Structured Review

The Jackson Laboratory anti kv1 3 kcna3 extracellular antibody
<t>Kv1.3</t> and KCa3.1 currents are expressed in D10 cells. ( A ): Representative Kv1.3 K + current in a single D10 cell recorded during a 1.5-s-long test pulse to +50 mV from a holding potential of –120 mV. The superimposed dashed line indicates the best fit single exponential with τ =252 ms. ( B ): Representative Kv1.3 K + currents from a single D10 cell in control solution, and after the equilibration of the block in the presence of 15 pM MgTx (test pulse: +50 mV). ( C ): Voltage ramps from –120 mV to +50 mV (duration: 150 ms) evoked KCa3.1 currents from a single D10 cell. Traces show the current in control solution, after the equilibration of the block in the presence of 250 nM TRAM-34, and after wash-out. The voltage range below the activation threshold of Kv1.3 channels is shown only.
Anti Kv1 3 Kcna3 Extracellular Antibody, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti kv1 3 kcna3 extracellular antibody/product/The Jackson Laboratory
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti kv1 3 kcna3 extracellular antibody - by Bioz Stars, 2022-08
86/100 stars

Images

1) Product Images from "Periodic Membrane Potential and Ca2+ Oscillations in T Cells Forming an Immune Synapse"

Article Title: Periodic Membrane Potential and Ca2+ Oscillations in T Cells Forming an Immune Synapse

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms21051568

Kv1.3 and KCa3.1 currents are expressed in D10 cells. ( A ): Representative Kv1.3 K + current in a single D10 cell recorded during a 1.5-s-long test pulse to +50 mV from a holding potential of –120 mV. The superimposed dashed line indicates the best fit single exponential with τ =252 ms. ( B ): Representative Kv1.3 K + currents from a single D10 cell in control solution, and after the equilibration of the block in the presence of 15 pM MgTx (test pulse: +50 mV). ( C ): Voltage ramps from –120 mV to +50 mV (duration: 150 ms) evoked KCa3.1 currents from a single D10 cell. Traces show the current in control solution, after the equilibration of the block in the presence of 250 nM TRAM-34, and after wash-out. The voltage range below the activation threshold of Kv1.3 channels is shown only.
Figure Legend Snippet: Kv1.3 and KCa3.1 currents are expressed in D10 cells. ( A ): Representative Kv1.3 K + current in a single D10 cell recorded during a 1.5-s-long test pulse to +50 mV from a holding potential of –120 mV. The superimposed dashed line indicates the best fit single exponential with τ =252 ms. ( B ): Representative Kv1.3 K + currents from a single D10 cell in control solution, and after the equilibration of the block in the presence of 15 pM MgTx (test pulse: +50 mV). ( C ): Voltage ramps from –120 mV to +50 mV (duration: 150 ms) evoked KCa3.1 currents from a single D10 cell. Traces show the current in control solution, after the equilibration of the block in the presence of 250 nM TRAM-34, and after wash-out. The voltage range below the activation threshold of Kv1.3 channels is shown only.

Techniques Used: Blocking Assay, Activation Assay

Recruitment of PKCθ-GFP and Kv1.3 into the IS. Representative confocal images of a D10 cell alone ( A – D ) or conjugated to a CH12 cell (E-H). Panels from left to right display: ( A , E ): GFP signal of PKCΘ (green), ( B , F ): Cy3 fluorescence of Kv1.3 signal (red), ( C , G ): merge of the PKCθ-GFP and Kv1.3 signals. ( D , H ): bright field image of the cells. Slice thickness was set to 1 μm. The image was taken 20 min after mixing and centrifuging together the two cell types. Scale bar is 10 µm.
Figure Legend Snippet: Recruitment of PKCθ-GFP and Kv1.3 into the IS. Representative confocal images of a D10 cell alone ( A – D ) or conjugated to a CH12 cell (E-H). Panels from left to right display: ( A , E ): GFP signal of PKCΘ (green), ( B , F ): Cy3 fluorescence of Kv1.3 signal (red), ( C , G ): merge of the PKCθ-GFP and Kv1.3 signals. ( D , H ): bright field image of the cells. Slice thickness was set to 1 μm. The image was taken 20 min after mixing and centrifuging together the two cell types. Scale bar is 10 µm.

Techniques Used: Fluorescence

Membrane potential oscillations are sensitive to Kv1.3 and KCa3.1 channel blockers and to extracellular calcium concentration. ( A , B ): Representative membrane potential records (current-clamp) of D10 cells conjugated to CH12 cells. MPO was by induced by depolarizing current injections (arrows) with durations indicated by the lengths of the horizontal lines. The hatched horizontal bar indicates perfusion with 50 nM ChTx ( A ) or with 250 nM TRAM-34 ( B ). ( C ): MPO amplitudes of individual cells were averaged (mean ± SEM) in control extracellular solution (Ctrl) and following the perfusion with 50 nM ChTx (ChTx). ( D ): MPO amplitudes of individual cells were averaged (mean ± SEM) in control extracellular solution (Ctrl) and following or 250 nM TRAM-34 (TRAM-34). Oblique lines connect the corresponding average amplitudes in Ctrl and in the presence of the blockers. ( E ): Effect of ChTx and TRAM-34 on the MPO amplitudes. The MPO amplitudes (Amp) were pooled before (Ctrl ChTx) and after 50 nM ChTx (ChTX) or before (Ctrl TRAM-34) and after 250 nM TRAM-34 (TRAM-34) and averaged (mean ± SEM). Asterisks indicate significant differences (* p
Figure Legend Snippet: Membrane potential oscillations are sensitive to Kv1.3 and KCa3.1 channel blockers and to extracellular calcium concentration. ( A , B ): Representative membrane potential records (current-clamp) of D10 cells conjugated to CH12 cells. MPO was by induced by depolarizing current injections (arrows) with durations indicated by the lengths of the horizontal lines. The hatched horizontal bar indicates perfusion with 50 nM ChTx ( A ) or with 250 nM TRAM-34 ( B ). ( C ): MPO amplitudes of individual cells were averaged (mean ± SEM) in control extracellular solution (Ctrl) and following the perfusion with 50 nM ChTx (ChTx). ( D ): MPO amplitudes of individual cells were averaged (mean ± SEM) in control extracellular solution (Ctrl) and following or 250 nM TRAM-34 (TRAM-34). Oblique lines connect the corresponding average amplitudes in Ctrl and in the presence of the blockers. ( E ): Effect of ChTx and TRAM-34 on the MPO amplitudes. The MPO amplitudes (Amp) were pooled before (Ctrl ChTx) and after 50 nM ChTx (ChTX) or before (Ctrl TRAM-34) and after 250 nM TRAM-34 (TRAM-34) and averaged (mean ± SEM). Asterisks indicate significant differences (* p

Techniques Used: Concentration Assay

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    The Jackson Laboratory anti kv1 3 kcna3 extracellular antibody
    <t>Kv1.3</t> and KCa3.1 currents are expressed in D10 cells. ( A ): Representative Kv1.3 K + current in a single D10 cell recorded during a 1.5-s-long test pulse to +50 mV from a holding potential of –120 mV. The superimposed dashed line indicates the best fit single exponential with τ =252 ms. ( B ): Representative Kv1.3 K + currents from a single D10 cell in control solution, and after the equilibration of the block in the presence of 15 pM MgTx (test pulse: +50 mV). ( C ): Voltage ramps from –120 mV to +50 mV (duration: 150 ms) evoked KCa3.1 currents from a single D10 cell. Traces show the current in control solution, after the equilibration of the block in the presence of 250 nM TRAM-34, and after wash-out. The voltage range below the activation threshold of Kv1.3 channels is shown only.
    Anti Kv1 3 Kcna3 Extracellular Antibody, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kv1 3 kcna3 extracellular antibody/product/The Jackson Laboratory
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kv1 3 kcna3 extracellular antibody - by Bioz Stars, 2022-08
    86/100 stars
      Buy from Supplier

    Image Search Results


    Kv1.3 and KCa3.1 currents are expressed in D10 cells. ( A ): Representative Kv1.3 K + current in a single D10 cell recorded during a 1.5-s-long test pulse to +50 mV from a holding potential of –120 mV. The superimposed dashed line indicates the best fit single exponential with τ =252 ms. ( B ): Representative Kv1.3 K + currents from a single D10 cell in control solution, and after the equilibration of the block in the presence of 15 pM MgTx (test pulse: +50 mV). ( C ): Voltage ramps from –120 mV to +50 mV (duration: 150 ms) evoked KCa3.1 currents from a single D10 cell. Traces show the current in control solution, after the equilibration of the block in the presence of 250 nM TRAM-34, and after wash-out. The voltage range below the activation threshold of Kv1.3 channels is shown only.

    Journal: International Journal of Molecular Sciences

    Article Title: Periodic Membrane Potential and Ca2+ Oscillations in T Cells Forming an Immune Synapse

    doi: 10.3390/ijms21051568

    Figure Lengend Snippet: Kv1.3 and KCa3.1 currents are expressed in D10 cells. ( A ): Representative Kv1.3 K + current in a single D10 cell recorded during a 1.5-s-long test pulse to +50 mV from a holding potential of –120 mV. The superimposed dashed line indicates the best fit single exponential with τ =252 ms. ( B ): Representative Kv1.3 K + currents from a single D10 cell in control solution, and after the equilibration of the block in the presence of 15 pM MgTx (test pulse: +50 mV). ( C ): Voltage ramps from –120 mV to +50 mV (duration: 150 ms) evoked KCa3.1 currents from a single D10 cell. Traces show the current in control solution, after the equilibration of the block in the presence of 250 nM TRAM-34, and after wash-out. The voltage range below the activation threshold of Kv1.3 channels is shown only.

    Article Snippet: The primary antibody was raised against the extracellular loop between S1 and S2 segments of the channel protein (amino acids 211–224: KDYPASTSQDSFEA(C)) (cat #: APC-101, 1:200 dilution, Alomone Labs, Jerusalem, Israel), the secondary antibody was a Cy3 conjugated donkey anti-rabbit IgG (1:500 dilution, The Jackson Laboratory, Sacramento, CA USA).

    Techniques: Blocking Assay, Activation Assay

    Recruitment of PKCθ-GFP and Kv1.3 into the IS. Representative confocal images of a D10 cell alone ( A – D ) or conjugated to a CH12 cell (E-H). Panels from left to right display: ( A , E ): GFP signal of PKCΘ (green), ( B , F ): Cy3 fluorescence of Kv1.3 signal (red), ( C , G ): merge of the PKCθ-GFP and Kv1.3 signals. ( D , H ): bright field image of the cells. Slice thickness was set to 1 μm. The image was taken 20 min after mixing and centrifuging together the two cell types. Scale bar is 10 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Periodic Membrane Potential and Ca2+ Oscillations in T Cells Forming an Immune Synapse

    doi: 10.3390/ijms21051568

    Figure Lengend Snippet: Recruitment of PKCθ-GFP and Kv1.3 into the IS. Representative confocal images of a D10 cell alone ( A – D ) or conjugated to a CH12 cell (E-H). Panels from left to right display: ( A , E ): GFP signal of PKCΘ (green), ( B , F ): Cy3 fluorescence of Kv1.3 signal (red), ( C , G ): merge of the PKCθ-GFP and Kv1.3 signals. ( D , H ): bright field image of the cells. Slice thickness was set to 1 μm. The image was taken 20 min after mixing and centrifuging together the two cell types. Scale bar is 10 µm.

    Article Snippet: The primary antibody was raised against the extracellular loop between S1 and S2 segments of the channel protein (amino acids 211–224: KDYPASTSQDSFEA(C)) (cat #: APC-101, 1:200 dilution, Alomone Labs, Jerusalem, Israel), the secondary antibody was a Cy3 conjugated donkey anti-rabbit IgG (1:500 dilution, The Jackson Laboratory, Sacramento, CA USA).

    Techniques: Fluorescence

    Membrane potential oscillations are sensitive to Kv1.3 and KCa3.1 channel blockers and to extracellular calcium concentration. ( A , B ): Representative membrane potential records (current-clamp) of D10 cells conjugated to CH12 cells. MPO was by induced by depolarizing current injections (arrows) with durations indicated by the lengths of the horizontal lines. The hatched horizontal bar indicates perfusion with 50 nM ChTx ( A ) or with 250 nM TRAM-34 ( B ). ( C ): MPO amplitudes of individual cells were averaged (mean ± SEM) in control extracellular solution (Ctrl) and following the perfusion with 50 nM ChTx (ChTx). ( D ): MPO amplitudes of individual cells were averaged (mean ± SEM) in control extracellular solution (Ctrl) and following or 250 nM TRAM-34 (TRAM-34). Oblique lines connect the corresponding average amplitudes in Ctrl and in the presence of the blockers. ( E ): Effect of ChTx and TRAM-34 on the MPO amplitudes. The MPO amplitudes (Amp) were pooled before (Ctrl ChTx) and after 50 nM ChTx (ChTX) or before (Ctrl TRAM-34) and after 250 nM TRAM-34 (TRAM-34) and averaged (mean ± SEM). Asterisks indicate significant differences (* p

    Journal: International Journal of Molecular Sciences

    Article Title: Periodic Membrane Potential and Ca2+ Oscillations in T Cells Forming an Immune Synapse

    doi: 10.3390/ijms21051568

    Figure Lengend Snippet: Membrane potential oscillations are sensitive to Kv1.3 and KCa3.1 channel blockers and to extracellular calcium concentration. ( A , B ): Representative membrane potential records (current-clamp) of D10 cells conjugated to CH12 cells. MPO was by induced by depolarizing current injections (arrows) with durations indicated by the lengths of the horizontal lines. The hatched horizontal bar indicates perfusion with 50 nM ChTx ( A ) or with 250 nM TRAM-34 ( B ). ( C ): MPO amplitudes of individual cells were averaged (mean ± SEM) in control extracellular solution (Ctrl) and following the perfusion with 50 nM ChTx (ChTx). ( D ): MPO amplitudes of individual cells were averaged (mean ± SEM) in control extracellular solution (Ctrl) and following or 250 nM TRAM-34 (TRAM-34). Oblique lines connect the corresponding average amplitudes in Ctrl and in the presence of the blockers. ( E ): Effect of ChTx and TRAM-34 on the MPO amplitudes. The MPO amplitudes (Amp) were pooled before (Ctrl ChTx) and after 50 nM ChTx (ChTX) or before (Ctrl TRAM-34) and after 250 nM TRAM-34 (TRAM-34) and averaged (mean ± SEM). Asterisks indicate significant differences (* p

    Article Snippet: The primary antibody was raised against the extracellular loop between S1 and S2 segments of the channel protein (amino acids 211–224: KDYPASTSQDSFEA(C)) (cat #: APC-101, 1:200 dilution, Alomone Labs, Jerusalem, Israel), the secondary antibody was a Cy3 conjugated donkey anti-rabbit IgG (1:500 dilution, The Jackson Laboratory, Sacramento, CA USA).

    Techniques: Concentration Assay