Journal: Nature Communications

Article Title: Neural representation of cytokines by vagal sensory neurons

doi: 10.1038/s41467-025-59248-6

Figure Lengend Snippet: a Representative field of view showing neurons that respond to specific cytokines. b Representative traces from individual sensory neurons responding to specific cytokines. c Heatmap demonstrating selective cytokine‐specific responses in normalized neural activity index. White dotted lines indicate the time of cytokine application on the vagus nerve. d Pie chart depicting the proportion of total responsive neurons to specific cytokines, multiple cytokines or nonspecific activity. e Representative confocal microscopy images showing antibody labeling of IL‐1R1 (red) and TNFR1 (green) cytokine receptors on nodose ganglia cell bodies. Scale bar, 50 µm; Zoomed inset scale bar, 25 µm. IHC was repeated independently in 5 mice, quantified over 2 sections each. f Quantification of nodose ganglion cell bodies labeled for IL‐1R1 and TNFR1.

Article Snippet: Tissues were incubated with the following primary antibodies diluted in blocking buffer overnight at 4 °C: rabbit IL1RA (Abcam, Ab124962), mouse TNFR1 or rabbit TNFR1 (Proteintech, 60192-1-Ig), rat IL10RA (Abcam, Ab33738) or rabbit IL10RA (ThermoFisher, PA5-109852), PHOX2B (Abcam, Ab183741) or rabbit PHOX2B Alexa Fluor 647 (Abcam, Ab311130), Rabbit PRDM12 (EMD Millipore, ABE95), and Chicken Anti-GFP (Aves Labs, GFP-1010).

Techniques: Activity Assay, Confocal Microscopy, Antibody Labeling, Labeling