anti mhcii  (Thermo Fisher)


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    Structured Review

    Thermo Fisher anti mhcii
    Disturbance of membrane traffic within the endocytic system of SPPL2a −/− B cells. (A and B) Visualization of CD74 in isolated splenic SPPL2a +/+ and SPPL2a −/− B cells by indirect immunofluorescence using an antibody against an N-terminal epitope detecting the NTF and the full-length protein. EEA1 (A) and LAMP1 (B) served as markers of early endosomes and lysosomes/late endosomes, respectively. Bars, 2 µm. (C) Transmission electron microscopy of splenic IgM + B cells from wild-type or SPPL2a −/− mice. Bars, 1 µm. (D) Vacuoles in SPPL2a −/− B cells exhibited various contents of low-electron density. Occasionally, multivesicular bodies (mvb) were observed (arrow). Bar, 500 nm. (E) Presence of CD74 NTF, LAMP1, and <t>MHCII</t> in vacuoles of IgM + B cells from SPPL2a −/− mice was assessed by immunogold labeling. Bars, 200 nm (CD74 and MHCII single labeling) or 100 nm (CD74 + LAMP1 double labeling). (F and G) Surface and total MHCII levels in transitional stage T1 B cells (B220 + CD21 low CD24 high ) of SPPL2a-deficient or wild-type mice. Splenocytes were stained for B220, CD21, and CD24, allowing for identification of B cell subsets. Subsequently, cells were incubated with <t>anti-MHCII</t> with or without previous permeabilization and analyzed by flow cytometry. Surface and total MHCII levels are shown as histograms representative of three independent experiments or as mean of median fluorescence intensity (MFI) from three mice per genotype (G). ***, P
    Anti Mhcii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 2 article reviews
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    anti mhcii - by Bioz Stars, 2022-09
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    Images

    1) Product Images from "The intramembrane protease SPPL2a promotes B cell development and controls endosomal traffic by cleavage of the invariant chain"

    Article Title: The intramembrane protease SPPL2a promotes B cell development and controls endosomal traffic by cleavage of the invariant chain

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20121069

    Disturbance of membrane traffic within the endocytic system of SPPL2a −/− B cells. (A and B) Visualization of CD74 in isolated splenic SPPL2a +/+ and SPPL2a −/− B cells by indirect immunofluorescence using an antibody against an N-terminal epitope detecting the NTF and the full-length protein. EEA1 (A) and LAMP1 (B) served as markers of early endosomes and lysosomes/late endosomes, respectively. Bars, 2 µm. (C) Transmission electron microscopy of splenic IgM + B cells from wild-type or SPPL2a −/− mice. Bars, 1 µm. (D) Vacuoles in SPPL2a −/− B cells exhibited various contents of low-electron density. Occasionally, multivesicular bodies (mvb) were observed (arrow). Bar, 500 nm. (E) Presence of CD74 NTF, LAMP1, and MHCII in vacuoles of IgM + B cells from SPPL2a −/− mice was assessed by immunogold labeling. Bars, 200 nm (CD74 and MHCII single labeling) or 100 nm (CD74 + LAMP1 double labeling). (F and G) Surface and total MHCII levels in transitional stage T1 B cells (B220 + CD21 low CD24 high ) of SPPL2a-deficient or wild-type mice. Splenocytes were stained for B220, CD21, and CD24, allowing for identification of B cell subsets. Subsequently, cells were incubated with anti-MHCII with or without previous permeabilization and analyzed by flow cytometry. Surface and total MHCII levels are shown as histograms representative of three independent experiments or as mean of median fluorescence intensity (MFI) from three mice per genotype (G). ***, P
    Figure Legend Snippet: Disturbance of membrane traffic within the endocytic system of SPPL2a −/− B cells. (A and B) Visualization of CD74 in isolated splenic SPPL2a +/+ and SPPL2a −/− B cells by indirect immunofluorescence using an antibody against an N-terminal epitope detecting the NTF and the full-length protein. EEA1 (A) and LAMP1 (B) served as markers of early endosomes and lysosomes/late endosomes, respectively. Bars, 2 µm. (C) Transmission electron microscopy of splenic IgM + B cells from wild-type or SPPL2a −/− mice. Bars, 1 µm. (D) Vacuoles in SPPL2a −/− B cells exhibited various contents of low-electron density. Occasionally, multivesicular bodies (mvb) were observed (arrow). Bar, 500 nm. (E) Presence of CD74 NTF, LAMP1, and MHCII in vacuoles of IgM + B cells from SPPL2a −/− mice was assessed by immunogold labeling. Bars, 200 nm (CD74 and MHCII single labeling) or 100 nm (CD74 + LAMP1 double labeling). (F and G) Surface and total MHCII levels in transitional stage T1 B cells (B220 + CD21 low CD24 high ) of SPPL2a-deficient or wild-type mice. Splenocytes were stained for B220, CD21, and CD24, allowing for identification of B cell subsets. Subsequently, cells were incubated with anti-MHCII with or without previous permeabilization and analyzed by flow cytometry. Surface and total MHCII levels are shown as histograms representative of three independent experiments or as mean of median fluorescence intensity (MFI) from three mice per genotype (G). ***, P

    Techniques Used: Isolation, Immunofluorescence, Transmission Assay, Electron Microscopy, Mouse Assay, Labeling, Staining, Incubation, Flow Cytometry, Fluorescence

    The intramembrane protease SPPL2a cleaves CD74 NTF. (A) Scheme of proteolytic degradation of CD74 in MHCII compartments, where the luminal domain is removed in a stepwise fashion by endosomal proteases and finally released from the MHCII dimer by cathepsin S. A small fragment (CLIP) persists inside the peptide-binding groove of MHCII, which is subsequently replaced with an antigenic peptide before the MHCII–peptide complex is transported to the plasma membrane. The remaining transmembrane NTF (82 aa) of CD74 is then proteolyzed by SPPL2a. The catalytically critical YD and GxGD motifs of SPPL2a are indicated by colored asterisks. (B) HEK293 cells stably expressing the p31 isoform of CD74 (HA-CD74p31-V5) were treated with 10 µM (Z-LL) 2 -ketone, 1 µM inhibitor X, 100 µM leupeptin or 25 mM NH 4 Cl for 5 h. The CD74 NTF is indicated by the open arrowheads. Full-length CD74 (closed arrowheads) and CD74 NTF were detected with anti-HA recognizing the epitope tag fused to the N terminus of the protein. (C) Transient knockdown of SPPL2a in HEK293 cells stably expressing HA-tagged CD74 (HA-CD74p31-V5). SPPL2a and the lysosomal membrane protein LAMP2 as control were analyzed in carbonate-washed membranes from the same batch of cells for enhancing SPPL2a detectability. (D) SPPL2a or the inactive D416A mutant were transiently co-expressed with CD74, followed by detection with anti-CD74 (In-1). (E) Using an antibody against an N-terminal epitope of CD74, endogenous CD74 was analyzed in splenic IgM + B cells isolated from SPPL2a −/− and control mice. (B–E) Electrophoretic separation before detection of CD74 was performed by standard Tris-Glycine SDS-PAGE (D) or using a Tris-Tricine buffer system (B, C, and E) with improved resolution in the low-molecular weight range. Equal protein loading was confirmed as indicated. Data are representative of three independent experiments.
    Figure Legend Snippet: The intramembrane protease SPPL2a cleaves CD74 NTF. (A) Scheme of proteolytic degradation of CD74 in MHCII compartments, where the luminal domain is removed in a stepwise fashion by endosomal proteases and finally released from the MHCII dimer by cathepsin S. A small fragment (CLIP) persists inside the peptide-binding groove of MHCII, which is subsequently replaced with an antigenic peptide before the MHCII–peptide complex is transported to the plasma membrane. The remaining transmembrane NTF (82 aa) of CD74 is then proteolyzed by SPPL2a. The catalytically critical YD and GxGD motifs of SPPL2a are indicated by colored asterisks. (B) HEK293 cells stably expressing the p31 isoform of CD74 (HA-CD74p31-V5) were treated with 10 µM (Z-LL) 2 -ketone, 1 µM inhibitor X, 100 µM leupeptin or 25 mM NH 4 Cl for 5 h. The CD74 NTF is indicated by the open arrowheads. Full-length CD74 (closed arrowheads) and CD74 NTF were detected with anti-HA recognizing the epitope tag fused to the N terminus of the protein. (C) Transient knockdown of SPPL2a in HEK293 cells stably expressing HA-tagged CD74 (HA-CD74p31-V5). SPPL2a and the lysosomal membrane protein LAMP2 as control were analyzed in carbonate-washed membranes from the same batch of cells for enhancing SPPL2a detectability. (D) SPPL2a or the inactive D416A mutant were transiently co-expressed with CD74, followed by detection with anti-CD74 (In-1). (E) Using an antibody against an N-terminal epitope of CD74, endogenous CD74 was analyzed in splenic IgM + B cells isolated from SPPL2a −/− and control mice. (B–E) Electrophoretic separation before detection of CD74 was performed by standard Tris-Glycine SDS-PAGE (D) or using a Tris-Tricine buffer system (B, C, and E) with improved resolution in the low-molecular weight range. Equal protein loading was confirmed as indicated. Data are representative of three independent experiments.

    Techniques Used: Cross-linking Immunoprecipitation, Binding Assay, Stable Transfection, Expressing, Mutagenesis, Isolation, Mouse Assay, SDS Page, Molecular Weight

    2) Product Images from "A Novel Cellular Pathway of Antigen Presentation and CD4 T Cell Activation in vivo"

    Article Title: A Novel Cellular Pathway of Antigen Presentation and CD4 T Cell Activation in vivo

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2018.02684

    Recruited monocytes present antigen and may be observed interacting with T cells in the lymph node at 24 h post-immunization. (A) Representative plots showing flow cytometry gating strategy for the identification of CD11b+CD64+ monocytes, for CD11b+CD169+ SCS macrophages and CD11b- or CD11b+ CD11c+MHCII+ dendritic cells. Graphs showing mean (±SEM) YAe MFI on CD11b- (B) and CD11b+ (C) dendritic cells at 4, 24, and 48 h; mean (±SEM) YAe MFI SCS macrophages (D) at 4 h; mean (±SEM) YAe MFI on monocytes (E) at 4, 24, and 48 h. Groups contained 3 animals and data is representative of three independent experiments. (F) LysM-EGFP or CD11cYFP mice were immunized in the footpad (OVA or BSA/ISCOMATRIX or OVA/LPS respectively) ~20 h later the draining popliteal lymph node was surgically exposed. OTII DSRed T cells and monocytes (GFP+) or DCs (YFP+) were imaged by live real time multiphoton microscopy. Interaction between T cells and monocytes (and T cells and DCs was identified by colocalization of the DSRed signal with the GFP or YFP signals and tracked for up to 20 min, 48 such interactions were tracked in the OVA/ISCOMATRIX treated mice. IMX = ISCOMATRIX™ adjuvant, * P
    Figure Legend Snippet: Recruited monocytes present antigen and may be observed interacting with T cells in the lymph node at 24 h post-immunization. (A) Representative plots showing flow cytometry gating strategy for the identification of CD11b+CD64+ monocytes, for CD11b+CD169+ SCS macrophages and CD11b- or CD11b+ CD11c+MHCII+ dendritic cells. Graphs showing mean (±SEM) YAe MFI on CD11b- (B) and CD11b+ (C) dendritic cells at 4, 24, and 48 h; mean (±SEM) YAe MFI SCS macrophages (D) at 4 h; mean (±SEM) YAe MFI on monocytes (E) at 4, 24, and 48 h. Groups contained 3 animals and data is representative of three independent experiments. (F) LysM-EGFP or CD11cYFP mice were immunized in the footpad (OVA or BSA/ISCOMATRIX or OVA/LPS respectively) ~20 h later the draining popliteal lymph node was surgically exposed. OTII DSRed T cells and monocytes (GFP+) or DCs (YFP+) were imaged by live real time multiphoton microscopy. Interaction between T cells and monocytes (and T cells and DCs was identified by colocalization of the DSRed signal with the GFP or YFP signals and tracked for up to 20 min, 48 such interactions were tracked in the OVA/ISCOMATRIX treated mice. IMX = ISCOMATRIX™ adjuvant, * P

    Techniques Used: Flow Cytometry, Cytometry, Mouse Assay, Microscopy

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    Thermo Fisher mhc class i
    Recombinant Rv2299c induces DC maturation A . Purified recombinant Rv2299c protein was analyzed by SDS-PAGE with Coomassie blue staining (a) and western blot analysis using anti-His antibodies (b). DCs were activated with the indicated concentration of Rv2299c or LPS (100 ng/ml) for 24 hr. B . Activated DCs were incubated with dextran-FITC at 37°C or 4°C for 30 min and assessed by FACS analysis of dextran (FITC) uptake. The percentages of dextran (FITC)-positive CD11c (PE)-positive cells are indicated. The results are representative of the results of four experiments. Bar graphs show mean ± SEM of four experiments. C . Activated DCs were stained with an anti-CD80, anti-CD86, <t>anti-MHC</t> class I, or anti-MHC class II Ab and analyzed for expression of surface markers. Bar graphs show the percentages (mean ± SEM of five separate experiments) for each surface molecule on CD11c+ cells. D . TNF-α, IL-6, IL-1β, IL-12p70, and IL-10 levels in the culture medium were measured by an ELISA. Data are presented as mean ± SEM (n = 5). E . Dot plots of intracellular IL-12p70 and IL-10 expression in CD11c + DCs. * p
    Mhc Class I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher mouse anti rat mhc ii
    Immunofluorescence staining of differentiation, proliferation, and immune antigen marker expression of transplanted MSCs. Rats were euthanized at 7, 14 and 28 days post-transplantation and their lungs and pancreas were obtained in the TVT and PST groups, respectively. Representative micrographs illustrate the expression of GFP (green, transplanted cells), immune antigens (red, CD86 and <t>MHC</t> II), a proliferation marker (red, <t>PCNA)</t> and a differentiation marker (red, insulin) in the lung and pancreas tissues of recipients. GFP + MSCs were visible in all samples. By day 7, the expression of CD86, MHC II, PCNA and insulin was negative ( a ). However, expression of MHC II ( b ) and insulin ( c ) was observed at 14 and 28 days post-transplantation. Blue indicates nuclear staining with DAPI. MSC, mesenchymal stem cell; PST, pancreas subcapsular transplantation; TVT, tail vein transplantation.
    Mouse Anti Rat Mhc Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher major histocompatibility complex class ii mhcii
    Prostate-specific Gr-1 + <t>CD11b</t> + cell expansion is associated with suppression of dendritic cell and macrophage maturation. (A, left) Mature CD11c + <t>MHCII</t> + dendritic cells are present in both wild-type and mutant prostates, but their levels decrease precipitously
    Major Histocompatibility Complex Class Ii Mhcii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher anti canine mhc ii
    Flow cytometry results for expression of CD45, CD3, CD21, <t>CD79a,</t> CD14, CD172a, CD11c, <t>MHC</t> I, and MHC II. Expression of MHC II was also evaluated on cells treated with either LPS or IFNγ for 24 and 48 h. (BD cell line shown in red, isotype control in blue)
    Anti Canine Mhc Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Recombinant Rv2299c induces DC maturation A . Purified recombinant Rv2299c protein was analyzed by SDS-PAGE with Coomassie blue staining (a) and western blot analysis using anti-His antibodies (b). DCs were activated with the indicated concentration of Rv2299c or LPS (100 ng/ml) for 24 hr. B . Activated DCs were incubated with dextran-FITC at 37°C or 4°C for 30 min and assessed by FACS analysis of dextran (FITC) uptake. The percentages of dextran (FITC)-positive CD11c (PE)-positive cells are indicated. The results are representative of the results of four experiments. Bar graphs show mean ± SEM of four experiments. C . Activated DCs were stained with an anti-CD80, anti-CD86, anti-MHC class I, or anti-MHC class II Ab and analyzed for expression of surface markers. Bar graphs show the percentages (mean ± SEM of five separate experiments) for each surface molecule on CD11c+ cells. D . TNF-α, IL-6, IL-1β, IL-12p70, and IL-10 levels in the culture medium were measured by an ELISA. Data are presented as mean ± SEM (n = 5). E . Dot plots of intracellular IL-12p70 and IL-10 expression in CD11c + DCs. * p

    Journal: Oncotarget

    Article Title: Rv2299c, a novel dendritic cell-activating antigen of Mycobacterium tuberculosis, fused-ESAT-6 subunit vaccine confers improved and durable protection against the hypervirulent strain HN878 in mice

    doi: 10.18632/oncotarget.15256

    Figure Lengend Snippet: Recombinant Rv2299c induces DC maturation A . Purified recombinant Rv2299c protein was analyzed by SDS-PAGE with Coomassie blue staining (a) and western blot analysis using anti-His antibodies (b). DCs were activated with the indicated concentration of Rv2299c or LPS (100 ng/ml) for 24 hr. B . Activated DCs were incubated with dextran-FITC at 37°C or 4°C for 30 min and assessed by FACS analysis of dextran (FITC) uptake. The percentages of dextran (FITC)-positive CD11c (PE)-positive cells are indicated. The results are representative of the results of four experiments. Bar graphs show mean ± SEM of four experiments. C . Activated DCs were stained with an anti-CD80, anti-CD86, anti-MHC class I, or anti-MHC class II Ab and analyzed for expression of surface markers. Bar graphs show the percentages (mean ± SEM of five separate experiments) for each surface molecule on CD11c+ cells. D . TNF-α, IL-6, IL-1β, IL-12p70, and IL-10 levels in the culture medium were measured by an ELISA. Data are presented as mean ± SEM (n = 5). E . Dot plots of intracellular IL-12p70 and IL-10 expression in CD11c + DCs. * p

    Article Snippet: The FITC-conjugated mAbs against CD11c, p65, IFN-γ, and CD62L, the APC-conjugated mAbs against IL-12p70, IL-10, and CD3, the PerCP-Cy5.5-conjugated mAb against CD4 and CD8, the APC-Cy7-conjugated mAb against CD8+ , the phycoerythrin (PE)-conjugated mAbs against CD80, CD86, MHC class I, MHC class II, IFN-γ, and CD44, the PE-Cy7-conjugated mAbs against CD11c and IL-2, and the eFluor® 450-conjugated mAb against CD3e were purchased from eBioscience (San Diego, CA, USA).

    Techniques: Recombinant, Purification, SDS Page, Staining, Western Blot, Concentration Assay, Incubation, FACS, Expressing, Enzyme-linked Immunosorbent Assay

    Immunofluorescence staining of differentiation, proliferation, and immune antigen marker expression of transplanted MSCs. Rats were euthanized at 7, 14 and 28 days post-transplantation and their lungs and pancreas were obtained in the TVT and PST groups, respectively. Representative micrographs illustrate the expression of GFP (green, transplanted cells), immune antigens (red, CD86 and MHC II), a proliferation marker (red, PCNA) and a differentiation marker (red, insulin) in the lung and pancreas tissues of recipients. GFP + MSCs were visible in all samples. By day 7, the expression of CD86, MHC II, PCNA and insulin was negative ( a ). However, expression of MHC II ( b ) and insulin ( c ) was observed at 14 and 28 days post-transplantation. Blue indicates nuclear staining with DAPI. MSC, mesenchymal stem cell; PST, pancreas subcapsular transplantation; TVT, tail vein transplantation.

    Journal: Cellular and Molecular Immunology

    Article Title: Immunogenicity of allogeneic mesenchymal stem cells transplanted via different routes in diabetic rats

    doi: 10.1038/cmi.2014.70

    Figure Lengend Snippet: Immunofluorescence staining of differentiation, proliferation, and immune antigen marker expression of transplanted MSCs. Rats were euthanized at 7, 14 and 28 days post-transplantation and their lungs and pancreas were obtained in the TVT and PST groups, respectively. Representative micrographs illustrate the expression of GFP (green, transplanted cells), immune antigens (red, CD86 and MHC II), a proliferation marker (red, PCNA) and a differentiation marker (red, insulin) in the lung and pancreas tissues of recipients. GFP + MSCs were visible in all samples. By day 7, the expression of CD86, MHC II, PCNA and insulin was negative ( a ). However, expression of MHC II ( b ) and insulin ( c ) was observed at 14 and 28 days post-transplantation. Blue indicates nuclear staining with DAPI. MSC, mesenchymal stem cell; PST, pancreas subcapsular transplantation; TVT, tail vein transplantation.

    Article Snippet: The sections were then incubated with a rabbit anti-GFP antibody (1∶800; Abcam, Cambridge, UK) either alone or with mouse anti-rat PCNA (GeneTex, Alton PkwyIrvine, CA, USA), mouse anti-rat MHC II (1∶200; eBioscience), mouse anti-rat CD86 (1∶200; eBioscience) or mouse anti-rat insulin (1∶400; Abcam) antibodies at 4 °C overnight.

    Techniques: Immunofluorescence, Staining, Marker, Expressing, Transplantation Assay

    Adipogenesis and osteogenesis of MSCs and immunogenicity testing in vitro . Cells were spindle-shaped after three passages and transduction with a GFP expression vector ( aI and II ). MSCs were then cultured in appropriate induction media for 3 weeks. Adipogenesis was confirmed by Oil Red O staining and osteogenesis was confirmed by Alizarin red staining ( aIII and IV , respectively). FACS showed that P7 MSCs expressed a low level of MHC I and did not express MHC II, CD40, CD80 or CD86 ( b ). PBMCs from normal and diabetic rats were cocultured with MSCs for 72 h. No proliferation was observed in the MSC group compared with the autoproliferation and ConA (positive) group ( c ). ** P

    Journal: Cellular and Molecular Immunology

    Article Title: Immunogenicity of allogeneic mesenchymal stem cells transplanted via different routes in diabetic rats

    doi: 10.1038/cmi.2014.70

    Figure Lengend Snippet: Adipogenesis and osteogenesis of MSCs and immunogenicity testing in vitro . Cells were spindle-shaped after three passages and transduction with a GFP expression vector ( aI and II ). MSCs were then cultured in appropriate induction media for 3 weeks. Adipogenesis was confirmed by Oil Red O staining and osteogenesis was confirmed by Alizarin red staining ( aIII and IV , respectively). FACS showed that P7 MSCs expressed a low level of MHC I and did not express MHC II, CD40, CD80 or CD86 ( b ). PBMCs from normal and diabetic rats were cocultured with MSCs for 72 h. No proliferation was observed in the MSC group compared with the autoproliferation and ConA (positive) group ( c ). ** P

    Article Snippet: The sections were then incubated with a rabbit anti-GFP antibody (1∶800; Abcam, Cambridge, UK) either alone or with mouse anti-rat PCNA (GeneTex, Alton PkwyIrvine, CA, USA), mouse anti-rat MHC II (1∶200; eBioscience), mouse anti-rat CD86 (1∶200; eBioscience) or mouse anti-rat insulin (1∶400; Abcam) antibodies at 4 °C overnight.

    Techniques: In Vitro, Transduction, Expressing, Plasmid Preparation, Cell Culture, Staining, FACS

    Prostate-specific Gr-1 + CD11b + cell expansion is associated with suppression of dendritic cell and macrophage maturation. (A, left) Mature CD11c + MHCII + dendritic cells are present in both wild-type and mutant prostates, but their levels decrease precipitously

    Journal: Molecular and Cellular Biology

    Article Title: Pten Null Prostate Epithelium Promotes Localized Myeloid-Derived Suppressor Cell Expansion and Immune Suppression during Tumor Initiation and Progression

    doi: 10.1128/MCB.00090-14

    Figure Lengend Snippet: Prostate-specific Gr-1 + CD11b + cell expansion is associated with suppression of dendritic cell and macrophage maturation. (A, left) Mature CD11c + MHCII + dendritic cells are present in both wild-type and mutant prostates, but their levels decrease precipitously

    Article Snippet: Single-cell suspensions were stained with directly conjugated antibodies against CD45, Gr-1, CD11b, CD4, CD8, CD69, B220 (BD Biosciences), F4/80, CD19, CD11c, major histocompatibility complex class II (MHCII) (eBioscience), and Ly6C (BioLegend), according to the manufacturers' instructions.

    Techniques: Mutagenesis

    Flow cytometry results for expression of CD45, CD3, CD21, CD79a, CD14, CD172a, CD11c, MHC I, and MHC II. Expression of MHC II was also evaluated on cells treated with either LPS or IFNγ for 24 and 48 h. (BD cell line shown in red, isotype control in blue)

    Journal: BMC Cancer

    Article Title: A novel canine histiocytic sarcoma cell line: initial characterization and utilization for drug screening studies

    doi: 10.1186/s12885-018-4132-0

    Figure Lengend Snippet: Flow cytometry results for expression of CD45, CD3, CD21, CD79a, CD14, CD172a, CD11c, MHC I, and MHC II. Expression of MHC II was also evaluated on cells treated with either LPS or IFNγ for 24 and 48 h. (BD cell line shown in red, isotype control in blue)

    Article Snippet: BD cells harvested from cell culture were labeled with the following monoclonal antibodies: anti-canine CD3 (CA17.2A12, Serotec), anti-canine CD11c (CA11.6A1, UC Davis/NIH NeuroMab Facility), anti-bovine CD14 (MM61A, WSU MAC), anti-canine CD21 (CA2.1D6, Serotec), anti-canine CD45 (YKIX716.13, Serotec), anti-canine CD79a (HM57, LS Bio), anti-bovine CD172a (DH59B, WSU MAC), anti-canine MHC II (YKIX334.2, eBioscience), and anti-bovine MHC I (MHC CL I, WSU MAC).

    Techniques: Flow Cytometry, Cytometry, Expressing