anti megalin antibody (Alomone Labs)


Structured Review

Anti Megalin Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti megalin antibody/product/Alomone Labs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Kidney Specific Protein-Positive Cells Derived from Embryonic Stem Cells Reproduce Tubular Structures In Vitro and Differentiate into Renal Tubular Cells"
Article Title: Kidney Specific Protein-Positive Cells Derived from Embryonic Stem Cells Reproduce Tubular Structures In Vitro and Differentiate into Renal Tubular Cells
Journal: PLoS ONE
doi: 10.1371/journal.pone.0064843

Figure Legend Snippet: (a) Mouse neonate kidney stained with our anti-KSP antibody. The left picture is a low magnification, and the right picture is a high magnification. A micron scale is shown at the lower right corner. (b) Mouse kidney stained with our anti-KSP antibody, anti-AQP1 antibody, anti-Megalin antibody, anti-E-cadherin antibody and anti-AQP2 antibody. Scale bar, 400 µm. (c) Mouse small intestine stained with anti-E-cadherin antibody and our anti-KSP antibody. Scale bar, 400 µm. (d) Mouse ES cells stained using our anti-KSP antibody and anti-E-cadherin antibody after 18 days of differentiation with Activin (10 ng/mL).
Techniques Used: Staining

Figure Legend Snippet: (a) PCR showing the expression of segment-specific genes of renal tubular cells. The samples were KSP-positive cells examined right after cell purification with flow cytometry, KSP-positive cells forming tubular structures co-cultured with NIH3T3-Wnt4, and NIH3T3-Wnt4 alone. The bands were quantified using ImageJ, and graphs normalized against GAPDH were shown. After sorting: a sample of cells collected right after cell sorting of KSP-positive cells. Tubular structures: a sample of KSP-positive cells that form tubular structures and feeder cells of NIH3T3-Wnt4. Feeder alone: a sample of feeder cells, NIH3T3-Wnt4. (b) Immunofluorescence showing Megalin, AQP2 and Podocalyxin in tubular structures formed by KSP-positive cells co-cultured with NIH3T3-Wnt4. A micron scale is shown at the lower right corner.
Techniques Used: Expressing, Purification, Flow Cytometry, Cell Culture, FACS, Immunofluorescence
anti megalin antibody (Alomone Labs)


Structured Review

Anti Megalin Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti megalin antibody/product/Alomone Labs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Kidney Specific Protein-Positive Cells Derived from Embryonic Stem Cells Reproduce Tubular Structures In Vitro and Differentiate into Renal Tubular Cells"
Article Title: Kidney Specific Protein-Positive Cells Derived from Embryonic Stem Cells Reproduce Tubular Structures In Vitro and Differentiate into Renal Tubular Cells
Journal: PLoS ONE
doi: 10.1371/journal.pone.0064843

Figure Legend Snippet: (a) Mouse neonate kidney stained with our anti-KSP antibody. The left picture is a low magnification, and the right picture is a high magnification. A micron scale is shown at the lower right corner. (b) Mouse kidney stained with our anti-KSP antibody, anti-AQP1 antibody, anti-Megalin antibody, anti-E-cadherin antibody and anti-AQP2 antibody. Scale bar, 400 µm. (c) Mouse small intestine stained with anti-E-cadherin antibody and our anti-KSP antibody. Scale bar, 400 µm. (d) Mouse ES cells stained using our anti-KSP antibody and anti-E-cadherin antibody after 18 days of differentiation with Activin (10 ng/mL).
Techniques Used: Staining

Figure Legend Snippet: (a) PCR showing the expression of segment-specific genes of renal tubular cells. The samples were KSP-positive cells examined right after cell purification with flow cytometry, KSP-positive cells forming tubular structures co-cultured with NIH3T3-Wnt4, and NIH3T3-Wnt4 alone. The bands were quantified using ImageJ, and graphs normalized against GAPDH were shown. After sorting: a sample of cells collected right after cell sorting of KSP-positive cells. Tubular structures: a sample of KSP-positive cells that form tubular structures and feeder cells of NIH3T3-Wnt4. Feeder alone: a sample of feeder cells, NIH3T3-Wnt4. (b) Immunofluorescence showing Megalin, AQP2 and Podocalyxin in tubular structures formed by KSP-positive cells co-cultured with NIH3T3-Wnt4. A micron scale is shown at the lower right corner.
Techniques Used: Expressing, Purification, Flow Cytometry, Cell Culture, FACS, Immunofluorescence
guinea pig anti megalin (Alomone Labs)


Structured Review
Guinea Pig Anti Megalin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/guinea pig anti megalin/product/Alomone Labs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
megalin (Alomone Labs)


Structured Review

Megalin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megalin/product/Alomone Labs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Reduced Notch Signaling Leads to Renal Cysts and Papillary Microadenomas"
Article Title: Reduced Notch Signaling Leads to Renal Cysts and Papillary Microadenomas
Journal: Journal of the American Society of Nephrology : JASN
doi: 10.1681/ASN.2009090925

Figure Legend Snippet: The mechanism driving epithelial stratification and cyst formation is not loss of apical-basal polarity or reduced TCF2/HNFβ1 expression. (A) Na+/K+-ATPase is expressed in the basolateral membrane of PT epithelia in P1 wild-type (RBP-J f/f) littermates. (B) RBP-J deficient PT epithelia lining the cysts retain basolateral expression of Na+/K+-ATPase, although some regions (marked by asterisks) show a reduction in Na+/K+-ATPase expression. Inset in B shows a magnified view of the cyst-lining epithelia in which the arrowhead points at a stratified epithelium that retains polarized expression of Na+/K+-ATPase. (C) Kidneys from P1 Rarb2-Cretg;RBP-Jf/f mice were stained for TCF2/HNF1β (red). Although TCF2/HNF1β is downregulated in some cyst-lining epithelia in Notch-deficient kidneys (box 1), TCF2/HNF1β was expressed in all normal diameter PTs (box 2) and in some cyst-lining epithelia (box 3) containing stratified epithelia (arrowhead in box 4). (D) E-cadherin expression is restricted to the basolateral membrane, with a strong punctate staining at the adherence junctions of PT cells that express megalin in the apical membrane of wild-type kidneys. The inset shows magnified PTs with an asterisk in the lumen of a PT composed of epithelia with properly polarized expression of E-cadherin and megalin. (E, F) In the 5-week-old Six2-GFP::Cretg;N2f/f kidneys, PT epithelia retain apical-basal polarity (asterisks), except for a few epithelia lining the cysts, which either lack apical megalin and have apical E-cadherin (arrow) or have colocalization of megalin and E-cadherin in apical surface (arrowhead). All scale bars are 100 μm.
Techniques Used: Expressing, Staining
megalin (Alomone Labs)


Structured Review

Megalin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megalin/product/Alomone Labs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Reduced Notch Signaling Leads to Renal Cysts and Papillary Microadenomas"
Article Title: Reduced Notch Signaling Leads to Renal Cysts and Papillary Microadenomas
Journal: Journal of the American Society of Nephrology : JASN
doi: 10.1681/ASN.2009090925

Figure Legend Snippet: The mechanism driving epithelial stratification and cyst formation is not loss of apical-basal polarity or reduced TCF2/HNFβ1 expression. (A) Na+/K+-ATPase is expressed in the basolateral membrane of PT epithelia in P1 wild-type (RBP-J f/f) littermates. (B) RBP-J deficient PT epithelia lining the cysts retain basolateral expression of Na+/K+-ATPase, although some regions (marked by asterisks) show a reduction in Na+/K+-ATPase expression. Inset in B shows a magnified view of the cyst-lining epithelia in which the arrowhead points at a stratified epithelium that retains polarized expression of Na+/K+-ATPase. (C) Kidneys from P1 Rarb2-Cretg;RBP-Jf/f mice were stained for TCF2/HNF1β (red). Although TCF2/HNF1β is downregulated in some cyst-lining epithelia in Notch-deficient kidneys (box 1), TCF2/HNF1β was expressed in all normal diameter PTs (box 2) and in some cyst-lining epithelia (box 3) containing stratified epithelia (arrowhead in box 4). (D) E-cadherin expression is restricted to the basolateral membrane, with a strong punctate staining at the adherence junctions of PT cells that express megalin in the apical membrane of wild-type kidneys. The inset shows magnified PTs with an asterisk in the lumen of a PT composed of epithelia with properly polarized expression of E-cadherin and megalin. (E, F) In the 5-week-old Six2-GFP::Cretg;N2f/f kidneys, PT epithelia retain apical-basal polarity (asterisks), except for a few epithelia lining the cysts, which either lack apical megalin and have apical E-cadherin (arrow) or have colocalization of megalin and E-cadherin in apical surface (arrowhead). All scale bars are 100 μm.
Techniques Used: Expressing, Staining
megalin (Alomone Labs)


Structured Review
Megalin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megalin/product/Alomone Labs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
anti megalin (Alomone Labs)


Structured Review
Anti Megalin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti megalin/product/Alomone Labs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
anti megalin (Alomone Labs)


Structured Review
Anti Megalin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti megalin/product/Alomone Labs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99