Journal: The American Journal of Pathology
Article Title: Increased Smooth Muscle Kv11.1 Channel Expression in Pulmonary Hypertension and Protective Role of Kv11.1 Channel Blocker Dofetilide
Figure Lengend Snippet: Kv11.1 channel expression gradually increases with the development of pulmonary arterial hypertension (PAH) in rats. A: Time course examination of Kv11.1 expression in PAH rats (brown; arrows ). For this experiment Fischer rats were injected with SU5416, placed in hypoxia for 3 weeks, and then maintained in normoxia for 2 weeks to obtain time points of 0, 1, 2, 3, 4, and 5 weeks after the SU5416 injection. B: Negative control for the corresponding images in A with the primary antibody omitted and only the secondary antibody used. C: Quantification of the changes in Kv11.1 channel expression [in arbitrary units (au)] with PAH development in small pulmonary arteries (PAs). The mean of the data is indicated ( red lines ). n = 10. * P
Article Snippet: Tissue sections were subjected to hematoxylin and eosin (H & E) staining and immunohistochemistry (IHC) analysis using the α-smooth muscle actin primary antibody (1:300; catalog number ab5694; Abcam, Cambridge, UK) or Kv11.1 primary antibody (1:1000; catalog number APC-016; Alomone Laboratories, Jerusalem, Israel), followed by a secondary horseradish peroxidase–conjugated antibody (catalog number K4003; Agilent, Santa Clara, CA).
Techniques: Expressing, Injection, Negative Control