anti mbp  (Abcam)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Name:
    Anti Maltose Binding Protein antibody
    Description:

    Catalog Number:
    AB9084
    Price:
    None
    Buy from Supplier


    Structured Review

    Abcam anti mbp
    Loss of HMGNs affects oligodendrocyte development in mouse spinal cord. ( A–D ) Immunofluorescence showing decreased OLIG2 positive cells in both white matter ( A ) and gray matter ( C ) of DKO mouse spinal cords. ( B, D) Quantifications of four WT and four DKO mice. For each mouse, > 1500 cells from six (white matter) or four (gray matter) different regions of spinal cord sections were counted. ( E ) Immunofluorescence showing decreased <t>MBP</t> level in DKO spinal cords. ( F ) Quantification of panel J, eight WT and eight DKO images were quantified by ImageJ. ( G ) Western blots showing the expression of oligodendrocyte markers in WT and DKO mouse spinal cords. ( H ) Quantifications of panel L, expression levels normalized to <t>β-actin.</t> All the data are presented as mean ± SEM (* P

    https://www.bioz.com/result/anti mbp/product/Abcam
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mbp - by Bioz Stars, 2021-09
    95/100 stars

    Images

    1) Product Images from "Interplay between H1 and HMGN epigenetically regulates OLIG1 2 expression and oligodendrocyte differentiation"

    Article Title: Interplay between H1 and HMGN epigenetically regulates OLIG1 2 expression and oligodendrocyte differentiation

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkw1222

    Loss of HMGNs affects oligodendrocyte development in mouse spinal cord. ( A–D ) Immunofluorescence showing decreased OLIG2 positive cells in both white matter ( A ) and gray matter ( C ) of DKO mouse spinal cords. ( B, D) Quantifications of four WT and four DKO mice. For each mouse, > 1500 cells from six (white matter) or four (gray matter) different regions of spinal cord sections were counted. ( E ) Immunofluorescence showing decreased MBP level in DKO spinal cords. ( F ) Quantification of panel J, eight WT and eight DKO images were quantified by ImageJ. ( G ) Western blots showing the expression of oligodendrocyte markers in WT and DKO mouse spinal cords. ( H ) Quantifications of panel L, expression levels normalized to β-actin. All the data are presented as mean ± SEM (* P
    Figure Legend Snippet: Loss of HMGNs affects oligodendrocyte development in mouse spinal cord. ( A–D ) Immunofluorescence showing decreased OLIG2 positive cells in both white matter ( A ) and gray matter ( C ) of DKO mouse spinal cords. ( B, D) Quantifications of four WT and four DKO mice. For each mouse, > 1500 cells from six (white matter) or four (gray matter) different regions of spinal cord sections were counted. ( E ) Immunofluorescence showing decreased MBP level in DKO spinal cords. ( F ) Quantification of panel J, eight WT and eight DKO images were quantified by ImageJ. ( G ) Western blots showing the expression of oligodendrocyte markers in WT and DKO mouse spinal cords. ( H ) Quantifications of panel L, expression levels normalized to β-actin. All the data are presented as mean ± SEM (* P

    Techniques Used: Immunofluorescence, Mouse Assay, Western Blot, Expressing

    2) Product Images from "Sephin1, which prolongs the integrated stress response, is a promising therapeutic for multiple sclerosis"

    Article Title: Sephin1, which prolongs the integrated stress response, is a promising therapeutic for multiple sclerosis

    Journal: Brain

    doi: 10.1093/brain/awy322

    Combination treatment with Sephin1 and IFN-β alleviates and delays clinical symptoms of EAE as well as reduces the oligodendrocytes and myelin loss during EAE course. ( A ) Clinical scores of C57BL/6 female mice immunized with MOG 35-55 /CFA to induce chronic EAE, treated with vehicle ( n = 12), 5000 U of IFN-β ( n = 12), 8 mg/kg of Sephin1 or Sephin1 combined with 5000 U of IFNβ ( n = 10) daily from PID 7 to the end of the study. ( B ) Average onset of disease, peak of disease and average peak score of all treatment groups. ( C and D ) Immunofluorescent staining for TPPP (green) and MBP (red) of lumbar spinal cord sections from PID 17 ( C ) and PID 30 ( D ). Scale bar = 100 μm. ( E ) Quantification of cells positive for TPPP in the lesion areas. ( F ) Average percentage of demyelinated areas/white matter areas measured from MBP staining. * P
    Figure Legend Snippet: Combination treatment with Sephin1 and IFN-β alleviates and delays clinical symptoms of EAE as well as reduces the oligodendrocytes and myelin loss during EAE course. ( A ) Clinical scores of C57BL/6 female mice immunized with MOG 35-55 /CFA to induce chronic EAE, treated with vehicle ( n = 12), 5000 U of IFN-β ( n = 12), 8 mg/kg of Sephin1 or Sephin1 combined with 5000 U of IFNβ ( n = 10) daily from PID 7 to the end of the study. ( B ) Average onset of disease, peak of disease and average peak score of all treatment groups. ( C and D ) Immunofluorescent staining for TPPP (green) and MBP (red) of lumbar spinal cord sections from PID 17 ( C ) and PID 30 ( D ). Scale bar = 100 μm. ( E ) Quantification of cells positive for TPPP in the lesion areas. ( F ) Average percentage of demyelinated areas/white matter areas measured from MBP staining. * P

    Techniques Used: Mouse Assay, Staining

    3) Product Images from "AATYK is a Novel Regulator of Oligodendrocyte Differentiation and Myelination"

    Article Title: AATYK is a Novel Regulator of Oligodendrocyte Differentiation and Myelination

    Journal: Neuroscience Bulletin

    doi: 10.1007/s12264-018-0218-6

    AATYK knockdown does not affect the survival but maintains proliferation of OPCs. A Ki67 immunostaining showed there were more Ki67 + and GFP + co-labeled cells in the sh- AATYK -treated OPCs than in the control. B Percentages of Ki67 + and GFP + co-labelled OLs in GFP + cells among RNAi- AATYK cells were significantly higher than in the control. C – D Compared to control cells, the expression of MBP protein was significantly decreased in shRNA- AATYK -treated cells and there was no difference in caspase3 between the two groups. Scale bar, 100 μm.
    Figure Legend Snippet: AATYK knockdown does not affect the survival but maintains proliferation of OPCs. A Ki67 immunostaining showed there were more Ki67 + and GFP + co-labeled cells in the sh- AATYK -treated OPCs than in the control. B Percentages of Ki67 + and GFP + co-labelled OLs in GFP + cells among RNAi- AATYK cells were significantly higher than in the control. C – D Compared to control cells, the expression of MBP protein was significantly decreased in shRNA- AATYK -treated cells and there was no difference in caspase3 between the two groups. Scale bar, 100 μm.

    Techniques Used: Immunostaining, Labeling, Expressing, shRNA

    4) Product Images from "Intravenous C16 and angiopoietin-1 improve the efficacy of placenta-derived mesenchymal stem cell therapy for EAE"

    Article Title: Intravenous C16 and angiopoietin-1 improve the efficacy of placenta-derived mesenchymal stem cell therapy for EAE

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-22867-9

    Protein expression of MBP ( A – C ), NF-200 ( D – F ), p75NTR ( G – I ), BDNF ( J – L ), GAP-43 ( M – O ) and caspase-3 ( P – R ) in the brain cortex at 3 and 8 weeks pi by western blotting. n = 5. # P
    Figure Legend Snippet: Protein expression of MBP ( A – C ), NF-200 ( D – F ), p75NTR ( G – I ), BDNF ( J – L ), GAP-43 ( M – O ) and caspase-3 ( P – R ) in the brain cortex at 3 and 8 weeks pi by western blotting. n = 5. # P

    Techniques Used: Expressing, Western Blot

    5) Product Images from "Collagen-Binding Hepatocyte Growth Factor (HGF) alone or with a Gelatin- furfurylamine Hydrogel Enhances Functional Recovery in Mice after Spinal Cord Injury"

    Article Title: Collagen-Binding Hepatocyte Growth Factor (HGF) alone or with a Gelatin- furfurylamine Hydrogel Enhances Functional Recovery in Mice after Spinal Cord Injury

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-19316-y

    Axonal growth after spinal cord transection injury. Mice subjected to a transection injury were treated with gelatin-FA hydrogel alone, gelatin-FA hydrogel + CBD-HGF or gelatin-FA hydrogel + HGF. Untreated mice were used as controls (5 mice each per 4 groups). Immunohistochemistry for GAP43 ( a ), MBP ( b ) and GFAP ( c ) at the injury sites was performed 8 weeks post-injury. Left: Representative photographs from each group. The scale bar indicates 500 µm (dotted line) and 50 µm (solid line). Right: Immunopositive areas were measured under a microscope and percent area is shown. * p
    Figure Legend Snippet: Axonal growth after spinal cord transection injury. Mice subjected to a transection injury were treated with gelatin-FA hydrogel alone, gelatin-FA hydrogel + CBD-HGF or gelatin-FA hydrogel + HGF. Untreated mice were used as controls (5 mice each per 4 groups). Immunohistochemistry for GAP43 ( a ), MBP ( b ) and GFAP ( c ) at the injury sites was performed 8 weeks post-injury. Left: Representative photographs from each group. The scale bar indicates 500 µm (dotted line) and 50 µm (solid line). Right: Immunopositive areas were measured under a microscope and percent area is shown. * p

    Techniques Used: Mouse Assay, Immunohistochemistry, Microscopy

    Axonal growth after spinal cord compression injury. Mice subjected to a compression injury were treated with a single administration of CBD-HGF or HGF. Untreated mice were used as controls (4 mice each per 3 groups). Immunohistochemistry for GAP43 ( a ), MBP ( b ) and GFAP ( c ) at the injury sites was performed 6 weeks post-injury. Left: Representative photographs from each group. The scale bar indicates 500 µm (solid line) and 50 µm (dotted line). Right: Immunopositive areas were measured under a microscope and percent area is shown. * p
    Figure Legend Snippet: Axonal growth after spinal cord compression injury. Mice subjected to a compression injury were treated with a single administration of CBD-HGF or HGF. Untreated mice were used as controls (4 mice each per 3 groups). Immunohistochemistry for GAP43 ( a ), MBP ( b ) and GFAP ( c ) at the injury sites was performed 6 weeks post-injury. Left: Representative photographs from each group. The scale bar indicates 500 µm (solid line) and 50 µm (dotted line). Right: Immunopositive areas were measured under a microscope and percent area is shown. * p

    Techniques Used: Mouse Assay, Immunohistochemistry, Microscopy

    6) Product Images from "Human dental pulp-derived stem cells promote locomotor recovery after complete transection of the rat spinal cord by multiple neuro-regenerative mechanisms"

    Article Title: Human dental pulp-derived stem cells promote locomotor recovery after complete transection of the rat spinal cord by multiple neuro-regenerative mechanisms

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI59251

    SHEDs suppress the apoptosis of neural cell lineages and secondary injury after SCI. Representative images ( A – L ) and quantifications ( M and N ) of apoptotic cell death 24 hours after SCI. Transverse sections 1 mm caudal to the epicenter of PBS-injected ( A , B , E , F , I , and J ) and SHED-transplanted SCs ( C , D , G , H , K , and L ) were stained with TUNEL and then subjected to immunohistochemical analysis with an anti-GFAP mAb ( A – D ), anti-NeuN mAb ( E – H ), or anti-MBP mAb ( I – L ). The engrafted SHEDs decreased the apoptotic cell death of all 3 neural cell lineages. ( M ) Quantification of the total TUNEL-positive cell number within 3 mm rostral and caudal to the epicenter shows the average of 3 experiments performed in parallel. ( N ) The percentage of TUNEL-positive relative to total DAPI-positive cell number in the same area as in M . Error bars represent SD. * P
    Figure Legend Snippet: SHEDs suppress the apoptosis of neural cell lineages and secondary injury after SCI. Representative images ( A – L ) and quantifications ( M and N ) of apoptotic cell death 24 hours after SCI. Transverse sections 1 mm caudal to the epicenter of PBS-injected ( A , B , E , F , I , and J ) and SHED-transplanted SCs ( C , D , G , H , K , and L ) were stained with TUNEL and then subjected to immunohistochemical analysis with an anti-GFAP mAb ( A – D ), anti-NeuN mAb ( E – H ), or anti-MBP mAb ( I – L ). The engrafted SHEDs decreased the apoptotic cell death of all 3 neural cell lineages. ( M ) Quantification of the total TUNEL-positive cell number within 3 mm rostral and caudal to the epicenter shows the average of 3 experiments performed in parallel. ( N ) The percentage of TUNEL-positive relative to total DAPI-positive cell number in the same area as in M . Error bars represent SD. * P

    Techniques Used: Injection, Staining, TUNEL Assay, Immunohistochemistry

    7) Product Images from "Fat tissue, a potential Schwann cell reservoir: isolation and identification of adipose-derived Schwann cells"

    Article Title: Fat tissue, a potential Schwann cell reservoir: isolation and identification of adipose-derived Schwann cells

    Journal: American Journal of Translational Research

    doi:

    Regenerated sciatic nerves 6 months after surgery in the four groups. MBP (A-D, red), S100 (E-H, red), and NF-H (I-L, red) staining of regenerated sciatic nerves in the SCLC-L, SCLC-M, SCLC-H and SNSC. Green: GFP. Yellow: co-located site. Blue: DAPI.
    Figure Legend Snippet: Regenerated sciatic nerves 6 months after surgery in the four groups. MBP (A-D, red), S100 (E-H, red), and NF-H (I-L, red) staining of regenerated sciatic nerves in the SCLC-L, SCLC-M, SCLC-H and SNSC. Green: GFP. Yellow: co-located site. Blue: DAPI.

    Techniques Used: Staining

    Regenerated sciatic nerves 3 months after surgery in the four groups. MBP (A-D, red), S100 (E-H, red), and NF-H (I-L, red) staining of regenerated sciatic nerves in the SCLC-L, SCLC-M, SCLC-H and SNSC group. Green: GFP. Red: MBP, S100, and NF-H. Yellow:
    Figure Legend Snippet: Regenerated sciatic nerves 3 months after surgery in the four groups. MBP (A-D, red), S100 (E-H, red), and NF-H (I-L, red) staining of regenerated sciatic nerves in the SCLC-L, SCLC-M, SCLC-H and SNSC group. Green: GFP. Red: MBP, S100, and NF-H. Yellow:

    Techniques Used: Staining

    Detection and location of nerve tissues in mouse inguinal adipose tissue. (A, E, I) H E staining of inguinal adipose tissue in neonatal mice. SC markers (green) of Sox10 (B-D), P75 NTR (F-H) and MBP (J-L) were double-stained (yellow, arrow) with
    Figure Legend Snippet: Detection and location of nerve tissues in mouse inguinal adipose tissue. (A, E, I) H E staining of inguinal adipose tissue in neonatal mice. SC markers (green) of Sox10 (B-D), P75 NTR (F-H) and MBP (J-L) were double-stained (yellow, arrow) with

    Techniques Used: Staining, Mouse Assay

    8) Product Images from "Differentiation potential of neural stem cells derived from fetal sheep"

    Article Title: Differentiation potential of neural stem cells derived from fetal sheep

    Journal: Animal Cells and Systems

    doi: 10.1080/19768354.2017.1354915

    Neurotrophic factors promoted neuritogenesis and maturation of neurons and glia derived from NSCs after induced for 12 days in vitro. (A) and (B) GFAP expression and nuclear staining. GFAP as astrocyte maker. (C) Merger of (A) and (B). (D) MAP2, as neuronal marker. (F) Merger of (D) and (E). (G) MBP expression. MBP as oligodendrocyte maker. (I) Merger of (G) and (H). (E) and (H) Nuclear staining. Bar scales = 40 μm.
    Figure Legend Snippet: Neurotrophic factors promoted neuritogenesis and maturation of neurons and glia derived from NSCs after induced for 12 days in vitro. (A) and (B) GFAP expression and nuclear staining. GFAP as astrocyte maker. (C) Merger of (A) and (B). (D) MAP2, as neuronal marker. (F) Merger of (D) and (E). (G) MBP expression. MBP as oligodendrocyte maker. (I) Merger of (G) and (H). (E) and (H) Nuclear staining. Bar scales = 40 μm.

    Techniques Used: Derivative Assay, In Vitro, Expressing, Staining, Marker

    9) Product Images from "Galectin-1-secreting neural stem cells elicit long-term neuroprotection against ischemic brain injury"

    Article Title: Galectin-1-secreting neural stem cells elicit long-term neuroprotection against ischemic brain injury

    Journal: Scientific Reports

    doi: 10.1038/srep09621

    Transplantation of s-NSCs improves white matter integrity 28 days after ischemia. Mice received transplantation of o-NSCs or s-NSCs 2 hours after MCAO. Brain sections were dual-stained for myelin basic protein (MBP) and non-phosphorylated neurofilament H (SMI-32) on day 28 after ischemia. (a–b) Representative immunofluorescent images of MBP and SMI-32 staining in the corpus callosum (a) and striatum (b) after sham surgery or MCAO followed by transplantation of vehicle, o-NSCs, or s-NSCs. (c–d) Quantification of MBP and SMI-32 immunofluorescence in the corpus callosum (c) and striatum (d), expressed as percentages and folds of contralateral fluorescence intensities, respectively. Data are mean ± SEM, n = 6. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 versus sham; # p ≤ 0.05, ## p ≤ 0.01 versus vehicle; $ p ≤ 0.05 versus o-NSC.
    Figure Legend Snippet: Transplantation of s-NSCs improves white matter integrity 28 days after ischemia. Mice received transplantation of o-NSCs or s-NSCs 2 hours after MCAO. Brain sections were dual-stained for myelin basic protein (MBP) and non-phosphorylated neurofilament H (SMI-32) on day 28 after ischemia. (a–b) Representative immunofluorescent images of MBP and SMI-32 staining in the corpus callosum (a) and striatum (b) after sham surgery or MCAO followed by transplantation of vehicle, o-NSCs, or s-NSCs. (c–d) Quantification of MBP and SMI-32 immunofluorescence in the corpus callosum (c) and striatum (d), expressed as percentages and folds of contralateral fluorescence intensities, respectively. Data are mean ± SEM, n = 6. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 versus sham; # p ≤ 0.05, ## p ≤ 0.01 versus vehicle; $ p ≤ 0.05 versus o-NSC.

    Techniques Used: Transplantation Assay, Mouse Assay, Staining, Immunofluorescence, Fluorescence

    10) Product Images from "Specific Marker Expression and Cell State of Schwann Cells during Culture In Vitro"

    Article Title: Specific Marker Expression and Cell State of Schwann Cells during Culture In Vitro

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0123278

    The expression of Schwann cell markers in murine sciatic nerve. Immunofluorescence staining of sciatic nerve vertical section from newborn (A-J) and adult (a-j) mice. (A, a) S100; (B, b) Sox10; (C, c)Oct6 failed to be detected in adult SN whereas newborn SN is Oct6 positive; (D, d) Krox20; (E, e)Sox2 was not expressed in both newborn and adult SN; (F, f) P75 NTR ; (G, g)NCAM; (H, h)GAP43; (I, i) MBP; (J, j) MPZ. (K) Real-time quantitative RT-PCR analysis of mRNA expression in newborn and adult mice SN. S100 mRNA level in adult SN increased while that of Sox10, Oct6, P75 NTR , NCAM, GAP43 decreased during culture in vitro . * P
    Figure Legend Snippet: The expression of Schwann cell markers in murine sciatic nerve. Immunofluorescence staining of sciatic nerve vertical section from newborn (A-J) and adult (a-j) mice. (A, a) S100; (B, b) Sox10; (C, c)Oct6 failed to be detected in adult SN whereas newborn SN is Oct6 positive; (D, d) Krox20; (E, e)Sox2 was not expressed in both newborn and adult SN; (F, f) P75 NTR ; (G, g)NCAM; (H, h)GAP43; (I, i) MBP; (J, j) MPZ. (K) Real-time quantitative RT-PCR analysis of mRNA expression in newborn and adult mice SN. S100 mRNA level in adult SN increased while that of Sox10, Oct6, P75 NTR , NCAM, GAP43 decreased during culture in vitro . * P

    Techniques Used: Expressing, Immunofluorescence, Staining, Mouse Assay, Quantitative RT-PCR, In Vitro

    11) Product Images from "LINGO-1 antibody ameliorates myelin impairment and spatial memory deficits in experimental autoimmune encephalomyelitis mice"

    Article Title: LINGO-1 antibody ameliorates myelin impairment and spatial memory deficits in experimental autoimmune encephalomyelitis mice

    Journal: Scientific Reports

    doi: 10.1038/srep14235

    LINGO-1 antibody promotes parahippocampal cortex (PHC) remyelination in EAE mice. ( a , b ) The EAE mice exhibited demyelination in the PHC and fimbria-fornix. After a six-week treatment with the LINGO-1 antibody, the level of MBP was significantly increased in the PHC, but no significant change was observed in the fimbria-fornix. ( c ) We found a severe reduction in the expression of kinesin light chain (KLC), in the PHC of the EAE mice compared with controls, and the KLC expression was restored in the anti-LINGO-1 treated mice. ( e )No significant differences were found for dynein (DYN), in the PHC of the EAE mice and the LINGO-1 antibody treated mice. ( g ) A reduction in the expression of neurofilament 200 (NF200), was detected in the EAE mice, and a slight increase was observed in the LINGO-1 antibody-treated mice. ( d , h ) KLC/NF200 expression was also decreased in the fimbria-fornix of the EAE but was not restored to normal levels in the EAE mice treated with the LINGO-1 antibody. ( f ) There was no significant difference in the expression of DYN among the three groups of mice. ( i – k ) Immunohistochemical staining for MBP in the three groups ( l ) Medial entorhinal cortex (MEnt) is an important subregion of the parahippocampal cortex. Schematic diagram display the scope we get the image in the microscope. ( j ) One-way ANOV A with least significance difference (LSD) test was used to determine statistical significance of MBP. The mean OD value was decline in the EAE mice and after LINGO-1 treatment, it was increased. n(Ctrl) = 3, n(EAE) = 4, n(Anti-LINGO-1) = 3. *Denotes statistical significance compared with controls (P
    Figure Legend Snippet: LINGO-1 antibody promotes parahippocampal cortex (PHC) remyelination in EAE mice. ( a , b ) The EAE mice exhibited demyelination in the PHC and fimbria-fornix. After a six-week treatment with the LINGO-1 antibody, the level of MBP was significantly increased in the PHC, but no significant change was observed in the fimbria-fornix. ( c ) We found a severe reduction in the expression of kinesin light chain (KLC), in the PHC of the EAE mice compared with controls, and the KLC expression was restored in the anti-LINGO-1 treated mice. ( e )No significant differences were found for dynein (DYN), in the PHC of the EAE mice and the LINGO-1 antibody treated mice. ( g ) A reduction in the expression of neurofilament 200 (NF200), was detected in the EAE mice, and a slight increase was observed in the LINGO-1 antibody-treated mice. ( d , h ) KLC/NF200 expression was also decreased in the fimbria-fornix of the EAE but was not restored to normal levels in the EAE mice treated with the LINGO-1 antibody. ( f ) There was no significant difference in the expression of DYN among the three groups of mice. ( i – k ) Immunohistochemical staining for MBP in the three groups ( l ) Medial entorhinal cortex (MEnt) is an important subregion of the parahippocampal cortex. Schematic diagram display the scope we get the image in the microscope. ( j ) One-way ANOV A with least significance difference (LSD) test was used to determine statistical significance of MBP. The mean OD value was decline in the EAE mice and after LINGO-1 treatment, it was increased. n(Ctrl) = 3, n(EAE) = 4, n(Anti-LINGO-1) = 3. *Denotes statistical significance compared with controls (P

    Techniques Used: Mouse Assay, Expressing, Immunohistochemistry, Staining, Microscopy

    12) Product Images from "Conditional Deletion of Foxg1 Alleviates Demyelination and Facilitates Remyelination via the Wnt Signaling Pathway in Cuprizone-Induced Demyelinated Mice"

    Article Title: Conditional Deletion of Foxg1 Alleviates Demyelination and Facilitates Remyelination via the Wnt Signaling Pathway in Cuprizone-Induced Demyelinated Mice

    Journal: Neuroscience Bulletin

    doi: 10.1007/s12264-020-00583-7

    Conditional knockout of Foxg1 promotes the differentiation of oligodendrocyte precursor cells in the corpus callosum (CC) of mice induced by CPZ. A Schematic of cuprizone (CPZ) and BrdU administration time points during the experiment. Adult 7-week-old mice were fed a diet containing 0.2% CPZ for 5 weeks (demyelination phase); Tamoxifen (TM) was intraperitoneally injected (3 times, 24 h apart) from day 0 after feeding on a CPZ diet; BrdU was intraperitoneally injected twice a day for 7 consecutive days, and the mice were sacrificed on day 35. B Image showing the measured region (black dashed box) in coronal sections as in C – H . C – H Immunofluorescence double-labeled staining and quantitative analysis of BrdU/MBP ( C , D ), BrdU/CNPase ( E , F ), and BrdU/MAG ( G , H ) in the CC of Foxg1- cKO mice. White arrows indicate representative double-labeled positive cells; n = 8 per group; data are presented as the mean ± SEM; ** P
    Figure Legend Snippet: Conditional knockout of Foxg1 promotes the differentiation of oligodendrocyte precursor cells in the corpus callosum (CC) of mice induced by CPZ. A Schematic of cuprizone (CPZ) and BrdU administration time points during the experiment. Adult 7-week-old mice were fed a diet containing 0.2% CPZ for 5 weeks (demyelination phase); Tamoxifen (TM) was intraperitoneally injected (3 times, 24 h apart) from day 0 after feeding on a CPZ diet; BrdU was intraperitoneally injected twice a day for 7 consecutive days, and the mice were sacrificed on day 35. B Image showing the measured region (black dashed box) in coronal sections as in C – H . C – H Immunofluorescence double-labeled staining and quantitative analysis of BrdU/MBP ( C , D ), BrdU/CNPase ( E , F ), and BrdU/MAG ( G , H ) in the CC of Foxg1- cKO mice. White arrows indicate representative double-labeled positive cells; n = 8 per group; data are presented as the mean ± SEM; ** P

    Techniques Used: Knock-Out, Mouse Assay, Injection, Immunofluorescence, Labeling, Staining

    13) Product Images from "Lactosylceramide synthases encoded by B4galt5 and 6 genes are pivotal for neuronal generation and myelin formation in mice"

    Article Title: Lactosylceramide synthases encoded by B4galt5 and 6 genes are pivotal for neuronal generation and myelin formation in mice

    Journal: PLoS Genetics

    doi: 10.1371/journal.pgen.1007545

    Immunohistochemical analysis of myelin-associated proteins and neurofilament (NF) in the cerebral cortex. (A–D) Immunohistochemical staining for MBP (A, red), MAG (B, red), MOG (C, red), and PLP (D, red) merged with NF (green) and DAPI (blue) in the cerebral cortices of wild-type (wt) and double knockout (DKO) mice at 3 weeks of age.
    Figure Legend Snippet: Immunohistochemical analysis of myelin-associated proteins and neurofilament (NF) in the cerebral cortex. (A–D) Immunohistochemical staining for MBP (A, red), MAG (B, red), MOG (C, red), and PLP (D, red) merged with NF (green) and DAPI (blue) in the cerebral cortices of wild-type (wt) and double knockout (DKO) mice at 3 weeks of age.

    Techniques Used: Immunohistochemistry, Staining, Plasmid Purification, Double Knockout, Mouse Assay

    Immunohistochemical and TEM analysis in the spinal cord. (A) Immunohistochemical staining for MBP, MAG, MOG, and PLP (red) merged with NF (green) in the spinal cords of wild-type (wt) and double knockout (DKO) mice at 3 weeks of age. (B) TEM analysis of the spinal cords of wt (upper) and DKO (lower) mice at 3 weeks of age. Right-hand pictures show a higher magnification of the squares in the left-hand pictures. Arrowheads: small axons without a myelin sheath; arrows: large axons with low circularity. (C) Frequency of axon circularity in wt (n = 1930) and DKO (n = 2120) mice. (D) The g-ratio (axon diameter/myelinated fiber diameter) of large axons of wt (n = 130) and DKO mice (n = 95) was measured. **, p
    Figure Legend Snippet: Immunohistochemical and TEM analysis in the spinal cord. (A) Immunohistochemical staining for MBP, MAG, MOG, and PLP (red) merged with NF (green) in the spinal cords of wild-type (wt) and double knockout (DKO) mice at 3 weeks of age. (B) TEM analysis of the spinal cords of wt (upper) and DKO (lower) mice at 3 weeks of age. Right-hand pictures show a higher magnification of the squares in the left-hand pictures. Arrowheads: small axons without a myelin sheath; arrows: large axons with low circularity. (C) Frequency of axon circularity in wt (n = 1930) and DKO (n = 2120) mice. (D) The g-ratio (axon diameter/myelinated fiber diameter) of large axons of wt (n = 130) and DKO mice (n = 95) was measured. **, p

    Techniques Used: Immunohistochemistry, Transmission Electron Microscopy, Staining, Plasmid Purification, Double Knockout, Mouse Assay

    14) Product Images from "TDP-43 Depletion in Microglia Promotes Amyloid Clearance but Also Induces Synapse Loss"

    Article Title: TDP-43 Depletion in Microglia Promotes Amyloid Clearance but Also Induces Synapse Loss

    Journal: Neuron

    doi: 10.1016/j.neuron.2017.05.037

    Selective Depletion of TDP-43 from Microglia Results in Enhanced Synaptic Loss in Mice Even in the Absence of Amyloid (A–E) Representative blots of synaptic markers in the motor/somatosensory cortex of WT and cKO 8-month-old mice (A) and relative quantification for vGlut-1 (B), PSD95 (C), MBP (D), and MAP2 (E) normalized to β-actin reference gene. Mean ± SEM, n = 3–4 mice per genotype, ∗ p
    Figure Legend Snippet: Selective Depletion of TDP-43 from Microglia Results in Enhanced Synaptic Loss in Mice Even in the Absence of Amyloid (A–E) Representative blots of synaptic markers in the motor/somatosensory cortex of WT and cKO 8-month-old mice (A) and relative quantification for vGlut-1 (B), PSD95 (C), MBP (D), and MAP2 (E) normalized to β-actin reference gene. Mean ± SEM, n = 3–4 mice per genotype, ∗ p

    Techniques Used: Mouse Assay

    15) Product Images from "Rapid identification of regulatory microRNAs by miTRAP (miRNA trapping by RNA in vitro affinity purification)"

    Article Title: Rapid identification of regulatory microRNAs by miTRAP (miRNA trapping by RNA in vitro affinity purification)

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gku127

    MiTRAP allows the selective co-purification of miRNAs with in vitro transcribed bait RNAs. ( A ) Scheme of the miTRAP procedure. In vitro transcribed bait RNAs comprising four MS2 stem-loops fused to the 3′end of bait transcripts were immobilized on amylose resin (light gray) via recombinant MBP-fused (dark gray) MS2-CP (white) protein. ( B ) Scheme of the used bait RNAs (upper panel). MS2: 120-nt-long control RNA encoded by the template vector; WT: wild type 3′UTR of the MYC mRNA; MUT: MYC-3′UTR with indicated point mutations (lower panel) in the overlapping MTS of the let-7-5p/miR-34-5p families. ( C ) Co-purification of miRNAs was determined by qRT-PCR and assessed by the miTRAP ratio that indicates the input normalized abundance of miRNAs in the miTRAP eluates (upper panel). MiTRAP ratios of indicated miRNAs were determined on co-purification with depicted RNA baits (B) from U2OS cell lysates. Ratios were determined by qRT-PCR using miRNA-specific TaqMan probes. Note that MYC-regulatory miRNAs of the let-7-5p and miR-34-5p families are selectively co-purified with the MYC-3′UTR bait RNA, but not the mutant 3′UTR or MS2 control transcript. MiTRAP ratios for the WT 3′UTR bait are two orders of magnitude (note logarithmic scale) above controls. Error bars indicate standard deviation (s.d.) of at least three independent analyses. Statistical significance was determined by Student’s t -test: * P ≤ 0.05. ( D ) Western blot analysis of indicated proteins isolated from U2OS input fractions or co-purified with MBP–MS2BP-coated amylose resin (BC), the MS2 control transcript (MS2), the WT or point mutated (MUT) MYC-3′UTR, respectively. TUBA4A served as negative control for unspecific binding, whereas MBP–MS2BP indicates equal loading of the resin. Retrieval of bait RNAs was monitored by urea PAGE and Syto60-staining of nucleic acids (lower panel). ( E, F ) Co-purification of the indicated miRNAs with the WT ZEB2-3′UTR or the MS2 control bait (MS2) from MCF7 cell lysates (E) or short let-7d-5p or miR-34a-5p antisense (as) bait transcripts from U2OS cell lysates (F) was monitored as described in (C). Error bars indicate s.d. of at least three independent analyses. Statistical significance was determined by Student’s t -test: * P
    Figure Legend Snippet: MiTRAP allows the selective co-purification of miRNAs with in vitro transcribed bait RNAs. ( A ) Scheme of the miTRAP procedure. In vitro transcribed bait RNAs comprising four MS2 stem-loops fused to the 3′end of bait transcripts were immobilized on amylose resin (light gray) via recombinant MBP-fused (dark gray) MS2-CP (white) protein. ( B ) Scheme of the used bait RNAs (upper panel). MS2: 120-nt-long control RNA encoded by the template vector; WT: wild type 3′UTR of the MYC mRNA; MUT: MYC-3′UTR with indicated point mutations (lower panel) in the overlapping MTS of the let-7-5p/miR-34-5p families. ( C ) Co-purification of miRNAs was determined by qRT-PCR and assessed by the miTRAP ratio that indicates the input normalized abundance of miRNAs in the miTRAP eluates (upper panel). MiTRAP ratios of indicated miRNAs were determined on co-purification with depicted RNA baits (B) from U2OS cell lysates. Ratios were determined by qRT-PCR using miRNA-specific TaqMan probes. Note that MYC-regulatory miRNAs of the let-7-5p and miR-34-5p families are selectively co-purified with the MYC-3′UTR bait RNA, but not the mutant 3′UTR or MS2 control transcript. MiTRAP ratios for the WT 3′UTR bait are two orders of magnitude (note logarithmic scale) above controls. Error bars indicate standard deviation (s.d.) of at least three independent analyses. Statistical significance was determined by Student’s t -test: * P ≤ 0.05. ( D ) Western blot analysis of indicated proteins isolated from U2OS input fractions or co-purified with MBP–MS2BP-coated amylose resin (BC), the MS2 control transcript (MS2), the WT or point mutated (MUT) MYC-3′UTR, respectively. TUBA4A served as negative control for unspecific binding, whereas MBP–MS2BP indicates equal loading of the resin. Retrieval of bait RNAs was monitored by urea PAGE and Syto60-staining of nucleic acids (lower panel). ( E, F ) Co-purification of the indicated miRNAs with the WT ZEB2-3′UTR or the MS2 control bait (MS2) from MCF7 cell lysates (E) or short let-7d-5p or miR-34a-5p antisense (as) bait transcripts from U2OS cell lysates (F) was monitored as described in (C). Error bars indicate s.d. of at least three independent analyses. Statistical significance was determined by Student’s t -test: * P

    Techniques Used: Copurification, In Vitro, Recombinant, Plasmid Preparation, Quantitative RT-PCR, Purification, Mutagenesis, Standard Deviation, Western Blot, Isolation, Negative Control, Binding Assay, Polyacrylamide Gel Electrophoresis, Staining

    16) Product Images from "Targeting CXCR7/ACKR3 as a therapeutic strategy to promote remyelination in the adult central nervous system"

    Article Title: Targeting CXCR7/ACKR3 as a therapeutic strategy to promote remyelination in the adult central nervous system

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20131224

    Chemokine expression and OPC population patterns throughout demyelination and remyelination. (A–E) Confocal IHC analysis of the CC of naive CXCR7 GFP/+ mice and after 3 or 6 wk of CPZ ingestion and after 10 d remyelination. Sections were stained for MBP (A), GFP (CXCR7; B), CXCR4 (C), CXCL12 (D), and NG2 (E). The mean positive area was analyzed for naive mice (N), mice fed CPZ for 3 or 6 wk, and mice fed CPZ for 6 wk and then a standard diet for a 10-d recovery period (R). (F and G) Confocal IHC analysis of the CC of CXCR7 GFP/+ mice after 3 wk (F) or 6 wk (G) of CPZ ingestion. Sections were stained for GFP (green) in combination with NG2, GFAP, Iba-1, CD31, and NFH (red). The area of co-localization was analyzed via Volocity image analysis software and normalized to the total GFP + area. Representative images are shown and quantitative data were collected from 3 sections per mouse ( n = 4–8 mice/group) across 2 independent experiments with results shown as fold change compared with naive ± SEM. Insets = isotype control (IC). Bars, 25 µm. *, P
    Figure Legend Snippet: Chemokine expression and OPC population patterns throughout demyelination and remyelination. (A–E) Confocal IHC analysis of the CC of naive CXCR7 GFP/+ mice and after 3 or 6 wk of CPZ ingestion and after 10 d remyelination. Sections were stained for MBP (A), GFP (CXCR7; B), CXCR4 (C), CXCL12 (D), and NG2 (E). The mean positive area was analyzed for naive mice (N), mice fed CPZ for 3 or 6 wk, and mice fed CPZ for 6 wk and then a standard diet for a 10-d recovery period (R). (F and G) Confocal IHC analysis of the CC of CXCR7 GFP/+ mice after 3 wk (F) or 6 wk (G) of CPZ ingestion. Sections were stained for GFP (green) in combination with NG2, GFAP, Iba-1, CD31, and NFH (red). The area of co-localization was analyzed via Volocity image analysis software and normalized to the total GFP + area. Representative images are shown and quantitative data were collected from 3 sections per mouse ( n = 4–8 mice/group) across 2 independent experiments with results shown as fold change compared with naive ± SEM. Insets = isotype control (IC). Bars, 25 µm. *, P

    Techniques Used: Expressing, Immunohistochemistry, Mouse Assay, Staining, Software

    17) Product Images from "Lesion stage-dependent causes for impaired remyelination in MS"

    Article Title: Lesion stage-dependent causes for impaired remyelination in MS

    Journal: Acta Neuropathologica

    doi: 10.1007/s00401-020-02189-9

    Supernatants of primary human M1, but not M2 or M0 polarized microglia inhibit the terminal differentiation of hiOL. Every experiment analyzing the effect of microglia supernatants on hiOL was performed with hiOL derived from three different donors. a – e To confirm successful polarization into M1 and M2 microglia, qRT-PCR was performed for IL-6, CXCL10 and TNFα (M1) and CD206 and CD209 (M2). f Differentiation of hiOL in the presence of supernatants from M0, M1 or M2 polarized microglia from one fetal donor (HFM2) from day 4 to 21 resulted in no significant differences in the percentage of O4 + hiOL when comparing the effect of different supernatants with appropriate controls. g , h Culturing O4 sorted hiOL in the presence of supernatants from M1, but not M0 or M2 polarized microglia from one fetal donor (HFM3) from day 21 to 35 impaired significantly the differentiation of O4 + hiOL into MBP + mature oligodendrocytes. i – k This was confirmed using supernatants from M1 and M0 polarized microglia from another fetal (HFM1) and one adult donor (HAM1) as well as supernatants from M0, M1 and M2 polarized microglia from a second adult donor (HAM2). l Analysis of percentages of actively dividing hiOL (Ki-67 + over O4 + cells) in the presence of supernatants from M0, M1 or M2 polarized microglia from one fetal donor (HFM2) from day 21 to 35 of differentiation did not reveal any significant differences. m Percentages of apoptotic hiOL (cleaved caspase 3 + over O4 + cells) in the presence of supernatants from M0, M1 or M2 polarized microglia from one fetal donor (HFM3) from day 21 to 35 of differentiation were not significantly altered. Scale bar in g : 100 µm. hiOL human iPSC-derived oligodendrocytes, HFM human fetal microglia, HAM human adult microglia, IL-6 interleukin 6, CXCL10 C-X-C motif chemokine 10, TNFα tumor necrosis factor alpha, CD206/CD209 cluster of differentiation 206/209, MBP myelin basic protein
    Figure Legend Snippet: Supernatants of primary human M1, but not M2 or M0 polarized microglia inhibit the terminal differentiation of hiOL. Every experiment analyzing the effect of microglia supernatants on hiOL was performed with hiOL derived from three different donors. a – e To confirm successful polarization into M1 and M2 microglia, qRT-PCR was performed for IL-6, CXCL10 and TNFα (M1) and CD206 and CD209 (M2). f Differentiation of hiOL in the presence of supernatants from M0, M1 or M2 polarized microglia from one fetal donor (HFM2) from day 4 to 21 resulted in no significant differences in the percentage of O4 + hiOL when comparing the effect of different supernatants with appropriate controls. g , h Culturing O4 sorted hiOL in the presence of supernatants from M1, but not M0 or M2 polarized microglia from one fetal donor (HFM3) from day 21 to 35 impaired significantly the differentiation of O4 + hiOL into MBP + mature oligodendrocytes. i – k This was confirmed using supernatants from M1 and M0 polarized microglia from another fetal (HFM1) and one adult donor (HAM1) as well as supernatants from M0, M1 and M2 polarized microglia from a second adult donor (HAM2). l Analysis of percentages of actively dividing hiOL (Ki-67 + over O4 + cells) in the presence of supernatants from M0, M1 or M2 polarized microglia from one fetal donor (HFM2) from day 21 to 35 of differentiation did not reveal any significant differences. m Percentages of apoptotic hiOL (cleaved caspase 3 + over O4 + cells) in the presence of supernatants from M0, M1 or M2 polarized microglia from one fetal donor (HFM3) from day 21 to 35 of differentiation were not significantly altered. Scale bar in g : 100 µm. hiOL human iPSC-derived oligodendrocytes, HFM human fetal microglia, HAM human adult microglia, IL-6 interleukin 6, CXCL10 C-X-C motif chemokine 10, TNFα tumor necrosis factor alpha, CD206/CD209 cluster of differentiation 206/209, MBP myelin basic protein

    Techniques Used: Derivative Assay, Quantitative RT-PCR

    18) Product Images from "Postmortem and imaging based analyses reveal CNS decreased myelination in restless legs syndrome"

    Article Title: Postmortem and imaging based analyses reveal CNS decreased myelination in restless legs syndrome

    Journal: Sleep medicine

    doi: 10.1016/j.sleep.2010.10.009

    Western blot analyses of myelin-related proteins in RLS and control brain autopsies. MBP (1a), PLP (1b) and CNPase (1c) are significantly decreased in RLS. Solid line represents the median value and the error bars represent the SEM (*p
    Figure Legend Snippet: Western blot analyses of myelin-related proteins in RLS and control brain autopsies. MBP (1a), PLP (1b) and CNPase (1c) are significantly decreased in RLS. Solid line represents the median value and the error bars represent the SEM (*p

    Techniques Used: Western Blot, Plasmid Purification

    19) Product Images from "Collagen-Binding Hepatocyte Growth Factor (HGF) alone or with a Gelatin- furfurylamine Hydrogel Enhances Functional Recovery in Mice after Spinal Cord Injury"

    Article Title: Collagen-Binding Hepatocyte Growth Factor (HGF) alone or with a Gelatin- furfurylamine Hydrogel Enhances Functional Recovery in Mice after Spinal Cord Injury

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-19316-y

    Axonal growth after spinal cord transection injury. Mice subjected to a transection injury were treated with gelatin-FA hydrogel alone, gelatin-FA hydrogel + CBD-HGF or gelatin-FA hydrogel + HGF. Untreated mice were used as controls (5 mice each per 4 groups). Immunohistochemistry for GAP43 ( a ), MBP ( b ) and GFAP ( c ) at the injury sites was performed 8 weeks post-injury. Left: Representative photographs from each group. The scale bar indicates 500 µm (dotted line) and 50 µm (solid line). Right: Immunopositive areas were measured under a microscope and percent area is shown. * p
    Figure Legend Snippet: Axonal growth after spinal cord transection injury. Mice subjected to a transection injury were treated with gelatin-FA hydrogel alone, gelatin-FA hydrogel + CBD-HGF or gelatin-FA hydrogel + HGF. Untreated mice were used as controls (5 mice each per 4 groups). Immunohistochemistry for GAP43 ( a ), MBP ( b ) and GFAP ( c ) at the injury sites was performed 8 weeks post-injury. Left: Representative photographs from each group. The scale bar indicates 500 µm (dotted line) and 50 µm (solid line). Right: Immunopositive areas were measured under a microscope and percent area is shown. * p

    Techniques Used: Mouse Assay, Immunohistochemistry, Microscopy

    Axonal growth after spinal cord compression injury. Mice subjected to a compression injury were treated with a single administration of CBD-HGF or HGF. Untreated mice were used as controls (4 mice each per 3 groups). Immunohistochemistry for GAP43 ( a ), MBP ( b ) and GFAP ( c ) at the injury sites was performed 6 weeks post-injury. Left: Representative photographs from each group. The scale bar indicates 500 µm (solid line) and 50 µm (dotted line). Right: Immunopositive areas were measured under a microscope and percent area is shown. * p
    Figure Legend Snippet: Axonal growth after spinal cord compression injury. Mice subjected to a compression injury were treated with a single administration of CBD-HGF or HGF. Untreated mice were used as controls (4 mice each per 3 groups). Immunohistochemistry for GAP43 ( a ), MBP ( b ) and GFAP ( c ) at the injury sites was performed 6 weeks post-injury. Left: Representative photographs from each group. The scale bar indicates 500 µm (solid line) and 50 µm (dotted line). Right: Immunopositive areas were measured under a microscope and percent area is shown. * p

    Techniques Used: Mouse Assay, Immunohistochemistry, Microscopy

    20) Product Images from "Effects of Erythropoietin on Gliogenesis during Cerebral Ischemic/Reperfusion Recovery in Adult Mice"

    Article Title: Effects of Erythropoietin on Gliogenesis during Cerebral Ischemic/Reperfusion Recovery in Adult Mice

    Journal: Aging and Disease

    doi: 10.14336/AD.2016.1209

    EPO attenuates white matter injury after MCAO MBP (red) and NF-200 (green) immunostaining 14 days after I/R ( A-C ). Boxes in ( A ) indicate the selected areas of the cortex (CTX), corpus callosum (CC), and striatum (ST). Scale bar, 1 mm. Immunostaining of MBP and NF-200 in the CTX, CC, and ST in the ipsilateral hemisphere ( B ). Scale bar, 50 μm. Quantification of the relative ratio of NF-200 vs. MBP immunostaining intensity in ipsilateral hemispheres ( C ). Relative expression levels of myelin proteins (CNPase and MBP) in the ipsilateral brain tissue of different groups ( D ). β-actin served as a loading control. n=4 per group. Representative western blots for results in D ( E-F ). * P ≤0.05, ** P ≤0.01 vs. sham; # P ≤0.05 vs. I/R vehicle. n=4 per group.
    Figure Legend Snippet: EPO attenuates white matter injury after MCAO MBP (red) and NF-200 (green) immunostaining 14 days after I/R ( A-C ). Boxes in ( A ) indicate the selected areas of the cortex (CTX), corpus callosum (CC), and striatum (ST). Scale bar, 1 mm. Immunostaining of MBP and NF-200 in the CTX, CC, and ST in the ipsilateral hemisphere ( B ). Scale bar, 50 μm. Quantification of the relative ratio of NF-200 vs. MBP immunostaining intensity in ipsilateral hemispheres ( C ). Relative expression levels of myelin proteins (CNPase and MBP) in the ipsilateral brain tissue of different groups ( D ). β-actin served as a loading control. n=4 per group. Representative western blots for results in D ( E-F ). * P ≤0.05, ** P ≤0.01 vs. sham; # P ≤0.05 vs. I/R vehicle. n=4 per group.

    Techniques Used: Immunostaining, Expressing, Western Blot

    21) Product Images from "Functional Genomic Analysis of Oligodendrocyte Differentiation"

    Article Title: Functional Genomic Analysis of Oligodendrocyte Differentiation

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.2572-06.2006

    Temporal expression patterns of regulated myelin and cell cycle control/DNA replication genes. A , B as supplemental material). A , Genes induced immediately after PDGF/NT3 withdrawal and T3 exposure are highlighted in color ( OSP , MBP , UGT8 , CNP1 , PLP ), with late-induced myelin-related genes shown in gray. B , Genes whose induction is delayed after PDGF/NT3 withdrawal and T3 exposure are highlighted in color ( Tm4sf11 , Tspan2 , MOG , MAG , ASPA , MOBP , MAL ). C–E as supplemental material). C , Genes repressed soon after PDGF/NT3 withdrawal and T3 exposure are highlighted in color, with remaining genes shown in gray. D , Genes that immediately peak and are subsequently downregulated after PDGF/NT3 withdrawal and T3 exposure are highlighted in color. E , Genes induced after PDGF/NT3 withdrawal and T3 exposure are highlighted in color. In all graphs, expression levels are determined as average fold changes relative to average OPC levels and expressed on a log 2 scale at the time points indicated. AcOL, Acutely purified OL sample.
    Figure Legend Snippet: Temporal expression patterns of regulated myelin and cell cycle control/DNA replication genes. A , B as supplemental material). A , Genes induced immediately after PDGF/NT3 withdrawal and T3 exposure are highlighted in color ( OSP , MBP , UGT8 , CNP1 , PLP ), with late-induced myelin-related genes shown in gray. B , Genes whose induction is delayed after PDGF/NT3 withdrawal and T3 exposure are highlighted in color ( Tm4sf11 , Tspan2 , MOG , MAG , ASPA , MOBP , MAL ). C–E as supplemental material). C , Genes repressed soon after PDGF/NT3 withdrawal and T3 exposure are highlighted in color, with remaining genes shown in gray. D , Genes that immediately peak and are subsequently downregulated after PDGF/NT3 withdrawal and T3 exposure are highlighted in color. E , Genes induced after PDGF/NT3 withdrawal and T3 exposure are highlighted in color. In all graphs, expression levels are determined as average fold changes relative to average OPC levels and expressed on a log 2 scale at the time points indicated. AcOL, Acutely purified OL sample.

    Techniques Used: Expressing, Plasmid Purification, Purification

    22) Product Images from "Cellular Sources and Regional Variations in the Expression of the Neuroinflammatory Marker Translocator Protein (TSPO) in the Normal Brain"

    Article Title: Cellular Sources and Regional Variations in the Expression of the Neuroinflammatory Marker Translocator Protein (TSPO) in the Normal Brain

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms19092707

    TSPO expression across the olfactory bulb in the normal mouse brain. ( A ) Sagittal view of TSPO expression (red) across the olfactory bulb. Structural features of the region highlighted with DAPI (blue). ( B ) Concentrated TSPO expression (red) is observed in the subependymal zone, which houses a neural stem cell niche derived from the rostral migratory stream (RMS). TSPO colocalizes at low levels with GFAP+ (green) cells. ( C ) TSPO is present at the base of the RMS, entering into the olfactory bulb. Here, TSPO strongly colocalizes with Nestin+ (green) cells. ( D ) TSPO expression (red) is also observed in the olfactory nerve layer and glomerular layer, though does not colocalize with MBP+ oligodendrocytes (green) of the region. Scale bars = 300 µm for ( A ), 20 µm for ( B – D ). RMS = rostral migratory stream, SEZ = subependymal zone, OFT = olfactory fibre tracts, GL = glomerular layer.
    Figure Legend Snippet: TSPO expression across the olfactory bulb in the normal mouse brain. ( A ) Sagittal view of TSPO expression (red) across the olfactory bulb. Structural features of the region highlighted with DAPI (blue). ( B ) Concentrated TSPO expression (red) is observed in the subependymal zone, which houses a neural stem cell niche derived from the rostral migratory stream (RMS). TSPO colocalizes at low levels with GFAP+ (green) cells. ( C ) TSPO is present at the base of the RMS, entering into the olfactory bulb. Here, TSPO strongly colocalizes with Nestin+ (green) cells. ( D ) TSPO expression (red) is also observed in the olfactory nerve layer and glomerular layer, though does not colocalize with MBP+ oligodendrocytes (green) of the region. Scale bars = 300 µm for ( A ), 20 µm for ( B – D ). RMS = rostral migratory stream, SEZ = subependymal zone, OFT = olfactory fibre tracts, GL = glomerular layer.

    Techniques Used: Expressing, Derivative Assay

    23) Product Images from "Phospholipase A2 is involved in galactosylsphingosine-induced astrocyte toxicity, neuronal damage and demyelination"

    Article Title: Phospholipase A2 is involved in galactosylsphingosine-induced astrocyte toxicity, neuronal damage and demyelination

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0187217

    DEDA treatment inhibits psychosine-induced demyelination of cerebellar slices. (A) Bar graph illustrating changes in MBP, MOG and NFH staining after psychosine (100 nM) ± DEDA (100 nM) treatments. (B) Representative confocal images displaying MBP (MBP, green), MOG (MOG, yellow) and neurofilament (NFH, red) immunostaining under treatment conditions indicated. Confocal images captured at ×10 magnification; scale bar 200μm. Mean fluorescence was calculated using a total of 25–36 independent ROI observations in each experiment. Data are presented as mean±s.e.m. (n = 7), one-way ANOVA and Newman–Keuls multiple comparison post-test *p
    Figure Legend Snippet: DEDA treatment inhibits psychosine-induced demyelination of cerebellar slices. (A) Bar graph illustrating changes in MBP, MOG and NFH staining after psychosine (100 nM) ± DEDA (100 nM) treatments. (B) Representative confocal images displaying MBP (MBP, green), MOG (MOG, yellow) and neurofilament (NFH, red) immunostaining under treatment conditions indicated. Confocal images captured at ×10 magnification; scale bar 200μm. Mean fluorescence was calculated using a total of 25–36 independent ROI observations in each experiment. Data are presented as mean±s.e.m. (n = 7), one-way ANOVA and Newman–Keuls multiple comparison post-test *p

    Techniques Used: Staining, Immunostaining, Fluorescence

    24) Product Images from "Myelin injury induces axonal transport impairment but not AD-like pathology in the hippocampus of cuprizone-fed mice"

    Article Title: Myelin injury induces axonal transport impairment but not AD-like pathology in the hippocampus of cuprizone-fed mice

    Journal: Oncotarget

    doi: 10.18632/oncotarget.8981

    Demyelination in the hippocampus Myelin associated proteins in the hippocampus, including MBP A. , CNPase B. and PLP C. , were detected among the three groups using Western-blot. Figures D. - L. were displayed the immunofluorescence staining of MBP in subregions of the hippocampus, including Ca3 (D-F), Ca1 (G-I) and DG (J-L). *denotes statistical significance compared with controls ( P
    Figure Legend Snippet: Demyelination in the hippocampus Myelin associated proteins in the hippocampus, including MBP A. , CNPase B. and PLP C. , were detected among the three groups using Western-blot. Figures D. - L. were displayed the immunofluorescence staining of MBP in subregions of the hippocampus, including Ca3 (D-F), Ca1 (G-I) and DG (J-L). *denotes statistical significance compared with controls ( P

    Techniques Used: Plasmid Purification, Western Blot, Immunofluorescence, Staining

    25) Product Images from "A 3D disease and regeneration model of peripheral nervous system–on–a–chip"

    Article Title: A 3D disease and regeneration model of peripheral nervous system–on–a–chip

    Journal: Science Advances

    doi: 10.1126/sciadv.abd9749

    Reconstruction of 3D demyelination in SC-MN coculture by LPC treatment. ( A ) Schematic diagram displaying the time course of LPC introduction and demyelination analysis in the SC-MN myelination model. To induce demyelination, cocultures were treated with 0.35 or 0.7 mM LPC, and the demyelination of SC-MN cocultures was measured by immunocytochemistry at DIV 17, 20, and 23. Fixed samples were immunostained with antibodies against c-Jun (gray), myelin basic protein (MBP; green), tubulin beta III (TuJ1; red), and 4′,6-diamidino-2-phenylindole (DAPI) (blue). ( B ) Schematic illustrations displaying the SC-MN coculture from myelination to demyelination. ( C ) Representative bright-field image for establishment of a demyelinating coculture system. Demyelination was rapidly induced by LPC treatment, and uneven nerve fiber (yellow arrowheads) was observed in the gel channel at DIV 20. ( D ) Representative confocal images of cocultures and quantification of c-Jun + cells ( E ) and MBP intensity ( F ) with or without 0.35 or 0.7 mM LPC treatment, respectively. The graph shows mean ± SEM values from at least three independent experiments using different mice. * P
    Figure Legend Snippet: Reconstruction of 3D demyelination in SC-MN coculture by LPC treatment. ( A ) Schematic diagram displaying the time course of LPC introduction and demyelination analysis in the SC-MN myelination model. To induce demyelination, cocultures were treated with 0.35 or 0.7 mM LPC, and the demyelination of SC-MN cocultures was measured by immunocytochemistry at DIV 17, 20, and 23. Fixed samples were immunostained with antibodies against c-Jun (gray), myelin basic protein (MBP; green), tubulin beta III (TuJ1; red), and 4′,6-diamidino-2-phenylindole (DAPI) (blue). ( B ) Schematic illustrations displaying the SC-MN coculture from myelination to demyelination. ( C ) Representative bright-field image for establishment of a demyelinating coculture system. Demyelination was rapidly induced by LPC treatment, and uneven nerve fiber (yellow arrowheads) was observed in the gel channel at DIV 20. ( D ) Representative confocal images of cocultures and quantification of c-Jun + cells ( E ) and MBP intensity ( F ) with or without 0.35 or 0.7 mM LPC treatment, respectively. The graph shows mean ± SEM values from at least three independent experiments using different mice. * P

    Techniques Used: Immunocytochemistry, Mouse Assay

    Reconstruction of 3D remyelination by either Benz or MeCbl treatment on demyelinated SC-MN coculture. ( A ) To verify remyelination, cocultures were cotreated with Benz (1.5 μM) or MeCbl (10 μM) in the presence of LPC at DIV 20 and analyzed at DIV 28. ( B ) Schematic illustrations displaying the coculture from demyelination to remyelination. ( C ) Representative bright-field image for establishment of a remyelinating coculture system. ( D ) Representative confocal images of cocultures with MBP (green), TuJ1 (red), and DAPI (blue); ( E ) quantification of MBP intensity and ( F ) immunoblots; and ( G ) quantification of MBP on cocultures with Benz or MeCbl in the presence and absence of LPC are shown. ( H ) Representative inverted confocal images of BAPTA-1 AM signals showing the time points of the baseline ( F 0 ) and peak (∆ F ) levels, ( I ) cell recording, and ( J ) quantification of the relative changes in intracellular Ca 2+ in cocultures with LPC only and with LPC plus Benz or MeCbl treatment. In (F) and (J), the graph shows mean ± SEM values from three independent experiments using different mice. In (J), an average of 13 cells per independent experiment was calculated. * P
    Figure Legend Snippet: Reconstruction of 3D remyelination by either Benz or MeCbl treatment on demyelinated SC-MN coculture. ( A ) To verify remyelination, cocultures were cotreated with Benz (1.5 μM) or MeCbl (10 μM) in the presence of LPC at DIV 20 and analyzed at DIV 28. ( B ) Schematic illustrations displaying the coculture from demyelination to remyelination. ( C ) Representative bright-field image for establishment of a remyelinating coculture system. ( D ) Representative confocal images of cocultures with MBP (green), TuJ1 (red), and DAPI (blue); ( E ) quantification of MBP intensity and ( F ) immunoblots; and ( G ) quantification of MBP on cocultures with Benz or MeCbl in the presence and absence of LPC are shown. ( H ) Representative inverted confocal images of BAPTA-1 AM signals showing the time points of the baseline ( F 0 ) and peak (∆ F ) levels, ( I ) cell recording, and ( J ) quantification of the relative changes in intracellular Ca 2+ in cocultures with LPC only and with LPC plus Benz or MeCbl treatment. In (F) and (J), the graph shows mean ± SEM values from three independent experiments using different mice. In (J), an average of 13 cells per independent experiment was calculated. * P

    Techniques Used: Western Blot, Mouse Assay

    26) Product Images from "Lactosylceramide synthases encoded by B4galt5 and 6 genes are pivotal for neuronal generation and myelin formation in mice"

    Article Title: Lactosylceramide synthases encoded by B4galt5 and 6 genes are pivotal for neuronal generation and myelin formation in mice

    Journal: PLoS Genetics

    doi: 10.1371/journal.pgen.1007545

    Immunohistochemical analysis of myelin-associated proteins and neurofilament (NF) in the cerebral cortex. (A–D) Immunohistochemical staining for MBP (A, red), MAG (B, red), MOG (C, red), and PLP (D, red) merged with NF (green) and DAPI (blue) in the cerebral cortices of wild-type (wt) and double knockout (DKO) mice at 3 weeks of age.
    Figure Legend Snippet: Immunohistochemical analysis of myelin-associated proteins and neurofilament (NF) in the cerebral cortex. (A–D) Immunohistochemical staining for MBP (A, red), MAG (B, red), MOG (C, red), and PLP (D, red) merged with NF (green) and DAPI (blue) in the cerebral cortices of wild-type (wt) and double knockout (DKO) mice at 3 weeks of age.

    Techniques Used: Immunohistochemistry, Staining, Plasmid Purification, Double Knockout, Mouse Assay

    Immunohistochemical and TEM analysis in the spinal cord. (A) Immunohistochemical staining for MBP, MAG, MOG, and PLP (red) merged with NF (green) in the spinal cords of wild-type (wt) and double knockout (DKO) mice at 3 weeks of age. (B) TEM analysis of the spinal cords of wt (upper) and DKO (lower) mice at 3 weeks of age. Right-hand pictures show a higher magnification of the squares in the left-hand pictures. Arrowheads: small axons without a myelin sheath; arrows: large axons with low circularity. (C) Frequency of axon circularity in wt (n = 1930) and DKO (n = 2120) mice. (D) The g-ratio (axon diameter/myelinated fiber diameter) of large axons of wt (n = 130) and DKO mice (n = 95) was measured. **, p
    Figure Legend Snippet: Immunohistochemical and TEM analysis in the spinal cord. (A) Immunohistochemical staining for MBP, MAG, MOG, and PLP (red) merged with NF (green) in the spinal cords of wild-type (wt) and double knockout (DKO) mice at 3 weeks of age. (B) TEM analysis of the spinal cords of wt (upper) and DKO (lower) mice at 3 weeks of age. Right-hand pictures show a higher magnification of the squares in the left-hand pictures. Arrowheads: small axons without a myelin sheath; arrows: large axons with low circularity. (C) Frequency of axon circularity in wt (n = 1930) and DKO (n = 2120) mice. (D) The g-ratio (axon diameter/myelinated fiber diameter) of large axons of wt (n = 130) and DKO mice (n = 95) was measured. **, p

    Techniques Used: Immunohistochemistry, Transmission Electron Microscopy, Staining, Plasmid Purification, Double Knockout, Mouse Assay

    27) Product Images from "AATYK is a Novel Regulator of Oligodendrocyte Differentiation and Myelination"

    Article Title: AATYK is a Novel Regulator of Oligodendrocyte Differentiation and Myelination

    Journal: Neuroscience Bulletin

    doi: 10.1007/s12264-018-0218-6

    AATYK knockdown does not affect the survival but maintains proliferation of OPCs. A Ki67 immunostaining showed there were more Ki67 + and GFP + co-labeled cells in the sh- AATYK -treated OPCs than in the control. B Percentages of Ki67 + and GFP + co-labelled OLs in GFP + cells among RNAi- AATYK cells were significantly higher than in the control. C – D Compared to control cells, the expression of MBP protein was significantly decreased in shRNA- AATYK -treated cells and there was no difference in caspase3 between the two groups. Scale bar, 100 μm.
    Figure Legend Snippet: AATYK knockdown does not affect the survival but maintains proliferation of OPCs. A Ki67 immunostaining showed there were more Ki67 + and GFP + co-labeled cells in the sh- AATYK -treated OPCs than in the control. B Percentages of Ki67 + and GFP + co-labelled OLs in GFP + cells among RNAi- AATYK cells were significantly higher than in the control. C – D Compared to control cells, the expression of MBP protein was significantly decreased in shRNA- AATYK -treated cells and there was no difference in caspase3 between the two groups. Scale bar, 100 μm.

    Techniques Used: Immunostaining, Labeling, Expressing, shRNA

    28) Product Images from "An integrated multi-layer 3D-fabrication of PDA/RGD coated graphene loaded PCL nanoscaffold for peripheral nerve restoration"

    Article Title: An integrated multi-layer 3D-fabrication of PDA/RGD coated graphene loaded PCL nanoscaffold for peripheral nerve restoration

    Journal: Nature Communications

    doi: 10.1038/s41467-017-02598-7

    Triple immunofluorescent staining of S100 and MBP at 18 weeks post operatively. S100 (green), MBP (red), and nuclei (blue) were exhibited from different groups respectively. a – d SC-loaded PDA/RGD-SG/PCL. e – h SC-loaded PDA/RGD-MG/PCL. i – l PDA/RGD-SG/PCL. m – p PDA/RGD-MG/PCL. q – t PDA/RGD-PCL. u – x Autograft. The scale bar is 100 μm
    Figure Legend Snippet: Triple immunofluorescent staining of S100 and MBP at 18 weeks post operatively. S100 (green), MBP (red), and nuclei (blue) were exhibited from different groups respectively. a – d SC-loaded PDA/RGD-SG/PCL. e – h SC-loaded PDA/RGD-MG/PCL. i – l PDA/RGD-SG/PCL. m – p PDA/RGD-MG/PCL. q – t PDA/RGD-PCL. u – x Autograft. The scale bar is 100 μm

    Techniques Used: Staining

    29) Product Images from "Lactosylceramide synthases encoded by B4galt5 and 6 genes are pivotal for neuronal generation and myelin formation in mice"

    Article Title: Lactosylceramide synthases encoded by B4galt5 and 6 genes are pivotal for neuronal generation and myelin formation in mice

    Journal: PLoS Genetics

    doi: 10.1371/journal.pgen.1007545

    Immunohistochemical analysis of myelin-associated proteins and neurofilament (NF) in the cerebral cortex. (A–D) Immunohistochemical staining for MBP (A, red), MAG (B, red), MOG (C, red), and PLP (D, red) merged with NF (green) and DAPI (blue) in the cerebral cortices of wild-type (wt) and double knockout (DKO) mice at 3 weeks of age.
    Figure Legend Snippet: Immunohistochemical analysis of myelin-associated proteins and neurofilament (NF) in the cerebral cortex. (A–D) Immunohistochemical staining for MBP (A, red), MAG (B, red), MOG (C, red), and PLP (D, red) merged with NF (green) and DAPI (blue) in the cerebral cortices of wild-type (wt) and double knockout (DKO) mice at 3 weeks of age.

    Techniques Used: Immunohistochemistry, Staining, Plasmid Purification, Double Knockout, Mouse Assay

    Immunohistochemical and TEM analysis in the spinal cord. (A) Immunohistochemical staining for MBP, MAG, MOG, and PLP (red) merged with NF (green) in the spinal cords of wild-type (wt) and double knockout (DKO) mice at 3 weeks of age. (B) TEM analysis of the spinal cords of wt (upper) and DKO (lower) mice at 3 weeks of age. Right-hand pictures show a higher magnification of the squares in the left-hand pictures. Arrowheads: small axons without a myelin sheath; arrows: large axons with low circularity. (C) Frequency of axon circularity in wt (n = 1930) and DKO (n = 2120) mice. (D) The g-ratio (axon diameter/myelinated fiber diameter) of large axons of wt (n = 130) and DKO mice (n = 95) was measured. **, p
    Figure Legend Snippet: Immunohistochemical and TEM analysis in the spinal cord. (A) Immunohistochemical staining for MBP, MAG, MOG, and PLP (red) merged with NF (green) in the spinal cords of wild-type (wt) and double knockout (DKO) mice at 3 weeks of age. (B) TEM analysis of the spinal cords of wt (upper) and DKO (lower) mice at 3 weeks of age. Right-hand pictures show a higher magnification of the squares in the left-hand pictures. Arrowheads: small axons without a myelin sheath; arrows: large axons with low circularity. (C) Frequency of axon circularity in wt (n = 1930) and DKO (n = 2120) mice. (D) The g-ratio (axon diameter/myelinated fiber diameter) of large axons of wt (n = 130) and DKO mice (n = 95) was measured. **, p

    Techniques Used: Immunohistochemistry, Transmission Electron Microscopy, Staining, Plasmid Purification, Double Knockout, Mouse Assay

    30) Product Images from "Tomato Yellow Leaf Curl Virus V2 Interacts with Host Histone Deacetylase 6 To Suppress Methylation-Mediated Transcriptional Gene Silencing in Plants"

    Article Title: Tomato Yellow Leaf Curl Virus V2 Interacts with Host Histone Deacetylase 6 To Suppress Methylation-Mediated Transcriptional Gene Silencing in Plants

    Journal: Journal of Virology

    doi: 10.1128/JVI.00036-18

    V2 competes with NbMET1 for direct binding to NbHDA6. (A) In vitro competitive pulldown assays. The indicated amounts of V2-HIS or HIS protein were mixed with 2 μg of MBP-NbMET1 and pulled down by 2 μg of GST-NbHDA6. The bound protein was detected by immunoblotting with the indicated antibodies. (B) In vivo competitive co-IP assays. NbHDA6-FLAG and NbMET1-GFP were coexpressed in Arabidopsis protoplasts in the presence or absence of V2-HA. The immune complexes were pulled down by using anti-FLAG agarose beads. These experiments were repeated three times with similar results.
    Figure Legend Snippet: V2 competes with NbMET1 for direct binding to NbHDA6. (A) In vitro competitive pulldown assays. The indicated amounts of V2-HIS or HIS protein were mixed with 2 μg of MBP-NbMET1 and pulled down by 2 μg of GST-NbHDA6. The bound protein was detected by immunoblotting with the indicated antibodies. (B) In vivo competitive co-IP assays. NbHDA6-FLAG and NbMET1-GFP were coexpressed in Arabidopsis protoplasts in the presence or absence of V2-HA. The immune complexes were pulled down by using anti-FLAG agarose beads. These experiments were repeated three times with similar results.

    Techniques Used: Binding Assay, In Vitro, In Vivo, Co-Immunoprecipitation Assay

    31) Product Images from "Microglia knockdown reduces inflammation and preserves cognition in diabetic animals after experimental stroke"

    Article Title: Microglia knockdown reduces inflammation and preserves cognition in diabetic animals after experimental stroke

    Journal: Journal of Neuroinflammation

    doi: 10.1186/s12974-020-01815-3

    CSF1R KD improved myelination. a – c Depicts representative images of GFAP+ cells of slice B for sham, NTC, CSF1R KD animals, respectively. d There was no significant difference in the number of GFAP+ cells in the PFC or limbic structures of slice B and GFAP+ cells. e Depicts representative images of NF200+ and MBP+ cells of slice B for NTC, CSF1R KD, and sham animals, respectively. Using MBP to stain myelin and NF200 to stain the axons, we evaluated the ratio of MBP:NF200 as a measure of myelination ( e ). Although diabetic animals exhibited demyelination 3 weeks after stroke, CSF1R KD prevented this decline in myelination ( N = 5–7/group)
    Figure Legend Snippet: CSF1R KD improved myelination. a – c Depicts representative images of GFAP+ cells of slice B for sham, NTC, CSF1R KD animals, respectively. d There was no significant difference in the number of GFAP+ cells in the PFC or limbic structures of slice B and GFAP+ cells. e Depicts representative images of NF200+ and MBP+ cells of slice B for NTC, CSF1R KD, and sham animals, respectively. Using MBP to stain myelin and NF200 to stain the axons, we evaluated the ratio of MBP:NF200 as a measure of myelination ( e ). Although diabetic animals exhibited demyelination 3 weeks after stroke, CSF1R KD prevented this decline in myelination ( N = 5–7/group)

    Techniques Used: Staining

    32) Product Images from "Lactosylceramide synthases encoded by B4galt5 and 6 genes are pivotal for neuronal generation and myelin formation in mice"

    Article Title: Lactosylceramide synthases encoded by B4galt5 and 6 genes are pivotal for neuronal generation and myelin formation in mice

    Journal: PLoS Genetics

    doi: 10.1371/journal.pgen.1007545

    Immunohistochemical analysis of myelin-associated proteins and neurofilament (NF) in the cerebral cortex. (A–D) Immunohistochemical staining for MBP (A, red), MAG (B, red), MOG (C, red), and PLP (D, red) merged with NF (green) and DAPI (blue) in the cerebral cortices of wild-type (wt) and double knockout (DKO) mice at 3 weeks of age.
    Figure Legend Snippet: Immunohistochemical analysis of myelin-associated proteins and neurofilament (NF) in the cerebral cortex. (A–D) Immunohistochemical staining for MBP (A, red), MAG (B, red), MOG (C, red), and PLP (D, red) merged with NF (green) and DAPI (blue) in the cerebral cortices of wild-type (wt) and double knockout (DKO) mice at 3 weeks of age.

    Techniques Used: Immunohistochemistry, Staining, Plasmid Purification, Double Knockout, Mouse Assay

    Immunohistochemical and TEM analysis in the spinal cord. (A) Immunohistochemical staining for MBP, MAG, MOG, and PLP (red) merged with NF (green) in the spinal cords of wild-type (wt) and double knockout (DKO) mice at 3 weeks of age. (B) TEM analysis of the spinal cords of wt (upper) and DKO (lower) mice at 3 weeks of age. Right-hand pictures show a higher magnification of the squares in the left-hand pictures. Arrowheads: small axons without a myelin sheath; arrows: large axons with low circularity. (C) Frequency of axon circularity in wt (n = 1930) and DKO (n = 2120) mice. (D) The g-ratio (axon diameter/myelinated fiber diameter) of large axons of wt (n = 130) and DKO mice (n = 95) was measured. **, p
    Figure Legend Snippet: Immunohistochemical and TEM analysis in the spinal cord. (A) Immunohistochemical staining for MBP, MAG, MOG, and PLP (red) merged with NF (green) in the spinal cords of wild-type (wt) and double knockout (DKO) mice at 3 weeks of age. (B) TEM analysis of the spinal cords of wt (upper) and DKO (lower) mice at 3 weeks of age. Right-hand pictures show a higher magnification of the squares in the left-hand pictures. Arrowheads: small axons without a myelin sheath; arrows: large axons with low circularity. (C) Frequency of axon circularity in wt (n = 1930) and DKO (n = 2120) mice. (D) The g-ratio (axon diameter/myelinated fiber diameter) of large axons of wt (n = 130) and DKO mice (n = 95) was measured. **, p

    Techniques Used: Immunohistochemistry, Transmission Electron Microscopy, Staining, Plasmid Purification, Double Knockout, Mouse Assay

    Related Articles

    Purification:

    Article Title: Overexpression of human BAG3P209L in mice causes restrictive cardiomyopathy
    Article Snippet: .. The following antibodies were used for protein detection: anti-BAG3 (10599-1-AP, Proteintech Group, 1:1000), anti-His (MCA1396, Serotec, 1:1000), anti-HSPA8 (raised against purified rat HSPA8 following recombinant expression in insect cells, rabbit immunization and antibody purification were performed at BioGenes GmbH, 1:1000), anti-HSPB8 (#3059, Cell Signaling Technology, 1:1000) and anti-MBP (ab23903, Abcam, 1:1000). ..

    Recombinant:

    Article Title: Overexpression of human BAG3P209L in mice causes restrictive cardiomyopathy
    Article Snippet: .. The following antibodies were used for protein detection: anti-BAG3 (10599-1-AP, Proteintech Group, 1:1000), anti-His (MCA1396, Serotec, 1:1000), anti-HSPA8 (raised against purified rat HSPA8 following recombinant expression in insect cells, rabbit immunization and antibody purification were performed at BioGenes GmbH, 1:1000), anti-HSPB8 (#3059, Cell Signaling Technology, 1:1000) and anti-MBP (ab23903, Abcam, 1:1000). ..

    Expressing:

    Article Title: Overexpression of human BAG3P209L in mice causes restrictive cardiomyopathy
    Article Snippet: .. The following antibodies were used for protein detection: anti-BAG3 (10599-1-AP, Proteintech Group, 1:1000), anti-His (MCA1396, Serotec, 1:1000), anti-HSPA8 (raised against purified rat HSPA8 following recombinant expression in insect cells, rabbit immunization and antibody purification were performed at BioGenes GmbH, 1:1000), anti-HSPB8 (#3059, Cell Signaling Technology, 1:1000) and anti-MBP (ab23903, Abcam, 1:1000). ..

    Antibody Purification:

    Article Title: Overexpression of human BAG3P209L in mice causes restrictive cardiomyopathy
    Article Snippet: .. The following antibodies were used for protein detection: anti-BAG3 (10599-1-AP, Proteintech Group, 1:1000), anti-His (MCA1396, Serotec, 1:1000), anti-HSPA8 (raised against purified rat HSPA8 following recombinant expression in insect cells, rabbit immunization and antibody purification were performed at BioGenes GmbH, 1:1000), anti-HSPB8 (#3059, Cell Signaling Technology, 1:1000) and anti-MBP (ab23903, Abcam, 1:1000). ..

    Staining:

    Article Title: A novel cyclic peptide (Naturido) modulates glia–neuron interactions in vitro and reverses ageing-related deficits in senescence-accelerated mice
    Article Snippet: .. Subsequently, for the identification of nuclei and cell types, DAPI for nuclear staining and primary antibodies (anti-MAP2 [a neuronal marker] produced in chickens, anti-EAAT2 [an astrocyte marker] produced in sheep, anti-GFAP [anther astrocyte marker] produced in chicken, anti- CD11b/c [a microglial marker] produced in mice, and anti-MBP [an oligodendrocyte marker] produced in mice; all manufactured by Abcam) were added to the cells after blocking, followed by shaking for 1 h at room temperature. ..

    Article Title: Therapeutic Effect of Ginsenoside Rd on Experimental Autoimmune Encephalomyelitis Model Mice: Regulation of Inflammation and Treg/Th17 Cell Balance
    Article Snippet: .. The spinal cord sections in each group were firstly incubated in blocking solution (3% donkey serum, 0.3% Triton X-100 in PBS) for 30 min and stained with primary antibodies anti-MBP (dilution ratio, 1 : 200; Abcam, UK) at 4°C overnight. ..

    Marker:

    Article Title: A novel cyclic peptide (Naturido) modulates glia–neuron interactions in vitro and reverses ageing-related deficits in senescence-accelerated mice
    Article Snippet: .. Subsequently, for the identification of nuclei and cell types, DAPI for nuclear staining and primary antibodies (anti-MAP2 [a neuronal marker] produced in chickens, anti-EAAT2 [an astrocyte marker] produced in sheep, anti-GFAP [anther astrocyte marker] produced in chicken, anti- CD11b/c [a microglial marker] produced in mice, and anti-MBP [an oligodendrocyte marker] produced in mice; all manufactured by Abcam) were added to the cells after blocking, followed by shaking for 1 h at room temperature. ..

    Produced:

    Article Title: A novel cyclic peptide (Naturido) modulates glia–neuron interactions in vitro and reverses ageing-related deficits in senescence-accelerated mice
    Article Snippet: .. Subsequently, for the identification of nuclei and cell types, DAPI for nuclear staining and primary antibodies (anti-MAP2 [a neuronal marker] produced in chickens, anti-EAAT2 [an astrocyte marker] produced in sheep, anti-GFAP [anther astrocyte marker] produced in chicken, anti- CD11b/c [a microglial marker] produced in mice, and anti-MBP [an oligodendrocyte marker] produced in mice; all manufactured by Abcam) were added to the cells after blocking, followed by shaking for 1 h at room temperature. ..

    Mouse Assay:

    Article Title: A novel cyclic peptide (Naturido) modulates glia–neuron interactions in vitro and reverses ageing-related deficits in senescence-accelerated mice
    Article Snippet: .. Subsequently, for the identification of nuclei and cell types, DAPI for nuclear staining and primary antibodies (anti-MAP2 [a neuronal marker] produced in chickens, anti-EAAT2 [an astrocyte marker] produced in sheep, anti-GFAP [anther astrocyte marker] produced in chicken, anti- CD11b/c [a microglial marker] produced in mice, and anti-MBP [an oligodendrocyte marker] produced in mice; all manufactured by Abcam) were added to the cells after blocking, followed by shaking for 1 h at room temperature. ..

    Blocking Assay:

    Article Title: A novel cyclic peptide (Naturido) modulates glia–neuron interactions in vitro and reverses ageing-related deficits in senescence-accelerated mice
    Article Snippet: .. Subsequently, for the identification of nuclei and cell types, DAPI for nuclear staining and primary antibodies (anti-MAP2 [a neuronal marker] produced in chickens, anti-EAAT2 [an astrocyte marker] produced in sheep, anti-GFAP [anther astrocyte marker] produced in chicken, anti- CD11b/c [a microglial marker] produced in mice, and anti-MBP [an oligodendrocyte marker] produced in mice; all manufactured by Abcam) were added to the cells after blocking, followed by shaking for 1 h at room temperature. ..

    Article Title: Cytoplasmic CPSF6 Regulates HIV-1 Capsid Trafficking and Infection in a Cyclophilin A-Dependent Manner
    Article Snippet: .. The membranes were blocked at ambient temperature for 1 h in bovine serum albumin (BSA) blocking buffers, followed by overnight incubation at 4°C with rabbit anti-maltose binding protein antibody (ab9084; Abcam) or rabbit anti-CPSF6 antibody (EPR12898; Abcam) and then a further hour with monoclonal anti-rabbit immunoglobulins-alkaline phosphatase antibody at ambient temperature. ..

    Article Title: Therapeutic Effect of Ginsenoside Rd on Experimental Autoimmune Encephalomyelitis Model Mice: Regulation of Inflammation and Treg/Th17 Cell Balance
    Article Snippet: .. The spinal cord sections in each group were firstly incubated in blocking solution (3% donkey serum, 0.3% Triton X-100 in PBS) for 30 min and stained with primary antibodies anti-MBP (dilution ratio, 1 : 200; Abcam, UK) at 4°C overnight. ..

    Incubation:

    Article Title: Cytoplasmic CPSF6 Regulates HIV-1 Capsid Trafficking and Infection in a Cyclophilin A-Dependent Manner
    Article Snippet: .. The membranes were blocked at ambient temperature for 1 h in bovine serum albumin (BSA) blocking buffers, followed by overnight incubation at 4°C with rabbit anti-maltose binding protein antibody (ab9084; Abcam) or rabbit anti-CPSF6 antibody (EPR12898; Abcam) and then a further hour with monoclonal anti-rabbit immunoglobulins-alkaline phosphatase antibody at ambient temperature. ..

    Article Title: Therapeutic Effect of Ginsenoside Rd on Experimental Autoimmune Encephalomyelitis Model Mice: Regulation of Inflammation and Treg/Th17 Cell Balance
    Article Snippet: .. The spinal cord sections in each group were firstly incubated in blocking solution (3% donkey serum, 0.3% Triton X-100 in PBS) for 30 min and stained with primary antibodies anti-MBP (dilution ratio, 1 : 200; Abcam, UK) at 4°C overnight. ..

    Binding Assay:

    Article Title: Cytoplasmic CPSF6 Regulates HIV-1 Capsid Trafficking and Infection in a Cyclophilin A-Dependent Manner
    Article Snippet: .. The membranes were blocked at ambient temperature for 1 h in bovine serum albumin (BSA) blocking buffers, followed by overnight incubation at 4°C with rabbit anti-maltose binding protein antibody (ab9084; Abcam) or rabbit anti-CPSF6 antibody (EPR12898; Abcam) and then a further hour with monoclonal anti-rabbit immunoglobulins-alkaline phosphatase antibody at ambient temperature. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Abcam anti mbp
    Loss of HMGNs affects oligodendrocyte development in mouse spinal cord. ( A–D ) Immunofluorescence showing decreased OLIG2 positive cells in both white matter ( A ) and gray matter ( C ) of DKO mouse spinal cords. ( B, D) Quantifications of four WT and four DKO mice. For each mouse, > 1500 cells from six (white matter) or four (gray matter) different regions of spinal cord sections were counted. ( E ) Immunofluorescence showing decreased <t>MBP</t> level in DKO spinal cords. ( F ) Quantification of panel J, eight WT and eight DKO images were quantified by ImageJ. ( G ) Western blots showing the expression of oligodendrocyte markers in WT and DKO mouse spinal cords. ( H ) Quantifications of panel L, expression levels normalized to <t>β-actin.</t> All the data are presented as mean ± SEM (* P
    Anti Mbp, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mbp/product/Abcam
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mbp - by Bioz Stars, 2021-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    Loss of HMGNs affects oligodendrocyte development in mouse spinal cord. ( A–D ) Immunofluorescence showing decreased OLIG2 positive cells in both white matter ( A ) and gray matter ( C ) of DKO mouse spinal cords. ( B, D) Quantifications of four WT and four DKO mice. For each mouse, > 1500 cells from six (white matter) or four (gray matter) different regions of spinal cord sections were counted. ( E ) Immunofluorescence showing decreased MBP level in DKO spinal cords. ( F ) Quantification of panel J, eight WT and eight DKO images were quantified by ImageJ. ( G ) Western blots showing the expression of oligodendrocyte markers in WT and DKO mouse spinal cords. ( H ) Quantifications of panel L, expression levels normalized to β-actin. All the data are presented as mean ± SEM (* P

    Journal: Nucleic Acids Research

    Article Title: Interplay between H1 and HMGN epigenetically regulates OLIG1 2 expression and oligodendrocyte differentiation

    doi: 10.1093/nar/gkw1222

    Figure Lengend Snippet: Loss of HMGNs affects oligodendrocyte development in mouse spinal cord. ( A–D ) Immunofluorescence showing decreased OLIG2 positive cells in both white matter ( A ) and gray matter ( C ) of DKO mouse spinal cords. ( B, D) Quantifications of four WT and four DKO mice. For each mouse, > 1500 cells from six (white matter) or four (gray matter) different regions of spinal cord sections were counted. ( E ) Immunofluorescence showing decreased MBP level in DKO spinal cords. ( F ) Quantification of panel J, eight WT and eight DKO images were quantified by ImageJ. ( G ) Western blots showing the expression of oligodendrocyte markers in WT and DKO mouse spinal cords. ( H ) Quantifications of panel L, expression levels normalized to β-actin. All the data are presented as mean ± SEM (* P

    Article Snippet: Anti-H3K27me3, anti-EZH2, anti-H3, anti-PGDFR-α, anti-CNPase, anti-PLP, anti-MBP and anti-β-actin antibodies were purchased from Abcam.

    Techniques: Immunofluorescence, Mouse Assay, Western Blot, Expressing

    Combination treatment with Sephin1 and IFN-β alleviates and delays clinical symptoms of EAE as well as reduces the oligodendrocytes and myelin loss during EAE course. ( A ) Clinical scores of C57BL/6 female mice immunized with MOG 35-55 /CFA to induce chronic EAE, treated with vehicle ( n = 12), 5000 U of IFN-β ( n = 12), 8 mg/kg of Sephin1 or Sephin1 combined with 5000 U of IFNβ ( n = 10) daily from PID 7 to the end of the study. ( B ) Average onset of disease, peak of disease and average peak score of all treatment groups. ( C and D ) Immunofluorescent staining for TPPP (green) and MBP (red) of lumbar spinal cord sections from PID 17 ( C ) and PID 30 ( D ). Scale bar = 100 μm. ( E ) Quantification of cells positive for TPPP in the lesion areas. ( F ) Average percentage of demyelinated areas/white matter areas measured from MBP staining. * P

    Journal: Brain

    Article Title: Sephin1, which prolongs the integrated stress response, is a promising therapeutic for multiple sclerosis

    doi: 10.1093/brain/awy322

    Figure Lengend Snippet: Combination treatment with Sephin1 and IFN-β alleviates and delays clinical symptoms of EAE as well as reduces the oligodendrocytes and myelin loss during EAE course. ( A ) Clinical scores of C57BL/6 female mice immunized with MOG 35-55 /CFA to induce chronic EAE, treated with vehicle ( n = 12), 5000 U of IFN-β ( n = 12), 8 mg/kg of Sephin1 or Sephin1 combined with 5000 U of IFNβ ( n = 10) daily from PID 7 to the end of the study. ( B ) Average onset of disease, peak of disease and average peak score of all treatment groups. ( C and D ) Immunofluorescent staining for TPPP (green) and MBP (red) of lumbar spinal cord sections from PID 17 ( C ) and PID 30 ( D ). Scale bar = 100 μm. ( E ) Quantification of cells positive for TPPP in the lesion areas. ( F ) Average percentage of demyelinated areas/white matter areas measured from MBP staining. * P

    Article Snippet: Primary antibodies include the following: anti-MBP (Abcam, ab24567, 1:700), anti-TPPP (Thermo Fisher Scientific, PA5-19243, 1:100), anti-p-eIF2α (Abcam, ab32157, 1:100; Thermo Fisher Scientific, MA5-15133, 1:50), anti-CD3 (Santa Cruz, sc-18843, 1:200), anti-CD11b (Bio-Rad, MCA711, 1:50), anti-NG2 (Millipore, Ab5320, 1:100), anti-neurofilament (Millipore, AB5539, 1:500), anti-GFAP (Millipore, AB5541, 1:200), anti-caspase 3 (Abcam, AB2302, 1:50), anti-Ki67 (Abcam, AB15580, 1:100), anti-PDGFR-alpha (BD Biosciences, 558774, 1:100), and anti-SOX10 (R & D, AF2864, 1:100).

    Techniques: Mouse Assay, Staining

    AATYK knockdown does not affect the survival but maintains proliferation of OPCs. A Ki67 immunostaining showed there were more Ki67 + and GFP + co-labeled cells in the sh- AATYK -treated OPCs than in the control. B Percentages of Ki67 + and GFP + co-labelled OLs in GFP + cells among RNAi- AATYK cells were significantly higher than in the control. C – D Compared to control cells, the expression of MBP protein was significantly decreased in shRNA- AATYK -treated cells and there was no difference in caspase3 between the two groups. Scale bar, 100 μm.

    Journal: Neuroscience Bulletin

    Article Title: AATYK is a Novel Regulator of Oligodendrocyte Differentiation and Myelination

    doi: 10.1007/s12264-018-0218-6

    Figure Lengend Snippet: AATYK knockdown does not affect the survival but maintains proliferation of OPCs. A Ki67 immunostaining showed there were more Ki67 + and GFP + co-labeled cells in the sh- AATYK -treated OPCs than in the control. B Percentages of Ki67 + and GFP + co-labelled OLs in GFP + cells among RNAi- AATYK cells were significantly higher than in the control. C – D Compared to control cells, the expression of MBP protein was significantly decreased in shRNA- AATYK -treated cells and there was no difference in caspase3 between the two groups. Scale bar, 100 μm.

    Article Snippet: SDS-PAGE gels were subsequently detected with anti-MBP (Abcam, 1:2 000), anti-caspase3 (Millipore, 1:1 000), and anti-GAPDH (Sigma, 1:5 000) antibodies.

    Techniques: Immunostaining, Labeling, Expressing, shRNA

    The interactions between NtWRKY6 and five tobacco MAPKs, wound-induced protein kinase (WIPK), salicylic acid-induced protein kinase (SIPK), NTF4-1, NTF4-2, and NRK1. ( A ) In vivo interaction between WRKY6 and NtMAPKs as shown by BiFC analysis. NtWRKY6-YFPN and NtMAPKs-YFPC were transiently co-expressed in N. benthamiana line H2B-RFP, of which the nuclei were marked with RFP fusion protein. Photos were imaged at 48 h using a Zeiss LSM710 confoc al microscope. Columns from left to right represent YFP fluorescence, bright field, RFP fluorescence, and YFP/RFP/bright field overlay. Scale bars: 50 μm. ( B ) In vitro interaction between NtWRKY6 and NtMAPKs as shown by pull-down assay. Proteins GST, NtMAPKs-GST, MBP, and NtWRKY6-MBP were expressed via prokaryotic expression, and purified using glutathione agarose beads or Amylose resin. GST or NtMAPK-GST fusion proteins were used to pull down MBP or NtWRKY6-MBP fusion proteins. Binding proteins were analyzed via SDS-PAGE and Western blot assays using anti-MBP antibodies. At the start, samples (Input) were stained with Coomassie blue solution. GST and MBP proteins were used as negative controls.

    Journal: Insects

    Article Title: WRKY Transcription Factors in Nicotiana tabacum Modulate Plant Immunity against Whitefly via Interacting with MAPK Cascade Pathways

    doi: 10.3390/insects12010016

    Figure Lengend Snippet: The interactions between NtWRKY6 and five tobacco MAPKs, wound-induced protein kinase (WIPK), salicylic acid-induced protein kinase (SIPK), NTF4-1, NTF4-2, and NRK1. ( A ) In vivo interaction between WRKY6 and NtMAPKs as shown by BiFC analysis. NtWRKY6-YFPN and NtMAPKs-YFPC were transiently co-expressed in N. benthamiana line H2B-RFP, of which the nuclei were marked with RFP fusion protein. Photos were imaged at 48 h using a Zeiss LSM710 confoc al microscope. Columns from left to right represent YFP fluorescence, bright field, RFP fluorescence, and YFP/RFP/bright field overlay. Scale bars: 50 μm. ( B ) In vitro interaction between NtWRKY6 and NtMAPKs as shown by pull-down assay. Proteins GST, NtMAPKs-GST, MBP, and NtWRKY6-MBP were expressed via prokaryotic expression, and purified using glutathione agarose beads or Amylose resin. GST or NtMAPK-GST fusion proteins were used to pull down MBP or NtWRKY6-MBP fusion proteins. Binding proteins were analyzed via SDS-PAGE and Western blot assays using anti-MBP antibodies. At the start, samples (Input) were stained with Coomassie blue solution. GST and MBP proteins were used as negative controls.

    Article Snippet: The beads were boiled with SDS loading buffer for 10 min. Proteins were separated using SDS-PAGE and detected using Western blot with an anti-MBP tag mouse monoclonal antibody (Abcam, Cambridge, UK).

    Techniques: In Vivo, Bimolecular Fluorescence Complementation Assay, Microscopy, Fluorescence, In Vitro, Pull Down Assay, Expressing, Purification, Binding Assay, SDS Page, Western Blot, Staining