anti nicotinamide adenine dinucleotide 3 phosphate nadph oxidase subunit p22 phox  (Santa Cruz Biotechnology)

Journal: Toxics

Article Title: Hypoxia-Induced Kidney Injury in Newborn Rats

doi: 10.3390/toxics11030260

Figure Lengend Snippet: Hypoxic kidney injury involves oxidative stress, apoptosis, and inflammatory signaling. ( A ) Western blot analysis shows the oxidative-stress-related proteins, including P22, P47, NOX2, and NOX4. ( B ) Western blot analysis shows apoptosis-related proteins, including p-P38, Casp-9, and cleaved casp-3. ( C ) Western blot analysis shows the inflammatory-related proteins, including TNF-α and NF-κB. Quantitative analysis was performed for each blot. The values are presented as mean ± standard deviation. * p < 0.05, ** p < 0.01, versus the normoxic group. The p -values were estimated via Mann–Whitney U test ( n = 6). N, normoxia; H, hypoxia. P22, nicotinamide adenine dinucleotide 3-phosphate (NADPH) oxidase subunit p22-phox; P47, NADPH oxidase subunit p47-phox; NOX2, NADPH oxidase 2; NOX4, NADPH oxidase 4; p-P38, phospho-p38 mitogen-activated protein kinase (Thr180/Tyr182); P38, p38 MAP kinase; Casp-9, caspase-9; cleaved Casp 3, cleaved-caspase-3; TNF-α, tumor necrosis factor-α; NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells.

Article Snippet: The membrane was blocked in PBST buffer with 5% nonfat milk and 5% bovine serum albumin before being incubated with anti-hypoxia-inducible factors, alpha subunit (HIF-1α) (20960–1AP, Proteintech), anti-heme oxygenase-1 (HO-1) (ADI SPA-895, Enzo Biochem, Inc., Farmingdale, NY, USA), anti-NGAL (ab63929, Abcam), anti- nicotinamide adenine dinucleotide 3-phosphate (NADPH) oxidase subunit p22-phox (P22) (sc271968, Santa Cruz Biotechnology, Dallas, TX, USA), NADPH oxidase subunit p47-phox (P47) (sc-17845, Santa Cruz), anti- NADPH oxidase 2 phox (NOX2) (ab129068, Abcam), anti- NADPH oxidase 4 phox (NOX4) (14347–1-Ig, Proteintech), anti-collagen type I (67288–1-Ig, Proteintech), anti-fibroblast growth factor 23 (FGF23) (Ls-C411984, Lifespan Biosciences, Seattle, WA, USA), phospho-p38 mitogen-activated protein kinase (MAPK) (Thr180/Tyr182) (p-P38) (#9211, Cell signaling, Danvers, MA, USA), p38 MAPK (P38) (9212S, Cell Signaling), anti-caspase-9 (GTX112888, GeneTex, Irvine, CA, USA), anti-cleaved caspase-3 (#9664, Cell Signaling), anti-tumor necrosis factor-α (TNF-α) (PA1079, Boster biological technology, Pleasanton, CA, USA), anti-nuclear factor kappa B (NF-κB) (10745–1-AP, Proteintech), anti-actin (MAB1501, Merck KGaA, Darmstadt, Germany), anti-alpha tubulin (ab7291 Abcam), and anti-GAPDH (60004–1-Ig, Proteintech) antibodies.

Techniques: Western Blot, Standard Deviation, MANN-WHITNEY