anti p21 anti body  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti p21 anti body
    Cytoplasmic <t>p21</t> induced by p65 is necessary to prevent the activation of procaspase-3 in PANC1 cell . Cells were transiently transfected with or without over-expression of p65 and p65-targeted shRNA followed by DOX treatment (2 μg/ml) for 24 h. (A) The level of p65 protein was detected by Western blotting. (B) Cell survival was examined by CCK8 kit. Error bar indicates the standard error of the mean of three independent experiments. (C)The nuclear or cytoplasmic p21 protein (above) and pro-caspase-3 or active caspase-3 (bottom) were detected by Western blotting. GAPDH was used as controls.
    Anti P21 Anti Body, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p21 anti body/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p21 anti body - by Bioz Stars, 2024-06
    96/100 stars

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    1) Product Images from "Cytoplasmic p21 induced by p65 prevents doxorubicin-induced cell death in pancreatic carcinoma cell line"

    Article Title: Cytoplasmic p21 induced by p65 prevents doxorubicin-induced cell death in pancreatic carcinoma cell line

    Journal: Journal of Biomedical Science

    doi: 10.1186/1423-0127-19-15

    Cytoplasmic p21 induced by p65 is necessary to prevent the activation of procaspase-3 in PANC1 cell . Cells were transiently transfected with or without over-expression of p65 and p65-targeted shRNA followed by DOX treatment (2 μg/ml) for 24 h. (A) The level of p65 protein was detected by Western blotting. (B) Cell survival was examined by CCK8 kit. Error bar indicates the standard error of the mean of three independent experiments. (C)The nuclear or cytoplasmic p21 protein (above) and pro-caspase-3 or active caspase-3 (bottom) were detected by Western blotting. GAPDH was used as controls.
    Figure Legend Snippet: Cytoplasmic p21 induced by p65 is necessary to prevent the activation of procaspase-3 in PANC1 cell . Cells were transiently transfected with or without over-expression of p65 and p65-targeted shRNA followed by DOX treatment (2 μg/ml) for 24 h. (A) The level of p65 protein was detected by Western blotting. (B) Cell survival was examined by CCK8 kit. Error bar indicates the standard error of the mean of three independent experiments. (C)The nuclear or cytoplasmic p21 protein (above) and pro-caspase-3 or active caspase-3 (bottom) were detected by Western blotting. GAPDH was used as controls.

    Techniques Used: Activation Assay, Transfection, Over Expression, shRNA, Western Blot

    Pro-caspase-3 physically associated with cytoplasmic p21 induction by p65 in PANC1 cells . (A) Cells were transiently transfected with or without over-expression of p65 and p65-targeted shRNA followed by DOX treatment (2 μg/ml) for 24 h. Whole cell extract was subjected to immunoprecipitation with anti-p21 antibody and then immunoblotted with anti-pro-caspase-3 or -active-caspase-3 antibody. (B) Cells were transfected with vector expressing wild-type (1-585), N-terminal (1-372) and C-terminal (373-585) region of p21 followed by DOX treatment (2 μg/ml) for 24 h. Immunoprecipitation with an antibody against myc peptide. The pro-caspase-3 and p21 amounts were assessed by Western blotting.
    Figure Legend Snippet: Pro-caspase-3 physically associated with cytoplasmic p21 induction by p65 in PANC1 cells . (A) Cells were transiently transfected with or without over-expression of p65 and p65-targeted shRNA followed by DOX treatment (2 μg/ml) for 24 h. Whole cell extract was subjected to immunoprecipitation with anti-p21 antibody and then immunoblotted with anti-pro-caspase-3 or -active-caspase-3 antibody. (B) Cells were transfected with vector expressing wild-type (1-585), N-terminal (1-372) and C-terminal (373-585) region of p21 followed by DOX treatment (2 μg/ml) for 24 h. Immunoprecipitation with an antibody against myc peptide. The pro-caspase-3 and p21 amounts were assessed by Western blotting.

    Techniques Used: Transfection, Over Expression, shRNA, Immunoprecipitation, Plasmid Preparation, Expressing, Western Blot

    Induction of p65 enhanced the p53-mediated cell death response to DOX in PANC1 cells . Cells were transiently transfected with or without over-expression of p53 alone or p53 and p65 together followed by DOX treatment (2 μg/ml) for 24 h. (A) The level of p53 or p65 protein was detected by Western blotting. (B) Cell survival was examined by CCK8 kit. Error bar indicates the standard error of the mean of three independent experiments. (C)The nuclear or cytoplasmic p21 protein (above) or pro-caspase-3 (bottom) were detected by Western blotting. GAPDH was used as controls. Whole cell extract was subjected to immunoprecipitation with anti-p21 antibody and then immunoblotted with anti-pro-caspase-3 antibody (middle). (D) Active-caspase-3 and active-caspase-8 protein were detected by Western blotting. GAPDH was used as controls.
    Figure Legend Snippet: Induction of p65 enhanced the p53-mediated cell death response to DOX in PANC1 cells . Cells were transiently transfected with or without over-expression of p53 alone or p53 and p65 together followed by DOX treatment (2 μg/ml) for 24 h. (A) The level of p53 or p65 protein was detected by Western blotting. (B) Cell survival was examined by CCK8 kit. Error bar indicates the standard error of the mean of three independent experiments. (C)The nuclear or cytoplasmic p21 protein (above) or pro-caspase-3 (bottom) were detected by Western blotting. GAPDH was used as controls. Whole cell extract was subjected to immunoprecipitation with anti-p21 antibody and then immunoblotted with anti-pro-caspase-3 antibody (middle). (D) Active-caspase-3 and active-caspase-8 protein were detected by Western blotting. GAPDH was used as controls.

    Techniques Used: Transfection, Over Expression, Western Blot, Immunoprecipitation

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    Cell Signaling Technology Inc anti p21 anti body
    Cytoplasmic <t>p21</t> induced by p65 is necessary to prevent the activation of procaspase-3 in PANC1 cell . Cells were transiently transfected with or without over-expression of p65 and p65-targeted shRNA followed by DOX treatment (2 μg/ml) for 24 h. (A) The level of p65 protein was detected by Western blotting. (B) Cell survival was examined by CCK8 kit. Error bar indicates the standard error of the mean of three independent experiments. (C)The nuclear or cytoplasmic p21 protein (above) and pro-caspase-3 or active caspase-3 (bottom) were detected by Western blotting. GAPDH was used as controls.
    Anti P21 Anti Body, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p21 anti body/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p21 anti body - by Bioz Stars, 2024-06
    96/100 stars
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    Cytoplasmic p21 induced by p65 is necessary to prevent the activation of procaspase-3 in PANC1 cell . Cells were transiently transfected with or without over-expression of p65 and p65-targeted shRNA followed by DOX treatment (2 μg/ml) for 24 h. (A) The level of p65 protein was detected by Western blotting. (B) Cell survival was examined by CCK8 kit. Error bar indicates the standard error of the mean of three independent experiments. (C)The nuclear or cytoplasmic p21 protein (above) and pro-caspase-3 or active caspase-3 (bottom) were detected by Western blotting. GAPDH was used as controls.

    Journal: Journal of Biomedical Science

    Article Title: Cytoplasmic p21 induced by p65 prevents doxorubicin-induced cell death in pancreatic carcinoma cell line

    doi: 10.1186/1423-0127-19-15

    Figure Lengend Snippet: Cytoplasmic p21 induced by p65 is necessary to prevent the activation of procaspase-3 in PANC1 cell . Cells were transiently transfected with or without over-expression of p65 and p65-targeted shRNA followed by DOX treatment (2 μg/ml) for 24 h. (A) The level of p65 protein was detected by Western blotting. (B) Cell survival was examined by CCK8 kit. Error bar indicates the standard error of the mean of three independent experiments. (C)The nuclear or cytoplasmic p21 protein (above) and pro-caspase-3 or active caspase-3 (bottom) were detected by Western blotting. GAPDH was used as controls.

    Article Snippet: For immunoprecipitation, a 500 μg aliquot of cell lysate was incubated for 1 h at 4°C with the anti-p21 anti-body (2946, Cell Signaling).

    Techniques: Activation Assay, Transfection, Over Expression, shRNA, Western Blot

    Pro-caspase-3 physically associated with cytoplasmic p21 induction by p65 in PANC1 cells . (A) Cells were transiently transfected with or without over-expression of p65 and p65-targeted shRNA followed by DOX treatment (2 μg/ml) for 24 h. Whole cell extract was subjected to immunoprecipitation with anti-p21 antibody and then immunoblotted with anti-pro-caspase-3 or -active-caspase-3 antibody. (B) Cells were transfected with vector expressing wild-type (1-585), N-terminal (1-372) and C-terminal (373-585) region of p21 followed by DOX treatment (2 μg/ml) for 24 h. Immunoprecipitation with an antibody against myc peptide. The pro-caspase-3 and p21 amounts were assessed by Western blotting.

    Journal: Journal of Biomedical Science

    Article Title: Cytoplasmic p21 induced by p65 prevents doxorubicin-induced cell death in pancreatic carcinoma cell line

    doi: 10.1186/1423-0127-19-15

    Figure Lengend Snippet: Pro-caspase-3 physically associated with cytoplasmic p21 induction by p65 in PANC1 cells . (A) Cells were transiently transfected with or without over-expression of p65 and p65-targeted shRNA followed by DOX treatment (2 μg/ml) for 24 h. Whole cell extract was subjected to immunoprecipitation with anti-p21 antibody and then immunoblotted with anti-pro-caspase-3 or -active-caspase-3 antibody. (B) Cells were transfected with vector expressing wild-type (1-585), N-terminal (1-372) and C-terminal (373-585) region of p21 followed by DOX treatment (2 μg/ml) for 24 h. Immunoprecipitation with an antibody against myc peptide. The pro-caspase-3 and p21 amounts were assessed by Western blotting.

    Article Snippet: For immunoprecipitation, a 500 μg aliquot of cell lysate was incubated for 1 h at 4°C with the anti-p21 anti-body (2946, Cell Signaling).

    Techniques: Transfection, Over Expression, shRNA, Immunoprecipitation, Plasmid Preparation, Expressing, Western Blot

    Induction of p65 enhanced the p53-mediated cell death response to DOX in PANC1 cells . Cells were transiently transfected with or without over-expression of p53 alone or p53 and p65 together followed by DOX treatment (2 μg/ml) for 24 h. (A) The level of p53 or p65 protein was detected by Western blotting. (B) Cell survival was examined by CCK8 kit. Error bar indicates the standard error of the mean of three independent experiments. (C)The nuclear or cytoplasmic p21 protein (above) or pro-caspase-3 (bottom) were detected by Western blotting. GAPDH was used as controls. Whole cell extract was subjected to immunoprecipitation with anti-p21 antibody and then immunoblotted with anti-pro-caspase-3 antibody (middle). (D) Active-caspase-3 and active-caspase-8 protein were detected by Western blotting. GAPDH was used as controls.

    Journal: Journal of Biomedical Science

    Article Title: Cytoplasmic p21 induced by p65 prevents doxorubicin-induced cell death in pancreatic carcinoma cell line

    doi: 10.1186/1423-0127-19-15

    Figure Lengend Snippet: Induction of p65 enhanced the p53-mediated cell death response to DOX in PANC1 cells . Cells were transiently transfected with or without over-expression of p53 alone or p53 and p65 together followed by DOX treatment (2 μg/ml) for 24 h. (A) The level of p53 or p65 protein was detected by Western blotting. (B) Cell survival was examined by CCK8 kit. Error bar indicates the standard error of the mean of three independent experiments. (C)The nuclear or cytoplasmic p21 protein (above) or pro-caspase-3 (bottom) were detected by Western blotting. GAPDH was used as controls. Whole cell extract was subjected to immunoprecipitation with anti-p21 antibody and then immunoblotted with anti-pro-caspase-3 antibody (middle). (D) Active-caspase-3 and active-caspase-8 protein were detected by Western blotting. GAPDH was used as controls.

    Article Snippet: For immunoprecipitation, a 500 μg aliquot of cell lysate was incubated for 1 h at 4°C with the anti-p21 anti-body (2946, Cell Signaling).

    Techniques: Transfection, Over Expression, Western Blot, Immunoprecipitation