Structured Review

Proteintech mat2a
<t>MAT2A</t> was decreased and MAT2B was increased in the hippocampus post-rmTBI. MAT2A and MATB were assessed via capillary electrophoresis. (A) MAT2A (catalytic subunit) had a trending decrease at 7 days (blue bar) relative to SHAM (black bar). (B) MAT2B (regulatory subunit) was significantly enhanced 7 days (blue bar) post-rmTBI relative to SHAM (black bar). (C–D) MAT2A and MAT2B were unchanged in the cerebral cortex post-rmTBI. Results were expressed as mean ± SEM. *p ≤ 0.05, evaluated by one-way ANOVA with Tukey’s post-hoc test, (n = 3–6).
Mat2a, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mat2a - by Bioz Stars, 2024-04
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1) Product Images from "A role for protein arginine methyltransferase 7 in repetitive and mild traumatic brain injury"

Article Title: A role for protein arginine methyltransferase 7 in repetitive and mild traumatic brain injury

Journal: Neurochemistry international

doi: 10.1016/j.neuint.2023.105524

MAT2A was decreased and MAT2B was increased in the hippocampus post-rmTBI. MAT2A and MATB were assessed via capillary electrophoresis. (A) MAT2A (catalytic subunit) had a trending decrease at 7 days (blue bar) relative to SHAM (black bar). (B) MAT2B (regulatory subunit) was significantly enhanced 7 days (blue bar) post-rmTBI relative to SHAM (black bar). (C–D) MAT2A and MAT2B were unchanged in the cerebral cortex post-rmTBI. Results were expressed as mean ± SEM. *p ≤ 0.05, evaluated by one-way ANOVA with Tukey’s post-hoc test, (n = 3–6).
Figure Legend Snippet: MAT2A was decreased and MAT2B was increased in the hippocampus post-rmTBI. MAT2A and MATB were assessed via capillary electrophoresis. (A) MAT2A (catalytic subunit) had a trending decrease at 7 days (blue bar) relative to SHAM (black bar). (B) MAT2B (regulatory subunit) was significantly enhanced 7 days (blue bar) post-rmTBI relative to SHAM (black bar). (C–D) MAT2A and MAT2B were unchanged in the cerebral cortex post-rmTBI. Results were expressed as mean ± SEM. *p ≤ 0.05, evaluated by one-way ANOVA with Tukey’s post-hoc test, (n = 3–6).

Techniques Used: Electrophoresis

MAT2A inhibitor reduced PRMT7 protein expression and MMA production. PF-9366 allosteric inhibitor was used (25 μM) to inhibit MAT2A in HT22 cells for 24 hrs. Inhibition of MAT2A significantly decreased (A) PRMT7 protein expression (B) decreased mono-methylarginine (MMa) production (C) increased MAT2A protein expression and significantly decreased (D) MAT2B protein expression. Results were expressed as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 evaluated by student’s t-test. (n = 3).
Figure Legend Snippet: MAT2A inhibitor reduced PRMT7 protein expression and MMA production. PF-9366 allosteric inhibitor was used (25 μM) to inhibit MAT2A in HT22 cells for 24 hrs. Inhibition of MAT2A significantly decreased (A) PRMT7 protein expression (B) decreased mono-methylarginine (MMa) production (C) increased MAT2A protein expression and significantly decreased (D) MAT2B protein expression. Results were expressed as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 evaluated by student’s t-test. (n = 3).

Techniques Used: Expressing, Inhibition


Structured Review

Proteintech mat2a
<t>MAT2A</t> was decreased and MAT2B was increased in the hippocampus post-rmTBI. MAT2A and MATB were assessed via capillary electrophoresis. (A) MAT2A (catalytic subunit) had a trending decrease at 7 days (blue bar) relative to SHAM (black bar). (B) MAT2B (regulatory subunit) was significantly enhanced 7 days (blue bar) post-rmTBI relative to SHAM (black bar). (C–D) MAT2A and MAT2B were unchanged in the cerebral cortex post-rmTBI. Results were expressed as mean ± SEM. *p ≤ 0.05, evaluated by one-way ANOVA with Tukey’s post-hoc test, (n = 3–6).
Mat2a, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mat2a/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mat2a - by Bioz Stars, 2024-04
86/100 stars

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1) Product Images from "A role for protein arginine methyltransferase 7 in repetitive and mild traumatic brain injury"

Article Title: A role for protein arginine methyltransferase 7 in repetitive and mild traumatic brain injury

Journal: Neurochemistry international

doi: 10.1016/j.neuint.2023.105524

MAT2A was decreased and MAT2B was increased in the hippocampus post-rmTBI. MAT2A and MATB were assessed via capillary electrophoresis. (A) MAT2A (catalytic subunit) had a trending decrease at 7 days (blue bar) relative to SHAM (black bar). (B) MAT2B (regulatory subunit) was significantly enhanced 7 days (blue bar) post-rmTBI relative to SHAM (black bar). (C–D) MAT2A and MAT2B were unchanged in the cerebral cortex post-rmTBI. Results were expressed as mean ± SEM. *p ≤ 0.05, evaluated by one-way ANOVA with Tukey’s post-hoc test, (n = 3–6).
Figure Legend Snippet: MAT2A was decreased and MAT2B was increased in the hippocampus post-rmTBI. MAT2A and MATB were assessed via capillary electrophoresis. (A) MAT2A (catalytic subunit) had a trending decrease at 7 days (blue bar) relative to SHAM (black bar). (B) MAT2B (regulatory subunit) was significantly enhanced 7 days (blue bar) post-rmTBI relative to SHAM (black bar). (C–D) MAT2A and MAT2B were unchanged in the cerebral cortex post-rmTBI. Results were expressed as mean ± SEM. *p ≤ 0.05, evaluated by one-way ANOVA with Tukey’s post-hoc test, (n = 3–6).

Techniques Used: Electrophoresis

MAT2A inhibitor reduced PRMT7 protein expression and MMA production. PF-9366 allosteric inhibitor was used (25 μM) to inhibit MAT2A in HT22 cells for 24 hrs. Inhibition of MAT2A significantly decreased (A) PRMT7 protein expression (B) decreased mono-methylarginine (MMa) production (C) increased MAT2A protein expression and significantly decreased (D) MAT2B protein expression. Results were expressed as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 evaluated by student’s t-test. (n = 3).
Figure Legend Snippet: MAT2A inhibitor reduced PRMT7 protein expression and MMA production. PF-9366 allosteric inhibitor was used (25 μM) to inhibit MAT2A in HT22 cells for 24 hrs. Inhibition of MAT2A significantly decreased (A) PRMT7 protein expression (B) decreased mono-methylarginine (MMa) production (C) increased MAT2A protein expression and significantly decreased (D) MAT2B protein expression. Results were expressed as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 evaluated by student’s t-test. (n = 3).

Techniques Used: Expressing, Inhibition


Structured Review

Proteintech rabbit anti mat2a antibody
Rabbit Anti Mat2a Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti mat2a antibody/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit anti mat2a antibody - by Bioz Stars, 2024-04
86/100 stars

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Structured Review

Proteintech antibodies to mat2a
Primer sequences used for real-time PCR.
Antibodies To Mat2a, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies to mat2a/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
antibodies to mat2a - by Bioz Stars, 2024-04
86/100 stars

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1) Product Images from "MAT2A inhibition suppresses inflammation in Porphyromonas gingivalis -infected human gingival fibroblasts"

Article Title: MAT2A inhibition suppresses inflammation in Porphyromonas gingivalis -infected human gingival fibroblasts

Journal: Journal of Oral Microbiology

doi: 10.1080/20002297.2023.2292375

Primer sequences used for real-time PCR.
Figure Legend Snippet: Primer sequences used for real-time PCR.

Techniques Used: Sequencing

MAT2A expression was upregulated in inflamed gingival tissues.
Figure Legend Snippet: MAT2A expression was upregulated in inflamed gingival tissues.

Techniques Used: Expressing

MAT2A inhibition decreased P. gingivalis -induced inflammatory responses in hGfs.
Figure Legend Snippet: MAT2A inhibition decreased P. gingivalis -induced inflammatory responses in hGfs.

Techniques Used: Inhibition

MAT2A-knockdown reduced inflammation in P. gingivalis -infected hGFs.
Figure Legend Snippet: MAT2A-knockdown reduced inflammation in P. gingivalis -infected hGFs.

Techniques Used: Infection

NF-κB/MAPK pathway was involved in the enhanced MAT2A expression.
Figure Legend Snippet: NF-κB/MAPK pathway was involved in the enhanced MAT2A expression.

Techniques Used: Expressing


Structured Review

Proteintech anti mat2a
Primer sequences used for real-time PCR.
Anti Mat2a, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti mat2a/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti mat2a - by Bioz Stars, 2024-04
86/100 stars

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1) Product Images from "MAT2A inhibition suppresses inflammation in Porphyromonas gingivalis -infected human gingival fibroblasts"

Article Title: MAT2A inhibition suppresses inflammation in Porphyromonas gingivalis -infected human gingival fibroblasts

Journal: Journal of Oral Microbiology

doi: 10.1080/20002297.2023.2292375

Primer sequences used for real-time PCR.
Figure Legend Snippet: Primer sequences used for real-time PCR.

Techniques Used: Sequencing

MAT2A expression was upregulated in inflamed gingival tissues.
Figure Legend Snippet: MAT2A expression was upregulated in inflamed gingival tissues.

Techniques Used: Expressing

MAT2A inhibition decreased P. gingivalis -induced inflammatory responses in hGfs.
Figure Legend Snippet: MAT2A inhibition decreased P. gingivalis -induced inflammatory responses in hGfs.

Techniques Used: Inhibition

MAT2A-knockdown reduced inflammation in P. gingivalis -infected hGFs.
Figure Legend Snippet: MAT2A-knockdown reduced inflammation in P. gingivalis -infected hGFs.

Techniques Used: Infection

NF-κB/MAPK pathway was involved in the enhanced MAT2A expression.
Figure Legend Snippet: NF-κB/MAPK pathway was involved in the enhanced MAT2A expression.

Techniques Used: Expressing


Structured Review

Proteintech mat2a antibody
Mat2a Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mat2a antibody/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mat2a antibody - by Bioz Stars, 2024-04
86/100 stars

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Structured Review

Proteintech mat2a
Mat2a, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mat2a/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mat2a - by Bioz Stars, 2024-04
86/100 stars

Images


Structured Review

Proteintech mat2a
A , B Metabolomics were performed in T24 cells versus T24-CR cells. Metabolites related with amino acid metabolism were further selected. C KEGG pathway enrichment of the metabolomics was applied to identified the enriched metabonomic pathway between T24 cells and T24-CR cells. D The schema of <t>MAT2A</t> regulated methionine metabolism pathway. Differentiated metabolites between T24 cells and T24-CR cells were marked with arrows. *** p < 0.001 versus T24. E Proteomics were performed in T24 cells versus T24-CR cells. F KEGG pathway enrichment of the proteomics was applied to identified the enriched pathway between T24 cells and T24-CR cells. G Integrated analysis of the correlation between the metabolomics and proteomics. H Western blot analysis of the enzymes and stem cell markers of the BCa cells. I Western blot analysis of the histone methylated markers and stem cell markers of the BCa cells with 5 μM FIDAS treatment or MAT2A silencing. J Spheroid assay was performed to analyze spheroid/organoid forming ability of the relatively treated T24-CR cells.Relative cell proliferation was measured through CCK-8 assay. *** p < 0.001 versus T24CR. K The tumor volume of 5 × 10 4 T24-CR cells with relative treatment. The successful rate of tumor formation and weight were calculated (10 mice were enrolled in each treatment group). L The extreme limiting dilution experiment was performed to discover the in vivo tumorigenicity of T24-CR cells with relatively treatment. M Cell viability of T24-CR cells with different treatments in cisplatin containing medium.
Mat2a, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mat2a/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mat2a - by Bioz Stars, 2024-04
86/100 stars

Images

1) Product Images from "Methionine orchestrates the metabolism vulnerability in cisplatin resistant bladder cancer microenvironment"

Article Title: Methionine orchestrates the metabolism vulnerability in cisplatin resistant bladder cancer microenvironment

Journal: Cell Death & Disease

doi: 10.1038/s41419-023-06050-1

A , B Metabolomics were performed in T24 cells versus T24-CR cells. Metabolites related with amino acid metabolism were further selected. C KEGG pathway enrichment of the metabolomics was applied to identified the enriched metabonomic pathway between T24 cells and T24-CR cells. D The schema of MAT2A regulated methionine metabolism pathway. Differentiated metabolites between T24 cells and T24-CR cells were marked with arrows. *** p < 0.001 versus T24. E Proteomics were performed in T24 cells versus T24-CR cells. F KEGG pathway enrichment of the proteomics was applied to identified the enriched pathway between T24 cells and T24-CR cells. G Integrated analysis of the correlation between the metabolomics and proteomics. H Western blot analysis of the enzymes and stem cell markers of the BCa cells. I Western blot analysis of the histone methylated markers and stem cell markers of the BCa cells with 5 μM FIDAS treatment or MAT2A silencing. J Spheroid assay was performed to analyze spheroid/organoid forming ability of the relatively treated T24-CR cells.Relative cell proliferation was measured through CCK-8 assay. *** p < 0.001 versus T24CR. K The tumor volume of 5 × 10 4 T24-CR cells with relative treatment. The successful rate of tumor formation and weight were calculated (10 mice were enrolled in each treatment group). L The extreme limiting dilution experiment was performed to discover the in vivo tumorigenicity of T24-CR cells with relatively treatment. M Cell viability of T24-CR cells with different treatments in cisplatin containing medium.
Figure Legend Snippet: A , B Metabolomics were performed in T24 cells versus T24-CR cells. Metabolites related with amino acid metabolism were further selected. C KEGG pathway enrichment of the metabolomics was applied to identified the enriched metabonomic pathway between T24 cells and T24-CR cells. D The schema of MAT2A regulated methionine metabolism pathway. Differentiated metabolites between T24 cells and T24-CR cells were marked with arrows. *** p < 0.001 versus T24. E Proteomics were performed in T24 cells versus T24-CR cells. F KEGG pathway enrichment of the proteomics was applied to identified the enriched pathway between T24 cells and T24-CR cells. G Integrated analysis of the correlation between the metabolomics and proteomics. H Western blot analysis of the enzymes and stem cell markers of the BCa cells. I Western blot analysis of the histone methylated markers and stem cell markers of the BCa cells with 5 μM FIDAS treatment or MAT2A silencing. J Spheroid assay was performed to analyze spheroid/organoid forming ability of the relatively treated T24-CR cells.Relative cell proliferation was measured through CCK-8 assay. *** p < 0.001 versus T24CR. K The tumor volume of 5 × 10 4 T24-CR cells with relative treatment. The successful rate of tumor formation and weight were calculated (10 mice were enrolled in each treatment group). L The extreme limiting dilution experiment was performed to discover the in vivo tumorigenicity of T24-CR cells with relatively treatment. M Cell viability of T24-CR cells with different treatments in cisplatin containing medium.

Techniques Used: Western Blot, Methylation, CCK-8 Assay, In Vivo

A Relative differential circRNA expression between T24 cells versus T24-CR cells. B Volcano plot was performed to reveal the differential expressed circRNAs. C Relative MAT2A expression of the T24-CR cells with the indicated circRNA knock down or up-regulated. D The tumor volume formed from 5 × 10 4 T24-CR cells transfected with relative circRNA silencing or over-expression plasmid. E The successful rate of tumor formation and weight formed from 5 × 10 4 T24-CR cells transfected with relative circRNA silencing or over-expression plasmid (5 mice were enrolled in each treatment group). F Genomic loci and structure of circARHGAP10. G Expression of circular/linear forms of transcripts in BCa cell with RNase R treatment. H Expression of circular/linear forms of transcripts in BCa cell after treatment by actinomycin D. *** p < 0.001 versus circARHGAP10 group. I Metabolomics were performed in T24-CR-LV-NC cells versus T24-CR-LV-circARHGAP10-OE cells. J Western blot analysis of MAT2A, the histone methylated markers and stem cell markers of T24, T24-si-circARHGAP10, T24-CR, T24-CR-LV-circARHGAP10 cells. K Cell viability of T24, T24-si-circARHGAP10, T24-CR, T24-CR-LV-circARHGAP10 cells in cisplatin containing medium. L Sphere formation assay was used to analyze the spheroid/organoid formation ability of the T24, T24-si-circARHGAP10, T24-CR, T24-CR-LV-circARHGAP10 cells. Relative cell proliferation was measured through CCK-8 assay. *** p < 0.001 versus T24CR. M The tumor volume formed from 5×10 4 T24, T24-si-circARHGAP10, T24-CR, T24-CR-LV-circARHGAP10 cells. N The successful rate of tumor formation and weight formed from 5×10 4 T24, T24-si-circ ARHGAP10, T24-CR, T24-CR-LV-circARHGAP10 cells (5 mice were enrolled in each treatment group). O Fluorescence in situ hybridization (FISH) of circARHGAP10 in our BCa tissue microarray (TMA). P Kaplan-Meier plot shows the correlation of circARHGAP10 with overall survival. Q circARHGAP10 expression levels in normal tissue and tumor tissue of BCa TMA. R circARHGAP10 expression in BCa tissue samples from recurrence after 6 months ( n = 9) or recurrence in 6 months ( n = 8). S Relative circARHGAP10 expression from paired BCa tissue samples from patients received cisplatin based chemotherapy ( n = 5).
Figure Legend Snippet: A Relative differential circRNA expression between T24 cells versus T24-CR cells. B Volcano plot was performed to reveal the differential expressed circRNAs. C Relative MAT2A expression of the T24-CR cells with the indicated circRNA knock down or up-regulated. D The tumor volume formed from 5 × 10 4 T24-CR cells transfected with relative circRNA silencing or over-expression plasmid. E The successful rate of tumor formation and weight formed from 5 × 10 4 T24-CR cells transfected with relative circRNA silencing or over-expression plasmid (5 mice were enrolled in each treatment group). F Genomic loci and structure of circARHGAP10. G Expression of circular/linear forms of transcripts in BCa cell with RNase R treatment. H Expression of circular/linear forms of transcripts in BCa cell after treatment by actinomycin D. *** p < 0.001 versus circARHGAP10 group. I Metabolomics were performed in T24-CR-LV-NC cells versus T24-CR-LV-circARHGAP10-OE cells. J Western blot analysis of MAT2A, the histone methylated markers and stem cell markers of T24, T24-si-circARHGAP10, T24-CR, T24-CR-LV-circARHGAP10 cells. K Cell viability of T24, T24-si-circARHGAP10, T24-CR, T24-CR-LV-circARHGAP10 cells in cisplatin containing medium. L Sphere formation assay was used to analyze the spheroid/organoid formation ability of the T24, T24-si-circARHGAP10, T24-CR, T24-CR-LV-circARHGAP10 cells. Relative cell proliferation was measured through CCK-8 assay. *** p < 0.001 versus T24CR. M The tumor volume formed from 5×10 4 T24, T24-si-circARHGAP10, T24-CR, T24-CR-LV-circARHGAP10 cells. N The successful rate of tumor formation and weight formed from 5×10 4 T24, T24-si-circ ARHGAP10, T24-CR, T24-CR-LV-circARHGAP10 cells (5 mice were enrolled in each treatment group). O Fluorescence in situ hybridization (FISH) of circARHGAP10 in our BCa tissue microarray (TMA). P Kaplan-Meier plot shows the correlation of circARHGAP10 with overall survival. Q circARHGAP10 expression levels in normal tissue and tumor tissue of BCa TMA. R circARHGAP10 expression in BCa tissue samples from recurrence after 6 months ( n = 9) or recurrence in 6 months ( n = 8). S Relative circARHGAP10 expression from paired BCa tissue samples from patients received cisplatin based chemotherapy ( n = 5).

Techniques Used: Expressing, Transfection, Over Expression, Plasmid Preparation, Western Blot, Methylation, Tube Formation Assay, CCK-8 Assay, Fluorescence, In Situ Hybridization, Microarray

A RIP assays were performed with an anti-AGO2 antibody in T24-CR cells to analyze circARHGAP10 enrichment. *** p < 0.001 versus input group. ( B ) Analysis for lineal and circular ARHGAP10 enrichment. RNA pull down was performed with sense probe which targeting the back splice junction site of circARHGAP10. *** p < 0.001 versus anti-sense group. C Sense probe and antisense probe were incubated with cell lysates of T24-CR -LV-circARHGAP10 cells for silver staining. D The peptide segment diagram of MAT2A was identified by mass spectrometry from RNA pulldown. E Sequence of identical peptides of MAT2A identified by mass spectrometry. F Co-localization of circARHGAP10 with MAT2A in BCa cells. Scale bar = 10 μm. G The interaction between MAT2A and circARHGAP10. H Protein levels of MAT2A in BCa cells with circARHGAP10 overexpression. I Protein levels of MAT2A in circARHGAP10 overexpressed BCa cells with MG132 (20 μM) treatment for 10 h. J Relative protein levels of MAT2A and GAPDH in BCa cells treated with 200 μM cycloheximide (CHX) at different time points. K Co-IP experiment was performed to determine the ubiquitination modification level of MAT2A in BCa cells. L Co-IP experiment was performed to determine the ubiquitination modification level of MAT2A in 293 T cells. M Relative expression of MAT2A and circARHGAP10. N The contingency of the correlation of the staining intensity of MAT2A and circARHGAP10 analyzed with chi-squared test.
Figure Legend Snippet: A RIP assays were performed with an anti-AGO2 antibody in T24-CR cells to analyze circARHGAP10 enrichment. *** p < 0.001 versus input group. ( B ) Analysis for lineal and circular ARHGAP10 enrichment. RNA pull down was performed with sense probe which targeting the back splice junction site of circARHGAP10. *** p < 0.001 versus anti-sense group. C Sense probe and antisense probe were incubated with cell lysates of T24-CR -LV-circARHGAP10 cells for silver staining. D The peptide segment diagram of MAT2A was identified by mass spectrometry from RNA pulldown. E Sequence of identical peptides of MAT2A identified by mass spectrometry. F Co-localization of circARHGAP10 with MAT2A in BCa cells. Scale bar = 10 μm. G The interaction between MAT2A and circARHGAP10. H Protein levels of MAT2A in BCa cells with circARHGAP10 overexpression. I Protein levels of MAT2A in circARHGAP10 overexpressed BCa cells with MG132 (20 μM) treatment for 10 h. J Relative protein levels of MAT2A and GAPDH in BCa cells treated with 200 μM cycloheximide (CHX) at different time points. K Co-IP experiment was performed to determine the ubiquitination modification level of MAT2A in BCa cells. L Co-IP experiment was performed to determine the ubiquitination modification level of MAT2A in 293 T cells. M Relative expression of MAT2A and circARHGAP10. N The contingency of the correlation of the staining intensity of MAT2A and circARHGAP10 analyzed with chi-squared test.

Techniques Used: Incubation, Silver Staining, Mass Spectrometry, Sequencing, Over Expression, Co-Immunoprecipitation Assay, Modification, Expressing, Staining

A Intersection of the proteomics between T24 and T24-CR cells and the mass spectrometry analysis of circARHGAP10 RNA-pulldown. B Differential protein levels of ISG15, USP5, USP14 and TRIM25 of T24 and T24-CR cells identified with proteomics analysis. C Binding of circARHGAP10 with TRIM25 in BCa cells. D Co-localization of TRIM25 with MAT2A in BCa cells. Scale bar = 10 μm. E Co-IP validated TRIM25 binding with MAT2A in T24-CR cells. F Molecular docking of the protein structure of TRIM25 and MAT2A. G MAT2A protein expression in BCa cells with knockdown of TRIM25. H Protein levels of MAT2A in BCa cells with the over-expression of TRIM25 or TRIM25ΔRBD. I Co-IP experiment was performed to identify the ubiquitination modification level of MAT2A in BCa cells with the over-expression of TRIM25 or TRIM25ΔRBD. J Co-IP experiment was performed to identify the ubiquitination level of MAT2A in 293 T cells with the over-expression of TRIM25 or TRIM25ΔRBD. K Co-IP experiment was performed to identify the ubiquitination level of MAT2A in 293 T cells with the over-expression of HA-Ubiquitin or the indicated mutants. L Co-IP experiment was performed to identify the ubiquitination level of MAT2A in BCa cells with the over-expression of TRIM25 or the silencing of circARHGAP10. M The protein level of MAT2A in BCa cells with the over-expression of TRIM25 or the silencing of circARHGAP10. N Co-IP experiment was performed to validate the correlation of circARHGAP10 with binding of TRIM25 with MAT2A in T24-CR cells. RNase A (10 μg/ml) and RNase R (100 U/ml) were the indicated treatment concentration.
Figure Legend Snippet: A Intersection of the proteomics between T24 and T24-CR cells and the mass spectrometry analysis of circARHGAP10 RNA-pulldown. B Differential protein levels of ISG15, USP5, USP14 and TRIM25 of T24 and T24-CR cells identified with proteomics analysis. C Binding of circARHGAP10 with TRIM25 in BCa cells. D Co-localization of TRIM25 with MAT2A in BCa cells. Scale bar = 10 μm. E Co-IP validated TRIM25 binding with MAT2A in T24-CR cells. F Molecular docking of the protein structure of TRIM25 and MAT2A. G MAT2A protein expression in BCa cells with knockdown of TRIM25. H Protein levels of MAT2A in BCa cells with the over-expression of TRIM25 or TRIM25ΔRBD. I Co-IP experiment was performed to identify the ubiquitination modification level of MAT2A in BCa cells with the over-expression of TRIM25 or TRIM25ΔRBD. J Co-IP experiment was performed to identify the ubiquitination level of MAT2A in 293 T cells with the over-expression of TRIM25 or TRIM25ΔRBD. K Co-IP experiment was performed to identify the ubiquitination level of MAT2A in 293 T cells with the over-expression of HA-Ubiquitin or the indicated mutants. L Co-IP experiment was performed to identify the ubiquitination level of MAT2A in BCa cells with the over-expression of TRIM25 or the silencing of circARHGAP10. M The protein level of MAT2A in BCa cells with the over-expression of TRIM25 or the silencing of circARHGAP10. N Co-IP experiment was performed to validate the correlation of circARHGAP10 with binding of TRIM25 with MAT2A in T24-CR cells. RNase A (10 μg/ml) and RNase R (100 U/ml) were the indicated treatment concentration.

Techniques Used: Mass Spectrometry, Binding Assay, Co-Immunoprecipitation Assay, Expressing, Over Expression, Modification, Concentration Assay

A 1 × 10 7 T24-CR-luc-LV-NC or T24-CR-luc-LV-circ cells were injected subcutaneously in 4-week-old nude male athymic BALB/c mice fed with control or MR diets under 6 mg/kg cisplatin treatment (six mice were enrolled in each treatment group). B Tumor volume was monitored after 6 weeks of treatment. C Tumor weight were measured separately in each group. Data are presented as the mean ± SD. *** p < 0.001 versus LV-NC group. D Tumor volumes were measured separately in each group. Data are presented as the mean ± SD. *** p < 0.001 versus LV-NC group. E IHC staining of the expression of Ki-67, MAT2A and CD44 in cisplatin resistant BCa cell xenograft model. F Patient-derived xenograft (PDX) models were established with two fresh fragments of tissue of BCa patients transplanted subcutaneously into 4-week-old NOD/SCID mice. The 2nd PDX generation were injected with 5 nmol in cholesterol-conjugated si-NC or si-circARHGAP10 fed with control or MR diets under 6 mg/kg cisplatin treatment (six mice were enrolled in each treatment group). G Tumor volumes were measured separately in each group. Data are presented as the mean ± SD. *** p < 0.001, ** p < 0.01 versus PDX-si-NC group. H Tumor weights were measured separately in each group. Data are presented as the mean ± SD. *** p < 0.001, * p < 0.05 versus PDX-si-NC group. I IHC staining of the expression of Ki-67, MAT2A and CD44 in patient-derived xenograft model. J 1 × 10 5 T24-CR-luc-LV-NC or T24-CR-luc-LV-circ cells were injected intravenously into the tails of 4-week-old nude male athymic BALB/c mice fed with control or MR diets under 6 mg/kg cisplatin treatment (6 mice were enrolled in each treatment group). K H&E staining of lung metastatic nodules with indicated treatment. L Bioluminescence was used to detect the metastasis ability of T24-CR-luc cells with indicated treatment. Data are presented as mean ± SD. *** p < 0.001 versus LV-NC group. M Numbers of lung metastatic nodules with indicated treatment. Data are presented as mean ± SD. *** p < 0.001 versus LV-NC group.
Figure Legend Snippet: A 1 × 10 7 T24-CR-luc-LV-NC or T24-CR-luc-LV-circ cells were injected subcutaneously in 4-week-old nude male athymic BALB/c mice fed with control or MR diets under 6 mg/kg cisplatin treatment (six mice were enrolled in each treatment group). B Tumor volume was monitored after 6 weeks of treatment. C Tumor weight were measured separately in each group. Data are presented as the mean ± SD. *** p < 0.001 versus LV-NC group. D Tumor volumes were measured separately in each group. Data are presented as the mean ± SD. *** p < 0.001 versus LV-NC group. E IHC staining of the expression of Ki-67, MAT2A and CD44 in cisplatin resistant BCa cell xenograft model. F Patient-derived xenograft (PDX) models were established with two fresh fragments of tissue of BCa patients transplanted subcutaneously into 4-week-old NOD/SCID mice. The 2nd PDX generation were injected with 5 nmol in cholesterol-conjugated si-NC or si-circARHGAP10 fed with control or MR diets under 6 mg/kg cisplatin treatment (six mice were enrolled in each treatment group). G Tumor volumes were measured separately in each group. Data are presented as the mean ± SD. *** p < 0.001, ** p < 0.01 versus PDX-si-NC group. H Tumor weights were measured separately in each group. Data are presented as the mean ± SD. *** p < 0.001, * p < 0.05 versus PDX-si-NC group. I IHC staining of the expression of Ki-67, MAT2A and CD44 in patient-derived xenograft model. J 1 × 10 5 T24-CR-luc-LV-NC or T24-CR-luc-LV-circ cells were injected intravenously into the tails of 4-week-old nude male athymic BALB/c mice fed with control or MR diets under 6 mg/kg cisplatin treatment (6 mice were enrolled in each treatment group). K H&E staining of lung metastatic nodules with indicated treatment. L Bioluminescence was used to detect the metastasis ability of T24-CR-luc cells with indicated treatment. Data are presented as mean ± SD. *** p < 0.001 versus LV-NC group. M Numbers of lung metastatic nodules with indicated treatment. Data are presented as mean ± SD. *** p < 0.001 versus LV-NC group.

Techniques Used: Injection, Immunohistochemistry, Expressing, Derivative Assay, Staining

A Effects of BCH or MeAIB on supernatant of T24CR cells affected function of CD8 + T cell. Data are presented as the mean ± SD. *** p < 0.001, ** p < 0.01 versus Sup. B RT-qPCR showed transcripts of SLC transporter in T24 and T24-CR cells. C The protein expression of SLC7A6 and SLC43A2 from patient derived CD8 + T cells and BCa cells. D Relative expression of MAT2A, circARHGAP10, SLC7A6 and CD8A in TMA. The contingency correlating the staining intensity of relative marker was analyzed with chi-squared test. E SLC7A6 knockdown efficiency in T24-CR cells. F Effects of expression of H3K79me2/STAT5 in CD8 + T cell with culturing with supernatants from shSLC7A6-T24CR cells. G Effects of shSLC7A6-T24CR cells affected function of CD8 + T cell. Data are presented as the mean ± SD. *** p < 0.001 versus Complete Medium. H SLC7A6 expression in BCa tissue samples from recurrence after 6 months ( n = 9) or recurrence in 6 months ( n = 8). I Relative SLC7A6 expression from paired BCa tissue samples from patients received cisplatin based chemotherapy ( n = 5). J Combination of BCH with anti-PD-L1 treatment on MB49-CR cell xenograft model (6 mice were enrolled in each treatment group). K Tumor volumes were presented as mean ± SD. *** p < 0.001 versus LV-NC group. L Tumor weight were presented as mean ± SD. *** p < 0.001, * p < 0.05 versus LV-NC group.
Figure Legend Snippet: A Effects of BCH or MeAIB on supernatant of T24CR cells affected function of CD8 + T cell. Data are presented as the mean ± SD. *** p < 0.001, ** p < 0.01 versus Sup. B RT-qPCR showed transcripts of SLC transporter in T24 and T24-CR cells. C The protein expression of SLC7A6 and SLC43A2 from patient derived CD8 + T cells and BCa cells. D Relative expression of MAT2A, circARHGAP10, SLC7A6 and CD8A in TMA. The contingency correlating the staining intensity of relative marker was analyzed with chi-squared test. E SLC7A6 knockdown efficiency in T24-CR cells. F Effects of expression of H3K79me2/STAT5 in CD8 + T cell with culturing with supernatants from shSLC7A6-T24CR cells. G Effects of shSLC7A6-T24CR cells affected function of CD8 + T cell. Data are presented as the mean ± SD. *** p < 0.001 versus Complete Medium. H SLC7A6 expression in BCa tissue samples from recurrence after 6 months ( n = 9) or recurrence in 6 months ( n = 8). I Relative SLC7A6 expression from paired BCa tissue samples from patients received cisplatin based chemotherapy ( n = 5). J Combination of BCH with anti-PD-L1 treatment on MB49-CR cell xenograft model (6 mice were enrolled in each treatment group). K Tumor volumes were presented as mean ± SD. *** p < 0.001 versus LV-NC group. L Tumor weight were presented as mean ± SD. *** p < 0.001, * p < 0.05 versus LV-NC group.

Techniques Used: Quantitative RT-PCR, Expressing, Derivative Assay, Staining, Marker, Cell Culture

A 1×107 MB49-CR-luc-LV-NC or MB49-CR-luc-LV-circ cells were injected subcutaneously into four-week-old C57BL/6 male mice treated with control or MR diets with or without 180 mg/kg BCH under 6 mg/kg cisplatin treatment (6 mice were enrolled in each treatment group). B , C Relative tumor volume was calculated after mice were sacrificed with 6 weeks of treatment. D Tumor weight were measured separately in each group. Data are presented as the mean ± SD. *** p < 0.001 versus LV-NC group. E IHC analysis of the expression of CD8, Ki-67, MAT2A and CD44 in cisplatin resistant BCa cell xenograft model. F Expression of IL-2, IFN-γ, granzyme B and PD-1 in CD8 + T cells from mice xenograft. Data are presented as the mean ± SD. *** p < 0.001, ** p < 0.01, * p < 0.05 versus LV-NC. G Schematic diagram of the pivotal role of methionine metabolism in cisplatin resistant BCa microenvironments.
Figure Legend Snippet: A 1×107 MB49-CR-luc-LV-NC or MB49-CR-luc-LV-circ cells were injected subcutaneously into four-week-old C57BL/6 male mice treated with control or MR diets with or without 180 mg/kg BCH under 6 mg/kg cisplatin treatment (6 mice were enrolled in each treatment group). B , C Relative tumor volume was calculated after mice were sacrificed with 6 weeks of treatment. D Tumor weight were measured separately in each group. Data are presented as the mean ± SD. *** p < 0.001 versus LV-NC group. E IHC analysis of the expression of CD8, Ki-67, MAT2A and CD44 in cisplatin resistant BCa cell xenograft model. F Expression of IL-2, IFN-γ, granzyme B and PD-1 in CD8 + T cells from mice xenograft. Data are presented as the mean ± SD. *** p < 0.001, ** p < 0.01, * p < 0.05 versus LV-NC. G Schematic diagram of the pivotal role of methionine metabolism in cisplatin resistant BCa microenvironments.

Techniques Used: Injection, Expressing


Structured Review

Proteintech mat2a
Human primer sequences.
Mat2a, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mat2a/product/Proteintech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mat2a - by Bioz Stars, 2024-04
93/100 stars

Images

1) Product Images from "Oxidative Stress Aggravates Apoptosis of Nucleus Pulposus Cells through m 6 A Modification of MAT2A Pre-mRNA by METTL16"

Article Title: Oxidative Stress Aggravates Apoptosis of Nucleus Pulposus Cells through m 6 A Modification of MAT2A Pre-mRNA by METTL16

Journal: Oxidative Medicine and Cellular Longevity

doi: 10.1155/2022/4036274

Human primer sequences.
Figure Legend Snippet: Human primer sequences.

Techniques Used:

MAT2A decreased and METTL16 increased in human degenerative nucleus pulposus. (a) The expression of MAT2A in human NP tissues was analyzed by qRT-PCR. (b) The expression of METTL16 in human NP tissues analyzed by qRT-PCR. (c) Immunofluorescence assay for the expression of MAT2A and METTL16 proteins in human NP tissues. ∗∗∗ p < 0.001.
Figure Legend Snippet: MAT2A decreased and METTL16 increased in human degenerative nucleus pulposus. (a) The expression of MAT2A in human NP tissues was analyzed by qRT-PCR. (b) The expression of METTL16 in human NP tissues analyzed by qRT-PCR. (c) Immunofluorescence assay for the expression of MAT2A and METTL16 proteins in human NP tissues. ∗∗∗ p < 0.001.

Techniques Used: Expressing, Quantitative RT-PCR, Immunofluorescence

MAT2A was downregulated while METTL16 was upregulated when human nucleus pulposus cell was under oxidative stress. Human NPCs were exposed to TBHP (100 μ m, 4 hours). (a) NPC identification by characterization of their cell surface marker CD24 (b) The expression of MAT2A mRNA was detected by qRT-PCR. (c) The expression of METTL16 mRNA was detected by qRT-PCR. (d) Western blotting for the expression of MAT2A and METTL16 proteins. (e) Immunofluorescence assay for the expression of MAT2A and METTL16 proteins. (f) SAM concentration in the cell culture medium supernatant was detected by ELISA. n = 3 replicates per group, ∗∗∗ p < 0.001, 0.001 ≤ ∗∗ p < 0.05, ∗ p < 0.05.
Figure Legend Snippet: MAT2A was downregulated while METTL16 was upregulated when human nucleus pulposus cell was under oxidative stress. Human NPCs were exposed to TBHP (100 μ m, 4 hours). (a) NPC identification by characterization of their cell surface marker CD24 (b) The expression of MAT2A mRNA was detected by qRT-PCR. (c) The expression of METTL16 mRNA was detected by qRT-PCR. (d) Western blotting for the expression of MAT2A and METTL16 proteins. (e) Immunofluorescence assay for the expression of MAT2A and METTL16 proteins. (f) SAM concentration in the cell culture medium supernatant was detected by ELISA. n = 3 replicates per group, ∗∗∗ p < 0.001, 0.001 ≤ ∗∗ p < 0.05, ∗ p < 0.05.

Techniques Used: Marker, Expressing, Quantitative RT-PCR, Western Blot, Immunofluorescence, Concentration Assay, Cell Culture, Enzyme-linked Immunosorbent Assay

Downregulation of MAT2A or upregulation of METTL16 in NPCs promotes apoptosis. (a) Successful downregulation of MAT2A mRNA or upregulation of METTL16 mRNA in human NPCs confirmed by qRT-PCR. (b) SAM concentration in the supernatants of cell culture medium measured by ELISA method. (c) Apoptosis assayed by TUNEL staining significantly increased in the NPCs with MAT2A siRNA transfection. (d) Western blot analysis for the protein markers of apoptosis in cells with MAT2A siRNA transfection. (e) Flow cytometric analysis for the apoptosis of NPCs treated by MAT2A siRNA. (f) TUNEL staining for the apoptosis of NPCs with METTL16 overexpression. (g) The expression levels of Bcl2, Bax, and cleaved-Caspase3 detected by Western blot in cells with METTL16 overexpression. (h) Flow cytometric analysis of apoptosis in NPCs with METTL16 overexpression. n = 3 replicates per group, ∗∗∗ p < 0.001, 0.001 ≤ ∗∗ p < 0.05, ∗ p < 0.05.
Figure Legend Snippet: Downregulation of MAT2A or upregulation of METTL16 in NPCs promotes apoptosis. (a) Successful downregulation of MAT2A mRNA or upregulation of METTL16 mRNA in human NPCs confirmed by qRT-PCR. (b) SAM concentration in the supernatants of cell culture medium measured by ELISA method. (c) Apoptosis assayed by TUNEL staining significantly increased in the NPCs with MAT2A siRNA transfection. (d) Western blot analysis for the protein markers of apoptosis in cells with MAT2A siRNA transfection. (e) Flow cytometric analysis for the apoptosis of NPCs treated by MAT2A siRNA. (f) TUNEL staining for the apoptosis of NPCs with METTL16 overexpression. (g) The expression levels of Bcl2, Bax, and cleaved-Caspase3 detected by Western blot in cells with METTL16 overexpression. (h) Flow cytometric analysis of apoptosis in NPCs with METTL16 overexpression. n = 3 replicates per group, ∗∗∗ p < 0.001, 0.001 ≤ ∗∗ p < 0.05, ∗ p < 0.05.

Techniques Used: Quantitative RT-PCR, Concentration Assay, Cell Culture, Enzyme-linked Immunosorbent Assay, TUNEL Assay, Staining, Transfection, Western Blot, Over Expression, Expressing

The effect of METTL16 on MAT2A. (a) In degenerative human NP tissues, qRT-PCR showed that exon1-3 and harpin1 reflecting the total of MAT2A pre-mRNA and MAT2A mRNA decreased, while intron8 reflecting MAT2A pre-mRNA increased. (b) In the NPCs stimulated by TBHP, qRT-PCR showed exon1-3 and harpin1 decreased while intron8 increased. (c) In the NPCs with METTL16 overexpression, qRT-PCR showed MAT2A exon1-3 and harpin1 decreased, while intron8 increased. (d) Immunofluorescence showed that the expression of MAT2A protein decreased when METTL16 was upregulated in NPCs. The expression of MAT2A was higher in the NPCs with unsuccessful overexpression of METTL16 (white arrow) than in the cells with successful overexpression of METTL16 (black arrow). (e) MAT2A and METTL16 proteins detected by Western blot in cells with METTL16 overexpression. n = 3 replicates per group, ∗∗∗ p < 0.001, 0.001 ≤ ∗∗ p < 0.05, ∗ p < 0.05.
Figure Legend Snippet: The effect of METTL16 on MAT2A. (a) In degenerative human NP tissues, qRT-PCR showed that exon1-3 and harpin1 reflecting the total of MAT2A pre-mRNA and MAT2A mRNA decreased, while intron8 reflecting MAT2A pre-mRNA increased. (b) In the NPCs stimulated by TBHP, qRT-PCR showed exon1-3 and harpin1 decreased while intron8 increased. (c) In the NPCs with METTL16 overexpression, qRT-PCR showed MAT2A exon1-3 and harpin1 decreased, while intron8 increased. (d) Immunofluorescence showed that the expression of MAT2A protein decreased when METTL16 was upregulated in NPCs. The expression of MAT2A was higher in the NPCs with unsuccessful overexpression of METTL16 (white arrow) than in the cells with successful overexpression of METTL16 (black arrow). (e) MAT2A and METTL16 proteins detected by Western blot in cells with METTL16 overexpression. n = 3 replicates per group, ∗∗∗ p < 0.001, 0.001 ≤ ∗∗ p < 0.05, ∗ p < 0.05.

Techniques Used: Quantitative RT-PCR, Over Expression, Immunofluorescence, Expressing, Western Blot

Increased apoptosis of human NPCs under oxidative stress can be rescued by reducing the expression of METTL16 in the cells. Human NPCs were transfected with METTL16 siRNA. (a) qRT-PCR analysis for transfection efficiency. (b) The cells were then stimulated with TBHP (100 μ m, 4 hours). And qRT-PCR was employed to detect different fragments of MAT2A pre-mRNA. (c) MAT2A protein detected by immunofluorescence. (d) The protein expression levels of METTL16, MAT2A, Bcl2, Bax, and cleaved Caspase3 were detected by Western blot in cells with METTL16 siRNA and under TBHP stimulation. (e) Flow cytometry for the apoptosis rate of the NPCs. n = 3 replicates per group, ∗∗∗ p < 0.001, 0.001 ≤ ∗∗ p < 0.05, ∗ p < 0.05.
Figure Legend Snippet: Increased apoptosis of human NPCs under oxidative stress can be rescued by reducing the expression of METTL16 in the cells. Human NPCs were transfected with METTL16 siRNA. (a) qRT-PCR analysis for transfection efficiency. (b) The cells were then stimulated with TBHP (100 μ m, 4 hours). And qRT-PCR was employed to detect different fragments of MAT2A pre-mRNA. (c) MAT2A protein detected by immunofluorescence. (d) The protein expression levels of METTL16, MAT2A, Bcl2, Bax, and cleaved Caspase3 were detected by Western blot in cells with METTL16 siRNA and under TBHP stimulation. (e) Flow cytometry for the apoptosis rate of the NPCs. n = 3 replicates per group, ∗∗∗ p < 0.001, 0.001 ≤ ∗∗ p < 0.05, ∗ p < 0.05.

Techniques Used: Expressing, Transfection, Quantitative RT-PCR, Immunofluorescence, Western Blot, Flow Cytometry

In vivo studies. (a) Verification of the disc degeneration animal model. X-ray examination revealed a significant decrease of the height of the punctured disc. Safranine O/fast green staining showed that the punctured NP tissue became more disorganized, and fewer cells could be seen. The histological grading score was significantly higher in the punctured disc. Immunohistochemical analysis demonstrated less MAT2A protein and more METTL16 protein levels in the punctured NP tissues. (b) Degenerative changes in the discs injected with METTL16 overexpression lentivirus. X-ray examination revealed significant decrease of the height of the discs. Safranine O/fast green staining showed that the NP tissue became more disorganized, and fewer cells could be seen. The histological grading score was also significantly higher. Immunohistochemical analysis demonstrated less MAT2A protein and more METTL16 protein levels in the NP tissues. (c) Degenerative changes in the discs injected with cycloleucine. X-ray examination revealed significant decrease of the height of the discs. Safranine O/fast green staining showed that the NP tissue became more disorganized, and fewer cells could be seen. The histological grading score was also significantly higher. n = 3 replicates per group, ∗∗∗ p < 0.001, 0.001 ≤ ∗∗ p < 0.05, ∗ p < 0.05.
Figure Legend Snippet: In vivo studies. (a) Verification of the disc degeneration animal model. X-ray examination revealed a significant decrease of the height of the punctured disc. Safranine O/fast green staining showed that the punctured NP tissue became more disorganized, and fewer cells could be seen. The histological grading score was significantly higher in the punctured disc. Immunohistochemical analysis demonstrated less MAT2A protein and more METTL16 protein levels in the punctured NP tissues. (b) Degenerative changes in the discs injected with METTL16 overexpression lentivirus. X-ray examination revealed significant decrease of the height of the discs. Safranine O/fast green staining showed that the NP tissue became more disorganized, and fewer cells could be seen. The histological grading score was also significantly higher. Immunohistochemical analysis demonstrated less MAT2A protein and more METTL16 protein levels in the NP tissues. (c) Degenerative changes in the discs injected with cycloleucine. X-ray examination revealed significant decrease of the height of the discs. Safranine O/fast green staining showed that the NP tissue became more disorganized, and fewer cells could be seen. The histological grading score was also significantly higher. n = 3 replicates per group, ∗∗∗ p < 0.001, 0.001 ≤ ∗∗ p < 0.05, ∗ p < 0.05.

Techniques Used: In Vivo, Animal Model, Staining, Immunohistochemical staining, Injection, Over Expression


Structured Review

Proteintech anti mat2a
Primer sequences used for quantitative real-time PCR.
Anti Mat2a, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti mat2a/product/Proteintech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti mat2a - by Bioz Stars, 2024-04
93/100 stars

Images

1) Product Images from "M 6 A RNA Methylation Mediates NOD1/NF-kB Signaling Activation in the Liver of Piglets Challenged with Lipopolysaccharide"

Article Title: M 6 A RNA Methylation Mediates NOD1/NF-kB Signaling Activation in the Liver of Piglets Challenged with Lipopolysaccharide

Journal: Antioxidants

doi: 10.3390/antiox11101954

Primer sequences used for quantitative real-time PCR.
Figure Legend Snippet: Primer sequences used for quantitative real-time PCR.

Techniques Used: Sequencing

The content of ROS, HIF-1α, and MAT2A in the liver of piglets. ( A ) ROS contents were detected by using dihydroethidium (DHE)-stained liver cryosections in piglets (40× magnification). Scale bar = 50 μm. ImageJ analysis was used to quantify the number of ROS-positive cells. ( B , C ) The HIF-1α and MAT2A mRNA levels were examined in the total liver by RT-qPCR. ( D ) HIF-1α and MAT2A protein levels were assessed by western blot, quantified using ImageJ analysis, and normalized to β-actin. n = 4−6 per group. Results are showed as means with SEM represented by a vertical bar. * represents significant differences between the CON group and the LPS group; * p < 0.05, ** p < 0.01, *** p < 0.001.
Figure Legend Snippet: The content of ROS, HIF-1α, and MAT2A in the liver of piglets. ( A ) ROS contents were detected by using dihydroethidium (DHE)-stained liver cryosections in piglets (40× magnification). Scale bar = 50 μm. ImageJ analysis was used to quantify the number of ROS-positive cells. ( B , C ) The HIF-1α and MAT2A mRNA levels were examined in the total liver by RT-qPCR. ( D ) HIF-1α and MAT2A protein levels were assessed by western blot, quantified using ImageJ analysis, and normalized to β-actin. n = 4−6 per group. Results are showed as means with SEM represented by a vertical bar. * represents significant differences between the CON group and the LPS group; * p < 0.05, ** p < 0.01, *** p < 0.001.

Techniques Used: Staining, Quantitative RT-PCR, Western Blot

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    Proteintech mat2a
    <t>MAT2A</t> was decreased and MAT2B was increased in the hippocampus post-rmTBI. MAT2A and MATB were assessed via capillary electrophoresis. (A) MAT2A (catalytic subunit) had a trending decrease at 7 days (blue bar) relative to SHAM (black bar). (B) MAT2B (regulatory subunit) was significantly enhanced 7 days (blue bar) post-rmTBI relative to SHAM (black bar). (C–D) MAT2A and MAT2B were unchanged in the cerebral cortex post-rmTBI. Results were expressed as mean ± SEM. *p ≤ 0.05, evaluated by one-way ANOVA with Tukey’s post-hoc test, (n = 3–6).
    Mat2a, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mat2a/product/Proteintech
    Average 86 stars, based on 1 article reviews
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    mat2a - by Bioz Stars, 2024-04
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    86
    Proteintech rabbit anti mat2a antibody
    <t>MAT2A</t> was decreased and MAT2B was increased in the hippocampus post-rmTBI. MAT2A and MATB were assessed via capillary electrophoresis. (A) MAT2A (catalytic subunit) had a trending decrease at 7 days (blue bar) relative to SHAM (black bar). (B) MAT2B (regulatory subunit) was significantly enhanced 7 days (blue bar) post-rmTBI relative to SHAM (black bar). (C–D) MAT2A and MAT2B were unchanged in the cerebral cortex post-rmTBI. Results were expressed as mean ± SEM. *p ≤ 0.05, evaluated by one-way ANOVA with Tukey’s post-hoc test, (n = 3–6).
    Rabbit Anti Mat2a Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mat2a antibody/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti mat2a antibody - by Bioz Stars, 2024-04
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    86
    Proteintech antibodies to mat2a
    Primer sequences used for real-time PCR.
    Antibodies To Mat2a, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies to mat2a/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies to mat2a - by Bioz Stars, 2024-04
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    86
    Proteintech anti mat2a
    Primer sequences used for real-time PCR.
    Anti Mat2a, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mat2a/product/Proteintech
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    anti mat2a - by Bioz Stars, 2024-04
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    86
    Proteintech mat2a antibody
    Primer sequences used for real-time PCR.
    Mat2a Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mat2a antibody/product/Proteintech
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    MAT2A was decreased and MAT2B was increased in the hippocampus post-rmTBI. MAT2A and MATB were assessed via capillary electrophoresis. (A) MAT2A (catalytic subunit) had a trending decrease at 7 days (blue bar) relative to SHAM (black bar). (B) MAT2B (regulatory subunit) was significantly enhanced 7 days (blue bar) post-rmTBI relative to SHAM (black bar). (C–D) MAT2A and MAT2B were unchanged in the cerebral cortex post-rmTBI. Results were expressed as mean ± SEM. *p ≤ 0.05, evaluated by one-way ANOVA with Tukey’s post-hoc test, (n = 3–6).

    Journal: Neurochemistry international

    Article Title: A role for protein arginine methyltransferase 7 in repetitive and mild traumatic brain injury

    doi: 10.1016/j.neuint.2023.105524

    Figure Lengend Snippet: MAT2A was decreased and MAT2B was increased in the hippocampus post-rmTBI. MAT2A and MATB were assessed via capillary electrophoresis. (A) MAT2A (catalytic subunit) had a trending decrease at 7 days (blue bar) relative to SHAM (black bar). (B) MAT2B (regulatory subunit) was significantly enhanced 7 days (blue bar) post-rmTBI relative to SHAM (black bar). (C–D) MAT2A and MAT2B were unchanged in the cerebral cortex post-rmTBI. Results were expressed as mean ± SEM. *p ≤ 0.05, evaluated by one-way ANOVA with Tukey’s post-hoc test, (n = 3–6).

    Article Snippet: All antibodies were diluted using an antibody diluent (ProteinSimple, Bio-techne, Minneapolis, MN) as follows: iba1 (1:50; GeneTex GTX100042), PRMT7 (1:100; D1K6R), Monomethylarginine (MMA 1:50; 8015S), (Cell Signaling Technology, Danvers, MA), Bax (1:50; R&D Systems AF820), MAT2A (1:500; Novus NB110-94158), MAT2B (1:40; ProteinTech 15952-I-AP).

    Techniques: Electrophoresis

    MAT2A inhibitor reduced PRMT7 protein expression and MMA production. PF-9366 allosteric inhibitor was used (25 μM) to inhibit MAT2A in HT22 cells for 24 hrs. Inhibition of MAT2A significantly decreased (A) PRMT7 protein expression (B) decreased mono-methylarginine (MMa) production (C) increased MAT2A protein expression and significantly decreased (D) MAT2B protein expression. Results were expressed as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 evaluated by student’s t-test. (n = 3).

    Journal: Neurochemistry international

    Article Title: A role for protein arginine methyltransferase 7 in repetitive and mild traumatic brain injury

    doi: 10.1016/j.neuint.2023.105524

    Figure Lengend Snippet: MAT2A inhibitor reduced PRMT7 protein expression and MMA production. PF-9366 allosteric inhibitor was used (25 μM) to inhibit MAT2A in HT22 cells for 24 hrs. Inhibition of MAT2A significantly decreased (A) PRMT7 protein expression (B) decreased mono-methylarginine (MMa) production (C) increased MAT2A protein expression and significantly decreased (D) MAT2B protein expression. Results were expressed as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 evaluated by student’s t-test. (n = 3).

    Article Snippet: All antibodies were diluted using an antibody diluent (ProteinSimple, Bio-techne, Minneapolis, MN) as follows: iba1 (1:50; GeneTex GTX100042), PRMT7 (1:100; D1K6R), Monomethylarginine (MMA 1:50; 8015S), (Cell Signaling Technology, Danvers, MA), Bax (1:50; R&D Systems AF820), MAT2A (1:500; Novus NB110-94158), MAT2B (1:40; ProteinTech 15952-I-AP).

    Techniques: Expressing, Inhibition

    Primer sequences used for real-time PCR.

    Journal: Journal of Oral Microbiology

    Article Title: MAT2A inhibition suppresses inflammation in Porphyromonas gingivalis -infected human gingival fibroblasts

    doi: 10.1080/20002297.2023.2292375

    Figure Lengend Snippet: Primer sequences used for real-time PCR.

    Article Snippet: Proteins at 10 μL per lane were separated by 4–20% SDS-PAGE (Beyotime, China) and then transferred onto PVDF membranes (Merck, Germany), which were blocked with 5% bovine albumin and then incubated with primary antibodies to MAT2A (1:1000; 55309–1-AP; Proteintech, China), IL-1β (1:1000; BJ11258993; Bioss, China), TNF-α (1:400; GB11188; Servicebio, China), IL-6 (1:1000; 21865–1-AP; Proteintech, China), MCP-1 (1:1500; ab25124; Abcam, USA), P65 (1:1000; 8242S; CST, Germany), Erk (1:1500; GB11560; Servicebio, China), P38 (1:1000;8690S; CST, Germany), JNK (1:1000; AF6318; Affinity, USA), p-P65 (1:1000; 3033S; CST, Germany), p-Erk (1:1000; AF1015; Affinity, USA), p-P38 (1:1000; 4511S; CST, Germany), p-JNK (1:1000;4668S; CST, Germany) and β-ACTIN (1:50000; 66009-l-Ig; Proteintech, China).

    Techniques: Sequencing

    MAT2A expression was upregulated in inflamed gingival tissues.

    Journal: Journal of Oral Microbiology

    Article Title: MAT2A inhibition suppresses inflammation in Porphyromonas gingivalis -infected human gingival fibroblasts

    doi: 10.1080/20002297.2023.2292375

    Figure Lengend Snippet: MAT2A expression was upregulated in inflamed gingival tissues.

    Article Snippet: Proteins at 10 μL per lane were separated by 4–20% SDS-PAGE (Beyotime, China) and then transferred onto PVDF membranes (Merck, Germany), which were blocked with 5% bovine albumin and then incubated with primary antibodies to MAT2A (1:1000; 55309–1-AP; Proteintech, China), IL-1β (1:1000; BJ11258993; Bioss, China), TNF-α (1:400; GB11188; Servicebio, China), IL-6 (1:1000; 21865–1-AP; Proteintech, China), MCP-1 (1:1500; ab25124; Abcam, USA), P65 (1:1000; 8242S; CST, Germany), Erk (1:1500; GB11560; Servicebio, China), P38 (1:1000;8690S; CST, Germany), JNK (1:1000; AF6318; Affinity, USA), p-P65 (1:1000; 3033S; CST, Germany), p-Erk (1:1000; AF1015; Affinity, USA), p-P38 (1:1000; 4511S; CST, Germany), p-JNK (1:1000;4668S; CST, Germany) and β-ACTIN (1:50000; 66009-l-Ig; Proteintech, China).

    Techniques: Expressing

    MAT2A inhibition decreased P. gingivalis -induced inflammatory responses in hGfs.

    Journal: Journal of Oral Microbiology

    Article Title: MAT2A inhibition suppresses inflammation in Porphyromonas gingivalis -infected human gingival fibroblasts

    doi: 10.1080/20002297.2023.2292375

    Figure Lengend Snippet: MAT2A inhibition decreased P. gingivalis -induced inflammatory responses in hGfs.

    Article Snippet: Proteins at 10 μL per lane were separated by 4–20% SDS-PAGE (Beyotime, China) and then transferred onto PVDF membranes (Merck, Germany), which were blocked with 5% bovine albumin and then incubated with primary antibodies to MAT2A (1:1000; 55309–1-AP; Proteintech, China), IL-1β (1:1000; BJ11258993; Bioss, China), TNF-α (1:400; GB11188; Servicebio, China), IL-6 (1:1000; 21865–1-AP; Proteintech, China), MCP-1 (1:1500; ab25124; Abcam, USA), P65 (1:1000; 8242S; CST, Germany), Erk (1:1500; GB11560; Servicebio, China), P38 (1:1000;8690S; CST, Germany), JNK (1:1000; AF6318; Affinity, USA), p-P65 (1:1000; 3033S; CST, Germany), p-Erk (1:1000; AF1015; Affinity, USA), p-P38 (1:1000; 4511S; CST, Germany), p-JNK (1:1000;4668S; CST, Germany) and β-ACTIN (1:50000; 66009-l-Ig; Proteintech, China).

    Techniques: Inhibition

    MAT2A-knockdown reduced inflammation in P. gingivalis -infected hGFs.

    Journal: Journal of Oral Microbiology

    Article Title: MAT2A inhibition suppresses inflammation in Porphyromonas gingivalis -infected human gingival fibroblasts

    doi: 10.1080/20002297.2023.2292375

    Figure Lengend Snippet: MAT2A-knockdown reduced inflammation in P. gingivalis -infected hGFs.

    Article Snippet: Proteins at 10 μL per lane were separated by 4–20% SDS-PAGE (Beyotime, China) and then transferred onto PVDF membranes (Merck, Germany), which were blocked with 5% bovine albumin and then incubated with primary antibodies to MAT2A (1:1000; 55309–1-AP; Proteintech, China), IL-1β (1:1000; BJ11258993; Bioss, China), TNF-α (1:400; GB11188; Servicebio, China), IL-6 (1:1000; 21865–1-AP; Proteintech, China), MCP-1 (1:1500; ab25124; Abcam, USA), P65 (1:1000; 8242S; CST, Germany), Erk (1:1500; GB11560; Servicebio, China), P38 (1:1000;8690S; CST, Germany), JNK (1:1000; AF6318; Affinity, USA), p-P65 (1:1000; 3033S; CST, Germany), p-Erk (1:1000; AF1015; Affinity, USA), p-P38 (1:1000; 4511S; CST, Germany), p-JNK (1:1000;4668S; CST, Germany) and β-ACTIN (1:50000; 66009-l-Ig; Proteintech, China).

    Techniques: Infection

    NF-κB/MAPK pathway was involved in the enhanced MAT2A expression.

    Journal: Journal of Oral Microbiology

    Article Title: MAT2A inhibition suppresses inflammation in Porphyromonas gingivalis -infected human gingival fibroblasts

    doi: 10.1080/20002297.2023.2292375

    Figure Lengend Snippet: NF-κB/MAPK pathway was involved in the enhanced MAT2A expression.

    Article Snippet: Proteins at 10 μL per lane were separated by 4–20% SDS-PAGE (Beyotime, China) and then transferred onto PVDF membranes (Merck, Germany), which were blocked with 5% bovine albumin and then incubated with primary antibodies to MAT2A (1:1000; 55309–1-AP; Proteintech, China), IL-1β (1:1000; BJ11258993; Bioss, China), TNF-α (1:400; GB11188; Servicebio, China), IL-6 (1:1000; 21865–1-AP; Proteintech, China), MCP-1 (1:1500; ab25124; Abcam, USA), P65 (1:1000; 8242S; CST, Germany), Erk (1:1500; GB11560; Servicebio, China), P38 (1:1000;8690S; CST, Germany), JNK (1:1000; AF6318; Affinity, USA), p-P65 (1:1000; 3033S; CST, Germany), p-Erk (1:1000; AF1015; Affinity, USA), p-P38 (1:1000; 4511S; CST, Germany), p-JNK (1:1000;4668S; CST, Germany) and β-ACTIN (1:50000; 66009-l-Ig; Proteintech, China).

    Techniques: Expressing

    Primer sequences used for real-time PCR.

    Journal: Journal of Oral Microbiology

    Article Title: MAT2A inhibition suppresses inflammation in Porphyromonas gingivalis -infected human gingival fibroblasts

    doi: 10.1080/20002297.2023.2292375

    Figure Lengend Snippet: Primer sequences used for real-time PCR.

    Article Snippet: The sections were incubated with anti-MAT2A (1:200; 55309–1-AP; Proteintech, China) overnight at 4°C.

    Techniques: Sequencing

    MAT2A expression was upregulated in inflamed gingival tissues.

    Journal: Journal of Oral Microbiology

    Article Title: MAT2A inhibition suppresses inflammation in Porphyromonas gingivalis -infected human gingival fibroblasts

    doi: 10.1080/20002297.2023.2292375

    Figure Lengend Snippet: MAT2A expression was upregulated in inflamed gingival tissues.

    Article Snippet: The sections were incubated with anti-MAT2A (1:200; 55309–1-AP; Proteintech, China) overnight at 4°C.

    Techniques: Expressing

    MAT2A inhibition decreased P. gingivalis -induced inflammatory responses in hGfs.

    Journal: Journal of Oral Microbiology

    Article Title: MAT2A inhibition suppresses inflammation in Porphyromonas gingivalis -infected human gingival fibroblasts

    doi: 10.1080/20002297.2023.2292375

    Figure Lengend Snippet: MAT2A inhibition decreased P. gingivalis -induced inflammatory responses in hGfs.

    Article Snippet: The sections were incubated with anti-MAT2A (1:200; 55309–1-AP; Proteintech, China) overnight at 4°C.

    Techniques: Inhibition

    MAT2A-knockdown reduced inflammation in P. gingivalis -infected hGFs.

    Journal: Journal of Oral Microbiology

    Article Title: MAT2A inhibition suppresses inflammation in Porphyromonas gingivalis -infected human gingival fibroblasts

    doi: 10.1080/20002297.2023.2292375

    Figure Lengend Snippet: MAT2A-knockdown reduced inflammation in P. gingivalis -infected hGFs.

    Article Snippet: The sections were incubated with anti-MAT2A (1:200; 55309–1-AP; Proteintech, China) overnight at 4°C.

    Techniques: Infection

    NF-κB/MAPK pathway was involved in the enhanced MAT2A expression.

    Journal: Journal of Oral Microbiology

    Article Title: MAT2A inhibition suppresses inflammation in Porphyromonas gingivalis -infected human gingival fibroblasts

    doi: 10.1080/20002297.2023.2292375

    Figure Lengend Snippet: NF-κB/MAPK pathway was involved in the enhanced MAT2A expression.

    Article Snippet: The sections were incubated with anti-MAT2A (1:200; 55309–1-AP; Proteintech, China) overnight at 4°C.

    Techniques: Expressing