anti igg2b  (SouthernBiotech)

 
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  • 93
    Name:
    Rabbit F ab 2 Anti Goat IgG H L Human SP ads UNLB
    Description:

    Catalog Number:
    6026-01
    Price:
    None
    Source:
    Pepsin digest of Rabbit Anti-Goat IgG(H+L), Human SP ads (SB Cat. No. 6164)
    Applications:
    Quality tested applications for relevant formats include -ELISAFLISAOther referenced applications for relevant formats include -Flow Cytometry 1Western Blot 2
    Format:
    UNLB (Unconjugated)
    Isotype:
    Rabbit F(ab')2 IgG
    Buy from Supplier


    Structured Review

    SouthernBiotech anti igg2b
    Rabbit F ab 2 Anti Goat IgG H L Human SP ads UNLB

    https://www.bioz.com/result/anti igg2b/product/SouthernBiotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti igg2b - by Bioz Stars, 2021-05
    93/100 stars

    Images

    1) Product Images from "Reduction of CD1d expression in vivo minimally affects NKT-enhanced antibody production but boosts B-cell memory"

    Article Title: Reduction of CD1d expression in vivo minimally affects NKT-enhanced antibody production but boosts B-cell memory

    Journal: International Immunology

    doi: 10.1093/intimm/dxq477

    Comparison of transferable B-cell memory in immunized CD1d +/+ and CD1d +/− mice. CD1d +/+ and CD1d +/− mice were immunized with NP-KLH plus α-GC and after 60 days, splenocytes were harvested. (A) Total splenocytes were adoptively transferred to CD45.1 C57Bl/6 recipients before administration of NP-KLH and collection of sera after 7 and 21 days. ELISAS were performed to determine end-point anti-NP IgG1 titers. (B) B cells were adoptively transferred to IgH a C57Bl/6 congenic mice before administration of NP-KLH and collection of sera on day 21. ELISAS were performed to determine NP-specific IgH b antibody responses. NP-specific IgH a was not detected (not depicted). Data show (A) mean ± SEM end-point titer or (B) absorbance at a one in 200 dilution of sera for three mice per group.
    Figure Legend Snippet: Comparison of transferable B-cell memory in immunized CD1d +/+ and CD1d +/− mice. CD1d +/+ and CD1d +/− mice were immunized with NP-KLH plus α-GC and after 60 days, splenocytes were harvested. (A) Total splenocytes were adoptively transferred to CD45.1 C57Bl/6 recipients before administration of NP-KLH and collection of sera after 7 and 21 days. ELISAS were performed to determine end-point anti-NP IgG1 titers. (B) B cells were adoptively transferred to IgH a C57Bl/6 congenic mice before administration of NP-KLH and collection of sera on day 21. ELISAS were performed to determine NP-specific IgH b antibody responses. NP-specific IgH a was not detected (not depicted). Data show (A) mean ± SEM end-point titer or (B) absorbance at a one in 200 dilution of sera for three mice per group.

    Techniques Used: Mouse Assay

    Similar antibody titers in CD1d +/+ and CD1d +/− mice. CD1d +/+ and CD1d +/− C57Bl/6 mice were bled and then immunized s.c. with 10 μg NP-KLH or NP-KLH plus 4 μg α-GC. After 28 days, mice were bled (A, primary) and given a booster vaccine (10 μg NP-KLH s.c.) before collecting sera on day 35 (B, secondary) and day 140 (C, tertiary). Each data point represents the end-point anti-NP IgG1 titer for a single mouse. Data are representative of three independent experiments.
    Figure Legend Snippet: Similar antibody titers in CD1d +/+ and CD1d +/− mice. CD1d +/+ and CD1d +/− C57Bl/6 mice were bled and then immunized s.c. with 10 μg NP-KLH or NP-KLH plus 4 μg α-GC. After 28 days, mice were bled (A, primary) and given a booster vaccine (10 μg NP-KLH s.c.) before collecting sera on day 35 (B, secondary) and day 140 (C, tertiary). Each data point represents the end-point anti-NP IgG1 titer for a single mouse. Data are representative of three independent experiments.

    Techniques Used: Mouse Assay

    2) Product Images from "Modulation of adaptive immunity by different adjuvant-antigen combinations in mice lacking Nod2"

    Article Title: Modulation of adaptive immunity by different adjuvant-antigen combinations in mice lacking Nod2

    Journal: Vaccine

    doi: 10.1016/j.vaccine.2008.08.038

    Immunization of control ( Myd88 +/+ ) or MyD88-deficient mice immunized with TB-OTII-NE236 peptide plus IFA. Mice were immunized as described in . Shown are IgG1 antibody titers specific for NE236. Antigen-specific IgM, IgG2b and IgG2c responses were
    Figure Legend Snippet: Immunization of control ( Myd88 +/+ ) or MyD88-deficient mice immunized with TB-OTII-NE236 peptide plus IFA. Mice were immunized as described in . Shown are IgG1 antibody titers specific for NE236. Antigen-specific IgM, IgG2b and IgG2c responses were

    Techniques Used: Mouse Assay, Immunofluorescence

    3) Product Images from "Expression of an anti-RNA autoantibody in a mouse model of SLE is sufficient to increase neutrophil and monocyte numbers as well as the amount of IFN-I"

    Article Title: Expression of an anti-RNA autoantibody in a mouse model of SLE is sufficient to increase neutrophil and monocyte numbers as well as the amount of IFN-I

    Journal: European journal of immunology

    doi: 10.1002/eji.201343714

    Increased frequency of class-switched IgG2a + BM developing B in 564Igi mice. Representative compensation single-stained controls of flow cytometry for 564 Id and IgG2a. Viable (PI - ) lymphocytes from 564Igi-B6 BM were gated to show (A) unstained or linear staining profile for (B) 564 Id or (C) IgG2a. A representative profile of AA4.1 + Id + IgG2a + cells (D) and the frequency of AA4.1 + Id + IgG2a + cells (E) from age-matched (3 m.o.) wild-type B6. Igh a and 564Igi-B6 mice. Viable BM AA4.1 + cells were gated to demonstrate the frequency of Id + IgG2a + cells. Compiled results of two independent experiments are displayed in (E). Calculations for statistical differences between various groups were carried out with a Student’s t- test. **, p
    Figure Legend Snippet: Increased frequency of class-switched IgG2a + BM developing B in 564Igi mice. Representative compensation single-stained controls of flow cytometry for 564 Id and IgG2a. Viable (PI - ) lymphocytes from 564Igi-B6 BM were gated to show (A) unstained or linear staining profile for (B) 564 Id or (C) IgG2a. A representative profile of AA4.1 + Id + IgG2a + cells (D) and the frequency of AA4.1 + Id + IgG2a + cells (E) from age-matched (3 m.o.) wild-type B6. Igh a and 564Igi-B6 mice. Viable BM AA4.1 + cells were gated to demonstrate the frequency of Id + IgG2a + cells. Compiled results of two independent experiments are displayed in (E). Calculations for statistical differences between various groups were carried out with a Student’s t- test. **, p

    Techniques Used: Mouse Assay, Staining, Flow Cytometry, Cytometry

    IFN-I dependent enhancement of autoantibody production, expression of Tlr7 and Aicda and up-regulation of IFN-I signature genes in immature B cells of 564Igi mice (A) Decrease in Id + IgG2a/IgG2b ASCs in the BMs of 564Igi-B6. Ifnar1 −/− mice. (B, C) Decrease of Tlr7 and Aicda expression in Id + autoreactive RNA-specific developing B cells (B220 + AA4.1 + Id + ) of 564Igi-B6. Ifnar1 −/− mice. Representative of 3 experiments showing relative Aicda and Tlr7 gene expression determined by qPCR with cDNA from BM ex vivo sorted immature B cells (B220 + AA4.1 + Id + ) from B6, 564Igi-B6. Tlr7 −/− , 564Igi-B6. Ifnar1 −/− and 564Igi-B6 mice. **, p
    Figure Legend Snippet: IFN-I dependent enhancement of autoantibody production, expression of Tlr7 and Aicda and up-regulation of IFN-I signature genes in immature B cells of 564Igi mice (A) Decrease in Id + IgG2a/IgG2b ASCs in the BMs of 564Igi-B6. Ifnar1 −/− mice. (B, C) Decrease of Tlr7 and Aicda expression in Id + autoreactive RNA-specific developing B cells (B220 + AA4.1 + Id + ) of 564Igi-B6. Ifnar1 −/− mice. Representative of 3 experiments showing relative Aicda and Tlr7 gene expression determined by qPCR with cDNA from BM ex vivo sorted immature B cells (B220 + AA4.1 + Id + ) from B6, 564Igi-B6. Tlr7 −/− , 564Igi-B6. Ifnar1 −/− and 564Igi-B6 mice. **, p

    Techniques Used: Expressing, Mouse Assay, Real-time Polymerase Chain Reaction, Ex Vivo

    4) Product Images from "Helicobacter bilis Infection Accelerates and H. hepaticus Infection Delays the Development of Colitis in Multiple Drug Resistance-Deficient (mdr1a−/−) Mice"

    Article Title: Helicobacter bilis Infection Accelerates and H. hepaticus Infection Delays the Development of Colitis in Multiple Drug Resistance-Deficient (mdr1a−/−) Mice

    Journal: The American Journal of Pathology

    doi:

    Serum immunoglobulin levels from Helicobacter -infected mdr1a−/− and FVB mice. Immunoglobulin isotypes (IgA, total IgG, IgG1, IgG2a) for H. bilis -infected mice (58 days post-infection) are shown in the left four panels and H. hepaticus -infected mice (17 weeks post-infection) are shown in the right four panels. H. bilis exposed mdr1a−/− animals produce a broad immunoglobulin response, while H. hepaticus -exposed mdr1a−/− animals express a more restricted repertoire. Numbers reflect means and standard deviations comprising triplicate wells of pooled sera from 2 to 3 mice in each group.
    Figure Legend Snippet: Serum immunoglobulin levels from Helicobacter -infected mdr1a−/− and FVB mice. Immunoglobulin isotypes (IgA, total IgG, IgG1, IgG2a) for H. bilis -infected mice (58 days post-infection) are shown in the left four panels and H. hepaticus -infected mice (17 weeks post-infection) are shown in the right four panels. H. bilis exposed mdr1a−/− animals produce a broad immunoglobulin response, while H. hepaticus -exposed mdr1a−/− animals express a more restricted repertoire. Numbers reflect means and standard deviations comprising triplicate wells of pooled sera from 2 to 3 mice in each group.

    Techniques Used: Infection, Mouse Assay

    5) Product Images from "Overexpression of TLR7 promotes cell-intrinsic expansion and autoantibody production by transitional T1 B cells"

    Article Title: Overexpression of TLR7 promotes cell-intrinsic expansion and autoantibody production by transitional T1 B cells

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20122798

    Anti-RNA–specific B cells in H564i Tg mice display an immature phenotype and produce class-switched IgG Abs. (A–D) Splenocytes from 6-mo-old WT and H564Igi +/− mice were analyzed by flow cytometry to assess the frequencies and phenotype of H564Id + B cells. (A) Representative flow plots show staining with anti-H564 Id Ab, used to define Id + cells (right). The middle shows the frequencies of T1, T2, FO, and MZ B cell subsets, as determined by anti-CD24 and anti-CD21 staining. The right shows overlap between Id − and Id + cells (shown in blue). (B) Summarized data from individual WT and H564Igi +/− mice showing the frequencies of CD24 hi CD21 lo cells of gated B220 + cells. (C) Data show the distribution of Id + cells within individual B cell populations in H564Igi +/− mice. (D) Histograms show the expression of CD93, CD23, IgM, and IgD on gated Id + (blue) and Id − (black) B cells in H564Igi +/− mice. (A–D) Data presented are from four independent experiments. In B and C, each dot represents an individual animal with the mean indicated for each group (horizontal bars). (E) Splenic sections were prepared from frozen spleens of WT and H564Igi +/− mice and stained with B220 (red), CD169 (blue), and H564Id (green). Data are representative of more than six sections analyzed from two mice per genotype. Bars, 100 µm. (F) Serum levels of Id IgG2a and IgG2b in individual WT and H564Igi +/− mice (6–12 mo old) were determined by ELISA. Sera dilutions: 1:15,000 (IgG2a) and 1:3,000 (IgG2b). Each dot represents an individual animal with the mean indicated for each group (horizontal bars). (G and H) Purified Id + CD93 hi and Id + CD93 lo cells from H564Igi +/− mice were cultured for 72 h in RPMI medium or stimulated with 50 ng/ml R848, and the production of antibodies in culture supernatants was measured by ELISA. (G) Gating strategy used for purification of Id + CD93 hi and Id + CD93 lo cells. (H) Bar graphs show the mean titers of IgM, IgG1, IgG2a, and IgG2b in culture supernatants produced by Id + CD93 hi and Id + CD93 lo cells. Data are representative of two independent experiments using four individual mice and presented as mean ± SD. Error bars indicate variation between the individual mice. **, P
    Figure Legend Snippet: Anti-RNA–specific B cells in H564i Tg mice display an immature phenotype and produce class-switched IgG Abs. (A–D) Splenocytes from 6-mo-old WT and H564Igi +/− mice were analyzed by flow cytometry to assess the frequencies and phenotype of H564Id + B cells. (A) Representative flow plots show staining with anti-H564 Id Ab, used to define Id + cells (right). The middle shows the frequencies of T1, T2, FO, and MZ B cell subsets, as determined by anti-CD24 and anti-CD21 staining. The right shows overlap between Id − and Id + cells (shown in blue). (B) Summarized data from individual WT and H564Igi +/− mice showing the frequencies of CD24 hi CD21 lo cells of gated B220 + cells. (C) Data show the distribution of Id + cells within individual B cell populations in H564Igi +/− mice. (D) Histograms show the expression of CD93, CD23, IgM, and IgD on gated Id + (blue) and Id − (black) B cells in H564Igi +/− mice. (A–D) Data presented are from four independent experiments. In B and C, each dot represents an individual animal with the mean indicated for each group (horizontal bars). (E) Splenic sections were prepared from frozen spleens of WT and H564Igi +/− mice and stained with B220 (red), CD169 (blue), and H564Id (green). Data are representative of more than six sections analyzed from two mice per genotype. Bars, 100 µm. (F) Serum levels of Id IgG2a and IgG2b in individual WT and H564Igi +/− mice (6–12 mo old) were determined by ELISA. Sera dilutions: 1:15,000 (IgG2a) and 1:3,000 (IgG2b). Each dot represents an individual animal with the mean indicated for each group (horizontal bars). (G and H) Purified Id + CD93 hi and Id + CD93 lo cells from H564Igi +/− mice were cultured for 72 h in RPMI medium or stimulated with 50 ng/ml R848, and the production of antibodies in culture supernatants was measured by ELISA. (G) Gating strategy used for purification of Id + CD93 hi and Id + CD93 lo cells. (H) Bar graphs show the mean titers of IgM, IgG1, IgG2a, and IgG2b in culture supernatants produced by Id + CD93 hi and Id + CD93 lo cells. Data are representative of two independent experiments using four individual mice and presented as mean ± SD. Error bars indicate variation between the individual mice. **, P

    Techniques Used: Mouse Assay, Flow Cytometry, Cytometry, Staining, Expressing, Enzyme-linked Immunosorbent Assay, Purification, Cell Culture, Produced

    TLR7.1Tg T1 B cells produce class-switched IgG. (A and B) Sorted T1 and FO B cells from WT and TLR7.1Tg mice were cultured for 72 h in RPMI medium or stimulated with anti-IgM (10 µg/ml), R848 (50 ng/ml), or a combination of anti-IgM plus R848 or SmRNP (10 ng/ml). Ab production in cell culture supernatants were analyzed by ELISA. (A) Bar graphs show quantities of total IgM and IgG produced by T1 and FO B cells in response to different stimuli. (B) Bar graphs show quantities of IgG1, IgG2b, and IgG2c produced by T1 B cells in response to different stimuli. The data are presented as mean ± SD from three independent experiments. (C) Splenic sections were prepared from frozen spleens from 5-mo-old WT and TLR7.1Tg mice and stained with anti-B220 and anti-IgG2b and anti-IgG2c Abs. Data show presence of IgG2b + and IgG2c + cells in the splenic RP of TLR7.1Tg mice and are representative of more than four sections analyzed from two mice per genotype. Bars, 100 µm. (D) Sorted T1 B cells from WT and TLR7.1Tg mice were cultured for 72 h in RPMI medium or stimulated with anti-IgM (10 µg/ml), R848 (50 ng/ml), or a combination of anti-IgM plus R848 or SmRNP (10 ng/ml). Anti-RNA IgG titers in cell culture supernatants were analyzed by ELISA. Individual dots represent data from two independent experiments. (E) Representative Hep-2 ANA staining of undiluted culture supernatants from WT or TLR7Tg T1 cells cultured for 72 h in RPMI medium or stimulated with R848, or a combination of anti-IgM plus R848. Data are representative of three independent experiments. Bars, 50 µm. (F and G) T1, T2, FO, and MZ cell subsets from WT and TLR7.1Tg mice were isolated by cell sorting and the expression of Aicda (F) and Tbx21 (G) mRNA were quantified by RT-PCR. Data are presented as fold change of mRNA levels relative to WT T1 cells. Data are average of four mice per genotype (8–12 wk old) and show mean ± SEM. *, P
    Figure Legend Snippet: TLR7.1Tg T1 B cells produce class-switched IgG. (A and B) Sorted T1 and FO B cells from WT and TLR7.1Tg mice were cultured for 72 h in RPMI medium or stimulated with anti-IgM (10 µg/ml), R848 (50 ng/ml), or a combination of anti-IgM plus R848 or SmRNP (10 ng/ml). Ab production in cell culture supernatants were analyzed by ELISA. (A) Bar graphs show quantities of total IgM and IgG produced by T1 and FO B cells in response to different stimuli. (B) Bar graphs show quantities of IgG1, IgG2b, and IgG2c produced by T1 B cells in response to different stimuli. The data are presented as mean ± SD from three independent experiments. (C) Splenic sections were prepared from frozen spleens from 5-mo-old WT and TLR7.1Tg mice and stained with anti-B220 and anti-IgG2b and anti-IgG2c Abs. Data show presence of IgG2b + and IgG2c + cells in the splenic RP of TLR7.1Tg mice and are representative of more than four sections analyzed from two mice per genotype. Bars, 100 µm. (D) Sorted T1 B cells from WT and TLR7.1Tg mice were cultured for 72 h in RPMI medium or stimulated with anti-IgM (10 µg/ml), R848 (50 ng/ml), or a combination of anti-IgM plus R848 or SmRNP (10 ng/ml). Anti-RNA IgG titers in cell culture supernatants were analyzed by ELISA. Individual dots represent data from two independent experiments. (E) Representative Hep-2 ANA staining of undiluted culture supernatants from WT or TLR7Tg T1 cells cultured for 72 h in RPMI medium or stimulated with R848, or a combination of anti-IgM plus R848. Data are representative of three independent experiments. Bars, 50 µm. (F and G) T1, T2, FO, and MZ cell subsets from WT and TLR7.1Tg mice were isolated by cell sorting and the expression of Aicda (F) and Tbx21 (G) mRNA were quantified by RT-PCR. Data are presented as fold change of mRNA levels relative to WT T1 cells. Data are average of four mice per genotype (8–12 wk old) and show mean ± SEM. *, P

    Techniques Used: Mouse Assay, Cell Culture, Enzyme-linked Immunosorbent Assay, Produced, Staining, Isolation, FACS, Expressing, Reverse Transcription Polymerase Chain Reaction

    6) Product Images from "Expression of an anti-RNA autoantibody in a mouse model of SLE is sufficient to increase neutrophil and monocyte numbers as well as the amount of IFN-I"

    Article Title: Expression of an anti-RNA autoantibody in a mouse model of SLE is sufficient to increase neutrophil and monocyte numbers as well as the amount of IFN-I

    Journal: European journal of immunology

    doi: 10.1002/eji.201343714

    Increased frequency of class-switched IgG2a + BM developing B in 564Igi mice. Representative compensation single-stained controls of flow cytometry for 564 Id and IgG2a. Viable (PI - ) lymphocytes from 564Igi-B6 BM were gated to show (A) unstained or linear staining profile for (B) 564 Id or (C) IgG2a. A representative profile of AA4.1 + Id + IgG2a + cells (D) and the frequency of AA4.1 + Id + IgG2a + cells (E) from age-matched (3 m.o.) wild-type B6. Igh a and 564Igi-B6 mice. Viable BM AA4.1 + cells were gated to demonstrate the frequency of Id + IgG2a + cells. Compiled results of two independent experiments are displayed in (E). Calculations for statistical differences between various groups were carried out with a Student’s t- test. **, p
    Figure Legend Snippet: Increased frequency of class-switched IgG2a + BM developing B in 564Igi mice. Representative compensation single-stained controls of flow cytometry for 564 Id and IgG2a. Viable (PI - ) lymphocytes from 564Igi-B6 BM were gated to show (A) unstained or linear staining profile for (B) 564 Id or (C) IgG2a. A representative profile of AA4.1 + Id + IgG2a + cells (D) and the frequency of AA4.1 + Id + IgG2a + cells (E) from age-matched (3 m.o.) wild-type B6. Igh a and 564Igi-B6 mice. Viable BM AA4.1 + cells were gated to demonstrate the frequency of Id + IgG2a + cells. Compiled results of two independent experiments are displayed in (E). Calculations for statistical differences between various groups were carried out with a Student’s t- test. **, p

    Techniques Used: Mouse Assay, Staining, Flow Cytometry, Cytometry

    IFN-I dependent enhancement of autoantibody production, expression of Tlr7 and Aicda and up-regulation of IFN-I signature genes in immature B cells of 564Igi mice (A) Decrease in Id + IgG2a/IgG2b ASCs in the BMs of 564Igi-B6. Ifnar1 −/− mice. (B, C) Decrease of Tlr7 and Aicda expression in Id + autoreactive RNA-specific developing B cells (B220 + AA4.1 + Id + ) of 564Igi-B6. Ifnar1 −/− mice. Representative of 3 experiments showing relative Aicda and Tlr7 gene expression determined by qPCR with cDNA from BM ex vivo sorted immature B cells (B220 + AA4.1 + Id + ) from B6, 564Igi-B6. Tlr7 −/− , 564Igi-B6. Ifnar1 −/− and 564Igi-B6 mice. **, p
    Figure Legend Snippet: IFN-I dependent enhancement of autoantibody production, expression of Tlr7 and Aicda and up-regulation of IFN-I signature genes in immature B cells of 564Igi mice (A) Decrease in Id + IgG2a/IgG2b ASCs in the BMs of 564Igi-B6. Ifnar1 −/− mice. (B, C) Decrease of Tlr7 and Aicda expression in Id + autoreactive RNA-specific developing B cells (B220 + AA4.1 + Id + ) of 564Igi-B6. Ifnar1 −/− mice. Representative of 3 experiments showing relative Aicda and Tlr7 gene expression determined by qPCR with cDNA from BM ex vivo sorted immature B cells (B220 + AA4.1 + Id + ) from B6, 564Igi-B6. Tlr7 −/− , 564Igi-B6. Ifnar1 −/− and 564Igi-B6 mice. **, p

    Techniques Used: Expressing, Mouse Assay, Real-time Polymerase Chain Reaction, Ex Vivo

    7) Product Images from "The Small Rho GTPase TC10 Modulates B Cell Immune Responses"

    Article Title: The Small Rho GTPase TC10 Modulates B Cell Immune Responses

    Journal: The Journal of Immunology Author Choice

    doi: 10.4049/jimmunol.1602167

    Impaired B cell responses in TC10 KO animals. ( A ) Serum was collected from naive WT and TC10 KO animals, and baseline titers of IgM, IgG2b, IgG2c, IgG3, and IgA were measured by sandwich ELISA. ( B – E ) WT and TC10 KO animals were infected with 10 4 PFU VACV (B and C) or 2.10 2 Influenza virus (D and E) by intra footpad (B and C) or intranasal injection (D and E), and the draining popliteal (B and C) or mediastinal (D and E) LNs were isolated 7 (B and C) or 9 (D and E) d later. Germinal center B cells (CD95 + GL-7 + (B and D) and plasma cells [CD138 + IgD lo (C and E)] were analyzed by flow cytometry. Quantifications are shown on the panels on the right and show the percentage of B cells in the indicated gates. ( F and G ) WT and TC10 KO mice were immunized with NP-KLH precipitated in Alum, and serum samples collected weekly for 28 d. NP-specific IgM and IgG3 (F) titers were measured, as well as total IgG titers (G). ( H ) ELISA analysis showing affinity maturation (expressed as the ratio of NP3 to NP23 titers) of total IgG in WT and TC10 KO animals. Data are from one representative of two to three independent experiments with at least three animals in each group. Student t test (ns: p > 0.05, * p
    Figure Legend Snippet: Impaired B cell responses in TC10 KO animals. ( A ) Serum was collected from naive WT and TC10 KO animals, and baseline titers of IgM, IgG2b, IgG2c, IgG3, and IgA were measured by sandwich ELISA. ( B – E ) WT and TC10 KO animals were infected with 10 4 PFU VACV (B and C) or 2.10 2 Influenza virus (D and E) by intra footpad (B and C) or intranasal injection (D and E), and the draining popliteal (B and C) or mediastinal (D and E) LNs were isolated 7 (B and C) or 9 (D and E) d later. Germinal center B cells (CD95 + GL-7 + (B and D) and plasma cells [CD138 + IgD lo (C and E)] were analyzed by flow cytometry. Quantifications are shown on the panels on the right and show the percentage of B cells in the indicated gates. ( F and G ) WT and TC10 KO mice were immunized with NP-KLH precipitated in Alum, and serum samples collected weekly for 28 d. NP-specific IgM and IgG3 (F) titers were measured, as well as total IgG titers (G). ( H ) ELISA analysis showing affinity maturation (expressed as the ratio of NP3 to NP23 titers) of total IgG in WT and TC10 KO animals. Data are from one representative of two to three independent experiments with at least three animals in each group. Student t test (ns: p > 0.05, * p

    Techniques Used: Sandwich ELISA, Infection, Injection, Isolation, Flow Cytometry, Cytometry, Mouse Assay, Enzyme-linked Immunosorbent Assay

    8) Product Images from "Context-dependent miR-21 regulation of TLR7-mediated autoimmune and foreign antigen driven antibody-forming cell and germinal center responses"

    Article Title: Context-dependent miR-21 regulation of TLR7-mediated autoimmune and foreign antigen driven antibody-forming cell and germinal center responses

    Journal: bioRxiv

    doi: 10.1101/2021.03.12.435182

    miR-21 Drives AFC and GC responses in TLR7-overexpressing SLE-prone mice. All data are from 3 mo old B6. Sle1b .Yaa (Sle1b Yaa ) and Sle1b Yaa miR-21KO male mice. These data are from the analysis of spleens in 2 independent experiments, each with 4 or more mice per group. Each data point represents one mouse, except for HEp-2 quantitation which is detailed below. (A-C) Spleen size and weight, and quantification of splenocytes. ( D ) Flow cytometry analysis of the percentage and number of B220 + GL-7 hi Fas hi GC B cells. ( E ) Flow cytometry quantification of the percentage and number of CD4 + effector T cells and ( F ) number of Tfh cells. ( G ) Histological analysis of the GC structure and ( H ) frequency and size. Scale bars in G represent 1001m. ( I ) Flow cytometry quantification of IgD - CD138 + TACI + plasma cells (PCs). Quantification of ELISpot analysis of autoantibody secreting B cells producing antibodies with reactivity against dsDNA, nucleosome and SmRNP in the ( J ) spleens and ( K ) bone marrow of the indicated genotypes of mice. Quantification and representative images of ELISpot analysis of autoantibody secreting B cells producing antibodies with reactivity against dsDNA and SmRNP in the bone marrow of the indicated genotypes of mice. ( L,M ) IgG and IgG2c serum titers of antibodies to dsDNA, nucleosome and SmRNP, and ( N ) ANA seropositivity in these mice. ( O ) Quantification of the numbers of innate cells and various myeloid cells such as monocytes, moDCs and cDCs. Two group comparison was performed by Mann-Whitney analysis. *p
    Figure Legend Snippet: miR-21 Drives AFC and GC responses in TLR7-overexpressing SLE-prone mice. All data are from 3 mo old B6. Sle1b .Yaa (Sle1b Yaa ) and Sle1b Yaa miR-21KO male mice. These data are from the analysis of spleens in 2 independent experiments, each with 4 or more mice per group. Each data point represents one mouse, except for HEp-2 quantitation which is detailed below. (A-C) Spleen size and weight, and quantification of splenocytes. ( D ) Flow cytometry analysis of the percentage and number of B220 + GL-7 hi Fas hi GC B cells. ( E ) Flow cytometry quantification of the percentage and number of CD4 + effector T cells and ( F ) number of Tfh cells. ( G ) Histological analysis of the GC structure and ( H ) frequency and size. Scale bars in G represent 1001m. ( I ) Flow cytometry quantification of IgD - CD138 + TACI + plasma cells (PCs). Quantification of ELISpot analysis of autoantibody secreting B cells producing antibodies with reactivity against dsDNA, nucleosome and SmRNP in the ( J ) spleens and ( K ) bone marrow of the indicated genotypes of mice. Quantification and representative images of ELISpot analysis of autoantibody secreting B cells producing antibodies with reactivity against dsDNA and SmRNP in the bone marrow of the indicated genotypes of mice. ( L,M ) IgG and IgG2c serum titers of antibodies to dsDNA, nucleosome and SmRNP, and ( N ) ANA seropositivity in these mice. ( O ) Quantification of the numbers of innate cells and various myeloid cells such as monocytes, moDCs and cDCs. Two group comparison was performed by Mann-Whitney analysis. *p

    Techniques Used: Mouse Assay, Quantitation Assay, Flow Cytometry, Enzyme-linked Immunospot, MANN-WHITNEY

    miR-21 Promotes TD-Ag Driven GC Responses. All data presented in panels A-L are from WT and miR-21KO mice at d10 post-immunization from 3 independent experiments with 3-5 mice per group. Except for histology, each data point represents one mouse (biological replicate). (A) Quantification of the number of B cells in the spleen. (B) Representative flow plots of the GC B cell (B220 + GL-7 hi Fas hi ) response. (C-D) Quantification of the (C) percentage GC B cells within the total B220 + population and the (D) total number of GC B cells. (E) Quantification of the number of CD4 T cells in the spleen. (F) Representative flow plots of the follicular CD4 T cell (CXCR5 hi PD-1 hi ) response. (G-H) Quantification of the (G) percentage follicular CD4 T cells within the total CD4 T cell population and the (H) total number of follicular CD4 T cells. ( I ) Representative flow plots for FoxP3 - Tfh and FoxP3 + Tfr cells within the total follicular CD4 T cell population. Total numbers of ( J ) Tfh and ( K ) Tfr cells. (L) Representative images of spleen sections stained for GL-7 (green), CD4 (red), and IgD (blue) and quantification of GC responses by measuring the GC area. Scale bars represent 1001m. Data represent at least 7 mice per genotype. (M-N) Serum antibody titers of NP-specific IgG, IgG1 and IgG2a/c at ( M ) 10d and ( N ) 21d post-immunization with NP-KLH. Two group comparison was performed by Mann-Whitney analysis. ns, not significant, *p
    Figure Legend Snippet: miR-21 Promotes TD-Ag Driven GC Responses. All data presented in panels A-L are from WT and miR-21KO mice at d10 post-immunization from 3 independent experiments with 3-5 mice per group. Except for histology, each data point represents one mouse (biological replicate). (A) Quantification of the number of B cells in the spleen. (B) Representative flow plots of the GC B cell (B220 + GL-7 hi Fas hi ) response. (C-D) Quantification of the (C) percentage GC B cells within the total B220 + population and the (D) total number of GC B cells. (E) Quantification of the number of CD4 T cells in the spleen. (F) Representative flow plots of the follicular CD4 T cell (CXCR5 hi PD-1 hi ) response. (G-H) Quantification of the (G) percentage follicular CD4 T cells within the total CD4 T cell population and the (H) total number of follicular CD4 T cells. ( I ) Representative flow plots for FoxP3 - Tfh and FoxP3 + Tfr cells within the total follicular CD4 T cell population. Total numbers of ( J ) Tfh and ( K ) Tfr cells. (L) Representative images of spleen sections stained for GL-7 (green), CD4 (red), and IgD (blue) and quantification of GC responses by measuring the GC area. Scale bars represent 1001m. Data represent at least 7 mice per genotype. (M-N) Serum antibody titers of NP-specific IgG, IgG1 and IgG2a/c at ( M ) 10d and ( N ) 21d post-immunization with NP-KLH. Two group comparison was performed by Mann-Whitney analysis. ns, not significant, *p

    Techniques Used: Mouse Assay, Staining, MANN-WHITNEY

    9) Product Images from "The Small Rho GTPase TC10 Modulates B Cell Immune Responses"

    Article Title: The Small Rho GTPase TC10 Modulates B Cell Immune Responses

    Journal: The Journal of Immunology Author Choice

    doi: 10.4049/jimmunol.1602167

    Impaired B cell responses in TC10 KO animals. ( A ) Serum was collected from naive WT and TC10 KO animals, and baseline titers of IgM, IgG2b, IgG2c, IgG3, and IgA were measured by sandwich ELISA. ( B – E ) WT and TC10 KO animals were infected with 10
    Figure Legend Snippet: Impaired B cell responses in TC10 KO animals. ( A ) Serum was collected from naive WT and TC10 KO animals, and baseline titers of IgM, IgG2b, IgG2c, IgG3, and IgA were measured by sandwich ELISA. ( B – E ) WT and TC10 KO animals were infected with 10

    Techniques Used: Sandwich ELISA, Infection

    10) Product Images from "Type I interferon signaling is required for the APOBEC3/Rfv3-dependent neutralizing antibody response but not innate retrovirus restriction"

    Article Title: Type I interferon signaling is required for the APOBEC3/Rfv3-dependent neutralizing antibody response but not innate retrovirus restriction

    Journal: Retrovirology

    doi: 10.1186/s12977-017-0349-2

    APOBEC3/Rfv3-dependent NAb response requires type I IFN signaling. Mice were infected with FV/LDV at two different inoculum doses: a , b 10,000 SFFU and c , d 2000 SFFU. a , c Plasma samples at 28 dpi were heat-inactivated and the reciprocal plasma dilution that conferred 50% neutralization was computed. Log 4 -transformed data are shown and used for statistical analyses. b , d FV-specific IgG2b/c titers were determined by endpoint-titration ELISA for mice infected with 10 4 SFFU of FV/LDV. Native FV virions were coated into ELISA plates and twofold dilutions of plasma were added. IgG2b/c antibodies were detected using a combination of anti-IgG2b and anti-IgG2c conjugates. In all panels, each dot corresponds to a mouse and lines correspond to mean values. The total number of mice analyzed was combined from 2 independent experiments. Data were analyzed using 2-tailed unpaired Student’s t test; ns not significant ( p > 0.05)
    Figure Legend Snippet: APOBEC3/Rfv3-dependent NAb response requires type I IFN signaling. Mice were infected with FV/LDV at two different inoculum doses: a , b 10,000 SFFU and c , d 2000 SFFU. a , c Plasma samples at 28 dpi were heat-inactivated and the reciprocal plasma dilution that conferred 50% neutralization was computed. Log 4 -transformed data are shown and used for statistical analyses. b , d FV-specific IgG2b/c titers were determined by endpoint-titration ELISA for mice infected with 10 4 SFFU of FV/LDV. Native FV virions were coated into ELISA plates and twofold dilutions of plasma were added. IgG2b/c antibodies were detected using a combination of anti-IgG2b and anti-IgG2c conjugates. In all panels, each dot corresponds to a mouse and lines correspond to mean values. The total number of mice analyzed was combined from 2 independent experiments. Data were analyzed using 2-tailed unpaired Student’s t test; ns not significant ( p > 0.05)

    Techniques Used: Mouse Assay, Infection, Neutralization, Transformation Assay, Titration, Enzyme-linked Immunosorbent Assay

    11) Product Images from "Deciphering the importance of the palindromic architecture of the immunoglobulin heavy-chain 3' regulatory region"

    Article Title: Deciphering the importance of the palindromic architecture of the immunoglobulin heavy-chain 3' regulatory region

    Journal: Nature Communications

    doi: 10.1038/ncomms10730

    Influence of the 3'RR palindrome on Ig synthesis. ( a ) ELISA analysis of IgG 1 , IgG 2a , IgG 2b , IgG 3 and IgA in supernatants of LPS±IL-4-, INFγ- and TGFβ-stimulated splenocytes of ΔleftPAL, Δ IRIS and wt mice. Data are the mean±s.e.m. of eight experiments with one mouse (8–12 weeks old, male and female). * P
    Figure Legend Snippet: Influence of the 3'RR palindrome on Ig synthesis. ( a ) ELISA analysis of IgG 1 , IgG 2a , IgG 2b , IgG 3 and IgA in supernatants of LPS±IL-4-, INFγ- and TGFβ-stimulated splenocytes of ΔleftPAL, Δ IRIS and wt mice. Data are the mean±s.e.m. of eight experiments with one mouse (8–12 weeks old, male and female). * P

    Techniques Used: Enzyme-linked Immunosorbent Assay, Mouse Assay

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    Immunoprecipitation:

    Article Title: Runx Transcription Factors Repress Human and Murine c-Myc Expression in a DNA-Binding and C-Terminally Dependent Manner
    Article Snippet: Jurkat T cells were sonicated 3 times on ice at 25% power for 10 second pulses and splenocytes were sonicated for 3 times on ice at 30% power for 10 second pulses. .. Chromatin was immunoprecipitated with the control antibody rabbit pre-immune sera, rabbit polyclonal murine anti-distal Runx1 [ ], IgG (Southern Biotech, Birmingham, AL) or a His-probe (AD 1.1.10) antibody (Santa Cruz Biotechnology, Santa Cruz, CA). ..

    other:

    Article Title: IgG subclass responses to excreted-secreted antigens of Plasmodium falciparum in a low-transmission malaria area of the Peruvian Amazon
    Article Snippet: The secondary antibodies were diluted 1:2000 for both IgG1 (HRP-conjugated rabbit anti-human IgG1, Southern Biotech) and IgG3 (HRP-conjugated rabbit anti-human IgG3, Southern Biotech) and were diluted 1:1000 for both IgG2 (HRP-conjugated rabbit anti-human IgG2, Southern Biotech) and IgG4 (HRP-conjugated rabbit anti-human IgG4, Southern Biotech).

    Article Title: Immunization with tegument nucleotidases associated with a subcurative praziquantel treatment reduces worm burden following Schistosoma mansoni challenge
    Article Snippet: 10.7717/peerj.58/supp-2 IgG1 and IgG2a levels induced by immunization with combined (3Teg-Nucl) tegument nucleotidases. (A) Specific IgG1 and IgG2a levels against SmAP before and after challenge. (B) Specific IgG1 and IgG2a levels against SmNPP-5 before and after challenge. (C) Specific IgG1 and IgG2a levels against SmNTPDse before and after challenge.

    Binding Assay:

    Article Title: Channel catfish soluble Fc?R binds conserved linear epitopes present on C?3 and C?4
    Article Snippet: This protocol was also used to assess IpFcRI binding to different mouse immunoglobulins and for these experiments, the latex beads were covalently coupled with mouse IgM, IgG1, IgG2a, IgG2b, IgG3 or IgA (Southern Biotech); mixed with rIpFcRI and stained with fluorescently labeled anti-FLAG M2 mAb as described above. .. IpFcRI binding to reduced Ig was examined by Western blot analyses using normal sera from catfish, rainbow trout, mouse and rabbit and various affinity purified Igs, including catfish IgM, chicken IgY (Aves Labs), rabbit IgG (Southern Biotech) and goat IgG (Southern Biotech). .. Briefly, 0.2 μl of sera or 1 μg of Ig were analyzed under reducing conditions in 10% SDS-PAGE using standard protocols ( ).

    Western Blot:

    Article Title: Channel catfish soluble Fc?R binds conserved linear epitopes present on C?3 and C?4
    Article Snippet: This protocol was also used to assess IpFcRI binding to different mouse immunoglobulins and for these experiments, the latex beads were covalently coupled with mouse IgM, IgG1, IgG2a, IgG2b, IgG3 or IgA (Southern Biotech); mixed with rIpFcRI and stained with fluorescently labeled anti-FLAG M2 mAb as described above. .. IpFcRI binding to reduced Ig was examined by Western blot analyses using normal sera from catfish, rainbow trout, mouse and rabbit and various affinity purified Igs, including catfish IgM, chicken IgY (Aves Labs), rabbit IgG (Southern Biotech) and goat IgG (Southern Biotech). .. Briefly, 0.2 μl of sera or 1 μg of Ig were analyzed under reducing conditions in 10% SDS-PAGE using standard protocols ( ).

    Affinity Purification:

    Article Title: Channel catfish soluble Fc?R binds conserved linear epitopes present on C?3 and C?4
    Article Snippet: This protocol was also used to assess IpFcRI binding to different mouse immunoglobulins and for these experiments, the latex beads were covalently coupled with mouse IgM, IgG1, IgG2a, IgG2b, IgG3 or IgA (Southern Biotech); mixed with rIpFcRI and stained with fluorescently labeled anti-FLAG M2 mAb as described above. .. IpFcRI binding to reduced Ig was examined by Western blot analyses using normal sera from catfish, rainbow trout, mouse and rabbit and various affinity purified Igs, including catfish IgM, chicken IgY (Aves Labs), rabbit IgG (Southern Biotech) and goat IgG (Southern Biotech). .. Briefly, 0.2 μl of sera or 1 μg of Ig were analyzed under reducing conditions in 10% SDS-PAGE using standard protocols ( ).

    Incubation:

    Article Title: IgG Subclass Determines Suppression Versus Enhancement of Humoral Alloimmunity to Kell RBC Antigens in Mice
    Article Snippet: K1 and B6 RBCs were enumerated in peripheral blood and K1 survival was calculated as a ratio of K1:B6 RBCs, as described ( ) Because this procedure normalizes the survival of antigen positive RBCs as a function of wild-type (B6) RBCs injected as a mixture, it removes issues surrounding variability of injection, blood volume, or phlebotomy ( ). .. Assaying Humoral Immune ResponsesSera were incubated with K1, K2, or B6 RBCs followed by APC conjugated anti-mouse IgM or IgG secondary antibodies purchased from Southern Biotechnology (Homewood, AL). ..

    Article Title: Characterization of Serum Antibodies from Women Immunized with Gardasil: A Study of HPV-18 Infection of Primary Human Keratinocytes
    Article Snippet: Data acquisitions were performed on a BD-LSRII flow cytometer (BD Biosciences, San Jose, CA). .. To ascertain that the IgG internalization was Fc-mediated, PHKs were incubated with 25 μg of FITC-labeled, intact rabbit IgG or the F(ab’)2 fragment of IgG (SouthernBiotech, Birmingham, AL) for 3 h at 37°C and analyzed by flow cytometry. .. HPV-18 virions were produced, partially purified and titered as described [ , ].

    Flow Cytometry:

    Article Title: Characterization of Serum Antibodies from Women Immunized with Gardasil: A Study of HPV-18 Infection of Primary Human Keratinocytes
    Article Snippet: Data acquisitions were performed on a BD-LSRII flow cytometer (BD Biosciences, San Jose, CA). .. To ascertain that the IgG internalization was Fc-mediated, PHKs were incubated with 25 μg of FITC-labeled, intact rabbit IgG or the F(ab’)2 fragment of IgG (SouthernBiotech, Birmingham, AL) for 3 h at 37°C and analyzed by flow cytometry. .. HPV-18 virions were produced, partially purified and titered as described [ , ].

    Article Title: Environmental sensing by mature B cells is controlled by the transcription factors PU.1 and SpiB
    Article Snippet: .. Antibodies and flow cytometry Single-cell suspensions were stained with mAb against mouse B220 (RA3-6B2; BD Pharmingen; 1 : 200), IgM (331.12; 1 : 800), IgD (1126 C; 1 : 800), Gr-1 (RB6-8C5; 1 : 800), CD21 (7G6; 1 : 2000), CD23 (B3B4; Biolegend; 1 : 200), IgG1 (X56; BD Pharmingen; 1 : 300), IgG3 (pooled antisera; Southern Biotech; 1 : 250), CD138 (281-2; BD Pharmingen; 1 : 600), FcgR (2.4G2; 1 : 10), BAFF-R (7H22-E16; BD Biosciences; 1 : 100), CD40 (11-0402-86; eBioscience; 1 : 200) and CD98 (RL388; Biolegend; 1 :500). .. Cells were analyzed on FACSCanto flow cytometers and cell sorting was carried out using FACSDiVa or Aria flow cytometers (BD Biosciences).

    Cytometry:

    Article Title: Characterization of Serum Antibodies from Women Immunized with Gardasil: A Study of HPV-18 Infection of Primary Human Keratinocytes
    Article Snippet: Data acquisitions were performed on a BD-LSRII flow cytometer (BD Biosciences, San Jose, CA). .. To ascertain that the IgG internalization was Fc-mediated, PHKs were incubated with 25 μg of FITC-labeled, intact rabbit IgG or the F(ab’)2 fragment of IgG (SouthernBiotech, Birmingham, AL) for 3 h at 37°C and analyzed by flow cytometry. .. HPV-18 virions were produced, partially purified and titered as described [ , ].

    Article Title: Environmental sensing by mature B cells is controlled by the transcription factors PU.1 and SpiB
    Article Snippet: .. Antibodies and flow cytometry Single-cell suspensions were stained with mAb against mouse B220 (RA3-6B2; BD Pharmingen; 1 : 200), IgM (331.12; 1 : 800), IgD (1126 C; 1 : 800), Gr-1 (RB6-8C5; 1 : 800), CD21 (7G6; 1 : 2000), CD23 (B3B4; Biolegend; 1 : 200), IgG1 (X56; BD Pharmingen; 1 : 300), IgG3 (pooled antisera; Southern Biotech; 1 : 250), CD138 (281-2; BD Pharmingen; 1 : 600), FcgR (2.4G2; 1 : 10), BAFF-R (7H22-E16; BD Biosciences; 1 : 100), CD40 (11-0402-86; eBioscience; 1 : 200) and CD98 (RL388; Biolegend; 1 :500). .. Cells were analyzed on FACSCanto flow cytometers and cell sorting was carried out using FACSDiVa or Aria flow cytometers (BD Biosciences).

    Staining:

    Article Title: Environmental sensing by mature B cells is controlled by the transcription factors PU.1 and SpiB
    Article Snippet: .. Antibodies and flow cytometry Single-cell suspensions were stained with mAb against mouse B220 (RA3-6B2; BD Pharmingen; 1 : 200), IgM (331.12; 1 : 800), IgD (1126 C; 1 : 800), Gr-1 (RB6-8C5; 1 : 800), CD21 (7G6; 1 : 2000), CD23 (B3B4; Biolegend; 1 : 200), IgG1 (X56; BD Pharmingen; 1 : 300), IgG3 (pooled antisera; Southern Biotech; 1 : 250), CD138 (281-2; BD Pharmingen; 1 : 600), FcgR (2.4G2; 1 : 10), BAFF-R (7H22-E16; BD Biosciences; 1 : 100), CD40 (11-0402-86; eBioscience; 1 : 200) and CD98 (RL388; Biolegend; 1 :500). .. Cells were analyzed on FACSCanto flow cytometers and cell sorting was carried out using FACSDiVa or Aria flow cytometers (BD Biosciences).

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    SouthernBiotech pe labeled mouse anti rat igg1
    The production of Id + RF by TC.AM14 a mice is correlated with the secretion of anti-chromatin <t>IgG2a</t> a
    Pe Labeled Mouse Anti Rat Igg1, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SouthernBiotech alkaline phosphatase labeled goat anti mouse igg1
    mAb 18B7 modifies the capsule resulting in more 18B7 reactivity. A , flow cytometry gating strategy for Uvitex2b-positive cells, > 99% ( left plot ), used to construct histograms of 18B7-Alexa-568 reactivity cryptococcal capsules following 39 days of incubation with 0 (control), 1, 10, or 50 μg/ml mAb 18B7 (unlabeled) and 50 μg/ml MOPC-31C <t>IgG1</t> control. Histograms shown are from one representative experiment. B , micrograph representations of samples analyzed in A , showing cell wall and capsule stainings. India ink counterstain was used to visualized the capsule under bright field. Scale bar, 10 μm.
    Alkaline Phosphatase Labeled Goat Anti Mouse Igg1, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SouthernBiotech igg2b
    Immunization of ROSA yellow fluorescent protein transgenic (YFP Tg) mice induces vaccine-specific <t>IgG</t> antibodies (a) Total IgG and (b) IgM antibodies specific for inactivated influenza (A/PR8) virus antigen day 9 post prime and boost immunization ( n =
    Igg2b, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SouthernBiotech igg2c hrp
    The LION/repRNA-CoV2S vaccine induces Th1-biased and neutralizing antibodies in C57BL/6 mice. Six to eight-week old C57BL/6 mice (n=5/group) received 10, 1, or 0.1 μg LION/repRNA-CoV2S via the intramuscular route on days 0 and 28. ( A ) Anti-S IgG antibody concentrations were determined by enzyme linked immunosorbent assay (ELISA) on days 14, 28, and 40. For day 14 samples, ( B ) 50% inhibitory concentrations (IC50) were determined by pseudovirus (SARS-CoV-2 Wuhan-Hu-1 pseudotype) neutralization assays. For day 14 samples, ( C ) anti-S IgG1 and <t>IgG2c</t> antibody endpoint titers and ( D ) ratios were determined by ELISA. On day 40, 12 days after a booster immunization, ( E ) spleens and ( F ) lungs were harvested and IFN-γ responses were measured by enzyme-linked immune absorbent spot (ELISpot) assay following an 18-hour stimulation with 10 peptide pools encompassing the S protein and consisting of 15 mers overlapping by 11 amino acids (see Fig. S1). Data in A , C , and D are representative of 3 independent experiments; data in B , E , and F are from a single experiment. Dotted lines in A, B, E, and F represent the lower limit of detection. All data are represented as individual values as well as mean ± s.d. *p
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    The production of Id + RF by TC.AM14 a mice is correlated with the secretion of anti-chromatin IgG2a a

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Activation of rheumatoid factor-specific B cells is antigen-dependent and occurs preferentially outside of germinal centers in the lupus-prone NZM2410 mouse model

    doi: 10.4049/jimmunol.1303000

    Figure Lengend Snippet: The production of Id + RF by TC.AM14 a mice is correlated with the secretion of anti-chromatin IgG2a a

    Article Snippet: The NIM-R6 Ab , which recognizes both CD22a and CD22b alleles ( ) was revealed with either FITC-labeled mouse anti-rat IgG1 (eBioscience) or PE-labeled mouse anti-rat-IgG1 (Southern Biotech).

    Techniques: Mouse Assay

    mAb 18B7 modifies the capsule resulting in more 18B7 reactivity. A , flow cytometry gating strategy for Uvitex2b-positive cells, > 99% ( left plot ), used to construct histograms of 18B7-Alexa-568 reactivity cryptococcal capsules following 39 days of incubation with 0 (control), 1, 10, or 50 μg/ml mAb 18B7 (unlabeled) and 50 μg/ml MOPC-31C IgG1 control. Histograms shown are from one representative experiment. B , micrograph representations of samples analyzed in A , showing cell wall and capsule stainings. India ink counterstain was used to visualized the capsule under bright field. Scale bar, 10 μm.

    Journal: The Journal of Biological Chemistry

    Article Title: A Monoclonal Antibody to Cryptococcus neoformans Glucuronoxylomannan Manifests Hydrolytic Activity for Both Peptides and Polysaccharides *

    doi: 10.1074/jbc.M116.767582

    Figure Lengend Snippet: mAb 18B7 modifies the capsule resulting in more 18B7 reactivity. A , flow cytometry gating strategy for Uvitex2b-positive cells, > 99% ( left plot ), used to construct histograms of 18B7-Alexa-568 reactivity cryptococcal capsules following 39 days of incubation with 0 (control), 1, 10, or 50 μg/ml mAb 18B7 (unlabeled) and 50 μg/ml MOPC-31C IgG1 control. Histograms shown are from one representative experiment. B , micrograph representations of samples analyzed in A , showing cell wall and capsule stainings. India ink counterstain was used to visualized the capsule under bright field. Scale bar, 10 μm.

    Article Snippet: Completion of the assay was performed by successively adding the anti-GXM IgG1 mAb 18B7 at 10 μg/ml and then alkaline phosphatase-labeled goat anti-mouse IgG1 at 1 μg/ml (SouthernBiotech).

    Techniques: Flow Cytometry, Cytometry, Construct, Incubation

    Immunization of ROSA yellow fluorescent protein transgenic (YFP Tg) mice induces vaccine-specific IgG antibodies (a) Total IgG and (b) IgM antibodies specific for inactivated influenza (A/PR8) virus antigen day 9 post prime and boost immunization ( n =

    Journal: Immunology

    Article Title: Distinct B-cell populations contribute to vaccine antigen-specific antibody production in a transgenic mouse model

    doi: 10.1111/imm.12287

    Figure Lengend Snippet: Immunization of ROSA yellow fluorescent protein transgenic (YFP Tg) mice induces vaccine-specific IgG antibodies (a) Total IgG and (b) IgM antibodies specific for inactivated influenza (A/PR8) virus antigen day 9 post prime and boost immunization ( n =

    Article Snippet: It is also interesting to note that IgG2b and IgG2c antibodies were produced at detectable levels in the CD43− (B220+ population) cell fraction of boosted splenocytes, although their levels were lower compared with those in CD43+ cell fractions (Fig. c).

    Techniques: Transgenic Assay, Mouse Assay

    The LION/repRNA-CoV2S vaccine induces Th1-biased and neutralizing antibodies in C57BL/6 mice. Six to eight-week old C57BL/6 mice (n=5/group) received 10, 1, or 0.1 μg LION/repRNA-CoV2S via the intramuscular route on days 0 and 28. ( A ) Anti-S IgG antibody concentrations were determined by enzyme linked immunosorbent assay (ELISA) on days 14, 28, and 40. For day 14 samples, ( B ) 50% inhibitory concentrations (IC50) were determined by pseudovirus (SARS-CoV-2 Wuhan-Hu-1 pseudotype) neutralization assays. For day 14 samples, ( C ) anti-S IgG1 and IgG2c antibody endpoint titers and ( D ) ratios were determined by ELISA. On day 40, 12 days after a booster immunization, ( E ) spleens and ( F ) lungs were harvested and IFN-γ responses were measured by enzyme-linked immune absorbent spot (ELISpot) assay following an 18-hour stimulation with 10 peptide pools encompassing the S protein and consisting of 15 mers overlapping by 11 amino acids (see Fig. S1). Data in A , C , and D are representative of 3 independent experiments; data in B , E , and F are from a single experiment. Dotted lines in A, B, E, and F represent the lower limit of detection. All data are represented as individual values as well as mean ± s.d. *p

    Journal: Science Translational Medicine

    Article Title: An alphavirus-derived replicon RNA vaccine induces SARS-CoV-2 neutralizing antibody and T cell responses in mice and nonhuman primates

    doi: 10.1126/scitranslmed.abc9396

    Figure Lengend Snippet: The LION/repRNA-CoV2S vaccine induces Th1-biased and neutralizing antibodies in C57BL/6 mice. Six to eight-week old C57BL/6 mice (n=5/group) received 10, 1, or 0.1 μg LION/repRNA-CoV2S via the intramuscular route on days 0 and 28. ( A ) Anti-S IgG antibody concentrations were determined by enzyme linked immunosorbent assay (ELISA) on days 14, 28, and 40. For day 14 samples, ( B ) 50% inhibitory concentrations (IC50) were determined by pseudovirus (SARS-CoV-2 Wuhan-Hu-1 pseudotype) neutralization assays. For day 14 samples, ( C ) anti-S IgG1 and IgG2c antibody endpoint titers and ( D ) ratios were determined by ELISA. On day 40, 12 days after a booster immunization, ( E ) spleens and ( F ) lungs were harvested and IFN-γ responses were measured by enzyme-linked immune absorbent spot (ELISpot) assay following an 18-hour stimulation with 10 peptide pools encompassing the S protein and consisting of 15 mers overlapping by 11 amino acids (see Fig. S1). Data in A , C , and D are representative of 3 independent experiments; data in B , E , and F are from a single experiment. Dotted lines in A, B, E, and F represent the lower limit of detection. All data are represented as individual values as well as mean ± s.d. *p

    Article Snippet: Then, in consecutive order, washes in PBS/Tween, serially diluted serum samples, anti-mouse or-monkey IgG, IgG1, IgG2a, or IgG2c-HRP (Southern Biotech) and TMB then HCL were added to the plates.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Neutralization, Enzyme-linked Immunospot