anti ki67 antibody  (Boster Bio)

Journal: Gut

Article Title: A purified membrane protein from Akkermansia muciniphila or the pasteurised bacterium blunts colitis associated tumourigenesis by modulation of CD8+ T cells in mice

doi: 10.1136/gutjnl-2019-320105

Figure Lengend Snippet: Supplementation with pasteurised A. muciniphila or Amuc_1100 blunted tumourigenesis induced by azoxymethane (AOM)/DSS. (A) Weight changes are expressed as the mean change from the starting weight. (B) DAI, (C) spleen weight, (D) tumour development, number (E) and tumour area (F) were analysed. (G) Representative histological images of colon tissues by H E staining. Scale bars, 200 µm. IHC staining and quantitation of γH2AX (8 weeks, H), cleaved-caspase 3 (23 weeks, I) and Ki67 (23 weeks, J) in the proximal colon. Scale bars, 50 µm. Data are presented as the mean±SEM and were analysed by ordinary one-way ANOVA with Tukey’s multiple comparisons. *P

Article Snippet: The sections were then blocked and stained with CD8a, F4/80, cleaved-caspase 3, γH2AX or Ki67 antibodies, followed by corresponding secondary antibody and a Streptavidin Biotin Complex kit (Boster BioEngineering, Wuhan, China).

Techniques: Staining, Immunohistochemistry, Quantitation Assay

Journal: Frontiers in Medicine

Article Title: MK2 Is Required for Neutrophil-Derived ROS Production and Inflammatory Bowel Disease

doi: 10.3389/fmed.2020.00207

Figure Lengend Snippet: Histological changes of AB-PAS + goblet cells and proliferative cells (Ki67 + ) in MK2 lyz2−WT and MK2 lyz2−KO mice after 7 days' treatment of 3.5% DSS or water control. (A) Representative images showing AB-PAS + goblet cells in colon sections from MK2 lyz2−WT and MK2 lyz2−KO mice. Scale bars: 100 μm (upper), 40 μm (lower). (B) Statistical analysis of AB-PAS + goblet cells in each crypt. (C) Representative images showing proliferative cells (Ki67 + ) in colon of DSS treatment mice. Scale bars, 100 μm (upper), 40 μm (lower). (D) Statistical analysis of Ki67 + cells in each crypt. The results were shown as means ± SEM. * P

Article Snippet: Then, sections were incubated with anti-Ki67 antibody overnight at 4°C, followed by incubation with horseradish peroxidase (HRP) secondary antibodies for 20 min.

Techniques: Mouse Assay

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: IL-33 facilitates proliferation of colorectal cancer dependent on COX2/PGE2

doi: 10.1186/s13046-018-0839-7

Figure Lengend Snippet: COX2/PGE 2 mediates the proliferation promoting function of IL-33. a , b The relative mRNA levels of Ki67 ( a ) and PCNA ( b ) in primary CRC cells responding to rhIL-33 (100 ng/mL) incubation and/ or indicated inhibitors (SB203580, 10 μg/mL; PD98059, 20 μg/mL; SP600125, 10 μg/mL; BIX01294, 2 μM; 5Aza, 10 μM; SC560, 0.1 μg/mL; celecoxib, 20 μg/mL) for 24 h. c The relative mRNA levels of Ki67 and PCNA in HT-29 cells incubated with rhIL-33 (100 ng/mL) or/ and celecoxib (CXB) (20 μg/mL) in medium for 24 h. d The relative mRNA levels of Ki67 and PCNA in MC38 cells incubated with rmIL-33 (100 ng/mL) or/ and celecoxib (CXB) (20 μg/mL) in medium for 24 h. e , f The mRNA ( e ) and protein ( f ) expression of COX2 in primary CRC cells incubated with 0, 50 or 100 ng/mL of rhIL-33 in medium for 24 h. g PGE 2 concentrations in the supernatants of primary CRC cells incubated with rhIL-33-contained RPMI medium or blank RPMI medium for 48 h. h Cell viabilities of primary CRC cells incubated with or without PGE 2 (50 ng/mL) in medium. i The flat colony formation of primary CRC cells incubated for 15 days in medium containing different factors as indicated (IL-33, 100 ng/mL; celecoxib, 20 μg/mL; anti-PGE 2 , 2 μg/mL). The representative images of colonies and the statistical data are shown. Three parallel wells were set for each treatment. Each experiment was performed three times. Data expressed as mean ± SEM. * P

Article Snippet: The sections were labeled with anti-Ki67 antibody (Arigo, 1:200) and anti-PCNA antibody (Boster, 1:200).

Techniques: Incubation, Expressing

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: IL-33 facilitates proliferation of colorectal cancer dependent on COX2/PGE2

doi: 10.1186/s13046-018-0839-7

Figure Lengend Snippet: IL-33 promotes CRC proliferation both in vivo and in vitro. a Correlation between IL-33 transcripts and the genes involved in the regulation of cell proliferation in CRC. Gene set enrichment analysis was performed using CRC TCGA database. NES = 1.03, P = 0.03. b Growth curves of MC38 tumors inoculated in IL-33 transgenic mice (IL-33 TG) or wild-type mice (WT). n = 7. c , d Immunohistochemical staining of Ki67 ( c ) and PCNA ( d ) in the MC38 tumors recovered from wild-type and IL-33 transgenic mice at Day 22 post inoculation. The representative images and the statistical proportions of positive cells are shown. Scale bar, 50 μm. n = 7. Data expressed as mean ± SEM. **, P

Article Snippet: The sections were labeled with anti-Ki67 antibody (Arigo, 1:200) and anti-PCNA antibody (Boster, 1:200).

Techniques: In Vivo, In Vitro, Transgenic Assay, Mouse Assay, Immunohistochemistry, Staining

Journal: Cell Death & Disease

Article Title: Tumor-suppressive function of long noncoding RNA MALAT1 in glioma cells by downregulation of MMP2 and inactivation of ERK/MAPK signaling

doi: 10.1038/cddis.2015.407

Figure Lengend Snippet: Effects of upregulation and downregulation of MALAT1 on U87 and U251 cell proliferation. ( a and b ) Cellular proliferation of untransfected or transfected U87 and U251 cells was measured using a CCK-8 assay daily for 3 days. ( c ) Cellular proliferation of untransfected or transfected U87 and U251 cells was measured by testing the expression of Ki-67. ( d and e ) The percentage of Ki-67-positive cells was calculated. Results are expressed as mean±S.D. from three independent experiments ( P

Article Snippet: Slides were incubated with primary antibodies against Ki-67 (Boster Bioengineering Co., Wuhan, China), and with antibodies against MMP2 and TIMP3 (Abcam).

Techniques: Transfection, CCK-8 Assay, Expressing