Structured Review

Becton Dickinson anti p190rhogap
Integrin β1-Arg signaling regulates <t>p190RhoGAP</t> activity a Immunoblot showing enrichment of Arg, p190RhoGAP (p190) and p120RasGAP (p120) in synaptoneurosomal fractions prepared from both WT and Nex-β1 −/− mice. PSD95 and actin are included as loading controls. b Representative examples of p190 immunoprecipitation with co-immunoprecipitated p120. c Quantification of p120RasGAP:p190RhoGAP complex. Complex level values represent p120 signal standardized to the amount of p190 in each IP compared to WT littermates. arg +/− Nex-β1 +/− and Nex-β1 −/− mice had significantly lower levels of p120RasGAP:p190RhoGAP complex than WT at P42. One-way ANOVA revealed no significant difference between genotypes at P21, but a significant effect of genotype at P42 [F(4,34)=3.856, p =0.011]. p120RasGAP:p190RhoGAP complex levels were reduced at P42 in both arg +/− Nex-β1 +/− and Nex-β1 −/− mice. Student's post-hoc t -test vs WT, P42 arg +/− Nex-β1 +/− p =0.001; P42 Nex-β1 −/− p =0.049, n = 7–14 mice for each genotype. d Representative examples of phospho-p190RhoGAP (pYp190) measurement from a p190 immunoprecipitation. e Quantification of p190RhoGAP phosphorylation levels from WT and mutant mice. One-way ANOVA revealed no significant difference between genotypes at P21, but a significant effect of genotype at P42 [F(2,16)=8.379, p =0.0032], with decreased pYp190 levels in both arg +/− Nex-β1 +/− and Nex-β1 −/− mice. Student's post-hoc t -test vs WT, P42 arg +/− Nex-β1 +/− p =0.002; P42 Nex-β1 −/− p =0.007, n = 4–8 mice for each genotype. f Quantification of Rho activity in arg +/− Nex-β1 +/− and Nex-β1 −/− mice. The amount of active Rho-GTP from each mutant mouse was compared to a WT littermate run in the same assay. Both arg +/− Nex-β1 +/− and Nex-β1 −/− mice had increased hippocampal Rho activity. One sample t -test comparing means to 1, P42 arg +/− Nex-β1 +/− p =0.02; P42 Nex-β1 −/− p =0.03. n =3–7 mice per group. Values represent mean ± SEM, * p
Anti P190rhogap, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p190rhogap/product/Becton Dickinson
Average 93 stars, based on 9 article reviews
Price from $9.99 to $1999.99
anti p190rhogap - by Bioz Stars, 2022-10
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Images

1) Product Images from "Integrin ?1 signals through Arg to regulate postnatal dendritic arborization, synapse density, and behavior"

Article Title: Integrin ?1 signals through Arg to regulate postnatal dendritic arborization, synapse density, and behavior

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.3942-11.2012

Integrin β1-Arg signaling regulates p190RhoGAP activity a Immunoblot showing enrichment of Arg, p190RhoGAP (p190) and p120RasGAP (p120) in synaptoneurosomal fractions prepared from both WT and Nex-β1 −/− mice. PSD95 and actin are included as loading controls. b Representative examples of p190 immunoprecipitation with co-immunoprecipitated p120. c Quantification of p120RasGAP:p190RhoGAP complex. Complex level values represent p120 signal standardized to the amount of p190 in each IP compared to WT littermates. arg +/− Nex-β1 +/− and Nex-β1 −/− mice had significantly lower levels of p120RasGAP:p190RhoGAP complex than WT at P42. One-way ANOVA revealed no significant difference between genotypes at P21, but a significant effect of genotype at P42 [F(4,34)=3.856, p =0.011]. p120RasGAP:p190RhoGAP complex levels were reduced at P42 in both arg +/− Nex-β1 +/− and Nex-β1 −/− mice. Student's post-hoc t -test vs WT, P42 arg +/− Nex-β1 +/− p =0.001; P42 Nex-β1 −/− p =0.049, n = 7–14 mice for each genotype. d Representative examples of phospho-p190RhoGAP (pYp190) measurement from a p190 immunoprecipitation. e Quantification of p190RhoGAP phosphorylation levels from WT and mutant mice. One-way ANOVA revealed no significant difference between genotypes at P21, but a significant effect of genotype at P42 [F(2,16)=8.379, p =0.0032], with decreased pYp190 levels in both arg +/− Nex-β1 +/− and Nex-β1 −/− mice. Student's post-hoc t -test vs WT, P42 arg +/− Nex-β1 +/− p =0.002; P42 Nex-β1 −/− p =0.007, n = 4–8 mice for each genotype. f Quantification of Rho activity in arg +/− Nex-β1 +/− and Nex-β1 −/− mice. The amount of active Rho-GTP from each mutant mouse was compared to a WT littermate run in the same assay. Both arg +/− Nex-β1 +/− and Nex-β1 −/− mice had increased hippocampal Rho activity. One sample t -test comparing means to 1, P42 arg +/− Nex-β1 +/− p =0.02; P42 Nex-β1 −/− p =0.03. n =3–7 mice per group. Values represent mean ± SEM, * p
Figure Legend Snippet: Integrin β1-Arg signaling regulates p190RhoGAP activity a Immunoblot showing enrichment of Arg, p190RhoGAP (p190) and p120RasGAP (p120) in synaptoneurosomal fractions prepared from both WT and Nex-β1 −/− mice. PSD95 and actin are included as loading controls. b Representative examples of p190 immunoprecipitation with co-immunoprecipitated p120. c Quantification of p120RasGAP:p190RhoGAP complex. Complex level values represent p120 signal standardized to the amount of p190 in each IP compared to WT littermates. arg +/− Nex-β1 +/− and Nex-β1 −/− mice had significantly lower levels of p120RasGAP:p190RhoGAP complex than WT at P42. One-way ANOVA revealed no significant difference between genotypes at P21, but a significant effect of genotype at P42 [F(4,34)=3.856, p =0.011]. p120RasGAP:p190RhoGAP complex levels were reduced at P42 in both arg +/− Nex-β1 +/− and Nex-β1 −/− mice. Student's post-hoc t -test vs WT, P42 arg +/− Nex-β1 +/− p =0.001; P42 Nex-β1 −/− p =0.049, n = 7–14 mice for each genotype. d Representative examples of phospho-p190RhoGAP (pYp190) measurement from a p190 immunoprecipitation. e Quantification of p190RhoGAP phosphorylation levels from WT and mutant mice. One-way ANOVA revealed no significant difference between genotypes at P21, but a significant effect of genotype at P42 [F(2,16)=8.379, p =0.0032], with decreased pYp190 levels in both arg +/− Nex-β1 +/− and Nex-β1 −/− mice. Student's post-hoc t -test vs WT, P42 arg +/− Nex-β1 +/− p =0.002; P42 Nex-β1 −/− p =0.007, n = 4–8 mice for each genotype. f Quantification of Rho activity in arg +/− Nex-β1 +/− and Nex-β1 −/− mice. The amount of active Rho-GTP from each mutant mouse was compared to a WT littermate run in the same assay. Both arg +/− Nex-β1 +/− and Nex-β1 −/− mice had increased hippocampal Rho activity. One sample t -test comparing means to 1, P42 arg +/− Nex-β1 +/− p =0.02; P42 Nex-β1 −/− p =0.03. n =3–7 mice per group. Values represent mean ± SEM, * p

Techniques Used: Activity Assay, Mouse Assay, Immunoprecipitation, Mutagenesis

Model for integrin β1-Arg signaling in dendritic spines (1) Integrin β1 is activated by binding to an unknown ligand. (2) The Arg kinase domain binds to the now exposed integrin β1 tail, stimulating Arg kinase activity by relieving autoinhibitory contacts between the SH3-SH2 domains and the kinase domain. (3) Arg phosphorylates p190RhoGAP, promoting its association with p120RasGAP at the membrane. (4) The p120RasGAP:p190R hoGAP complex inhibits RhoA, stabilizing synapses and dendrites.
Figure Legend Snippet: Model for integrin β1-Arg signaling in dendritic spines (1) Integrin β1 is activated by binding to an unknown ligand. (2) The Arg kinase domain binds to the now exposed integrin β1 tail, stimulating Arg kinase activity by relieving autoinhibitory contacts between the SH3-SH2 domains and the kinase domain. (3) Arg phosphorylates p190RhoGAP, promoting its association with p120RasGAP at the membrane. (4) The p120RasGAP:p190R hoGAP complex inhibits RhoA, stabilizing synapses and dendrites.

Techniques Used: Binding Assay, Activity Assay

2) Product Images from "Signaling Pathways That Control Rho Kinase Activity Maintain the Embryonic Epicardial Progenitor State"

Article Title: Signaling Pathways That Control Rho Kinase Activity Maintain the Embryonic Epicardial Progenitor State

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M114.613190

The cAMP target, Epac, suppresses EMT and cell migration in epicardial cell outgrowth from heart explants. A , Western blot showing that Epac1, p190RhoGAP, and Rap1 proteins were expressed in EMCs and the PE. B , the Epac activator, 007 (50 μ m ),
Figure Legend Snippet: The cAMP target, Epac, suppresses EMT and cell migration in epicardial cell outgrowth from heart explants. A , Western blot showing that Epac1, p190RhoGAP, and Rap1 proteins were expressed in EMCs and the PE. B , the Epac activator, 007 (50 μ m ),

Techniques Used: Migration, Western Blot

Rnd3 associates with p190RhoGAP in epicardial cells in the epithelial state; overall signaling scheme. A , anti-p190RhoGAP antibody, but not a nonspecific mouse IgG antibody, coimmunoprecipitated ( IP ) endogenous p190RhoGAP and Rnd 3. Anti-Rnd3 antibody,
Figure Legend Snippet: Rnd3 associates with p190RhoGAP in epicardial cells in the epithelial state; overall signaling scheme. A , anti-p190RhoGAP antibody, but not a nonspecific mouse IgG antibody, coimmunoprecipitated ( IP ) endogenous p190RhoGAP and Rnd 3. Anti-Rnd3 antibody,

Techniques Used:

3) Product Images from "RhoA is required for cortical retraction and rigidity during mitotic cell rounding"

Article Title: RhoA is required for cortical retraction and rigidity during mitotic cell rounding

Journal: The Journal of Cell Biology

doi: 10.1083/jcb.200207130

p190RhoGAP is tyrosine dephosphorylated, serine/threonine phosphorylated, and decreased in activity in mitosis. (A) p190RhoGAP was immunoprecipitated from HeLa cells and probed, stripped, and reprobed for p190RhoGAP, phosphotyrosine, and coassociation with p120RasGAP. Note the decrease in phosphotyrosine and p120RasGAP associated with mitotic p190RhoGAP, and the electrophoretic mobility shift in mitosis. (B) p190RhoGAP was immunoprecipitated from interphase and mitotic HeLa cells, and immunoprecipitates were washed into PP1 reaction buffer and divided into four equal samples. One sample was not treated (−); the remaining three each received 1 U PP1 phosphatase. The serine/threonine phosphatase inhibitor okadaic acid (PP1 + O) and the tyrosine phosphatase inhibitor vanadate (PP1 + V) were added to one sample each before PP1 was added. Note that the retarded electrophoretic mobility of mitotic p190RhoGAP is restored to the interphase mobility by PP1 treatment. (C) p190RhoGAP activity is lower in mitosis than in interphase as assayed for RhoA GAP activity. PP1 phosphatase treatment increases the activity of mitotic p190RhoGAP. Graphed are means from six experiments. Bars represent the SEM. *, significant difference from Interphase (−); **, significant difference from Mitosis (−) (P
Figure Legend Snippet: p190RhoGAP is tyrosine dephosphorylated, serine/threonine phosphorylated, and decreased in activity in mitosis. (A) p190RhoGAP was immunoprecipitated from HeLa cells and probed, stripped, and reprobed for p190RhoGAP, phosphotyrosine, and coassociation with p120RasGAP. Note the decrease in phosphotyrosine and p120RasGAP associated with mitotic p190RhoGAP, and the electrophoretic mobility shift in mitosis. (B) p190RhoGAP was immunoprecipitated from interphase and mitotic HeLa cells, and immunoprecipitates were washed into PP1 reaction buffer and divided into four equal samples. One sample was not treated (−); the remaining three each received 1 U PP1 phosphatase. The serine/threonine phosphatase inhibitor okadaic acid (PP1 + O) and the tyrosine phosphatase inhibitor vanadate (PP1 + V) were added to one sample each before PP1 was added. Note that the retarded electrophoretic mobility of mitotic p190RhoGAP is restored to the interphase mobility by PP1 treatment. (C) p190RhoGAP activity is lower in mitosis than in interphase as assayed for RhoA GAP activity. PP1 phosphatase treatment increases the activity of mitotic p190RhoGAP. Graphed are means from six experiments. Bars represent the SEM. *, significant difference from Interphase (−); **, significant difference from Mitosis (−) (P

Techniques Used: Activity Assay, Immunoprecipitation, Electrophoretic Mobility Shift Assay

Transient overexpression of GFP–p190RhoGAP does not block mitotic cell rounding. At 24 h after transfection, HeLa cells were fixed, stained, imaged, and measured as for Figs. 1 and 2. Also measured was the total GFP fluorescence within a circular region of fixed size, which comprised much of the cytoplasm. (A) A metaphase cell expressing GFP–p190RhoGAP has undergone mitotic cell rounding to a similar extent as a neighboring cell expressing little or no GFP–p190RhoGAP. (B) The extent of mitotic cell rounding, measured as cell diameter, perimeter, and area, was plotted against the intensity of the cytoplasmic GFP signal. GFP fluorescence is not predictive of the three cell rounding measurements.
Figure Legend Snippet: Transient overexpression of GFP–p190RhoGAP does not block mitotic cell rounding. At 24 h after transfection, HeLa cells were fixed, stained, imaged, and measured as for Figs. 1 and 2. Also measured was the total GFP fluorescence within a circular region of fixed size, which comprised much of the cytoplasm. (A) A metaphase cell expressing GFP–p190RhoGAP has undergone mitotic cell rounding to a similar extent as a neighboring cell expressing little or no GFP–p190RhoGAP. (B) The extent of mitotic cell rounding, measured as cell diameter, perimeter, and area, was plotted against the intensity of the cytoplasmic GFP signal. GFP fluorescence is not predictive of the three cell rounding measurements.

Techniques Used: Over Expression, Blocking Assay, Transfection, Staining, Fluorescence, Expressing

4) Product Images from "An Adaptor Role for Cytoplasmic Sam68 in Modulating Src Activity during Cell Polarization ▿"

Article Title: An Adaptor Role for Cytoplasmic Sam68 in Modulating Src Activity during Cell Polarization ▿

Journal: Molecular and Cellular Biology

doi: 10.1128/MCB.01707-08

Sustained Src and p190RhoGAP activities in Sam68 −/− MEFs. (A) Cells were plated for the indicated time on fibronectin-coated dishes, and cell extracts were prepared. p190RhoGAP was immunoprecipitated using anti-p190RhoGAP antibodies, and
Figure Legend Snippet: Sustained Src and p190RhoGAP activities in Sam68 −/− MEFs. (A) Cells were plated for the indicated time on fibronectin-coated dishes, and cell extracts were prepared. p190RhoGAP was immunoprecipitated using anti-p190RhoGAP antibodies, and

Techniques Used: Immunoprecipitation

5) Product Images from "Arp2/3-Branched Actin Maintains an Active Pool of GTP-RhoA and Controls RhoA Abundance"

Article Title: Arp2/3-Branched Actin Maintains an Active Pool of GTP-RhoA and Controls RhoA Abundance

Journal: Cells

doi: 10.3390/cells8101264

p190RhoGAP is responsible for reduced RhoA activity upon inhibition of the Arp2/3-branched actin. ( A ) Representative immunofluorescent images of p190RhoGAP in MEFs treated with DMSO or CK-666, with red: p190RhoGAP, blue: nucleus, and scale bar of 20 μm. ( B ) Western blot showing GTP-RhoA levels with or without p190RhoGAP: GAPDH was used as loading control, error bar indicates SEM, * p
Figure Legend Snippet: p190RhoGAP is responsible for reduced RhoA activity upon inhibition of the Arp2/3-branched actin. ( A ) Representative immunofluorescent images of p190RhoGAP in MEFs treated with DMSO or CK-666, with red: p190RhoGAP, blue: nucleus, and scale bar of 20 μm. ( B ) Western blot showing GTP-RhoA levels with or without p190RhoGAP: GAPDH was used as loading control, error bar indicates SEM, * p

Techniques Used: Activity Assay, Inhibition, Western Blot

6) Product Images from "HIV-1 Nef Disrupts the Podocyte Actin Cytoskeleton by Interacting with Diaphanous Interacting Protein *"

Article Title: HIV-1 Nef Disrupts the Podocyte Actin Cytoskeleton by Interacting with Diaphanous Interacting Protein *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M708920200

DIP interacts with p190RhoGAP and Vav2 in podocytes. Lysates from control and Nef podocytes were immunoprecipitated ( IP ) with anti-DIP and immunoblotted ( IB ) with anti-p190 or anti-Vav2. DIP co-precipitates with both p190RhoGAP and Vav2 in both control
Figure Legend Snippet: DIP interacts with p190RhoGAP and Vav2 in podocytes. Lysates from control and Nef podocytes were immunoprecipitated ( IP ) with anti-DIP and immunoblotted ( IB ) with anti-p190 or anti-Vav2. DIP co-precipitates with both p190RhoGAP and Vav2 in both control

Techniques Used: Immunoprecipitation

A , DIP dominant negative ( DN ) blocks Nef-induced p190RhoGAP phosphorylation. 293T cells were transfected with either control, Nef, or Nef P XX P along with either control or DIPΔPro. Control with EGF was used as a positive control. Pull-down
Figure Legend Snippet: A , DIP dominant negative ( DN ) blocks Nef-induced p190RhoGAP phosphorylation. 293T cells were transfected with either control, Nef, or Nef P XX P along with either control or DIPΔPro. Control with EGF was used as a positive control. Pull-down

Techniques Used: Dominant Negative Mutation, Transfection, Positive Control

Nef interacts with Src, DIP, and Vav2. DIP interacts with both Nef and p190RhoAGAP, mediating Nef-Src-induced phosphorylation of p190RhoGAP. Nef interacts directly with Vav2 to induce Src-mediated Vav2 phosphorylation. The interaction between DIP and
Figure Legend Snippet: Nef interacts with Src, DIP, and Vav2. DIP interacts with both Nef and p190RhoAGAP, mediating Nef-Src-induced phosphorylation of p190RhoGAP. Nef interacts directly with Vav2 to induce Src-mediated Vav2 phosphorylation. The interaction between DIP and

Techniques Used:

7) Product Images from "Arp2/3-Branched Actin Maintains an Active Pool of GTP-RhoA and Controls RhoA Abundance"

Article Title: Arp2/3-Branched Actin Maintains an Active Pool of GTP-RhoA and Controls RhoA Abundance

Journal: Cells

doi: 10.3390/cells8101264

p190RhoGAP is responsible for reduced RhoA activity upon inhibition of the Arp2/3-branched actin. ( A ) Representative immunofluorescent images of p190RhoGAP in MEFs treated with DMSO or CK-666, with red: p190RhoGAP, blue: nucleus, and scale bar of 20 μm. ( B ) Western blot showing GTP-RhoA levels with or without p190RhoGAP: GAPDH was used as loading control, error bar indicates SEM, * p
Figure Legend Snippet: p190RhoGAP is responsible for reduced RhoA activity upon inhibition of the Arp2/3-branched actin. ( A ) Representative immunofluorescent images of p190RhoGAP in MEFs treated with DMSO or CK-666, with red: p190RhoGAP, blue: nucleus, and scale bar of 20 μm. ( B ) Western blot showing GTP-RhoA levels with or without p190RhoGAP: GAPDH was used as loading control, error bar indicates SEM, * p

Techniques Used: Activity Assay, Inhibition, Western Blot

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  • 93
    Becton Dickinson p190rhogap
    Early onset of MBP expression in the brain of Ptprz -deficient mice. A , Schematic drawing of postulated signaling mechanisms of Ptprz and Fyn in oligodendrocyte differentiation and myelination. Fyn and Ptprz may also act on yet unidentified substrates other than <t>p190RhoGAP</t> to regulate the differentiation. The red arrow shows activation, whereas the blunt blue arrows represent inhibition. B , C , Western blot analyses of MBP expression in the cerebral cortex of mice at postnatal day 10 (B), and 3 months old (C). Applied protein amounts were verified by Coomassie Brilliant Blue (CBB) staining. The amounts of MBP are presented as densitometric units normalized to the value for respective wild-type controls, and are shown at the lower position of each panel. Data are the mean ± SEM ( n = 6 for each group). ** p
    P190rhogap, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p190rhogap/product/Becton Dickinson
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p190rhogap - by Bioz Stars, 2022-10
    93/100 stars
      Buy from Supplier

    88
    Becton Dickinson anti p190rhogap
    Integrin β1-Arg signaling regulates <t>p190RhoGAP</t> activity a Immunoblot showing enrichment of Arg, p190RhoGAP (p190) and p120RasGAP (p120) in synaptoneurosomal fractions prepared from both WT and Nex-β1 −/− mice. PSD95 and actin are included as loading controls. b Representative examples of p190 immunoprecipitation with co-immunoprecipitated p120. c Quantification of p120RasGAP:p190RhoGAP complex. Complex level values represent p120 signal standardized to the amount of p190 in each IP compared to WT littermates. arg +/− Nex-β1 +/− and Nex-β1 −/− mice had significantly lower levels of p120RasGAP:p190RhoGAP complex than WT at P42. One-way ANOVA revealed no significant difference between genotypes at P21, but a significant effect of genotype at P42 [F(4,34)=3.856, p =0.011]. p120RasGAP:p190RhoGAP complex levels were reduced at P42 in both arg +/− Nex-β1 +/− and Nex-β1 −/− mice. Student's post-hoc t -test vs WT, P42 arg +/− Nex-β1 +/− p =0.001; P42 Nex-β1 −/− p =0.049, n = 7–14 mice for each genotype. d Representative examples of phospho-p190RhoGAP (pYp190) measurement from a p190 immunoprecipitation. e Quantification of p190RhoGAP phosphorylation levels from WT and mutant mice. One-way ANOVA revealed no significant difference between genotypes at P21, but a significant effect of genotype at P42 [F(2,16)=8.379, p =0.0032], with decreased pYp190 levels in both arg +/− Nex-β1 +/− and Nex-β1 −/− mice. Student's post-hoc t -test vs WT, P42 arg +/− Nex-β1 +/− p =0.002; P42 Nex-β1 −/− p =0.007, n = 4–8 mice for each genotype. f Quantification of Rho activity in arg +/− Nex-β1 +/− and Nex-β1 −/− mice. The amount of active Rho-GTP from each mutant mouse was compared to a WT littermate run in the same assay. Both arg +/− Nex-β1 +/− and Nex-β1 −/− mice had increased hippocampal Rho activity. One sample t -test comparing means to 1, P42 arg +/− Nex-β1 +/− p =0.02; P42 Nex-β1 −/− p =0.03. n =3–7 mice per group. Values represent mean ± SEM, * p
    Anti P190rhogap, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p190rhogap/product/Becton Dickinson
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p190rhogap - by Bioz Stars, 2022-10
    88/100 stars
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    99
    Becton Dickinson monoclonal anti p190rhogap
    DIP interacts with <t>p190RhoGAP</t> and Vav2 in podocytes. Lysates from control and Nef podocytes were immunoprecipitated ( IP ) with anti-DIP and immunoblotted ( IB ) with anti-p190 or anti-Vav2. DIP co-precipitates with both p190RhoGAP and Vav2 in both control
    Monoclonal Anti P190rhogap, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal anti p190rhogap/product/Becton Dickinson
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal anti p190rhogap - by Bioz Stars, 2022-10
    99/100 stars
      Buy from Supplier

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    Early onset of MBP expression in the brain of Ptprz -deficient mice. A , Schematic drawing of postulated signaling mechanisms of Ptprz and Fyn in oligodendrocyte differentiation and myelination. Fyn and Ptprz may also act on yet unidentified substrates other than p190RhoGAP to regulate the differentiation. The red arrow shows activation, whereas the blunt blue arrows represent inhibition. B , C , Western blot analyses of MBP expression in the cerebral cortex of mice at postnatal day 10 (B), and 3 months old (C). Applied protein amounts were verified by Coomassie Brilliant Blue (CBB) staining. The amounts of MBP are presented as densitometric units normalized to the value for respective wild-type controls, and are shown at the lower position of each panel. Data are the mean ± SEM ( n = 6 for each group). ** p

    Journal: PLoS ONE

    Article Title: Protein Tyrosine Phosphatase Receptor Type Z Negatively Regulates Oligodendrocyte Differentiation and Myelination

    doi: 10.1371/journal.pone.0048797

    Figure Lengend Snippet: Early onset of MBP expression in the brain of Ptprz -deficient mice. A , Schematic drawing of postulated signaling mechanisms of Ptprz and Fyn in oligodendrocyte differentiation and myelination. Fyn and Ptprz may also act on yet unidentified substrates other than p190RhoGAP to regulate the differentiation. The red arrow shows activation, whereas the blunt blue arrows represent inhibition. B , C , Western blot analyses of MBP expression in the cerebral cortex of mice at postnatal day 10 (B), and 3 months old (C). Applied protein amounts were verified by Coomassie Brilliant Blue (CBB) staining. The amounts of MBP are presented as densitometric units normalized to the value for respective wild-type controls, and are shown at the lower position of each panel. Data are the mean ± SEM ( n = 6 for each group). ** p

    Article Snippet: We used commercially available antibodies against phosphotyrosine (PY20, GE Healthcare), MBP (cat no. sc-13914; Santa Cruz), p190RhoGAP (cat no. 610150; BD Biosciences), the intracellular region of Ptprz (anti-RPTPβ, cat no. 610180; BD Biosciences), Fyn (cat no. P2992; Sigma-Aldrich), phosphorylated Tyr 420 of Fyn (cat no. 2101; Cell signaling), phosphorylated Tyr 531 of Fyn (cat no. 2105; Cell signaling), Iba1 (cat no. 019-19741; Wako Pure Chemical), CD3 (cat no. ab5690; abcam), Olig2 (cat no. AF2418; R & D systems), and NG2 chondroitin sulfate proteoglycan (cat no AB5320; Millipore).

    Techniques: Expressing, Mouse Assay, Activated Clotting Time Assay, Activation Assay, Inhibition, Western Blot, Staining

    Increased phosphorylation of Tyr 1105 on p190RhoGAP in the spinal cord of Ptprz -deficient mice after EAE induction. A , Overall tyrosine phosphorylation patterns of total protein and expression of p190RhoGAP and Fyn in the spinal cord. The third to sixth lumbar spinal cord extracts were prepared from wild-type (+/+) and Ptprz -deficient mice (−/−) 35 days after MOG immunization, or non-immunized control animals, and examined by Western blotting using anti-phosphotyrosine PY20 (top), anti-p190RhoGAP (middle), and anti-Fyn (bottom) antibodies, respectively. B , Tyrosine phosphorylation of Tyr 1105 on p190RhoGAP. The spinal cord extracts were immunoprecipitated with anti-p190RhoGAP antibody and immunoblotted with anti-pY1105 p190RhoGAP (upper), or anti-p190RhoGAP (lower). The densitometric data for anti-pY1105 p190RhoGAP signals are presented as a percentage of the non-immunized wild-type control, and shown at the bottom. Data are the mean ± SEM ( n = 4 pooled samples from two animals per each group). * p

    Journal: PLoS ONE

    Article Title: Protein Tyrosine Phosphatase Receptor Type Z Negatively Regulates Oligodendrocyte Differentiation and Myelination

    doi: 10.1371/journal.pone.0048797

    Figure Lengend Snippet: Increased phosphorylation of Tyr 1105 on p190RhoGAP in the spinal cord of Ptprz -deficient mice after EAE induction. A , Overall tyrosine phosphorylation patterns of total protein and expression of p190RhoGAP and Fyn in the spinal cord. The third to sixth lumbar spinal cord extracts were prepared from wild-type (+/+) and Ptprz -deficient mice (−/−) 35 days after MOG immunization, or non-immunized control animals, and examined by Western blotting using anti-phosphotyrosine PY20 (top), anti-p190RhoGAP (middle), and anti-Fyn (bottom) antibodies, respectively. B , Tyrosine phosphorylation of Tyr 1105 on p190RhoGAP. The spinal cord extracts were immunoprecipitated with anti-p190RhoGAP antibody and immunoblotted with anti-pY1105 p190RhoGAP (upper), or anti-p190RhoGAP (lower). The densitometric data for anti-pY1105 p190RhoGAP signals are presented as a percentage of the non-immunized wild-type control, and shown at the bottom. Data are the mean ± SEM ( n = 4 pooled samples from two animals per each group). * p

    Article Snippet: We used commercially available antibodies against phosphotyrosine (PY20, GE Healthcare), MBP (cat no. sc-13914; Santa Cruz), p190RhoGAP (cat no. 610150; BD Biosciences), the intracellular region of Ptprz (anti-RPTPβ, cat no. 610180; BD Biosciences), Fyn (cat no. P2992; Sigma-Aldrich), phosphorylated Tyr 420 of Fyn (cat no. 2101; Cell signaling), phosphorylated Tyr 531 of Fyn (cat no. 2105; Cell signaling), Iba1 (cat no. 019-19741; Wako Pure Chemical), CD3 (cat no. ab5690; abcam), Olig2 (cat no. AF2418; R & D systems), and NG2 chondroitin sulfate proteoglycan (cat no AB5320; Millipore).

    Techniques: Mouse Assay, Expressing, Western Blot, Immunoprecipitation

    Integrin β1-Arg signaling regulates p190RhoGAP activity a Immunoblot showing enrichment of Arg, p190RhoGAP (p190) and p120RasGAP (p120) in synaptoneurosomal fractions prepared from both WT and Nex-β1 −/− mice. PSD95 and actin are included as loading controls. b Representative examples of p190 immunoprecipitation with co-immunoprecipitated p120. c Quantification of p120RasGAP:p190RhoGAP complex. Complex level values represent p120 signal standardized to the amount of p190 in each IP compared to WT littermates. arg +/− Nex-β1 +/− and Nex-β1 −/− mice had significantly lower levels of p120RasGAP:p190RhoGAP complex than WT at P42. One-way ANOVA revealed no significant difference between genotypes at P21, but a significant effect of genotype at P42 [F(4,34)=3.856, p =0.011]. p120RasGAP:p190RhoGAP complex levels were reduced at P42 in both arg +/− Nex-β1 +/− and Nex-β1 −/− mice. Student's post-hoc t -test vs WT, P42 arg +/− Nex-β1 +/− p =0.001; P42 Nex-β1 −/− p =0.049, n = 7–14 mice for each genotype. d Representative examples of phospho-p190RhoGAP (pYp190) measurement from a p190 immunoprecipitation. e Quantification of p190RhoGAP phosphorylation levels from WT and mutant mice. One-way ANOVA revealed no significant difference between genotypes at P21, but a significant effect of genotype at P42 [F(2,16)=8.379, p =0.0032], with decreased pYp190 levels in both arg +/− Nex-β1 +/− and Nex-β1 −/− mice. Student's post-hoc t -test vs WT, P42 arg +/− Nex-β1 +/− p =0.002; P42 Nex-β1 −/− p =0.007, n = 4–8 mice for each genotype. f Quantification of Rho activity in arg +/− Nex-β1 +/− and Nex-β1 −/− mice. The amount of active Rho-GTP from each mutant mouse was compared to a WT littermate run in the same assay. Both arg +/− Nex-β1 +/− and Nex-β1 −/− mice had increased hippocampal Rho activity. One sample t -test comparing means to 1, P42 arg +/− Nex-β1 +/− p =0.02; P42 Nex-β1 −/− p =0.03. n =3–7 mice per group. Values represent mean ± SEM, * p

    Journal: The Journal of Neuroscience

    Article Title: Integrin ?1 signals through Arg to regulate postnatal dendritic arborization, synapse density, and behavior

    doi: 10.1523/JNEUROSCI.3942-11.2012

    Figure Lengend Snippet: Integrin β1-Arg signaling regulates p190RhoGAP activity a Immunoblot showing enrichment of Arg, p190RhoGAP (p190) and p120RasGAP (p120) in synaptoneurosomal fractions prepared from both WT and Nex-β1 −/− mice. PSD95 and actin are included as loading controls. b Representative examples of p190 immunoprecipitation with co-immunoprecipitated p120. c Quantification of p120RasGAP:p190RhoGAP complex. Complex level values represent p120 signal standardized to the amount of p190 in each IP compared to WT littermates. arg +/− Nex-β1 +/− and Nex-β1 −/− mice had significantly lower levels of p120RasGAP:p190RhoGAP complex than WT at P42. One-way ANOVA revealed no significant difference between genotypes at P21, but a significant effect of genotype at P42 [F(4,34)=3.856, p =0.011]. p120RasGAP:p190RhoGAP complex levels were reduced at P42 in both arg +/− Nex-β1 +/− and Nex-β1 −/− mice. Student's post-hoc t -test vs WT, P42 arg +/− Nex-β1 +/− p =0.001; P42 Nex-β1 −/− p =0.049, n = 7–14 mice for each genotype. d Representative examples of phospho-p190RhoGAP (pYp190) measurement from a p190 immunoprecipitation. e Quantification of p190RhoGAP phosphorylation levels from WT and mutant mice. One-way ANOVA revealed no significant difference between genotypes at P21, but a significant effect of genotype at P42 [F(2,16)=8.379, p =0.0032], with decreased pYp190 levels in both arg +/− Nex-β1 +/− and Nex-β1 −/− mice. Student's post-hoc t -test vs WT, P42 arg +/− Nex-β1 +/− p =0.002; P42 Nex-β1 −/− p =0.007, n = 4–8 mice for each genotype. f Quantification of Rho activity in arg +/− Nex-β1 +/− and Nex-β1 −/− mice. The amount of active Rho-GTP from each mutant mouse was compared to a WT littermate run in the same assay. Both arg +/− Nex-β1 +/− and Nex-β1 −/− mice had increased hippocampal Rho activity. One sample t -test comparing means to 1, P42 arg +/− Nex-β1 +/− p =0.02; P42 Nex-β1 −/− p =0.03. n =3–7 mice per group. Values represent mean ± SEM, * p

    Article Snippet: Coimmunoprecipitated proteins and loading controls were detected by stripping and reprobing the blot with anti-120RasGAP (Upstate) and anti-p190RhoGAP (BD Biosciences) antibodies.

    Techniques: Activity Assay, Mouse Assay, Immunoprecipitation, Mutagenesis

    Model for integrin β1-Arg signaling in dendritic spines (1) Integrin β1 is activated by binding to an unknown ligand. (2) The Arg kinase domain binds to the now exposed integrin β1 tail, stimulating Arg kinase activity by relieving autoinhibitory contacts between the SH3-SH2 domains and the kinase domain. (3) Arg phosphorylates p190RhoGAP, promoting its association with p120RasGAP at the membrane. (4) The p120RasGAP:p190R hoGAP complex inhibits RhoA, stabilizing synapses and dendrites.

    Journal: The Journal of Neuroscience

    Article Title: Integrin ?1 signals through Arg to regulate postnatal dendritic arborization, synapse density, and behavior

    doi: 10.1523/JNEUROSCI.3942-11.2012

    Figure Lengend Snippet: Model for integrin β1-Arg signaling in dendritic spines (1) Integrin β1 is activated by binding to an unknown ligand. (2) The Arg kinase domain binds to the now exposed integrin β1 tail, stimulating Arg kinase activity by relieving autoinhibitory contacts between the SH3-SH2 domains and the kinase domain. (3) Arg phosphorylates p190RhoGAP, promoting its association with p120RasGAP at the membrane. (4) The p120RasGAP:p190R hoGAP complex inhibits RhoA, stabilizing synapses and dendrites.

    Article Snippet: Coimmunoprecipitated proteins and loading controls were detected by stripping and reprobing the blot with anti-120RasGAP (Upstate) and anti-p190RhoGAP (BD Biosciences) antibodies.

    Techniques: Binding Assay, Activity Assay

    The cAMP target, Epac, suppresses EMT and cell migration in epicardial cell outgrowth from heart explants. A , Western blot showing that Epac1, p190RhoGAP, and Rap1 proteins were expressed in EMCs and the PE. B , the Epac activator, 007 (50 μ m ),

    Journal: The Journal of Biological Chemistry

    Article Title: Signaling Pathways That Control Rho Kinase Activity Maintain the Embryonic Epicardial Progenitor State

    doi: 10.1074/jbc.M114.613190

    Figure Lengend Snippet: The cAMP target, Epac, suppresses EMT and cell migration in epicardial cell outgrowth from heart explants. A , Western blot showing that Epac1, p190RhoGAP, and Rap1 proteins were expressed in EMCs and the PE. B , the Epac activator, 007 (50 μ m ),

    Article Snippet: The following antibodies were used: mouse monoclonal anti-MYPT1 (1:1000 Western blot (WB); BD Biosciences); anti-RhoA (1:500 WB; Santa Cruz Biotechnology Inc.); anti-Rnd3 (1:100 WB; Millipore); anti-Rnd3 (1:400 for immunolabeling; Santa Cruz Biotechnology Inc.); anti-p190RhoGAP (1:500 WB and 1:1500 for immunolabeling; BD Transduction Laboratories); anti-SMA (SM α-actin, 1:200 for immunolabeling and 1:1000 for WB; Sigma); GAPDH (1:5000 WB; Millipore); rabbit polyclonal anti-MYPT1 Thr-853 (1:500 WB; Millipore); anti-MYPT1 Thr-696 (1:500 WB; Millipore); anti-E-cadherin (1:200 immunolabeling/1:2000 WB: Zymed Laboratories Inc.); anti-SM22 (1:200 immunolabeling/1:5000 WB, Millipore); anti-ZO-1 (1:200 immunolabeling/1:1000 WB: Zymed Laboratories Inc.); anti-LARG (1:100 WB, Santa Cruz Biotechnology Inc.); anti-vinculin (1:500 immunolabeling; Sigma); p63RhoGEF (GEFT) (1:200 WB; Proteintech Group Inc.); anti-vimentin (1:200 WB immunolabeling/1:1000 WB; Sigma); anti-Rap1 (1:500; Santa Cruz Biotechnology Inc.); anti-GEF H1 (1:200 WB; Cell Signaling); goat polyclonal anti-Rnd1 (1:2000 WB, Santa Cruz Biotechnology Inc.; anti-Epac1 (1:500 WB; Abcam); anti-Epac 2 (1:200 WB; Santa Cruz Biotechnology Inc.).

    Techniques: Migration, Western Blot

    Rnd3 associates with p190RhoGAP in epicardial cells in the epithelial state; overall signaling scheme. A , anti-p190RhoGAP antibody, but not a nonspecific mouse IgG antibody, coimmunoprecipitated ( IP ) endogenous p190RhoGAP and Rnd 3. Anti-Rnd3 antibody,

    Journal: The Journal of Biological Chemistry

    Article Title: Signaling Pathways That Control Rho Kinase Activity Maintain the Embryonic Epicardial Progenitor State

    doi: 10.1074/jbc.M114.613190

    Figure Lengend Snippet: Rnd3 associates with p190RhoGAP in epicardial cells in the epithelial state; overall signaling scheme. A , anti-p190RhoGAP antibody, but not a nonspecific mouse IgG antibody, coimmunoprecipitated ( IP ) endogenous p190RhoGAP and Rnd 3. Anti-Rnd3 antibody,

    Article Snippet: The following antibodies were used: mouse monoclonal anti-MYPT1 (1:1000 Western blot (WB); BD Biosciences); anti-RhoA (1:500 WB; Santa Cruz Biotechnology Inc.); anti-Rnd3 (1:100 WB; Millipore); anti-Rnd3 (1:400 for immunolabeling; Santa Cruz Biotechnology Inc.); anti-p190RhoGAP (1:500 WB and 1:1500 for immunolabeling; BD Transduction Laboratories); anti-SMA (SM α-actin, 1:200 for immunolabeling and 1:1000 for WB; Sigma); GAPDH (1:5000 WB; Millipore); rabbit polyclonal anti-MYPT1 Thr-853 (1:500 WB; Millipore); anti-MYPT1 Thr-696 (1:500 WB; Millipore); anti-E-cadherin (1:200 immunolabeling/1:2000 WB: Zymed Laboratories Inc.); anti-SM22 (1:200 immunolabeling/1:5000 WB, Millipore); anti-ZO-1 (1:200 immunolabeling/1:1000 WB: Zymed Laboratories Inc.); anti-LARG (1:100 WB, Santa Cruz Biotechnology Inc.); anti-vinculin (1:500 immunolabeling; Sigma); p63RhoGEF (GEFT) (1:200 WB; Proteintech Group Inc.); anti-vimentin (1:200 WB immunolabeling/1:1000 WB; Sigma); anti-Rap1 (1:500; Santa Cruz Biotechnology Inc.); anti-GEF H1 (1:200 WB; Cell Signaling); goat polyclonal anti-Rnd1 (1:2000 WB, Santa Cruz Biotechnology Inc.; anti-Epac1 (1:500 WB; Abcam); anti-Epac 2 (1:200 WB; Santa Cruz Biotechnology Inc.).

    Techniques:

    DIP interacts with p190RhoGAP and Vav2 in podocytes. Lysates from control and Nef podocytes were immunoprecipitated ( IP ) with anti-DIP and immunoblotted ( IB ) with anti-p190 or anti-Vav2. DIP co-precipitates with both p190RhoGAP and Vav2 in both control

    Journal: The Journal of Biological Chemistry

    Article Title: HIV-1 Nef Disrupts the Podocyte Actin Cytoskeleton by Interacting with Diaphanous Interacting Protein *

    doi: 10.1074/jbc.M708920200

    Figure Lengend Snippet: DIP interacts with p190RhoGAP and Vav2 in podocytes. Lysates from control and Nef podocytes were immunoprecipitated ( IP ) with anti-DIP and immunoblotted ( IB ) with anti-p190 or anti-Vav2. DIP co-precipitates with both p190RhoGAP and Vav2 in both control

    Article Snippet: After extensive washing, precipitated proteins dissociated from the beads using 2× SDS-PAGE sample buffer, and Western blot analysis was performed with monoclonal anti-p190RhoGAP (BD Bioscience); polyclonal anti-DIP was made by Dr. Tominaga as previously described ( ).

    Techniques: Immunoprecipitation

    A , DIP dominant negative ( DN ) blocks Nef-induced p190RhoGAP phosphorylation. 293T cells were transfected with either control, Nef, or Nef P XX P along with either control or DIPΔPro. Control with EGF was used as a positive control. Pull-down

    Journal: The Journal of Biological Chemistry

    Article Title: HIV-1 Nef Disrupts the Podocyte Actin Cytoskeleton by Interacting with Diaphanous Interacting Protein *

    doi: 10.1074/jbc.M708920200

    Figure Lengend Snippet: A , DIP dominant negative ( DN ) blocks Nef-induced p190RhoGAP phosphorylation. 293T cells were transfected with either control, Nef, or Nef P XX P along with either control or DIPΔPro. Control with EGF was used as a positive control. Pull-down

    Article Snippet: After extensive washing, precipitated proteins dissociated from the beads using 2× SDS-PAGE sample buffer, and Western blot analysis was performed with monoclonal anti-p190RhoGAP (BD Bioscience); polyclonal anti-DIP was made by Dr. Tominaga as previously described ( ).

    Techniques: Dominant Negative Mutation, Transfection, Positive Control

    Nef interacts with Src, DIP, and Vav2. DIP interacts with both Nef and p190RhoAGAP, mediating Nef-Src-induced phosphorylation of p190RhoGAP. Nef interacts directly with Vav2 to induce Src-mediated Vav2 phosphorylation. The interaction between DIP and

    Journal: The Journal of Biological Chemistry

    Article Title: HIV-1 Nef Disrupts the Podocyte Actin Cytoskeleton by Interacting with Diaphanous Interacting Protein *

    doi: 10.1074/jbc.M708920200

    Figure Lengend Snippet: Nef interacts with Src, DIP, and Vav2. DIP interacts with both Nef and p190RhoAGAP, mediating Nef-Src-induced phosphorylation of p190RhoGAP. Nef interacts directly with Vav2 to induce Src-mediated Vav2 phosphorylation. The interaction between DIP and

    Article Snippet: After extensive washing, precipitated proteins dissociated from the beads using 2× SDS-PAGE sample buffer, and Western blot analysis was performed with monoclonal anti-p190RhoGAP (BD Bioscience); polyclonal anti-DIP was made by Dr. Tominaga as previously described ( ).

    Techniques: