p ampk  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p ampk
    P Ampk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phosphorylated p ampk  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phosphorylated p ampk
    gCTRP3 activates the <t>AMPK/SIRT1/Nrf2</t> signaling pathway. Expression levels of proteins associated with the AMPK/SIRT1/Nrf2 signaling pathway were determined in MC3T3-E1 cells following treatment with different concentrations of gCTRP3. *P<0.05 and ***P<0.001 vs. Control group. gCTRP3, globular C1q/tumor necrosis factor-related protein 3; p-, <t>phosphorylated;</t> AMPK, <t>AMP-activated</t> <t>protein</t> <t>kinase;</t> SIRT1, sirtuin 1; Nrf2, nuclear factor E2-related factor 2.
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    1) Product Images from "gCTRP3 inhibits oophorectomy‑induced osteoporosis by activating the AMPK/SIRT1/Nrf2 signaling pathway in mice"

    Article Title: gCTRP3 inhibits oophorectomy‑induced osteoporosis by activating the AMPK/SIRT1/Nrf2 signaling pathway in mice

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2024.13257

    gCTRP3 activates the AMPK/SIRT1/Nrf2 signaling pathway. Expression levels of proteins associated with the AMPK/SIRT1/Nrf2 signaling pathway were determined in MC3T3-E1 cells following treatment with different concentrations of gCTRP3. *P<0.05 and ***P<0.001 vs. Control group. gCTRP3, globular C1q/tumor necrosis factor-related protein 3; p-, phosphorylated; AMPK, AMP-activated protein kinase; SIRT1, sirtuin 1; Nrf2, nuclear factor E2-related factor 2.
    Figure Legend Snippet: gCTRP3 activates the AMPK/SIRT1/Nrf2 signaling pathway. Expression levels of proteins associated with the AMPK/SIRT1/Nrf2 signaling pathway were determined in MC3T3-E1 cells following treatment with different concentrations of gCTRP3. *P<0.05 and ***P<0.001 vs. Control group. gCTRP3, globular C1q/tumor necrosis factor-related protein 3; p-, phosphorylated; AMPK, AMP-activated protein kinase; SIRT1, sirtuin 1; Nrf2, nuclear factor E2-related factor 2.

    Techniques Used: Expressing

    gCTRP3 promotes the osteogenic differentiation of MC3T3-E1 cells through the AMP-activated protein kinase/sirtuin 1/nuclear factor E2-related factor 2 signaling pathway. (A) ALP activity of MC3T3-E1 cells was detected using an ALP assay kit following treatment with gCTRP3 and a signaling pathway inhibitor. (B) Osteoblast differentiation of MC3T3-E1 cells was determined using ALP staining following treatment with gCTRP3 and a signaling pathway inhibitor. (C) Calcium deposits in MC3T3-E1 cells were determined using ARS staining following treatment with gCTRP3 and a signaling pathway inhibitor. (D) Expression levels of osteogenic marker genes were determined using reverse transcription-quantitative PCR following treatment with gCTRP3 and a signaling pathway inhibitor. ***P<0.001 vs. Control group; # P<0.05, ## P<0.01 and ### P<0.001 vs. gCTRP3 group. gCTRP3, globular C1q/tumor necrosis factor-related protein 3; ALP, alkaline phosphatase; ARS, Alizarin red staining; RUNX2, Runt-related transcription factor-2; OCN, osteocalcin.
    Figure Legend Snippet: gCTRP3 promotes the osteogenic differentiation of MC3T3-E1 cells through the AMP-activated protein kinase/sirtuin 1/nuclear factor E2-related factor 2 signaling pathway. (A) ALP activity of MC3T3-E1 cells was detected using an ALP assay kit following treatment with gCTRP3 and a signaling pathway inhibitor. (B) Osteoblast differentiation of MC3T3-E1 cells was determined using ALP staining following treatment with gCTRP3 and a signaling pathway inhibitor. (C) Calcium deposits in MC3T3-E1 cells were determined using ARS staining following treatment with gCTRP3 and a signaling pathway inhibitor. (D) Expression levels of osteogenic marker genes were determined using reverse transcription-quantitative PCR following treatment with gCTRP3 and a signaling pathway inhibitor. ***P<0.001 vs. Control group; # P<0.05, ## P<0.01 and ### P<0.001 vs. gCTRP3 group. gCTRP3, globular C1q/tumor necrosis factor-related protein 3; ALP, alkaline phosphatase; ARS, Alizarin red staining; RUNX2, Runt-related transcription factor-2; OCN, osteocalcin.

    Techniques Used: Activity Assay, ALP Assay, Staining, Expressing, Marker, Reverse Transcription, Real-time Polymerase Chain Reaction

    p ampk cat no 2535 rrid ab 331250  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc p ampk cat no 2535 rrid ab 331250
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    anti p ampk thr172 2535s  (Cell Signaling Technology Inc)


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    p ampk 50081s  (Cell Signaling Technology Inc)


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    p ampk thr 172  (Cell Signaling Technology Inc)


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    gCTRP3 activates the <t>AMPK/SIRT1/Nrf2</t> signaling pathway. Expression levels of proteins associated with the AMPK/SIRT1/Nrf2 signaling pathway were determined in MC3T3-E1 cells following treatment with different concentrations of gCTRP3. *P<0.05 and ***P<0.001 vs. Control group. gCTRP3, globular C1q/tumor necrosis factor-related protein 3; p-, <t>phosphorylated;</t> AMPK, <t>AMP-activated</t> <t>protein</t> <t>kinase;</t> SIRT1, sirtuin 1; Nrf2, nuclear factor E2-related factor 2.
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    gCTRP3 activates the <t>AMPK/SIRT1/Nrf2</t> signaling pathway. Expression levels of proteins associated with the AMPK/SIRT1/Nrf2 signaling pathway were determined in MC3T3-E1 cells following treatment with different concentrations of gCTRP3. *P<0.05 and ***P<0.001 vs. Control group. gCTRP3, globular C1q/tumor necrosis factor-related protein 3; p-, <t>phosphorylated;</t> AMPK, <t>AMP-activated</t> <t>protein</t> <t>kinase;</t> SIRT1, sirtuin 1; Nrf2, nuclear factor E2-related factor 2.
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    gCTRP3 activates the AMPK/SIRT1/Nrf2 signaling pathway. Expression levels of proteins associated with the AMPK/SIRT1/Nrf2 signaling pathway were determined in MC3T3-E1 cells following treatment with different concentrations of gCTRP3. *P<0.05 and ***P<0.001 vs. Control group. gCTRP3, globular C1q/tumor necrosis factor-related protein 3; p-, phosphorylated; AMPK, AMP-activated protein kinase; SIRT1, sirtuin 1; Nrf2, nuclear factor E2-related factor 2.

    Journal: Molecular Medicine Reports

    Article Title: gCTRP3 inhibits oophorectomy‑induced osteoporosis by activating the AMPK/SIRT1/Nrf2 signaling pathway in mice

    doi: 10.3892/mmr.2024.13257

    Figure Lengend Snippet: gCTRP3 activates the AMPK/SIRT1/Nrf2 signaling pathway. Expression levels of proteins associated with the AMPK/SIRT1/Nrf2 signaling pathway were determined in MC3T3-E1 cells following treatment with different concentrations of gCTRP3. *P<0.05 and ***P<0.001 vs. Control group. gCTRP3, globular C1q/tumor necrosis factor-related protein 3; p-, phosphorylated; AMPK, AMP-activated protein kinase; SIRT1, sirtuin 1; Nrf2, nuclear factor E2-related factor 2.

    Article Snippet: After blocking with 5% BSA for 1 h at room temperature, the membranes were incubated with primary antibodies against phosphorylated (p)-AMPK (1:1,000 dilution; cat. no. 2535T; Cell Signaling Technology, Inc.), AMPK (1:1,000; cat. no. 2532S; Cell Signaling Technology, Inc.), SIRT1 (1:1,000; cat. no. 9475T; Cell Signaling Technology, Inc.), Nrf2 (1:1,000; cat. no. 80593-1-RR; Proteintech Group, Inc.) and GAPDH (1:1,000; cat. no. 2118T; Cell Signaling Technology, Inc.) overnight at 4°C.

    Techniques: Expressing

    gCTRP3 promotes the osteogenic differentiation of MC3T3-E1 cells through the AMP-activated protein kinase/sirtuin 1/nuclear factor E2-related factor 2 signaling pathway. (A) ALP activity of MC3T3-E1 cells was detected using an ALP assay kit following treatment with gCTRP3 and a signaling pathway inhibitor. (B) Osteoblast differentiation of MC3T3-E1 cells was determined using ALP staining following treatment with gCTRP3 and a signaling pathway inhibitor. (C) Calcium deposits in MC3T3-E1 cells were determined using ARS staining following treatment with gCTRP3 and a signaling pathway inhibitor. (D) Expression levels of osteogenic marker genes were determined using reverse transcription-quantitative PCR following treatment with gCTRP3 and a signaling pathway inhibitor. ***P<0.001 vs. Control group; # P<0.05, ## P<0.01 and ### P<0.001 vs. gCTRP3 group. gCTRP3, globular C1q/tumor necrosis factor-related protein 3; ALP, alkaline phosphatase; ARS, Alizarin red staining; RUNX2, Runt-related transcription factor-2; OCN, osteocalcin.

    Journal: Molecular Medicine Reports

    Article Title: gCTRP3 inhibits oophorectomy‑induced osteoporosis by activating the AMPK/SIRT1/Nrf2 signaling pathway in mice

    doi: 10.3892/mmr.2024.13257

    Figure Lengend Snippet: gCTRP3 promotes the osteogenic differentiation of MC3T3-E1 cells through the AMP-activated protein kinase/sirtuin 1/nuclear factor E2-related factor 2 signaling pathway. (A) ALP activity of MC3T3-E1 cells was detected using an ALP assay kit following treatment with gCTRP3 and a signaling pathway inhibitor. (B) Osteoblast differentiation of MC3T3-E1 cells was determined using ALP staining following treatment with gCTRP3 and a signaling pathway inhibitor. (C) Calcium deposits in MC3T3-E1 cells were determined using ARS staining following treatment with gCTRP3 and a signaling pathway inhibitor. (D) Expression levels of osteogenic marker genes were determined using reverse transcription-quantitative PCR following treatment with gCTRP3 and a signaling pathway inhibitor. ***P<0.001 vs. Control group; # P<0.05, ## P<0.01 and ### P<0.001 vs. gCTRP3 group. gCTRP3, globular C1q/tumor necrosis factor-related protein 3; ALP, alkaline phosphatase; ARS, Alizarin red staining; RUNX2, Runt-related transcription factor-2; OCN, osteocalcin.

    Article Snippet: After blocking with 5% BSA for 1 h at room temperature, the membranes were incubated with primary antibodies against phosphorylated (p)-AMPK (1:1,000 dilution; cat. no. 2535T; Cell Signaling Technology, Inc.), AMPK (1:1,000; cat. no. 2532S; Cell Signaling Technology, Inc.), SIRT1 (1:1,000; cat. no. 9475T; Cell Signaling Technology, Inc.), Nrf2 (1:1,000; cat. no. 80593-1-RR; Proteintech Group, Inc.) and GAPDH (1:1,000; cat. no. 2118T; Cell Signaling Technology, Inc.) overnight at 4°C.

    Techniques: Activity Assay, ALP Assay, Staining, Expressing, Marker, Reverse Transcription, Real-time Polymerase Chain Reaction