Journal: Nature Communications

Article Title: Efficient intervention for pulmonary fibrosis via mitochondrial transfer promoted by mitochondrial biogenesis

doi: 10.1038/s41467-023-41529-7

Figure Lengend Snippet: a Schematic illustration and representative image of EpCAM immunostaining in primary human lung epithelial cells (hLECs). Scale bar, 50 μm. b Mitochondrial transfer rates from the indicated hMSC to the primary hLEC ( n = 3 biologically independent cells). c Relative intracellular ROS levels ( n = 3 biologically independent cells), d TGF- β expression levels ( n = 4 biologically independent cells), and e Viability of BLM-treated hLEC after the indicated treatment using different engineered hMSCs ( n = 3 biologically independent cells). f Schematic illustration showing the preparation of the 3D multicellular human fibrotic model. g Representative immunostaining images showing the expression of α -smooth muscle actin ( α -SMA), vimentin, collagen-I, and Ki-67 in the healthy and fibrotic multicellular human spheroid models. Blue fluorescent signals indicate the cell nuclei and green signals indicate the biomarkers. Scale bar, 20 μm. h Mitochondrial transfer rates of different engineered hMSC in fibrotic human lung spheroids ( n = 3 biologically independent experiments). i Relative intracellular ROS levels ( n = 3 biologically independent experiments) and j TGF- β expression levels of fibrotic human lung spheroids after the indicated treatment using different engineered hMSCs ( n = 4 biologically independent experiments). k Viability of fibrotic human lung spheroids after the indicated treatment using different engineered hMSCs ( n = 3 biologically independent experiments). Data are presented as means ± SD. Statistical significance was analyzed using ordinary one-way ANOVA. ECs epithelial cells.

Article Snippet: In brief, the collected samples (three biologically independent samples for each group) were firstly labeled with the primary antibodies of α -SMA (Boster, BM3902, 1:100), collagen-I (Proteintech, 14695-1-AP, 1:500), vimentin (Boster, BM4029, 1:100) and Ki-67 (Boster, PB9026, 1:200), followed by the incubation with the secondary antibody of FITC labeled goat anti-rabbit IgG (H + L) (Boster, BA1105, 1:100) for 1 h. The fluorescence signals were observed by CLSM and the density of fluorescence signals was quantified using Image J software.

Techniques: Immunostaining, Expressing

Journal: PeerJ

Article Title: Dieting alleviates hyperuricemia and organ injuries in uricase-deficient rats via down-regulating cell cycle pathway

doi: 10.7717/peerj.15999

Figure Lengend Snippet: (A) Casp3, Cdk1 and Ki67 in the liver was evaluated at mRNA level, and a high value of FPKM means a high expression; (B) Casp3, Cdk1 and Ki67 in the liver was evaluated by Western blot; C, Results of B were quantified by relative brightness. FPKM, Fragments Per Kilobase of exon model per Million mapped fragments. Control, control group, KDY rats were given free approach to food and water; 75% dieting, 75% dieting group, rats were given 75% food of control group at the same age in days and given free access to water. * P < 0.05 vs control group, Student’s t -test, two-tailed.

Article Snippet: Hematoxylin-eosin (HE) staining kits, mouse anti- β -actin antibody (BM0627), rabbit anti-CASP3 antibody (BA2142), rabbit anti-CDK1 antibody (PB9533), rabbit anit-Ki67 antibody (M00254-4), and horseradish peroxidase (HRP) conjugated goat anti-rabbit/mouse IgG (H+L) (BA1056) were all purchased from Boster Biological Engineering Co., Ltd (Wuhan, China).

Techniques: Expressing, Western Blot, Two Tailed Test

Journal: Journal of Oral Biology and Craniofacial Research

Article Title: P63 and Ki-67 expression in radicular cyst

doi: 10.1016/j.jobcr.2023.06.008

Figure Lengend Snippet: Expression of P63 and Ki-67, and degree of inflammatory infiltrate in the studied radicular cysts.

Article Snippet: Both primary antibodies were diluted at 1:100 and consisted of polyclonal rabbit anti-human Ki-67 antigen (Catalog No. PB9026, Wuhan Boster Biological Technology, Ltd., Wuhan, China) and polyclonal rabbit anti-human P63 antigen which mainly recognizes ΔNp63 isoforms (Catalog No. BA1326, Wuhan Boster Biological Technology, Ltd., Wuhan, China).

Techniques: Expressing

Journal: Journal of Oral Biology and Craniofacial Research

Article Title: P63 and Ki-67 expression in radicular cyst

doi: 10.1016/j.jobcr.2023.06.008

Figure Lengend Snippet: Immunohistochemical staining of P63 and Ki-67 in radicular cysts (magnification, ×400). (A) Strong P63 positivity through the epithelium and connective tissue, (B) moderate P63 positivity mainly through the epithelium, (C) strong P63 positivity in the inflammatory cells throughout the connective tissue, (D) absence of P63 staining in the epithelium and connective tissue wall, (E) moderate Ki-67 staining in the nucleus of the positive cells throughout the whole epithelium, and (F) strong Ki-67 positivity throughout epithelium and in many cells in the connective tissue capsule.

Article Snippet: Both primary antibodies were diluted at 1:100 and consisted of polyclonal rabbit anti-human Ki-67 antigen (Catalog No. PB9026, Wuhan Boster Biological Technology, Ltd., Wuhan, China) and polyclonal rabbit anti-human P63 antigen which mainly recognizes ΔNp63 isoforms (Catalog No. BA1326, Wuhan Boster Biological Technology, Ltd., Wuhan, China).

Techniques: Immunohistochemical staining, Staining

Journal: Journal of Oral Biology and Craniofacial Research

Article Title: P63 and Ki-67 expression in radicular cyst

doi: 10.1016/j.jobcr.2023.06.008

Figure Lengend Snippet: Correlations between P63, Ki-67, inflammatory infiltrate, and size of the studied radicular cysts.

Article Snippet: Both primary antibodies were diluted at 1:100 and consisted of polyclonal rabbit anti-human Ki-67 antigen (Catalog No. PB9026, Wuhan Boster Biological Technology, Ltd., Wuhan, China) and polyclonal rabbit anti-human P63 antigen which mainly recognizes ΔNp63 isoforms (Catalog No. BA1326, Wuhan Boster Biological Technology, Ltd., Wuhan, China).

Techniques: Expressing

Journal: iScience

Article Title: CD168 + macrophages promote hepatocellular carcinoma tumor stemness and progression through TOP2A /β-catenin/ YAP1 axis

doi: 10.1016/j.isci.2023.106862

Figure Lengend Snippet: TOP2A knockdown inhibited the proliferation of HCC cell lines (A) Western blot of TOP2A expression after transfection with MHCC97L and Hep3B2.1-7 (si Ctrl , si TOP2A #1 or si TOP2A #2). (B) Downregulation of TOP2A reduced the mean colony number in the colony formation assay. (C) CCK-8 assays revealed that downregulation of TOP2A decreased the growth rate of indicated cells. (D) Western blot showed the expression of Ki67 and PCNA in MHCC97L and Hep3B2.1-7 cells after the knockdown of TOP2A. (E) Flow cytometry to detect the cell cycle of HCC cells (MHCC97L and Hep3B2.1-7) transfected with si-Ctrl or si-TOP2A. (F) Tumor growth and weight of tumors in xenograft mice injected with shTOP2A or control cells at the indicated times. (G) The expression of Ki67 in tumors was determined by immunohistochemistry. Data are represented as mean ± SEM; ∗∗∗p< 0.001, ∗∗p< 0.01, ∗p< 0.05.

Article Snippet: Next, the proteins were incubated with primary antibodies and the primary antibodies used for analysis were as follows: rabbit anti-TOP2A (1:2,500; Cat#12286, CST), rabbit anti-CENPF (1:200, Cat#ab224813, Abcam), rabbit anti-β-actin (1:10,000; Cat#BM0627, Boster), rabbit anti-Ki-67 (1:2,000; Boster, M00254-9), rabbit anti-PCNA (1:2,000;Boster, M00125-3), rabbit anti-Cyclin A1 (1:1,000;abcam ab53699), rabbit anti-Cyclin B1 (1:2,000;abcam, ab181593), rabbit anti-Cyclin B2 (1:1,000; abcam, ab185622), rabbit anti-Cyclin D1 (1:200; abcam, ab16663), rabbit anti-Cyclin E1 (1:1,000;abcam, abab33911), rabbit anti-YAP1 (1:5,000;abcam, ab52771), rabbit anti-Phospho-YAP1 (1:1,000;CST, 13008), rabbit anti-TAZ (1:500;abcam ab224239), rabbit anti-TEAD4 (1:1,000;abcam ab58310), rabbit anti-GAPDH (1:2,000;Boster A00227-1), and rabbit anti-histone-H3 (1:1,000;Boster A12477-2).

Techniques: Western Blot, Expressing, Transfection, Colony Assay, CCK-8 Assay, Flow Cytometry, Injection, Immunohistochemistry

Journal: iScience

Article Title: CD168 + macrophages promote hepatocellular carcinoma tumor stemness and progression through TOP2A /β-catenin/ YAP1 axis

doi: 10.1016/j.isci.2023.106862

Figure Lengend Snippet:

Article Snippet: Next, the proteins were incubated with primary antibodies and the primary antibodies used for analysis were as follows: rabbit anti-TOP2A (1:2,500; Cat#12286, CST), rabbit anti-CENPF (1:200, Cat#ab224813, Abcam), rabbit anti-β-actin (1:10,000; Cat#BM0627, Boster), rabbit anti-Ki-67 (1:2,000; Boster, M00254-9), rabbit anti-PCNA (1:2,000;Boster, M00125-3), rabbit anti-Cyclin A1 (1:1,000;abcam ab53699), rabbit anti-Cyclin B1 (1:2,000;abcam, ab181593), rabbit anti-Cyclin B2 (1:1,000; abcam, ab185622), rabbit anti-Cyclin D1 (1:200; abcam, ab16663), rabbit anti-Cyclin E1 (1:1,000;abcam, abab33911), rabbit anti-YAP1 (1:5,000;abcam, ab52771), rabbit anti-Phospho-YAP1 (1:1,000;CST, 13008), rabbit anti-TAZ (1:500;abcam ab224239), rabbit anti-TEAD4 (1:1,000;abcam ab58310), rabbit anti-GAPDH (1:2,000;Boster A00227-1), and rabbit anti-histone-H3 (1:1,000;Boster A12477-2).

Techniques: Recombinant, Preserving, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, SYBR Green Assay, Software

Journal: iScience

Article Title: CD168 + macrophages promote hepatocellular carcinoma tumor stemness and progression through TOP2A /β-catenin/ YAP1 axis

doi: 10.1016/j.isci.2023.106862

Figure Lengend Snippet: TOP2A knockdown inhibited the proliferation of HCC cell lines (A) Western blot of TOP2A expression after transfection with MHCC97L and Hep3B2.1-7 (si Ctrl , si TOP2A #1 or si TOP2A #2). (B) Downregulation of TOP2A reduced the mean colony number in the colony formation assay. (C) CCK-8 assays revealed that downregulation of TOP2A decreased the growth rate of indicated cells. (D) Western blot showed the expression of Ki67 and PCNA in MHCC97L and Hep3B2.1-7 cells after the knockdown of TOP2A. (E) Flow cytometry to detect the cell cycle of HCC cells (MHCC97L and Hep3B2.1-7) transfected with si-Ctrl or si-TOP2A. (F) Tumor growth and weight of tumors in xenograft mice injected with shTOP2A or control cells at the indicated times. (G) The expression of Ki67 in tumors was determined by immunohistochemistry. Data are represented as mean ± SEM; ∗∗∗p< 0.001, ∗∗p< 0.01, ∗p< 0.05.

Article Snippet: Rabbit monoclonal anti-Ki-67 , Boster , Cat#M00254-9.

Techniques: Western Blot, Expressing, Transfection, Colony Assay, CCK-8 Assay, Flow Cytometry, Injection, Immunohistochemistry

Journal: iScience

Article Title: CD168 + macrophages promote hepatocellular carcinoma tumor stemness and progression through TOP2A /β-catenin/ YAP1 axis

doi: 10.1016/j.isci.2023.106862

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti-Ki-67 , Boster , Cat#M00254-9.

Techniques: Recombinant, Preserving, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, SYBR Green Assay, Software

Journal: iScience

Article Title: CD168 + macrophages promote hepatocellular carcinoma tumor stemness and progression through TOP2A /β-catenin/ YAP1 axis

doi: 10.1016/j.isci.2023.106862

Figure Lengend Snippet: TOP2A knockdown inhibited the proliferation of HCC cell lines (A) Western blot of TOP2A expression after transfection with MHCC97L and Hep3B2.1-7 (si Ctrl , si TOP2A #1 or si TOP2A #2). (B) Downregulation of TOP2A reduced the mean colony number in the colony formation assay. (C) CCK-8 assays revealed that downregulation of TOP2A decreased the growth rate of indicated cells. (D) Western blot showed the expression of Ki67 and PCNA in MHCC97L and Hep3B2.1-7 cells after the knockdown of TOP2A. (E) Flow cytometry to detect the cell cycle of HCC cells (MHCC97L and Hep3B2.1-7) transfected with si-Ctrl or si-TOP2A. (F) Tumor growth and weight of tumors in xenograft mice injected with shTOP2A or control cells at the indicated times. (G) The expression of Ki67 in tumors was determined by immunohistochemistry. Data are represented as mean ± SEM; ∗∗∗p< 0.001, ∗∗p< 0.01, ∗p< 0.05.

Article Snippet: The sections were blocked with 10% goat serum and incubated with anti- TOP2A (1:200, Cat#12286, CST) or anti- CENPF (1:200, Cat#ab224813, Abcam), or anti-ki-67 (1:200, M00254-9, Boster) antibodies at 4°Cat night.

Techniques: Western Blot, Expressing, Transfection, Colony Assay, CCK-8 Assay, Flow Cytometry, Injection, Immunohistochemistry

Journal: iScience

Article Title: CD168 + macrophages promote hepatocellular carcinoma tumor stemness and progression through TOP2A /β-catenin/ YAP1 axis

doi: 10.1016/j.isci.2023.106862

Figure Lengend Snippet:

Article Snippet: The sections were blocked with 10% goat serum and incubated with anti- TOP2A (1:200, Cat#12286, CST) or anti- CENPF (1:200, Cat#ab224813, Abcam), or anti-ki-67 (1:200, M00254-9, Boster) antibodies at 4°Cat night.

Techniques: Recombinant, Preserving, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, SYBR Green Assay, Software

Journal: Biology Direct

Article Title: Upregulation of long intergenic non-coding RNA LINC00326 inhibits non-small cell lung carcinoma progression by blocking Wnt/β-catenin pathway through modulating the miR-657/dickkopf WNT signaling pathway inhibitor 2 axis

doi: 10.1186/s13062-023-00359-9

Figure Lengend Snippet: LINC00326 downregulation accelerates tumor growth in vivo. a Appearance of tumors four weeks after injection, showing the blank control, negative control, LINC00326 overexpression, shCTRL, and LINC00326 knockdown. b Tumor volumes in the five groups (mean ± SD) at time of sacrifice. c , d LINC00326 and miR-657 mRNA expression in tumors from blank, OE-CTRL, OE- LINC00326, shCTRL, and shLINC00326 groups. e Wnt1, Wnt3A, and β-catenin protein levels in tumors from blank, OE-CTRL, OE- LINC00326, shCTRL, and shLINC00326 groups. f–h Ki67 and TUNEL staining in blank, OE-CTRL, OE- LINC00326, shCTRL, and shLINC00326 groups, respectively (× 200, scale bars, 100 µm); n = 3. Data are presented as mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001

Article Snippet: Anti-Ki-67 antibody (BOSTER, A00254) was used for IHC, as per a previously reported method [ ].

Techniques: In Vivo, Injection, Negative Control, Over Expression, Expressing, TUNEL Assay, Staining