Structured Review

Proteintech nox2
Niclosamide inhibits PCM induced ROS generation in VICs. (A) Representative fluorescence microscopy images of DCFDA and DHE in live-adherent VICs with corresponding DAPI staining. (B) Relative level of ROS in VICs determined by microplate assay and were calculated as a percentage of CM. Results are expressed as mean ± SEM (n=3). *** p <0.001 vs. CM; # p <0.05; ## p <0.01 vs. PCM. (C) Relative level of NADPH oxidase in VICs was determined by lucigenin chemiluminiscence assay and chemiluminiscence intensities were calculated as a percentage of CM. Results are expressed as mean ± SEM (n=3). ** p <0.01 vs. CM; # p <0.05; ## p <0.01; ### p <0.001 vs. PCM. (D, E) The gene expression of <t>NOX2</t> and its catalytic subunit p22 phox were determined by RT-qPCR. Results are expressed as mean ± SEM (n=4). ** p <0.01; *** p <0.001 vs. CM; # p <0.05; ## p <0.01; ### p <0.001 vs. PCM. (F, G) The protein expression of Nox2 and p22 phox were determined by western blotting and quantification analysis. Results are expressed as mean ± SEM (n=4). * p <0.05; ** p <0.01; vs. CM; # p <0.05; ## p <0.01 vs. PCM.
Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Niclosamide Inhibits Aortic Valve Interstitial Cell Calcification by Interfering with the GSK-3β/β-Catenin Signaling Pathway"

Article Title: Niclosamide Inhibits Aortic Valve Interstitial Cell Calcification by Interfering with the GSK-3β/β-Catenin Signaling Pathway

Journal: Biomolecules & Therapeutics

doi: 10.4062/biomolther.2022.146

Niclosamide inhibits PCM induced ROS generation in VICs. (A) Representative fluorescence microscopy images of DCFDA and DHE in live-adherent VICs with corresponding DAPI staining. (B) Relative level of ROS in VICs determined by microplate assay and were calculated as a percentage of CM. Results are expressed as mean ± SEM (n=3). *** p <0.001 vs. CM; # p <0.05; ## p <0.01 vs. PCM. (C) Relative level of NADPH oxidase in VICs was determined by lucigenin chemiluminiscence assay and chemiluminiscence intensities were calculated as a percentage of CM. Results are expressed as mean ± SEM (n=3). ** p <0.01 vs. CM; # p <0.05; ## p <0.01; ### p <0.001 vs. PCM. (D, E) The gene expression of NOX2 and its catalytic subunit p22 phox were determined by RT-qPCR. Results are expressed as mean ± SEM (n=4). ** p <0.01; *** p <0.001 vs. CM; # p <0.05; ## p <0.01; ### p <0.001 vs. PCM. (F, G) The protein expression of Nox2 and p22 phox were determined by western blotting and quantification analysis. Results are expressed as mean ± SEM (n=4). * p <0.05; ** p <0.01; vs. CM; # p <0.05; ## p <0.01 vs. PCM.
Figure Legend Snippet: Niclosamide inhibits PCM induced ROS generation in VICs. (A) Representative fluorescence microscopy images of DCFDA and DHE in live-adherent VICs with corresponding DAPI staining. (B) Relative level of ROS in VICs determined by microplate assay and were calculated as a percentage of CM. Results are expressed as mean ± SEM (n=3). *** p <0.001 vs. CM; # p <0.05; ## p <0.01 vs. PCM. (C) Relative level of NADPH oxidase in VICs was determined by lucigenin chemiluminiscence assay and chemiluminiscence intensities were calculated as a percentage of CM. Results are expressed as mean ± SEM (n=3). ** p <0.01 vs. CM; # p <0.05; ## p <0.01; ### p <0.001 vs. PCM. (D, E) The gene expression of NOX2 and its catalytic subunit p22 phox were determined by RT-qPCR. Results are expressed as mean ± SEM (n=4). ** p <0.01; *** p <0.001 vs. CM; # p <0.05; ## p <0.01; ### p <0.001 vs. PCM. (F, G) The protein expression of Nox2 and p22 phox were determined by western blotting and quantification analysis. Results are expressed as mean ± SEM (n=4). * p <0.05; ** p <0.01; vs. CM; # p <0.05; ## p <0.01 vs. PCM.

Techniques Used: Fluorescence, Microscopy, Staining, Expressing, Quantitative RT-PCR, Western Blot


Structured Review

Proteintech nox2
Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
nox2 - by Bioz Stars, 2023-10
86/100 stars

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Structured Review

Proteintech anti nox2
Primer sequences for RT-qPCR.
Anti Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti nox2/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti nox2 - by Bioz Stars, 2023-10
86/100 stars

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1) Product Images from "Glucocorticoid-induced activation of NOX/ROS/NF-κB signaling in MSCs contributes to the development of GONFH"

Article Title: Glucocorticoid-induced activation of NOX/ROS/NF-κB signaling in MSCs contributes to the development of GONFH

Journal: Apoptosis

doi: 10.1007/s10495-023-01860-2

Primer sequences for RT-qPCR.
Figure Legend Snippet: Primer sequences for RT-qPCR.

Techniques Used: Sequencing

GCs-induced exacerbation of the OS microenvironment in the necrotic region of GONFH, with enhanced NOX protein expressions and ROS levels. ( A ) ROS staining and quantitative analysis of human femoral head. ( B ) ROS staining and quantitative analysis of rat femoral head. ( C ) ROS staining and quantitative analysis of MSCs treated with 1µM DEX. ( D ) Immunohistochemistry staining and quantitative analysis of NOX2 and NOX4 of human femoral head. ( E ) Immunohistochemistry staining and quantitative analysis of NOX2 and NOX4 of rat femoral head. ( F ) NOX2 and NOX4 mRNA of MSCs treated with 1µM DEX. ( G ) NOX2 and NOX4 protein levels of MSCs treated with 1µM DEX. PC, positive control, *** P < 0.001 (vs. GONFH or DEX).
Figure Legend Snippet: GCs-induced exacerbation of the OS microenvironment in the necrotic region of GONFH, with enhanced NOX protein expressions and ROS levels. ( A ) ROS staining and quantitative analysis of human femoral head. ( B ) ROS staining and quantitative analysis of rat femoral head. ( C ) ROS staining and quantitative analysis of MSCs treated with 1µM DEX. ( D ) Immunohistochemistry staining and quantitative analysis of NOX2 and NOX4 of human femoral head. ( E ) Immunohistochemistry staining and quantitative analysis of NOX2 and NOX4 of rat femoral head. ( F ) NOX2 and NOX4 mRNA of MSCs treated with 1µM DEX. ( G ) NOX2 and NOX4 protein levels of MSCs treated with 1µM DEX. PC, positive control, *** P < 0.001 (vs. GONFH or DEX).

Techniques Used: Staining, Immunohistochemistry, Positive Control

DPI counteracted the OS-promoting effects of DEX and reversed apoptosis and imbalance in osteogenic/lipogenic differentiation of MSCs. ( A ) ROS staining and quantitative analysis of MSCs treated with DEX and DPI. ( B ) NOX2, NOX4, CASP3, OSX and PPARγ mRNA of MSCs treated with DEX and DPI. ( C ) NOX2, NOX4, CASP3, OSX and PPARγ protein levels of MSCs treated with DEX and DPI. ( D ) Tunel staining and flow cytometry of MSCs treated with DEX and DPI. ( E ) ALP and Oil red O staining of MSCs treated with DEX and DPI for 7 or 14 days. PC, positive control, * P < 0.05, ** P < 0.01, *** P < 0.001 (vs. DEX).
Figure Legend Snippet: DPI counteracted the OS-promoting effects of DEX and reversed apoptosis and imbalance in osteogenic/lipogenic differentiation of MSCs. ( A ) ROS staining and quantitative analysis of MSCs treated with DEX and DPI. ( B ) NOX2, NOX4, CASP3, OSX and PPARγ mRNA of MSCs treated with DEX and DPI. ( C ) NOX2, NOX4, CASP3, OSX and PPARγ protein levels of MSCs treated with DEX and DPI. ( D ) Tunel staining and flow cytometry of MSCs treated with DEX and DPI. ( E ) ALP and Oil red O staining of MSCs treated with DEX and DPI for 7 or 14 days. PC, positive control, * P < 0.05, ** P < 0.01, *** P < 0.001 (vs. DEX).

Techniques Used: Staining, TUNEL Assay, Flow Cytometry, Positive Control

BAY partially reversed DEX-induced apoptosis and imbalance of osteogenic/lipogenic differentiation of MSCs. ( A ) Immunohistochemistry staining and quantitative analysis of p-p65 of rat femoral head. ( B ) NOX2, NOX4, CASP3, OSX and PPARγ mRNA of MSCs treated with DEX and BAY 11-7082. ( C ) CASP3, OSX and PPARγ protein levels of MSCs treated with DEX and BAY 11-7082. ( D ) ROS staining and quantitative analysis of MSCs treated with DEX and BAY 11-7082. ( E ) Tunel staining and flow cytometry of MSCs treated with DEX and BAY 11-7082. ( F ) ALP and Oil red O staining of MSCs treated with DEX and BAY 11-7082 for 7 or 14 days. PC, positive control, NS, no significance, * P < 0.05, ** P < 0.01, *** P < 0.001 (vs. GONFH or DEX).
Figure Legend Snippet: BAY partially reversed DEX-induced apoptosis and imbalance of osteogenic/lipogenic differentiation of MSCs. ( A ) Immunohistochemistry staining and quantitative analysis of p-p65 of rat femoral head. ( B ) NOX2, NOX4, CASP3, OSX and PPARγ mRNA of MSCs treated with DEX and BAY 11-7082. ( C ) CASP3, OSX and PPARγ protein levels of MSCs treated with DEX and BAY 11-7082. ( D ) ROS staining and quantitative analysis of MSCs treated with DEX and BAY 11-7082. ( E ) Tunel staining and flow cytometry of MSCs treated with DEX and BAY 11-7082. ( F ) ALP and Oil red O staining of MSCs treated with DEX and BAY 11-7082 for 7 or 14 days. PC, positive control, NS, no significance, * P < 0.05, ** P < 0.01, *** P < 0.001 (vs. GONFH or DEX).

Techniques Used: Immunohistochemistry, Staining, TUNEL Assay, Flow Cytometry, Positive Control


Structured Review

Proteintech anti‑ nox2
Anti‑ Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti‑ nox2/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti‑ nox2 - by Bioz Stars, 2023-10
86/100 stars

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Structured Review

Proteintech nox2 19013
Protein expression in different doses of S protein induced HUVECs. A . ACE2 and TMPRSS2 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. B . Relative expression of gray values for ACE2. C . Relative expression of gray values for TMPRSS2. D . NOX1 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. E . Relative expression of gray values for NOX1. F . <t>NOX2</t> protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. G . Relative expression of gray values for NOX2. H . NOX3 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. I . Relative expression of gray values for NOX3. J . NOX4 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. K . Relative expression of gray values for NOX4
Nox2 19013, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Synergistic effect of elevated glucose levels with SARS-CoV-2 spike protein induced NOX-dependent ROS production in endothelial cells"

Article Title: Synergistic effect of elevated glucose levels with SARS-CoV-2 spike protein induced NOX-dependent ROS production in endothelial cells

Journal: Molecular Biology Reports

doi: 10.1007/s11033-023-08504-3

Protein expression in different doses of S protein induced HUVECs. A . ACE2 and TMPRSS2 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. B . Relative expression of gray values for ACE2. C . Relative expression of gray values for TMPRSS2. D . NOX1 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. E . Relative expression of gray values for NOX1. F . NOX2 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. G . Relative expression of gray values for NOX2. H . NOX3 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. I . Relative expression of gray values for NOX3. J . NOX4 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. K . Relative expression of gray values for NOX4
Figure Legend Snippet: Protein expression in different doses of S protein induced HUVECs. A . ACE2 and TMPRSS2 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. B . Relative expression of gray values for ACE2. C . Relative expression of gray values for TMPRSS2. D . NOX1 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. E . Relative expression of gray values for NOX1. F . NOX2 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. G . Relative expression of gray values for NOX2. H . NOX3 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. I . Relative expression of gray values for NOX3. J . NOX4 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. K . Relative expression of gray values for NOX4

Techniques Used: Expressing, Western Blot


Structured Review

Proteintech nox 2
Nox 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
nox 2 - by Bioz Stars, 2023-10
86/100 stars

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Proteintech nox2
The expression of pSTAT3 Iba-1, and <t>NOX2</t> detected by immunofluorescence and western blotting. (A) Immunofluorescence co-staining of pSTAT3, Iba-1 and DAPI in 3 groups on 1 d after SAH, and each bar column represents the average number of co-stained cells under the 400X lens from the slices selected in the same section of brains in this group. (B) pSTAT3 and STAT3 expression detected by western blotting and the bar chart of corresponding relative band density. (C) NOX2 expression detected by western blotting and the bar chart of corresponding relative band density. *P < 0.05, **P < 0.01, ***P < 0.001, compared to the Sham group. # P < 0.05, ## P < 0.01, ### P < 0.001, compared to the SAH group.
Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nox2/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
nox2 - by Bioz Stars, 2023-10
86/100 stars

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1) Product Images from "Electroacupuncture alleviates early brain injury via modulating microglia polarization and suppressing neuroinflammation in a rat model of subarachnoid hemorrhage"

Article Title: Electroacupuncture alleviates early brain injury via modulating microglia polarization and suppressing neuroinflammation in a rat model of subarachnoid hemorrhage

Journal: Heliyon

doi: 10.1016/j.heliyon.2023.e14475

The expression of pSTAT3 Iba-1, and NOX2 detected by immunofluorescence and western blotting. (A) Immunofluorescence co-staining of pSTAT3, Iba-1 and DAPI in 3 groups on 1 d after SAH, and each bar column represents the average number of co-stained cells under the 400X lens from the slices selected in the same section of brains in this group. (B) pSTAT3 and STAT3 expression detected by western blotting and the bar chart of corresponding relative band density. (C) NOX2 expression detected by western blotting and the bar chart of corresponding relative band density. *P < 0.05, **P < 0.01, ***P < 0.001, compared to the Sham group. # P < 0.05, ## P < 0.01, ### P < 0.001, compared to the SAH group.
Figure Legend Snippet: The expression of pSTAT3 Iba-1, and NOX2 detected by immunofluorescence and western blotting. (A) Immunofluorescence co-staining of pSTAT3, Iba-1 and DAPI in 3 groups on 1 d after SAH, and each bar column represents the average number of co-stained cells under the 400X lens from the slices selected in the same section of brains in this group. (B) pSTAT3 and STAT3 expression detected by western blotting and the bar chart of corresponding relative band density. (C) NOX2 expression detected by western blotting and the bar chart of corresponding relative band density. *P < 0.05, **P < 0.01, ***P < 0.001, compared to the Sham group. # P < 0.05, ## P < 0.01, ### P < 0.001, compared to the SAH group.

Techniques Used: Expressing, Immunofluorescence, Western Blot, Staining


Structured Review

Proteintech nox2
The endogenous protein expression levels of ADM, calcitonin receptor-like receptor (CRLR), receptor activity-modifying protein 2 (RAMP2) and RAMP3 ( A – D ) and the effects of ADM on AT1R expression ( E ), inflammation ( F – H ), oxidative stress ( I – L ) and calcification ( M – P ) in the tunica media of aorta after 32 weeks of high-fat diet (HFD) feeding. The protein expression levels of proinflammatory cytokines TNF-α ( F ), IL-1β (G) and IL6 ( H ) were measured to reflect inflammation; the two important catalytic subunits of NADPH <t>NOX2</t> and NOX4 protein expression levels ( I , J ), ROS level ( K ) and NADPH oxidase activity ( L ) were detected to reflect oxidative stress; and the smooth-muscle lineage marker α-actin and runt-related transcription factor 2 (RUNX2, a DNA-binding transcription factor that regulates target genes in bone formation) protein expression levels ( M , N ), calcium content ( O ) and ALP activity ( P ) were determined to reflect calcification. NADPH, nicotinamide adenine dinucleotide phosphate. GAPDH was used as an internal control for Western blotting analysis. n = 4–8 rats. The values are presented as the mean ± SEM. * p < 0.05 vs. control group (two-tailed unpaired t -test, ( A – D )). Values with the same superscript letter are not significantly different and the different letters indicate significant differences between the groups ( p < 0.05, one-way ANOVA followed by Bonferroni post hoc analysis, ( E – P )).
Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nox2/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
nox2 - by Bioz Stars, 2023-10
86/100 stars

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1) Product Images from "Adrenomedullin Improves Hypertension and Vascular Remodeling partly through the Receptor-Mediated AMPK Pathway in Rats with Obesity-Related Hypertension"

Article Title: Adrenomedullin Improves Hypertension and Vascular Remodeling partly through the Receptor-Mediated AMPK Pathway in Rats with Obesity-Related Hypertension

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms24043943

The endogenous protein expression levels of ADM, calcitonin receptor-like receptor (CRLR), receptor activity-modifying protein 2 (RAMP2) and RAMP3 ( A – D ) and the effects of ADM on AT1R expression ( E ), inflammation ( F – H ), oxidative stress ( I – L ) and calcification ( M – P ) in the tunica media of aorta after 32 weeks of high-fat diet (HFD) feeding. The protein expression levels of proinflammatory cytokines TNF-α ( F ), IL-1β (G) and IL6 ( H ) were measured to reflect inflammation; the two important catalytic subunits of NADPH NOX2 and NOX4 protein expression levels ( I , J ), ROS level ( K ) and NADPH oxidase activity ( L ) were detected to reflect oxidative stress; and the smooth-muscle lineage marker α-actin and runt-related transcription factor 2 (RUNX2, a DNA-binding transcription factor that regulates target genes in bone formation) protein expression levels ( M , N ), calcium content ( O ) and ALP activity ( P ) were determined to reflect calcification. NADPH, nicotinamide adenine dinucleotide phosphate. GAPDH was used as an internal control for Western blotting analysis. n = 4–8 rats. The values are presented as the mean ± SEM. * p < 0.05 vs. control group (two-tailed unpaired t -test, ( A – D )). Values with the same superscript letter are not significantly different and the different letters indicate significant differences between the groups ( p < 0.05, one-way ANOVA followed by Bonferroni post hoc analysis, ( E – P )).
Figure Legend Snippet: The endogenous protein expression levels of ADM, calcitonin receptor-like receptor (CRLR), receptor activity-modifying protein 2 (RAMP2) and RAMP3 ( A – D ) and the effects of ADM on AT1R expression ( E ), inflammation ( F – H ), oxidative stress ( I – L ) and calcification ( M – P ) in the tunica media of aorta after 32 weeks of high-fat diet (HFD) feeding. The protein expression levels of proinflammatory cytokines TNF-α ( F ), IL-1β (G) and IL6 ( H ) were measured to reflect inflammation; the two important catalytic subunits of NADPH NOX2 and NOX4 protein expression levels ( I , J ), ROS level ( K ) and NADPH oxidase activity ( L ) were detected to reflect oxidative stress; and the smooth-muscle lineage marker α-actin and runt-related transcription factor 2 (RUNX2, a DNA-binding transcription factor that regulates target genes in bone formation) protein expression levels ( M , N ), calcium content ( O ) and ALP activity ( P ) were determined to reflect calcification. NADPH, nicotinamide adenine dinucleotide phosphate. GAPDH was used as an internal control for Western blotting analysis. n = 4–8 rats. The values are presented as the mean ± SEM. * p < 0.05 vs. control group (two-tailed unpaired t -test, ( A – D )). Values with the same superscript letter are not significantly different and the different letters indicate significant differences between the groups ( p < 0.05, one-way ANOVA followed by Bonferroni post hoc analysis, ( E – P )).

Techniques Used: Expressing, Activity Assay, Marker, Binding Assay, Western Blot, Two Tailed Test

The endogenous protein expression levels of ADM, CRLR, RAMP2 and RAMP3 in A7r5 cells ( A – D ) exposed to 200 μM PA for 24 h. The effects of ADM (10 nM) on cell viability ( E ), inflammation ( F – H ) and oxidative stress ( I – L ) stimulated by PA. The protein expression levels of proinflammatory cytokines TNF-α ( F ), IL-1β ( G ) and IL6 ( H ) were measured to reflect inflammation, and the NOX2 and NOX4 protein expression levels ( I , J ), ROS level ( K ) and NADPH activity ( L ) were detected to reflect oxidative stress. The A7r5 cells were pretreated with 10 nM ADM for 30 min subsequently treated by PA for 24 h. n = 3–6. Each value indicates mean ± SEM. * p < 0.05 vs. control group (two-tailed unpaired t -test, ( A – D )). Values with the same superscript letter are not significantly different, and the different letters indicate significant differences between the groups ( p < 0.05, one-way ANOVA followed by Bonferroni post hoc analysis, ( E – L )).
Figure Legend Snippet: The endogenous protein expression levels of ADM, CRLR, RAMP2 and RAMP3 in A7r5 cells ( A – D ) exposed to 200 μM PA for 24 h. The effects of ADM (10 nM) on cell viability ( E ), inflammation ( F – H ) and oxidative stress ( I – L ) stimulated by PA. The protein expression levels of proinflammatory cytokines TNF-α ( F ), IL-1β ( G ) and IL6 ( H ) were measured to reflect inflammation, and the NOX2 and NOX4 protein expression levels ( I , J ), ROS level ( K ) and NADPH activity ( L ) were detected to reflect oxidative stress. The A7r5 cells were pretreated with 10 nM ADM for 30 min subsequently treated by PA for 24 h. n = 3–6. Each value indicates mean ± SEM. * p < 0.05 vs. control group (two-tailed unpaired t -test, ( A – D )). Values with the same superscript letter are not significantly different, and the different letters indicate significant differences between the groups ( p < 0.05, one-way ANOVA followed by Bonferroni post hoc analysis, ( E – L )).

Techniques Used: Expressing, Activity Assay, Two Tailed Test

The effects of ADM receptor antagonist ADM22-52 (1 μM) or AMPK activation inhibitor compound C (10 μM) on ADM’s response to inflammation and oxidative stress induced by PA (200 μM) in A7r5 cells. The protein expression levels of proinflammatory cytokines TNF-α ( A ), IL-1β ( B ) and IL6 ( C ) were measured to reflect inflammation state and the NOX2 and NOX4 protein expression levels ( D , E ), ROS level ( F ), NADPH oxidase activity ( G ) and DHE fluorescence staining (intracellular ROS evaluation, H ) were detected to reflect oxidative stress state. ADM (10 nM) was administrated in A7r5 cells for 30 min, then cells were exposed to PA for further 24 h. ADM22-52 or compound C was added 30 min before ADM application, respectively. Each value indicates mean ± SEM. n = 3 to 6. Values with the same superscript letter are not significantly different and the different letters indicate significant differences between the groups ( p < 0.05, one-way ANOVA followed by Bonferroni post hoc analysis, ( A – G )).
Figure Legend Snippet: The effects of ADM receptor antagonist ADM22-52 (1 μM) or AMPK activation inhibitor compound C (10 μM) on ADM’s response to inflammation and oxidative stress induced by PA (200 μM) in A7r5 cells. The protein expression levels of proinflammatory cytokines TNF-α ( A ), IL-1β ( B ) and IL6 ( C ) were measured to reflect inflammation state and the NOX2 and NOX4 protein expression levels ( D , E ), ROS level ( F ), NADPH oxidase activity ( G ) and DHE fluorescence staining (intracellular ROS evaluation, H ) were detected to reflect oxidative stress state. ADM (10 nM) was administrated in A7r5 cells for 30 min, then cells were exposed to PA for further 24 h. ADM22-52 or compound C was added 30 min before ADM application, respectively. Each value indicates mean ± SEM. n = 3 to 6. Values with the same superscript letter are not significantly different and the different letters indicate significant differences between the groups ( p < 0.05, one-way ANOVA followed by Bonferroni post hoc analysis, ( A – G )).

Techniques Used: Activation Assay, Expressing, Activity Assay, Fluorescence, Staining

The effects of ADM (10 nM) on AT1R expression in A7r5 cells-treated by PA (200 μM, ( B )), and on the combination of PA plus Ang II-stimulated cell viability ( A ), inflammation ( C – E ) and oxidative stress ( F – I ) in A7r5 cells. The protein expression levels of proinflammatory cytokines TNF-α ( C ), IL-1β ( D ) and IL6 ( E ) were measured to reflect inflammation state and the NOX2 and NOX4 protein expression levels ( F , G ), ROS level ( H ) and NADPH oxidase activity (I) were detected to reflect oxidative stress state. ADM was administrated in A7r5 cells for 30 min, then cells were exposed to PA plus Ang II (10 nM) for further 24 h. Each value indicates mean ± SEM. n = 3 to 6. Values with the same superscript letter are not significantly different and the different letters indicate significant differences between the groups ( p < 0.05, one-way ANOVA followed by Bonferroni post hoc analysis).
Figure Legend Snippet: The effects of ADM (10 nM) on AT1R expression in A7r5 cells-treated by PA (200 μM, ( B )), and on the combination of PA plus Ang II-stimulated cell viability ( A ), inflammation ( C – E ) and oxidative stress ( F – I ) in A7r5 cells. The protein expression levels of proinflammatory cytokines TNF-α ( C ), IL-1β ( D ) and IL6 ( E ) were measured to reflect inflammation state and the NOX2 and NOX4 protein expression levels ( F , G ), ROS level ( H ) and NADPH oxidase activity (I) were detected to reflect oxidative stress state. ADM was administrated in A7r5 cells for 30 min, then cells were exposed to PA plus Ang II (10 nM) for further 24 h. Each value indicates mean ± SEM. n = 3 to 6. Values with the same superscript letter are not significantly different and the different letters indicate significant differences between the groups ( p < 0.05, one-way ANOVA followed by Bonferroni post hoc analysis).

Techniques Used: Expressing, Activity Assay

The roles of 1 μM ADM22-52 or 10 μM compound C in ADM’s response to the combination of PA plus Ang II-induced protein expressions of pro-inflammatory cytokines, including TNFα ( A ), IL-1β ( B ), IL-6 ( C ) and oxidative stress, including the protein expression of NOX2 ( D ) and NOX4 ( E ), ROS level ( F ), NADPH oxidase activity ( G ) and intracellular ROS evaluation by DHE fluorescence staining ( H ) in A7r5 cells. ADM (10 nM) was administrated in A7r5 cells for 30 min, then cells were exposed to PA plus Ang II (10 nM) for further 24 h. ADM22-52 or compound C was added 30 min before ADM application. Each value indicates mean ± SEM. n = 3 to 6. Values with the same superscript letter are not significantly different and the different letters indicate significant differences between the groups ( p < 0.05, one-way ANOVA followed by Bonferroni post hoc analysis, ( A – G )).
Figure Legend Snippet: The roles of 1 μM ADM22-52 or 10 μM compound C in ADM’s response to the combination of PA plus Ang II-induced protein expressions of pro-inflammatory cytokines, including TNFα ( A ), IL-1β ( B ), IL-6 ( C ) and oxidative stress, including the protein expression of NOX2 ( D ) and NOX4 ( E ), ROS level ( F ), NADPH oxidase activity ( G ) and intracellular ROS evaluation by DHE fluorescence staining ( H ) in A7r5 cells. ADM (10 nM) was administrated in A7r5 cells for 30 min, then cells were exposed to PA plus Ang II (10 nM) for further 24 h. ADM22-52 or compound C was added 30 min before ADM application. Each value indicates mean ± SEM. n = 3 to 6. Values with the same superscript letter are not significantly different and the different letters indicate significant differences between the groups ( p < 0.05, one-way ANOVA followed by Bonferroni post hoc analysis, ( A – G )).

Techniques Used: Expressing, Activity Assay, Fluorescence, Staining


Structured Review

Proteintech nox2
Increased NOX activity, NADPH level, and PPP stimulation following PUVA treatment. (a–d) At different time points after PUVA treatment, the enzyme activity of NOX (a) and G6PDH (d) and relative levels of NADPH (b) and NADP + (c) were determined in vitro using cell lysates. The activity of NOX and G6PDH was represented as relative light emission units/min/10 6 cells. All measurements are shown as the means ± SD from three independent experiments. (e, f) The mRNA and protein expression levels of <t>NOX2</t> and NOX4 following PUVA treatment. (g–l) ROS determination by DCF; (g) control fibroblasts treated with 5 mM NADPH for 2 hours; (h, i) PUVA-treated fibroblasts at 1 week posttreatment without (h) or with (i) preincubation of G6PDH inhibitor DHEA (100 μ M) for 2 hours followed by DCF staining; (j) PUVA-treated fibroblasts (4 weeks); (k, l) PUVA-treated fibroblasts (week 4) with preincubation of NADP + (0.5 mM) (k) or ribose-5-phosphat (R5P, 5 mM) for 2 hours followed by DCF staining.
Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Slowly Repaired Bulky DNA Damages Modulate Cellular Redox Environment Leading to Premature Senescence"

Article Title: Slowly Repaired Bulky DNA Damages Modulate Cellular Redox Environment Leading to Premature Senescence

Journal: Oxidative Medicine and Cellular Longevity

doi: 10.1155/2020/5367102

Increased NOX activity, NADPH level, and PPP stimulation following PUVA treatment. (a–d) At different time points after PUVA treatment, the enzyme activity of NOX (a) and G6PDH (d) and relative levels of NADPH (b) and NADP + (c) were determined in vitro using cell lysates. The activity of NOX and G6PDH was represented as relative light emission units/min/10 6 cells. All measurements are shown as the means ± SD from three independent experiments. (e, f) The mRNA and protein expression levels of NOX2 and NOX4 following PUVA treatment. (g–l) ROS determination by DCF; (g) control fibroblasts treated with 5 mM NADPH for 2 hours; (h, i) PUVA-treated fibroblasts at 1 week posttreatment without (h) or with (i) preincubation of G6PDH inhibitor DHEA (100 μ M) for 2 hours followed by DCF staining; (j) PUVA-treated fibroblasts (4 weeks); (k, l) PUVA-treated fibroblasts (week 4) with preincubation of NADP + (0.5 mM) (k) or ribose-5-phosphat (R5P, 5 mM) for 2 hours followed by DCF staining.
Figure Legend Snippet: Increased NOX activity, NADPH level, and PPP stimulation following PUVA treatment. (a–d) At different time points after PUVA treatment, the enzyme activity of NOX (a) and G6PDH (d) and relative levels of NADPH (b) and NADP + (c) were determined in vitro using cell lysates. The activity of NOX and G6PDH was represented as relative light emission units/min/10 6 cells. All measurements are shown as the means ± SD from three independent experiments. (e, f) The mRNA and protein expression levels of NOX2 and NOX4 following PUVA treatment. (g–l) ROS determination by DCF; (g) control fibroblasts treated with 5 mM NADPH for 2 hours; (h, i) PUVA-treated fibroblasts at 1 week posttreatment without (h) or with (i) preincubation of G6PDH inhibitor DHEA (100 μ M) for 2 hours followed by DCF staining; (j) PUVA-treated fibroblasts (4 weeks); (k, l) PUVA-treated fibroblasts (week 4) with preincubation of NADP + (0.5 mM) (k) or ribose-5-phosphat (R5P, 5 mM) for 2 hours followed by DCF staining.

Techniques Used: Activity Assay, In Vitro, Expressing, Staining


Structured Review

Proteintech anti nox 2
Anti Nox 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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anti nox 2 - by Bioz Stars, 2023-10
94/100 stars

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  • 86
    Proteintech nox2
    Niclosamide inhibits PCM induced ROS generation in VICs. (A) Representative fluorescence microscopy images of DCFDA and DHE in live-adherent VICs with corresponding DAPI staining. (B) Relative level of ROS in VICs determined by microplate assay and were calculated as a percentage of CM. Results are expressed as mean ± SEM (n=3). *** p <0.001 vs. CM; # p <0.05; ## p <0.01 vs. PCM. (C) Relative level of NADPH oxidase in VICs was determined by lucigenin chemiluminiscence assay and chemiluminiscence intensities were calculated as a percentage of CM. Results are expressed as mean ± SEM (n=3). ** p <0.01 vs. CM; # p <0.05; ## p <0.01; ### p <0.001 vs. PCM. (D, E) The gene expression of <t>NOX2</t> and its catalytic subunit p22 phox were determined by RT-qPCR. Results are expressed as mean ± SEM (n=4). ** p <0.01; *** p <0.001 vs. CM; # p <0.05; ## p <0.01; ### p <0.001 vs. PCM. (F, G) The protein expression of Nox2 and p22 phox were determined by western blotting and quantification analysis. Results are expressed as mean ± SEM (n=4). * p <0.05; ** p <0.01; vs. CM; # p <0.05; ## p <0.01 vs. PCM.
    Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Proteintech anti nox2
    Primer sequences for RT-qPCR.
    Anti Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti nox2/product/Proteintech
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    86
    Proteintech anti‑ nox2
    Primer sequences for RT-qPCR.
    Anti‑ Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti‑ nox2/product/Proteintech
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    86
    Proteintech nox2 19013
    Protein expression in different doses of S protein induced HUVECs. A . ACE2 and TMPRSS2 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. B . Relative expression of gray values for ACE2. C . Relative expression of gray values for TMPRSS2. D . NOX1 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. E . Relative expression of gray values for NOX1. F . <t>NOX2</t> protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. G . Relative expression of gray values for NOX2. H . NOX3 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. I . Relative expression of gray values for NOX3. J . NOX4 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. K . Relative expression of gray values for NOX4
    Nox2 19013, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech nox 2
    Protein expression in different doses of S protein induced HUVECs. A . ACE2 and TMPRSS2 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. B . Relative expression of gray values for ACE2. C . Relative expression of gray values for TMPRSS2. D . NOX1 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. E . Relative expression of gray values for NOX1. F . <t>NOX2</t> protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. G . Relative expression of gray values for NOX2. H . NOX3 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. I . Relative expression of gray values for NOX3. J . NOX4 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. K . Relative expression of gray values for NOX4
    Nox 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech anti nox 2
    Protein expression in different doses of S protein induced HUVECs. A . ACE2 and TMPRSS2 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. B . Relative expression of gray values for ACE2. C . Relative expression of gray values for TMPRSS2. D . NOX1 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. E . Relative expression of gray values for NOX1. F . <t>NOX2</t> protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. G . Relative expression of gray values for NOX2. H . NOX3 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. I . Relative expression of gray values for NOX3. J . NOX4 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. K . Relative expression of gray values for NOX4
    Anti Nox 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti nox 2/product/Proteintech
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    Niclosamide inhibits PCM induced ROS generation in VICs. (A) Representative fluorescence microscopy images of DCFDA and DHE in live-adherent VICs with corresponding DAPI staining. (B) Relative level of ROS in VICs determined by microplate assay and were calculated as a percentage of CM. Results are expressed as mean ± SEM (n=3). *** p <0.001 vs. CM; # p <0.05; ## p <0.01 vs. PCM. (C) Relative level of NADPH oxidase in VICs was determined by lucigenin chemiluminiscence assay and chemiluminiscence intensities were calculated as a percentage of CM. Results are expressed as mean ± SEM (n=3). ** p <0.01 vs. CM; # p <0.05; ## p <0.01; ### p <0.001 vs. PCM. (D, E) The gene expression of NOX2 and its catalytic subunit p22 phox were determined by RT-qPCR. Results are expressed as mean ± SEM (n=4). ** p <0.01; *** p <0.001 vs. CM; # p <0.05; ## p <0.01; ### p <0.001 vs. PCM. (F, G) The protein expression of Nox2 and p22 phox were determined by western blotting and quantification analysis. Results are expressed as mean ± SEM (n=4). * p <0.05; ** p <0.01; vs. CM; # p <0.05; ## p <0.01 vs. PCM.

    Journal: Biomolecules & Therapeutics

    Article Title: Niclosamide Inhibits Aortic Valve Interstitial Cell Calcification by Interfering with the GSK-3β/β-Catenin Signaling Pathway

    doi: 10.4062/biomolther.2022.146

    Figure Lengend Snippet: Niclosamide inhibits PCM induced ROS generation in VICs. (A) Representative fluorescence microscopy images of DCFDA and DHE in live-adherent VICs with corresponding DAPI staining. (B) Relative level of ROS in VICs determined by microplate assay and were calculated as a percentage of CM. Results are expressed as mean ± SEM (n=3). *** p <0.001 vs. CM; # p <0.05; ## p <0.01 vs. PCM. (C) Relative level of NADPH oxidase in VICs was determined by lucigenin chemiluminiscence assay and chemiluminiscence intensities were calculated as a percentage of CM. Results are expressed as mean ± SEM (n=3). ** p <0.01 vs. CM; # p <0.05; ## p <0.01; ### p <0.001 vs. PCM. (D, E) The gene expression of NOX2 and its catalytic subunit p22 phox were determined by RT-qPCR. Results are expressed as mean ± SEM (n=4). ** p <0.01; *** p <0.001 vs. CM; # p <0.05; ## p <0.01; ### p <0.001 vs. PCM. (F, G) The protein expression of Nox2 and p22 phox were determined by western blotting and quantification analysis. Results are expressed as mean ± SEM (n=4). * p <0.05; ** p <0.01; vs. CM; # p <0.05; ## p <0.01 vs. PCM.

    Article Snippet: The following are the primary antibodies used in this study: Runx2 (1:2,000, LsBio, Seattle, WA, USA), OPN (1:2,000, Abcam, Cambridge, UK), Nox2 (1:2,000, Proteintech, Rosemont, IL, USA), p22 phox (1:1,000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), β-catenin (1:2,000, Cell Signaling Technology, Beverly, MA, USA), p-AKT (1:2,000, Cell Signaling Technology), t-AKT (1:2,000, Cell Signaling Technology), p-ERK (1:2,000, Cell Signaling Technology), t-ERK (1:2,000, Cell Signaling Technology) and secondary antibodies [horseradish peroxidase (HRP) - conjugated anti-mouse or anti-rabbit immunoglobulin G (1:20,000; Cell Signaling Technology)].

    Techniques: Fluorescence, Microscopy, Staining, Expressing, Quantitative RT-PCR, Western Blot

    Primer sequences for RT-qPCR.

    Journal: Apoptosis

    Article Title: Glucocorticoid-induced activation of NOX/ROS/NF-κB signaling in MSCs contributes to the development of GONFH

    doi: 10.1007/s10495-023-01860-2

    Figure Lengend Snippet: Primer sequences for RT-qPCR.

    Article Snippet: Subsequently, sections were incubated with anti-RUNX2 (dilution 1:200, AB236639; Abcam, Cambridge, UK), anti-OSX (dilution 1:200, ER1914-47; Huabio, Hangzhou, Zhejiang, China), anti-PPARγ (dilution 1:300, AB19481; Abcam, Cambridge, UK), anti-fatty acid binding protein-4 (FABP4, dilution 1:300, AB92501; Abcam, Cambridge, UK), anti-NOX2 (dilution 1:500, 19013-1-AP; Proteintech, Chicago, USA), anti-NOX4 (dilution 1.

    Techniques: Sequencing

    GCs-induced exacerbation of the OS microenvironment in the necrotic region of GONFH, with enhanced NOX protein expressions and ROS levels. ( A ) ROS staining and quantitative analysis of human femoral head. ( B ) ROS staining and quantitative analysis of rat femoral head. ( C ) ROS staining and quantitative analysis of MSCs treated with 1µM DEX. ( D ) Immunohistochemistry staining and quantitative analysis of NOX2 and NOX4 of human femoral head. ( E ) Immunohistochemistry staining and quantitative analysis of NOX2 and NOX4 of rat femoral head. ( F ) NOX2 and NOX4 mRNA of MSCs treated with 1µM DEX. ( G ) NOX2 and NOX4 protein levels of MSCs treated with 1µM DEX. PC, positive control, *** P < 0.001 (vs. GONFH or DEX).

    Journal: Apoptosis

    Article Title: Glucocorticoid-induced activation of NOX/ROS/NF-κB signaling in MSCs contributes to the development of GONFH

    doi: 10.1007/s10495-023-01860-2

    Figure Lengend Snippet: GCs-induced exacerbation of the OS microenvironment in the necrotic region of GONFH, with enhanced NOX protein expressions and ROS levels. ( A ) ROS staining and quantitative analysis of human femoral head. ( B ) ROS staining and quantitative analysis of rat femoral head. ( C ) ROS staining and quantitative analysis of MSCs treated with 1µM DEX. ( D ) Immunohistochemistry staining and quantitative analysis of NOX2 and NOX4 of human femoral head. ( E ) Immunohistochemistry staining and quantitative analysis of NOX2 and NOX4 of rat femoral head. ( F ) NOX2 and NOX4 mRNA of MSCs treated with 1µM DEX. ( G ) NOX2 and NOX4 protein levels of MSCs treated with 1µM DEX. PC, positive control, *** P < 0.001 (vs. GONFH or DEX).

    Article Snippet: Subsequently, sections were incubated with anti-RUNX2 (dilution 1:200, AB236639; Abcam, Cambridge, UK), anti-OSX (dilution 1:200, ER1914-47; Huabio, Hangzhou, Zhejiang, China), anti-PPARγ (dilution 1:300, AB19481; Abcam, Cambridge, UK), anti-fatty acid binding protein-4 (FABP4, dilution 1:300, AB92501; Abcam, Cambridge, UK), anti-NOX2 (dilution 1:500, 19013-1-AP; Proteintech, Chicago, USA), anti-NOX4 (dilution 1.

    Techniques: Staining, Immunohistochemistry, Positive Control

    DPI counteracted the OS-promoting effects of DEX and reversed apoptosis and imbalance in osteogenic/lipogenic differentiation of MSCs. ( A ) ROS staining and quantitative analysis of MSCs treated with DEX and DPI. ( B ) NOX2, NOX4, CASP3, OSX and PPARγ mRNA of MSCs treated with DEX and DPI. ( C ) NOX2, NOX4, CASP3, OSX and PPARγ protein levels of MSCs treated with DEX and DPI. ( D ) Tunel staining and flow cytometry of MSCs treated with DEX and DPI. ( E ) ALP and Oil red O staining of MSCs treated with DEX and DPI for 7 or 14 days. PC, positive control, * P < 0.05, ** P < 0.01, *** P < 0.001 (vs. DEX).

    Journal: Apoptosis

    Article Title: Glucocorticoid-induced activation of NOX/ROS/NF-κB signaling in MSCs contributes to the development of GONFH

    doi: 10.1007/s10495-023-01860-2

    Figure Lengend Snippet: DPI counteracted the OS-promoting effects of DEX and reversed apoptosis and imbalance in osteogenic/lipogenic differentiation of MSCs. ( A ) ROS staining and quantitative analysis of MSCs treated with DEX and DPI. ( B ) NOX2, NOX4, CASP3, OSX and PPARγ mRNA of MSCs treated with DEX and DPI. ( C ) NOX2, NOX4, CASP3, OSX and PPARγ protein levels of MSCs treated with DEX and DPI. ( D ) Tunel staining and flow cytometry of MSCs treated with DEX and DPI. ( E ) ALP and Oil red O staining of MSCs treated with DEX and DPI for 7 or 14 days. PC, positive control, * P < 0.05, ** P < 0.01, *** P < 0.001 (vs. DEX).

    Article Snippet: Subsequently, sections were incubated with anti-RUNX2 (dilution 1:200, AB236639; Abcam, Cambridge, UK), anti-OSX (dilution 1:200, ER1914-47; Huabio, Hangzhou, Zhejiang, China), anti-PPARγ (dilution 1:300, AB19481; Abcam, Cambridge, UK), anti-fatty acid binding protein-4 (FABP4, dilution 1:300, AB92501; Abcam, Cambridge, UK), anti-NOX2 (dilution 1:500, 19013-1-AP; Proteintech, Chicago, USA), anti-NOX4 (dilution 1.

    Techniques: Staining, TUNEL Assay, Flow Cytometry, Positive Control

    BAY partially reversed DEX-induced apoptosis and imbalance of osteogenic/lipogenic differentiation of MSCs. ( A ) Immunohistochemistry staining and quantitative analysis of p-p65 of rat femoral head. ( B ) NOX2, NOX4, CASP3, OSX and PPARγ mRNA of MSCs treated with DEX and BAY 11-7082. ( C ) CASP3, OSX and PPARγ protein levels of MSCs treated with DEX and BAY 11-7082. ( D ) ROS staining and quantitative analysis of MSCs treated with DEX and BAY 11-7082. ( E ) Tunel staining and flow cytometry of MSCs treated with DEX and BAY 11-7082. ( F ) ALP and Oil red O staining of MSCs treated with DEX and BAY 11-7082 for 7 or 14 days. PC, positive control, NS, no significance, * P < 0.05, ** P < 0.01, *** P < 0.001 (vs. GONFH or DEX).

    Journal: Apoptosis

    Article Title: Glucocorticoid-induced activation of NOX/ROS/NF-κB signaling in MSCs contributes to the development of GONFH

    doi: 10.1007/s10495-023-01860-2

    Figure Lengend Snippet: BAY partially reversed DEX-induced apoptosis and imbalance of osteogenic/lipogenic differentiation of MSCs. ( A ) Immunohistochemistry staining and quantitative analysis of p-p65 of rat femoral head. ( B ) NOX2, NOX4, CASP3, OSX and PPARγ mRNA of MSCs treated with DEX and BAY 11-7082. ( C ) CASP3, OSX and PPARγ protein levels of MSCs treated with DEX and BAY 11-7082. ( D ) ROS staining and quantitative analysis of MSCs treated with DEX and BAY 11-7082. ( E ) Tunel staining and flow cytometry of MSCs treated with DEX and BAY 11-7082. ( F ) ALP and Oil red O staining of MSCs treated with DEX and BAY 11-7082 for 7 or 14 days. PC, positive control, NS, no significance, * P < 0.05, ** P < 0.01, *** P < 0.001 (vs. GONFH or DEX).

    Article Snippet: Subsequently, sections were incubated with anti-RUNX2 (dilution 1:200, AB236639; Abcam, Cambridge, UK), anti-OSX (dilution 1:200, ER1914-47; Huabio, Hangzhou, Zhejiang, China), anti-PPARγ (dilution 1:300, AB19481; Abcam, Cambridge, UK), anti-fatty acid binding protein-4 (FABP4, dilution 1:300, AB92501; Abcam, Cambridge, UK), anti-NOX2 (dilution 1:500, 19013-1-AP; Proteintech, Chicago, USA), anti-NOX4 (dilution 1.

    Techniques: Immunohistochemistry, Staining, TUNEL Assay, Flow Cytometry, Positive Control

    Protein expression in different doses of S protein induced HUVECs. A . ACE2 and TMPRSS2 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. B . Relative expression of gray values for ACE2. C . Relative expression of gray values for TMPRSS2. D . NOX1 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. E . Relative expression of gray values for NOX1. F . NOX2 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. G . Relative expression of gray values for NOX2. H . NOX3 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. I . Relative expression of gray values for NOX3. J . NOX4 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. K . Relative expression of gray values for NOX4

    Journal: Molecular Biology Reports

    Article Title: Synergistic effect of elevated glucose levels with SARS-CoV-2 spike protein induced NOX-dependent ROS production in endothelial cells

    doi: 10.1007/s11033-023-08504-3

    Figure Lengend Snippet: Protein expression in different doses of S protein induced HUVECs. A . ACE2 and TMPRSS2 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. B . Relative expression of gray values for ACE2. C . Relative expression of gray values for TMPRSS2. D . NOX1 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. E . Relative expression of gray values for NOX1. F . NOX2 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. G . Relative expression of gray values for NOX2. H . NOX3 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. I . Relative expression of gray values for NOX3. J . NOX4 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. K . Relative expression of gray values for NOX4

    Article Snippet: The antibodies to ACE2 (#21115), TMPRSS2(#14437), NOX2(#19013), NOX4(#14347), and GAPDH (#60004) were obtained from Proteintech (Rosemont, IL).

    Techniques: Expressing, Western Blot