anti mglur1  (Alomone Labs)


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    Alomone Labs anti mglur1
    Dispersal of synaptic <t>mGluR1</t> clusters by Aβ requires NMDAR and VDCC activity. Rat cortical neurons were treated with Aβ (1 µM) for 1 h (with or without co-treatment with the NMDAR antagonist MK801, or the VDCC blocker verapamil) and then immunostained for surface mGluR1 before fixation. (A, B) Aβ downregulates surface mGluR1 (surface cluster size 66.0±2.8%, N = 10, n = 300, p
    Anti Mglur1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mglur1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mglur1 - by Bioz Stars, 2022-05
    93/100 stars

    Images

    1) Product Images from "Disassembly of Shank and Homer Synaptic Clusters Is Driven by Soluble ?-Amyloid1-40 through Divergent NMDAR-Dependent Signalling Pathways"

    Article Title: Disassembly of Shank and Homer Synaptic Clusters Is Driven by Soluble ?-Amyloid1-40 through Divergent NMDAR-Dependent Signalling Pathways

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0006011

    Dispersal of synaptic mGluR1 clusters by Aβ requires NMDAR and VDCC activity. Rat cortical neurons were treated with Aβ (1 µM) for 1 h (with or without co-treatment with the NMDAR antagonist MK801, or the VDCC blocker verapamil) and then immunostained for surface mGluR1 before fixation. (A, B) Aβ downregulates surface mGluR1 (surface cluster size 66.0±2.8%, N = 10, n = 300, p
    Figure Legend Snippet: Dispersal of synaptic mGluR1 clusters by Aβ requires NMDAR and VDCC activity. Rat cortical neurons were treated with Aβ (1 µM) for 1 h (with or without co-treatment with the NMDAR antagonist MK801, or the VDCC blocker verapamil) and then immunostained for surface mGluR1 before fixation. (A, B) Aβ downregulates surface mGluR1 (surface cluster size 66.0±2.8%, N = 10, n = 300, p

    Techniques Used: Activity Assay

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    Alomone Labs anti mglur1
    Dispersal of synaptic <t>mGluR1</t> clusters by Aβ requires NMDAR and VDCC activity. Rat cortical neurons were treated with Aβ (1 µM) for 1 h (with or without co-treatment with the NMDAR antagonist MK801, or the VDCC blocker verapamil) and then immunostained for surface mGluR1 before fixation. (A, B) Aβ downregulates surface mGluR1 (surface cluster size 66.0±2.8%, N = 10, n = 300, p
    Anti Mglur1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mglur1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mglur1 - by Bioz Stars, 2022-05
    93/100 stars
      Buy from Supplier

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    Dispersal of synaptic mGluR1 clusters by Aβ requires NMDAR and VDCC activity. Rat cortical neurons were treated with Aβ (1 µM) for 1 h (with or without co-treatment with the NMDAR antagonist MK801, or the VDCC blocker verapamil) and then immunostained for surface mGluR1 before fixation. (A, B) Aβ downregulates surface mGluR1 (surface cluster size 66.0±2.8%, N = 10, n = 300, p

    Journal: PLoS ONE

    Article Title: Disassembly of Shank and Homer Synaptic Clusters Is Driven by Soluble ?-Amyloid1-40 through Divergent NMDAR-Dependent Signalling Pathways

    doi: 10.1371/journal.pone.0006011

    Figure Lengend Snippet: Dispersal of synaptic mGluR1 clusters by Aβ requires NMDAR and VDCC activity. Rat cortical neurons were treated with Aβ (1 µM) for 1 h (with or without co-treatment with the NMDAR antagonist MK801, or the VDCC blocker verapamil) and then immunostained for surface mGluR1 before fixation. (A, B) Aβ downregulates surface mGluR1 (surface cluster size 66.0±2.8%, N = 10, n = 300, p

    Article Snippet: Cells were incubated with anti-mGluR1 (1∶100, Alomone laboratories, Jerusalem, Israel) for 1 h at 25°C and with Alexa-488 conjugated goat anti-rabbit IgG (1∶500).

    Techniques: Activity Assay

    Blocking mGluR5 with MPEP increases inspiratory neuron burst irregularity and decreases neuron burst frequency while additionally blocking mGluR1 with LY367385 does not further alter these parameters

    Journal: The European Journal of Neuroscience

    Article Title: Metabotropic glutamate receptors (mGluR5) activate TRPC channels to improve the regularity of the respiratory rhythm generated by the pre-B?tzinger Complex in mice

    doi: 10.1111/j.1460-9568.2012.08091.x

    Figure Lengend Snippet: Blocking mGluR5 with MPEP increases inspiratory neuron burst irregularity and decreases neuron burst frequency while additionally blocking mGluR1 with LY367385 does not further alter these parameters

    Article Snippet: The membranes were blocked overnight at +4°C with 2% non-fat dried milk (NFDM) (Bio-Rad Labs, Hercules, CA, USA) and 2% BSA (Sigma Aldrich, Milwaukee, WI, USA) in Tris buffered saline, pH = 7.5, containing 0.1% Tween-20 (TBS-T) buffer and immuno-blotted for 2h at room temperature with either anti-mGluR5 antibody (1:400) (Abcam, Cambridge, MA, USA), anti-mGluR1 antibody (1:800) (Alomone labs, Jerusalem, Israel), or anti-GAPDH antibody (1:1000, Abcam, USA) in 2% non-fat dried milk in TBS-T buffer.

    Techniques: Blocking Assay

    GRM1 and mGluR1 expression in TNBC. ( A ) Knockdown of GRM1 was accomplished by infecting MDA-MB-231 cells with GIPZ shRNA Lentiviral vectors containing a puromycin resistance gene and shRNA against GRM1 or a non-silencing shRNA construct (NS). GRM1 overexpression was accomplished by infecting MDA-MB-468 cells with pLenti6.3/V5-TOPO vectors containing a blasticidin resistance gene and GRM1 or LACZ construct ( LACZ ). Cells were stably selected with puromycin (1 μg/ml) or blasticidin (5 μg/ml) for 7 days and levels of GRM1 message ( A ) or its corresponding protein, mGluR1 ( B ) were measured by QPCR or Western blot, respectively. mGluR1 expression in SUM159, BT549 and non-transduced MDA-MB-231 TNBC cell lines were also detected. Results in A represent n = 2 experiments and are expressed as the mean ± SEM where *is P

    Journal: Scientific Reports

    Article Title: Metabotropic glutamate receptor-1 regulates inflammation in triple negative breast cancer

    doi: 10.1038/s41598-018-34502-8

    Figure Lengend Snippet: GRM1 and mGluR1 expression in TNBC. ( A ) Knockdown of GRM1 was accomplished by infecting MDA-MB-231 cells with GIPZ shRNA Lentiviral vectors containing a puromycin resistance gene and shRNA against GRM1 or a non-silencing shRNA construct (NS). GRM1 overexpression was accomplished by infecting MDA-MB-468 cells with pLenti6.3/V5-TOPO vectors containing a blasticidin resistance gene and GRM1 or LACZ construct ( LACZ ). Cells were stably selected with puromycin (1 μg/ml) or blasticidin (5 μg/ml) for 7 days and levels of GRM1 message ( A ) or its corresponding protein, mGluR1 ( B ) were measured by QPCR or Western blot, respectively. mGluR1 expression in SUM159, BT549 and non-transduced MDA-MB-231 TNBC cell lines were also detected. Results in A represent n = 2 experiments and are expressed as the mean ± SEM where *is P

    Article Snippet: Immunodetection of mGluR1 was performed using anti-mGluR1 antibody (Alamone Labs, Jerusalum, Israel) with appropriate secondary antibody and detected by chemiluminescence.

    Techniques: Expressing, Multiple Displacement Amplification, shRNA, Construct, Over Expression, Stable Transfection, Real-time Polymerase Chain Reaction, Western Blot

    mGluR-LTD induces loss of mushroom spines through activation of mGluR1. A. Representative images of hippocampal neurons treated with vehicle or DHPG in the presence of Bay (30 min recovery) and labeled with DiIC 18 ; arrowheads indicate mushroom spines, scale bars, 10 µm. B. Quantification of mushroom spine density from images like those in (a): mean ± SD, vehicle 1.7 ± 0.50 (n=55), DHPG+Bay 1.5 ± 0.57 (n=49), N=25 cells per group, p =0.1044, two-sided permutation t test. C. Representative images of hippocampal neurons treated with vehicle or DHPG in the presence of MPEP (30 min recovery) and labeled with DiIC 18 ; scale bars, 10 µm. D. Quantification of mushroom spine density from images like those in (c): mean ± SD, vehicle 1.7 ± 0.57 (n=53), DHPG+MPEP 1.3 ± 0.49 (n=40) from 3 experiments, p =0.0032, two-sided permutation t test.

    Journal: bioRxiv

    Article Title: Stabilization of spine Synaptopodin by mGluR1 is required for mGluR-LTD

    doi: 10.1101/2021.09.14.460352

    Figure Lengend Snippet: mGluR-LTD induces loss of mushroom spines through activation of mGluR1. A. Representative images of hippocampal neurons treated with vehicle or DHPG in the presence of Bay (30 min recovery) and labeled with DiIC 18 ; arrowheads indicate mushroom spines, scale bars, 10 µm. B. Quantification of mushroom spine density from images like those in (a): mean ± SD, vehicle 1.7 ± 0.50 (n=55), DHPG+Bay 1.5 ± 0.57 (n=49), N=25 cells per group, p =0.1044, two-sided permutation t test. C. Representative images of hippocampal neurons treated with vehicle or DHPG in the presence of MPEP (30 min recovery) and labeled with DiIC 18 ; scale bars, 10 µm. D. Quantification of mushroom spine density from images like those in (c): mean ± SD, vehicle 1.7 ± 0.57 (n=53), DHPG+MPEP 1.3 ± 0.49 (n=40) from 3 experiments, p =0.0032, two-sided permutation t test.

    Article Snippet: The following primary antibodies were used: rabbit anti-Synaptopodin (1/1000), goat anti-Synaptopodin (1/200; Santa Cruz Biotech, RRID: AB_2201166), rabbit mGluR1 (1/500, Alomone labs, RRID:AB_2039984; 1/10,000 RRID:AB_2571736), mouse anti-PSD95 (1/400; Antibodies Inc., RRID: AB_2292909), mouse anti-γ-Tubulin (1/2,500; Sigma Aldrich, RRID: AB_477584).

    Techniques: Activation Assay, Labeling

    Reduced density and growth of mushroom spines in mice lacking mGluR1. A. Representative images of DiIC 18 -labeled dendritic branches in the brain cortex of adult Grm1 WT and Grm1 KO mice; scale bars 10 µm. B. Quantification of spine density per 10 µm in the cortex of Grm1 WT and Grm1 KO mice from images as in (a). Juvenile (∼1.5 month-old), mean ± SD thin spines WT 6.8 ± 1.7 (n=32 dendritic branches), KO 7.8 ± 1.9 (n=25), (*) p =0.025; mushroom spines WT 1.9 ± 0.98 (n=34), KO 1.10 ± 0.39 (n=19), N=2 mice per group; (***) p

    Journal: bioRxiv

    Article Title: Stabilization of spine Synaptopodin by mGluR1 is required for mGluR-LTD

    doi: 10.1101/2021.09.14.460352

    Figure Lengend Snippet: Reduced density and growth of mushroom spines in mice lacking mGluR1. A. Representative images of DiIC 18 -labeled dendritic branches in the brain cortex of adult Grm1 WT and Grm1 KO mice; scale bars 10 µm. B. Quantification of spine density per 10 µm in the cortex of Grm1 WT and Grm1 KO mice from images as in (a). Juvenile (∼1.5 month-old), mean ± SD thin spines WT 6.8 ± 1.7 (n=32 dendritic branches), KO 7.8 ± 1.9 (n=25), (*) p =0.025; mushroom spines WT 1.9 ± 0.98 (n=34), KO 1.10 ± 0.39 (n=19), N=2 mice per group; (***) p

    Article Snippet: The following primary antibodies were used: rabbit anti-Synaptopodin (1/1000), goat anti-Synaptopodin (1/200; Santa Cruz Biotech, RRID: AB_2201166), rabbit mGluR1 (1/500, Alomone labs, RRID:AB_2039984; 1/10,000 RRID:AB_2571736), mouse anti-PSD95 (1/400; Antibodies Inc., RRID: AB_2292909), mouse anti-γ-Tubulin (1/2,500; Sigma Aldrich, RRID: AB_477584).

    Techniques: Mouse Assay, Labeling

    mGluR1 enables Synpo stabilization at excitatory spine synapses. A. Synpo co-precipitates with mGluR1: representative immunoblot probed for Synpo of immunoprecipitation (Ip) with anti-mGluR1 or control IgG from lysates of brain cortex of adult WT mice. B. Representative images of hippocampal neurons labeled for mGluR1, Synpo and MAP2: mGluR1 puncta are found in apposition to Synpo. Scale bar 50 µm; inset, 10 µm. C. Co-precipitation of PSD95 with Synpo in Grm1 WT and Grm1 KO mice: representative blot probed for PSD95 of immunoprecipitation with anti-Synpo antibody from lysates of brain cortex of adult Grm1 WT and Grm1 KO mice. Input, 5% of Ip lysate; Tubulin (Tub), loading control. D. Quantification of PSD95 co-precipitation with Synpo from blots as in (b). Mean ± SE, KO/WT PSD95 0.54 ± 0.12, KO/WT Synpo 1.2 ± 0.16, N=3 mice per group, two-tailed unpaired t-test.

    Journal: bioRxiv

    Article Title: Stabilization of spine Synaptopodin by mGluR1 is required for mGluR-LTD

    doi: 10.1101/2021.09.14.460352

    Figure Lengend Snippet: mGluR1 enables Synpo stabilization at excitatory spine synapses. A. Synpo co-precipitates with mGluR1: representative immunoblot probed for Synpo of immunoprecipitation (Ip) with anti-mGluR1 or control IgG from lysates of brain cortex of adult WT mice. B. Representative images of hippocampal neurons labeled for mGluR1, Synpo and MAP2: mGluR1 puncta are found in apposition to Synpo. Scale bar 50 µm; inset, 10 µm. C. Co-precipitation of PSD95 with Synpo in Grm1 WT and Grm1 KO mice: representative blot probed for PSD95 of immunoprecipitation with anti-Synpo antibody from lysates of brain cortex of adult Grm1 WT and Grm1 KO mice. Input, 5% of Ip lysate; Tubulin (Tub), loading control. D. Quantification of PSD95 co-precipitation with Synpo from blots as in (b). Mean ± SE, KO/WT PSD95 0.54 ± 0.12, KO/WT Synpo 1.2 ± 0.16, N=3 mice per group, two-tailed unpaired t-test.

    Article Snippet: The following primary antibodies were used: rabbit anti-Synaptopodin (1/1000), goat anti-Synaptopodin (1/200; Santa Cruz Biotech, RRID: AB_2201166), rabbit mGluR1 (1/500, Alomone labs, RRID:AB_2039984; 1/10,000 RRID:AB_2571736), mouse anti-PSD95 (1/400; Antibodies Inc., RRID: AB_2292909), mouse anti-γ-Tubulin (1/2,500; Sigma Aldrich, RRID: AB_477584).

    Techniques: Immunoprecipitation, Mouse Assay, Labeling, Two Tailed Test