), anti-CD8 AF700 (Biolegend, #300920), anti-pSTAT1-Y701 AF647 (Cell Signaling, #8009S), anti-pSTAT1-S727 AF488 (Biolegend, #686410), anti-pSTAT3-Y705 AF488 (Biolegend, #651006) and anti-pSTAT3-S727 AF647 (Biolegend, #698913), anti-pSTAT4-Y693 AF488 (BD Biosciences, #558136), anti-pSTAT5-Y694 AF647 (Cell Signaling, #9365S), anti-pSTAT6-Y641 AF488 (BD Biosciences, #612600), anti-pERK-T202/Y204 AF488 (eBiosciences, #53-9109-41), anti-pAKT-S473 AF488 (Cell Signaling, #4071S), anti-pAKT-T308 AF647 (Cell Signaling, #48646S), anti-pP90RSK-S380 AF488 (Cell Signaling, #13588S), anti-pS6R-S240/S244 AF488 (Cell Signaling, #5018S), anti-pS6R-S235/S236 AF647 (Cell Signaling, #4851S), anti-pZAP70-Y319/pSYK-Y352 AF647 (Cell Signaling, #82975S), anti-pCREB-S133 AF488 (Cell Signaling, #9187S), anti-pHIS3-S10 AF647 (Cell Signaling, #9716S), anti-pGSK3β-S9 AF647 (Cell Signaling, #14332S),anti-pCFOS-S32 AF647 (Cell Signaling, #8677S), anti-IRF1 AF647 (Cell Signaling, #14105S), anti-IRF4 AF647 (Biolegend, #646408), anti-IRF7 AF647 (Biolegend, #656007), anti-GATA3 AF488 (Biolegend, #653807), anti-TBET AF647 (Biolegend, #644803), anti-HIF1α AF488 (Biolegend, #359707), anti-MYC AF488 (Cell Signaling, #12855S), anti-O-GlcNAC AF647 (NOVUS Biologicals, #NB300-524AF647), anti-STAT3 APC (BD Biosciences, #560392) and anti-PLCγ1 AF647 (BD Biosciences, #557883).

    Techniques: Purification, Recombinant, Software

    Journal: Cell Reports

    Article Title: CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci

    doi: 10.1016/j.celrep.2020.108545

    Figure Lengend Snippet:

    Article Snippet: Rabbit anti-pAKT-S473-AF488 (Clone D9E) , Cell Signaling , Cat#4071S; RRID: AB_1031106.

    Techniques: Purification, Recombinant, Software

    Akt regulates development of both nT H 17 and iT H 17 cells. (a) Akt phosphorylation (p- Akt) at the S473 site was determined for the indicated wild-type (WT) thymocyte populations by phospho-flow staining. Naive CD4 SP (CD4 SP CD44 lo CCR6 − ), nT reg (CD4 SP Foxp3 + ) and nT H 17 cells (CD4 SP CD44 hi CCR6 + ) were analyzed. (b) S6K phosphorylation (p-S6K) and S6 phosphorylation (p-S6) was assessed in the indicated thymocyte populations from WT mice by flow cytometry. The specificity of the phospho-flow staining was verified by treatment of thymocytes with rapamycin for 1 h at 37 °C prior to staining. (c) IL-17 is expressed in thymocytes from human fetal thymi upon ex vivo stimulation for 5 h with PMA/ionomycin and brefeldin A. Flow plots are gated on live lymphocytes (left) and CD4 SP cells (middle and right). (d) Phospho-flow analysis of p-Akt at the S473 site is shown for the indicated thymocyte populations from human fetal thymi. (e) Expression of IL-17A, ROR-γt, CCR6, and Foxp3 is shown for thymocytes from day 7 of E15-initiated FTOC, cultured for the last 2 days in the presence of indicated concentrations of allosteric Akt inhibitor, AKTi. Cultures were stimulated with PMA/ionomycin prior to staining. Representative flow plots are gated on CD4 SP TCRβ + TCRγδ − cells. Graphs show either the percent of IL-17A + or Foxp3 + cells among CD4 SP cells pooled from three independent experiments ( n = 5 thymi per condition; mean ± SEM; * P <0.0001, ** P =0.0124; P value from two-tailed Student’s t - test). (f) IL-17 production in thymocytes from WT and Myr-Akt mice following ex vivo stimulation was determined. Representative flow plots show staining on CD4 SP TCRβ + TCRγδ − gated cells. Graphs are of pooled data from two independent experiments ( n ≥ 5; mean ± SEM; * P =0.013, P value from two-tailed Student’s t -test). (g) WT naïve (CD44 lo CD62L hi CD25 − ) CD4 + T cells were activated for 18 h with anti- CD3 plus anti-CD28, followed by 36 h of culture in iT H 17-polarizing conditions in the presence of indicated concentrations of AKTi. IL-17 or Foxp3 expression was determined following restimulation with PMA/ionomycin. Representative flow plots are shown. The graph is of pooled data from n = 3 per condition (bars and error bars represent mean ±SEM. * P ≤ 0.05, ** P ≤ 0.001 (one-way ANOVA followed by Dunnett’s post-test with 0μM as control group). Data are representative of at least three independent experiments ( a–d,g ).

    Journal: Nature immunology

    Article Title: Akt and mTOR pathways differentially regulate the development of natural and inducible T H 17 cells

    doi: 10.1038/ni.2607

    Figure Lengend Snippet: Akt regulates development of both nT H 17 and iT H 17 cells. (a) Akt phosphorylation (p- Akt) at the S473 site was determined for the indicated wild-type (WT) thymocyte populations by phospho-flow staining. Naive CD4 SP (CD4 SP CD44 lo CCR6 − ), nT reg (CD4 SP Foxp3 + ) and nT H 17 cells (CD4 SP CD44 hi CCR6 + ) were analyzed. (b) S6K phosphorylation (p-S6K) and S6 phosphorylation (p-S6) was assessed in the indicated thymocyte populations from WT mice by flow cytometry. The specificity of the phospho-flow staining was verified by treatment of thymocytes with rapamycin for 1 h at 37 °C prior to staining. (c) IL-17 is expressed in thymocytes from human fetal thymi upon ex vivo stimulation for 5 h with PMA/ionomycin and brefeldin A. Flow plots are gated on live lymphocytes (left) and CD4 SP cells (middle and right). (d) Phospho-flow analysis of p-Akt at the S473 site is shown for the indicated thymocyte populations from human fetal thymi. (e) Expression of IL-17A, ROR-γt, CCR6, and Foxp3 is shown for thymocytes from day 7 of E15-initiated FTOC, cultured for the last 2 days in the presence of indicated concentrations of allosteric Akt inhibitor, AKTi. Cultures were stimulated with PMA/ionomycin prior to staining. Representative flow plots are gated on CD4 SP TCRβ + TCRγδ − cells. Graphs show either the percent of IL-17A + or Foxp3 + cells among CD4 SP cells pooled from three independent experiments ( n = 5 thymi per condition; mean ± SEM; * P <0.0001, ** P =0.0124; P value from two-tailed Student’s t - test). (f) IL-17 production in thymocytes from WT and Myr-Akt mice following ex vivo stimulation was determined. Representative flow plots show staining on CD4 SP TCRβ + TCRγδ − gated cells. Graphs are of pooled data from two independent experiments ( n ≥ 5; mean ± SEM; * P =0.013, P value from two-tailed Student’s t -test). (g) WT naïve (CD44 lo CD62L hi CD25 − ) CD4 + T cells were activated for 18 h with anti- CD3 plus anti-CD28, followed by 36 h of culture in iT H 17-polarizing conditions in the presence of indicated concentrations of AKTi. IL-17 or Foxp3 expression was determined following restimulation with PMA/ionomycin. Representative flow plots are shown. The graph is of pooled data from n = 3 per condition (bars and error bars represent mean ±SEM. * P ≤ 0.05, ** P ≤ 0.001 (one-way ANOVA followed by Dunnett’s post-test with 0μM as control group). Data are representative of at least three independent experiments ( a–d,g ).

    Article Snippet: Surface stain was followed by permeabilization with Perm/Wash buffer (BD) and intracellular staining with antibodies including anti-pAkt(S473)-AF488 (BD, 56040), anti-pAkt(T308)-PE (BD, 558275), anti-pS6K (Cell Signaling; 9204), or anti-pS6 (Cell Signaling, 4856).

    Techniques: Staining, Flow Cytometry, Ex Vivo, Expressing, Cell Culture, Two Tailed Test