anti kv  (Alomone Labs)

Journal: CNS Neuroscience & Therapeutics

Article Title: Effects of Acetazolamide on Transient K+ Currents and Action Potentials in Nodose Ganglion Neurons of Adult Rats

doi: 10.1111/j.1755-5949.2010.00133.x

Figure Lengend Snippet: Example of mRNA encoding Kv 1.4 and Kv 4 family channel proteins in adult rat nodose ganglia. Gel electrophoresis of PCR products obtained from nodose ganglia. a ), A nucleotide size ladder in 100 bp increments. PCR products obtained with the primer for Kv 1.4 (119 bp, b ), Kv 4.1 (467 bp, c ), Kv 4.2 (110 bp, d ), Kv 4.3 (400 bp, e ), and GAPDH (418 bp, f ). Negative control (no reverse transcription, g ).

Article Snippet: In order to demonstrate Kv 1.4 and CA-II, Kv 4.1 and CA-II, Kv 4.2 and CA-II, and Kv 4.3 and CA-II fluorescent double stainings, sections were separately exposed for a period of 2 days to mix the first antibody solution with rabbit anti-Kv 1.4 (1:160, Alomone Labs Ltd., Jerusalem, Israel) and sheep anti-CA-II polyclonal (1:500, Biogenesis, USA), rabbit anti-Kv 4.1 (1:160, Alomone Labs Ltd., Jerusalem) and sheep anti-CA-II polyclonal (1:500, Biogenesis), rabbit anti-Kv 4.2 (1:160, Chemicon) and sheep anti-CA-II polyclonal (1:500, Biogenesis) and rabbit anti-Kv 4.3 (1:160, Alomone Labs Ltd., Jerusalem) and sheep anti-CA-II polyclonal (1:500, Biogenesis) at 4°C followed by incubation in a secondary antibody solution with Alexa® 568 goat anti-rabbit IgG (1:1000, Molecular Probes, Kv 1.4, Kv 4.1–4.3, red, USA) and donkey anti IgG sheep-FITC (1:1000, CA-II, green, Chemicon) for 24 h at room temperature, respectively.

Techniques: Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Negative Control

Journal: CNS Neuroscience & Therapeutics

Article Title: Effects of Acetazolamide on Transient K+ Currents and Action Potentials in Nodose Ganglion Neurons of Adult Rats

doi: 10.1111/j.1755-5949.2010.00133.x

Figure Lengend Snippet: Immunofluorescence staining of adult rat nodose ganglion neurons and histograms showing diameters of cells labeled with four Kv channel (Kv 1.4, Kv 4.1–4.3) and/or CA-II antibodies. Scale bars are 20 μm.

Article Snippet: In order to demonstrate Kv 1.4 and CA-II, Kv 4.1 and CA-II, Kv 4.2 and CA-II, and Kv 4.3 and CA-II fluorescent double stainings, sections were separately exposed for a period of 2 days to mix the first antibody solution with rabbit anti-Kv 1.4 (1:160, Alomone Labs Ltd., Jerusalem, Israel) and sheep anti-CA-II polyclonal (1:500, Biogenesis, USA), rabbit anti-Kv 4.1 (1:160, Alomone Labs Ltd., Jerusalem) and sheep anti-CA-II polyclonal (1:500, Biogenesis), rabbit anti-Kv 4.2 (1:160, Chemicon) and sheep anti-CA-II polyclonal (1:500, Biogenesis) and rabbit anti-Kv 4.3 (1:160, Alomone Labs Ltd., Jerusalem) and sheep anti-CA-II polyclonal (1:500, Biogenesis) at 4°C followed by incubation in a secondary antibody solution with Alexa® 568 goat anti-rabbit IgG (1:1000, Molecular Probes, Kv 1.4, Kv 4.1–4.3, red, USA) and donkey anti IgG sheep-FITC (1:1000, CA-II, green, Chemicon) for 24 h at room temperature, respectively.

Techniques: Immunofluorescence, Staining, Labeling

Journal: CNS Neuroscience & Therapeutics

Article Title: Effects of Acetazolamide on Transient K+ Currents and Action Potentials in Nodose Ganglion Neurons of Adult Rats

doi: 10.1111/j.1755-5949.2010.00133.x

Figure Lengend Snippet: Example of mRNA encoding Kv 1.4 and Kv 4 family channel proteins in adult rat nodose ganglia. Gel electrophoresis of PCR products obtained from nodose ganglia. a ), A nucleotide size ladder in 100 bp increments. PCR products obtained with the primer for Kv 1.4 (119 bp, b ), Kv 4.1 (467 bp, c ), Kv 4.2 (110 bp, d ), Kv 4.3 (400 bp, e ), and GAPDH (418 bp, f ). Negative control (no reverse transcription, g ).

Article Snippet: In order to demonstrate Kv 1.4 and CA-II, Kv 4.1 and CA-II, Kv 4.2 and CA-II, and Kv 4.3 and CA-II fluorescent double stainings, sections were separately exposed for a period of 2 days to mix the first antibody solution with rabbit anti-Kv 1.4 (1:160, Alomone Labs Ltd., Jerusalem, Israel) and sheep anti-CA-II polyclonal (1:500, Biogenesis, USA), rabbit anti-Kv 4.1 (1:160, Alomone Labs Ltd., Jerusalem) and sheep anti-CA-II polyclonal (1:500, Biogenesis), rabbit anti-Kv 4.2 (1:160, Chemicon) and sheep anti-CA-II polyclonal (1:500, Biogenesis) and rabbit anti-Kv 4.3 (1:160, Alomone Labs Ltd., Jerusalem) and sheep anti-CA-II polyclonal (1:500, Biogenesis) at 4°C followed by incubation in a secondary antibody solution with Alexa® 568 goat anti-rabbit IgG (1:1000, Molecular Probes, Kv 1.4, Kv 4.1–4.3, red, USA) and donkey anti IgG sheep-FITC (1:1000, CA-II, green, Chemicon) for 24 h at room temperature, respectively.

Techniques: Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Negative Control

Journal: The Journal of comparative neurology

Article Title: Localization of Kv1.3 channels in presynaptic terminals of brainstem auditory neurons

doi: 10.1002/cne.22393

Figure Lengend Snippet: Gradients of Kv1.3 immunoreactivity in sagittal and coronal sections of the MNTB. A. Sagittal section of MNTB immunostained for Kv 1.3. (scale bar, 20 μm). B and C. Coronal (B) and sagittal (C) sections were divided into 5 equal zones for quantification

Article Snippet: Anti Kv 1.3 (extracellular) antibody was obtained from Alomone labs.

Techniques:

Journal: The Journal of comparative neurology

Article Title: Localization of Kv1.3 channels in presynaptic terminals of brainstem auditory neurons

doi: 10.1002/cne.22393

Figure Lengend Snippet: Electron microscopic immunogold labeling of Kv 1.3 in the MNTB of Wild-type and Kv 1.3 −/− mice. A and B. Examples of sections stained for Kv1.3 using 10 nm gold particles. Particles are localized primarily to pre-synaptic structures,

Article Snippet: Anti Kv 1.3 (extracellular) antibody was obtained from Alomone labs.

Techniques: Labeling, Mouse Assay, Staining

Journal: The Journal of comparative neurology

Article Title: Localization of Kv1.3 channels in presynaptic terminals of brainstem auditory neurons

doi: 10.1002/cne.22393

Figure Lengend Snippet: Co-localization of Kv 1.3 with syntaxin, synaptophysin and synaptotagmin. Double immunofluorescence labeling of Kv1.3 with syntaxin (top panels A – E ), synaptophysin ( center panels F – J ) and synaptotagmin ( bottom panels K – O ). Kv 1.3

Article Snippet: Anti Kv 1.3 (extracellular) antibody was obtained from Alomone labs.

Techniques: Immunofluorescence, Labeling

Journal: CNS Neuroscience & Therapeutics

Article Title: Effects of Acetazolamide on Transient K+ Currents and Action Potentials in Nodose Ganglion Neurons of Adult Rats

doi: 10.1111/j.1755-5949.2010.00133.x

Figure Lengend Snippet: Example of mRNA encoding Kv 1.4 and Kv 4 family channel proteins in adult rat nodose ganglia. Gel electrophoresis of PCR products obtained from nodose ganglia. a ), A nucleotide size ladder in 100 bp increments. PCR products obtained with the primer for Kv 1.4 (119 bp, b ), Kv 4.1 (467 bp, c ), Kv 4.2 (110 bp, d ), Kv 4.3 (400 bp, e ), and GAPDH (418 bp, f ). Negative control (no reverse transcription, g ).

Article Snippet: In order to demonstrate Kv 1.4 and CA-II, Kv 4.1 and CA-II, Kv 4.2 and CA-II, and Kv 4.3 and CA-II fluorescent double stainings, sections were separately exposed for a period of 2 days to mix the first antibody solution with rabbit anti-Kv 1.4 (1:160, Alomone Labs Ltd., Jerusalem, Israel) and sheep anti-CA-II polyclonal (1:500, Biogenesis, USA), rabbit anti-Kv 4.1 (1:160, Alomone Labs Ltd., Jerusalem) and sheep anti-CA-II polyclonal (1:500, Biogenesis), rabbit anti-Kv 4.2 (1:160, Chemicon) and sheep anti-CA-II polyclonal (1:500, Biogenesis) and rabbit anti-Kv 4.3 (1:160, Alomone Labs Ltd., Jerusalem) and sheep anti-CA-II polyclonal (1:500, Biogenesis) at 4°C followed by incubation in a secondary antibody solution with Alexa® 568 goat anti-rabbit IgG (1:1000, Molecular Probes, Kv 1.4, Kv 4.1–4.3, red, USA) and donkey anti IgG sheep-FITC (1:1000, CA-II, green, Chemicon) for 24 h at room temperature, respectively.

Techniques: Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Negative Control