Anti Mhc A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Twitchin kinase interacts with MAPKAP kinase 2 in Caenorhabditis elegans striated muscle"
Article Title: Twitchin kinase interacts with MAPKAP kinase 2 in Caenorhabditis elegans striated muscle
Journal: Molecular Biology of the Cell
Figure Legend Snippet: Phenotype of mak-1 mutants. (A) A mak-1 mutant has slightly reduced locomotion. Results of swimming assays on adult nematodes of the indicated genotypes. Data are means and SEs with n = 20. Although mak-1 ( tm3455 ) animals show almost the same motility as wild-type animals, mak-1 ( ok2987 ) animals show slightly reduced motility that is statistically significant. The unc-22 –null allele, ct37 , and the unc-22 canonical allele, e66 , are much slower than wild type. In contrast, unc-22 ( e105 ) shows normal or even increased locomotion in this assay. (B) mak-1 ( ok2987 ) shows normal sarcomeric structure. Images show part of a body wall muscle cell from either wild type or mak-1 ( ok2987 ) immunostained with the indicated antibodies. UNC-52 (perlecan), UNC-112, and UNC-95 all localize to M-lines and dense bodies; UNC-52 in the extracellular matrix; and UNC-112 and UNC-95 in the muscle cytoplasm close to the cell membrane. ATN-1 (α-actinin) localizes to the main, deeper portion of the dense bodies. UNC-89 (obscurin) is located at M-lines full depth, from muscle cell membrane, up through the deepest part of the myofilament lattice. Twitchin localizes to the outer portions of A-bands, and MHC A localizes to the middle of A-bands. For all these proteins, localization is no different in mak-1 ( ok2987 ) muscle than in wild-type muscle. Scale bar, 10 μm.
Techniques Used: Mutagenesis