monoclonal anti adipophilin antibody  (Santa Cruz Biotechnology)

 
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    Name:
    ADRP Antibody
    Description:
    Anti ADRP Antibody B 6 is a mouse monoclonal IgG1 kappa light chain ADRP antibody provided at 200 µg ml raised against amino acids 321 400 mapping near the C terminus of ADRP of human origin ADRP Antibody B 6 is recommended for detection of ADRP of human origin by WB IP IF IHC P and ELISA Anti ADRP Antibody B 6 is available conjugated to agarose for IP HRP for WB IHC P and ELISA and to either phycoerythrin or FITC for IF IHC P and FCM also available conjugated to Alexa Fluor 488 Alexa Fluor 546 Alexa Fluor 594 or Alexa Fluor 647 for WB RGB IF IHC P and FCM and for use with RGB fluorescent imaging systems such as iBright FL1000 FluorChem Typhoon Azure and other comparable systems also available conjugated to Alexa Fluor 680 or Alexa Fluor 790 for WB NIR IF and FCM for use with Near Infrared NIR detection systems such as LI COR Odyssey iBright FL1000 FluorChem Typhoon Azure and other comparable systems Contact our Technical Service Department or your local Distributor for more information on how to receive a FREE 10 µg sample of ADRP B 6 sc 377429
    Catalog Number:
    SC-377429
    Price:
    None
    Category:
    Antibodies Primary Antibodies and ImmunoCruz Conjugates Signaling Intermediates ADRP Antibodies ADRP Antibody B 6
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    Structured Review

    Santa Cruz Biotechnology monoclonal anti adipophilin antibody
    Microscopic examination of the mass in a male wild nutria. (A) The mass is composed of reserved basal cells and secretory cells containing oily and waxy matter, which constitute a type of sebaceous gland. The gland has a long excretory duct with a wide lumen lined by hyperplastic squamous epithelium. (B, C) The tumor cells have a central round nucleus with one or (rarely) 2 large nucleoli. The cytoplasm contains small to large vacuoles. (D) <t>Adipophilin</t> is expressed in intracytoplasmic lipid vacuoles in sebaceous cells. Hematoxylin and eosin (A-C); immunohistochemistry of adipophilin (D) (all scale bar = 100 µm).
    Anti ADRP Antibody B 6 is a mouse monoclonal IgG1 kappa light chain ADRP antibody provided at 200 µg ml raised against amino acids 321 400 mapping near the C terminus of ADRP of human origin ADRP Antibody B 6 is recommended for detection of ADRP of human origin by WB IP IF IHC P and ELISA Anti ADRP Antibody B 6 is available conjugated to agarose for IP HRP for WB IHC P and ELISA and to either phycoerythrin or FITC for IF IHC P and FCM also available conjugated to Alexa Fluor 488 Alexa Fluor 546 Alexa Fluor 594 or Alexa Fluor 647 for WB RGB IF IHC P and FCM and for use with RGB fluorescent imaging systems such as iBright FL1000 FluorChem Typhoon Azure and other comparable systems also available conjugated to Alexa Fluor 680 or Alexa Fluor 790 for WB NIR IF and FCM for use with Near Infrared NIR detection systems such as LI COR Odyssey iBright FL1000 FluorChem Typhoon Azure and other comparable systems Contact our Technical Service Department or your local Distributor for more information on how to receive a FREE 10 µg sample of ADRP B 6 sc 377429
    https://www.bioz.com/result/monoclonal anti adipophilin antibody/product/Santa Cruz Biotechnology
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal anti adipophilin antibody - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Preputial gland adenoma in a wild nutria (Myocastor coypus): a case report"

    Article Title: Preputial gland adenoma in a wild nutria (Myocastor coypus): a case report

    Journal: Journal of Veterinary Science

    doi: 10.4142/jvs.2020.21.e1

    Microscopic examination of the mass in a male wild nutria. (A) The mass is composed of reserved basal cells and secretory cells containing oily and waxy matter, which constitute a type of sebaceous gland. The gland has a long excretory duct with a wide lumen lined by hyperplastic squamous epithelium. (B, C) The tumor cells have a central round nucleus with one or (rarely) 2 large nucleoli. The cytoplasm contains small to large vacuoles. (D) Adipophilin is expressed in intracytoplasmic lipid vacuoles in sebaceous cells. Hematoxylin and eosin (A-C); immunohistochemistry of adipophilin (D) (all scale bar = 100 µm).
    Figure Legend Snippet: Microscopic examination of the mass in a male wild nutria. (A) The mass is composed of reserved basal cells and secretory cells containing oily and waxy matter, which constitute a type of sebaceous gland. The gland has a long excretory duct with a wide lumen lined by hyperplastic squamous epithelium. (B, C) The tumor cells have a central round nucleus with one or (rarely) 2 large nucleoli. The cytoplasm contains small to large vacuoles. (D) Adipophilin is expressed in intracytoplasmic lipid vacuoles in sebaceous cells. Hematoxylin and eosin (A-C); immunohistochemistry of adipophilin (D) (all scale bar = 100 µm).

    Techniques Used: Immunohistochemistry

    Related Articles

    Immunohistochemistry:

    Article Title: Preputial gland adenoma in a wild nutria (Myocastor coypus): a case report
    Article Snippet: .. For immunohistochemistry (IHC) analysis, monoclonal anti-adipophilin antibody (sc-377429; Santa Cruz Biotechnology, USA) was used to detect sebaceous cells. ..

    Fluorescence:

    Article Title: Synaptogyrin-2 Promotes Replication of a Novel Tick-borne Bunyavirus through Interacting with Viral Nonstructural Protein NSs *
    Article Snippet: .. Antibodies for anti-SYNGR2 antibody, anti-ADRP antibody, anti-perilipin A, and secondary antibodies with fluorescence-labeled Alex Fluor 647 donkey anti-mouse and Alex Fluor 594 goat anti-rabbit IgG were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). ..

    Staining:

    Article Title: Glycation by glyoxal leads to profound changes in the behavior of dermal fibroblasts
    Article Snippet: .. After 24 hours, 0.6 mM glyoxal, 1 mM AICAR (5-aminoimidazole-4-carboxamide ribonucleotide) (Sigma #A9978), or both, were added for a further 24 hours and the cells were then fixed with 2% formaldehyde for 20 min. BODIPY 493/503 (Thermo Fisher Scientific, #D3922) and anti-perilipin-2 staining (Santa Cruz Biotechnology, #sc-377429) were performed according to the protocol of Listenberger et al. ..

    Blocking Assay:

    Article Title: Protective Effects of Curcumin on Renal Oxidative Stress and Lipid Metabolism in a Rat Model of Type 2 Diabetic Nephropathy
    Article Snippet: .. The membrane was hybridized with anti-p-AMPK, anti-AMPK, and anti-p-ACC antibodies (Cell Signaling Technology Inc., Danvers, MA, USA), or anti-β-actin, anti-Nrf2, anti-Keap1, anti-HO-1, anti-vascular endothelial growth factor, anti-tumor growth factor-β1, anti-SREBP1, anti-SREBP2, and anti-adipose differentiation related protein (ADRP) antibodies (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) in a blocking buffer overnight at 4℃. ..

    other:

    Article Title: Inhibition of the Lipid Droplet–Peroxisome Proliferator-Activated Receptor α Axis Suppresses Cancer Stem Cell Properties
    Article Snippet: ADRP (PRIN2; sc-377429) and adipose triglyceride lipase (ATGL) (sc-365278) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Similar Products

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  • 94
    Santa Cruz Biotechnology monoclonal anti adipophilin antibody
    Microscopic examination of the mass in a male wild nutria. (A) The mass is composed of reserved basal cells and secretory cells containing oily and waxy matter, which constitute a type of sebaceous gland. The gland has a long excretory duct with a wide lumen lined by hyperplastic squamous epithelium. (B, C) The tumor cells have a central round nucleus with one or (rarely) 2 large nucleoli. The cytoplasm contains small to large vacuoles. (D) <t>Adipophilin</t> is expressed in intracytoplasmic lipid vacuoles in sebaceous cells. Hematoxylin and eosin (A-C); immunohistochemistry of adipophilin (D) (all scale bar = 100 µm).
    Monoclonal Anti Adipophilin Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal anti adipophilin antibody/product/Santa Cruz Biotechnology
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal anti adipophilin antibody - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    93
    Santa Cruz Biotechnology monoclonal mouse anti hmgcs2
    CLUH granules are translationally active or dormant depending on the mRNA Confocal images of primary hepatocytes after ribopuromycylation experiment combined with mRNA in situ hybridization for (A) Hadha , (B) Pcx, and (C) <t>Hmgcs2</t> . Scale bar, 4 μm. Fluorescence profile of 100‐pixel line from the merged channel shown in (A–C). The cells from which the enlarged areas (400 μm 2 ) have been magnified are shown in Appendix Fig S2 for each individual channel. Cells analyzed were isolated from 6 different mice with similar results.
    Monoclonal Mouse Anti Hmgcs2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal mouse anti hmgcs2/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal mouse anti hmgcs2 - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Microscopic examination of the mass in a male wild nutria. (A) The mass is composed of reserved basal cells and secretory cells containing oily and waxy matter, which constitute a type of sebaceous gland. The gland has a long excretory duct with a wide lumen lined by hyperplastic squamous epithelium. (B, C) The tumor cells have a central round nucleus with one or (rarely) 2 large nucleoli. The cytoplasm contains small to large vacuoles. (D) Adipophilin is expressed in intracytoplasmic lipid vacuoles in sebaceous cells. Hematoxylin and eosin (A-C); immunohistochemistry of adipophilin (D) (all scale bar = 100 µm).

    Journal: Journal of Veterinary Science

    Article Title: Preputial gland adenoma in a wild nutria (Myocastor coypus): a case report

    doi: 10.4142/jvs.2020.21.e1

    Figure Lengend Snippet: Microscopic examination of the mass in a male wild nutria. (A) The mass is composed of reserved basal cells and secretory cells containing oily and waxy matter, which constitute a type of sebaceous gland. The gland has a long excretory duct with a wide lumen lined by hyperplastic squamous epithelium. (B, C) The tumor cells have a central round nucleus with one or (rarely) 2 large nucleoli. The cytoplasm contains small to large vacuoles. (D) Adipophilin is expressed in intracytoplasmic lipid vacuoles in sebaceous cells. Hematoxylin and eosin (A-C); immunohistochemistry of adipophilin (D) (all scale bar = 100 µm).

    Article Snippet: For immunohistochemistry (IHC) analysis, monoclonal anti-adipophilin antibody (sc-377429; Santa Cruz Biotechnology, USA) was used to detect sebaceous cells.

    Techniques: Immunohistochemistry

    CLUH granules are translationally active or dormant depending on the mRNA Confocal images of primary hepatocytes after ribopuromycylation experiment combined with mRNA in situ hybridization for (A) Hadha , (B) Pcx, and (C) Hmgcs2 . Scale bar, 4 μm. Fluorescence profile of 100‐pixel line from the merged channel shown in (A–C). The cells from which the enlarged areas (400 μm 2 ) have been magnified are shown in Appendix Fig S2 for each individual channel. Cells analyzed were isolated from 6 different mice with similar results.

    Journal: The EMBO Journal

    Article Title: CLUH granules coordinate translation of mitochondrial proteins with mTORC1 signaling and mitophagy

    doi: 10.15252/embj.2019102731

    Figure Lengend Snippet: CLUH granules are translationally active or dormant depending on the mRNA Confocal images of primary hepatocytes after ribopuromycylation experiment combined with mRNA in situ hybridization for (A) Hadha , (B) Pcx, and (C) Hmgcs2 . Scale bar, 4 μm. Fluorescence profile of 100‐pixel line from the merged channel shown in (A–C). The cells from which the enlarged areas (400 μm 2 ) have been magnified are shown in Appendix Fig S2 for each individual channel. Cells analyzed were isolated from 6 different mice with similar results.

    Article Snippet: The following antibodies were used for Western blot: polyclonal rabbit anti‐4E‐BP1‐pT37/46 (#2855, clone 236B4), polyclonal rabbit anti‐4E‐BP1(#9452), monoclonal rabbit anti‐VDAC1 (#4661, clone D73D12), and polyclonal rabbit anti‐RPS6‐pS235/236 (#2211) from Cell Signaling; monoclonal mouse anti‐GAPDH (#MAB374, clone 6C5) and monoclonal mouse anti‐pan‐actin (#MAB1501R, clone C4) from EMD Millipore; polyclonal rabbit anti‐CLUH (for detection of human CLUH, #NB100‐93306), polyclonal rabbit anti‐RPS6 (#NB100‐1595), and polyclonal rabbit anti‐LC3 (#NB100‐2220) from Novus Biologicals; polyclonal rabbit anti‐CLUH (for detection of murine CLUH, # ARP70642_P050) from Aviva; monoclonal mouse anti‐p62 (#H00008878‐M01, clone 2C11) from Abnova; monoclonal mouse anti‐ubiquitin (#sc‐8017, clone P4D1) and monoclonal mouse anti‐HMGCS2 (#sc‐376092, clone G‐11) from Santa Cruz Biotechnology; and polyclonal rabbit anti‐HADHA (#ab54477), monoclonal rabbit anti‐PCX (#ab128952, clone EPR7365), monoclonal rabbit anti‐G3BP1 (#ab181150, clone EPR13986(B)), polyclonal rabbit anti‐G3BP2 (#ab86135), and polyclonal rabbit anti‐LAMP1 (#ab24170) from Abcam.

    Techniques: In Situ Hybridization, Fluorescence, Isolation, Mouse Assay

    Model of CLUH function in the liver CLUH forms granules containing target mRNAs and regulates their translation. In addition, CLUH granules recruit G3BP1, G3BP2, and mTOR, thereby enhancing mitophagy and inhibiting mitochondrial anabolic pathways. Together, these roles of CLUH are crucial in the liver to survive starvation. In the absence of CLUH, CLUH granules do not form resulting in reduced translation of Hmgcs2 , Hadha, and Pcx mRNAs, in failure to suppress mTORC1‐dependent anabolic pathways, and in a block in mitophagy.

    Journal: The EMBO Journal

    Article Title: CLUH granules coordinate translation of mitochondrial proteins with mTORC1 signaling and mitophagy

    doi: 10.15252/embj.2019102731

    Figure Lengend Snippet: Model of CLUH function in the liver CLUH forms granules containing target mRNAs and regulates their translation. In addition, CLUH granules recruit G3BP1, G3BP2, and mTOR, thereby enhancing mitophagy and inhibiting mitochondrial anabolic pathways. Together, these roles of CLUH are crucial in the liver to survive starvation. In the absence of CLUH, CLUH granules do not form resulting in reduced translation of Hmgcs2 , Hadha, and Pcx mRNAs, in failure to suppress mTORC1‐dependent anabolic pathways, and in a block in mitophagy.

    Article Snippet: The following antibodies were used for Western blot: polyclonal rabbit anti‐4E‐BP1‐pT37/46 (#2855, clone 236B4), polyclonal rabbit anti‐4E‐BP1(#9452), monoclonal rabbit anti‐VDAC1 (#4661, clone D73D12), and polyclonal rabbit anti‐RPS6‐pS235/236 (#2211) from Cell Signaling; monoclonal mouse anti‐GAPDH (#MAB374, clone 6C5) and monoclonal mouse anti‐pan‐actin (#MAB1501R, clone C4) from EMD Millipore; polyclonal rabbit anti‐CLUH (for detection of human CLUH, #NB100‐93306), polyclonal rabbit anti‐RPS6 (#NB100‐1595), and polyclonal rabbit anti‐LC3 (#NB100‐2220) from Novus Biologicals; polyclonal rabbit anti‐CLUH (for detection of murine CLUH, # ARP70642_P050) from Aviva; monoclonal mouse anti‐p62 (#H00008878‐M01, clone 2C11) from Abnova; monoclonal mouse anti‐ubiquitin (#sc‐8017, clone P4D1) and monoclonal mouse anti‐HMGCS2 (#sc‐376092, clone G‐11) from Santa Cruz Biotechnology; and polyclonal rabbit anti‐HADHA (#ab54477), monoclonal rabbit anti‐PCX (#ab128952, clone EPR7365), monoclonal rabbit anti‐G3BP1 (#ab181150, clone EPR13986(B)), polyclonal rabbit anti‐G3BP2 (#ab86135), and polyclonal rabbit anti‐LAMP1 (#ab24170) from Abcam.

    Techniques: Blocking Assay