antibodies against gapdh  (Cell Signaling Technology Inc)

 
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  • 99
    Name:
    Phospho Histone H3 Ser10 Blocking Peptide
    Description:
    This peptide is used to block Phospho Histone H3 Ser10 D2C8 XP Rabbit mAb 3377 reactivity
    Catalog Number:
    1000
    Price:
    None
    Category:
    Experimental Controls
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    Structured Review

    Cell Signaling Technology Inc antibodies against gapdh
    This peptide is used to block Phospho Histone H3 Ser10 D2C8 XP Rabbit mAb 3377 reactivity
    https://www.bioz.com/result/antibodies against gapdh/product/Cell Signaling Technology Inc
    Average 99 stars, based on 391 article reviews
    Price from $9.99 to $1999.99
    antibodies against gapdh - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Western Blot:

    Article Title: Constitutively active Ras negatively regulates Erk MAP kinase through induction of MAP kinase phosphatase 3 (MKP3) in NIH3T3 cells
    Article Snippet: .. Western blot analysis was performed according to standard procedures, as described previously , using an antibody against either H-Ras (1 : 500; Oncogene), phospho-Raf1 (Ser259; 1 : 1,000; Cell Signaling), phospho-Erk1/2 (Thr202/Tyr204; 1 : 1000; Cell Signaling), phospho-RSK (Thr573; 1 : 1,000; Cell Signaling), phospho-Akt (Ser473; 1 : 1,000; Cell Signaling), Erk2 (1 : 5,000; Santa Cruz Biotechnology), phospho-Elk1 (Ser383; 1 : 1,000; Cell Signaling), MKP3 (1 : 5,000; Cell Signaling), or GAPDH (1 : 2,000; Santa Cruz Biotechnology). .. Northern blot analysis Total RNA samples (10 μg) were separated by electrophoresis on a formaldehyde/agarose gel and transferred to a Hybond N+ nylon membrane (Amersham Pharmacia Biotech).

    Article Title: Antifibrotic Effects of Hepatocyte Growth Factor on Endothelial-to-Mesenchymal Transition via Transforming Growth Factor-Beta1 (TGF-β1)/Smad and Akt/mTOR/P70S6K Signaling Pathways
    Article Snippet: .. Then, we performed western blotting by incubation with primary antibodies of anti-GAPDH (1: 200, Abcam, Cambridge, MA, USA), anti-α-SMA (1: 2,500, Abcam), anti-CD31 (1: 1,000, Abcam), anti-TGF-β1 (1: 250, Abcam), anti-HGF (1: 500, Abcam), anti-Akt (1: 1,000, Cell Signaling Technology, Beverly, MA, USA), anti-phospho-Akt (Ser473, 1: 1,000, Cell Signaling Technology), anti-mTOR (1: 1,000, Cell Signaling Technology,), anti-phospho-mTOR (Ser2448, 1: 1,000, Cell Signaling Technology), anti-p70S6K (1: 1,000, Cell Signaling Technology), anti-phospho-p70S6K (Thr389, 1: 1,000, Cell Signaling Technology), anti-Erk 1/2 (1: 1,000, Cell Signaling Technology) anti-phospho-Erk 1/2 (Thr202/Tyr204, 1: 1,000, Cell Signaling Technology), anti-c-Jun (1: 1,000, Cell Signaling Technology) and anti-phospho-c-Jun (Ser73, 1: 1,000, Cell Signaling Technology), anti-Smad 2 (1: 1,000, Cell Signaling Technology), anti-phospho-Smad 2 (Ser465/467, 1: 1,000, Cell Signaling Technology), anti-Smad 3 (1: 1,000, Cell Signaling Technology) and anti-phospho-Smad 3 (Ser423/425, 1: 1,000, Cell Signaling Technology), followed by incubation with anti-rabbit or anti-mouse secondary antibody (1: 1,000, Abcam). .. Relative abundance of proteins was measured, and GAPDH expression was considered as an internal reference and quantified band under the Odyssey infrared imaging system (LI-COR Biotechnology, Nebraska).

    Incubation:

    Article Title: Neural Differentiation of Human Adipose Tissue-Derived Stem Cells Involves Activation of the Wnt5a/JNK Signalling
    Article Snippet: .. After blocking with 1% skim milk (Sigma Chemical Co.) in PBS-T, the membranes were incubated with specific antibodies for Wnt3a (Cell Signaling Technology, Inc., Danvers, MA, USA, 1 : 1,000), Wnt5a/b (Cell Signaling Technology, 1 : 4,000), Dvl2 (Cell Signaling Technology, 1 : 1,000), Naked1 (Cell Signaling Technology, 1 : 1,000), Axin 1 (Cell Signaling Technology, 1 : 2,000), phosphor-JNK (Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1 : 4,000), JNK (Santa Cruz Biotechnology, 1 : 4,000), phosphor-ERK1/2 (Santa Cruz Biotechnology, 1 : 1,000), ERK1/2 (Santa Cruz Biotechnology, 1 : 4,000), phosphor-PKC (Santa Cruz Biotechnology, 1 : 4,000), PKC (Santa Cruz Biotechnology, 1 : 1,000), phosphor-GSK3β (Cell Signaling Technology, 1 : 3,000), GSK3β (Cell Signaling Technology, 1 : 3,000), β -catenin (Cell Signaling Technology, 1 : 4,000), or GAPDH (Santa Cruz Biotechnology, 1 : 4,000). .. Following several washes, membranes were subsequently incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG antibody (Cell Signaling Technology).

    Article Title: Antifibrotic Effects of Hepatocyte Growth Factor on Endothelial-to-Mesenchymal Transition via Transforming Growth Factor-Beta1 (TGF-β1)/Smad and Akt/mTOR/P70S6K Signaling Pathways
    Article Snippet: .. Then, we performed western blotting by incubation with primary antibodies of anti-GAPDH (1: 200, Abcam, Cambridge, MA, USA), anti-α-SMA (1: 2,500, Abcam), anti-CD31 (1: 1,000, Abcam), anti-TGF-β1 (1: 250, Abcam), anti-HGF (1: 500, Abcam), anti-Akt (1: 1,000, Cell Signaling Technology, Beverly, MA, USA), anti-phospho-Akt (Ser473, 1: 1,000, Cell Signaling Technology), anti-mTOR (1: 1,000, Cell Signaling Technology,), anti-phospho-mTOR (Ser2448, 1: 1,000, Cell Signaling Technology), anti-p70S6K (1: 1,000, Cell Signaling Technology), anti-phospho-p70S6K (Thr389, 1: 1,000, Cell Signaling Technology), anti-Erk 1/2 (1: 1,000, Cell Signaling Technology) anti-phospho-Erk 1/2 (Thr202/Tyr204, 1: 1,000, Cell Signaling Technology), anti-c-Jun (1: 1,000, Cell Signaling Technology) and anti-phospho-c-Jun (Ser73, 1: 1,000, Cell Signaling Technology), anti-Smad 2 (1: 1,000, Cell Signaling Technology), anti-phospho-Smad 2 (Ser465/467, 1: 1,000, Cell Signaling Technology), anti-Smad 3 (1: 1,000, Cell Signaling Technology) and anti-phospho-Smad 3 (Ser423/425, 1: 1,000, Cell Signaling Technology), followed by incubation with anti-rabbit or anti-mouse secondary antibody (1: 1,000, Abcam). .. Relative abundance of proteins was measured, and GAPDH expression was considered as an internal reference and quantified band under the Odyssey infrared imaging system (LI-COR Biotechnology, Nebraska).

    Article Title: Effects of Astragalus Polysaccharides on Dysfunction of Mitochondrial Dynamics Induced by Oxidative Stress
    Article Snippet: .. The membranes were incubated with primary antibodies including anti-PGC-1, anti-MnSOD, anti-p53, and anti-GAPDH (1 : 1,000; Santa Cruz Biotechnology) as well as anti-Atg7, anti-p62, and anti-LC3 (1 : 1,000; Cell Signaling) at 4°C overnight. .. Then, the membranes were incubated with anti-rabbit or anti-mouse antibodies at room temperature for 1 h. The protein bands were captured and documented through a CCD system (Image Station 2000MM, Kodak, Rochester, NY, USA) or a gel image analysis system (ChemiDox XRS, Bio-Rad, USA).

    Article Title: Leukemia Inhibitory Factor Represses GnRH Gene Expression via cFOS during Inflammation in Male Mice
    Article Snippet: .. Membranes were blocked in TBST (20 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.1% Tween-20) with 10% non-fat milk for 2 h at room temperature and incubated overnight with specific antibodies for p-STAT3 (1: 1,000; 9134; Cell Signaling, Danvers, MA, USA), STAT3 (1: 1,000, 9139; Cell Signaling), p-p38 (1: 1,000, 9211; Cell Signaling), p38 (1: 1,000, 9212; Cell Signaling), p-ERK½ (1: 1,000, 9101; Cell Signaling), ERK½ (1: 1,000, 9102; Cell Signaling), p-JNK (1: 1,000, 9255; Cell Signaling), JNK (1: 1,000, 9252; Cell Signaling), OCT-1 (1: 500, ab66132, Abcam, Cambridge, UK), cFOS (1: 1,000, SC-52; Santa Cruz Biotechnology), and β-tubulin (1: 1,000, SC-9104; Santa Cruz Biotechnology). .. The bands were visualized with horseradish peroxidase (HRP)-linked secondary antibodies and enhanced chemiluminescence reagent (Amersham Bioscience), as described by the manufacturer.

    Article Title: Study on the Mechanism of mTOR-Mediated Autophagy during Electroacupuncture Pretreatment against Cerebral Ischemic Injury
    Article Snippet: .. A 60 μ g aliquot of proteins from each sample was extracted by applying 10% SDS-polyacrylamide gel electrophoresis and transferred to a nitrocellulose membrane, which was then incubated with the specific antibodies against LC3 (#4108; 1 : 1,000; Cell Signaling Technology, Inc., Danvers, MA,USA), p-mTOR (#2971, Cell Signaling Technology, Inc., Danvers, MA, USA), and mTOR (#2972, Cell Signaling Technology, Inc., Danvers, MA, USA) at 4°C overnight. .. Afterward, the membranes were incubated with a horseradish peroxidase-conjugated secondary antibody at room temperature for 1 h. An enhanced chemiluminescent autoradiography was adopted to detect immunoreactivity based on manufacturer's instructions.

    Article Title: Neuroplastic Correlates in the mPFC Underlying the Impairment of Stress-Coping Ability and Cognitive Flexibility in Adult Rats Exposed to Chronic Mild Stress during Adolescence
    Article Snippet: .. After being washed in TBST (10 min × 3), the membrane was incubated at RT for 2 h on a shaker with primary antibodies: rabbit monoclonal ERK1/2 antibodies (1 : 1,000, Cell Signaling Technology Inc., Beverly, MA, USA) and rabbit monoclonal pERK1/2 antibodies (1 : 2,000, Cell Signaling Technology Inc.). .. After further washing in TBST (10 min × 3), the membrane was incubated at RT for 1 h on a shaker with secondary antibodies, HRP-conjugated goat anti-rabbit IgG (1 : 4,000, Zhongshan Golden Bridge Biotechnology, Beijing, China), and then washed again.

    Blocking Assay:

    Article Title: Neural Differentiation of Human Adipose Tissue-Derived Stem Cells Involves Activation of the Wnt5a/JNK Signalling
    Article Snippet: .. After blocking with 1% skim milk (Sigma Chemical Co.) in PBS-T, the membranes were incubated with specific antibodies for Wnt3a (Cell Signaling Technology, Inc., Danvers, MA, USA, 1 : 1,000), Wnt5a/b (Cell Signaling Technology, 1 : 4,000), Dvl2 (Cell Signaling Technology, 1 : 1,000), Naked1 (Cell Signaling Technology, 1 : 1,000), Axin 1 (Cell Signaling Technology, 1 : 2,000), phosphor-JNK (Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1 : 4,000), JNK (Santa Cruz Biotechnology, 1 : 4,000), phosphor-ERK1/2 (Santa Cruz Biotechnology, 1 : 1,000), ERK1/2 (Santa Cruz Biotechnology, 1 : 4,000), phosphor-PKC (Santa Cruz Biotechnology, 1 : 4,000), PKC (Santa Cruz Biotechnology, 1 : 1,000), phosphor-GSK3β (Cell Signaling Technology, 1 : 3,000), GSK3β (Cell Signaling Technology, 1 : 3,000), β -catenin (Cell Signaling Technology, 1 : 4,000), or GAPDH (Santa Cruz Biotechnology, 1 : 4,000). .. Following several washes, membranes were subsequently incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG antibody (Cell Signaling Technology).

    Nucleic Acid Electrophoresis:

    Article Title: Study on the Mechanism of mTOR-Mediated Autophagy during Electroacupuncture Pretreatment against Cerebral Ischemic Injury
    Article Snippet: .. A 60 μ g aliquot of proteins from each sample was extracted by applying 10% SDS-polyacrylamide gel electrophoresis and transferred to a nitrocellulose membrane, which was then incubated with the specific antibodies against LC3 (#4108; 1 : 1,000; Cell Signaling Technology, Inc., Danvers, MA,USA), p-mTOR (#2971, Cell Signaling Technology, Inc., Danvers, MA, USA), and mTOR (#2972, Cell Signaling Technology, Inc., Danvers, MA, USA) at 4°C overnight. .. Afterward, the membranes were incubated with a horseradish peroxidase-conjugated secondary antibody at room temperature for 1 h. An enhanced chemiluminescent autoradiography was adopted to detect immunoreactivity based on manufacturer's instructions.

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  • 91
    Cell Signaling Technology Inc anti nfatc1 cat no 8032 antibody
    Anti Nfatc1 Cat No 8032 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti nfatc1 cat no 8032 antibody/product/Cell Signaling Technology Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti nfatc1 cat no 8032 antibody - by Bioz Stars, 2020-09
    91/100 stars
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