Journal: Chinese Medicine

Article Title: Buyang Huanwu decoction ameliorates myocardial injury and attenuates platelet activation by regulating the PI3 kinase/Rap1/integrin α(IIb)β(3) pathway

doi: 10.1186/s13020-024-00976-0

Figure Lengend Snippet: BYHWD alleviated MI-induced platelet activation by downregulating Rap1 signaling. Quantitative real-time PCR analysis of Rap1, FRG, Src, CDC42, Akt, PI3K, and ACTG1. * P < 0.05 and ** P < 0.01 when compared to the sham group; # P < 0.05 and ## P < 0.01 when compared to the model group; Data are presented with mean ± SD; n = 6 in each group. Rap1: Ras-related protein Rap-1; FRG: ARH/RhoGEF and pleckstrin domain protein 2; Src: nonreceptor tyrosine kinase; CDC42: cell division cycle 42; Akt: AKT serine/threonine kinase; PI3K: phosphoinositide-3-kinase; ACTG1: actin gamma 1

Article Snippet: Membranes were firstly blocked with 5% BSA dissolved in Tris buffered saline (TBS) with Tween for a hour, and then probed with appropriate antibodies at 1:1000 against phosphoinositide-3-kinase (PI3K) (ab154598, Abcam, Cambridge, UK), phospho-phosphoinositide-3-kinase (p-PI3K) (341468, ZEN BIO, Chengdu, China), AKT serine/threonine kinase (Akt) (ab179463, Abcam, Cambridge, UK), phospho-Akt serine/threonine kinase (p-Akt) (ab38449, Abcam, Cambridge, UK), Rap1(#2399, CST, Boston, USA), cell division cycle 42 (CDC42) (ab187643, Abcam, Cambridge, UK), nonreceptor tyrosine kinase (Src) (2019S, CST, Boston, USA), phospho-nonreceptor tyrosine kinase (p-Src) (6943S, CST, Boston, USA), beta-actin (ab6276, Abcam, Cambridge, UK) at 4 ℃ with gentle agitation overnight.

Techniques: Activation Assay, Real-time Polymerase Chain Reaction