anti il 4  (Thermo Fisher)


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    Name:
    IL 4 Monoclonal Antibody A155B15C6
    Description:
    IL 4 Monoclonal Antibody for ELISA
    Catalog Number:
    ASC0849
    Price:
    None
    Category:
    Antibodies Secondary Detection Reagents
    Applications:
    Build Your Own Immunoassay|Cell Analysis|ELISA|Protein Assays and Analysis|Protein Biology|Ready-To-Use Immunoassay
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    Structured Review

    Thermo Fisher anti il 4
    Effects of Nup98 on Th17 cell differentiation in AVMC . CVB3 infected CD4 + T cells from peripheral blood in AVMC patients were transfected with Nup98 siRNA or Nup98 cDNA by the Human T Cell Nucleofector Kit and cultured with anti-CD3, anti-CD28, <t>anti-IL-4</t> and anti-IFN-γ for 72 h. (A) Representative pictures of Th17 cell frequencies of CD4 + T cell in pcDNA3.1, NC, pcDNA3.1-Nup98, and siRNA-nup98 groups were listed. (B) The statistical analysis for Th17 frequencies was shown in histogram. (C) The GFP-transfection efficiency was detected by flow cytometry and a typical FACS picture was shown. (D) The mRNA levels of Nup98, RORγT and IL-17 in CD4 + T cells of four groups. (E) The cultural supernatant IL-17 concentration was measured by ELISA. Data are shown as mean ± SEM. * P
    IL 4 Monoclonal Antibody for ELISA
    https://www.bioz.com/result/anti il 4/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti il 4 - by Bioz Stars, 2021-06
    94/100 stars

    Images

    1) Product Images from "Coxsackievirus B3 Directly Induced Th17 Cell Differentiation by Inhibiting Nup98 Expression in Patients with Acute Viral Myocarditis"

    Article Title: Coxsackievirus B3 Directly Induced Th17 Cell Differentiation by Inhibiting Nup98 Expression in Patients with Acute Viral Myocarditis

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2016.00171

    Effects of Nup98 on Th17 cell differentiation in AVMC . CVB3 infected CD4 + T cells from peripheral blood in AVMC patients were transfected with Nup98 siRNA or Nup98 cDNA by the Human T Cell Nucleofector Kit and cultured with anti-CD3, anti-CD28, anti-IL-4 and anti-IFN-γ for 72 h. (A) Representative pictures of Th17 cell frequencies of CD4 + T cell in pcDNA3.1, NC, pcDNA3.1-Nup98, and siRNA-nup98 groups were listed. (B) The statistical analysis for Th17 frequencies was shown in histogram. (C) The GFP-transfection efficiency was detected by flow cytometry and a typical FACS picture was shown. (D) The mRNA levels of Nup98, RORγT and IL-17 in CD4 + T cells of four groups. (E) The cultural supernatant IL-17 concentration was measured by ELISA. Data are shown as mean ± SEM. * P
    Figure Legend Snippet: Effects of Nup98 on Th17 cell differentiation in AVMC . CVB3 infected CD4 + T cells from peripheral blood in AVMC patients were transfected with Nup98 siRNA or Nup98 cDNA by the Human T Cell Nucleofector Kit and cultured with anti-CD3, anti-CD28, anti-IL-4 and anti-IFN-γ for 72 h. (A) Representative pictures of Th17 cell frequencies of CD4 + T cell in pcDNA3.1, NC, pcDNA3.1-Nup98, and siRNA-nup98 groups were listed. (B) The statistical analysis for Th17 frequencies was shown in histogram. (C) The GFP-transfection efficiency was detected by flow cytometry and a typical FACS picture was shown. (D) The mRNA levels of Nup98, RORγT and IL-17 in CD4 + T cells of four groups. (E) The cultural supernatant IL-17 concentration was measured by ELISA. Data are shown as mean ± SEM. * P

    Techniques Used: Cell Differentiation, Infection, Transfection, Cell Culture, Flow Cytometry, Cytometry, FACS, Concentration Assay, Enzyme-linked Immunosorbent Assay

    2) Product Images from "Recent thymic emigrants are biased against the T-helper type 1 and toward the T-helper type 2 effector lineage"

    Article Title: Recent thymic emigrants are biased against the T-helper type 1 and toward the T-helper type 2 effector lineage

    Journal: Blood

    doi: 10.1182/blood-2010-07-299263

    CD4 + RTEs are biased toward the Th2 effector lineage in vitro . (A) CD4 + RTEs and MN T cells were differentiated under Th2 conditions for 5 days and then stained for IL-4. Numbers in quadrants of representative flow cytometry plots show the percentage
    Figure Legend Snippet: CD4 + RTEs are biased toward the Th2 effector lineage in vitro . (A) CD4 + RTEs and MN T cells were differentiated under Th2 conditions for 5 days and then stained for IL-4. Numbers in quadrants of representative flow cytometry plots show the percentage

    Techniques Used: In Vitro, Staining, Flow Cytometry, Cytometry

    3) Product Images from "ILDR2-Fc Is a Novel Regulator of Immune Homeostasis and Inducer of Antigen-Specific Immune Tolerance"

    Article Title: ILDR2-Fc Is a Novel Regulator of Immune Homeostasis and Inducer of Antigen-Specific Immune Tolerance

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700326

    ILDR2-mFc treatment inhibits myelin epitope-specific epitope spreading in the R-EAE model. ( A ) On day 76 postdisease induction, five mice from each treatment group were analyzed for recall responses to spread epitopes, via injection of 10 μg of PLP 178–191 in one ear and MBP 84–104 into the opposite ear. The level of ear swelling was assayed 24 h postchallenge. The data are presented as the mean net ear swelling. ( B ) Recall responses were also carried on splenocytes. On day 76 postdisease induction, total splenocytes were collected from five representative mice from each treatment group and activated in the presence of OVA 323–339 , PLP 139–151 , PLP 178–191 , or MBP 84–104 (20 μg/ml). Cells were pulsed with 1 mCi of tritiated thymidine at 24 h and harvested 72 h postculture set up. Cell proliferation in recall responses was also carried out using splenocytes from day-45 mice ( C ) or IFN-γ, IL-17, and IL-4 secretion was analyzed by LiquiChip following 72 h of culture ( D–F ). One representative experiment of two or three independent experiments is presented for each experimental set. * p
    Figure Legend Snippet: ILDR2-mFc treatment inhibits myelin epitope-specific epitope spreading in the R-EAE model. ( A ) On day 76 postdisease induction, five mice from each treatment group were analyzed for recall responses to spread epitopes, via injection of 10 μg of PLP 178–191 in one ear and MBP 84–104 into the opposite ear. The level of ear swelling was assayed 24 h postchallenge. The data are presented as the mean net ear swelling. ( B ) Recall responses were also carried on splenocytes. On day 76 postdisease induction, total splenocytes were collected from five representative mice from each treatment group and activated in the presence of OVA 323–339 , PLP 139–151 , PLP 178–191 , or MBP 84–104 (20 μg/ml). Cells were pulsed with 1 mCi of tritiated thymidine at 24 h and harvested 72 h postculture set up. Cell proliferation in recall responses was also carried out using splenocytes from day-45 mice ( C ) or IFN-γ, IL-17, and IL-4 secretion was analyzed by LiquiChip following 72 h of culture ( D–F ). One representative experiment of two or three independent experiments is presented for each experimental set. * p

    Techniques Used: Mouse Assay, Injection, Plasmid Purification

    4) Product Images from "An association of Aquaporin-4 with the immunoregulation of liver pathology in mice infected with Schistosoma japonicum"

    Article Title: An association of Aquaporin-4 with the immunoregulation of liver pathology in mice infected with Schistosoma japonicum

    Journal: Parasites & Vectors

    doi: 10.1186/s13071-015-0650-7

    Th2 cell responses are stronger in S. japonicum- infected AQP4 KO mice . Four AQP4 WT or KO mice were randomly chosen and sacrificed at 0, 3, 5, 8 weeks post-infection. (A) FCM analysis of Th2 cell subsets in AQP4 WT and KO mouse splenocytes, mesenteric lymphocytes and hepatocytes. (B) The kinetics of the percentages (gated on CD3 + cells) of Th2 cells in total CD3 + T cells in AQP4 WT and KO mouse spleens, mesenteric lymph nodes and livers. Representative histograms obtained by FCM analysis (C) of mean fluorescence intensity (MFI) of IL-4 expression in Th2 cells (D) . (E) The kinetics of the absolute numbers of Th2 cells in AQP4 WT and KO mouse spleens, mesenteric lymph nodes and livers. Results are expressed as mean ± SD of 8 mice from two independent experiments. # P
    Figure Legend Snippet: Th2 cell responses are stronger in S. japonicum- infected AQP4 KO mice . Four AQP4 WT or KO mice were randomly chosen and sacrificed at 0, 3, 5, 8 weeks post-infection. (A) FCM analysis of Th2 cell subsets in AQP4 WT and KO mouse splenocytes, mesenteric lymphocytes and hepatocytes. (B) The kinetics of the percentages (gated on CD3 + cells) of Th2 cells in total CD3 + T cells in AQP4 WT and KO mouse spleens, mesenteric lymph nodes and livers. Representative histograms obtained by FCM analysis (C) of mean fluorescence intensity (MFI) of IL-4 expression in Th2 cells (D) . (E) The kinetics of the absolute numbers of Th2 cells in AQP4 WT and KO mouse spleens, mesenteric lymph nodes and livers. Results are expressed as mean ± SD of 8 mice from two independent experiments. # P

    Techniques Used: Infection, Mouse Assay, Fluorescence, Expressing

    5) Product Images from "The Role of Hepatic Invariant (I)NKT Cells in Systemic/Local Inflammation and Mortality During Polymicrobial Septic Shock"

    Article Title: The Role of Hepatic Invariant (I)NKT Cells in Systemic/Local Inflammation and Mortality During Polymicrobial Septic Shock

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.0801463

    Anti-CD1d treatment leads to reduced iNKT cell cytokine production after CLP Assessment of the effect of in vivo anti-CD1d antibody pre-treatment (as opposed to non-specific mouse IgG treatment) of CLP mice on the ex vivo intracellular cytokine staining of isolated liver NKT cells (as delineated by CD3 + DX5 + ) (A-E) or liver macrophage/ Kupffer cells (as delineated by F4/80 + ) (F-H) , derived from Balb/c mice. Liver NKT cells exhibited a marked increase only in the frequency of cells expressing the IL-4 and IL-10 but not IFN-γ, TNF-α, IL-6 or at 24 h post-CLP that was partially blocked by anti-CD1d treatment. (A-E) . Alternatively, CLP/IgG treated mouse liver macrophages show an increase in the percentage of TNF-α + and IL-6 + cells at 24 h post-CLP. Only the increased IL-6 production was decreased by pre-treatment with anti-CD1d antibodies. (F-H) . Results are given as mean ± SEM. *, indicates significance at p
    Figure Legend Snippet: Anti-CD1d treatment leads to reduced iNKT cell cytokine production after CLP Assessment of the effect of in vivo anti-CD1d antibody pre-treatment (as opposed to non-specific mouse IgG treatment) of CLP mice on the ex vivo intracellular cytokine staining of isolated liver NKT cells (as delineated by CD3 + DX5 + ) (A-E) or liver macrophage/ Kupffer cells (as delineated by F4/80 + ) (F-H) , derived from Balb/c mice. Liver NKT cells exhibited a marked increase only in the frequency of cells expressing the IL-4 and IL-10 but not IFN-γ, TNF-α, IL-6 or at 24 h post-CLP that was partially blocked by anti-CD1d treatment. (A-E) . Alternatively, CLP/IgG treated mouse liver macrophages show an increase in the percentage of TNF-α + and IL-6 + cells at 24 h post-CLP. Only the increased IL-6 production was decreased by pre-treatment with anti-CD1d antibodies. (F-H) . Results are given as mean ± SEM. *, indicates significance at p

    Techniques Used: In Vivo, Mouse Assay, Ex Vivo, Staining, Isolation, Derivative Assay, Expressing

    6) Product Images from "An ?-galactosylceramide C20:2 N-acyl variant enhances anti-inflammatory and regulatory T cell-independent responses that prevent type 1 diabetes"

    Article Title: An ?-galactosylceramide C20:2 N-acyl variant enhances anti-inflammatory and regulatory T cell-independent responses that prevent type 1 diabetes

    Journal: Clinical and Experimental Immunology

    doi: 10.1111/j.1365-2249.2009.04074.x

    C20:2-activated invariant natural killer T (iNK T) cells induce a T helper type 2 (Th2)-biased cytokine response. (a) Structures of synthetic glycolipids α-galactosyl-ceramide (α-GalCer) C26:0 (KRN7000) and α-GalCer C20:2. Splenocytes from non-obese diabetic (NOD) mice (5–6 weeks old) were stimulated in vitro with the indicated concentrations of glycolipids for 72 h, and were then assayed by [ 3 H]-thymidine incorporation for their proliferative capacity (b) or enzyme-linked immunosorbent assay (ELISA) for their cytokine secretion (c). Data show the means ± standard deviation (s.d.) for six replicates pooled from two independent experiments containing three mice per group. (d) NOD mice (5–6 weeks old) were injected once intravenously with 500 µg of anti-CD25 monoclonal antibody or immunoglobulin G (IgG), rested for 3 days, and then treated intraperitoneally with 4 µg of KRN7000, C20:2 or vehicle. Serum was collected from the mice at the indicated times post-treatment and analysed by ELISA for the concentrations of interleukin-4 and interferon-γ. Data show the means ± s.d. of three to five individual mice per group. * P
    Figure Legend Snippet: C20:2-activated invariant natural killer T (iNK T) cells induce a T helper type 2 (Th2)-biased cytokine response. (a) Structures of synthetic glycolipids α-galactosyl-ceramide (α-GalCer) C26:0 (KRN7000) and α-GalCer C20:2. Splenocytes from non-obese diabetic (NOD) mice (5–6 weeks old) were stimulated in vitro with the indicated concentrations of glycolipids for 72 h, and were then assayed by [ 3 H]-thymidine incorporation for their proliferative capacity (b) or enzyme-linked immunosorbent assay (ELISA) for their cytokine secretion (c). Data show the means ± standard deviation (s.d.) for six replicates pooled from two independent experiments containing three mice per group. (d) NOD mice (5–6 weeks old) were injected once intravenously with 500 µg of anti-CD25 monoclonal antibody or immunoglobulin G (IgG), rested for 3 days, and then treated intraperitoneally with 4 µg of KRN7000, C20:2 or vehicle. Serum was collected from the mice at the indicated times post-treatment and analysed by ELISA for the concentrations of interleukin-4 and interferon-γ. Data show the means ± s.d. of three to five individual mice per group. * P

    Techniques Used: Mouse Assay, In Vitro, Enzyme-linked Immunosorbent Assay, Standard Deviation, Injection

    7) Product Images from "Plasmacytoid Dendritic Cells Mediate the Regulation of Inflammatory Type T Cell Response for Optimal Immunity against Respiratory Chlamydia Pneumoniae Infection"

    Article Title: Plasmacytoid Dendritic Cells Mediate the Regulation of Inflammatory Type T Cell Response for Optimal Immunity against Respiratory Chlamydia Pneumoniae Infection

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0083463

    pDC deficiency in vivo leads to altered cytokine responses. A , pDC depleted and the sham-treated mice (four mice/group), after Cpn infection were sacrificed 9 days postinfection and the lungs and draining (mediastinum) lymph nodes were collected. The draining lymph node (dLN) cells were cultured in the presence of SK-EB as described in Materials and Methods . Cytokine levels (IFN-γ, TNFα, IL-4 and IL-10) in 72-h dLN cell culture supernatants ( A ) and lung homogenates ( B ) were measured by ELISA. Data are presented as the mean ± SD of each group. Results of one of the three experiments with similar results are shown. *, p
    Figure Legend Snippet: pDC deficiency in vivo leads to altered cytokine responses. A , pDC depleted and the sham-treated mice (four mice/group), after Cpn infection were sacrificed 9 days postinfection and the lungs and draining (mediastinum) lymph nodes were collected. The draining lymph node (dLN) cells were cultured in the presence of SK-EB as described in Materials and Methods . Cytokine levels (IFN-γ, TNFα, IL-4 and IL-10) in 72-h dLN cell culture supernatants ( A ) and lung homogenates ( B ) were measured by ELISA. Data are presented as the mean ± SD of each group. Results of one of the three experiments with similar results are shown. *, p

    Techniques Used: In Vivo, Mouse Assay, Infection, Cell Culture, Enzyme-linked Immunosorbent Assay

    pDC deficiency leads to inflammatory type effector CD8 T and CD4 cell responses after Cpn infection. Lung T cells as described in the legends to Figure 4 were analyzed by multi-color intracellular cytokine staining. Shown are representative flowcytometry dot plot images and summary graphs depicting the nature of cytokine responses by T cells. Analysis was performed on gated CD3+ CD8+ cells and CD3+CD4+ cells. Note increased inflammatory type-1 (IFNγ+TNFα+) CD8 ( A ) and CD4 T cells ( B ) and inflammatory Th2 type (IL-4+ TNFα+) CD4 T cells ( C ) in the lungs of pDC depleted mice compared to the control group mice. At least three independent experiments were performed and the data from one representative experiment is depicted. Data expressed as mean ± SD. *, p
    Figure Legend Snippet: pDC deficiency leads to inflammatory type effector CD8 T and CD4 cell responses after Cpn infection. Lung T cells as described in the legends to Figure 4 were analyzed by multi-color intracellular cytokine staining. Shown are representative flowcytometry dot plot images and summary graphs depicting the nature of cytokine responses by T cells. Analysis was performed on gated CD3+ CD8+ cells and CD3+CD4+ cells. Note increased inflammatory type-1 (IFNγ+TNFα+) CD8 ( A ) and CD4 T cells ( B ) and inflammatory Th2 type (IL-4+ TNFα+) CD4 T cells ( C ) in the lungs of pDC depleted mice compared to the control group mice. At least three independent experiments were performed and the data from one representative experiment is depicted. Data expressed as mean ± SD. *, p

    Techniques Used: Infection, Staining, Mouse Assay

    T cell specific cytokine pattern in the lungs of pDC depleted mice following Cpn infection. Following Cpn infection (day 9), the cytokine pattern of CD8 and CD4 T cells in the lungs of pDC depleted and control mice was analysed by intracellular cytokine staining. Graphs show the summary data for TNFα ( A ), and IFNγ ( B ) production by CD8 and CD4 T cells and IL-4 ( C ) and IL-10 ( D ) production by CD4 T cells. Data are expressed as mean ± SD. Three independent experiments with three mice in each group were performed and one representative experiment is shown. *, p
    Figure Legend Snippet: T cell specific cytokine pattern in the lungs of pDC depleted mice following Cpn infection. Following Cpn infection (day 9), the cytokine pattern of CD8 and CD4 T cells in the lungs of pDC depleted and control mice was analysed by intracellular cytokine staining. Graphs show the summary data for TNFα ( A ), and IFNγ ( B ) production by CD8 and CD4 T cells and IL-4 ( C ) and IL-10 ( D ) production by CD4 T cells. Data are expressed as mean ± SD. Three independent experiments with three mice in each group were performed and one representative experiment is shown. *, p

    Techniques Used: Mouse Assay, Infection, Staining

    8) Product Images from "Recent thymic emigrants are biased against the T-helper type 1 and toward the T-helper type 2 effector lineage"

    Article Title: Recent thymic emigrants are biased against the T-helper type 1 and toward the T-helper type 2 effector lineage

    Journal: Blood

    doi: 10.1182/blood-2010-07-299263

    CD4 + RTEs are biased toward the Th2 effector lineage in vitro . (A) CD4 + RTEs and MN T cells were differentiated under Th2 conditions for 5 days and then stained for IL-4. Numbers in quadrants of representative flow cytometry plots show the percentage
    Figure Legend Snippet: CD4 + RTEs are biased toward the Th2 effector lineage in vitro . (A) CD4 + RTEs and MN T cells were differentiated under Th2 conditions for 5 days and then stained for IL-4. Numbers in quadrants of representative flow cytometry plots show the percentage

    Techniques Used: In Vitro, Staining, Flow Cytometry, Cytometry

    Related Articles

    Cell Culture:

    Article Title: Coxsackievirus B3 Directly Induced Th17 Cell Differentiation by Inhibiting Nup98 Expression in Patients with Acute Viral Myocarditis
    Article Snippet: This system was cultured for 2 h in 1 mL serum-free 1640 medium. .. After washing, cells were cultured with 1640 medium containing 5% FBS, 5 μg/mL of anti-CD3 (ebioscience), 2 μg/mL soluble anti-CD28 (eBioscience), 10 μg/mL anti-IL-4 (ebioscience), and 10 μg/mL anti-IFN-γ (ebioscience) for 5 days at room temperature. ..

    Article Title: Recent thymic emigrants are biased against the T-helper type 1 and toward the T-helper type 2 effector lineage
    Article Snippet: .. Cells were cultured with 30-500 ng/mL anti-CD3 (145-2C11; BD Biosciences) and 1 μg/mL anti-CD28 (37.51; eBioscience) for Th0 differentiation and were supplemented with 5 ng/mL recombinant IL-12p70 (rIL-12p70) and 10 μg/mL anti-IL-4 for Th1; 50 ng/mL rIL-4, 50 μg/mL anti-IFN-γ, and 50 μg/mL anti-IL-12/IL-23 for Th2; 10 ng/mL rIL-23, 5 ng/mL recombinant transforming growth factor-β1 (rTGF-β1), 20 ng/mL rIL-6, 10 μg/mL anti-IFN-γ, and 10 μg/mL anti-IL-4 for Th17; and 2 ng/mL rTGF-β1 for iTreg (all from eBioscience). .. For coculture experiments, purified CD45.2+ GFP+ RTEs and CD45.1+ GFP− MN T cells were mixed at various ratios and cultured with CD45.1+ CD45.2+ antigen-presenting cells as described above.

    Incubation:

    Article Title: The Role of Hepatic Invariant (I)NKT Cells in Systemic/Local Inflammation and Mortality During Polymicrobial Septic Shock
    Article Snippet: Note, we utilized anti-NK1.1 or anti-DX5 antibody as opposed to α-GalCer loaded-CD1d tetramer, on C57BL/6J or Balb/c mice respectively, as the tetramer staining was lost during the latter steps of the intracellular cytokine staining protocol. .. Cells were then permeabilized using freshly prepared Fix’Perm reagent (eBioscience, San Diego, CA) and incubated with PE-labeled anti-IL-2, anti-IL-6, anti-IL-10, anti-IL-4, anti-TNF-α, anti-MCP-1, anti-IFN-γ antibodies or the appropriate isotype controls (eBioscience, San Diego, CA). ..

    Affinity Magnetic Separation:

    Article Title: An ?-galactosylceramide C20:2 N-acyl variant enhances anti-inflammatory and regulatory T cell-independent responses that prevent type 1 diabetes
    Article Snippet: C20:2 was synthesized as described previously [ ] and dissolved at 200 µg/ml in phosphate-buffered saline (PBS) containing 0·05% Tween 20, and injected i.p. (4 µg/mouse, in 250 µl of vehicle consisting of PBS plus 0·05% Tween 20) or vehicle (PBS plus 0·05% Tween 20). .. Fluorescein isothiocyanate (FITC)-conjugated anti-TCR-β (H57-597), anti-CD25 (7D4), anti-B220 (RA3-6B2), anti-Pan NK cells (DX5), anti-Siglec H (eBio440c); phycoerythrin (PE)-conjugated anti-CD69 (H1·2F3), anti-I-Ad (AMS-32·1), anti-CD86 (Gl-1), anti-CD40 (MR1), anti-IL-4 (11B11), anti-IFN-γ (XMG1·2), anti-IL-12p40/70 (C15·6); peridinin chlorophyll (PerCP)-conjugated anti-CD8α (53–2·1), anti-CD4 (RM4-5), anti-CD3ε (145-2C11); and APC-conjugated anti-CD11c (N418) mAbs were purchased from eBiosciences (San Diego, CA, USA) or BD Biosciences (Mississauga, ON, Canada). ..

    Recombinant:

    Article Title: Recent thymic emigrants are biased against the T-helper type 1 and toward the T-helper type 2 effector lineage
    Article Snippet: .. Cells were cultured with 30-500 ng/mL anti-CD3 (145-2C11; BD Biosciences) and 1 μg/mL anti-CD28 (37.51; eBioscience) for Th0 differentiation and were supplemented with 5 ng/mL recombinant IL-12p70 (rIL-12p70) and 10 μg/mL anti-IL-4 for Th1; 50 ng/mL rIL-4, 50 μg/mL anti-IFN-γ, and 50 μg/mL anti-IL-12/IL-23 for Th2; 10 ng/mL rIL-23, 5 ng/mL recombinant transforming growth factor-β1 (rTGF-β1), 20 ng/mL rIL-6, 10 μg/mL anti-IFN-γ, and 10 μg/mL anti-IL-4 for Th17; and 2 ng/mL rTGF-β1 for iTreg (all from eBioscience). .. For coculture experiments, purified CD45.2+ GFP+ RTEs and CD45.1+ GFP− MN T cells were mixed at various ratios and cultured with CD45.1+ CD45.2+ antigen-presenting cells as described above.

    Staining:

    Article Title: Plasmacytoid Dendritic Cells Mediate the Regulation of Inflammatory Type T Cell Response for Optimal Immunity against Respiratory Chlamydia Pneumoniae Infection
    Article Snippet: Cell surface staining for T cell markers CD3, CD8 and CD4 was performed first, followed by fixation and permeabilization using IC fixation and permeabilization buffers (eBioscience) as per manufacturer’s instructions. .. The cells were further stained intracellularly for cytokines using allophycocyanin or PE labeled anti-TNF, anti-IFNγ, anti-IL-4 and anti-IL-10 (eBioscience) or with corresponding isotope control Abs and analyzed by flow cytometry. .. pDC- CD4 T Cell Co-culture The ability of pDCs to activate C. pneumoniae Ag-specific T cells and to induce Treg response was assessed using a pDC-T cell co-culture system.

    Article Title: An association of Aquaporin-4 with the immunoregulation of liver pathology in mice infected with Schistosoma japonicum
    Article Snippet: Cell staining and FCM analysis For intracellular IFN-γ ⁄ IL-4 ⁄ IL-17 staining and detection, 2 × 106 splenocytes, lymphocytes, or liver cells from schistosome-infected or normal mice were surface stained with anti-CD3-APC mAbs (eBioscience, San Diego, CA) and anti-CD4-FITC mAbs for 30 minutes. .. Cells were washed, fixed and permeabilized with Cytofix/Cytoperm buffer (BD Pharmingen) for 40 minutes and then intracellularly stained with PE-conjugated anti-IFN-γ, anti-IL-4 or anti-IL-17 respectively (eBioscience) for 60 minutes. ..

    Labeling:

    Article Title: Plasmacytoid Dendritic Cells Mediate the Regulation of Inflammatory Type T Cell Response for Optimal Immunity against Respiratory Chlamydia Pneumoniae Infection
    Article Snippet: Cell surface staining for T cell markers CD3, CD8 and CD4 was performed first, followed by fixation and permeabilization using IC fixation and permeabilization buffers (eBioscience) as per manufacturer’s instructions. .. The cells were further stained intracellularly for cytokines using allophycocyanin or PE labeled anti-TNF, anti-IFNγ, anti-IL-4 and anti-IL-10 (eBioscience) or with corresponding isotope control Abs and analyzed by flow cytometry. .. pDC- CD4 T Cell Co-culture The ability of pDCs to activate C. pneumoniae Ag-specific T cells and to induce Treg response was assessed using a pDC-T cell co-culture system.

    Flow Cytometry:

    Article Title: Plasmacytoid Dendritic Cells Mediate the Regulation of Inflammatory Type T Cell Response for Optimal Immunity against Respiratory Chlamydia Pneumoniae Infection
    Article Snippet: Cell surface staining for T cell markers CD3, CD8 and CD4 was performed first, followed by fixation and permeabilization using IC fixation and permeabilization buffers (eBioscience) as per manufacturer’s instructions. .. The cells were further stained intracellularly for cytokines using allophycocyanin or PE labeled anti-TNF, anti-IFNγ, anti-IL-4 and anti-IL-10 (eBioscience) or with corresponding isotope control Abs and analyzed by flow cytometry. .. pDC- CD4 T Cell Co-culture The ability of pDCs to activate C. pneumoniae Ag-specific T cells and to induce Treg response was assessed using a pDC-T cell co-culture system.

    Cytometry:

    Article Title: Plasmacytoid Dendritic Cells Mediate the Regulation of Inflammatory Type T Cell Response for Optimal Immunity against Respiratory Chlamydia Pneumoniae Infection
    Article Snippet: Cell surface staining for T cell markers CD3, CD8 and CD4 was performed first, followed by fixation and permeabilization using IC fixation and permeabilization buffers (eBioscience) as per manufacturer’s instructions. .. The cells were further stained intracellularly for cytokines using allophycocyanin or PE labeled anti-TNF, anti-IFNγ, anti-IL-4 and anti-IL-10 (eBioscience) or with corresponding isotope control Abs and analyzed by flow cytometry. .. pDC- CD4 T Cell Co-culture The ability of pDCs to activate C. pneumoniae Ag-specific T cells and to induce Treg response was assessed using a pDC-T cell co-culture system.

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    Thermo Fisher anti human ifn γ
    PSGR-derived peptide-induced T cell responses were CD8 + T cell dependent and restricted by HLA-I. PSGR-derived peptide-specific T cells were co-cultured with T2 cells pulsed with or without a given peptide in the presence of GolgiStop in a 48-well plate for 4 hrs at 37°C. Cells were stained with anti-CD8 and <t>anti-IFN-γ,</t> then analyzed on a FACScalibur machine (A). PSGR-derived peptide-specific T cells were co-incubated with LNCaP cells alone in medium, or with LNCaP cells in the presence of either anti-HLA-I mAb (W6/32), HLA-II mAb or a control mAb (anti-CD19 mAb). After 4 hours of incubation, the cytotoxicity against LNCaP was determined by the LDH assay (B). Data from B are plotted as means ± SD. Results are representative of three independent experiments. * P
    Anti Human Ifn γ, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human ifn γ/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
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    anti human ifn γ - by Bioz Stars, 2021-06
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    93
    Thermo Fisher biotin anti human il 4
    Elispot IFN-γ and <t>IL-4</t> responses to PvMSP9 synthetic peptides (pE, pH, pJ, pK and pL) in individuals naturally exposed to malaria infection from Colina and Ribeirinha. Frequency of IFN-γ and IL-4 responders for each peptide in individuals
    Biotin Anti Human Il 4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher anti mouse il 15
    Role of homeostatic cytokines in CTX-induced tumor rejection. DBA/2 mice were inoculated s.c. with 2 × 10 6 P815 P1.HTR tumor cells and treated i.p. with CTX (1.5 or 3 mg) or PBS 10 d later. Draining lymph nodes and tumor cells were harvested and analyzed 8 d after CTX injection. (A) Relative mRNA levels of IL-7 and <t>IL-15</t> to Ubiquitin in draining lymph nodes and tumors. Data represent the mean ± SEM from three to five experiments with 2–7 mice per group. Statistical significance was determined by the Mann–Whitney test. (B–D) Effect of IL-15 blockade on tumor growth (B, D) and survival (C, D). 10 d after tumor inoculation, mice were treated i.p. with CTX (1.5 mg) or PBS, and blocking antibodies to IL-15 or rat isotype-matched control Igs (25 µg every other day from day 10 to 16). Data are representative of (B, C) one experiment or (D) display the summary of two independent experiments with 6–9 mice per group. Statistical significance was determined by the Kruskal-Wallis (B, D) or Log-rank tests (C). * p
    Anti Mouse Il 15, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher anti cd127 antibody
    IL‐7‐IL‐7R signalling suppresses macrophage autophagy in Schistosoma japonicum ‐infected mice. A, Total RNAs of RAW 264.7, BV‐2, purified peritoneal macrophages (PMΦs) from normal mice, and FACS sorted liver macrophages from normal mice were analysed for Cd127 mRNA expression by RT‐PCR followed by agarose gel electrophoresis. B, Expression of CD127 was measured by FCM. Grey‐filled lines in FCM plots indicate the isotype control, and unfilled lines indicate the CD127‐stained cells. Agarose gel and FCM plots are representative of three independent experiments. S. japonicum ‐infected mice were injected with PBS, IL‐7, goat IgG isotype antibody, anti‐IL‐7 neutralizing antibody, rat IgG isotype antibody or <t>anti‐CD127</t> blocking antibody as described in Methods. Autophagosomes in macrophages of liver tissue were detected by TEM. C, Images were taken at either 12 000× or 40 000×. The 40 000× image is the enlarged image in the black frame. Black arrows in 40 000× images indicate double‐membraned autophagosomes. Images shown are representative of experiments. D, Data were means ± SD of 150 macrophages in 18 mice from three independent experiments. (* P ≤ .05)
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    Image Search Results


    PSGR-derived peptide-induced T cell responses were CD8 + T cell dependent and restricted by HLA-I. PSGR-derived peptide-specific T cells were co-cultured with T2 cells pulsed with or without a given peptide in the presence of GolgiStop in a 48-well plate for 4 hrs at 37°C. Cells were stained with anti-CD8 and anti-IFN-γ, then analyzed on a FACScalibur machine (A). PSGR-derived peptide-specific T cells were co-incubated with LNCaP cells alone in medium, or with LNCaP cells in the presence of either anti-HLA-I mAb (W6/32), HLA-II mAb or a control mAb (anti-CD19 mAb). After 4 hours of incubation, the cytotoxicity against LNCaP was determined by the LDH assay (B). Data from B are plotted as means ± SD. Results are representative of three independent experiments. * P

    Journal: PLoS ONE

    Article Title: Identification of Prostate-Specific G-Protein Coupled Receptor as a Tumor Antigen Recognized by CD8+ T Cells for Cancer Immunotherapy

    doi: 10.1371/journal.pone.0045756

    Figure Lengend Snippet: PSGR-derived peptide-induced T cell responses were CD8 + T cell dependent and restricted by HLA-I. PSGR-derived peptide-specific T cells were co-cultured with T2 cells pulsed with or without a given peptide in the presence of GolgiStop in a 48-well plate for 4 hrs at 37°C. Cells were stained with anti-CD8 and anti-IFN-γ, then analyzed on a FACScalibur machine (A). PSGR-derived peptide-specific T cells were co-incubated with LNCaP cells alone in medium, or with LNCaP cells in the presence of either anti-HLA-I mAb (W6/32), HLA-II mAb or a control mAb (anti-CD19 mAb). After 4 hours of incubation, the cytotoxicity against LNCaP was determined by the LDH assay (B). Data from B are plotted as means ± SD. Results are representative of three independent experiments. * P

    Article Snippet: Briefly, 96-well ELISPOT plates (Millipore; Bedford, MA, USA) were coated overnight at 4°C with 7.5 µg/mL anti-human IFN-γ (Pierce Biotechnology; Rockford, IL, USA).

    Techniques: Derivative Assay, Cell Culture, Staining, Incubation, Lactate Dehydrogenase Assay

    PSGR-derived peptides induced peptide-specific T cells. The recognition of T2 cells pre-loaded with titrated concentrations of peptides (0–20 µg/ml) by expanded PSGR peptide-specific T cells was tested by ELISA assay (A). The expanded PSGR3 T cells (B and E), PSGR4 T cells (C and F) and PSGR14 T cells (D and G) were respectively co-incubated with T2 cells (1×10 4 cells/well) alone in complete medium (CM), or with T2 cells pre-loaded with either a corresponding peptide (5 µg/mL) or a control peptide as a negative control. Cells were incubated for 18 −24 hours, the IFN-γ secretion in the supernatant was determined by ELISA assay (B, C and D). IFN-γ spot-forming cells (SFC) were enumerated by ELISPOT assay (E, F and G). Data are plotted as means ± SD. Results are representative of at least three independent experiments. * P

    Journal: PLoS ONE

    Article Title: Identification of Prostate-Specific G-Protein Coupled Receptor as a Tumor Antigen Recognized by CD8+ T Cells for Cancer Immunotherapy

    doi: 10.1371/journal.pone.0045756

    Figure Lengend Snippet: PSGR-derived peptides induced peptide-specific T cells. The recognition of T2 cells pre-loaded with titrated concentrations of peptides (0–20 µg/ml) by expanded PSGR peptide-specific T cells was tested by ELISA assay (A). The expanded PSGR3 T cells (B and E), PSGR4 T cells (C and F) and PSGR14 T cells (D and G) were respectively co-incubated with T2 cells (1×10 4 cells/well) alone in complete medium (CM), or with T2 cells pre-loaded with either a corresponding peptide (5 µg/mL) or a control peptide as a negative control. Cells were incubated for 18 −24 hours, the IFN-γ secretion in the supernatant was determined by ELISA assay (B, C and D). IFN-γ spot-forming cells (SFC) were enumerated by ELISPOT assay (E, F and G). Data are plotted as means ± SD. Results are representative of at least three independent experiments. * P

    Article Snippet: Briefly, 96-well ELISPOT plates (Millipore; Bedford, MA, USA) were coated overnight at 4°C with 7.5 µg/mL anti-human IFN-γ (Pierce Biotechnology; Rockford, IL, USA).

    Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Incubation, Negative Control, Enzyme-linked Immunospot

    Elispot IFN-γ and IL-4 responses to PvMSP9 synthetic peptides (pE, pH, pJ, pK and pL) in individuals naturally exposed to malaria infection from Colina and Ribeirinha. Frequency of IFN-γ and IL-4 responders for each peptide in individuals

    Journal:

    Article Title: Naturally acquired humoral and cellular immune responses to Plasmodium vivax merozoite surface protein 9 in Northwestern Amazon individuals

    doi: 10.1016/j.vaccine.2008.09.029

    Figure Lengend Snippet: Elispot IFN-γ and IL-4 responses to PvMSP9 synthetic peptides (pE, pH, pJ, pK and pL) in individuals naturally exposed to malaria infection from Colina and Ribeirinha. Frequency of IFN-γ and IL-4 responders for each peptide in individuals

    Article Snippet: After stimulation, plates were washed four times with PBS containing 0.05% Tween 20 (PBS-T) and incubated with either biotin-anti-human IFN-γ Clone 7-B6-1 (MabTech) diluted in PBS or biotin-anti-human IL-4 Clone 12-1 NON0059 (Biosource International, Camarilla, CA) diluted in PBS-T containing 1% fetal bovine serum (PBS-TF) for 3 h at 37°C.

    Techniques: Enzyme-linked Immunospot, Infection

    Role of homeostatic cytokines in CTX-induced tumor rejection. DBA/2 mice were inoculated s.c. with 2 × 10 6 P815 P1.HTR tumor cells and treated i.p. with CTX (1.5 or 3 mg) or PBS 10 d later. Draining lymph nodes and tumor cells were harvested and analyzed 8 d after CTX injection. (A) Relative mRNA levels of IL-7 and IL-15 to Ubiquitin in draining lymph nodes and tumors. Data represent the mean ± SEM from three to five experiments with 2–7 mice per group. Statistical significance was determined by the Mann–Whitney test. (B–D) Effect of IL-15 blockade on tumor growth (B, D) and survival (C, D). 10 d after tumor inoculation, mice were treated i.p. with CTX (1.5 mg) or PBS, and blocking antibodies to IL-15 or rat isotype-matched control Igs (25 µg every other day from day 10 to 16). Data are representative of (B, C) one experiment or (D) display the summary of two independent experiments with 6–9 mice per group. Statistical significance was determined by the Kruskal-Wallis (B, D) or Log-rank tests (C). * p

    Journal: Oncoimmunology

    Article Title: Cyclophosphamide treatment regulates the balance of functional/exhausted tumor-specific CD8+ T cells

    doi: 10.1080/2162402X.2017.1318234

    Figure Lengend Snippet: Role of homeostatic cytokines in CTX-induced tumor rejection. DBA/2 mice were inoculated s.c. with 2 × 10 6 P815 P1.HTR tumor cells and treated i.p. with CTX (1.5 or 3 mg) or PBS 10 d later. Draining lymph nodes and tumor cells were harvested and analyzed 8 d after CTX injection. (A) Relative mRNA levels of IL-7 and IL-15 to Ubiquitin in draining lymph nodes and tumors. Data represent the mean ± SEM from three to five experiments with 2–7 mice per group. Statistical significance was determined by the Mann–Whitney test. (B–D) Effect of IL-15 blockade on tumor growth (B, D) and survival (C, D). 10 d after tumor inoculation, mice were treated i.p. with CTX (1.5 mg) or PBS, and blocking antibodies to IL-15 or rat isotype-matched control Igs (25 µg every other day from day 10 to 16). Data are representative of (B, C) one experiment or (D) display the summary of two independent experiments with 6–9 mice per group. Statistical significance was determined by the Kruskal-Wallis (B, D) or Log-rank tests (C). * p

    Article Snippet: When indicated, mice were injected intraperitoneally (i.p.) with cyclophosphamide (CTX) monohydrate (Sigma-Aldrich, 150 mg/kg, at day 10 after tumor inoculation) and/or with : anti-mouse IFNAR-1 (clone MAR1-5A3, BioXCell, 500 µg at day 10 after tumor inoculation, followed by 250 µg at days 12, 14 and 17), anti-mouse IL-7Rα (clone A7R34, BioXCell, 450 µg every other day from day 10 to day 16 after tumor inoculation), anti-mouse IL-15 (clone AIO.3, eBioscience, 25 µg every day from day 10 to day 16 after tumor inoculation).

    Techniques: Mouse Assay, Injection, MANN-WHITNEY, Blocking Assay

    IL‐7‐IL‐7R signalling suppresses macrophage autophagy in Schistosoma japonicum ‐infected mice. A, Total RNAs of RAW 264.7, BV‐2, purified peritoneal macrophages (PMΦs) from normal mice, and FACS sorted liver macrophages from normal mice were analysed for Cd127 mRNA expression by RT‐PCR followed by agarose gel electrophoresis. B, Expression of CD127 was measured by FCM. Grey‐filled lines in FCM plots indicate the isotype control, and unfilled lines indicate the CD127‐stained cells. Agarose gel and FCM plots are representative of three independent experiments. S. japonicum ‐infected mice were injected with PBS, IL‐7, goat IgG isotype antibody, anti‐IL‐7 neutralizing antibody, rat IgG isotype antibody or anti‐CD127 blocking antibody as described in Methods. Autophagosomes in macrophages of liver tissue were detected by TEM. C, Images were taken at either 12 000× or 40 000×. The 40 000× image is the enlarged image in the black frame. Black arrows in 40 000× images indicate double‐membraned autophagosomes. Images shown are representative of experiments. D, Data were means ± SD of 150 macrophages in 18 mice from three independent experiments. (* P ≤ .05)

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: IL‐7 suppresses macrophage autophagy and promotes liver pathology in Schistosoma japonicum‐infected mice, et al. IL‐7 suppresses macrophage autophagy and promotes liver pathology in Schistosoma japonicum‐infected mice

    doi: 10.1111/jcmm.13610

    Figure Lengend Snippet: IL‐7‐IL‐7R signalling suppresses macrophage autophagy in Schistosoma japonicum ‐infected mice. A, Total RNAs of RAW 264.7, BV‐2, purified peritoneal macrophages (PMΦs) from normal mice, and FACS sorted liver macrophages from normal mice were analysed for Cd127 mRNA expression by RT‐PCR followed by agarose gel electrophoresis. B, Expression of CD127 was measured by FCM. Grey‐filled lines in FCM plots indicate the isotype control, and unfilled lines indicate the CD127‐stained cells. Agarose gel and FCM plots are representative of three independent experiments. S. japonicum ‐infected mice were injected with PBS, IL‐7, goat IgG isotype antibody, anti‐IL‐7 neutralizing antibody, rat IgG isotype antibody or anti‐CD127 blocking antibody as described in Methods. Autophagosomes in macrophages of liver tissue were detected by TEM. C, Images were taken at either 12 000× or 40 000×. The 40 000× image is the enlarged image in the black frame. Black arrows in 40 000× images indicate double‐membraned autophagosomes. Images shown are representative of experiments. D, Data were means ± SD of 150 macrophages in 18 mice from three independent experiments. (* P ≤ .05)

    Article Snippet: 2.6 FCM analysis For labelling of CD127, cells were stained for 30 minutes at 4°C with anti‐CD127 antibody (eBioscience, San Diego, CA) diluted in PBS containing 1% FBS.

    Techniques: Infection, Mouse Assay, Purification, FACS, Expressing, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining, Injection, Blocking Assay, Transmission Electron Microscopy