krt6  (Proteintech)

Journal: bioRxiv

Article Title: Single-cell atlas of patient-derived cervical organoids uncovers epithelial immune heterogeneity and intercellular crosstalk during Chlamydia infection

doi: 10.1101/2025.04.13.648603

Figure Lengend Snippet: a-b) UMAP of tissue-derived squamous (a) and columnar (b) epithelial subclusters; cells colored by cluster annotation. c) IHC images of human ectocervix (left panel) and endocervix (right panel) tissues (i) and organoids (ii) stained with CDH1 (green), MUC21(red), MUC5B (gray); and CDH1 (green), PAX8 (magenta), Acetylated tubulin (yellow); KRT5 (green), KRT6 (red), KRT8 (gray). Nuclei were stained with DAPI (blue). d) UMAP projection of integrated epithelial cells from organoids (O-) and tissue (T-) datasets, showing annotated subtypes based on transcriptional identity. e) Directed graph network showing four distinct communities based on transcriptional similarity between organoid-derived (pink) and tissue-derived (blue) epithelial subclusters; each node represents a cell cluster, and arrows indicate the direction of transcriptional similarity. f) Heatmap visualization of Pearson correlation matrix between squamous and columnar epithelial subclusters derived from organoids and tissue. g) Violin plot showing normalized gene expression levels of selected transcription factors (TFs) across ecto- and endocervical epithelial subtypes. h) Heatmap depicting the most variable TF activity across squamous and columnar epithelial subclusters; color bar indicates activity levels ranging from high (deep pink) to low (blue).

Article Snippet: The following primary antibodies were used for immunofluorescence: mouse anti-acetylated tubulin-Alexa-647 (Santa Cruz Biotechnology, sc-23950-AF647), mouse anti-E-cadherin-Alexa-488 (BD Biosciences, 560061), mouse anti-E-cadherin (BD Biosciences, 610181), rabbit anti-KRT5-Alexa488 (Abcam, ab193894 mouse anti-MUC5B (Abcam, ab77995), rabbit anti-MUC21 (ProteinAtlas, HPA052028), rabbit anti-KRT8 (1:200, Abcam, abab59400), mouse-anti-KRT6 (Abcam, ab18586), recombinant rabbit anti-PAX8 (Abcam, ab239363), goat anti- Chlamydia trachomatis Major Outer Membrane Protein (MOMP) (1:500, BIO-RAD, 1990-0804) and for labeling the DNA, DAPI (Roche, 10236276001) was used.

Techniques: Derivative Assay, Staining, Gene Expression, Activity Assay

Journal: bioRxiv

Article Title: Epidermal loss of PRMT5 leads to the emergence of an atypical basal keratinocyte-like cell population and defective skin stratification

doi: 10.1101/2024.11.08.620904

Figure Lengend Snippet: ( A-H ) Coronal sections from E15.5 CTRL (A, C, E, G) or Prmt5 cKO (B, D, F, H) embryonic heads, stained for α-KRT6 (A-B), α-COL4 (C-D), α-CLDN1 (E-F), or α-VCL (G-H) by immunofluorescence. Note, sections shown are the craniofacial epidermis, lateral to the oral cavity, in a coronal plane. White dashed lines demarcate the boundary of the dermis and epidermis. All sectional immunofluorescence images are five Z-stacks compiled with ‘Max Intensity’ in FIJI. Scale bar = 10µm.

Article Snippet: Primary antibodies include: α-CDKN1A (1:50, ProteinTech, 10355-1-AP), α-CLDN1 (1:60, Abcam, ab15098), α-COL4 (1:500, Invitrogen, PA1-85320), α-eGFP (1:500, Novus Biologicals, NB600-308), α-FLG (1:500, Biolegend, 905804), α-IGFBP2 (1:200, Bioss, bs-1108R), α-KI67 (1:200, Abcam, ab16667), α-KRT6 (1:500, Biolegend, PRB-169F), α-KRT10 (1:500, Biolegend, 905404), α-KRT14 (1:20, SantaCruz, sc-53253), α-KRT17 (1:1000, Proteintech, 17516-1-AP), α-NF (0.5ug/ml, Hybridoma, AB531793), α-PRMT5 (1:200, Millipore, 07-405), α-TRP63 (1:500, Cell Signaling, 13109S), and α-VCL (1:300, Sigma, V9131).

Techniques: Staining, Immunofluorescence