Journal: Frontiers in Immunology

Article Title: Human seminal plasma stimulates the migration of CD11c+ mononuclear phagocytes to the apical side of the colonic epithelium without altering the junctional complexes in an ex vivo human intestinal model

doi: 10.3389/fimmu.2023.1133886

Figure Lengend Snippet: HSP does not affect colonic barrier integrity in an ex vivo culture model. Expression of JAM-A (red, (A) ) and E-CAD (green, (B) ) on tissues incubated with three HSP pools for 90 minutes, from 14 HIV infected patients (HIV1-pool), 12 HIV negative fertile subjects (Fertile-pool) or 21 randomly selected donors (Random-pool). Tight junctions were stained with the JAM-A FITC conjugated mAb, and adherent junctions with the E-CAD mAb followed by Alexa Fluor 546-conjugated goat anti-mouse IgG1 antibody. DAPI stained the nuclei (blue). 40X objective magnification are shown.

Article Snippet: The primary antibody used were: mouse anti human JAM-A FITC conjugated (clone BV16, Hycult biotech, Uden, NL), mouse anti human E-Cadherin (clone 36/E, BD Bioscience, Franklin Lake, NJ, USA), rabbit anti human CD11c (ab52632, ABCAM, Cambridge, UK), and mouse anti human CD64 (ab140779, ABCAM, Cambridge, UK).

Techniques: Ex Vivo, Expressing, Incubation, Infection, Staining

Journal: Oncotarget

Article Title: Dysregulation of junctional adhesion molecule-A via p63/GATA-3 in head and neck squamous cell carcinoma

doi: 10.18632/oncotarget.8432

Figure Lengend Snippet: ( A ) HNSCC and dysplasia, ( B ) cancer pearl region, ( C ) invasive region, ( D ) metastatic lymph node. Bar: 100 μm. ( E ) Real-time PCR for mRNAs of JAM-A and β-catenin in tonsil and HNSCC-patient tissues. Results are given as means ± SE. ( F ) ELISA for soluble JAM-A in sera of HNSCC patients and healthy control subjects. Results are given as means ±SE. ( F ) p * < 0.01.

Article Snippet: An FITC-conjugated anti-JAM-A antibody was obtained from Hycult biotech (Plymouth Meeting, PA).

Techniques: Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

Journal: Oncotarget

Article Title: Dysregulation of junctional adhesion molecule-A via p63/GATA-3 in head and neck squamous cell carcinoma

doi: 10.18632/oncotarget.8432

Figure Lengend Snippet: Proliferation assay ( D ) Matrigel invasion assay ( E ) and images of scratch wound assay ( F ) of Detroit562 cells transfected with negative control siRNA or JAM-A siRNA. Bars: 100 μm. The results are shown as bar graphs. (D, E, F) p * < 0.01.

Article Snippet: An FITC-conjugated anti-JAM-A antibody was obtained from Hycult biotech (Plymouth Meeting, PA).

Techniques: Proliferation Assay, Invasion Assay, Scratch Wound Assay Assay, Transfection, Negative Control

Journal: Oncotarget

Article Title: Dysregulation of junctional adhesion molecule-A via p63/GATA-3 in head and neck squamous cell carcinoma

doi: 10.18632/oncotarget.8432

Figure Lengend Snippet: Western blotting (A) and immunocytochemical staining (B) for JAM-A and β-catenin and flow cytometry (C) for JAM-A in Detroit562 cells treated with the NF-κB inhibitors IMD-0354, MG-132 and curcumin. Bars: 100 μm

Article Snippet: An FITC-conjugated anti-JAM-A antibody was obtained from Hycult biotech (Plymouth Meeting, PA).

Techniques: Western Blot, Staining, Flow Cytometry

Journal: Oncotarget

Article Title: Dysregulation of junctional adhesion molecule-A via p63/GATA-3 in head and neck squamous cell carcinoma

doi: 10.18632/oncotarget.8432

Figure Lengend Snippet: Bar: 100 μm. Western blotting ( B ) and immunocytochemical staining ( C ) for p63, ΔNp63, JAM-A and β-catenin and flow cytometry ( D ) for JAM-A in Detroit562 cells incubated under hypoxia (2% O 2 ). Bars: 20 μm. ( E ) Real-time PCR for JAM-A mRNA in Detroit562 cells incubated under hypoxia (2% O 2 ). Results are given as means ± SE. p * < 0.01.

Article Snippet: An FITC-conjugated anti-JAM-A antibody was obtained from Hycult biotech (Plymouth Meeting, PA).

Techniques: Western Blot, Staining, Flow Cytometry, Incubation, Real-time Polymerase Chain Reaction

Journal: Oncotarget

Article Title: Dysregulation of junctional adhesion molecule-A via p63/GATA-3 in head and neck squamous cell carcinoma

doi: 10.18632/oncotarget.8432

Figure Lengend Snippet: ( G ) Flow cytometry for JAM-A in Detroit562 cells transfected with siRNAs of p63, ΔNp63 and GATA-3. Bars: 20 μm.

Article Snippet: An FITC-conjugated anti-JAM-A antibody was obtained from Hycult biotech (Plymouth Meeting, PA).

Techniques: Flow Cytometry, Transfection

Journal: Oncotarget

Article Title: Dysregulation of junctional adhesion molecule-A via p63/GATA-3 in head and neck squamous cell carcinoma

doi: 10.18632/oncotarget.8432

Figure Lengend Snippet: ( A ) Images of H.E. and immunocytochemical staining for CK7, p63, ΔNp63, GATA-3, JAM-A and β-catenin in primary cultured cancer cells derived from HNSCC tissue. Bar: 100 μm. Western blotting ( B ) for p63, ΔNp63, GATA-3, JAM-A, β-catenin, occludin and claudin-1, 4, 7 and flow cytometry ( C ) for JAM-A HNSCC in primary cultured cancer cells transfected with siRNAs of p63, ΔNp63, GATA-3 and JAM-A.

Article Snippet: An FITC-conjugated anti-JAM-A antibody was obtained from Hycult biotech (Plymouth Meeting, PA).

Techniques: Staining, Cell Culture, Derivative Assay, Western Blot, Flow Cytometry, Transfection