kv7 2  (Alomone Labs)


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    Structured Review

    Alomone Labs kv7 2
    Long-term drinking reduced SUMOylation levels of <t>Kv7.2</t> in the NAc. (a) Schematic depicting locations of the SUMOylation consensus sequences in rat Kv7.2 (orange circle and arrow indicate point of SUMO attachment). (b) Representative western blot images showing the expression of SUMOylated Kv7.2 in fractionated NAc tissue. (c) 72 hr withdrawal from alcohol decreased expression of SUMOylated Kv7.2 in the DSM but not the DRM fraction. (d) However, IAA exposure reduced the ratio of SUMOylated Kv7.2 to total Kv7.2 in the DRM fraction (* p
    Kv7 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kv7 2/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kv7 2 - by Bioz Stars, 2022-05
    93/100 stars

    Images

    1) Product Images from "Kv7 Channels in the Nucleus Accumbens are altered by Chronic Drinking and are Targets for Reducing Alcohol Consumption"

    Article Title: Kv7 Channels in the Nucleus Accumbens are altered by Chronic Drinking and are Targets for Reducing Alcohol Consumption

    Journal: Addiction biology

    doi: 10.1111/adb.12279

    Long-term drinking reduced SUMOylation levels of Kv7.2 in the NAc. (a) Schematic depicting locations of the SUMOylation consensus sequences in rat Kv7.2 (orange circle and arrow indicate point of SUMO attachment). (b) Representative western blot images showing the expression of SUMOylated Kv7.2 in fractionated NAc tissue. (c) 72 hr withdrawal from alcohol decreased expression of SUMOylated Kv7.2 in the DSM but not the DRM fraction. (d) However, IAA exposure reduced the ratio of SUMOylated Kv7.2 to total Kv7.2 in the DRM fraction (* p
    Figure Legend Snippet: Long-term drinking reduced SUMOylation levels of Kv7.2 in the NAc. (a) Schematic depicting locations of the SUMOylation consensus sequences in rat Kv7.2 (orange circle and arrow indicate point of SUMO attachment). (b) Representative western blot images showing the expression of SUMOylated Kv7.2 in fractionated NAc tissue. (c) 72 hr withdrawal from alcohol decreased expression of SUMOylated Kv7.2 in the DSM but not the DRM fraction. (d) However, IAA exposure reduced the ratio of SUMOylated Kv7.2 to total Kv7.2 in the DRM fraction (* p

    Techniques Used: Western Blot, Expressing

    Long-term drinking altered Kv7.2 surface trafficking in the NAc. (a) Representative western blot images showing the relative expression patterns of Kv7.2, AKAP150, and SGK1 in the DRM, IC, and DSM fractions in tissue targeting the NAc core. (b) Representative western blot images of Kv7.2, AKAP150, and SGK1 expression in the NAc of alcohol naïve and IAA rats after 72 hr withdrawal from alcohol. (c) Kv7.2 expression is increased in the DRM of IAA rats and decreased in the DSM. There is no effect of alcohol exposure on the expression of (d) AKAP150 in the DRM or (e) SGK1 in the IC fraction (* p
    Figure Legend Snippet: Long-term drinking altered Kv7.2 surface trafficking in the NAc. (a) Representative western blot images showing the relative expression patterns of Kv7.2, AKAP150, and SGK1 in the DRM, IC, and DSM fractions in tissue targeting the NAc core. (b) Representative western blot images of Kv7.2, AKAP150, and SGK1 expression in the NAc of alcohol naïve and IAA rats after 72 hr withdrawal from alcohol. (c) Kv7.2 expression is increased in the DRM of IAA rats and decreased in the DSM. There is no effect of alcohol exposure on the expression of (d) AKAP150 in the DRM or (e) SGK1 in the IC fraction (* p

    Techniques Used: Western Blot, Expressing

    2) Product Images from "G9a Is Essential for Epigenetic Silencing of K+ Channel Genes in Acute-to-Chronic Pain Transition"

    Article Title: G9a Is Essential for Epigenetic Silencing of K+ Channel Genes in Acute-to-Chronic Pain Transition

    Journal: Nature neuroscience

    doi: 10.1038/nn.4165

    Inhibition of G9a activity normalizes K + channel gene expression in the DRG diminished by nerve injury ( a-d) Effects of intrathecal treatments with vehicle (n = 10), SAHA (50 μg, n = 9), UNC0638 (10 μg, n = 8), GSK503 (5 μg, n = 10), SAHA plus GSK503 (n = 8), SAHA plus UNC0638 (n = 9) or UNC0638 plus GSK503 (n = 8) on the mRNA levels of Kcna4 ( a ), Kcnd2 ( b ), Kcnq2 ( c ), and Kcnma1 ( d ) in the DRG obtained from SNL rats 28 days after surgery. Data from sham control rats were plotted as the control (n = 6 rats). ( e,f ) Effects of nerve injury and UNC0638 on the protein levels of Kv1.4, Kv4.2, Kv7.2 and BKα1 in the L5 and L6 DRG (n = 6 rats in each group). ( g,h ) Effect of G9a-specific siRNA on the G9a and H3K9me2 protein levels in the DRG obtained from SNL rats 24 h after the last injection (n = 5 in each group). ( i,j ) Effects of G9a-specific siRNA on the mRNA levels of G9a, Ezh2, Kcna4, Kcnd2, Kcnq2 and Kcnma1 in the DRG obtained from SNL ( i ) and sham control ( j ) rats 24 h after the last injection (n = 10 in each group). Data are presented as mean ± s.e.m. Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s post hoc tests ( a-d ), one-way ANOVA ( f,h,i ), or Mann-Whitney test ( j ). * P
    Figure Legend Snippet: Inhibition of G9a activity normalizes K + channel gene expression in the DRG diminished by nerve injury ( a-d) Effects of intrathecal treatments with vehicle (n = 10), SAHA (50 μg, n = 9), UNC0638 (10 μg, n = 8), GSK503 (5 μg, n = 10), SAHA plus GSK503 (n = 8), SAHA plus UNC0638 (n = 9) or UNC0638 plus GSK503 (n = 8) on the mRNA levels of Kcna4 ( a ), Kcnd2 ( b ), Kcnq2 ( c ), and Kcnma1 ( d ) in the DRG obtained from SNL rats 28 days after surgery. Data from sham control rats were plotted as the control (n = 6 rats). ( e,f ) Effects of nerve injury and UNC0638 on the protein levels of Kv1.4, Kv4.2, Kv7.2 and BKα1 in the L5 and L6 DRG (n = 6 rats in each group). ( g,h ) Effect of G9a-specific siRNA on the G9a and H3K9me2 protein levels in the DRG obtained from SNL rats 24 h after the last injection (n = 5 in each group). ( i,j ) Effects of G9a-specific siRNA on the mRNA levels of G9a, Ezh2, Kcna4, Kcnd2, Kcnq2 and Kcnma1 in the DRG obtained from SNL ( i ) and sham control ( j ) rats 24 h after the last injection (n = 10 in each group). Data are presented as mean ± s.e.m. Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s post hoc tests ( a-d ), one-way ANOVA ( f,h,i ), or Mann-Whitney test ( j ). * P

    Techniques Used: Inhibition, Activity Assay, Expressing, Injection, MANN-WHITNEY

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    Alomone Labs anti kv7 2
    NP1 interacts and colocalizes with <t>Kv7.2</t> at presynaptic terminals of excitatory synapses and axonal growth cones. A – D , Representative Western blots of immunoprecipitation eluates separated in 6% Tris-Glycine (for high molecular weight proteins) and 4–12% Bis-Tris gels (for low molecular weight proteins). TL, Total protein lysate; Syx, syntaxin. A , Both native Kv7.2 and syntaxin 1A, but not Kv7.3, coprecipitate with NP1 in total brain extracts. B , Both native Kv7.2 and NP1 coprecipitate with syntaxin in total brain extracts. C , D , Recombinant NP1 coprecipitates Kv7.2, but not syntaxin or Kv7.3, in 293T cells transfected with NP1, 5Myc-Kv7.2, 2HA-Kv7.3, and syntaxin 1A cDNAs. Kv7.2 and Kv7.3 were immunoprecipitated with antibodies against their respective Myc and HA tags. E , F , Immunofluorescence studies and confocal microscopy were performed in high-density ( E ) or low-density ( F ) isolated cortical neurons. E , Top, Confocal sections of 0.772 μm in the z -plane showing immunofluorescence of NP1, VGLUT1, Kv7.2, and negative control (omitting primary antibodies). Bottom, Colocalization (in white) of the excitatory presynaptic marker VGLUT1 (blue) with NP1 (green) and Kv7.2 (red) is shown in a single section with the corresponding orthogonal views of the stack of confocal sections. White arrows indicate sites of colocalization. F , NP1 (green) and KV7.2 (magenta) immunofluorescence and DIC images of an isolated cortical cultured neuron (1×) with its corresponding axonal growth cone highlighted in a white square box, shown in a confocal section of 0.772 μm in the z -plane at higher (5×) magnification. The negative control for primary antibodies is shown in another growth cone on the right. The image in the bottom is the merge of NP1 and Kv7.2 immunofluorescence images in the single confocal section obtained at 5× showing colocalization (white) of NP1 and Kv7.2 in the growth cone, with the corresponding orthogonal views from its respective stack of confocal sections. Images were acquired using restricted spectral emission wavelength ranges chosen to avoid crosstalk or bleed-through between the three different channels. Scale bar, 5 μm.
    Anti Kv7 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kv7 2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kv7 2 - by Bioz Stars, 2022-05
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    NP1 interacts and colocalizes with Kv7.2 at presynaptic terminals of excitatory synapses and axonal growth cones. A – D , Representative Western blots of immunoprecipitation eluates separated in 6% Tris-Glycine (for high molecular weight proteins) and 4–12% Bis-Tris gels (for low molecular weight proteins). TL, Total protein lysate; Syx, syntaxin. A , Both native Kv7.2 and syntaxin 1A, but not Kv7.3, coprecipitate with NP1 in total brain extracts. B , Both native Kv7.2 and NP1 coprecipitate with syntaxin in total brain extracts. C , D , Recombinant NP1 coprecipitates Kv7.2, but not syntaxin or Kv7.3, in 293T cells transfected with NP1, 5Myc-Kv7.2, 2HA-Kv7.3, and syntaxin 1A cDNAs. Kv7.2 and Kv7.3 were immunoprecipitated with antibodies against their respective Myc and HA tags. E , F , Immunofluorescence studies and confocal microscopy were performed in high-density ( E ) or low-density ( F ) isolated cortical neurons. E , Top, Confocal sections of 0.772 μm in the z -plane showing immunofluorescence of NP1, VGLUT1, Kv7.2, and negative control (omitting primary antibodies). Bottom, Colocalization (in white) of the excitatory presynaptic marker VGLUT1 (blue) with NP1 (green) and Kv7.2 (red) is shown in a single section with the corresponding orthogonal views of the stack of confocal sections. White arrows indicate sites of colocalization. F , NP1 (green) and KV7.2 (magenta) immunofluorescence and DIC images of an isolated cortical cultured neuron (1×) with its corresponding axonal growth cone highlighted in a white square box, shown in a confocal section of 0.772 μm in the z -plane at higher (5×) magnification. The negative control for primary antibodies is shown in another growth cone on the right. The image in the bottom is the merge of NP1 and Kv7.2 immunofluorescence images in the single confocal section obtained at 5× showing colocalization (white) of NP1 and Kv7.2 in the growth cone, with the corresponding orthogonal views from its respective stack of confocal sections. Images were acquired using restricted spectral emission wavelength ranges chosen to avoid crosstalk or bleed-through between the three different channels. Scale bar, 5 μm.

    Journal: The Journal of Neuroscience

    Article Title: Neuronal Pentraxin 1 Negatively Regulates Excitatory Synapse Density and Synaptic Plasticity

    doi: 10.1523/JNEUROSCI.2548-14.2015

    Figure Lengend Snippet: NP1 interacts and colocalizes with Kv7.2 at presynaptic terminals of excitatory synapses and axonal growth cones. A – D , Representative Western blots of immunoprecipitation eluates separated in 6% Tris-Glycine (for high molecular weight proteins) and 4–12% Bis-Tris gels (for low molecular weight proteins). TL, Total protein lysate; Syx, syntaxin. A , Both native Kv7.2 and syntaxin 1A, but not Kv7.3, coprecipitate with NP1 in total brain extracts. B , Both native Kv7.2 and NP1 coprecipitate with syntaxin in total brain extracts. C , D , Recombinant NP1 coprecipitates Kv7.2, but not syntaxin or Kv7.3, in 293T cells transfected with NP1, 5Myc-Kv7.2, 2HA-Kv7.3, and syntaxin 1A cDNAs. Kv7.2 and Kv7.3 were immunoprecipitated with antibodies against their respective Myc and HA tags. E , F , Immunofluorescence studies and confocal microscopy were performed in high-density ( E ) or low-density ( F ) isolated cortical neurons. E , Top, Confocal sections of 0.772 μm in the z -plane showing immunofluorescence of NP1, VGLUT1, Kv7.2, and negative control (omitting primary antibodies). Bottom, Colocalization (in white) of the excitatory presynaptic marker VGLUT1 (blue) with NP1 (green) and Kv7.2 (red) is shown in a single section with the corresponding orthogonal views of the stack of confocal sections. White arrows indicate sites of colocalization. F , NP1 (green) and KV7.2 (magenta) immunofluorescence and DIC images of an isolated cortical cultured neuron (1×) with its corresponding axonal growth cone highlighted in a white square box, shown in a confocal section of 0.772 μm in the z -plane at higher (5×) magnification. The negative control for primary antibodies is shown in another growth cone on the right. The image in the bottom is the merge of NP1 and Kv7.2 immunofluorescence images in the single confocal section obtained at 5× showing colocalization (white) of NP1 and Kv7.2 in the growth cone, with the corresponding orthogonal views from its respective stack of confocal sections. Images were acquired using restricted spectral emission wavelength ranges chosen to avoid crosstalk or bleed-through between the three different channels. Scale bar, 5 μm.

    Article Snippet: Incubation with primary antibodies was performed overnight at 4°C with the following antibodies, diluted in 3% BSA/PBS or 1% NGS/PBS (NP1, VGLUT1, and Kv7.2): mouse monoclonal anti-PSD95 (1:50; Abcam), anti-gephyrin (1:100; Synaptic Systems), anti-NP1 (1:50, BD Transduction), rabbit polyclonal anti-synaptophysin (1:100; Synaptic Systems), anti-Kv7.2 (1:100; Alomone Labs), and guinea pig anti-VGLUT1 (1:200; Millipore Bioscience Research Reagents).

    Techniques: Western Blot, Immunoprecipitation, Molecular Weight, Recombinant, Transfection, Immunofluorescence, Confocal Microscopy, Isolation, Negative Control, Marker, Cell Culture

    Knockdown of NP1 reduces Kv7.2 surface expression and M-current. A , B , The proportion of Kv7.2 at the neuronal surface was determined by biotinylation (30 min at 4°C) of all surface proteins in cultured cortical neurons. The ratio of the surface over the total amount of receptor was determined by densitometric analysis and expressed as a percentage of control values. Only samples of biotinylated proteins lacking immunoreactivity to actin, which indicates contamination with intracellular proteins, were used for analyses. A , Representative Western blots of Kv7.2, Na-KATPase, and actin from total (T) and biotinylated (B) proteins. B , Quantitative analysis of the effect of gain and loss of NP1 on the surface/total levels of the Kv7.2 subunit of the voltage-gated Kv7/M K + channels. Values are mean ± SEM of shRdm ( n = 11), shNP1 ( n = 11), and pWPI-NP1 ( n = 5), from at least three independent experiments; * p

    Journal: The Journal of Neuroscience

    Article Title: Neuronal Pentraxin 1 Negatively Regulates Excitatory Synapse Density and Synaptic Plasticity

    doi: 10.1523/JNEUROSCI.2548-14.2015

    Figure Lengend Snippet: Knockdown of NP1 reduces Kv7.2 surface expression and M-current. A , B , The proportion of Kv7.2 at the neuronal surface was determined by biotinylation (30 min at 4°C) of all surface proteins in cultured cortical neurons. The ratio of the surface over the total amount of receptor was determined by densitometric analysis and expressed as a percentage of control values. Only samples of biotinylated proteins lacking immunoreactivity to actin, which indicates contamination with intracellular proteins, were used for analyses. A , Representative Western blots of Kv7.2, Na-KATPase, and actin from total (T) and biotinylated (B) proteins. B , Quantitative analysis of the effect of gain and loss of NP1 on the surface/total levels of the Kv7.2 subunit of the voltage-gated Kv7/M K + channels. Values are mean ± SEM of shRdm ( n = 11), shNP1 ( n = 11), and pWPI-NP1 ( n = 5), from at least three independent experiments; * p

    Article Snippet: Incubation with primary antibodies was performed overnight at 4°C with the following antibodies, diluted in 3% BSA/PBS or 1% NGS/PBS (NP1, VGLUT1, and Kv7.2): mouse monoclonal anti-PSD95 (1:50; Abcam), anti-gephyrin (1:100; Synaptic Systems), anti-NP1 (1:50, BD Transduction), rabbit polyclonal anti-synaptophysin (1:100; Synaptic Systems), anti-Kv7.2 (1:100; Alomone Labs), and guinea pig anti-VGLUT1 (1:200; Millipore Bioscience Research Reagents).

    Techniques: Expressing, Cell Culture, Western Blot

    Long-term drinking reduced SUMOylation levels of Kv7.2 in the NAc. (a) Schematic depicting locations of the SUMOylation consensus sequences in rat Kv7.2 (orange circle and arrow indicate point of SUMO attachment). (b) Representative western blot images showing the expression of SUMOylated Kv7.2 in fractionated NAc tissue. (c) 72 hr withdrawal from alcohol decreased expression of SUMOylated Kv7.2 in the DSM but not the DRM fraction. (d) However, IAA exposure reduced the ratio of SUMOylated Kv7.2 to total Kv7.2 in the DRM fraction (* p

    Journal: Addiction biology

    Article Title: Kv7 Channels in the Nucleus Accumbens are altered by Chronic Drinking and are Targets for Reducing Alcohol Consumption

    doi: 10.1111/adb.12279

    Figure Lengend Snippet: Long-term drinking reduced SUMOylation levels of Kv7.2 in the NAc. (a) Schematic depicting locations of the SUMOylation consensus sequences in rat Kv7.2 (orange circle and arrow indicate point of SUMO attachment). (b) Representative western blot images showing the expression of SUMOylated Kv7.2 in fractionated NAc tissue. (c) 72 hr withdrawal from alcohol decreased expression of SUMOylated Kv7.2 in the DSM but not the DRM fraction. (d) However, IAA exposure reduced the ratio of SUMOylated Kv7.2 to total Kv7.2 in the DRM fraction (* p

    Article Snippet: The antibodies used in this study were Kv7.2 (#APC-050; Alomone Labs; Jerusalem, Israel), AKAP150 (#07-210; EMD Millipore; Billerica, MA), and SGK1 (#S5188; Sigma-Aldrich Co).

    Techniques: Western Blot, Expressing

    Long-term drinking altered Kv7.2 surface trafficking in the NAc. (a) Representative western blot images showing the relative expression patterns of Kv7.2, AKAP150, and SGK1 in the DRM, IC, and DSM fractions in tissue targeting the NAc core. (b) Representative western blot images of Kv7.2, AKAP150, and SGK1 expression in the NAc of alcohol naïve and IAA rats after 72 hr withdrawal from alcohol. (c) Kv7.2 expression is increased in the DRM of IAA rats and decreased in the DSM. There is no effect of alcohol exposure on the expression of (d) AKAP150 in the DRM or (e) SGK1 in the IC fraction (* p

    Journal: Addiction biology

    Article Title: Kv7 Channels in the Nucleus Accumbens are altered by Chronic Drinking and are Targets for Reducing Alcohol Consumption

    doi: 10.1111/adb.12279

    Figure Lengend Snippet: Long-term drinking altered Kv7.2 surface trafficking in the NAc. (a) Representative western blot images showing the relative expression patterns of Kv7.2, AKAP150, and SGK1 in the DRM, IC, and DSM fractions in tissue targeting the NAc core. (b) Representative western blot images of Kv7.2, AKAP150, and SGK1 expression in the NAc of alcohol naïve and IAA rats after 72 hr withdrawal from alcohol. (c) Kv7.2 expression is increased in the DRM of IAA rats and decreased in the DSM. There is no effect of alcohol exposure on the expression of (d) AKAP150 in the DRM or (e) SGK1 in the IC fraction (* p

    Article Snippet: The antibodies used in this study were Kv7.2 (#APC-050; Alomone Labs; Jerusalem, Israel), AKAP150 (#07-210; EMD Millipore; Billerica, MA), and SGK1 (#S5188; Sigma-Aldrich Co).

    Techniques: Western Blot, Expressing