anti muscarinic acetylcholine receptor m1 antibody  (Millipore)

 
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    Structured Review

    Millipore anti muscarinic acetylcholine receptor m1 antibody
    M1 mAChR expression and localisation in transfected HEK293 cells. (A) PCR was conducted to assess M1 expression by vector-transfected (HEK293-Vec) and M1 transfected HEK293 cells (HEK293-M1) using primers for M1 mAChR. The integrity of cDNA samples was confirmed using GAPDH. Samples are as follows; HEK293-Vec cDNA in lane 1, HEK293-Vec -RT/RNA in lane 2, HEK293-M1 cDNA in lane 3, and HEK293-M1–RT/RNA in lane 4. Samples are representative of those used in subsequent functional studies. (B) Immunocytochemical localisation of M1 receptors in HEK293-M1 cells using anti-HA.11 antibody (middle panel) and <t>M1</t> antibody (right-hand panel). Left panel shows no primary control Image. (C) Analysis of M1 receptor cell-surface expression and internalization by carbachol. Cell surface receptors were live-labeled (see methods) using the HA.11antibody prior to stimulation with water-control or carbachol treatment. The M1 mAChRs were typically localised at the plasma membrane after water treatment, but after 1 h carbachol treatment for the M1 mAChRs were internalised, as shown by the increased punctate cytoplasmic staining (arrows) and reduced staining intensity on the cell surfaces. Scale bar: 50 µm. Data are representative of at least three independent experiments. (D) shows the time-course of M1 receptor internalization after carbachol addition using the granularity assay in Metamorph to measure internalized receptors (as intracellular granules). The graph shows that 5–60 minutes after carbachol addition there is internalization of M1 receptors.
    Anti Muscarinic Acetylcholine Receptor M1 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti muscarinic acetylcholine receptor m1 antibody/product/Millipore
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    anti muscarinic acetylcholine receptor m1 antibody - by Bioz Stars, 2022-11
    93/100 stars

    Images

    1) Product Images from "M1 Muscarinic Receptor Activation Mediates Cell Death in M1-HEK293 Cells"

    Article Title: M1 Muscarinic Receptor Activation Mediates Cell Death in M1-HEK293 Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0072011

    M1 mAChR expression and localisation in transfected HEK293 cells. (A) PCR was conducted to assess M1 expression by vector-transfected (HEK293-Vec) and M1 transfected HEK293 cells (HEK293-M1) using primers for M1 mAChR. The integrity of cDNA samples was confirmed using GAPDH. Samples are as follows; HEK293-Vec cDNA in lane 1, HEK293-Vec -RT/RNA in lane 2, HEK293-M1 cDNA in lane 3, and HEK293-M1–RT/RNA in lane 4. Samples are representative of those used in subsequent functional studies. (B) Immunocytochemical localisation of M1 receptors in HEK293-M1 cells using anti-HA.11 antibody (middle panel) and M1 antibody (right-hand panel). Left panel shows no primary control Image. (C) Analysis of M1 receptor cell-surface expression and internalization by carbachol. Cell surface receptors were live-labeled (see methods) using the HA.11antibody prior to stimulation with water-control or carbachol treatment. The M1 mAChRs were typically localised at the plasma membrane after water treatment, but after 1 h carbachol treatment for the M1 mAChRs were internalised, as shown by the increased punctate cytoplasmic staining (arrows) and reduced staining intensity on the cell surfaces. Scale bar: 50 µm. Data are representative of at least three independent experiments. (D) shows the time-course of M1 receptor internalization after carbachol addition using the granularity assay in Metamorph to measure internalized receptors (as intracellular granules). The graph shows that 5–60 minutes after carbachol addition there is internalization of M1 receptors.
    Figure Legend Snippet: M1 mAChR expression and localisation in transfected HEK293 cells. (A) PCR was conducted to assess M1 expression by vector-transfected (HEK293-Vec) and M1 transfected HEK293 cells (HEK293-M1) using primers for M1 mAChR. The integrity of cDNA samples was confirmed using GAPDH. Samples are as follows; HEK293-Vec cDNA in lane 1, HEK293-Vec -RT/RNA in lane 2, HEK293-M1 cDNA in lane 3, and HEK293-M1–RT/RNA in lane 4. Samples are representative of those used in subsequent functional studies. (B) Immunocytochemical localisation of M1 receptors in HEK293-M1 cells using anti-HA.11 antibody (middle panel) and M1 antibody (right-hand panel). Left panel shows no primary control Image. (C) Analysis of M1 receptor cell-surface expression and internalization by carbachol. Cell surface receptors were live-labeled (see methods) using the HA.11antibody prior to stimulation with water-control or carbachol treatment. The M1 mAChRs were typically localised at the plasma membrane after water treatment, but after 1 h carbachol treatment for the M1 mAChRs were internalised, as shown by the increased punctate cytoplasmic staining (arrows) and reduced staining intensity on the cell surfaces. Scale bar: 50 µm. Data are representative of at least three independent experiments. (D) shows the time-course of M1 receptor internalization after carbachol addition using the granularity assay in Metamorph to measure internalized receptors (as intracellular granules). The graph shows that 5–60 minutes after carbachol addition there is internalization of M1 receptors.

    Techniques Used: Expressing, Transfection, Polymerase Chain Reaction, Plasmid Preparation, Functional Assay, Labeling, Staining

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    Millipore anti muscarinic acetylcholine receptor m1 antibody produced in rabbit
    The effect of VGLUT3 deletion on glutamate and nonglutamate receptors expression in the mouse hippocampus. A–G , Representative immunoblots and quantification of cell surface and total levels of mGluR5 ( A ), mGluR2/3 ( B ), NMDAR2A ( C ), NMDAR2B ( D ), GluA1 ( E ), D1R ( F ), and <t>M1</t> <t>mAChR</t> ( G ) in hippocampal lysates from VGLUT3 −/− and C57BL/6 (WT) male mice. Data are the mean ± SEM ( n = 5–6 mice/group), normalized to the respective surface or total WT values. * p
    Anti Muscarinic Acetylcholine Receptor M1 Antibody Produced In Rabbit, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti muscarinic acetylcholine receptor m1 antibody produced in rabbit/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti muscarinic acetylcholine receptor m1 antibody produced in rabbit - by Bioz Stars, 2022-11
    93/100 stars
      Buy from Supplier

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    Millipore mouse anti flag m1 antibody
    The effect of VGLUT3 deletion on glutamate and nonglutamate receptors expression in the mouse hippocampus. A–G , Representative immunoblots and quantification of cell surface and total levels of mGluR5 ( A ), mGluR2/3 ( B ), NMDAR2A ( C ), NMDAR2B ( D ), GluA1 ( E ), D1R ( F ), and <t>M1</t> <t>mAChR</t> ( G ) in hippocampal lysates from VGLUT3 −/− and C57BL/6 (WT) male mice. Data are the mean ± SEM ( n = 5–6 mice/group), normalized to the respective surface or total WT values. * p
    Mouse Anti Flag M1 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti flag m1 antibody - by Bioz Stars, 2022-11
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      Buy from Supplier

    94
    Millipore rabbit antibody
    The effect of VGLUT3 deletion on glutamate and nonglutamate receptors expression in the mouse hippocampus. A–G , Representative immunoblots and quantification of cell surface and total levels of mGluR5 ( A ), mGluR2/3 ( B ), NMDAR2A ( C ), NMDAR2B ( D ), GluA1 ( E ), D1R ( F ), and <t>M1</t> <t>mAChR</t> ( G ) in hippocampal lysates from VGLUT3 −/− and C57BL/6 (WT) male mice. Data are the mean ± SEM ( n = 5–6 mice/group), normalized to the respective surface or total WT values. * p
    Rabbit Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit antibody/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit antibody - by Bioz Stars, 2022-11
    94/100 stars
      Buy from Supplier

    Image Search Results


    The effect of VGLUT3 deletion on glutamate and nonglutamate receptors expression in the mouse hippocampus. A–G , Representative immunoblots and quantification of cell surface and total levels of mGluR5 ( A ), mGluR2/3 ( B ), NMDAR2A ( C ), NMDAR2B ( D ), GluA1 ( E ), D1R ( F ), and M1 mAChR ( G ) in hippocampal lysates from VGLUT3 −/− and C57BL/6 (WT) male mice. Data are the mean ± SEM ( n = 5–6 mice/group), normalized to the respective surface or total WT values. * p

    Journal: eNeuro

    Article Title: VGLUT3 Ablation Differentially Modulates Glutamate Receptor Densities in Mouse Brain

    doi: 10.1523/ENEURO.0041-22.2022

    Figure Lengend Snippet: The effect of VGLUT3 deletion on glutamate and nonglutamate receptors expression in the mouse hippocampus. A–G , Representative immunoblots and quantification of cell surface and total levels of mGluR5 ( A ), mGluR2/3 ( B ), NMDAR2A ( C ), NMDAR2B ( D ), GluA1 ( E ), D1R ( F ), and M1 mAChR ( G ) in hippocampal lysates from VGLUT3 −/− and C57BL/6 (WT) male mice. Data are the mean ± SEM ( n = 5–6 mice/group), normalized to the respective surface or total WT values. * p

    Article Snippet: Rabbit anti-mGluR5 (catalog #AB5675), anti-NMDAR2A (catalog #AB1555), anti-NMDAR2B (catalog #AB1557), anti-AMPA1 (catalog #AB1504), and anti-M1 mAChR (catalog #M9808) were from Sigma-Aldrich.

    Techniques: Expressing, Western Blot, Mouse Assay

    The effect of VGLUT3 deletion on glutamate and nonglutamate receptor expression in the mouse cerebral cortex. A–G , Representative immunoblots and quantification of cell surface and total levels of mGluR5 ( A ), mGluR2/3 ( B ), NMDAR2A ( C ), NMDAR2B ( D ), GluA1 ( E ), D1R ( F ), and M1 mAChR ( G ) in cortical lysates from VGLUT3 −/− and C57BL/6 (WT) male mice. Data are the mean ± SEM ( n = 5–6 mice/group), normalized to the respective surface or total WT values. * p

    Journal: eNeuro

    Article Title: VGLUT3 Ablation Differentially Modulates Glutamate Receptor Densities in Mouse Brain

    doi: 10.1523/ENEURO.0041-22.2022

    Figure Lengend Snippet: The effect of VGLUT3 deletion on glutamate and nonglutamate receptor expression in the mouse cerebral cortex. A–G , Representative immunoblots and quantification of cell surface and total levels of mGluR5 ( A ), mGluR2/3 ( B ), NMDAR2A ( C ), NMDAR2B ( D ), GluA1 ( E ), D1R ( F ), and M1 mAChR ( G ) in cortical lysates from VGLUT3 −/− and C57BL/6 (WT) male mice. Data are the mean ± SEM ( n = 5–6 mice/group), normalized to the respective surface or total WT values. * p

    Article Snippet: Rabbit anti-mGluR5 (catalog #AB5675), anti-NMDAR2A (catalog #AB1555), anti-NMDAR2B (catalog #AB1557), anti-AMPA1 (catalog #AB1504), and anti-M1 mAChR (catalog #M9808) were from Sigma-Aldrich.

    Techniques: Expressing, Western Blot, Mouse Assay

    The effect of VGLUT3 deletion on glutamate and nonglutamate receptor expression in the mouse striatum. A–G , Representative immunoblots and quantification of cell surface and total levels of mGluR5 ( A ), mGluR2/3 ( B ), NMDAR2A ( C ), NMDAR2B ( D ), GluA1 ( E ), D1R ( F ), and M1 mAChR ( G ) in striatal lysates from VGLUT3 −/− and C57BL/6 (WT) male mice. Data are the mean ± SEM ( n = 5–6 mice/group), normalized to the respective surface or total WT values. * p

    Journal: eNeuro

    Article Title: VGLUT3 Ablation Differentially Modulates Glutamate Receptor Densities in Mouse Brain

    doi: 10.1523/ENEURO.0041-22.2022

    Figure Lengend Snippet: The effect of VGLUT3 deletion on glutamate and nonglutamate receptor expression in the mouse striatum. A–G , Representative immunoblots and quantification of cell surface and total levels of mGluR5 ( A ), mGluR2/3 ( B ), NMDAR2A ( C ), NMDAR2B ( D ), GluA1 ( E ), D1R ( F ), and M1 mAChR ( G ) in striatal lysates from VGLUT3 −/− and C57BL/6 (WT) male mice. Data are the mean ± SEM ( n = 5–6 mice/group), normalized to the respective surface or total WT values. * p

    Article Snippet: Rabbit anti-mGluR5 (catalog #AB5675), anti-NMDAR2A (catalog #AB1555), anti-NMDAR2B (catalog #AB1557), anti-AMPA1 (catalog #AB1504), and anti-M1 mAChR (catalog #M9808) were from Sigma-Aldrich.

    Techniques: Expressing, Western Blot, Mouse Assay