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1) Product Images from "Semaphorin 3G exacerbates joint inflammation through the accumulation and proliferation of macrophages in the synovium"
Article Title: Semaphorin 3G exacerbates joint inflammation through the accumulation and proliferation of macrophages in the synovium
Figure Legend Snippet: Nrp2 expression in activated macrophages during joint inflammation A. Nrp2 expression on immune cells in CIA synovium. The hind paws of CIA-induced mice were digested and subjected to flow cytometric analysis. The representative histograms of Nrp2 staining and the cumulative data are shown. Data are expressed means ± SD. N = 3 from 3 independent experiments. B. The characteristics of Nrp2-positive macrophages. The representative histograms of CD86 and MHC class II expression on joint-infiltrating macrophages (left) and their cumulative data (right) are shown. N = 6 from 2 independent experiments. C. The expression of Nrp2 on BMMs. BMMs were cultured under indicated conditions, and Nrp2 expression was determined by flow cytometry. The representative histograms and the cumulative data are shown. N = 3 from 3 independent experiments. D. NRP2 expression in synovium-infiltrating cells in RA. The deposited single-cell RNA-seq data was re-analyzed, and several cell types were defined by UMAP. Expression of NRP2 is indicated with purple dots. The statistical analyses were performed using an unpaired t-test.
Techniques Used: Expressing, Mouse Assay, Staining, Cell Culture, Flow Cytometry, RNA Sequencing Assay