Journal: International Journal of Molecular Sciences

Article Title: A Novel NOX Inhibitor Treatment Attenuates Parkinson’s Disease-Related Pathology in Mouse Models

doi: 10.3390/ijms23084262

Figure Lengend Snippet: The compound-6 effectively inhibits NOX-1, -2 and -4, based on IC 50 analysis.

Article Snippet: The primary antibodies pertaining to each protein analyzed were diluted at 1:1000 (p-Ser-129-α-synuclein, Cell signaling, cat# 23706S; NOX-1,2 rabbit antibody, Proteintech 17772–1-AP and 19013–1-AP) and incubated at 4 °C overnight.

Techniques:

Journal: BMC Cardiovascular Disorders

Article Title: Role of peroxisome proliferators-activated receptor-gamma in advanced glycation end product-mediated functional loss of voltage-gated potassium channel in rat coronary arteries

doi: 10.1186/s12872-020-01613-y

Figure Lengend Snippet: Protein expressions in incubated RSCAs detected by Western blot. The RSCAs were incubated with DMEM, 200 μg/mL BSA, 200 μg/mL AGE, 200 μg/mL AGE+ 1 mM ALA, 200 μg/mL AGE+ 0.1 mM PIO, or 200 μg/mL AGE + 0.1 mM PIO + 0.1 mM GW9662 for 24 h. a Representative blots for RAGE, Kv1.2, Kv1.5, PPAR-γ and NOX-2. β-actin was used as an internal reference. b-d Mean data for protein expression levels. n = 3 per group. * P < 0.05 vs. BSA; # P < 0.05 vs. AGE; § P < 0.05 vs. AGE + PIO

Article Snippet: Equal amounts of protein were separated by 10% SDS-PAGE, transferred to PVDF membranes, and stained with the following primary antibodies (all from Abcam, Cambridge, UK): polyclonal rabbit anti-RAGE (1:500), monoclonal mouse anti-Kv1.2 (1:1000), monoclonal mouse anti-Kv1.5 (1:1000), monoclonal rabbit anti-PPAR-γ (1:1000), monoclonal rabbit anti-NOX-2 (1:1000), and anti-β-actin (1:10000), followed by incubation with HRP-conjugated goat anti-mouse IgG antibody (1:20000, Proteintech Group Inc., IL, USA).

Techniques: Incubation, Western Blot, Expressing

Journal: BMC Cardiovascular Disorders

Article Title: Role of peroxisome proliferators-activated receptor-gamma in advanced glycation end product-mediated functional loss of voltage-gated potassium channel in rat coronary arteries

doi: 10.1186/s12872-020-01613-y

Figure Lengend Snippet: Protein expressions in cultured CSMCs detected by Western blot. The CSMCs were incubated with DMEM, 100 μg/mL BSA, 100 μg/mL AGE, 100 μg/mL AGE+ 10 μM ALA, 100 μg/mL AGE+ 1 μM PIO, or 100 μg/mL AGE+ 1 μM PIO + 1 μM GW9662 for 24 h. a Representative blots for RAGE, Kv1.2, Kv1.5, PPAR-γ and NOX-2. β-actin was used as an internal reference. b-d Mean data for protein expression levels. n = 3 per group. * P < 0.05 vs. BSA; # P < 0.05 vs. AGE; § P < 0.05 vs. AGE + PIO

Article Snippet: Equal amounts of protein were separated by 10% SDS-PAGE, transferred to PVDF membranes, and stained with the following primary antibodies (all from Abcam, Cambridge, UK): polyclonal rabbit anti-RAGE (1:500), monoclonal mouse anti-Kv1.2 (1:1000), monoclonal mouse anti-Kv1.5 (1:1000), monoclonal rabbit anti-PPAR-γ (1:1000), monoclonal rabbit anti-NOX-2 (1:1000), and anti-β-actin (1:10000), followed by incubation with HRP-conjugated goat anti-mouse IgG antibody (1:20000, Proteintech Group Inc., IL, USA).

Techniques: Cell Culture, Western Blot, Incubation, Expressing