anti kca2 3 atto 594  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Alomone Labs anti kca2 3 atto 594
    Expression of CD140α, CD44, CD34 and <t>SK3</t> cell surface markers in iMSCs, fibroblasts, and PDGFRα-positive cells. Flow cytometry analysis detected the expression of CD140α (( A ). cell count; ( B) . mean fluorescence intensity), CD44 (( C ). cell count; ( D ). mean fluorescence intensity), CD34 (( E ). cell count; ( F ). mean fluorescence intensity), and SK3 (( G ). cell count; ( H ). mean fluorescence intensity) in iMSCs, fibroblasts, and PDGFRα-positive ( n = 3, * p
    Anti Kca2 3 Atto 594, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kca2 3 atto 594/product/Alomone Labs
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti kca2 3 atto 594 - by Bioz Stars, 2022-08
    94/100 stars

    Images

    1) Product Images from "Differentiated PDGFRα-Positive Cells: A Novel In-Vitro Model for Functional Studies of Neuronal Nitric Oxide Synthase"

    Article Title: Differentiated PDGFRα-Positive Cells: A Novel In-Vitro Model for Functional Studies of Neuronal Nitric Oxide Synthase

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22073514

    Expression of CD140α, CD44, CD34 and SK3 cell surface markers in iMSCs, fibroblasts, and PDGFRα-positive cells. Flow cytometry analysis detected the expression of CD140α (( A ). cell count; ( B) . mean fluorescence intensity), CD44 (( C ). cell count; ( D ). mean fluorescence intensity), CD34 (( E ). cell count; ( F ). mean fluorescence intensity), and SK3 (( G ). cell count; ( H ). mean fluorescence intensity) in iMSCs, fibroblasts, and PDGFRα-positive ( n = 3, * p
    Figure Legend Snippet: Expression of CD140α, CD44, CD34 and SK3 cell surface markers in iMSCs, fibroblasts, and PDGFRα-positive cells. Flow cytometry analysis detected the expression of CD140α (( A ). cell count; ( B) . mean fluorescence intensity), CD44 (( C ). cell count; ( D ). mean fluorescence intensity), CD34 (( E ). cell count; ( F ). mean fluorescence intensity), and SK3 (( G ). cell count; ( H ). mean fluorescence intensity) in iMSCs, fibroblasts, and PDGFRα-positive ( n = 3, * p

    Techniques Used: Expressing, Flow Cytometry, Cell Counting, Fluorescence

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Alomone Labs anti kca2 3 atto 594
    Expression of CD140α, CD44, CD34 and <t>SK3</t> cell surface markers in iMSCs, fibroblasts, and PDGFRα-positive cells. Flow cytometry analysis detected the expression of CD140α (( A ). cell count; ( B) . mean fluorescence intensity), CD44 (( C ). cell count; ( D ). mean fluorescence intensity), CD34 (( E ). cell count; ( F ). mean fluorescence intensity), and SK3 (( G ). cell count; ( H ). mean fluorescence intensity) in iMSCs, fibroblasts, and PDGFRα-positive ( n = 3, * p
    Anti Kca2 3 Atto 594, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kca2 3 atto 594/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kca2 3 atto 594 - by Bioz Stars, 2022-08
    94/100 stars
      Buy from Supplier

    93
    Alomone Labs sk3 antibody
    Proposed mechanism demonstrating the interaction between the lipid-raft associated <t>Orai1/TRPC1/SK3</t> channel complex and EGFR signaling pathway in colon cancer cell migration This model suggests three positive feedback loops: 1) STIM1 following its phosphorylation by EGF stimulation and Akt, is the trigger of SOCE and promotes migration mediated by the translocation of TRPC1 and Orai1 into lipid rafts where SK3 is concentrated. 2) The Orai1/TRPC1/SK3 channel complex promotes SOCE, which enhances P-Akt leading to the phosphorylation of STIM1, that may promote SOCE and 3) P-Akt activates Rac1, which enhances SOCE and in turn P-Akt. These loops operated toward the same goal: enhancing SOCE and SK3-dependent cell migration.
    Sk3 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sk3 antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sk3 antibody - by Bioz Stars, 2022-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    Expression of CD140α, CD44, CD34 and SK3 cell surface markers in iMSCs, fibroblasts, and PDGFRα-positive cells. Flow cytometry analysis detected the expression of CD140α (( A ). cell count; ( B) . mean fluorescence intensity), CD44 (( C ). cell count; ( D ). mean fluorescence intensity), CD34 (( E ). cell count; ( F ). mean fluorescence intensity), and SK3 (( G ). cell count; ( H ). mean fluorescence intensity) in iMSCs, fibroblasts, and PDGFRα-positive ( n = 3, * p

    Journal: International Journal of Molecular Sciences

    Article Title: Differentiated PDGFRα-Positive Cells: A Novel In-Vitro Model for Functional Studies of Neuronal Nitric Oxide Synthase

    doi: 10.3390/ijms22073514

    Figure Lengend Snippet: Expression of CD140α, CD44, CD34 and SK3 cell surface markers in iMSCs, fibroblasts, and PDGFRα-positive cells. Flow cytometry analysis detected the expression of CD140α (( A ). cell count; ( B) . mean fluorescence intensity), CD44 (( C ). cell count; ( D ). mean fluorescence intensity), CD34 (( E ). cell count; ( F ). mean fluorescence intensity), and SK3 (( G ). cell count; ( H ). mean fluorescence intensity) in iMSCs, fibroblasts, and PDGFRα-positive ( n = 3, * p

    Article Snippet: Cells were then washed with PBS for 10 min and incubated with the following primary antibodies in a humidified chamber overnight at 4 °C: anti-CD34 (ab81289; Abcam, Cambridge, UK), anti-CD44 (BD Pharmingen, San Diego, CA, USA), anti-KCa2.3-ATTO-594 (SK3; Alomone Labs, Jerusalem, Israel), anti-PDGFRα (ab234965; Abcam, Cambridge, UK), and anti-nNOS (3G6B10; Invitrogen Carlsbad, CA, USA).

    Techniques: Expressing, Flow Cytometry, Cell Counting, Fluorescence

    Proposed mechanism demonstrating the interaction between the lipid-raft associated Orai1/TRPC1/SK3 channel complex and EGFR signaling pathway in colon cancer cell migration This model suggests three positive feedback loops: 1) STIM1 following its phosphorylation by EGF stimulation and Akt, is the trigger of SOCE and promotes migration mediated by the translocation of TRPC1 and Orai1 into lipid rafts where SK3 is concentrated. 2) The Orai1/TRPC1/SK3 channel complex promotes SOCE, which enhances P-Akt leading to the phosphorylation of STIM1, that may promote SOCE and 3) P-Akt activates Rac1, which enhances SOCE and in turn P-Akt. These loops operated toward the same goal: enhancing SOCE and SK3-dependent cell migration.

    Journal: Oncotarget

    Article Title: SK3/TRPC1/Orai1 complex regulates SOCE-dependent colon cancer cell migration: a novel opportunity to modulate anti-EGFR mAb action by the alkyl-lipid Ohmline

    doi: 10.18632/oncotarget.8786

    Figure Lengend Snippet: Proposed mechanism demonstrating the interaction between the lipid-raft associated Orai1/TRPC1/SK3 channel complex and EGFR signaling pathway in colon cancer cell migration This model suggests three positive feedback loops: 1) STIM1 following its phosphorylation by EGF stimulation and Akt, is the trigger of SOCE and promotes migration mediated by the translocation of TRPC1 and Orai1 into lipid rafts where SK3 is concentrated. 2) The Orai1/TRPC1/SK3 channel complex promotes SOCE, which enhances P-Akt leading to the phosphorylation of STIM1, that may promote SOCE and 3) P-Akt activates Rac1, which enhances SOCE and in turn P-Akt. These loops operated toward the same goal: enhancing SOCE and SK3-dependent cell migration.

    Article Snippet: Double staining was performed by incubation first with caveolin-1, and then with SK3 antibody (Anti-SK3-ATTO-594, Alomone Lab).

    Techniques: Migration, Translocation Assay

    STIM1 activation, triggered by Ca2+ store depletion, recruits an Orai1/TRPC1 complex into lipid-rafts containing SK3 channels A. Left panel, Immunoblots representing membrane fractionation, on a sucrose gradient of cell lysate in control condition. SK3 is exclusively located into lipid-rafts whereas calcium channels Orai1 and TRPC1 are found outside lipid-rafts. Right panel, representative confocal images of SK3 and Caveolin-1 staining in HCT-116 cells showing immunocolocalization of SK3 and Caveolin-1. B. Depletion of intracellular calcium store by Tg induces the translocation of calcium channels Orai1 and TRPC1 into lipid-rafts whereas SK3 is always in lipid-rafts. Immunoblots representing membrane fractionation, on a sucrose gradient, of cells treated with 5μM Tg for 20 min. C. TRPC1, Orai1, STIM1 and caveolin-1 form a lipid-raft complex in HCT-116 after Ca 2+ store depletion. Immunoblots depict co-immunoprecipitation between STIM1, TRPC1, Orai1 and Caveolin-1 before and after Ca 2+ store depletion.

    Journal: Oncotarget

    Article Title: SK3/TRPC1/Orai1 complex regulates SOCE-dependent colon cancer cell migration: a novel opportunity to modulate anti-EGFR mAb action by the alkyl-lipid Ohmline

    doi: 10.18632/oncotarget.8786

    Figure Lengend Snippet: STIM1 activation, triggered by Ca2+ store depletion, recruits an Orai1/TRPC1 complex into lipid-rafts containing SK3 channels A. Left panel, Immunoblots representing membrane fractionation, on a sucrose gradient of cell lysate in control condition. SK3 is exclusively located into lipid-rafts whereas calcium channels Orai1 and TRPC1 are found outside lipid-rafts. Right panel, representative confocal images of SK3 and Caveolin-1 staining in HCT-116 cells showing immunocolocalization of SK3 and Caveolin-1. B. Depletion of intracellular calcium store by Tg induces the translocation of calcium channels Orai1 and TRPC1 into lipid-rafts whereas SK3 is always in lipid-rafts. Immunoblots representing membrane fractionation, on a sucrose gradient, of cells treated with 5μM Tg for 20 min. C. TRPC1, Orai1, STIM1 and caveolin-1 form a lipid-raft complex in HCT-116 after Ca 2+ store depletion. Immunoblots depict co-immunoprecipitation between STIM1, TRPC1, Orai1 and Caveolin-1 before and after Ca 2+ store depletion.

    Article Snippet: Double staining was performed by incubation first with caveolin-1, and then with SK3 antibody (Anti-SK3-ATTO-594, Alomone Lab).

    Techniques: Activation Assay, Western Blot, Fractionation, Staining, Translocation Assay, Immunoprecipitation

    Ohmline as a new personalized treatment strategy to decrease P-Akt and therefore modulate the effects of Anti-EGFR mAbs A. Disrupting lipid-rafts with the alkyl-lipid Ohmline allows SK3 to re-translocate outside away from lipid-rafts without modifying the localization of calcium channels whereas the co-treatment Ohmline/Tg the translocation of calcium channels into lipid-raft. Immunoblots representing membrane fractionation, on a sucrose gradient, of cells treated with Ohmline alone (middle panel) or associated with 5μM Tg for 20 min (right panel). B. Left panel, Dissociation of the lipid-raft Orai1/TRPC1/SK3 channel complex by Ohmline decreased Ca 2+ entry evoked by Tg. Fluorescence measurement and relative fluorescence of Ca 2+ entry after intracellular calcium store depletion by Tg in cells treated 24h with Ohmline. Data represent means ± SEM. *p

    Journal: Oncotarget

    Article Title: SK3/TRPC1/Orai1 complex regulates SOCE-dependent colon cancer cell migration: a novel opportunity to modulate anti-EGFR mAb action by the alkyl-lipid Ohmline

    doi: 10.18632/oncotarget.8786

    Figure Lengend Snippet: Ohmline as a new personalized treatment strategy to decrease P-Akt and therefore modulate the effects of Anti-EGFR mAbs A. Disrupting lipid-rafts with the alkyl-lipid Ohmline allows SK3 to re-translocate outside away from lipid-rafts without modifying the localization of calcium channels whereas the co-treatment Ohmline/Tg the translocation of calcium channels into lipid-raft. Immunoblots representing membrane fractionation, on a sucrose gradient, of cells treated with Ohmline alone (middle panel) or associated with 5μM Tg for 20 min (right panel). B. Left panel, Dissociation of the lipid-raft Orai1/TRPC1/SK3 channel complex by Ohmline decreased Ca 2+ entry evoked by Tg. Fluorescence measurement and relative fluorescence of Ca 2+ entry after intracellular calcium store depletion by Tg in cells treated 24h with Ohmline. Data represent means ± SEM. *p

    Article Snippet: Double staining was performed by incubation first with caveolin-1, and then with SK3 antibody (Anti-SK3-ATTO-594, Alomone Lab).

    Techniques: Translocation Assay, Western Blot, Fractionation, Fluorescence

    Migration of colon cancer cells HCT-116 is dependent on calcium-activated potassium channel SK3 and SOCE A. SK3 channel is involved in HCT-116 cell migration. Histograms showing HCT-116 cell migration transfected for 48h with siSK3 or treated with Apamin. The normalized cell number corresponds to the ratio of total number of migrating cells in presence of drugs/total number of migrating cells in control experiments. Results are expressed as mean ± SEM. **p

    Journal: Oncotarget

    Article Title: SK3/TRPC1/Orai1 complex regulates SOCE-dependent colon cancer cell migration: a novel opportunity to modulate anti-EGFR mAb action by the alkyl-lipid Ohmline

    doi: 10.18632/oncotarget.8786

    Figure Lengend Snippet: Migration of colon cancer cells HCT-116 is dependent on calcium-activated potassium channel SK3 and SOCE A. SK3 channel is involved in HCT-116 cell migration. Histograms showing HCT-116 cell migration transfected for 48h with siSK3 or treated with Apamin. The normalized cell number corresponds to the ratio of total number of migrating cells in presence of drugs/total number of migrating cells in control experiments. Results are expressed as mean ± SEM. **p

    Article Snippet: Double staining was performed by incubation first with caveolin-1, and then with SK3 antibody (Anti-SK3-ATTO-594, Alomone Lab).

    Techniques: Migration, Transfection