anti-p2x1 receptor antibody  (Alomone Labs)


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    Alomone Labs anti-p2x1 receptor antibody
    Anti P2x1 Receptor Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti p2x1  (Alomone Labs)


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    Alomone Labs anti p2x1
    Mast cell P2X receptor expression and modulation by IL-33. ( A – C ) Representative histogram indicating <t>P2X1</t> ( A ), P2X4 ( B ), and P2X7 ( C ) expression upon treatment with media (5 or 50 ng/mL IL-33 for 24 h). P2X1, P2X4, and P2X7 were stained and analysed by flow cytometry. The dotted lines indicate cell staining with isotype control antibodies. ( D – F ) IL-33-treated or untreated MCs were stained for P2X1 ( n = 2), P2X4 ( n = 5), and P2X7 ( n = 4); the geometric mean of fluorescence intensity (GMFI) was normalized to the negative control (untreated samples). Data are displayed as mean ± SEM. Statistical differences are indicated by * p < 0.05 and ** p < 0.01, *** p < 0.001 (one-way ANOVA with Šídák’s post hoc test).
    Anti P2x1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "P2X7 Receptor-Induced Human Mast Cell Degranulation Is Enhanced by Interleukin 33"

    Article Title: P2X7 Receptor-Induced Human Mast Cell Degranulation Is Enhanced by Interleukin 33

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms25031730

    Mast cell P2X receptor expression and modulation by IL-33. ( A – C ) Representative histogram indicating P2X1 ( A ), P2X4 ( B ), and P2X7 ( C ) expression upon treatment with media (5 or 50 ng/mL IL-33 for 24 h). P2X1, P2X4, and P2X7 were stained and analysed by flow cytometry. The dotted lines indicate cell staining with isotype control antibodies. ( D – F ) IL-33-treated or untreated MCs were stained for P2X1 ( n = 2), P2X4 ( n = 5), and P2X7 ( n = 4); the geometric mean of fluorescence intensity (GMFI) was normalized to the negative control (untreated samples). Data are displayed as mean ± SEM. Statistical differences are indicated by * p < 0.05 and ** p < 0.01, *** p < 0.001 (one-way ANOVA with Šídák’s post hoc test).
    Figure Legend Snippet: Mast cell P2X receptor expression and modulation by IL-33. ( A – C ) Representative histogram indicating P2X1 ( A ), P2X4 ( B ), and P2X7 ( C ) expression upon treatment with media (5 or 50 ng/mL IL-33 for 24 h). P2X1, P2X4, and P2X7 were stained and analysed by flow cytometry. The dotted lines indicate cell staining with isotype control antibodies. ( D – F ) IL-33-treated or untreated MCs were stained for P2X1 ( n = 2), P2X4 ( n = 5), and P2X7 ( n = 4); the geometric mean of fluorescence intensity (GMFI) was normalized to the negative control (untreated samples). Data are displayed as mean ± SEM. Statistical differences are indicated by * p < 0.05 and ** p < 0.01, *** p < 0.001 (one-way ANOVA with Šídák’s post hoc test).

    Techniques Used: Expressing, Staining, Flow Cytometry, Fluorescence, Negative Control

    The use of P2X1 and P2X4 receptors, alone or in combination, does not affect MC degranulation. MCs were left untreated or incubated with 5 ng/mL IL-33 for 24 h. ( A ) The MCs were exposed to NF449 (P2X1 inhibitor) and 5BDBD (P2X4 inhibitor) for 15 min before ATP stimulation at the concentrations indicated ( n = 3 separate experiments). A statistical analysis showed no significance between the control, stimulated, and IL-33-primed cells. ( B ) The MCs were exposed to NF449 (P2X1 inhibitor), 5BDBD (P2X4 inhibitor), and A438079 (P2X7 inhibitor) alone or in combination for 15 min before 1000 µM ATP stimulation at the concentrations indicated ( n = 3 separate experiments using different MC cultures). Statistical analysis showed no significant difference between the untreated controls and IL-33-primed cells. Data are presented as mean ± SEM. Statistical differences are indicated; * p < 0.05 (one-way ANOVA with Sidak’s post hoc test).
    Figure Legend Snippet: The use of P2X1 and P2X4 receptors, alone or in combination, does not affect MC degranulation. MCs were left untreated or incubated with 5 ng/mL IL-33 for 24 h. ( A ) The MCs were exposed to NF449 (P2X1 inhibitor) and 5BDBD (P2X4 inhibitor) for 15 min before ATP stimulation at the concentrations indicated ( n = 3 separate experiments). A statistical analysis showed no significance between the control, stimulated, and IL-33-primed cells. ( B ) The MCs were exposed to NF449 (P2X1 inhibitor), 5BDBD (P2X4 inhibitor), and A438079 (P2X7 inhibitor) alone or in combination for 15 min before 1000 µM ATP stimulation at the concentrations indicated ( n = 3 separate experiments using different MC cultures). Statistical analysis showed no significant difference between the untreated controls and IL-33-primed cells. Data are presented as mean ± SEM. Statistical differences are indicated; * p < 0.05 (one-way ANOVA with Sidak’s post hoc test).

    Techniques Used: Incubation

    rabbit anti p2x1 antibody  (Alomone Labs)


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    Alomone Labs rabbit anti p2x1 antibody
    Rabbit Anti P2x1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    polyclonal rabbit anti p2x1 7r antibodies  (Alomone Labs)


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    Alomone Labs polyclonal rabbit anti p2x1 7r antibodies
    Polyclonal Rabbit Anti P2x1 7r Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti-p2x1 receptor antibody  (Alomone Labs)


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    Alomone Labs anti-p2x1 receptor antibody
    Anti P2x1 Receptor Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti-p2x1 receptor antibody  (Alomone Labs)


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    Alomone Labs anti-p2x1 receptor antibody
    Anti P2x1 Receptor Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti p2x1 antibody  (Alomone Labs)


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    Alomone Labs rabbit anti p2x1 antibody
    Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and <t>P2X1</t> receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001
    Rabbit Anti P2x1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Molecular mechanisms of voiding dysfunction in a novel mouse model of acute urinary retention"

    Article Title: Molecular mechanisms of voiding dysfunction in a novel mouse model of acute urinary retention

    Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    doi: 10.1096/fj.202002415R

    Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and P2X1 receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001
    Figure Legend Snippet: Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and P2X1 receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001

    Techniques Used: Expressing, Western Blot

    western blot analysis rabbit anti p2x1 antibody  (Alomone Labs)


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    Alomone Labs western blot analysis rabbit anti p2x1 antibody
    Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and <t>P2X1</t> receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001
    Western Blot Analysis Rabbit Anti P2x1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Molecular mechanisms of voiding dysfunction in a novel mouse model of acute urinary retention"

    Article Title: Molecular mechanisms of voiding dysfunction in a novel mouse model of acute urinary retention

    Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    doi: 10.1096/fj.202002415R

    Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and P2X1 receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001
    Figure Legend Snippet: Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and P2X1 receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001

    Techniques Used: Expressing, Western Blot

    rabbit anti p2x1  (Alomone Labs)


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    Alomone Labs rabbit anti p2x1
    Rabbit Anti P2x1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti p2x1 6  (Alomone Labs)


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    Alomone Labs rabbit anti p2x1 6
    Expression and size distribution of <t>P2X1-positive</t> cells to total and FG-positive DRG neurons between the control and CCI groups. (a) P2X1-positive fluorescence images of total and FG-positive DRG between the control and CCI groups (200x, scale is 50 μ m). (b) The percentages of the expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. There was no significant difference between the control and CCI groups, n = 6.
    Rabbit Anti P2x1 6, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Retrograde Labeling of Different Distribution Features of DRG P2X2 and P2X3 Receptors in a Neuropathic Pain Rat Model"

    Article Title: Retrograde Labeling of Different Distribution Features of DRG P2X2 and P2X3 Receptors in a Neuropathic Pain Rat Model

    Journal: BioMed Research International

    doi: 10.1155/2020/9861459

    Expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. (a) P2X1-positive fluorescence images of total and FG-positive DRG between the control and CCI groups (200x, scale is 50 μ m). (b) The percentages of the expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. There was no significant difference between the control and CCI groups, n = 6.
    Figure Legend Snippet: Expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. (a) P2X1-positive fluorescence images of total and FG-positive DRG between the control and CCI groups (200x, scale is 50 μ m). (b) The percentages of the expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. There was no significant difference between the control and CCI groups, n = 6.

    Techniques Used: Expressing, Fluorescence

    rabbit anti p2x1  (Alomone Labs)


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    Alomone Labs rabbit anti p2x1
    ( A–D ) P2X7 expression analysed by Western blotting of macrophages isolated from control, EROS knockout (KO) ( A ) and gp91 phox KO mice ( B ), induced pluripotent stem cells (iPS)-derived macrophages control or EROS-deficient ( C ) and of control PLB985 cells and an EROS-deficient clone ( D ). ( E, F ) P2X7 expression in RAW264.7 cells overexpressing a FLAG-tagged EROS vector ( E ) and in HEK293 cells transiently expressing the specified constructs ( F ). ( G, H ) Interaction between EROS and P2X7 probed by immunoprecipitation (IP) of EROS from RAW264.7 EROS-FLAG macrophages followed by immunoblot (IB) for P2X7 ( G ) and by Nanoluc Binary Technology (NanoBIT) assay in live HEK293 cells expressing the LgBIT-fused EROS vector with a SmBIT-fused P2X7 vector ( H ). ( I ) <t>P2X1</t> expression in macrophages isolated from EROS KO mice compared to control. n = 5 biological replicates. ( J ) P2X1 abundance upon co-transfection with EROS construct in HEK293 cells. Data are representative of hree independent experiments; error bars indicate SEM of triplicates. See also and . Figure 5—source data 1. Raw unedited blots for . Figure 5—source data 2. Raw unedited blots for . Figure 5—source data 3. Raw unedited blots for . Figure 5—source data 4. Uncropped gels used for .
    Rabbit Anti P2x1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "EROS is a selective chaperone regulating the phagocyte NADPH oxidase and purinergic signalling"

    Article Title: EROS is a selective chaperone regulating the phagocyte NADPH oxidase and purinergic signalling

    Journal: eLife

    doi: 10.7554/eLife.76387

    ( A–D ) P2X7 expression analysed by Western blotting of macrophages isolated from control, EROS knockout (KO) ( A ) and gp91 phox KO mice ( B ), induced pluripotent stem cells (iPS)-derived macrophages control or EROS-deficient ( C ) and of control PLB985 cells and an EROS-deficient clone ( D ). ( E, F ) P2X7 expression in RAW264.7 cells overexpressing a FLAG-tagged EROS vector ( E ) and in HEK293 cells transiently expressing the specified constructs ( F ). ( G, H ) Interaction between EROS and P2X7 probed by immunoprecipitation (IP) of EROS from RAW264.7 EROS-FLAG macrophages followed by immunoblot (IB) for P2X7 ( G ) and by Nanoluc Binary Technology (NanoBIT) assay in live HEK293 cells expressing the LgBIT-fused EROS vector with a SmBIT-fused P2X7 vector ( H ). ( I ) P2X1 expression in macrophages isolated from EROS KO mice compared to control. n = 5 biological replicates. ( J ) P2X1 abundance upon co-transfection with EROS construct in HEK293 cells. Data are representative of hree independent experiments; error bars indicate SEM of triplicates. See also and . Figure 5—source data 1. Raw unedited blots for . Figure 5—source data 2. Raw unedited blots for . Figure 5—source data 3. Raw unedited blots for . Figure 5—source data 4. Uncropped gels used for .
    Figure Legend Snippet: ( A–D ) P2X7 expression analysed by Western blotting of macrophages isolated from control, EROS knockout (KO) ( A ) and gp91 phox KO mice ( B ), induced pluripotent stem cells (iPS)-derived macrophages control or EROS-deficient ( C ) and of control PLB985 cells and an EROS-deficient clone ( D ). ( E, F ) P2X7 expression in RAW264.7 cells overexpressing a FLAG-tagged EROS vector ( E ) and in HEK293 cells transiently expressing the specified constructs ( F ). ( G, H ) Interaction between EROS and P2X7 probed by immunoprecipitation (IP) of EROS from RAW264.7 EROS-FLAG macrophages followed by immunoblot (IB) for P2X7 ( G ) and by Nanoluc Binary Technology (NanoBIT) assay in live HEK293 cells expressing the LgBIT-fused EROS vector with a SmBIT-fused P2X7 vector ( H ). ( I ) P2X1 expression in macrophages isolated from EROS KO mice compared to control. n = 5 biological replicates. ( J ) P2X1 abundance upon co-transfection with EROS construct in HEK293 cells. Data are representative of hree independent experiments; error bars indicate SEM of triplicates. See also and . Figure 5—source data 1. Raw unedited blots for . Figure 5—source data 2. Raw unedited blots for . Figure 5—source data 3. Raw unedited blots for . Figure 5—source data 4. Uncropped gels used for .

    Techniques Used: Expressing, Western Blot, Isolation, Knock-Out, Derivative Assay, Plasmid Preparation, Construct, Immunoprecipitation, Cotransfection


    Figure Legend Snippet:

    Techniques Used: Knock-Out, Generated, Over Expression

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    Alomone Labs anti-p2x1 receptor antibody
    Anti P2x1 Receptor Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs anti p2x1
    Mast cell P2X receptor expression and modulation by IL-33. ( A – C ) Representative histogram indicating <t>P2X1</t> ( A ), P2X4 ( B ), and P2X7 ( C ) expression upon treatment with media (5 or 50 ng/mL IL-33 for 24 h). P2X1, P2X4, and P2X7 were stained and analysed by flow cytometry. The dotted lines indicate cell staining with isotype control antibodies. ( D – F ) IL-33-treated or untreated MCs were stained for P2X1 ( n = 2), P2X4 ( n = 5), and P2X7 ( n = 4); the geometric mean of fluorescence intensity (GMFI) was normalized to the negative control (untreated samples). Data are displayed as mean ± SEM. Statistical differences are indicated by * p < 0.05 and ** p < 0.01, *** p < 0.001 (one-way ANOVA with Šídák’s post hoc test).
    Anti P2x1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs rabbit anti p2x1 antibody
    Mast cell P2X receptor expression and modulation by IL-33. ( A – C ) Representative histogram indicating <t>P2X1</t> ( A ), P2X4 ( B ), and P2X7 ( C ) expression upon treatment with media (5 or 50 ng/mL IL-33 for 24 h). P2X1, P2X4, and P2X7 were stained and analysed by flow cytometry. The dotted lines indicate cell staining with isotype control antibodies. ( D – F ) IL-33-treated or untreated MCs were stained for P2X1 ( n = 2), P2X4 ( n = 5), and P2X7 ( n = 4); the geometric mean of fluorescence intensity (GMFI) was normalized to the negative control (untreated samples). Data are displayed as mean ± SEM. Statistical differences are indicated by * p < 0.05 and ** p < 0.01, *** p < 0.001 (one-way ANOVA with Šídák’s post hoc test).
    Rabbit Anti P2x1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs polyclonal rabbit anti p2x1 7r antibodies
    Mast cell P2X receptor expression and modulation by IL-33. ( A – C ) Representative histogram indicating <t>P2X1</t> ( A ), P2X4 ( B ), and P2X7 ( C ) expression upon treatment with media (5 or 50 ng/mL IL-33 for 24 h). P2X1, P2X4, and P2X7 were stained and analysed by flow cytometry. The dotted lines indicate cell staining with isotype control antibodies. ( D – F ) IL-33-treated or untreated MCs were stained for P2X1 ( n = 2), P2X4 ( n = 5), and P2X7 ( n = 4); the geometric mean of fluorescence intensity (GMFI) was normalized to the negative control (untreated samples). Data are displayed as mean ± SEM. Statistical differences are indicated by * p < 0.05 and ** p < 0.01, *** p < 0.001 (one-way ANOVA with Šídák’s post hoc test).
    Polyclonal Rabbit Anti P2x1 7r Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and <t>P2X1</t> receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001
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    Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and <t>P2X1</t> receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001
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    Expression and size distribution of <t>P2X1-positive</t> cells to total and FG-positive DRG neurons between the control and CCI groups. (a) P2X1-positive fluorescence images of total and FG-positive DRG between the control and CCI groups (200x, scale is 50 μ m). (b) The percentages of the expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. There was no significant difference between the control and CCI groups, n = 6.
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    Image Search Results


    Mast cell P2X receptor expression and modulation by IL-33. ( A – C ) Representative histogram indicating P2X1 ( A ), P2X4 ( B ), and P2X7 ( C ) expression upon treatment with media (5 or 50 ng/mL IL-33 for 24 h). P2X1, P2X4, and P2X7 were stained and analysed by flow cytometry. The dotted lines indicate cell staining with isotype control antibodies. ( D – F ) IL-33-treated or untreated MCs were stained for P2X1 ( n = 2), P2X4 ( n = 5), and P2X7 ( n = 4); the geometric mean of fluorescence intensity (GMFI) was normalized to the negative control (untreated samples). Data are displayed as mean ± SEM. Statistical differences are indicated by * p < 0.05 and ** p < 0.01, *** p < 0.001 (one-way ANOVA with Šídák’s post hoc test).

    Journal: International Journal of Molecular Sciences

    Article Title: P2X7 Receptor-Induced Human Mast Cell Degranulation Is Enhanced by Interleukin 33

    doi: 10.3390/ijms25031730

    Figure Lengend Snippet: Mast cell P2X receptor expression and modulation by IL-33. ( A – C ) Representative histogram indicating P2X1 ( A ), P2X4 ( B ), and P2X7 ( C ) expression upon treatment with media (5 or 50 ng/mL IL-33 for 24 h). P2X1, P2X4, and P2X7 were stained and analysed by flow cytometry. The dotted lines indicate cell staining with isotype control antibodies. ( D – F ) IL-33-treated or untreated MCs were stained for P2X1 ( n = 2), P2X4 ( n = 5), and P2X7 ( n = 4); the geometric mean of fluorescence intensity (GMFI) was normalized to the negative control (untreated samples). Data are displayed as mean ± SEM. Statistical differences are indicated by * p < 0.05 and ** p < 0.01, *** p < 0.001 (one-way ANOVA with Šídák’s post hoc test).

    Article Snippet: The cells were then washed in FACS buffer (PBS, 2% v / v FCS, 2 mM EDTA) and incubated with either anti-P2X1 (1 mg/mL) (Cat# APR-022; isotype rabbit IgG1), anti-P2X4 (1 mg/mL) (Cat# APR-024; isotype rabbit IgG1), or anti-P2X7 (1 mg/mL) (Cat# APR-008; isotype rabbit IgG1; Alomone labs, Jerusalem, Israel) primary antibodies, together with 5 µg/mL of Fc block.

    Techniques: Expressing, Staining, Flow Cytometry, Fluorescence, Negative Control

    The use of P2X1 and P2X4 receptors, alone or in combination, does not affect MC degranulation. MCs were left untreated or incubated with 5 ng/mL IL-33 for 24 h. ( A ) The MCs were exposed to NF449 (P2X1 inhibitor) and 5BDBD (P2X4 inhibitor) for 15 min before ATP stimulation at the concentrations indicated ( n = 3 separate experiments). A statistical analysis showed no significance between the control, stimulated, and IL-33-primed cells. ( B ) The MCs were exposed to NF449 (P2X1 inhibitor), 5BDBD (P2X4 inhibitor), and A438079 (P2X7 inhibitor) alone or in combination for 15 min before 1000 µM ATP stimulation at the concentrations indicated ( n = 3 separate experiments using different MC cultures). Statistical analysis showed no significant difference between the untreated controls and IL-33-primed cells. Data are presented as mean ± SEM. Statistical differences are indicated; * p < 0.05 (one-way ANOVA with Sidak’s post hoc test).

    Journal: International Journal of Molecular Sciences

    Article Title: P2X7 Receptor-Induced Human Mast Cell Degranulation Is Enhanced by Interleukin 33

    doi: 10.3390/ijms25031730

    Figure Lengend Snippet: The use of P2X1 and P2X4 receptors, alone or in combination, does not affect MC degranulation. MCs were left untreated or incubated with 5 ng/mL IL-33 for 24 h. ( A ) The MCs were exposed to NF449 (P2X1 inhibitor) and 5BDBD (P2X4 inhibitor) for 15 min before ATP stimulation at the concentrations indicated ( n = 3 separate experiments). A statistical analysis showed no significance between the control, stimulated, and IL-33-primed cells. ( B ) The MCs were exposed to NF449 (P2X1 inhibitor), 5BDBD (P2X4 inhibitor), and A438079 (P2X7 inhibitor) alone or in combination for 15 min before 1000 µM ATP stimulation at the concentrations indicated ( n = 3 separate experiments using different MC cultures). Statistical analysis showed no significant difference between the untreated controls and IL-33-primed cells. Data are presented as mean ± SEM. Statistical differences are indicated; * p < 0.05 (one-way ANOVA with Sidak’s post hoc test).

    Article Snippet: The cells were then washed in FACS buffer (PBS, 2% v / v FCS, 2 mM EDTA) and incubated with either anti-P2X1 (1 mg/mL) (Cat# APR-022; isotype rabbit IgG1), anti-P2X4 (1 mg/mL) (Cat# APR-024; isotype rabbit IgG1), or anti-P2X7 (1 mg/mL) (Cat# APR-008; isotype rabbit IgG1; Alomone labs, Jerusalem, Israel) primary antibodies, together with 5 µg/mL of Fc block.

    Techniques: Incubation

    Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and P2X1 receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001

    Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    Article Title: Molecular mechanisms of voiding dysfunction in a novel mouse model of acute urinary retention

    doi: 10.1096/fj.202002415R

    Figure Lengend Snippet: Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and P2X1 receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001

    Article Snippet: Western blot analysis Rabbit anti-P2X1 antibody (1:1000, #APR-001) and rabbit anti-chrm2 antibody (1:1000, #AMR-002) were purchased from Alomone Lab. Rabbit anti-chrm3 antibody (1:1000, #PA5–77485) was purchased from Thermo Fisher Scientific.

    Techniques: Expressing, Western Blot

    Expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. (a) P2X1-positive fluorescence images of total and FG-positive DRG between the control and CCI groups (200x, scale is 50 μ m). (b) The percentages of the expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. There was no significant difference between the control and CCI groups, n = 6.

    Journal: BioMed Research International

    Article Title: Retrograde Labeling of Different Distribution Features of DRG P2X2 and P2X3 Receptors in a Neuropathic Pain Rat Model

    doi: 10.1155/2020/9861459

    Figure Lengend Snippet: Expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. (a) P2X1-positive fluorescence images of total and FG-positive DRG between the control and CCI groups (200x, scale is 50 μ m). (b) The percentages of the expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. There was no significant difference between the control and CCI groups, n = 6.

    Article Snippet: The primary antibodies were rabbit anti-P2X1-6 (RRID/Cat: AB_2341048, #APR-022; AB_2341051, #APR-025; AB_2341052, #APR-026; AB_2341050, #APR-024; AB_2756757, #APR-027; and AB_2756758, #APR-028, respectively, diluted 1 : 200 with 0.1 M PBS, Alomone Labs, Israel).

    Techniques: Expressing, Fluorescence