anti ki 67 antibody  (Boster Bio)


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    Structured Review

    Boster Bio anti ki 67 antibody
    SOAT1 is a downstream target of miR-30a-3p. A RKO and SW480 cells were transfected with miR-30a-3p mimics or inhibitors. SOAT1 expression was scouted by qRT-PCR. B Relative activities of luciferase were measured in HEK293T cells after transfection with SOAT1-Mut or SOAT1-WT and miR-30a-3p mimics or mimic NC. C Overexpression of miR-30a-3p restrained the protein production of SOAT1. Suppression of miR-30a-3p raised the expression of SOAT1 protein. D Knockdown of circLDLR (sh-circLDLR) inhibited the protein expression of SOAT1. Overexpression of circLDLR (circLDLR-OE) increased the protein expression of SOAT1. E Knockdown of miR-30a-3p reversed the sh-circLDLR-induced downregulation of SOAT1 expression in RKO cells. Overexpression of miR-30a-3p reversed the circLDLR-OE-induced upregulation of SOAT1 expression in SW480 cells. GAPDH deeded as a loading control. F EdU analysis of the <t>cell</t> <t>proliferation</t> ability in SW480 and HT29 transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors. G Cell migration and invasion in SW480 and HT29 transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors were examined. H The contents of T-CHO and LDL-C in SW480 and HT29 cells transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors were assessed. I EdU analysis of the cell proliferation ability in SW480 and HT29 transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE. J Cell behaviors of migration and invasion in HT29 and SW480 cells transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE were examined. K The contents of T-CHO and LDL-C in SW480 and HT29 cells transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE were assessed. Data are presented as the mean ± SD of three independent experiments. * P
    Anti Ki 67 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ki 67 antibody/product/Boster Bio
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    anti ki 67 antibody - by Bioz Stars, 2022-09
    94/100 stars

    Images

    1) Product Images from "Circular RNA circLDLR facilitates cancer progression by altering the miR-30a-3p/SOAT1 axis in colorectal cancer"

    Article Title: Circular RNA circLDLR facilitates cancer progression by altering the miR-30a-3p/SOAT1 axis in colorectal cancer

    Journal: Cell Death Discovery

    doi: 10.1038/s41420-022-01110-5

    SOAT1 is a downstream target of miR-30a-3p. A RKO and SW480 cells were transfected with miR-30a-3p mimics or inhibitors. SOAT1 expression was scouted by qRT-PCR. B Relative activities of luciferase were measured in HEK293T cells after transfection with SOAT1-Mut or SOAT1-WT and miR-30a-3p mimics or mimic NC. C Overexpression of miR-30a-3p restrained the protein production of SOAT1. Suppression of miR-30a-3p raised the expression of SOAT1 protein. D Knockdown of circLDLR (sh-circLDLR) inhibited the protein expression of SOAT1. Overexpression of circLDLR (circLDLR-OE) increased the protein expression of SOAT1. E Knockdown of miR-30a-3p reversed the sh-circLDLR-induced downregulation of SOAT1 expression in RKO cells. Overexpression of miR-30a-3p reversed the circLDLR-OE-induced upregulation of SOAT1 expression in SW480 cells. GAPDH deeded as a loading control. F EdU analysis of the cell proliferation ability in SW480 and HT29 transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors. G Cell migration and invasion in SW480 and HT29 transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors were examined. H The contents of T-CHO and LDL-C in SW480 and HT29 cells transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors were assessed. I EdU analysis of the cell proliferation ability in SW480 and HT29 transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE. J Cell behaviors of migration and invasion in HT29 and SW480 cells transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE were examined. K The contents of T-CHO and LDL-C in SW480 and HT29 cells transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE were assessed. Data are presented as the mean ± SD of three independent experiments. * P
    Figure Legend Snippet: SOAT1 is a downstream target of miR-30a-3p. A RKO and SW480 cells were transfected with miR-30a-3p mimics or inhibitors. SOAT1 expression was scouted by qRT-PCR. B Relative activities of luciferase were measured in HEK293T cells after transfection with SOAT1-Mut or SOAT1-WT and miR-30a-3p mimics or mimic NC. C Overexpression of miR-30a-3p restrained the protein production of SOAT1. Suppression of miR-30a-3p raised the expression of SOAT1 protein. D Knockdown of circLDLR (sh-circLDLR) inhibited the protein expression of SOAT1. Overexpression of circLDLR (circLDLR-OE) increased the protein expression of SOAT1. E Knockdown of miR-30a-3p reversed the sh-circLDLR-induced downregulation of SOAT1 expression in RKO cells. Overexpression of miR-30a-3p reversed the circLDLR-OE-induced upregulation of SOAT1 expression in SW480 cells. GAPDH deeded as a loading control. F EdU analysis of the cell proliferation ability in SW480 and HT29 transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors. G Cell migration and invasion in SW480 and HT29 transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors were examined. H The contents of T-CHO and LDL-C in SW480 and HT29 cells transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors were assessed. I EdU analysis of the cell proliferation ability in SW480 and HT29 transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE. J Cell behaviors of migration and invasion in HT29 and SW480 cells transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE were examined. K The contents of T-CHO and LDL-C in SW480 and HT29 cells transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE were assessed. Data are presented as the mean ± SD of three independent experiments. * P

    Techniques Used: Transfection, Expressing, Quantitative RT-PCR, Luciferase, Over Expression, Migration

    CircLDLR overexpression facilitates CRC malignant behaviour and increases cholesterol levels in vitro. A The expression of circLDLR in RKO and HCT116 cells transfected with a circLDLR-overexpression vector (circLDLR-OE) or the control vector (Vector) was assessed by qRT-PCR. B Cell proliferation was scouted at the indicated time points by CCK-8 assays evaluating RKO cells and HCT116 cells transfected with circLDLR-OE or Vector. C EdU analysis of the proliferative ability of RKO cells and HCT116 cells transfected with circLDLR-OE or Vector. Representative images are shown. Scale bar, 200 μm. Statistical analysis of the EdU-positive cell percentage in transfected CRC cells is shown in the bar graph. D The cell migration and invasion of CRC cells were examined by Transwell assays after cells were transfected with circLDLR-OE or Vector. Representative images are shown. Scale bar, 200 μm. Statistical analysis of the migrated and invaded cell numbers is shown in the bar graph. E e The production of T-CHO and LDL-C in CRC cells transfected with circLDLR-OE or Vector were appraised. Data are presented as the mean ± SD of three independent experiments. * P
    Figure Legend Snippet: CircLDLR overexpression facilitates CRC malignant behaviour and increases cholesterol levels in vitro. A The expression of circLDLR in RKO and HCT116 cells transfected with a circLDLR-overexpression vector (circLDLR-OE) or the control vector (Vector) was assessed by qRT-PCR. B Cell proliferation was scouted at the indicated time points by CCK-8 assays evaluating RKO cells and HCT116 cells transfected with circLDLR-OE or Vector. C EdU analysis of the proliferative ability of RKO cells and HCT116 cells transfected with circLDLR-OE or Vector. Representative images are shown. Scale bar, 200 μm. Statistical analysis of the EdU-positive cell percentage in transfected CRC cells is shown in the bar graph. D The cell migration and invasion of CRC cells were examined by Transwell assays after cells were transfected with circLDLR-OE or Vector. Representative images are shown. Scale bar, 200 μm. Statistical analysis of the migrated and invaded cell numbers is shown in the bar graph. E e The production of T-CHO and LDL-C in CRC cells transfected with circLDLR-OE or Vector were appraised. Data are presented as the mean ± SD of three independent experiments. * P

    Techniques Used: Over Expression, In Vitro, Expressing, Transfection, Plasmid Preparation, Quantitative RT-PCR, CCK-8 Assay, Migration

    CircLDLR suppression inhibits CRC malignant behaviour and increases cholesterol levels in vitro. A The circLDLR and mLDLR expression was assessed in RKO and HCT116 cells transfected with five independent siRNAs targeting circLDLR by qRT-PCR. B Cell proliferation was scouted at the indicated time points by CCK-8 assays evaluating HCT116 cells and RKO cells stimulated with si-NC or si-circLDLR. C EdU analysis of the proliferative ability of HCT116 cells and RKO cells treated with si-NC or si-circLDLR. Representative images are shown. Scale bar, 200 μm. Statistical analysis of the EdU-positive cell percentage in transfected GC cells is shown in the bar graph. D The cell migration and invasion of CRC cells were examined by Transwell assays after cells were transfected with si-circLDLR or si-NC. Representative images are shown. Scale bar, 200 μm. Statistical analysis of the migrated and invaded cell numbers is shown in the bar graph. E The production of T-CHO and LDL-C was assessed in CRC cells transfected with si-NC or si-circLDLR. Data are presented as the mean ± SD of three independent experiments. * P
    Figure Legend Snippet: CircLDLR suppression inhibits CRC malignant behaviour and increases cholesterol levels in vitro. A The circLDLR and mLDLR expression was assessed in RKO and HCT116 cells transfected with five independent siRNAs targeting circLDLR by qRT-PCR. B Cell proliferation was scouted at the indicated time points by CCK-8 assays evaluating HCT116 cells and RKO cells stimulated with si-NC or si-circLDLR. C EdU analysis of the proliferative ability of HCT116 cells and RKO cells treated with si-NC or si-circLDLR. Representative images are shown. Scale bar, 200 μm. Statistical analysis of the EdU-positive cell percentage in transfected GC cells is shown in the bar graph. D The cell migration and invasion of CRC cells were examined by Transwell assays after cells were transfected with si-circLDLR or si-NC. Representative images are shown. Scale bar, 200 μm. Statistical analysis of the migrated and invaded cell numbers is shown in the bar graph. E The production of T-CHO and LDL-C was assessed in CRC cells transfected with si-NC or si-circLDLR. Data are presented as the mean ± SD of three independent experiments. * P

    Techniques Used: In Vitro, Expressing, Transfection, Quantitative RT-PCR, CCK-8 Assay, Migration

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    Boster Bio ki 67
    miR-16 reduced <t>Ki-67</t> (a), MMP9 (b), and nuclear factor-κB1 (NF-κB1) (c) expression in an in vivo mouse model using U87 glioma cells. miR-16, microRNA-16.
    Ki 67, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ki 67/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ki 67 - by Bioz Stars, 2022-09
    94/100 stars
      Buy from Supplier

    94
    Boster Bio ki67 antibodies
    Supplementation with pasteurised A. muciniphila or Amuc_1100 blunted tumourigenesis induced by azoxymethane (AOM)/DSS. (A) Weight changes are expressed as the mean change from the starting weight. (B) DAI, (C) spleen weight, (D) tumour development, number (E) and tumour area (F) were analysed. (G) Representative histological images of colon tissues by H E staining. Scale bars, 200 µm. IHC staining and quantitation of γH2AX (8 weeks, H), cleaved-caspase 3 (23 weeks, I) and <t>Ki67</t> (23 weeks, J) in the proximal colon. Scale bars, 50 µm. Data are presented as the mean±SEM and were analysed by ordinary one-way ANOVA with Tukey’s multiple comparisons. *P
    Ki67 Antibodies, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ki67 antibodies/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ki67 antibodies - by Bioz Stars, 2022-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    miR-16 reduced Ki-67 (a), MMP9 (b), and nuclear factor-κB1 (NF-κB1) (c) expression in an in vivo mouse model using U87 glioma cells. miR-16, microRNA-16.

    Journal: Cancer Science

    Article Title: MicroRNA-16 inhibits glioma cell growth and invasion through suppression of BCL2 and the nuclear factor-κB1/MMP9 signaling pathway

    doi: 10.1111/cas.12351

    Figure Lengend Snippet: miR-16 reduced Ki-67 (a), MMP9 (b), and nuclear factor-κB1 (NF-κB1) (c) expression in an in vivo mouse model using U87 glioma cells. miR-16, microRNA-16.

    Article Snippet: Slides were incubated with primary antibodies against MMP9, Ki-67 (Boster Bioengineering, Wuhan, China) and NF-κB1.

    Techniques: Expressing, In Vivo

    Effects of TGF- α and its neutralizing antibodies on the EMT of BEAS-2B cells and tumor growth. (a) The effects of TGF- α (0.4 ng/mL) and anti-TGF- α (1.6 ng/mL) on the proliferation of BEAS-2B cells, control group treated only by BPDE. (b) The colony formation rate of BEAS-2B cells. (c) The growth rate curve of xenografted tumors. (d, e) Ki67, PCNA, and proteins involved EMT in tumor tissue were detected by western blotting. (f) The apoptosis of the tumor tissue was detected using the TUNEL assay, and the markers of EMT in tumor tissue were detected by immunohistochemistry in tumor tissues.

    Journal: BioMed Research International

    Article Title: Blockage of TGF-α Induced by Spherical Silica Nanoparticles Inhibits Epithelial-Mesenchymal Transition and Proliferation of Human Lung Epithelial Cells

    doi: 10.1155/2019/8231267

    Figure Lengend Snippet: Effects of TGF- α and its neutralizing antibodies on the EMT of BEAS-2B cells and tumor growth. (a) The effects of TGF- α (0.4 ng/mL) and anti-TGF- α (1.6 ng/mL) on the proliferation of BEAS-2B cells, control group treated only by BPDE. (b) The colony formation rate of BEAS-2B cells. (c) The growth rate curve of xenografted tumors. (d, e) Ki67, PCNA, and proteins involved EMT in tumor tissue were detected by western blotting. (f) The apoptosis of the tumor tissue was detected using the TUNEL assay, and the markers of EMT in tumor tissue were detected by immunohistochemistry in tumor tissues.

    Article Snippet: The separated protein bands were transferred to a polyvinylidene difluoride (PVDF) membrane after blocking with defatted milk at 37°C for 2 hours and incubated at 4°C overnight with anti-PCNA antibody (2586, Cell Signaling Technology, USA) and ki67 antibody (BM438, Boster, China); antibodies for proteins involved in EMT used the same as IHC method, goat anti-mouse and rabbit (ab205719, ab205718) as second antibody.

    Techniques: Western Blot, TUNEL Assay, Immunohistochemistry

    Effects of upregulation and downregulation of MALAT1 on U87 and U251 cell proliferation. ( a and b ) Cellular proliferation of untransfected or transfected U87 and U251 cells was measured using a CCK-8 assay daily for 3 days. ( c ) Cellular proliferation of untransfected or transfected U87 and U251 cells was measured by testing the expression of Ki-67. ( d and e ) The percentage of Ki-67-positive cells was calculated. Results are expressed as mean±S.D. from three independent experiments ( P

    Journal: Cell Death & Disease

    Article Title: Tumor-suppressive function of long noncoding RNA MALAT1 in glioma cells by downregulation of MMP2 and inactivation of ERK/MAPK signaling

    doi: 10.1038/cddis.2015.407

    Figure Lengend Snippet: Effects of upregulation and downregulation of MALAT1 on U87 and U251 cell proliferation. ( a and b ) Cellular proliferation of untransfected or transfected U87 and U251 cells was measured using a CCK-8 assay daily for 3 days. ( c ) Cellular proliferation of untransfected or transfected U87 and U251 cells was measured by testing the expression of Ki-67. ( d and e ) The percentage of Ki-67-positive cells was calculated. Results are expressed as mean±S.D. from three independent experiments ( P

    Article Snippet: Slides were incubated with primary antibodies against Ki-67 (Boster Bioengineering Co., Wuhan, China), and with antibodies against MMP2 and TIMP3 (Abcam).

    Techniques: Transfection, CCK-8 Assay, Expressing

    Supplementation with pasteurised A. muciniphila or Amuc_1100 blunted tumourigenesis induced by azoxymethane (AOM)/DSS. (A) Weight changes are expressed as the mean change from the starting weight. (B) DAI, (C) spleen weight, (D) tumour development, number (E) and tumour area (F) were analysed. (G) Representative histological images of colon tissues by H E staining. Scale bars, 200 µm. IHC staining and quantitation of γH2AX (8 weeks, H), cleaved-caspase 3 (23 weeks, I) and Ki67 (23 weeks, J) in the proximal colon. Scale bars, 50 µm. Data are presented as the mean±SEM and were analysed by ordinary one-way ANOVA with Tukey’s multiple comparisons. *P

    Journal: Gut

    Article Title: A purified membrane protein from Akkermansia muciniphila or the pasteurised bacterium blunts colitis associated tumourigenesis by modulation of CD8+ T cells in mice

    doi: 10.1136/gutjnl-2019-320105

    Figure Lengend Snippet: Supplementation with pasteurised A. muciniphila or Amuc_1100 blunted tumourigenesis induced by azoxymethane (AOM)/DSS. (A) Weight changes are expressed as the mean change from the starting weight. (B) DAI, (C) spleen weight, (D) tumour development, number (E) and tumour area (F) were analysed. (G) Representative histological images of colon tissues by H E staining. Scale bars, 200 µm. IHC staining and quantitation of γH2AX (8 weeks, H), cleaved-caspase 3 (23 weeks, I) and Ki67 (23 weeks, J) in the proximal colon. Scale bars, 50 µm. Data are presented as the mean±SEM and were analysed by ordinary one-way ANOVA with Tukey’s multiple comparisons. *P

    Article Snippet: The sections were then blocked and stained with CD8a, F4/80, cleaved-caspase 3, γH2AX or Ki67 antibodies, followed by corresponding secondary antibody and a Streptavidin Biotin Complex kit (Boster BioEngineering, Wuhan, China).

    Techniques: Staining, Immunohistochemistry, Quantitation Assay