Structured Review

GeneTex anti isl1
tbx1+ cells contribute to LPM-derived cardiac lineages. a – p Representative maximum intensity projections of whole-mount tbx1 :EGFP-expressing embryos counterstained for anti-EGFP and anti-MHC ( n = 3) ( a – h ) or <t>anti-Isl1</t> ( n = 11) ( i – p ) at 26 hpf; lateral views, anterior to the left. a – d tbx1 reporter expression can be detected in the MHC-positive linear heart tube and in the MHC-negative poles at the cardiac inflow and outflow tracts (arrowheads); e – h depicts a 2.25x magnification of the framed area in a – d . tbx1 :EGFP also marks the pharyngeal arches (pa) and endothelial cells of the cranial vasculature (cv) ( d ). i – p tbx1 reporter-expressing cells at the IFT co-express the SHF marker Isl1 (asterisks n , o ); m – p depicts a 3x magnification of the framed area in i – l . q Quantification of tbx1 :EGFP/Isl1 double- compared to Isl1 single-positive cells at the IFT of the linear heart tube, n = 11 individual embryos analyzed, means ± s.d. r Lineage tracing of tbx1 and drl reporter-expressing cells, shown in representative embryos. tbx1:creERT2 ( n = 11) or drl:creERT2 ( n = 3) transgenics, respectively, were crossed to the ubiquitous hsp70l:Switch loxP tracer line, embryos were 4-OHT-induced at 90% epiboly, and heat-shocked at 3 dpf. s-a’ Live SPIM imaging of still hearts of representative lineage-traced and control embryos; maximum intensity projections of ventral views, anterior to the top, dashed outlines mark the heart with the bulbus arteriosus (BA), atrium (A), and ventricle (V). s – u tbx1 : creERT2 lineage tracing ( tbx1 > EGFP) at late gastrulation labels myl7 :DsRed2-expressing cardiomyocytes (arrowheads) in the ventricle and inflow tract of the atrium, and DAR-4M-stained cells (arrowhead) in the BA. v – x drl :creERT2 -mediated lineage tracing ( drl > EGFP) at 90% epiboly marks all cardiomyocytes (arrowheads) in the ventricle and atrium, BA cells (arrowhead), and the endocardium (arrows). y - a ’ tbx1:creERT2;hsp70l:Switch transgenics without 4-OHT treatment and heat-shocked at 3 dpf show no specific EGFP expression (asterisks mark the autofluorescent pigment cell). Scale bars 20 μm ( e – h , m – p ), 100 μm ( a – d , i – l , s – a ’)
Anti Isl1, supplied by GeneTex, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti isl1/product/GeneTex
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
anti isl1 - by Bioz Stars, 2020-09
92/100 stars

Images

1) Product Images from "Continuous addition of progenitors forms the cardiac ventricle in zebrafish"

Article Title: Continuous addition of progenitors forms the cardiac ventricle in zebrafish

Journal: Nature Communications

doi: 10.1038/s41467-018-04402-6

tbx1+ cells contribute to LPM-derived cardiac lineages. a – p Representative maximum intensity projections of whole-mount tbx1 :EGFP-expressing embryos counterstained for anti-EGFP and anti-MHC ( n = 3) ( a – h ) or anti-Isl1 ( n = 11) ( i – p ) at 26 hpf; lateral views, anterior to the left. a – d tbx1 reporter expression can be detected in the MHC-positive linear heart tube and in the MHC-negative poles at the cardiac inflow and outflow tracts (arrowheads); e – h depicts a 2.25x magnification of the framed area in a – d . tbx1 :EGFP also marks the pharyngeal arches (pa) and endothelial cells of the cranial vasculature (cv) ( d ). i – p tbx1 reporter-expressing cells at the IFT co-express the SHF marker Isl1 (asterisks n , o ); m – p depicts a 3x magnification of the framed area in i – l . q Quantification of tbx1 :EGFP/Isl1 double- compared to Isl1 single-positive cells at the IFT of the linear heart tube, n = 11 individual embryos analyzed, means ± s.d. r Lineage tracing of tbx1 and drl reporter-expressing cells, shown in representative embryos. tbx1:creERT2 ( n = 11) or drl:creERT2 ( n = 3) transgenics, respectively, were crossed to the ubiquitous hsp70l:Switch loxP tracer line, embryos were 4-OHT-induced at 90% epiboly, and heat-shocked at 3 dpf. s-a’ Live SPIM imaging of still hearts of representative lineage-traced and control embryos; maximum intensity projections of ventral views, anterior to the top, dashed outlines mark the heart with the bulbus arteriosus (BA), atrium (A), and ventricle (V). s – u tbx1 : creERT2 lineage tracing ( tbx1 > EGFP) at late gastrulation labels myl7 :DsRed2-expressing cardiomyocytes (arrowheads) in the ventricle and inflow tract of the atrium, and DAR-4M-stained cells (arrowhead) in the BA. v – x drl :creERT2 -mediated lineage tracing ( drl > EGFP) at 90% epiboly marks all cardiomyocytes (arrowheads) in the ventricle and atrium, BA cells (arrowhead), and the endocardium (arrows). y - a ’ tbx1:creERT2;hsp70l:Switch transgenics without 4-OHT treatment and heat-shocked at 3 dpf show no specific EGFP expression (asterisks mark the autofluorescent pigment cell). Scale bars 20 μm ( e – h , m – p ), 100 μm ( a – d , i – l , s – a ’)
Figure Legend Snippet: tbx1+ cells contribute to LPM-derived cardiac lineages. a – p Representative maximum intensity projections of whole-mount tbx1 :EGFP-expressing embryos counterstained for anti-EGFP and anti-MHC ( n = 3) ( a – h ) or anti-Isl1 ( n = 11) ( i – p ) at 26 hpf; lateral views, anterior to the left. a – d tbx1 reporter expression can be detected in the MHC-positive linear heart tube and in the MHC-negative poles at the cardiac inflow and outflow tracts (arrowheads); e – h depicts a 2.25x magnification of the framed area in a – d . tbx1 :EGFP also marks the pharyngeal arches (pa) and endothelial cells of the cranial vasculature (cv) ( d ). i – p tbx1 reporter-expressing cells at the IFT co-express the SHF marker Isl1 (asterisks n , o ); m – p depicts a 3x magnification of the framed area in i – l . q Quantification of tbx1 :EGFP/Isl1 double- compared to Isl1 single-positive cells at the IFT of the linear heart tube, n = 11 individual embryos analyzed, means ± s.d. r Lineage tracing of tbx1 and drl reporter-expressing cells, shown in representative embryos. tbx1:creERT2 ( n = 11) or drl:creERT2 ( n = 3) transgenics, respectively, were crossed to the ubiquitous hsp70l:Switch loxP tracer line, embryos were 4-OHT-induced at 90% epiboly, and heat-shocked at 3 dpf. s-a’ Live SPIM imaging of still hearts of representative lineage-traced and control embryos; maximum intensity projections of ventral views, anterior to the top, dashed outlines mark the heart with the bulbus arteriosus (BA), atrium (A), and ventricle (V). s – u tbx1 : creERT2 lineage tracing ( tbx1 > EGFP) at late gastrulation labels myl7 :DsRed2-expressing cardiomyocytes (arrowheads) in the ventricle and inflow tract of the atrium, and DAR-4M-stained cells (arrowhead) in the BA. v – x drl :creERT2 -mediated lineage tracing ( drl > EGFP) at 90% epiboly marks all cardiomyocytes (arrowheads) in the ventricle and atrium, BA cells (arrowhead), and the endocardium (arrows). y - a ’ tbx1:creERT2;hsp70l:Switch transgenics without 4-OHT treatment and heat-shocked at 3 dpf show no specific EGFP expression (asterisks mark the autofluorescent pigment cell). Scale bars 20 μm ( e – h , m – p ), 100 μm ( a – d , i – l , s – a ’)

Techniques Used: Derivative Assay, Expressing, Marker, Imaging, Staining

2) Product Images from "Continuous addition of progenitors forms the cardiac ventricle in zebrafish"

Article Title: Continuous addition of progenitors forms the cardiac ventricle in zebrafish

Journal: Nature Communications

doi: 10.1038/s41467-018-04402-6

tbx1+ cells contribute to LPM-derived cardiac lineages. a – p Representative maximum intensity projections of whole-mount tbx1 :EGFP-expressing embryos counterstained for anti-EGFP and anti-MHC ( n = 3) ( a – h ) or anti-Isl1 ( n = 11) ( i – p ) at 26 hpf; lateral views, anterior to the left. a – d tbx1 reporter expression can be detected in the MHC-positive linear heart tube and in the MHC-negative poles at the cardiac inflow and outflow tracts (arrowheads); e – h depicts a 2.25x magnification of the framed area in a – d . tbx1 :EGFP also marks the pharyngeal arches (pa) and endothelial cells of the cranial vasculature (cv) ( d ). i – p tbx1 reporter-expressing cells at the IFT co-express the SHF marker Isl1 (asterisks n , o ); m – p depicts a 3x magnification of the framed area in i – l . q Quantification of tbx1 :EGFP/Isl1 double- compared to Isl1 single-positive cells at the IFT of the linear heart tube, n = 11 individual embryos analyzed, means ± s.d. r Lineage tracing of tbx1 and drl reporter-expressing cells, shown in representative embryos. tbx1:creERT2 ( n = 11) or drl:creERT2 ( n = 3) transgenics, respectively, were crossed to the ubiquitous hsp70l:Switch loxP tracer line, embryos were 4-OHT-induced at 90% epiboly, and heat-shocked at 3 dpf. s-a’ Live SPIM imaging of still hearts of representative lineage-traced and control embryos; maximum intensity projections of ventral views, anterior to the top, dashed outlines mark the heart with the bulbus arteriosus (BA), atrium (A), and ventricle (V). s – u tbx1 : creERT2 lineage tracing ( tbx1 > EGFP) at late gastrulation labels myl7 :DsRed2-expressing cardiomyocytes (arrowheads) in the ventricle and inflow tract of the atrium, and DAR-4M-stained cells (arrowhead) in the BA. v – x drl :creERT2 -mediated lineage tracing ( drl > EGFP) at 90% epiboly marks all cardiomyocytes (arrowheads) in the ventricle and atrium, BA cells (arrowhead), and the endocardium (arrows). y - a ’ tbx1:creERT2;hsp70l:Switch transgenics without 4-OHT treatment and heat-shocked at 3 dpf show no specific EGFP expression (asterisks mark the autofluorescent pigment cell). Scale bars 20 μm ( e – h , m – p ), 100 μm ( a – d , i – l , s – a ’)
Figure Legend Snippet: tbx1+ cells contribute to LPM-derived cardiac lineages. a – p Representative maximum intensity projections of whole-mount tbx1 :EGFP-expressing embryos counterstained for anti-EGFP and anti-MHC ( n = 3) ( a – h ) or anti-Isl1 ( n = 11) ( i – p ) at 26 hpf; lateral views, anterior to the left. a – d tbx1 reporter expression can be detected in the MHC-positive linear heart tube and in the MHC-negative poles at the cardiac inflow and outflow tracts (arrowheads); e – h depicts a 2.25x magnification of the framed area in a – d . tbx1 :EGFP also marks the pharyngeal arches (pa) and endothelial cells of the cranial vasculature (cv) ( d ). i – p tbx1 reporter-expressing cells at the IFT co-express the SHF marker Isl1 (asterisks n , o ); m – p depicts a 3x magnification of the framed area in i – l . q Quantification of tbx1 :EGFP/Isl1 double- compared to Isl1 single-positive cells at the IFT of the linear heart tube, n = 11 individual embryos analyzed, means ± s.d. r Lineage tracing of tbx1 and drl reporter-expressing cells, shown in representative embryos. tbx1:creERT2 ( n = 11) or drl:creERT2 ( n = 3) transgenics, respectively, were crossed to the ubiquitous hsp70l:Switch loxP tracer line, embryos were 4-OHT-induced at 90% epiboly, and heat-shocked at 3 dpf. s-a’ Live SPIM imaging of still hearts of representative lineage-traced and control embryos; maximum intensity projections of ventral views, anterior to the top, dashed outlines mark the heart with the bulbus arteriosus (BA), atrium (A), and ventricle (V). s – u tbx1 : creERT2 lineage tracing ( tbx1 > EGFP) at late gastrulation labels myl7 :DsRed2-expressing cardiomyocytes (arrowheads) in the ventricle and inflow tract of the atrium, and DAR-4M-stained cells (arrowhead) in the BA. v – x drl :creERT2 -mediated lineage tracing ( drl > EGFP) at 90% epiboly marks all cardiomyocytes (arrowheads) in the ventricle and atrium, BA cells (arrowhead), and the endocardium (arrows). y - a ’ tbx1:creERT2;hsp70l:Switch transgenics without 4-OHT treatment and heat-shocked at 3 dpf show no specific EGFP expression (asterisks mark the autofluorescent pigment cell). Scale bars 20 μm ( e – h , m – p ), 100 μm ( a – d , i – l , s – a ’)

Techniques Used: Derivative Assay, Expressing, Marker, Imaging, Staining

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    GeneTex anti isl1
    tbx1+ cells contribute to LPM-derived cardiac lineages. a – p Representative maximum intensity projections of whole-mount tbx1 :EGFP-expressing embryos counterstained for anti-EGFP and anti-MHC ( n = 3) ( a – h ) or <t>anti-Isl1</t> ( n = 11) ( i – p ) at 26 hpf; lateral views, anterior to the left. a – d tbx1 reporter expression can be detected in the MHC-positive linear heart tube and in the MHC-negative poles at the cardiac inflow and outflow tracts (arrowheads); e – h depicts a 2.25x magnification of the framed area in a – d . tbx1 :EGFP also marks the pharyngeal arches (pa) and endothelial cells of the cranial vasculature (cv) ( d ). i – p tbx1 reporter-expressing cells at the IFT co-express the SHF marker Isl1 (asterisks n , o ); m – p depicts a 3x magnification of the framed area in i – l . q Quantification of tbx1 :EGFP/Isl1 double- compared to Isl1 single-positive cells at the IFT of the linear heart tube, n = 11 individual embryos analyzed, means ± s.d. r Lineage tracing of tbx1 and drl reporter-expressing cells, shown in representative embryos. tbx1:creERT2 ( n = 11) or drl:creERT2 ( n = 3) transgenics, respectively, were crossed to the ubiquitous hsp70l:Switch loxP tracer line, embryos were 4-OHT-induced at 90% epiboly, and heat-shocked at 3 dpf. s-a’ Live SPIM imaging of still hearts of representative lineage-traced and control embryos; maximum intensity projections of ventral views, anterior to the top, dashed outlines mark the heart with the bulbus arteriosus (BA), atrium (A), and ventricle (V). s – u tbx1 : creERT2 lineage tracing ( tbx1 > EGFP) at late gastrulation labels myl7 :DsRed2-expressing cardiomyocytes (arrowheads) in the ventricle and inflow tract of the atrium, and DAR-4M-stained cells (arrowhead) in the BA. v – x drl :creERT2 -mediated lineage tracing ( drl > EGFP) at 90% epiboly marks all cardiomyocytes (arrowheads) in the ventricle and atrium, BA cells (arrowhead), and the endocardium (arrows). y - a ’ tbx1:creERT2;hsp70l:Switch transgenics without 4-OHT treatment and heat-shocked at 3 dpf show no specific EGFP expression (asterisks mark the autofluorescent pigment cell). Scale bars 20 μm ( e – h , m – p ), 100 μm ( a – d , i – l , s – a ’)
    Anti Isl1, supplied by GeneTex, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti isl1/product/GeneTex
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti isl1 - by Bioz Stars, 2020-09
    92/100 stars
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    tbx1+ cells contribute to LPM-derived cardiac lineages. a – p Representative maximum intensity projections of whole-mount tbx1 :EGFP-expressing embryos counterstained for anti-EGFP and anti-MHC ( n = 3) ( a – h ) or anti-Isl1 ( n = 11) ( i – p ) at 26 hpf; lateral views, anterior to the left. a – d tbx1 reporter expression can be detected in the MHC-positive linear heart tube and in the MHC-negative poles at the cardiac inflow and outflow tracts (arrowheads); e – h depicts a 2.25x magnification of the framed area in a – d . tbx1 :EGFP also marks the pharyngeal arches (pa) and endothelial cells of the cranial vasculature (cv) ( d ). i – p tbx1 reporter-expressing cells at the IFT co-express the SHF marker Isl1 (asterisks n , o ); m – p depicts a 3x magnification of the framed area in i – l . q Quantification of tbx1 :EGFP/Isl1 double- compared to Isl1 single-positive cells at the IFT of the linear heart tube, n = 11 individual embryos analyzed, means ± s.d. r Lineage tracing of tbx1 and drl reporter-expressing cells, shown in representative embryos. tbx1:creERT2 ( n = 11) or drl:creERT2 ( n = 3) transgenics, respectively, were crossed to the ubiquitous hsp70l:Switch loxP tracer line, embryos were 4-OHT-induced at 90% epiboly, and heat-shocked at 3 dpf. s-a’ Live SPIM imaging of still hearts of representative lineage-traced and control embryos; maximum intensity projections of ventral views, anterior to the top, dashed outlines mark the heart with the bulbus arteriosus (BA), atrium (A), and ventricle (V). s – u tbx1 : creERT2 lineage tracing ( tbx1 > EGFP) at late gastrulation labels myl7 :DsRed2-expressing cardiomyocytes (arrowheads) in the ventricle and inflow tract of the atrium, and DAR-4M-stained cells (arrowhead) in the BA. v – x drl :creERT2 -mediated lineage tracing ( drl > EGFP) at 90% epiboly marks all cardiomyocytes (arrowheads) in the ventricle and atrium, BA cells (arrowhead), and the endocardium (arrows). y - a ’ tbx1:creERT2;hsp70l:Switch transgenics without 4-OHT treatment and heat-shocked at 3 dpf show no specific EGFP expression (asterisks mark the autofluorescent pigment cell). Scale bars 20 μm ( e – h , m – p ), 100 μm ( a – d , i – l , s – a ’)

    Journal: Nature Communications

    Article Title: Continuous addition of progenitors forms the cardiac ventricle in zebrafish

    doi: 10.1038/s41467-018-04402-6

    Figure Lengend Snippet: tbx1+ cells contribute to LPM-derived cardiac lineages. a – p Representative maximum intensity projections of whole-mount tbx1 :EGFP-expressing embryos counterstained for anti-EGFP and anti-MHC ( n = 3) ( a – h ) or anti-Isl1 ( n = 11) ( i – p ) at 26 hpf; lateral views, anterior to the left. a – d tbx1 reporter expression can be detected in the MHC-positive linear heart tube and in the MHC-negative poles at the cardiac inflow and outflow tracts (arrowheads); e – h depicts a 2.25x magnification of the framed area in a – d . tbx1 :EGFP also marks the pharyngeal arches (pa) and endothelial cells of the cranial vasculature (cv) ( d ). i – p tbx1 reporter-expressing cells at the IFT co-express the SHF marker Isl1 (asterisks n , o ); m – p depicts a 3x magnification of the framed area in i – l . q Quantification of tbx1 :EGFP/Isl1 double- compared to Isl1 single-positive cells at the IFT of the linear heart tube, n = 11 individual embryos analyzed, means ± s.d. r Lineage tracing of tbx1 and drl reporter-expressing cells, shown in representative embryos. tbx1:creERT2 ( n = 11) or drl:creERT2 ( n = 3) transgenics, respectively, were crossed to the ubiquitous hsp70l:Switch loxP tracer line, embryos were 4-OHT-induced at 90% epiboly, and heat-shocked at 3 dpf. s-a’ Live SPIM imaging of still hearts of representative lineage-traced and control embryos; maximum intensity projections of ventral views, anterior to the top, dashed outlines mark the heart with the bulbus arteriosus (BA), atrium (A), and ventricle (V). s – u tbx1 : creERT2 lineage tracing ( tbx1 > EGFP) at late gastrulation labels myl7 :DsRed2-expressing cardiomyocytes (arrowheads) in the ventricle and inflow tract of the atrium, and DAR-4M-stained cells (arrowhead) in the BA. v – x drl :creERT2 -mediated lineage tracing ( drl > EGFP) at 90% epiboly marks all cardiomyocytes (arrowheads) in the ventricle and atrium, BA cells (arrowhead), and the endocardium (arrows). y - a ’ tbx1:creERT2;hsp70l:Switch transgenics without 4-OHT treatment and heat-shocked at 3 dpf show no specific EGFP expression (asterisks mark the autofluorescent pigment cell). Scale bars 20 μm ( e – h , m – p ), 100 μm ( a – d , i – l , s – a ’)

    Article Snippet: Primary antibodies used were anti-MHC (MF20 supernatant, DSHB, 1:50), anti-GFP (Abcam, ab13970, 1:500 or Sigma, G1544, 1:100), and anti-Isl1 (GeneTex, GTX128201, 1:50).

    Techniques: Derivative Assay, Expressing, Marker, Imaging, Staining

    tbx1+ cells contribute to LPM-derived cardiac lineages. a – p Representative maximum intensity projections of whole-mount tbx1 :EGFP-expressing embryos counterstained for anti-EGFP and anti-MHC ( n = 3) ( a – h ) or anti-Isl1 ( n = 11) ( i – p ) at 26 hpf; lateral views, anterior to the left. a – d tbx1 reporter expression can be detected in the MHC-positive linear heart tube and in the MHC-negative poles at the cardiac inflow and outflow tracts (arrowheads); e – h depicts a 2.25x magnification of the framed area in a – d . tbx1 :EGFP also marks the pharyngeal arches (pa) and endothelial cells of the cranial vasculature (cv) ( d ). i – p tbx1 reporter-expressing cells at the IFT co-express the SHF marker Isl1 (asterisks n , o ); m – p depicts a 3x magnification of the framed area in i – l . q Quantification of tbx1 :EGFP/Isl1 double- compared to Isl1 single-positive cells at the IFT of the linear heart tube, n = 11 individual embryos analyzed, means ± s.d. r Lineage tracing of tbx1 and drl reporter-expressing cells, shown in representative embryos. tbx1:creERT2 ( n = 11) or drl:creERT2 ( n = 3) transgenics, respectively, were crossed to the ubiquitous hsp70l:Switch loxP tracer line, embryos were 4-OHT-induced at 90% epiboly, and heat-shocked at 3 dpf. s-a’ Live SPIM imaging of still hearts of representative lineage-traced and control embryos; maximum intensity projections of ventral views, anterior to the top, dashed outlines mark the heart with the bulbus arteriosus (BA), atrium (A), and ventricle (V). s – u tbx1 : creERT2 lineage tracing ( tbx1 > EGFP) at late gastrulation labels myl7 :DsRed2-expressing cardiomyocytes (arrowheads) in the ventricle and inflow tract of the atrium, and DAR-4M-stained cells (arrowhead) in the BA. v – x drl :creERT2 -mediated lineage tracing ( drl > EGFP) at 90% epiboly marks all cardiomyocytes (arrowheads) in the ventricle and atrium, BA cells (arrowhead), and the endocardium (arrows). y - a ’ tbx1:creERT2;hsp70l:Switch transgenics without 4-OHT treatment and heat-shocked at 3 dpf show no specific EGFP expression (asterisks mark the autofluorescent pigment cell). Scale bars 20 μm ( e – h , m – p ), 100 μm ( a – d , i – l , s – a ’)

    Journal: Nature Communications

    Article Title: Continuous addition of progenitors forms the cardiac ventricle in zebrafish

    doi: 10.1038/s41467-018-04402-6

    Figure Lengend Snippet: tbx1+ cells contribute to LPM-derived cardiac lineages. a – p Representative maximum intensity projections of whole-mount tbx1 :EGFP-expressing embryos counterstained for anti-EGFP and anti-MHC ( n = 3) ( a – h ) or anti-Isl1 ( n = 11) ( i – p ) at 26 hpf; lateral views, anterior to the left. a – d tbx1 reporter expression can be detected in the MHC-positive linear heart tube and in the MHC-negative poles at the cardiac inflow and outflow tracts (arrowheads); e – h depicts a 2.25x magnification of the framed area in a – d . tbx1 :EGFP also marks the pharyngeal arches (pa) and endothelial cells of the cranial vasculature (cv) ( d ). i – p tbx1 reporter-expressing cells at the IFT co-express the SHF marker Isl1 (asterisks n , o ); m – p depicts a 3x magnification of the framed area in i – l . q Quantification of tbx1 :EGFP/Isl1 double- compared to Isl1 single-positive cells at the IFT of the linear heart tube, n = 11 individual embryos analyzed, means ± s.d. r Lineage tracing of tbx1 and drl reporter-expressing cells, shown in representative embryos. tbx1:creERT2 ( n = 11) or drl:creERT2 ( n = 3) transgenics, respectively, were crossed to the ubiquitous hsp70l:Switch loxP tracer line, embryos were 4-OHT-induced at 90% epiboly, and heat-shocked at 3 dpf. s-a’ Live SPIM imaging of still hearts of representative lineage-traced and control embryos; maximum intensity projections of ventral views, anterior to the top, dashed outlines mark the heart with the bulbus arteriosus (BA), atrium (A), and ventricle (V). s – u tbx1 : creERT2 lineage tracing ( tbx1 > EGFP) at late gastrulation labels myl7 :DsRed2-expressing cardiomyocytes (arrowheads) in the ventricle and inflow tract of the atrium, and DAR-4M-stained cells (arrowhead) in the BA. v – x drl :creERT2 -mediated lineage tracing ( drl > EGFP) at 90% epiboly marks all cardiomyocytes (arrowheads) in the ventricle and atrium, BA cells (arrowhead), and the endocardium (arrows). y - a ’ tbx1:creERT2;hsp70l:Switch transgenics without 4-OHT treatment and heat-shocked at 3 dpf show no specific EGFP expression (asterisks mark the autofluorescent pigment cell). Scale bars 20 μm ( e – h , m – p ), 100 μm ( a – d , i – l , s – a ’)

    Article Snippet: Primary antibodies used were anti-MHC (MF20 supernatant, DSHB, 1:50), anti-GFP (Abcam, ab13970, 1:500 or Sigma, G1544, 1:100), and anti-Isl1 (GeneTex, GTX128201, 1:50).

    Techniques: Derivative Assay, Expressing, Marker, Imaging, Staining