Journal: Therapeutics and Clinical Risk Management

Article Title: Small bowel gastrointestinal stromal tumor: a retrospective study of 32 cases at a single center and review of the literature

doi: 10.2147/TCRM.S167248

Figure Lengend Snippet: Ileal GISTs and immunohistochemistry. Notes: A 55-year-old male with an ileal GISTs presented with melena. ( A1 ) Axial plain CT image does not reveal the primary tumor due to polymorphous intestine. ( A2 ) Axial contrast-enhanced CT image shows a 1.3×1.8 cm 2 tumor (arrow) with marked enhancement in the arterial phase. ( A3 ) DBE shows the intraluminal component of the tumor with engorged vessels. ( A4 ) Gross appearance of the operative specimen shows the extraluminal component of the tumor (arrow). ( B1 ) Histopathology shows the tumor cells are composed of spindle cells with a low mitotic count (≤5 mitoses/50 HPFs; HE staining). Tumor cells are strongly positive for CD117 ( B2 ), CD34 ( B3 ), and DOG1 ( B4 ). Tumor cells are negative for SMA ( C1 ), which is positive in vascular wall. S-100 ( C2 ) and Desmin ( C3 ) are negative in tumor cells. Ki-67 ( C4 ) is positive in some of the tumor nuclei (global index 2%). (Original magnification: B1 ×100; B2 – C4 ×200). Abbreviations: GISTs, gastrointestinal stromal tumors; CT, computed tomography; DBE, double-balloon enteroscopy; HPFs, high-power fields; HE, hematoxylin and eosin; SMA, smooth muscle actin.

Article Snippet: The routine diagnostic immunohistochemistry technique used a panel of seven primary antibodies purchased from Boster Biological Technology (Wuhan, China), which included a mouse anti-CD117 monoclonal antibody used at 1:100 dilution, a rabbit anti-CD34 polyclonal antibody used at 1:200 dilution, a mouse anti-DOG1 monoclonal antibody used at 1:200 dilution, a mouse anti-SMA monoclonal antibody used at 1:150 dilution, a mouse anti-S100 monoclonal antibody used at 1:50 dilution, a mouse anti-desmin monoclonal antibody used at 1:100 dilution, and a rabbit anti-Ki-67 monoclonal antibody used at 1:100 dilution.

Techniques: Immunohistochemistry, Histopathology, Staining, Computed Tomography

Journal: Oncology Research

Article Title: Long Noncoding RNA Taurine-Upregulated Gene 1 Promotes Cell Proliferation and Invasion in Gastric Cancer via Negatively Modulating miRNA-145-5p

doi: 10.3727/096504016X14783677992682

Figure Lengend Snippet: Effect of TUG1 knockdown on GC cell proliferation and invasion in vitro. (A) qRT-PCR was performed to evaluate the expression of TUG1 in GC cell lines (BGC-823 and SGC-7901) and nonmalignant gastric epithelial cell line (GES-1). GAPDH was considered as the endogenous control. (B) The expression of TUG1 was analyzed in BGC-823 and SGC-7901 cells transfected with si-TUG1 or si-NC. GAPDH was considered as the endogenous control. Cell viability in BGC-823 (C) and SGC-7901 (D) cells transfected with si-TUG1 or si-NC was assessed by MTT assay at 24, 48, 72, and 96 h. Cell invasiveness in BGC-823 (E) and SGC-7901 (F) cells transfected with si-TUG1 or si-NC was detected by Transwell invasion assay. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The tissue sections were then incubated in 3% H 2 O 2 solution for 10 min to block endogenous peroxidase activity and incubated with 1:50 diluted Ki-67 antibody (Boster Biological Technology, Ltd., Wuhan, P.R.

Techniques: In Vitro, Quantitative RT-PCR, Expressing, Transfection, MTT Assay, Transwell Invasion Assay

Journal: Oncology Research

Article Title: Long Noncoding RNA Taurine-Upregulated Gene 1 Promotes Cell Proliferation and Invasion in Gastric Cancer via Negatively Modulating miRNA-145-5p

doi: 10.3727/096504016X14783677992682

Figure Lengend Snippet: Effect of TUG1 knockdown on GC cell growth in vivo. Xenograft model was applied by injecting si-NC- or si-TUG1-transfected SGC-7901 cells into nude mice. (A) The image of tumor xenografts after 24 days of incubation. (B) Tumor volume was monitored and calculated every 4 days for 24 days after injection. (C) Tumor xenograft weight was measured at 24 days after injection. (E) Immunohistochemical analysis of proliferating cell nuclear antigen (Ki-67) protein expression in GC tissue sections from five randomly selected fields. Upper: H&E staining; lower: immunostaining (200×). * p < 0.05, ** p < 0.01.

Article Snippet: The tissue sections were then incubated in 3% H 2 O 2 solution for 10 min to block endogenous peroxidase activity and incubated with 1:50 diluted Ki-67 antibody (Boster Biological Technology, Ltd., Wuhan, P.R.

Techniques: In Vivo, Transfection, Incubation, Injection, Immunohistochemical staining, Expressing, Staining, Immunostaining

Journal: OncoTargets and therapy

Article Title: Aloperine executes antitumor effects through the induction of apoptosis and cell cycle arrest in prostate cancer in vitro and in vivo

doi: 10.2147/OTT.S165262

Figure Lengend Snippet: Aloperine suppressed PC3 xenograft tumor growth in vivo. ( A and B ) PC3 cells were injected into the right flank of 6-week-old male, nude mice. Subcutaneous tumors were formed in nude mice after treatment with either PBS or aloperine (30 mg/kg) for 28 days. ( C ) Tumor volume of aloperine-treated PC3 cells at the indicated time ( *P <0.05 vs vehicle, n=5). ( D ) Histograms describing the mean tumor weights of each group ( *P <0.05 vs vehicle, n=5). ( E ) Mice weight at the indicated time. ( F ) Immunohistochemical staining of Ki-67 and TUNEL assay in the endpoint tumors. ( G and H ) Quantitative analysis of Ki-67-positive and TUNEL-positive cells in tumor tissues of different groups ( *P <0.05 vs vehicle, n=5). Abbreviation: TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling.

Article Snippet: The tumor tissues (both treatment and control group) were stained with antibody against Ki-67 (1:100; Boster Biological Technology, Ltd., Wuhan, People’s Republic of China) to evaluate the proliferation rate of PCa cells in vivo.

Techniques: In Vivo, Injection, Immunohistochemical staining, Staining, TUNEL Assay, End Labeling

Journal: Journal of Translational Medicine

Article Title: Targeting therapy of hepatocellular carcinoma with doxorubicin prodrug PDOX increases anti-metastatic effect and reduces toxicity: a preclinical study

doi: 10.1186/1479-5876-11-192

Figure Lengend Snippet: Immunohistochemical studies on key molecules in tumor growth and metastases. C1, D1 & P1 : the cytoplasmic expression of Cat B in 33 tumors was similar among the 3 groups. C2 , D2 & P2 : The Ki-67 positive rate in the Control group (C2 , 77.1% ) was significantly higher than those in the DOX group (D2 , 72.3% ) and the PDOX group (P2 , 61.6% ) (Control vs DOX: P < 0.05, Control vs PDOX: P < 0.05, DOX vs PDOX: P < 0.05). C3 , D3 & P3 : The CD34 positive micro-vessels density was similar among the 3 groups. C4 , D4 & P4 : The rates of VEGF positive cells were similar among 3 groups. C5 , D5 & P5 : The positive expression of E-Cadherin was similar among the 3 groups. C6 , D6 & P6 : The D2-40 positive lymph vessels were also similar among the 3 groups. C : Control; D : DOX; P : PDOX. 400×, scale bar = 20 μm.

Article Snippet: The primary antibodies for Cat B (Cat No 3190–100, BioVision, CA, USA, dilution 1:200), Ki-67 (MAB-0129, Maxim-Bio Co, CHN, Working solution), CD34 (BA0532, WuHan Boster Bio-Engineering Co, CHN, dilution 1:100), VEGF (RB-9031, Maxim-Bio Co, CHN, Working solution), E-cadherin (MAB-0589, Maxim-Bio Co, CHN, Working solution) and D2-40 (AM0103, Ascend Biotechnology Co, CHN, Working solution) were prepared and incubated with the slides for 2 h in a moist chamber.

Techniques: Immunohistochemical staining, Expressing

Journal: Journal of Translational Medicine

Article Title: Targeting therapy of hepatocellular carcinoma with doxorubicin prodrug PDOX increases anti-metastatic effect and reduces toxicity: a preclinical study

doi: 10.1186/1479-5876-11-192

Figure Lengend Snippet: Immunohistochemical analysis

Article Snippet: The primary antibodies for Cat B (Cat No 3190–100, BioVision, CA, USA, dilution 1:200), Ki-67 (MAB-0129, Maxim-Bio Co, CHN, Working solution), CD34 (BA0532, WuHan Boster Bio-Engineering Co, CHN, dilution 1:100), VEGF (RB-9031, Maxim-Bio Co, CHN, Working solution), E-cadherin (MAB-0589, Maxim-Bio Co, CHN, Working solution) and D2-40 (AM0103, Ascend Biotechnology Co, CHN, Working solution) were prepared and incubated with the slides for 2 h in a moist chamber.

Techniques: Immunohistochemical staining

Journal: International Journal of Medical Sciences

Article Title: MicroRNA-218 Enhances the Radiosensitivity of Human Cervical Cancer via Promoting Radiation Induced Apoptosis

doi: 10.7150/ijms.8880

Figure Lengend Snippet: Combination of miR-218 overexpression and radiation suppressed tumor growth and promoted apoptosis in vivo. (A) with stable transfection, miR-218 was continuously overexpressed in Hela cells; (B) abundant miR-218 increased cellular radiosensitivity of HeLa cells (P<0.01 for 2 Gy and P<0.001 for 4 Gy); (C) combination of miR-218 overexpression and radiation significantly suppressed the growth of HeLa xenograft (NS: not significant; **: P<0.01; ***: P<0.001); (D) combination of miR-218 overexpression and radiation inhibited the expression of Ki 67 and induced more cleaved caspase 3.

Article Snippet: In brief, after rehydration and antigen retrieval, the slides were incubated with primary antibodies: anti-Ki 67 (1:200, Boster Biotech Company, Wuhan, China), anti-cleaved caspase 3 (1:200, Cell Signaling Technology).

Techniques: Over Expression, In Vivo, Stable Transfection, Expressing

Journal: World Journal of Surgical Oncology

Article Title: Effect of Notch1 signaling on cellular proliferation and apoptosis in human laryngeal carcinoma

doi: 10.1186/s12957-022-02728-6

Figure Lengend Snippet: Immunohistochemical analysis of Notch1-, Ki-67-, and TUNEL-positive cells in LSCC tissues. a Notch1 was observed in the cell membranes and cytoplasm of cancer cells (×400). b Ki-67 was found in the nuclei of neoplastic cells (×400). c Apoptotic cells were assessed by the TUNEL method (×400)

Article Snippet: The following primary antibodies were incubated with the tissue sections overnight at 4 °C: rabbit anti-Notch1 monoclonal antibodies (Epitomics, Inc., Burlingame, CA, USA) (1:100 dilution) and rabbit anti-Ki-67 polyclonal antibodies (BA1508, Wuhan Boster, China) (1:800 dilution).

Techniques: Immunohistochemical staining, TUNEL Assay

Journal: World Journal of Surgical Oncology

Article Title: Effect of Notch1 signaling on cellular proliferation and apoptosis in human laryngeal carcinoma

doi: 10.1186/s12957-022-02728-6

Figure Lengend Snippet: Relationship between proliferative and apoptotic indices based on Notch1 expression in laryngeal squamous cell carcinoma

Article Snippet: The following primary antibodies were incubated with the tissue sections overnight at 4 °C: rabbit anti-Notch1 monoclonal antibodies (Epitomics, Inc., Burlingame, CA, USA) (1:100 dilution) and rabbit anti-Ki-67 polyclonal antibodies (BA1508, Wuhan Boster, China) (1:800 dilution).

Techniques: Expressing