anti machr m3  (Alomone Labs)


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    Alomone Labs anti machr m3
    Expression of key components in the <t>mAChR</t> signaling pathway. The expression levels of (A) mAChR M2, and (B) <t>mAChR</t> M3 and their respective downstream proteins were determined by (a-c) western blot and (d) immunofluorescence assays. Band intensities were evaluated relative to the intensity of the actin bands. Data are presented as mean ± standard deviation of three replicates (n=5-6 rats per treatment group). * P
    Anti Machr M3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti machr m3/product/Alomone Labs
    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    anti machr m3 - by Bioz Stars, 2022-10
    94/100 stars

    Images

    1) Product Images from "Spicatoside A in red Liriope platyphylla displays a laxative effect in a constipation rat model via regulating mAChRs and ER stress signaling"

    Article Title: Spicatoside A in red Liriope platyphylla displays a laxative effect in a constipation rat model via regulating mAChRs and ER stress signaling

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2018.3960

    Expression of key components in the mAChR signaling pathway. The expression levels of (A) mAChR M2, and (B) mAChR M3 and their respective downstream proteins were determined by (a-c) western blot and (d) immunofluorescence assays. Band intensities were evaluated relative to the intensity of the actin bands. Data are presented as mean ± standard deviation of three replicates (n=5-6 rats per treatment group). * P
    Figure Legend Snippet: Expression of key components in the mAChR signaling pathway. The expression levels of (A) mAChR M2, and (B) mAChR M3 and their respective downstream proteins were determined by (a-c) western blot and (d) immunofluorescence assays. Band intensities were evaluated relative to the intensity of the actin bands. Data are presented as mean ± standard deviation of three replicates (n=5-6 rats per treatment group). * P

    Techniques Used: Expressing, Western Blot, Immunofluorescence, Standard Deviation

    2) Product Images from "Spicatoside A in red Liriope platyphylla displays a laxative effect in a constipation rat model via regulating mAChRs and ER stress signaling"

    Article Title: Spicatoside A in red Liriope platyphylla displays a laxative effect in a constipation rat model via regulating mAChRs and ER stress signaling

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2018.3960

    Expression of key components in the mAChR signaling pathway. The expression levels of (A) mAChR M2, and (B) mAChR M3 and their respective downstream proteins were determined by (a-c) western blot and (d) immunofluorescence assays. Band intensities were evaluated relative to the intensity of the actin bands. Data are presented as mean ± standard deviation of three replicates (n=5-6 rats per treatment group).  * P
    Figure Legend Snippet: Expression of key components in the mAChR signaling pathway. The expression levels of (A) mAChR M2, and (B) mAChR M3 and their respective downstream proteins were determined by (a-c) western blot and (d) immunofluorescence assays. Band intensities were evaluated relative to the intensity of the actin bands. Data are presented as mean ± standard deviation of three replicates (n=5-6 rats per treatment group). * P

    Techniques Used: Expressing, Western Blot, Immunofluorescence, Standard Deviation

    3) Product Images from "Antioxidant activity and laxative effects of tannin-enriched extract of Ecklonia cava in loperamide-induced constipation of SD rats"

    Article Title: Antioxidant activity and laxative effects of tannin-enriched extract of Ecklonia cava in loperamide-induced constipation of SD rats

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0246363

    Expressions of mAChRs and key mediators within their downstream signaling pathway in Lop+TEE treated constipation rats. Expression levels of mAChRs and key mediators, including mAChR M2, mAChR M3, Gα, PKC, p-PKC, PI3K and p-PI3K, in the mAChR M2 and M3 signaling pathway were measured by Western blot analysis using specific primary antibodies and HRP-labeled anti-rabbit IgG antibody. After the intensity of each band was determined using an imaging densitometer, relative levels of the seven proteins were calculated based on the intensity of actin. Four to six rats per group were used in the preparation of the total tissue homogenate, and Western blot analyses were assayed in duplicate in each sample. The data are reported as the mean ± SD. *, p
    Figure Legend Snippet: Expressions of mAChRs and key mediators within their downstream signaling pathway in Lop+TEE treated constipation rats. Expression levels of mAChRs and key mediators, including mAChR M2, mAChR M3, Gα, PKC, p-PKC, PI3K and p-PI3K, in the mAChR M2 and M3 signaling pathway were measured by Western blot analysis using specific primary antibodies and HRP-labeled anti-rabbit IgG antibody. After the intensity of each band was determined using an imaging densitometer, relative levels of the seven proteins were calculated based on the intensity of actin. Four to six rats per group were used in the preparation of the total tissue homogenate, and Western blot analyses were assayed in duplicate in each sample. The data are reported as the mean ± SD. *, p

    Techniques Used: Expressing, Western Blot, Labeling, Imaging

    4) Product Images from "Spicatoside A in red Liriope platyphylla displays a laxative effect in a constipation rat model via regulating mAChRs and ER stress signaling"

    Article Title: Spicatoside A in red Liriope platyphylla displays a laxative effect in a constipation rat model via regulating mAChRs and ER stress signaling

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2018.3960

    Expression of key components in the mAChR signaling pathway. The expression levels of (A) mAChR M2, and (B) mAChR M3 and their respective downstream proteins were determined by (a-c) western blot and (d) immunofluorescence assays. Band intensities were evaluated relative to the intensity of the actin bands. Data are presented as mean ± standard deviation of three replicates (n=5-6 rats per treatment group). * P
    Figure Legend Snippet: Expression of key components in the mAChR signaling pathway. The expression levels of (A) mAChR M2, and (B) mAChR M3 and their respective downstream proteins were determined by (a-c) western blot and (d) immunofluorescence assays. Band intensities were evaluated relative to the intensity of the actin bands. Data are presented as mean ± standard deviation of three replicates (n=5-6 rats per treatment group). * P

    Techniques Used: Expressing, Western Blot, Immunofluorescence, Standard Deviation

    5) Product Images from "Molecular Characterization of Constipation Disease as Novel Phenotypes in CRISPR-Cas9-Generated Leptin Knockout Mice with Obesity"

    Article Title: Molecular Characterization of Constipation Disease as Novel Phenotypes in CRISPR-Cas9-Generated Leptin Knockout Mice with Obesity

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms21249464

    Expression of mAChRs and key mediators within their downstream signaling pathway. ( a ) Expression levels of mAChR M2 and mAChR M3 were measured by Western blot analysis using the specific primary antibodies and HRP-labeled anti-rabbit IgG antibody; ( b ) Expression levels of the key mediators, including Gα, PKC, p-PKC, PI3K and p-PI3K, in the mAChR M2 and M3 signaling pathways, were measured by Western blot analysis using the specific primary antibodies and HRP-labeled anti-rabbit IgG antibody. After determining the intensity of each band using an imaging densitometer, the relative levels of the four proteins were calculated based on the intensity of β-actin. Three to five mice per group were used to prepare the total tissue homogenate, and Western blot analyses were assayed in duplicate in each sample. The data are reported as the mean ± SD. *,  p
    Figure Legend Snippet: Expression of mAChRs and key mediators within their downstream signaling pathway. ( a ) Expression levels of mAChR M2 and mAChR M3 were measured by Western blot analysis using the specific primary antibodies and HRP-labeled anti-rabbit IgG antibody; ( b ) Expression levels of the key mediators, including Gα, PKC, p-PKC, PI3K and p-PI3K, in the mAChR M2 and M3 signaling pathways, were measured by Western blot analysis using the specific primary antibodies and HRP-labeled anti-rabbit IgG antibody. After determining the intensity of each band using an imaging densitometer, the relative levels of the four proteins were calculated based on the intensity of β-actin. Three to five mice per group were used to prepare the total tissue homogenate, and Western blot analyses were assayed in duplicate in each sample. The data are reported as the mean ± SD. *, p

    Techniques Used: Expressing, Western Blot, Labeling, Imaging, Mouse Assay

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    Alomone Labs chrm3
    Repression of P2rx1 by progressive vascular endothelial damage. ( a – c ) RQ-PCR ( a ) and western blot ( b , c ) analysis of the expressions of the cholinergic receptor muscarinic 2 ( Chrm2 ), cholinergic receptor muscarinic 3 ( <t>Chrm3</t> ), and purinergic receptor P2X 1 ( P2rx1 ) genes in the bladders of rats in the indicated groups. Expression levels of the indicated transcripts are presented as % Gapdh . For western blot analysis, β-actin was used as a loading control, and the expression levels of the indicated proteins were normalized to the β-actin value. ( d , e ) Representative images ( d ) and quantification analysis ( e ) of the immunohistochemical staining for the P2rx1 protein (original magnification ×200, scale bar = 200 μm). All quantification results are presented as mean ± SEM. *p
    Chrm3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chrm3/product/Alomone Labs
    Average 94 stars, based on 7 article reviews
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    chrm3 - by Bioz Stars, 2022-10
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    Repression of P2rx1 by progressive vascular endothelial damage. ( a – c ) RQ-PCR ( a ) and western blot ( b , c ) analysis of the expressions of the cholinergic receptor muscarinic 2 ( Chrm2 ), cholinergic receptor muscarinic 3 ( Chrm3 ), and purinergic receptor P2X 1 ( P2rx1 ) genes in the bladders of rats in the indicated groups. Expression levels of the indicated transcripts are presented as % Gapdh . For western blot analysis, β-actin was used as a loading control, and the expression levels of the indicated proteins were normalized to the β-actin value. ( d , e ) Representative images ( d ) and quantification analysis ( e ) of the immunohistochemical staining for the P2rx1 protein (original magnification ×200, scale bar = 200 μm). All quantification results are presented as mean ± SEM. *p

    Journal: Scientific Reports

    Article Title: Induction of detrusor underactivity by extensive vascular endothelial damages of iliac arteries in a rat model and its pathophysiology in the genetic levels

    doi: 10.1038/s41598-019-52811-4

    Figure Lengend Snippet: Repression of P2rx1 by progressive vascular endothelial damage. ( a – c ) RQ-PCR ( a ) and western blot ( b , c ) analysis of the expressions of the cholinergic receptor muscarinic 2 ( Chrm2 ), cholinergic receptor muscarinic 3 ( Chrm3 ), and purinergic receptor P2X 1 ( P2rx1 ) genes in the bladders of rats in the indicated groups. Expression levels of the indicated transcripts are presented as % Gapdh . For western blot analysis, β-actin was used as a loading control, and the expression levels of the indicated proteins were normalized to the β-actin value. ( d , e ) Representative images ( d ) and quantification analysis ( e ) of the immunohistochemical staining for the P2rx1 protein (original magnification ×200, scale bar = 200 μm). All quantification results are presented as mean ± SEM. *p

    Article Snippet: Protein levels were assessed from 50 μg of extracts separated using 10% SDS-PAGE gels by probing with antibodies specific to P2rx1 (APR-001, Alomone Labs), Chrm2 (AMR-002, Alomone Labs,), Chrm3 (AMR-006, Alomone Labs, Israel), α-SMA (ab5694, Rabbit, Abcam), vimentin (sc-6260, Santa Cruz Biotechnology Inc., CA), and β-actin (Sigma-Aldrich).

    Techniques: Polymerase Chain Reaction, Western Blot, Expressing, Immunohistochemistry, Staining

    Correlation between YAP/TAZ, MRTF/SRF, and muscarinic receptors in the human GI tract and urinary bladder . ( A ) For the table, RNA sequencing data from GTExPortal.org was used to correlate mRNA levels of YAP1 , TEAD3 , and WWTR1 vs MYOCD , MRTFA , SRF , CHRM2 , and CHRM3 . Spearman Rho values for the indicated correlations are given for 5 sections of the GI tract and the urinary bladder. All Spearman Rho values in bold have a P value less than .0001. Examples of correlations in the transverse colon are highlighted in gray. ( B ) Examples of correlations in the transverse colon are plotted. White lines are best-fit linear regression lines. eso GEJ, esophagus gastroesophageal junction; colon s., colon sigmoid; colon t., colon transverse; ter. ileum, terminal ileum; TPM, transcripts per million.

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: Inducible Deletion of YAP and TAZ in Adult Mouse Smooth Muscle Causes Rapid and Lethal Colonic Pseudo-Obstruction

    doi: 10.1016/j.jcmgh.2020.09.014

    Figure Lengend Snippet: Correlation between YAP/TAZ, MRTF/SRF, and muscarinic receptors in the human GI tract and urinary bladder . ( A ) For the table, RNA sequencing data from GTExPortal.org was used to correlate mRNA levels of YAP1 , TEAD3 , and WWTR1 vs MYOCD , MRTFA , SRF , CHRM2 , and CHRM3 . Spearman Rho values for the indicated correlations are given for 5 sections of the GI tract and the urinary bladder. All Spearman Rho values in bold have a P value less than .0001. Examples of correlations in the transverse colon are highlighted in gray. ( B ) Examples of correlations in the transverse colon are plotted. White lines are best-fit linear regression lines. eso GEJ, esophagus gastroesophageal junction; colon s., colon sigmoid; colon t., colon transverse; ter. ileum, terminal ileum; TPM, transcripts per million.

    Article Snippet: Specific proteins were detected using commercially available primary antibodies: YAP/TAZ (cat. 8418, 1:1000; Cell Signaling Technology), α-actin (cat. A5228, 1:1000; Sigma-Aldrich), smooth muscle myosin heavy chain (cat. ab53219, 1:1000; abcam), Chrm2 (cat. AMR-002, 1:1000; alomone labs, Jerusalem), Chrm3 (cat. AMR-006, 1:200; alomone labs), SRF (cat. 5147S, 1:1000; Cell Signaling Technology), and myosin light chain (3672S, 1:1000; Cell Signaling Technology).

    Techniques: RNA Sequencing Assay

    Expression of key components in the mAChR signaling pathway. The expression levels of (A) mAChR M2, and (B) mAChR M3 and their respective downstream proteins were determined by (a-c) western blot and (d) immunofluorescence assays. Band intensities were evaluated relative to the intensity of the actin bands. Data are presented as mean ± standard deviation of three replicates (n=5-6 rats per treatment group). * P

    Journal: International Journal of Molecular Medicine

    Article Title: Spicatoside A in red Liriope platyphylla displays a laxative effect in a constipation rat model via regulating mAChRs and ER stress signaling

    doi: 10.3892/ijmm.2018.3960

    Figure Lengend Snippet: Expression of key components in the mAChR signaling pathway. The expression levels of (A) mAChR M2, and (B) mAChR M3 and their respective downstream proteins were determined by (a-c) western blot and (d) immunofluorescence assays. Band intensities were evaluated relative to the intensity of the actin bands. Data are presented as mean ± standard deviation of three replicates (n=5-6 rats per treatment group). * P

    Article Snippet: The pretreated sections were then incubated with anti-mAChR M2 (1:200, cat. no. AMR-002; Alomone Labs) or anti-mAChR M3 (1:200, cat. no. AMR-006; Alomone Labs) antibodies diluted 1:1,000 in blocking buffer.

    Techniques: Expressing, Western Blot, Immunofluorescence, Standard Deviation

    Expression of key components in the mAChR signaling pathway. The expression levels of (A) mAChR M2, and (B) mAChR M3 and their respective downstream proteins were determined by (a-c) western blot and (d) immunofluorescence assays. Band intensities were evaluated relative to the intensity of the actin bands. Data are presented as mean ± standard deviation of three replicates (n=5-6 rats per treatment group).  * P

    Journal: International Journal of Molecular Medicine

    Article Title: Spicatoside A in red Liriope platyphylla displays a laxative effect in a constipation rat model via regulating mAChRs and ER stress signaling

    doi: 10.3892/ijmm.2018.3960

    Figure Lengend Snippet: Expression of key components in the mAChR signaling pathway. The expression levels of (A) mAChR M2, and (B) mAChR M3 and their respective downstream proteins were determined by (a-c) western blot and (d) immunofluorescence assays. Band intensities were evaluated relative to the intensity of the actin bands. Data are presented as mean ± standard deviation of three replicates (n=5-6 rats per treatment group). * P

    Article Snippet: The pretreated sections were then incubated with anti-mAChR M2 (1:200, cat. no. AMR-002; Alomone Labs) or anti-mAChR M3 (1:200, cat. no. AMR-006; Alomone Labs) antibodies diluted 1:1,000 in blocking buffer.

    Techniques: Expressing, Western Blot, Immunofluorescence, Standard Deviation