Anti Islet 1, supplied by Epitomics, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Islet-1 induces the differentiation of mesenchymal stem cells into cardiomyocyte-like cells through the regulation of Gcn5 and DNMT-1"
Article Title: Islet-1 induces the differentiation of mesenchymal stem cells into cardiomyocyte-like cells through the regulation of Gcn5 and DNMT-1
Journal: Molecular Medicine Reports
Figure Legend Snippet: DNA methylation levels and acetylation levels of the histone H3K9 site in the GATA4 and Nkx2.5 promoter regions during the differentiation process promoted by Islet-1. (A) The detection of methylation levels on the GATA4 promoter (1329–1489 bp) by MSP assay. (B) The detection of the methylation levels at the Nkx2.5 promoter (51–219 bp) by MSP assay. (C) ChIP results demonstrated the levels of histone acetylation on the promoter regions of GATA4 and Nkx2.5. *P
Techniques Used: DNA Methylation Assay, Methylation, MSP Assay, Chromatin Immunoprecipitation
Figure Legend Snippet: Islet-1 induces the differentiation of C3H10T1/2 cells into cardiomyocytes. (A) The morphological alterations in C3H10T1/2 cells transfected with Lv-GFP or Lv-islet-1 were observed under a microscope. Scale bar=100 µm. (B) Expression of cTnT detected by immunofluorescence microscopy. Scale bar=100 µm. (C) Reverse transcription-quantitative polymerase chain reaction detected variations in mRNA expression levels of cardiac-specific transcription factors in C3H10T1/2 cells infected with lentiviral vectors containing Islet-1. *P
Techniques Used: Transfection, Microscopy, Expressing, Immunofluorescence, Real-time Polymerase Chain Reaction, Infection
Figure Legend Snippet: Successful establishment of Islet-1 overexpression model in C3H10T1/2 cells. (A) Fluorescence microscopy. Scale bar=100 µm. (B) Infection efficiency, as GFP detected by flow cytometry, was 91.7%. (C) Islet-1 protein expression detected by western blotting, with β-actin as a loading control. Islet-1, insulin gene enhancer binding protein ISL-1; GFP, green fluorescent protein.
Techniques Used: Over Expression, Fluorescence, Microscopy, Infection, Flow Cytometry, Cytometry, Expressing, Western Blot, Binding Assay
Article Title: Islet ?-, ?-, and ?-Cell Development Is Controlled by the Ldb1 Coregulator, Acting Primarily With the Islet-1 Transcription Factor
Article Snippet: .. The membrane was blocked in PBS/Tween supplemented with 5% nonfat dry milk followed by incubation overnight with anti-Ldb1 (1:2,000; see Supplementary Table 2 for additional antibody sources),