monoclonal anti human igg3 biotin antibody  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Name:
    Monoclonal Anti Human IgG2 Biotin antibody
    Description:

    Catalog Number:
    b3398
    Price:
    None
    Applications:
    Monoclonal Anti-Human IgG2-Biotin antibody produced in mouse was used to quantify human IgG1 by isotype-specific immunoblotting.
    Buy from Supplier


    Structured Review

    Millipore monoclonal anti human igg3 biotin antibody
    Graphic representation showing correlation coefficients obtained by correlating the string of reactivity of serum <t>IgG</t> ofdifferent individuals to different microorganism, obtained in ELISA, at steady state. A) Professional athletes; B) Control group. Red colour–Pearson product-moment correlation coefficient, r = 1, Blue– r = -1.

    https://www.bioz.com/result/monoclonal anti human igg3 biotin antibody/product/Millipore
    Average 93 stars, based on 1684 article reviews
    Price from $9.99 to $1999.99
    monoclonal anti human igg3 biotin antibody - by Bioz Stars, 2020-07
    93/100 stars

    Images

    1) Product Images from "Profiling of microorganism-binding serum antibody specificities in professional athletes"

    Article Title: Profiling of microorganism-binding serum antibody specificities in professional athletes

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0203665

    Graphic representation showing correlation coefficients obtained by correlating the string of reactivity of serum IgG ofdifferent individuals to different microorganism, obtained in ELISA, at steady state. A) Professional athletes; B) Control group. Red colour–Pearson product-moment correlation coefficient, r = 1, Blue– r = -1.
    Figure Legend Snippet: Graphic representation showing correlation coefficients obtained by correlating the string of reactivity of serum IgG ofdifferent individuals to different microorganism, obtained in ELISA, at steady state. A) Professional athletes; B) Control group. Red colour–Pearson product-moment correlation coefficient, r = 1, Blue– r = -1.

    Techniques Used: Enzyme-linked Immunosorbent Assay

    Graphic representation showing correlation coefficients obtained by correlating the string of reactivity of serumtotal IgG and total IgA with IgG subclasses, for different microorganisms in professional athletes. A) Escherichia coli ATCC25922; B) Candida albicans ATCC 10259; C) Lactobacillus plantarum WCFS1; D) Salmonella typhimurium 2865; E) Lactobacillusrhamnosus LGG, F) LPS from E . coli O55:B5.
    Figure Legend Snippet: Graphic representation showing correlation coefficients obtained by correlating the string of reactivity of serumtotal IgG and total IgA with IgG subclasses, for different microorganisms in professional athletes. A) Escherichia coli ATCC25922; B) Candida albicans ATCC 10259; C) Lactobacillus plantarum WCFS1; D) Salmonella typhimurium 2865; E) Lactobacillusrhamnosus LGG, F) LPS from E . coli O55:B5.

    Techniques Used:

    Analysis of reactivity of serial dilutions of serum IgG in A) professional athletes; B) control group, and of serum IgA in C) professional athletes; D) control group to different microorganisms. Red colour– E . coli ATCC25922; black - C . albicans ATCC 10259; turquoise– L . plantarum WCFS1; green– S . typhimurium 2865; blue— L . rhamnosus LGG, violet—LPS from E . coli 055:B5.
    Figure Legend Snippet: Analysis of reactivity of serial dilutions of serum IgG in A) professional athletes; B) control group, and of serum IgA in C) professional athletes; D) control group to different microorganisms. Red colour– E . coli ATCC25922; black - C . albicans ATCC 10259; turquoise– L . plantarum WCFS1; green– S . typhimurium 2865; blue— L . rhamnosus LGG, violet—LPS from E . coli 055:B5.

    Techniques Used:

    The strings of reactivities (apsorbance) of IgG and IgA to bacteria from different individuals were correlated, and the Pearson product-moment correlation coefficientswereplotted for individual bacteria. Red colour- Professional athletes PA; Green colour–Control.
    Figure Legend Snippet: The strings of reactivities (apsorbance) of IgG and IgA to bacteria from different individuals were correlated, and the Pearson product-moment correlation coefficientswereplotted for individual bacteria. Red colour- Professional athletes PA; Green colour–Control.

    Techniques Used:

    2) Product Images from "Carbohydrate Sequence of the Prostate Cancer-associated Antigen F77 Assigned by a Mucin O-Glycome Designer Array *"

    Article Title: Carbohydrate Sequence of the Prostate Cancer-associated Antigen F77 Assigned by a Mucin O-Glycome Designer Array *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M114.558932

    Detection of F77 antigen on PSM and on minor components among O -glycans released from the mucin. A, microarray analyses of mAbs F77, anti-B (89-F), anti-A (T36), and UEA-I lectin with mucin-type glycoproteins. The descriptions of the glycoproteins are in Table 1 . Results are the means of fluorescence intensities of duplicate spots printed at 150 pg of glycoprotein per spot. The error bars represent half of the difference between the two values. B, gel filtration chromatography of the products of reductive alkaline hydrolysis from PSM. Inset is the initial chromatography profile using a Bio-Gel P4 column (1.6 × 90 cm) eluted with H 2 O. The main panel shows the profile of fraction a from the Bio-Gel P4 column, chromatographed using a Bio-Gel P6 column (1.6 × 90 cm) eluted with H 2 O. V 0 is the void volume of the column; V t is the total volume; glucose units 8–11 indicate positions of elution of oligosaccharides with degrees of polymerization 8–11 in an acid hydrolysate of dextran. F1–F7 designate the pooled fractions that were converted to NGLs. C, binding of mAb F77 to NGLs derived from the O -glycans in fractions 1–7. The NGLs derived from the O -glycans in fractions 1–7 were chromatographed on silica gel HPTLC plates, using CHCl 3 /MeOH/H 2 O, 60:35:8 (v/v), as solvent. The fluorescent NGLs were visualized under UV light ( left panel ). The same plate and a duplicate plate were incubated with mAb F77 ( middle panel ) and the isotype IgG3 control, MG3-35 ( right panel ), followed by biotinylated anti-mouse immunoglobulins. Binding was detected as in Fig. 1 . O indicates the origin.
    Figure Legend Snippet: Detection of F77 antigen on PSM and on minor components among O -glycans released from the mucin. A, microarray analyses of mAbs F77, anti-B (89-F), anti-A (T36), and UEA-I lectin with mucin-type glycoproteins. The descriptions of the glycoproteins are in Table 1 . Results are the means of fluorescence intensities of duplicate spots printed at 150 pg of glycoprotein per spot. The error bars represent half of the difference between the two values. B, gel filtration chromatography of the products of reductive alkaline hydrolysis from PSM. Inset is the initial chromatography profile using a Bio-Gel P4 column (1.6 × 90 cm) eluted with H 2 O. The main panel shows the profile of fraction a from the Bio-Gel P4 column, chromatographed using a Bio-Gel P6 column (1.6 × 90 cm) eluted with H 2 O. V 0 is the void volume of the column; V t is the total volume; glucose units 8–11 indicate positions of elution of oligosaccharides with degrees of polymerization 8–11 in an acid hydrolysate of dextran. F1–F7 designate the pooled fractions that were converted to NGLs. C, binding of mAb F77 to NGLs derived from the O -glycans in fractions 1–7. The NGLs derived from the O -glycans in fractions 1–7 were chromatographed on silica gel HPTLC plates, using CHCl 3 /MeOH/H 2 O, 60:35:8 (v/v), as solvent. The fluorescent NGLs were visualized under UV light ( left panel ). The same plate and a duplicate plate were incubated with mAb F77 ( middle panel ) and the isotype IgG3 control, MG3-35 ( right panel ), followed by biotinylated anti-mouse immunoglobulins. Binding was detected as in Fig. 1 . O indicates the origin.

    Techniques Used: Microarray, Fluorescence, Filtration, Chromatography, Binding Assay, Derivative Assay, High Performance Thin Layer Chromatography, Incubation

    3) Product Images from "Increased Biodiversity in the Environment Improves the Humoral Response of Rats"

    Article Title: Increased Biodiversity in the Environment Improves the Humoral Response of Rats

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0120255

    Natural anti-human serum albumin antibody levels in the serum of biome depleted (n = 20) and biome enriched (n = 15) rats. The relative concentration of antibody was determined by ELISA as described in the Methods. Relative levels of (A) IgM and (B) IgG are shown. Binding to human serum albumin (HSA) was used as a measure of reactivity toward a xenogeneic antigen for which the animals lacked previous exposure. The means, standard errors, and the p -values associated with comparing data from biome depleted and biome enriched animals using a t-test are shown. (NS = not significant)
    Figure Legend Snippet: Natural anti-human serum albumin antibody levels in the serum of biome depleted (n = 20) and biome enriched (n = 15) rats. The relative concentration of antibody was determined by ELISA as described in the Methods. Relative levels of (A) IgM and (B) IgG are shown. Binding to human serum albumin (HSA) was used as a measure of reactivity toward a xenogeneic antigen for which the animals lacked previous exposure. The means, standard errors, and the p -values associated with comparing data from biome depleted and biome enriched animals using a t-test are shown. (NS = not significant)

    Techniques Used: Concentration Assay, Enzyme-linked Immunosorbent Assay, Binding Assay

    4) Product Images from "Contributions of Edema Factor and Protective Antigen to the Induction of Protective Immunity by Bacillus anthracis Edema Toxin as an Intranasal Adjuvant"

    Article Title: Contributions of Edema Factor and Protective Antigen to the Induction of Protective Immunity by Bacillus anthracis Edema Toxin as an Intranasal Adjuvant

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.0902795

    F1-V–specific Abs after intranasal immunization with wt PA andEF mutants. A, F1-V–specific serum Ab isotype and IgG subclass responsesafter intranasal administration of EdTx derivatives. Mice were immunized threetimes at weekly intervals
    Figure Legend Snippet: F1-V–specific Abs after intranasal immunization with wt PA andEF mutants. A, F1-V–specific serum Ab isotype and IgG subclass responsesafter intranasal administration of EdTx derivatives. Mice were immunized threetimes at weekly intervals

    Techniques Used: Mouse Assay

    PA-specific Abs after intranasal immunization with PA mutants and EFwt. A , Serum PA-specific Ab isotype and IgG subclass responsesafter intranasal administration of EdTx derivatives. Mice were immunized threetimes at weekly intervals with 2 µg
    Figure Legend Snippet: PA-specific Abs after intranasal immunization with PA mutants and EFwt. A , Serum PA-specific Ab isotype and IgG subclass responsesafter intranasal administration of EdTx derivatives. Mice were immunized threetimes at weekly intervals with 2 µg

    Techniques Used: Mouse Assay

    PA-specific Abs after intranasal immunization with wt PA and EF mutants. A , PA-specific serum Ab isotype and IgG subclass responsesafter intranasal administration of EdTx derivatives. Mice were immunized threetimes at weekly intervals with 2 µg
    Figure Legend Snippet: PA-specific Abs after intranasal immunization with wt PA and EF mutants. A , PA-specific serum Ab isotype and IgG subclass responsesafter intranasal administration of EdTx derivatives. Mice were immunized threetimes at weekly intervals with 2 µg

    Techniques Used: Mouse Assay

    5) Product Images from "Optimization of incubation conditions of Plasmodium falciparum antibody multiplex assays to measure IgG, IgG1–4, IgM and IgE using standard and customized reference pools for sero-epidemiological and vaccine studies"

    Article Title: Optimization of incubation conditions of Plasmodium falciparum antibody multiplex assays to measure IgG, IgG1–4, IgM and IgE using standard and customized reference pools for sero-epidemiological and vaccine studies

    Journal: Malaria Journal

    doi: 10.1186/s12936-018-2369-3

    RTS,S-specific responses measured in the WHO reference reagent, IgM pool and samples from RTS,S-vaccinated children. The 3 samples from RTS,S vaccinated children were of high, medium and low CSP IgG titres. a – g IgG, IgG 1–4 , IgM and IgE levels to RTS,S-specific antigens measured in the WHO reference reagent; IgG, IgG 1, IgG2 and IgG4 also measured in RTS,S-vaccinated children; h IgM levels to RTS,S-specific antigens measured in the IgM pool vs. RTS,S-vaccinated children. The plots represent the levels of antibodies measured in serial dilutions of the positive pools (1:3 starting at 1:50 for IgG, IgG 1–4 and IgM; and 1:2 starting at 1:10 for IgE), and the RTS,S vaccinees samples (1:10 starting at 1:500 for IgG, 1:100 for IgM, 1:50 for IgG 1–4 ; and 1:2 starting at 1:10 for IgE). Isolated dots represent the levels measured in the technical blanks
    Figure Legend Snippet: RTS,S-specific responses measured in the WHO reference reagent, IgM pool and samples from RTS,S-vaccinated children. The 3 samples from RTS,S vaccinated children were of high, medium and low CSP IgG titres. a – g IgG, IgG 1–4 , IgM and IgE levels to RTS,S-specific antigens measured in the WHO reference reagent; IgG, IgG 1, IgG2 and IgG4 also measured in RTS,S-vaccinated children; h IgM levels to RTS,S-specific antigens measured in the IgM pool vs. RTS,S-vaccinated children. The plots represent the levels of antibodies measured in serial dilutions of the positive pools (1:3 starting at 1:50 for IgG, IgG 1–4 and IgM; and 1:2 starting at 1:10 for IgE), and the RTS,S vaccinees samples (1:10 starting at 1:500 for IgG, 1:100 for IgM, 1:50 for IgG 1–4 ; and 1:2 starting at 1:10 for IgE). Isolated dots represent the levels measured in the technical blanks

    Techniques Used: Isolation

    IgG, IgG 1–4 and IgM fitted curves using the WHO-CSP pool to the 40-antigen multiplex panel incubating at 4 °C ON. Lines and dots represent predicted levels from 5PL, 4PL or exponential regression equations from 23 titration curves for IgG, IgG1, IgG3 and IgM; and 12 curves for IgG2 and IgG4. Titration curves contained 18 serial dilutions (1:2) starting at 1/50 of the WHO-CSP pool to a panel of 39 P. falciparum antigens plus HBsAg, α-Gal, BSA and GST
    Figure Legend Snippet: IgG, IgG 1–4 and IgM fitted curves using the WHO-CSP pool to the 40-antigen multiplex panel incubating at 4 °C ON. Lines and dots represent predicted levels from 5PL, 4PL or exponential regression equations from 23 titration curves for IgG, IgG1, IgG3 and IgM; and 12 curves for IgG2 and IgG4. Titration curves contained 18 serial dilutions (1:2) starting at 1/50 of the WHO-CSP pool to a panel of 39 P. falciparum antigens plus HBsAg, α-Gal, BSA and GST

    Techniques Used: Multiplex Assay, Titration

    6) Product Images from "Increased Biodiversity in the Environment Improves the Humoral Response of Rats"

    Article Title: Increased Biodiversity in the Environment Improves the Humoral Response of Rats

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0120255

    Natural anti-human serum albumin antibody levels in the serum of biome depleted (n = 20) and biome enriched (n = 15) rats. The relative concentration of antibody was determined by ELISA as described in the Methods. Relative levels of (A) IgM and (B) IgG are shown. Binding to human serum albumin (HSA) was used as a measure of reactivity toward a xenogeneic antigen for which the animals lacked previous exposure. The means, standard errors, and the p -values associated with comparing data from biome depleted and biome enriched animals using a t-test are shown. (NS = not significant)
    Figure Legend Snippet: Natural anti-human serum albumin antibody levels in the serum of biome depleted (n = 20) and biome enriched (n = 15) rats. The relative concentration of antibody was determined by ELISA as described in the Methods. Relative levels of (A) IgM and (B) IgG are shown. Binding to human serum albumin (HSA) was used as a measure of reactivity toward a xenogeneic antigen for which the animals lacked previous exposure. The means, standard errors, and the p -values associated with comparing data from biome depleted and biome enriched animals using a t-test are shown. (NS = not significant)

    Techniques Used: Concentration Assay, Enzyme-linked Immunosorbent Assay, Binding Assay

    Relative concentration of DNP-specific antibody in the serum of biome depleted (n = 20) and biome enriched (n = 15) rats. The relative concentration of antibody was determined by ELISA as described in the Methods. Relative levels of (A) IgM, (B) IgG, and (C) subclasses of IgG are shown. The means and standard errors are shown. The p -values associated with comparing data from biome depleted and biome enriched animals using a t-test are shown. (NS = not significant)
    Figure Legend Snippet: Relative concentration of DNP-specific antibody in the serum of biome depleted (n = 20) and biome enriched (n = 15) rats. The relative concentration of antibody was determined by ELISA as described in the Methods. Relative levels of (A) IgM, (B) IgG, and (C) subclasses of IgG are shown. The means and standard errors are shown. The p -values associated with comparing data from biome depleted and biome enriched animals using a t-test are shown. (NS = not significant)

    Techniques Used: Concentration Assay, Enzyme-linked Immunosorbent Assay

    7) Product Images from "Peptide mimotopes of malondialdehyde epitopes for clinical applications in cardiovascular disease [S]"

    Article Title: Peptide mimotopes of malondialdehyde epitopes for clinical applications in cardiovascular disease [S]

    Journal: Journal of Lipid Research

    doi: 10.1194/jlr.M025445

    MDA mimotope immunization induces MDA-specific Ab responses. (A, B) ELISA for the binding of plasma Abs of immunized mice to MDA-LDL, MAA-LDL, and P2. C57BL/6 mice were immunized with P2-BSA (n = 3) or BSA (n = 3) as described in Materials and Methods. Dilution curves of IgG1 and IgM binding to indicated antigens in pre- and postimmune plasma was determined by chemiluminescent ELISA. (A) Dilution curve of IgG1 binding. (B) Dilution curve of IgM binding. Values are given as relative light units (RLU) per 100 ms and represent the mean ± SEM of each group. (C) Immunohistochemical staining of human carotid endarterectomy specimens. Sections were stained with pooled preimmune (A) or postimmune plasma (B) of P2-BSA-immunized mice or with the MDA-LDL-specific mAb MDA2 (C). Positive staining is indicated by red color, and nuclei are counterstained with hematoxylin.
    Figure Legend Snippet: MDA mimotope immunization induces MDA-specific Ab responses. (A, B) ELISA for the binding of plasma Abs of immunized mice to MDA-LDL, MAA-LDL, and P2. C57BL/6 mice were immunized with P2-BSA (n = 3) or BSA (n = 3) as described in Materials and Methods. Dilution curves of IgG1 and IgM binding to indicated antigens in pre- and postimmune plasma was determined by chemiluminescent ELISA. (A) Dilution curve of IgG1 binding. (B) Dilution curve of IgM binding. Values are given as relative light units (RLU) per 100 ms and represent the mean ± SEM of each group. (C) Immunohistochemical staining of human carotid endarterectomy specimens. Sections were stained with pooled preimmune (A) or postimmune plasma (B) of P2-BSA-immunized mice or with the MDA-LDL-specific mAb MDA2 (C). Positive staining is indicated by red color, and nuclei are counterstained with hematoxylin.

    Techniques Used: Multiple Displacement Amplification, Enzyme-linked Immunosorbent Assay, Binding Assay, Mouse Assay, Chemiluminescent ELISA, Mass Spectrometry, Immunohistochemistry, Staining

    Mimotopes are recognized by human autoAbs and mimic MDA-LDL. (A–D) Correlation of mimotope and MDA-LDL-specific Ab titers in human plasma. Plasma of middle-aged healthy volunteers (n = 18) was obtained in a previous study ( 35 ) and Ab titers to P1, P2, and MDA-LDL were measured at 1:400 dilution by chemiluminescent ELISA as described in Materials and Methods. Correlation of IgM titers to MDA-LDL with IgM titers to P1 (A) and P2 (C). Correlation of IgG titer to MDA-LDL with IgG titers to P1 (B) and P2 (D). Values are given as relative light units (RLU) per 100 ms and represent the mean of triplicate determinations. Data points represent measurements of titers obtained from each of the 18 subjects at four different times: 0, 30, 120, and 210 days. Correlations were calculated by analyzing all data from all subjects using nonparametric Spearman's rank correlation ( r = Spearman rank correlation coefficient). (E, F) Immunocompetition assays for specificity of IgG and IgM Abs to P1. Pooled human plasma was diluted 1:1,000 and incubated with or without increasing concentrations of BSA or MAA-BSA. Subsequently, samples were pelleted and binding of IgM (E) and IgG (F) Abs to coated P1 was determined in supernatants. Data are expressed as B/B 0 and represent the mean ± SD of triplicate determinations.
    Figure Legend Snippet: Mimotopes are recognized by human autoAbs and mimic MDA-LDL. (A–D) Correlation of mimotope and MDA-LDL-specific Ab titers in human plasma. Plasma of middle-aged healthy volunteers (n = 18) was obtained in a previous study ( 35 ) and Ab titers to P1, P2, and MDA-LDL were measured at 1:400 dilution by chemiluminescent ELISA as described in Materials and Methods. Correlation of IgM titers to MDA-LDL with IgM titers to P1 (A) and P2 (C). Correlation of IgG titer to MDA-LDL with IgG titers to P1 (B) and P2 (D). Values are given as relative light units (RLU) per 100 ms and represent the mean of triplicate determinations. Data points represent measurements of titers obtained from each of the 18 subjects at four different times: 0, 30, 120, and 210 days. Correlations were calculated by analyzing all data from all subjects using nonparametric Spearman's rank correlation ( r = Spearman rank correlation coefficient). (E, F) Immunocompetition assays for specificity of IgG and IgM Abs to P1. Pooled human plasma was diluted 1:1,000 and incubated with or without increasing concentrations of BSA or MAA-BSA. Subsequently, samples were pelleted and binding of IgM (E) and IgG (F) Abs to coated P1 was determined in supernatants. Data are expressed as B/B 0 and represent the mean ± SD of triplicate determinations.

    Techniques Used: Multiple Displacement Amplification, Chemiluminescent ELISA, Mass Spectrometry, Incubation, Binding Assay

    Plasma antibody binding to mimotopes over time in patients undergoing PCI. A–D: ELISA for binding of plasma IgG and IgM to P1 and P2 in patients that underwent PCI. Sequential plasma samples were obtained following PCI ( 32 ). Samples were diluted 1:400 and binding of IgM (A, B) and IgG (C, D) to coated P1 (10 μg/ml; A, C) and P2 (5 μg/ml; B, D) was determined by chemiluminescent ELISA. Shown are relative mean percent changes in Ab binding compared with values obtained at baseline (pre-PCI). * P
    Figure Legend Snippet: Plasma antibody binding to mimotopes over time in patients undergoing PCI. A–D: ELISA for binding of plasma IgG and IgM to P1 and P2 in patients that underwent PCI. Sequential plasma samples were obtained following PCI ( 32 ). Samples were diluted 1:400 and binding of IgM (A, B) and IgG (C, D) to coated P1 (10 μg/ml; A, C) and P2 (5 μg/ml; B, D) was determined by chemiluminescent ELISA. Shown are relative mean percent changes in Ab binding compared with values obtained at baseline (pre-PCI). * P

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Chemiluminescent ELISA

    8) Product Images from "Profiling of microorganism-binding serum antibody specificities in professional athletes"

    Article Title: Profiling of microorganism-binding serum antibody specificities in professional athletes

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0203665

    Graphic representation showing correlation coefficients obtained by correlating the string of reactivity of serum IgG ofdifferent individuals to different microorganism, obtained in ELISA, at steady state. A) Professional athletes; B) Control group. Red colour–Pearson product-moment correlation coefficient, r = 1, Blue– r = -1.
    Figure Legend Snippet: Graphic representation showing correlation coefficients obtained by correlating the string of reactivity of serum IgG ofdifferent individuals to different microorganism, obtained in ELISA, at steady state. A) Professional athletes; B) Control group. Red colour–Pearson product-moment correlation coefficient, r = 1, Blue– r = -1.

    Techniques Used: Enzyme-linked Immunosorbent Assay

    Graphic representation showing correlation coefficients obtained by correlating the string of reactivity of serumtotal IgG and total IgA with IgG subclasses, for different microorganisms in professional athletes. A) Escherichia coli ATCC25922; B) Candida albicans ATCC 10259; C) Lactobacillus plantarum WCFS1; D) Salmonella typhimurium 2865; E) Lactobacillusrhamnosus LGG, F) LPS from E . coli O55:B5.
    Figure Legend Snippet: Graphic representation showing correlation coefficients obtained by correlating the string of reactivity of serumtotal IgG and total IgA with IgG subclasses, for different microorganisms in professional athletes. A) Escherichia coli ATCC25922; B) Candida albicans ATCC 10259; C) Lactobacillus plantarum WCFS1; D) Salmonella typhimurium 2865; E) Lactobacillusrhamnosus LGG, F) LPS from E . coli O55:B5.

    Techniques Used:

    Analysis of reactivity of serial dilutions of serum IgG in A) professional athletes; B) control group, and of serum IgA in C) professional athletes; D) control group to different microorganisms. Red colour– E . coli ATCC25922; black - C . albicans ATCC 10259; turquoise– L . plantarum WCFS1; green– S . typhimurium 2865; blue— L . rhamnosus LGG, violet—LPS from E . coli 055:B5.
    Figure Legend Snippet: Analysis of reactivity of serial dilutions of serum IgG in A) professional athletes; B) control group, and of serum IgA in C) professional athletes; D) control group to different microorganisms. Red colour– E . coli ATCC25922; black - C . albicans ATCC 10259; turquoise– L . plantarum WCFS1; green– S . typhimurium 2865; blue— L . rhamnosus LGG, violet—LPS from E . coli 055:B5.

    Techniques Used:

    The strings of reactivities (apsorbance) of IgG and IgA to bacteria from different individuals were correlated, and the Pearson product-moment correlation coefficientswereplotted for individual bacteria. Red colour- Professional athletes PA; Green colour–Control.
    Figure Legend Snippet: The strings of reactivities (apsorbance) of IgG and IgA to bacteria from different individuals were correlated, and the Pearson product-moment correlation coefficientswereplotted for individual bacteria. Red colour- Professional athletes PA; Green colour–Control.

    Techniques Used:

    9) Product Images from "Rapid Generation of Human-Like Neutralizing Monoclonal Antibodies in Urgent Preparedness for Influenza Pandemics and Virulent Infectious Diseases"

    Article Title: Rapid Generation of Human-Like Neutralizing Monoclonal Antibodies in Urgent Preparedness for Influenza Pandemics and Virulent Infectious Diseases

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0066276

    Analysis of lungs from mice treated with mAb 4E6 and 1H10 after infection with H5N1 virus A/Vietnam/1194/04. (A) Viral copy number in the lungs at day 6 post-infection was determined using Q-PCR. The results are expressed as the mean ± SEM. (B) Virus titer in the lungs at day 6 post-infection was measured using a plaque formation assay in MDCK cells. The results are expressed as the mean ± SEM. (C) Histopathology in pulmonary tissue (40x magnification). 1) Mouse treated with mAb 4E6 at 24 hours post-infection. 2) Mouse treated with mAb 1H10 at 24 hours post-infection. 3) Mice injected with an irrelevant IgG at 24 hours post-infection were used as the control. HE: H E staining. IHC: immunohistochemical staining using a mAb against influenza nucleoprotein.
    Figure Legend Snippet: Analysis of lungs from mice treated with mAb 4E6 and 1H10 after infection with H5N1 virus A/Vietnam/1194/04. (A) Viral copy number in the lungs at day 6 post-infection was determined using Q-PCR. The results are expressed as the mean ± SEM. (B) Virus titer in the lungs at day 6 post-infection was measured using a plaque formation assay in MDCK cells. The results are expressed as the mean ± SEM. (C) Histopathology in pulmonary tissue (40x magnification). 1) Mouse treated with mAb 4E6 at 24 hours post-infection. 2) Mouse treated with mAb 1H10 at 24 hours post-infection. 3) Mice injected with an irrelevant IgG at 24 hours post-infection were used as the control. HE: H E staining. IHC: immunohistochemical staining using a mAb against influenza nucleoprotein.

    Techniques Used: Mouse Assay, Infection, Polymerase Chain Reaction, Plaque Formation Assay, Histopathology, Injection, Staining, Immunohistochemistry

    Therapeutic efficacy of mAb 4E6 and 1H10 in a mouse model of H5N1 virus A/Vietnam/1194/04 infection. (A) The Kaplan-Meier survival curve. BALB/c mice (n=7 per group in two separate experiments) were infected intranasally with 10 LD 50 of A/Vietnam/1194/04 and at 24, 48, or 72 hours, were treated with a single intraperitoneal injection of mAb 4E6 or 1H10 at 10 mg/kg body weight. The control group received IgG at 10 mg/kg body weight at 24 hours post-infection. (B) Percentage weight change in mice treated with mAb 4E6 or 1H10 at different time points post-infection. The mice (n=7) were infected via intranasal inoculation with 10 LD50 of A/Vietnam/1203/4, followed by a single intraperitoneal injection of mAbs at 24, 48, or 72 hours post-infection, and their weights were monitored for 14 days. The mice injected with an irrelevant IgG at 24 hours post-infection were used as the control group.
    Figure Legend Snippet: Therapeutic efficacy of mAb 4E6 and 1H10 in a mouse model of H5N1 virus A/Vietnam/1194/04 infection. (A) The Kaplan-Meier survival curve. BALB/c mice (n=7 per group in two separate experiments) were infected intranasally with 10 LD 50 of A/Vietnam/1194/04 and at 24, 48, or 72 hours, were treated with a single intraperitoneal injection of mAb 4E6 or 1H10 at 10 mg/kg body weight. The control group received IgG at 10 mg/kg body weight at 24 hours post-infection. (B) Percentage weight change in mice treated with mAb 4E6 or 1H10 at different time points post-infection. The mice (n=7) were infected via intranasal inoculation with 10 LD50 of A/Vietnam/1203/4, followed by a single intraperitoneal injection of mAbs at 24, 48, or 72 hours post-infection, and their weights were monitored for 14 days. The mice injected with an irrelevant IgG at 24 hours post-infection were used as the control group.

    Techniques Used: Infection, Mouse Assay, Injection

    10) Product Images from "Atheroprotective immunization with malondialdehyde-modified LDL is hapten specific and dependent on advanced MDA adducts: implications for development of an atheroprotective vaccine 1Atheroprotective immunization with malondialdehyde-modified LDL is hapten specific and dependent on advanced MDA adducts: implications for development of an atheroprotective vaccine 1 [S]"

    Article Title: Atheroprotective immunization with malondialdehyde-modified LDL is hapten specific and dependent on advanced MDA adducts: implications for development of an atheroprotective vaccine 1Atheroprotective immunization with malondialdehyde-modified LDL is hapten specific and dependent on advanced MDA adducts: implications for development of an atheroprotective vaccine 1 [S]

    Journal: Journal of Lipid Research

    doi: 10.1194/jlr.M053256

    MAA-MSA immunization induces and sustains an IgG1-dominant hapten-specific humoral immune response against MDHDC-lysine, demonstrating that MDHDC-lysine is an immunodominant epitope. A–F: Terminal antisera were pooled according to immunization groups, formally diluted, and tested for IgG1 (A, B), IgG2c (C), IgG2b (D), IgG3 (E), and IgM (F) binding to a semisynthetic MAA-modified biotinylated GDGDGK peptide [Bt-GDGDG-K(MAA)] (B–F) or the control peptide (A) by ELISA. The Bt-GDGDG-K(MAA) peptide was captured with plate-immobilized avidin to ensure reproducible epitope presentation and density. Data are curves of averaged triplicate determination in RLUs. G, H: A fixed 1:15,000 dilution of pooled terminal MAA-MSA antisera was preincubated in the presence or absence of synthetic GDGDG-K(MDHDC) (green diamonds; chemical structure shown in I) or GDGDG-K control (circles) peptide prior to direct plating of mixture on immobilized Bt-GDGDG-K(MAA) or MAA-BSA and detection of IgG1 binding by competition ELISA. Synthetic GDGDG-K(MDHDC) completely competes all IgG1 binding to immobilized Bt-GDGDG-K(MAA) illustrating that binding is specific to MDHDC-lysine. IgG1 binding to the more complex MAA-BSA antigen is less efficiently competed by GDGDG-K(MDHDC), showing that the MAA-MSA antisera contains Abs specific to MAA epitopes other than the MDHDC-lysine. Data are B/B 0, where each data point is the average of triplicate determination.
    Figure Legend Snippet: MAA-MSA immunization induces and sustains an IgG1-dominant hapten-specific humoral immune response against MDHDC-lysine, demonstrating that MDHDC-lysine is an immunodominant epitope. A–F: Terminal antisera were pooled according to immunization groups, formally diluted, and tested for IgG1 (A, B), IgG2c (C), IgG2b (D), IgG3 (E), and IgM (F) binding to a semisynthetic MAA-modified biotinylated GDGDGK peptide [Bt-GDGDG-K(MAA)] (B–F) or the control peptide (A) by ELISA. The Bt-GDGDG-K(MAA) peptide was captured with plate-immobilized avidin to ensure reproducible epitope presentation and density. Data are curves of averaged triplicate determination in RLUs. G, H: A fixed 1:15,000 dilution of pooled terminal MAA-MSA antisera was preincubated in the presence or absence of synthetic GDGDG-K(MDHDC) (green diamonds; chemical structure shown in I) or GDGDG-K control (circles) peptide prior to direct plating of mixture on immobilized Bt-GDGDG-K(MAA) or MAA-BSA and detection of IgG1 binding by competition ELISA. Synthetic GDGDG-K(MDHDC) completely competes all IgG1 binding to immobilized Bt-GDGDG-K(MAA) illustrating that binding is specific to MDHDC-lysine. IgG1 binding to the more complex MAA-BSA antigen is less efficiently competed by GDGDG-K(MDHDC), showing that the MAA-MSA antisera contains Abs specific to MAA epitopes other than the MDHDC-lysine. Data are B/B 0, where each data point is the average of triplicate determination.

    Techniques Used: Binding Assay, Modification, Enzyme-linked Immunosorbent Assay, Avidin-Biotin Assay

    11) Product Images from "Optimization of incubation conditions of Plasmodium falciparum antibody multiplex assays to measure IgG, IgG1–4, IgM and IgE using standard and customized reference pools for sero-epidemiological and vaccine studies"

    Article Title: Optimization of incubation conditions of Plasmodium falciparum antibody multiplex assays to measure IgG, IgG1–4, IgM and IgE using standard and customized reference pools for sero-epidemiological and vaccine studies

    Journal: Malaria Journal

    doi: 10.1186/s12936-018-2369-3

    IgG, IgG 1–4 and IgM fitted curves using the WHO-CSP pool to the 40-antigen multiplex panel incubating at 4 °C ON. Lines and dots represent predicted levels from 5PL, 4PL or exponential regression equations from 23 titration curves for IgG, IgG1, IgG3 and IgM; and 12 curves for IgG2 and IgG4. Titration curves contained 18 serial dilutions (1:2) starting at 1/50 of the WHO-CSP pool to a panel of 39 P. falciparum antigens plus HBsAg, α-Gal, BSA and GST
    Figure Legend Snippet: IgG, IgG 1–4 and IgM fitted curves using the WHO-CSP pool to the 40-antigen multiplex panel incubating at 4 °C ON. Lines and dots represent predicted levels from 5PL, 4PL or exponential regression equations from 23 titration curves for IgG, IgG1, IgG3 and IgM; and 12 curves for IgG2 and IgG4. Titration curves contained 18 serial dilutions (1:2) starting at 1/50 of the WHO-CSP pool to a panel of 39 P. falciparum antigens plus HBsAg, α-Gal, BSA and GST

    Techniques Used: Multiplex Assay, Titration

    RTS,S-specific responses measured in the WHO reference reagent, IgM pool and samples from RTS,S-vaccinated children. The 3 samples from RTS,S vaccinated children were of high, medium and low CSP IgG titres. a – g IgG, IgG 1–4 , IgM and IgE levels to RTS,S-specific antigens measured in the WHO reference reagent; IgG, IgG 1, IgG2 and IgG4 also measured in RTS,S-vaccinated children; h IgM levels to RTS,S-specific antigens measured in the IgM pool vs. RTS,S-vaccinated children. The plots represent the levels of antibodies measured in serial dilutions of the positive pools (1:3 starting at 1:50 for IgG, IgG 1–4 and IgM; and 1:2 starting at 1:10 for IgE), and the RTS,S vaccinees samples (1:10 starting at 1:500 for IgG, 1:100 for IgM, 1:50 for IgG 1–4 ; and 1:2 starting at 1:10 for IgE). Isolated dots represent the levels measured in the technical blanks
    Figure Legend Snippet: RTS,S-specific responses measured in the WHO reference reagent, IgM pool and samples from RTS,S-vaccinated children. The 3 samples from RTS,S vaccinated children were of high, medium and low CSP IgG titres. a – g IgG, IgG 1–4 , IgM and IgE levels to RTS,S-specific antigens measured in the WHO reference reagent; IgG, IgG 1, IgG2 and IgG4 also measured in RTS,S-vaccinated children; h IgM levels to RTS,S-specific antigens measured in the IgM pool vs. RTS,S-vaccinated children. The plots represent the levels of antibodies measured in serial dilutions of the positive pools (1:3 starting at 1:50 for IgG, IgG 1–4 and IgM; and 1:2 starting at 1:10 for IgE), and the RTS,S vaccinees samples (1:10 starting at 1:500 for IgG, 1:100 for IgM, 1:50 for IgG 1–4 ; and 1:2 starting at 1:10 for IgE). Isolated dots represent the levels measured in the technical blanks

    Techniques Used: Isolation

    12) Product Images from "Peptide mimotopes of malondialdehyde epitopes for clinical applications in cardiovascular disease [S]"

    Article Title: Peptide mimotopes of malondialdehyde epitopes for clinical applications in cardiovascular disease [S]

    Journal: Journal of Lipid Research

    doi: 10.1194/jlr.M025445

    MDA mimotope immunization induces MDA-specific Ab responses. (A, B) ELISA for the binding of plasma Abs of immunized mice to MDA-LDL, MAA-LDL, and P2. C57BL/6 mice were immunized with P2-BSA (n = 3) or BSA (n = 3) as described in Materials and Methods. Dilution curves of IgG1 and IgM binding to indicated antigens in pre- and postimmune plasma was determined by chemiluminescent ELISA. (A) Dilution curve of IgG1 binding. (B) Dilution curve of IgM binding. Values are given as relative light units (RLU) per 100 ms and represent the mean ± SEM of each group. (C) Immunohistochemical staining of human carotid endarterectomy specimens. Sections were stained with pooled preimmune (A) or postimmune plasma (B) of P2-BSA-immunized mice or with the MDA-LDL-specific mAb MDA2 (C). Positive staining is indicated by red color, and nuclei are counterstained with hematoxylin.
    Figure Legend Snippet: MDA mimotope immunization induces MDA-specific Ab responses. (A, B) ELISA for the binding of plasma Abs of immunized mice to MDA-LDL, MAA-LDL, and P2. C57BL/6 mice were immunized with P2-BSA (n = 3) or BSA (n = 3) as described in Materials and Methods. Dilution curves of IgG1 and IgM binding to indicated antigens in pre- and postimmune plasma was determined by chemiluminescent ELISA. (A) Dilution curve of IgG1 binding. (B) Dilution curve of IgM binding. Values are given as relative light units (RLU) per 100 ms and represent the mean ± SEM of each group. (C) Immunohistochemical staining of human carotid endarterectomy specimens. Sections were stained with pooled preimmune (A) or postimmune plasma (B) of P2-BSA-immunized mice or with the MDA-LDL-specific mAb MDA2 (C). Positive staining is indicated by red color, and nuclei are counterstained with hematoxylin.

    Techniques Used: Multiple Displacement Amplification, Enzyme-linked Immunosorbent Assay, Binding Assay, Mouse Assay, Chemiluminescent ELISA, Mass Spectrometry, Immunohistochemistry, Staining

    Mimotopes are recognized by human autoAbs and mimic MDA-LDL. (A–D) Correlation of mimotope and MDA-LDL-specific Ab titers in human plasma. Plasma of middle-aged healthy volunteers (n = 18) was obtained in a previous study ( 35 ) and Ab titers to P1, P2, and MDA-LDL were measured at 1:400 dilution by chemiluminescent ELISA as described in Materials and Methods. Correlation of IgM titers to MDA-LDL with IgM titers to P1 (A) and P2 (C). Correlation of IgG titer to MDA-LDL with IgG titers to P1 (B) and P2 (D). Values are given as relative light units (RLU) per 100 ms and represent the mean of triplicate determinations. Data points represent measurements of titers obtained from each of the 18 subjects at four different times: 0, 30, 120, and 210 days. Correlations were calculated by analyzing all data from all subjects using nonparametric Spearman's rank correlation ( r = Spearman rank correlation coefficient). (E, F) Immunocompetition assays for specificity of IgG and IgM Abs to P1. Pooled human plasma was diluted 1:1,000 and incubated with or without increasing concentrations of BSA or MAA-BSA. Subsequently, samples were pelleted and binding of IgM (E) and IgG (F) Abs to coated P1 was determined in supernatants. Data are expressed as B/B 0 and represent the mean ± SD of triplicate determinations.
    Figure Legend Snippet: Mimotopes are recognized by human autoAbs and mimic MDA-LDL. (A–D) Correlation of mimotope and MDA-LDL-specific Ab titers in human plasma. Plasma of middle-aged healthy volunteers (n = 18) was obtained in a previous study ( 35 ) and Ab titers to P1, P2, and MDA-LDL were measured at 1:400 dilution by chemiluminescent ELISA as described in Materials and Methods. Correlation of IgM titers to MDA-LDL with IgM titers to P1 (A) and P2 (C). Correlation of IgG titer to MDA-LDL with IgG titers to P1 (B) and P2 (D). Values are given as relative light units (RLU) per 100 ms and represent the mean of triplicate determinations. Data points represent measurements of titers obtained from each of the 18 subjects at four different times: 0, 30, 120, and 210 days. Correlations were calculated by analyzing all data from all subjects using nonparametric Spearman's rank correlation ( r = Spearman rank correlation coefficient). (E, F) Immunocompetition assays for specificity of IgG and IgM Abs to P1. Pooled human plasma was diluted 1:1,000 and incubated with or without increasing concentrations of BSA or MAA-BSA. Subsequently, samples were pelleted and binding of IgM (E) and IgG (F) Abs to coated P1 was determined in supernatants. Data are expressed as B/B 0 and represent the mean ± SD of triplicate determinations.

    Techniques Used: Multiple Displacement Amplification, Chemiluminescent ELISA, Mass Spectrometry, Incubation, Binding Assay

    Plasma antibody binding to mimotopes over time in patients undergoing PCI. A–D: ELISA for binding of plasma IgG and IgM to P1 and P2 in patients that underwent PCI. Sequential plasma samples were obtained following PCI ( 32 ). Samples were diluted 1:400 and binding of IgM (A, B) and IgG (C, D) to coated P1 (10 μg/ml; A, C) and P2 (5 μg/ml; B, D) was determined by chemiluminescent ELISA. Shown are relative mean percent changes in Ab binding compared with values obtained at baseline (pre-PCI). * P
    Figure Legend Snippet: Plasma antibody binding to mimotopes over time in patients undergoing PCI. A–D: ELISA for binding of plasma IgG and IgM to P1 and P2 in patients that underwent PCI. Sequential plasma samples were obtained following PCI ( 32 ). Samples were diluted 1:400 and binding of IgM (A, B) and IgG (C, D) to coated P1 (10 μg/ml; A, C) and P2 (5 μg/ml; B, D) was determined by chemiluminescent ELISA. Shown are relative mean percent changes in Ab binding compared with values obtained at baseline (pre-PCI). * P

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Chemiluminescent ELISA

    13) Product Images from "Pathogenicity Island 2 Mutants of Salmonella typhimurium Are Efficient Carriers for Heterologous Antigens and Enable Modulation of Immune Responses"

    Article Title: Pathogenicity Island 2 Mutants of Salmonella typhimurium Are Efficient Carriers for Heterologous Antigens and Enable Modulation of Immune Responses

    Journal: Infection and Immunity

    doi:

    Kinetics of LPS-specific (A) and β-Gal-specific (B) serum IgG (solid symbols) and IgM (open symbols) antibody responses in mice ( n = 5) after oral immunization with either MvP101(pAH97) (●), HH104(pAH97) (▴), SL7207(pAH97) (■), or carrier alone (⧫). Results are expressed as the reciprocal log 2 of the geometric mean endpoint titer (GMT). Standard deviations are indicated by vertical lines. Immunizations are indicated by arrows.
    Figure Legend Snippet: Kinetics of LPS-specific (A) and β-Gal-specific (B) serum IgG (solid symbols) and IgM (open symbols) antibody responses in mice ( n = 5) after oral immunization with either MvP101(pAH97) (●), HH104(pAH97) (▴), SL7207(pAH97) (■), or carrier alone (⧫). Results are expressed as the reciprocal log 2 of the geometric mean endpoint titer (GMT). Standard deviations are indicated by vertical lines. Immunizations are indicated by arrows.

    Techniques Used: Mouse Assay

    Related Articles

    Produced:

    Article Title: The in Vitro Antigenicity of Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2) B- and T-Epitopes Selected by HLA-DRB1 Binding Profile
    Article Snippet: .. A 1:1,000 dilution of monoclonal anti-human IgG1–biotin antibody produced in mouse (clone 8c/6-39), 1:15,000 monoclonal anti-human IgG2–biotin antibody produced in mouse (clone HP-6014), 1:40,000 monoclonal anti-human IgG3–biotin antibody produced in mouse (clone HP-6050) and 1:60,000 anti-human IgG4–biotin antibody, mouse monoclonal (clone HP-6025) (Sigma Aldrich) were used. .. ImmunoPure streptavidin, horseradish peroxidase conjugate (Thermo Scientific), at 1:5,000, dilution, was used as secondary antibody and TMB 2-Component Microwell peroxidase as substrate.

    Article Title: Profiling of microorganism-binding serum antibody specificities in professional athletes
    Article Snippet: .. For IgG subclass analysis, Biotin Mouse Anti-Human IgG1, IgG2, IgG4 (BD Biosciences, CA, USA), and Monoclonal Anti-Human IgG3-Biotin antibody produced in mouse (Sigma Aldrich) were used.All secondary antibodies were incubated for 1 hour at RT. .. After washing with PBS, streptavidin–horse radish peroxidase (Biolegend, San Diego, CA, USA) was added and incubated for 1 hour at RT.

    Incubation:

    Article Title: Profiling of microorganism-binding serum antibody specificities in professional athletes
    Article Snippet: .. For IgG subclass analysis, Biotin Mouse Anti-Human IgG1, IgG2, IgG4 (BD Biosciences, CA, USA), and Monoclonal Anti-Human IgG3-Biotin antibody produced in mouse (Sigma Aldrich) were used.All secondary antibodies were incubated for 1 hour at RT. .. After washing with PBS, streptavidin–horse radish peroxidase (Biolegend, San Diego, CA, USA) was added and incubated for 1 hour at RT.

    Article Title: Influence of camel milk on the hepatitis C virus burden of infected patients
    Article Snippet: .. After 1 h incubation at room temperature, the plates were washed five times, sequentially, with PBS and 50 µl biotin-labeled goat-antihuman IgG1 (1:1,000; cat. no. B6775), biotin-labeled goat-antihuman IgG2 (1:15,000; cat. no. B3398), biotin-labeled goat-antihuman IgG3 (1:4,000; cat. no. B3523), or biotin-labeled goat-antihuman IgG4 (1:15,000; cat. no. B3648; Sigma-Aldrich; Merck Millipore, Darmstadt, Germany) were added. .. The plates were washed five times to remove unbound antibodies and 50 µl streptavidin-alkaline phosphatase-conjugate (Sigma-Aldrich; Merck Millipore) diluted 1:4,000 with 2% BSA-PBS was subsequently added.

    Article Title: Distinct Autoimmune Anti-α-Synuclein Antibody Patterns in Multiple System Atrophy and Parkinson’s Disease
    Article Snippet: .. For IgG4 assays, plasma samples were diluted 1:50. (4) After a subsequent washing step, the plates were incubated at RT for 2 h with 50 μl of biotinylated secondary antibodies at the following concentrations: goat anti-human total IgG 1:30,000 (Sigma-Aldrich, cat# SAB3701279, RRID:AB_2783655 ), goat anti-human IgM (1:5,000; Sigma-Aldrich, cat# B1265, RRID:AB_258514 ), mouse anti-human IgG1 (1:1,000; Thermo Fisher Scientific, Cat# MH1515, RRID:AB_2539710 ), mouse anti-human IgG2 (1:5,000: Sigma-Aldrich, cat# B3398, RRID:AB_258546 ), mouse anti-human IgG3 (1:500; Sigma-Aldrich, cat# B3523, RRID:AB_258549 ), and mouse anti-human IgG4 (1:200; Sigma-Aldrich, cat# B3648, RRID:AB_258555 ). .. After washing, 50 μl portions of streptavidin-peroxidase (1:10,000; Sigma-Aldrich, cat# 5512) were added to each well and incubated for 30 min at RT. (5) The plates were washed for the last time before the enzymatic reaction was developed by adding 50 μl of tetramethylbenzidine (TMB) Liquid Peroxidase Substrate (Sigma-Aldrich, cat# T8665) and incubated in dark for 30 min at RT. (6) The enzymatic reaction was terminated by addition of 0.5 N Sulfuric Acid (Merck, cat# 109073), and (7) the optical density (OD) was measured on a MultiscanTM FC Microplate reader (Fisher ScientificTM ) at 450/620 nm.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Millipore anti rabbit igg gamma chain specific biotin antibody mouse monoclonal
    Anti Rabbit Igg Gamma Chain Specific Biotin Antibody Mouse Monoclonal, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rabbit igg gamma chain specific biotin antibody mouse monoclonal/product/Millipore
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti rabbit igg gamma chain specific biotin antibody mouse monoclonal - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    Image Search Results