anti kv2 2 (Alomone Labs)


Structured Review

Anti Kv2 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti kv2 2/product/Alomone Labs
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Control of Voltage-gated Potassium Channel Kv2.2 Expression by Pyruvate-Isocitrate Cycling Regulates Glucose-stimulated Insulin Secretion *"
Article Title: Control of Voltage-gated Potassium Channel Kv2.2 Expression by Pyruvate-Isocitrate Cycling Regulates Glucose-stimulated Insulin Secretion *
Journal: The Journal of Biological Chemistry
doi: 10.1074/jbc.M113.491654

Figure Legend Snippet: Regulation of Kv2.2 by pyruvate-isocitrate cycling and regulation of GSIS by Kv2.2. 832/13 cells were treated with siControl ( siCont ), siCIC, or siICDc for 72 h, and total RNA was isolated for qRT-PCR analyses of CIC, ICDc, Kv2.1, and Kv2.2 expression
Techniques Used: Isolation, Quantitative RT-PCR, Expressing

Figure Legend Snippet: Kv2.2 is expressed in α-, β- and δ-cells of the intact rodent islet. A–D , dissociated mouse islet cells were sorted based on expression of fluorescent proteins and tested for Kv2.2 expression by qRT-PCR analyses. A , transgenic
Techniques Used: Expressing, Quantitative RT-PCR, Transgenic Assay

Figure Legend Snippet: Kv2.2 overexpression rescues the inhibitory effects of siICDc on GSIS in primary islets. Freshly isolated rat islets were transduced with an equal dose of Ad-siControl ( siCont ) or Ad-siICDc ( siICDc ) and subsequently incubated for a total of 72 h. A , for
Techniques Used: Over Expression, Isolation, Transduction, Incubation

Figure Legend Snippet: Inhibitory effects of siICDc on GSIS are rescued by Kv2.2 overexpression in 832/13 cells. Immunoblot analyses of membrane protein fractions isolated from untreated (−) and from AdCMV-hKv2.2 (Kv2.2 OE)-treated 832/13 cells are shown. The AdCMV-hKv2.2
Techniques Used: Over Expression, Isolation

Figure Legend Snippet: Interactions of Kv2.2 and Kv2.1 regulate outward K + current in β-cells. 832/13 cells were transfected with siControl ( siCont ), siICDc, siKv2.1, or siKv2.2 duplexes in combination with a plasmid encoding GFP and cultured for 72 h prior to patch
Techniques Used: Transfection, Plasmid Preparation, Cell Culture

Figure Legend Snippet: Kv2.2 mRNA expression is reduced by chronic exposure to elevated fatty acids. 832/13 cells were cultured for a total of 3 days and exposed to a mixture of 1 m m O/P complexed to BSA in a 2:1 ratio or to BSA alone for the final 24 or 48 h as indicated.
Techniques Used: Expressing, Cell Culture