anti kchip2  (Alomone Labs)

Journal: PLOS ONE

Article Title: Obesity-induced activation of NADPH oxidase 2 prolongs cardiac repolarization via inhibiting K + currents

doi: 10.1371/journal.pone.0316701

Figure Lengend Snippet: A: Outward K + currents recorded from control (black) and HFD (red) mouse ventricular myocytes were evoked during 300-ms depolarizing voltage steps. B: Voltage relationship (I-V curves) in control and HFD mice. C: I peak at +70 mV in control and HFD mice. D: Representative I to recorded from ventricular myocytes of control (black) and HFD (red) mice. E: Voltage relationship (I-V curves) in the two groups. F: I to at +70 mV in the two groups. G, H and I: Steady-state activation (SSA), steady-state inactivation (SSI), and recovery from inactivation (RFI) curves of I to . J: Voltage relationship (I-V curves) of I K,slow in the two groups. K: I K,slow at +70 mV in the two groups. L: Analyzed fast (lower series) and slow (upper series) inactivation time constants (τ values) from currents. M: Representative Western blots of K V 4.2, KChIP2 and K V 1.5. N: Quantification of K V 4.2. O: Quantification of KChIP2. P: Quantification of K V 1.5. for A-L, n = 8 cells; three or four hearts per group. for M-P, n = 4 animals per group. * p <0.05, ** p <0.01.

Article Snippet: Anti-K V 4.2 (APC-023), anti-KChIP2 (APC-142), anti-K ir 2.1 (APC-026), and anti-K V 1.5 (APC-004, Alomone); anti-Nox2 (Proteintech); and anti-tubulin (Immunoway) were used.

Techniques: Control, Activation Assay, Western Blot

Journal: Scientific Reports

Article Title: The Kv4 potassium channel modulator NS5806 attenuates cardiac hypertrophy in vivo and in vitro

doi: 10.1038/s41598-024-70962-x

Figure Lengend Snippet: Effects of NS5806 on I to channel-related gene and protein expression. ( A – C ) Quantification of the mRNA levels of Kv4.2 ( A ), KChIP2 ( B ) and DPP6 ( C ). GAPDH served as an internal control (n = 9 in each group). ( D ) Representative immunoblots. Original blots are presented in Supplementary Figure . ( E – G ) Summary data of Kv4.2 ( E ), KChIP2 ( F ) and DPP6 ( G ) protein levels. GAPDH served as an internal control (n = 5 in each group). * P < 0.05, ** P < 0.01 versus the Sham + Vehicle group. # P < 0.05, ## P < 0.01 versus TAC + Vehicle.

Article Snippet: Primary antibodies against Kv4.2 (1:500, 75–016, NeuroMab, USA), KChIP2 (1:500, DF14620, Affinity, China), DPP6 (1:200, APC-146, Alomone, Israel) and GAPDH (1:10,000, 10494-1-AP, Proteintech, China) were incubated at 4 °C overnight.

Techniques: Expressing, Control, Western Blot

Journal: Scientific Reports

Article Title: The Kv4 potassium channel modulator NS5806 attenuates cardiac hypertrophy in vivo and in vitro

doi: 10.1038/s41598-024-70962-x

Figure Lengend Snippet: Effects of NS5806 on I to channel-related gene and protein expression. ( A – C ) Quantification of the mRNA levels of Kv4.2 ( A ), KChIP2 ( B ) and DPP6 ( C ). GAPDH served as an internal control (n = 9 in each group). ( D ) Representative immunoblots. Original blots are presented in Supplementary Figure . ( E – G ) Summary data of Kv4.2 ( E ), KChIP2 ( F ) and DPP6 ( G ) protein levels. GAPDH served as an internal control (n = 5 in each group). * P < 0.05, ** P < 0.01 versus the Sham + Vehicle group. # P < 0.05, ## P < 0.01 versus TAC + Vehicle.

Article Snippet: Primary antibodies against Kv4.2 (1:500, 75–016, NeuroMab, USA), KChIP2 (1:500, DF14620, Affinity, China), DPP6 (1:200, APC-146, Alomone, Israel) and GAPDH (1:10,000, 10494-1-AP, Proteintech, China) were incubated at 4 °C overnight.

Techniques: Expressing, Control, Western Blot