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Santa Cruz Biotechnology p neu
P Neu, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology rabbit anti p neu y1248
Rabbit Anti P Neu Y1248, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyclonal anti phospho her2 p neu  (Cell Signaling Technology Inc)


Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc polyclonal anti phospho her2 p neu
    Polyclonal Anti Phospho Her2 P Neu, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal anti phospho her2 p neu/product/Cell Signaling Technology Inc
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    Structured Review

    Santa Cruz Biotechnology p neu tyr 1248 r
    P Neu Tyr 1248 R, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Structured Review

    Santa Cruz Biotechnology poab anti phospho her2 p neu
    (A) Schematic representation of the MMTV-driven human Δ16HER2-LUC transgene, with the MMTV LTR promoter (pMMTV, red), the human Δ16HER2 cDNA (green), the IRES (internal ribosome entry site, yellow), the luciferase cDNA (LUC, purple), and the termination signal from the SV40 (Poly A, blue). Relevant restriction sites are indicated. (B) Graphic representation of murine chromosome 5 divided into A–G regions and numbered subregions, showing that the transgene integrates in region E-1. (C, D) An F2 female transgenic mouse with primary breast tumors (arrows) just before tumor removal. (E) Bioluminescence analysis of a 28-week-old tumor-bearing Δ16HER2-LUC transgenic mouse (founder female), using the in VivoVision Systems, Xenogen. (F and G) Immunohistochemical detection of <t>HER2</t> and luciferase, respectively, showing strong and uniform expression of both proteins in sections of a mammary tumor, while the normal duct on the right appears negative. Magnification: ×400.
    Poab Anti Phospho Her2 P Neu, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poab anti phospho her2 p neu/product/Santa Cruz Biotechnology
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    1) Product Images from "The Human Splice Variant Δ16HER2 Induces Rapid Tumor Onset in a Reporter Transgenic Mouse"

    Article Title: The Human Splice Variant Δ16HER2 Induces Rapid Tumor Onset in a Reporter Transgenic Mouse

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0018727

    (A) Schematic representation of the MMTV-driven human Δ16HER2-LUC transgene, with the MMTV LTR promoter (pMMTV, red), the human Δ16HER2 cDNA (green), the IRES (internal ribosome entry site, yellow), the luciferase cDNA (LUC, purple), and the termination signal from the SV40 (Poly A, blue). Relevant restriction sites are indicated. (B) Graphic representation of murine chromosome 5 divided into A–G regions and numbered subregions, showing that the transgene integrates in region E-1. (C, D) An F2 female transgenic mouse with primary breast tumors (arrows) just before tumor removal. (E) Bioluminescence analysis of a 28-week-old tumor-bearing Δ16HER2-LUC transgenic mouse (founder female), using the in VivoVision Systems, Xenogen. (F and G) Immunohistochemical detection of HER2 and luciferase, respectively, showing strong and uniform expression of both proteins in sections of a mammary tumor, while the normal duct on the right appears negative. Magnification: ×400.
    Figure Legend Snippet: (A) Schematic representation of the MMTV-driven human Δ16HER2-LUC transgene, with the MMTV LTR promoter (pMMTV, red), the human Δ16HER2 cDNA (green), the IRES (internal ribosome entry site, yellow), the luciferase cDNA (LUC, purple), and the termination signal from the SV40 (Poly A, blue). Relevant restriction sites are indicated. (B) Graphic representation of murine chromosome 5 divided into A–G regions and numbered subregions, showing that the transgene integrates in region E-1. (C, D) An F2 female transgenic mouse with primary breast tumors (arrows) just before tumor removal. (E) Bioluminescence analysis of a 28-week-old tumor-bearing Δ16HER2-LUC transgenic mouse (founder female), using the in VivoVision Systems, Xenogen. (F and G) Immunohistochemical detection of HER2 and luciferase, respectively, showing strong and uniform expression of both proteins in sections of a mammary tumor, while the normal duct on the right appears negative. Magnification: ×400.

    Techniques Used: Luciferase, Transgenic Assay, Immunohistochemical staining, Expressing

    (A) Protein extracts from 4 different Δ16HER2 tumor samples (lanes 1–4) and breast cancer cell lines BT474 (lane 5) and ZR-75.1 (lane 6) were separated by 4–12% gradient SDS-PAGE under non-reducing conditions and probed, respectively, with MAb Ab3 (HER2 M and D) and PoAb anti-phospho-HER2 p-neu (p-HER2 M and D). (B) The same protein extracts (lanes 1–6) were analyzed under reducing conditions for activation of the following HER2 downstream oncogenic signaling pathways: MAPK, AKT, Src and STAT3. Actin was used to normalize protein loading. (C) Proteins from the pool of whole Δ16HER2 tumor extracts (lanes 1, subpanels a and b) and purified plasma membrane extracts (lanes 2, subpanels a and b) were separated by 4–12% gradient SDS-PAGE under non-reducing conditions and probed with MAb Ab3 (a) and PoAb antiphospho-HER2 p-neu (b). E-cadherin was used as a plasma membrane marker.
    Figure Legend Snippet: (A) Protein extracts from 4 different Δ16HER2 tumor samples (lanes 1–4) and breast cancer cell lines BT474 (lane 5) and ZR-75.1 (lane 6) were separated by 4–12% gradient SDS-PAGE under non-reducing conditions and probed, respectively, with MAb Ab3 (HER2 M and D) and PoAb anti-phospho-HER2 p-neu (p-HER2 M and D). (B) The same protein extracts (lanes 1–6) were analyzed under reducing conditions for activation of the following HER2 downstream oncogenic signaling pathways: MAPK, AKT, Src and STAT3. Actin was used to normalize protein loading. (C) Proteins from the pool of whole Δ16HER2 tumor extracts (lanes 1, subpanels a and b) and purified plasma membrane extracts (lanes 2, subpanels a and b) were separated by 4–12% gradient SDS-PAGE under non-reducing conditions and probed with MAb Ab3 (a) and PoAb antiphospho-HER2 p-neu (b). E-cadherin was used as a plasma membrane marker.

    Techniques Used: SDS Page, Activation Assay, Purification, Marker

    (A) Bioluminescence analysis of an F2 female transgenic mouse at 14 weeks of age and (B) whole-mount analysis of the inguinal mammary glands; arrows indicate non-palpable tumors. (C) Hematoxylin-eosin and immunohistochemical staining for HER2 (D) and PCNA (E) of non-palpable mammary tumors at 14 weeks. Magnification: B, ×6; C–E, ×400.
    Figure Legend Snippet: (A) Bioluminescence analysis of an F2 female transgenic mouse at 14 weeks of age and (B) whole-mount analysis of the inguinal mammary glands; arrows indicate non-palpable tumors. (C) Hematoxylin-eosin and immunohistochemical staining for HER2 (D) and PCNA (E) of non-palpable mammary tumors at 14 weeks. Magnification: B, ×6; C–E, ×400.

    Techniques Used: Transgenic Assay, Immunohistochemical staining, Staining


    Structured Review

    Santa Cruz Biotechnology anti p neu tyr 1248 r rabbit polyclonal antibody
    Anti P Neu Tyr 1248 R Rabbit Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p neu  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc p neu
    P Neu, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p neu/product/Cell Signaling Technology Inc
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    Santa Cruz Biotechnology anti phospho her 2
    Anti Phospho Her 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology p neu
    P Neu, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p neu/product/Santa Cruz Biotechnology
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    p neu - by Bioz Stars, 2024-07
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    Structured Review

    Santa Cruz Biotechnology p neu
    P Neu, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p neu/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p neu - by Bioz Stars, 2024-07
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    Santa Cruz Biotechnology p neu
    P Neu, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc polyclonal anti phospho her2 p neu
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    Santa Cruz Biotechnology poab anti phospho her2 p neu
    (A) Schematic representation of the MMTV-driven human Δ16HER2-LUC transgene, with the MMTV LTR promoter (pMMTV, red), the human Δ16HER2 cDNA (green), the IRES (internal ribosome entry site, yellow), the luciferase cDNA (LUC, purple), and the termination signal from the SV40 (Poly A, blue). Relevant restriction sites are indicated. (B) Graphic representation of murine chromosome 5 divided into A–G regions and numbered subregions, showing that the transgene integrates in region E-1. (C, D) An F2 female transgenic mouse with primary breast tumors (arrows) just before tumor removal. (E) Bioluminescence analysis of a 28-week-old tumor-bearing Δ16HER2-LUC transgenic mouse (founder female), using the in VivoVision Systems, Xenogen. (F and G) Immunohistochemical detection of <t>HER2</t> and luciferase, respectively, showing strong and uniform expression of both proteins in sections of a mammary tumor, while the normal duct on the right appears negative. Magnification: ×400.
    Poab Anti Phospho Her2 P Neu, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti p neu tyr 1248 r rabbit polyclonal antibody
    (A) Schematic representation of the MMTV-driven human Δ16HER2-LUC transgene, with the MMTV LTR promoter (pMMTV, red), the human Δ16HER2 cDNA (green), the IRES (internal ribosome entry site, yellow), the luciferase cDNA (LUC, purple), and the termination signal from the SV40 (Poly A, blue). Relevant restriction sites are indicated. (B) Graphic representation of murine chromosome 5 divided into A–G regions and numbered subregions, showing that the transgene integrates in region E-1. (C, D) An F2 female transgenic mouse with primary breast tumors (arrows) just before tumor removal. (E) Bioluminescence analysis of a 28-week-old tumor-bearing Δ16HER2-LUC transgenic mouse (founder female), using the in VivoVision Systems, Xenogen. (F and G) Immunohistochemical detection of <t>HER2</t> and luciferase, respectively, showing strong and uniform expression of both proteins in sections of a mammary tumor, while the normal duct on the right appears negative. Magnification: ×400.
    Anti P Neu Tyr 1248 R Rabbit Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc p neu
    (A) Schematic representation of the MMTV-driven human Δ16HER2-LUC transgene, with the MMTV LTR promoter (pMMTV, red), the human Δ16HER2 cDNA (green), the IRES (internal ribosome entry site, yellow), the luciferase cDNA (LUC, purple), and the termination signal from the SV40 (Poly A, blue). Relevant restriction sites are indicated. (B) Graphic representation of murine chromosome 5 divided into A–G regions and numbered subregions, showing that the transgene integrates in region E-1. (C, D) An F2 female transgenic mouse with primary breast tumors (arrows) just before tumor removal. (E) Bioluminescence analysis of a 28-week-old tumor-bearing Δ16HER2-LUC transgenic mouse (founder female), using the in VivoVision Systems, Xenogen. (F and G) Immunohistochemical detection of <t>HER2</t> and luciferase, respectively, showing strong and uniform expression of both proteins in sections of a mammary tumor, while the normal duct on the right appears negative. Magnification: ×400.
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    Santa Cruz Biotechnology anti phospho her 2
    (A) Schematic representation of the MMTV-driven human Δ16HER2-LUC transgene, with the MMTV LTR promoter (pMMTV, red), the human Δ16HER2 cDNA (green), the IRES (internal ribosome entry site, yellow), the luciferase cDNA (LUC, purple), and the termination signal from the SV40 (Poly A, blue). Relevant restriction sites are indicated. (B) Graphic representation of murine chromosome 5 divided into A–G regions and numbered subregions, showing that the transgene integrates in region E-1. (C, D) An F2 female transgenic mouse with primary breast tumors (arrows) just before tumor removal. (E) Bioluminescence analysis of a 28-week-old tumor-bearing Δ16HER2-LUC transgenic mouse (founder female), using the in VivoVision Systems, Xenogen. (F and G) Immunohistochemical detection of <t>HER2</t> and luciferase, respectively, showing strong and uniform expression of both proteins in sections of a mammary tumor, while the normal duct on the right appears negative. Magnification: ×400.
    Anti Phospho Her 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) Schematic representation of the MMTV-driven human Δ16HER2-LUC transgene, with the MMTV LTR promoter (pMMTV, red), the human Δ16HER2 cDNA (green), the IRES (internal ribosome entry site, yellow), the luciferase cDNA (LUC, purple), and the termination signal from the SV40 (Poly A, blue). Relevant restriction sites are indicated. (B) Graphic representation of murine chromosome 5 divided into A–G regions and numbered subregions, showing that the transgene integrates in region E-1. (C, D) An F2 female transgenic mouse with primary breast tumors (arrows) just before tumor removal. (E) Bioluminescence analysis of a 28-week-old tumor-bearing Δ16HER2-LUC transgenic mouse (founder female), using the in VivoVision Systems, Xenogen. (F and G) Immunohistochemical detection of HER2 and luciferase, respectively, showing strong and uniform expression of both proteins in sections of a mammary tumor, while the normal duct on the right appears negative. Magnification: ×400.

    Journal: PLoS ONE

    Article Title: The Human Splice Variant Δ16HER2 Induces Rapid Tumor Onset in a Reporter Transgenic Mouse

    doi: 10.1371/journal.pone.0018727

    Figure Lengend Snippet: (A) Schematic representation of the MMTV-driven human Δ16HER2-LUC transgene, with the MMTV LTR promoter (pMMTV, red), the human Δ16HER2 cDNA (green), the IRES (internal ribosome entry site, yellow), the luciferase cDNA (LUC, purple), and the termination signal from the SV40 (Poly A, blue). Relevant restriction sites are indicated. (B) Graphic representation of murine chromosome 5 divided into A–G regions and numbered subregions, showing that the transgene integrates in region E-1. (C, D) An F2 female transgenic mouse with primary breast tumors (arrows) just before tumor removal. (E) Bioluminescence analysis of a 28-week-old tumor-bearing Δ16HER2-LUC transgenic mouse (founder female), using the in VivoVision Systems, Xenogen. (F and G) Immunohistochemical detection of HER2 and luciferase, respectively, showing strong and uniform expression of both proteins in sections of a mammary tumor, while the normal duct on the right appears negative. Magnification: ×400.

    Article Snippet: Membranes were probed with mouse monoclonal antibodies (MAbs) Ab3 (1 µg/ml; Calbiochem, Darmstadt, Germany), anti-Src (1∶1000; Cell Signaling Technology, Beverly, MA), anti-phospho-Stat3 (1∶1000; Santa Cruz Biotechnology, Santa Cruz, CA), anti-actin AC-40 (0.4 mg/ml; Sigma), PoAb anti-phospho-HER2 p-Neu (tyr 1248; 0.5 mg/ml, Santa Cruz Biotechnology), anti-phospho-p44/42 MAPK (Erk1/2; 1∶2000; Cell Signaling Technology), anti-p44/42 MAPK (Erk1/2; 1∶1.000; Cell Signaling Technology), anti-phospho-Akt (Ser 473; 1∶1000; Cell Signaling Technology), anti-Akt (1∶1000; Cell Signaling Technology), anti-phospho-Src (Tyr 416; 1∶1000; Cell Signaling Technology), anti-Stat3 (1∶1000; Santa Cruz Biotechnology) and anti-E-cadherin (#4065, 1∶1000; Cell Signaling Technology).

    Techniques: Luciferase, Transgenic Assay, Immunohistochemical staining, Expressing

    (A) Protein extracts from 4 different Δ16HER2 tumor samples (lanes 1–4) and breast cancer cell lines BT474 (lane 5) and ZR-75.1 (lane 6) were separated by 4–12% gradient SDS-PAGE under non-reducing conditions and probed, respectively, with MAb Ab3 (HER2 M and D) and PoAb anti-phospho-HER2 p-neu (p-HER2 M and D). (B) The same protein extracts (lanes 1–6) were analyzed under reducing conditions for activation of the following HER2 downstream oncogenic signaling pathways: MAPK, AKT, Src and STAT3. Actin was used to normalize protein loading. (C) Proteins from the pool of whole Δ16HER2 tumor extracts (lanes 1, subpanels a and b) and purified plasma membrane extracts (lanes 2, subpanels a and b) were separated by 4–12% gradient SDS-PAGE under non-reducing conditions and probed with MAb Ab3 (a) and PoAb antiphospho-HER2 p-neu (b). E-cadherin was used as a plasma membrane marker.

    Journal: PLoS ONE

    Article Title: The Human Splice Variant Δ16HER2 Induces Rapid Tumor Onset in a Reporter Transgenic Mouse

    doi: 10.1371/journal.pone.0018727

    Figure Lengend Snippet: (A) Protein extracts from 4 different Δ16HER2 tumor samples (lanes 1–4) and breast cancer cell lines BT474 (lane 5) and ZR-75.1 (lane 6) were separated by 4–12% gradient SDS-PAGE under non-reducing conditions and probed, respectively, with MAb Ab3 (HER2 M and D) and PoAb anti-phospho-HER2 p-neu (p-HER2 M and D). (B) The same protein extracts (lanes 1–6) were analyzed under reducing conditions for activation of the following HER2 downstream oncogenic signaling pathways: MAPK, AKT, Src and STAT3. Actin was used to normalize protein loading. (C) Proteins from the pool of whole Δ16HER2 tumor extracts (lanes 1, subpanels a and b) and purified plasma membrane extracts (lanes 2, subpanels a and b) were separated by 4–12% gradient SDS-PAGE under non-reducing conditions and probed with MAb Ab3 (a) and PoAb antiphospho-HER2 p-neu (b). E-cadherin was used as a plasma membrane marker.

    Article Snippet: Membranes were probed with mouse monoclonal antibodies (MAbs) Ab3 (1 µg/ml; Calbiochem, Darmstadt, Germany), anti-Src (1∶1000; Cell Signaling Technology, Beverly, MA), anti-phospho-Stat3 (1∶1000; Santa Cruz Biotechnology, Santa Cruz, CA), anti-actin AC-40 (0.4 mg/ml; Sigma), PoAb anti-phospho-HER2 p-Neu (tyr 1248; 0.5 mg/ml, Santa Cruz Biotechnology), anti-phospho-p44/42 MAPK (Erk1/2; 1∶2000; Cell Signaling Technology), anti-p44/42 MAPK (Erk1/2; 1∶1.000; Cell Signaling Technology), anti-phospho-Akt (Ser 473; 1∶1000; Cell Signaling Technology), anti-Akt (1∶1000; Cell Signaling Technology), anti-phospho-Src (Tyr 416; 1∶1000; Cell Signaling Technology), anti-Stat3 (1∶1000; Santa Cruz Biotechnology) and anti-E-cadherin (#4065, 1∶1000; Cell Signaling Technology).

    Techniques: SDS Page, Activation Assay, Purification, Marker

    (A) Bioluminescence analysis of an F2 female transgenic mouse at 14 weeks of age and (B) whole-mount analysis of the inguinal mammary glands; arrows indicate non-palpable tumors. (C) Hematoxylin-eosin and immunohistochemical staining for HER2 (D) and PCNA (E) of non-palpable mammary tumors at 14 weeks. Magnification: B, ×6; C–E, ×400.

    Journal: PLoS ONE

    Article Title: The Human Splice Variant Δ16HER2 Induces Rapid Tumor Onset in a Reporter Transgenic Mouse

    doi: 10.1371/journal.pone.0018727

    Figure Lengend Snippet: (A) Bioluminescence analysis of an F2 female transgenic mouse at 14 weeks of age and (B) whole-mount analysis of the inguinal mammary glands; arrows indicate non-palpable tumors. (C) Hematoxylin-eosin and immunohistochemical staining for HER2 (D) and PCNA (E) of non-palpable mammary tumors at 14 weeks. Magnification: B, ×6; C–E, ×400.

    Article Snippet: Membranes were probed with mouse monoclonal antibodies (MAbs) Ab3 (1 µg/ml; Calbiochem, Darmstadt, Germany), anti-Src (1∶1000; Cell Signaling Technology, Beverly, MA), anti-phospho-Stat3 (1∶1000; Santa Cruz Biotechnology, Santa Cruz, CA), anti-actin AC-40 (0.4 mg/ml; Sigma), PoAb anti-phospho-HER2 p-Neu (tyr 1248; 0.5 mg/ml, Santa Cruz Biotechnology), anti-phospho-p44/42 MAPK (Erk1/2; 1∶2000; Cell Signaling Technology), anti-p44/42 MAPK (Erk1/2; 1∶1.000; Cell Signaling Technology), anti-phospho-Akt (Ser 473; 1∶1000; Cell Signaling Technology), anti-Akt (1∶1000; Cell Signaling Technology), anti-phospho-Src (Tyr 416; 1∶1000; Cell Signaling Technology), anti-Stat3 (1∶1000; Santa Cruz Biotechnology) and anti-E-cadherin (#4065, 1∶1000; Cell Signaling Technology).

    Techniques: Transgenic Assay, Immunohistochemical staining, Staining