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Revvity Signals pe anti mouse p2x7r antibody
Pe Anti Mouse P2x7r Antibody, supplied by Revvity Signals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Revvity Signals pe anti mouse p2x7r antibody
Pe Anti Mouse P2x7r Antibody, supplied by Revvity Signals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Structured Review

Revvity Signals pe anti mouse p2x7r antibody
Pe Anti Mouse P2x7r Antibody, supplied by Revvity Signals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology pe anti mouse p2x7r antibody
Pe Anti Mouse P2x7r Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Revvity Signals pe anti mouse p2x7r antibody
Pe Anti Mouse P2x7r Antibody, supplied by Revvity Signals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Revvity Signals pe anti mouse p2x7r
Pe Anti Mouse P2x7r, supplied by Revvity Signals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Revvity Signals pe anti mouse p2x7r
Pe Anti Mouse P2x7r, supplied by Revvity Signals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Atlas Antibodies monoclonal mouse anti human p2x7r antibody
Monoclonal Mouse Anti Human P2x7r Antibody, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Atlas Antibodies monoclonal mouse anti human p2x7r antibody
P2X1, P2X4, and <t>P2X7</t> <t>receptor</t> expression in muscle-invasive bladder cancer (MIBC). ( A – C ) Representative photomicrographs show TMA samples with low ( A ) and high ( B ) P2X1R expression in cancer cells of MIBC. Compared to smooth muscle cells, muscle infiltrating cancer cells in ( C ) show strong distinct membranous staining. ( D – F ) TMA samples of MIBC with low ( D ) and high ( E ) P2X4R expression are shown. MIBC cells exhibit membranous and partially fine-granular cytoplasmic P2X4R staining, while tumor-infiltrating immune cells show strong immunoreactivity ( F ). ( G – I ) Low and high <t>P2X7R</t> expression patterns in MIBC are shown in ( G ) and ( H ), respectively. Cancer cells between muscle cells and in lymphatic vessels are highlighted by strong membranous P2X7R expression ( I ). Scale bars equal 100 μm (outer pictures and ( C , F , I )) and 20 μm (inserts).
Monoclonal Mouse Anti Human P2x7r Antibody, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "P2X1 and P2X7 Receptor Overexpression Is a Negative Predictor of Survival in Muscle-Invasive Bladder Cancer"

Article Title: P2X1 and P2X7 Receptor Overexpression Is a Negative Predictor of Survival in Muscle-Invasive Bladder Cancer

Journal: Cancers

doi: 10.3390/cancers15082321

P2X1, P2X4, and P2X7 receptor expression in muscle-invasive bladder cancer (MIBC). ( A – C ) Representative photomicrographs show TMA samples with low ( A ) and high ( B ) P2X1R expression in cancer cells of MIBC. Compared to smooth muscle cells, muscle infiltrating cancer cells in ( C ) show strong distinct membranous staining. ( D – F ) TMA samples of MIBC with low ( D ) and high ( E ) P2X4R expression are shown. MIBC cells exhibit membranous and partially fine-granular cytoplasmic P2X4R staining, while tumor-infiltrating immune cells show strong immunoreactivity ( F ). ( G – I ) Low and high P2X7R expression patterns in MIBC are shown in ( G ) and ( H ), respectively. Cancer cells between muscle cells and in lymphatic vessels are highlighted by strong membranous P2X7R expression ( I ). Scale bars equal 100 μm (outer pictures and ( C , F , I )) and 20 μm (inserts).
Figure Legend Snippet: P2X1, P2X4, and P2X7 receptor expression in muscle-invasive bladder cancer (MIBC). ( A – C ) Representative photomicrographs show TMA samples with low ( A ) and high ( B ) P2X1R expression in cancer cells of MIBC. Compared to smooth muscle cells, muscle infiltrating cancer cells in ( C ) show strong distinct membranous staining. ( D – F ) TMA samples of MIBC with low ( D ) and high ( E ) P2X4R expression are shown. MIBC cells exhibit membranous and partially fine-granular cytoplasmic P2X4R staining, while tumor-infiltrating immune cells show strong immunoreactivity ( F ). ( G – I ) Low and high P2X7R expression patterns in MIBC are shown in ( G ) and ( H ), respectively. Cancer cells between muscle cells and in lymphatic vessels are highlighted by strong membranous P2X7R expression ( I ). Scale bars equal 100 μm (outer pictures and ( C , F , I )) and 20 μm (inserts).

Techniques Used: Expressing, Staining

Demographics, clinicopathological characteristics, and associations with P2X1, P2X4, and  P2X7 receptor  expression.
Figure Legend Snippet: Demographics, clinicopathological characteristics, and associations with P2X1, P2X4, and P2X7 receptor expression.

Techniques Used: Expressing

Effect of P2X receptor expression levels on overall survival in muscle-invasive bladder cancer (MIBC). Expression of P2X1R ( A ), P2X4R ( B ), P2X7R ( C ), and combined expression of P2X1R and P2X7R ( D ) was immunohistochemically analyzed in MIBC and scored as “low” (below median) or “high” (above median). Kaplan–Meier overall survival (OS) curves were plotted, and groups were compared by the log-rank test; ( A ) and ( B ): n = 171; ( C ): n = 172; ( D ): n = 170.
Figure Legend Snippet: Effect of P2X receptor expression levels on overall survival in muscle-invasive bladder cancer (MIBC). Expression of P2X1R ( A ), P2X4R ( B ), P2X7R ( C ), and combined expression of P2X1R and P2X7R ( D ) was immunohistochemically analyzed in MIBC and scored as “low” (below median) or “high” (above median). Kaplan–Meier overall survival (OS) curves were plotted, and groups were compared by the log-rank test; ( A ) and ( B ): n = 171; ( C ): n = 172; ( D ): n = 170.

Techniques Used: Expressing

Effect of P2X receptor expression levels on tumor-specific survival (TSS) in muscle-invasive bladder cancer (MIBC). Expression of P2X1R ( A ), P2X4R ( B ), P2X7R ( C ), and combined expression of P2X1R and P2X7R ( D ) was immunohistochemically analyzed in MIBC and scored as “low” (below median) or “high” (above median). Kaplan–Meier tumor-specific survival (TSS) curves were plotted, and groups were compared by the log-rank test; ( A , B ): n = 171; ( C ): n = 172; ( D ): n = 170.
Figure Legend Snippet: Effect of P2X receptor expression levels on tumor-specific survival (TSS) in muscle-invasive bladder cancer (MIBC). Expression of P2X1R ( A ), P2X4R ( B ), P2X7R ( C ), and combined expression of P2X1R and P2X7R ( D ) was immunohistochemically analyzed in MIBC and scored as “low” (below median) or “high” (above median). Kaplan–Meier tumor-specific survival (TSS) curves were plotted, and groups were compared by the log-rank test; ( A , B ): n = 171; ( C ): n = 172; ( D ): n = 170.

Techniques Used: Expressing

Effect of P2X receptor expression levels on disease-free survival (DFS) in muscle-invasive bladder cancer (MIBC). Expression of P2X1R ( A ), P2X4R ( B ), P2X7R ( C ), and combined expression of P2X1R and P2X7R ( D ) was immunohistochemically analyzed in MIBC and scored as “low” (below median) or “high” (above median). Kaplan–Meier disease-free survival (DFS) curves were plotted, and groups were compared by the log-rank test; ( A , B ): n = 171; ( C ): n = 172; ( D ): n = 170.
Figure Legend Snippet: Effect of P2X receptor expression levels on disease-free survival (DFS) in muscle-invasive bladder cancer (MIBC). Expression of P2X1R ( A ), P2X4R ( B ), P2X7R ( C ), and combined expression of P2X1R and P2X7R ( D ) was immunohistochemically analyzed in MIBC and scored as “low” (below median) or “high” (above median). Kaplan–Meier disease-free survival (DFS) curves were plotted, and groups were compared by the log-rank test; ( A , B ): n = 171; ( C ): n = 172; ( D ): n = 170.

Techniques Used: Expressing

Multivariate analysis of potential prognostic survival factors in MIBC patients.
Figure Legend Snippet: Multivariate analysis of potential prognostic survival factors in MIBC patients.

Techniques Used:


Structured Review

PerkinElmer p2x7r anti mouse pe
A, B BMDM were stimulated for 6 h (A) or 12 h (B) with Poly(I:C) (50 μg/ml) or LPS (100 ng/ml) cell surface <t>P2X7R</t> measured by flow cytometry. MFI were normalised to the highest value, considered as 100, to allow pooling of independent experiments (A, pooled results from three independent experiments, WT mice = 3, Calhm6 −/− mice = 3) (B, pooled results from two independent experiments, WT mice = 2, Calhm6 −/− mice = 2). C–E Percentage of cell death measured with LDH assay after inflammasome activation in BMDM: BMDM were treated with Poly(I:C) (50 μg/ml) for 2 h followed by 1 h of ATP (2.5 mM), or nigericin (5 μM) stimulation (C, Representative experiment shown, WT mice = 2, Calhm6 −/− mice = 2) (D, pooled results from two independent experiments, WT mice = 4, Calhm6 −/− mice = 4). (E) BMDM were stimulated with LPS (100 ng/ml) for 5 h in total and nigericin (5 μM) was added 1 h before harvest (pooled results from three independent experiments, WT mice = 3, Calhm6 −/− mice = 3). Data information: Test = Kruskal–Wallis test with multiple comparisons, error bars represent SD.
P2x7r Anti Mouse Pe, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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p2x7r anti mouse pe - by Bioz Stars, 2024-07
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Images

1) Product Images from "The ion channel CALHM6 controls bacterial infection‐induced cellular cross‐talk at the immunological synapse"

Article Title: The ion channel CALHM6 controls bacterial infection‐induced cellular cross‐talk at the immunological synapse

Journal: The EMBO Journal

doi: 10.15252/embj.2022111450

A, B BMDM were stimulated for 6 h (A) or 12 h (B) with Poly(I:C) (50 μg/ml) or LPS (100 ng/ml) cell surface P2X7R measured by flow cytometry. MFI were normalised to the highest value, considered as 100, to allow pooling of independent experiments (A, pooled results from three independent experiments, WT mice = 3, Calhm6 −/− mice = 3) (B, pooled results from two independent experiments, WT mice = 2, Calhm6 −/− mice = 2). C–E Percentage of cell death measured with LDH assay after inflammasome activation in BMDM: BMDM were treated with Poly(I:C) (50 μg/ml) for 2 h followed by 1 h of ATP (2.5 mM), or nigericin (5 μM) stimulation (C, Representative experiment shown, WT mice = 2, Calhm6 −/− mice = 2) (D, pooled results from two independent experiments, WT mice = 4, Calhm6 −/− mice = 4). (E) BMDM were stimulated with LPS (100 ng/ml) for 5 h in total and nigericin (5 μM) was added 1 h before harvest (pooled results from three independent experiments, WT mice = 3, Calhm6 −/− mice = 3). Data information: Test = Kruskal–Wallis test with multiple comparisons, error bars represent SD.
Figure Legend Snippet: A, B BMDM were stimulated for 6 h (A) or 12 h (B) with Poly(I:C) (50 μg/ml) or LPS (100 ng/ml) cell surface P2X7R measured by flow cytometry. MFI were normalised to the highest value, considered as 100, to allow pooling of independent experiments (A, pooled results from three independent experiments, WT mice = 3, Calhm6 −/− mice = 3) (B, pooled results from two independent experiments, WT mice = 2, Calhm6 −/− mice = 2). C–E Percentage of cell death measured with LDH assay after inflammasome activation in BMDM: BMDM were treated with Poly(I:C) (50 μg/ml) for 2 h followed by 1 h of ATP (2.5 mM), or nigericin (5 μM) stimulation (C, Representative experiment shown, WT mice = 2, Calhm6 −/− mice = 2) (D, pooled results from two independent experiments, WT mice = 4, Calhm6 −/− mice = 4). (E) BMDM were stimulated with LPS (100 ng/ml) for 5 h in total and nigericin (5 μM) was added 1 h before harvest (pooled results from three independent experiments, WT mice = 3, Calhm6 −/− mice = 3). Data information: Test = Kruskal–Wallis test with multiple comparisons, error bars represent SD.

Techniques Used: Flow Cytometry, Lactate Dehydrogenase Assay, Activation Assay


Figure Legend Snippet:

Techniques Used: Western Blot, Protease Inhibitor, Sample Prep, Flow Cytometry, Staining, Purification, Microscopy, Recombinant, Activation Assay, Transfection, Transduction, Enzyme-linked Immunosorbent Assay, Cytotoxicity Assay, Lactate Dehydrogenase Assay, Selection, Plasmid Preparation, ATP Bioluminescent Assay, SYBR Green Assay, Gel Extraction, Generated, Expressing, Sequencing, Software

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    Revvity Signals pe anti mouse p2x7r antibody
    Pe Anti Mouse P2x7r Antibody, supplied by Revvity Signals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe anti mouse p2x7r antibody/product/Revvity Signals
    Average 86 stars, based on 1 article reviews
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    pe anti mouse p2x7r antibody - by Bioz Stars, 2024-07
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    Santa Cruz Biotechnology pe anti mouse p2x7r antibody
    Pe Anti Mouse P2x7r Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe anti mouse p2x7r antibody/product/Santa Cruz Biotechnology
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    pe anti mouse p2x7r antibody - by Bioz Stars, 2024-07
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    Revvity Signals pe anti mouse p2x7r
    Pe Anti Mouse P2x7r, supplied by Revvity Signals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe anti mouse p2x7r/product/Revvity Signals
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    Atlas Antibodies monoclonal mouse anti human p2x7r antibody
    Monoclonal Mouse Anti Human P2x7r Antibody, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal mouse anti human p2x7r antibody/product/Atlas Antibodies
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    monoclonal mouse anti human p2x7r antibody - by Bioz Stars, 2024-07
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    PerkinElmer p2x7r anti mouse pe
    A, B BMDM were stimulated for 6 h (A) or 12 h (B) with Poly(I:C) (50 μg/ml) or LPS (100 ng/ml) cell surface <t>P2X7R</t> measured by flow cytometry. MFI were normalised to the highest value, considered as 100, to allow pooling of independent experiments (A, pooled results from three independent experiments, WT mice = 3, Calhm6 −/− mice = 3) (B, pooled results from two independent experiments, WT mice = 2, Calhm6 −/− mice = 2). C–E Percentage of cell death measured with LDH assay after inflammasome activation in BMDM: BMDM were treated with Poly(I:C) (50 μg/ml) for 2 h followed by 1 h of ATP (2.5 mM), or nigericin (5 μM) stimulation (C, Representative experiment shown, WT mice = 2, Calhm6 −/− mice = 2) (D, pooled results from two independent experiments, WT mice = 4, Calhm6 −/− mice = 4). (E) BMDM were stimulated with LPS (100 ng/ml) for 5 h in total and nigericin (5 μM) was added 1 h before harvest (pooled results from three independent experiments, WT mice = 3, Calhm6 −/− mice = 3). Data information: Test = Kruskal–Wallis test with multiple comparisons, error bars represent SD.
    P2x7r Anti Mouse Pe, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2x7r anti mouse pe/product/PerkinElmer
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    p2x7r anti mouse pe - by Bioz Stars, 2024-07
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    A, B BMDM were stimulated for 6 h (A) or 12 h (B) with Poly(I:C) (50 μg/ml) or LPS (100 ng/ml) cell surface P2X7R measured by flow cytometry. MFI were normalised to the highest value, considered as 100, to allow pooling of independent experiments (A, pooled results from three independent experiments, WT mice = 3, Calhm6 −/− mice = 3) (B, pooled results from two independent experiments, WT mice = 2, Calhm6 −/− mice = 2). C–E Percentage of cell death measured with LDH assay after inflammasome activation in BMDM: BMDM were treated with Poly(I:C) (50 μg/ml) for 2 h followed by 1 h of ATP (2.5 mM), or nigericin (5 μM) stimulation (C, Representative experiment shown, WT mice = 2, Calhm6 −/− mice = 2) (D, pooled results from two independent experiments, WT mice = 4, Calhm6 −/− mice = 4). (E) BMDM were stimulated with LPS (100 ng/ml) for 5 h in total and nigericin (5 μM) was added 1 h before harvest (pooled results from three independent experiments, WT mice = 3, Calhm6 −/− mice = 3). Data information: Test = Kruskal–Wallis test with multiple comparisons, error bars represent SD.

    Journal: The EMBO Journal

    Article Title: The ion channel CALHM6 controls bacterial infection‐induced cellular cross‐talk at the immunological synapse

    doi: 10.15252/embj.2022111450

    Figure Lengend Snippet: A, B BMDM were stimulated for 6 h (A) or 12 h (B) with Poly(I:C) (50 μg/ml) or LPS (100 ng/ml) cell surface P2X7R measured by flow cytometry. MFI were normalised to the highest value, considered as 100, to allow pooling of independent experiments (A, pooled results from three independent experiments, WT mice = 3, Calhm6 −/− mice = 3) (B, pooled results from two independent experiments, WT mice = 2, Calhm6 −/− mice = 2). C–E Percentage of cell death measured with LDH assay after inflammasome activation in BMDM: BMDM were treated with Poly(I:C) (50 μg/ml) for 2 h followed by 1 h of ATP (2.5 mM), or nigericin (5 μM) stimulation (C, Representative experiment shown, WT mice = 2, Calhm6 −/− mice = 2) (D, pooled results from two independent experiments, WT mice = 4, Calhm6 −/− mice = 4). (E) BMDM were stimulated with LPS (100 ng/ml) for 5 h in total and nigericin (5 μM) was added 1 h before harvest (pooled results from three independent experiments, WT mice = 3, Calhm6 −/− mice = 3). Data information: Test = Kruskal–Wallis test with multiple comparisons, error bars represent SD.

    Article Snippet: P2X7R anti‐mouse PE , BioLegend , 148703 RRID:AB_2650951.

    Techniques: Flow Cytometry, Lactate Dehydrogenase Assay, Activation Assay

    Journal: The EMBO Journal

    Article Title: The ion channel CALHM6 controls bacterial infection‐induced cellular cross‐talk at the immunological synapse

    doi: 10.15252/embj.2022111450

    Figure Lengend Snippet:

    Article Snippet: P2X7R anti‐mouse PE , BioLegend , 148703 RRID:AB_2650951.

    Techniques: Western Blot, Protease Inhibitor, Sample Prep, Flow Cytometry, Staining, Purification, Microscopy, Recombinant, Activation Assay, Transfection, Transduction, Enzyme-linked Immunosorbent Assay, Cytotoxicity Assay, Lactate Dehydrogenase Assay, Selection, Plasmid Preparation, ATP Bioluminescent Assay, SYBR Green Assay, Gel Extraction, Generated, Expressing, Sequencing, Software