anti eif4ai  (Abcam)

 
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    Name:
    Anti eIF4A1 antibody
    Description:

    Catalog Number:
    ab31217
    Price:
    None
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    Structured Review

    Abcam anti eif4ai
    Western blots of lysates from HEK293-T cells transfected with FMDV O1 Manisa P1 and 3C variants (wild-type strain or L127P or C163A mutant). White arrows indicate unprocessed host protein. Gray arrows indicate processed fragments. (A) Western blots showing FMDV P1 polyprotein and processed products of VP0/VP2, VP3, and VP1. (B) Equal levels of loading indicated by anti-GAPDH antibody analysis. (C to G) Blots of 3C protease host protein targets: (C) anti-NEMO; (D) anti-histone H3; (E) <t>anti-eIF4AI;</t> (F) anti-SAM68 N terminus (N-term); (G) anti-SAM68 C terminus (C-term).

    https://www.bioz.com/result/anti eif4ai/product/Abcam
    Average 94 stars, based on 1527 article reviews
    Price from $9.99 to $1999.99
    anti eif4ai - by Bioz Stars, 2020-09
    94/100 stars

    Images

    1) Product Images from "Foot-and-Mouth Disease (FMD) Virus 3C Protease Mutant L127P: Implications for FMD Vaccine Development"

    Article Title: Foot-and-Mouth Disease (FMD) Virus 3C Protease Mutant L127P: Implications for FMD Vaccine Development

    Journal: Journal of Virology

    doi: 10.1128/JVI.00924-17

    Western blots of lysates from HEK293-T cells transfected with FMDV O1 Manisa P1 and 3C variants (wild-type strain or L127P or C163A mutant). White arrows indicate unprocessed host protein. Gray arrows indicate processed fragments. (A) Western blots showing FMDV P1 polyprotein and processed products of VP0/VP2, VP3, and VP1. (B) Equal levels of loading indicated by anti-GAPDH antibody analysis. (C to G) Blots of 3C protease host protein targets: (C) anti-NEMO; (D) anti-histone H3; (E) anti-eIF4AI; (F) anti-SAM68 N terminus (N-term); (G) anti-SAM68 C terminus (C-term).
    Figure Legend Snippet: Western blots of lysates from HEK293-T cells transfected with FMDV O1 Manisa P1 and 3C variants (wild-type strain or L127P or C163A mutant). White arrows indicate unprocessed host protein. Gray arrows indicate processed fragments. (A) Western blots showing FMDV P1 polyprotein and processed products of VP0/VP2, VP3, and VP1. (B) Equal levels of loading indicated by anti-GAPDH antibody analysis. (C to G) Blots of 3C protease host protein targets: (C) anti-NEMO; (D) anti-histone H3; (E) anti-eIF4AI; (F) anti-SAM68 N terminus (N-term); (G) anti-SAM68 C terminus (C-term).

    Techniques Used: Western Blot, Transfection, Mutagenesis

    Western blot analysis of cell-free reactions with anti-eIF4AI and anti-FLAG antibodies. White arrows indicate unprocessed host protein. Gray arrows indicate processed fragments.
    Figure Legend Snippet: Western blot analysis of cell-free reactions with anti-eIF4AI and anti-FLAG antibodies. White arrows indicate unprocessed host protein. Gray arrows indicate processed fragments.

    Techniques Used: Western Blot

    2) Product Images from "Foot-and-Mouth Disease (FMD) Virus 3C Protease Mutant L127P: Implications for FMD Vaccine Development"

    Article Title: Foot-and-Mouth Disease (FMD) Virus 3C Protease Mutant L127P: Implications for FMD Vaccine Development

    Journal: Journal of Virology

    doi: 10.1128/JVI.00924-17

    Western blots of lysates from HEK293-T cells transfected with FMDV O1 Manisa P1 and 3C variants (wild-type strain or L127P or C163A mutant). White arrows indicate unprocessed host protein. Gray arrows indicate processed fragments. (A) Western blots showing FMDV P1 polyprotein and processed products of VP0/VP2, VP3, and VP1. (B) Equal levels of loading indicated by anti-GAPDH antibody analysis. (C to G) Blots of 3C protease host protein targets: (C) anti-NEMO; (D) anti-histone H3; (E) anti-eIF4AI; (F) anti-SAM68 N terminus (N-term); (G) anti-SAM68 C terminus (C-term).
    Figure Legend Snippet: Western blots of lysates from HEK293-T cells transfected with FMDV O1 Manisa P1 and 3C variants (wild-type strain or L127P or C163A mutant). White arrows indicate unprocessed host protein. Gray arrows indicate processed fragments. (A) Western blots showing FMDV P1 polyprotein and processed products of VP0/VP2, VP3, and VP1. (B) Equal levels of loading indicated by anti-GAPDH antibody analysis. (C to G) Blots of 3C protease host protein targets: (C) anti-NEMO; (D) anti-histone H3; (E) anti-eIF4AI; (F) anti-SAM68 N terminus (N-term); (G) anti-SAM68 C terminus (C-term).

    Techniques Used: Western Blot, Transfection, Mutagenesis

    3) Product Images from "Foot-and-Mouth Disease (FMD) Virus 3C Protease Mutant L127P: Implications for FMD Vaccine Development"

    Article Title: Foot-and-Mouth Disease (FMD) Virus 3C Protease Mutant L127P: Implications for FMD Vaccine Development

    Journal: Journal of Virology

    doi: 10.1128/JVI.00924-17

    Western blot analysis of cell-free reactions with anti-eIF4AI and anti-FLAG antibodies. White arrows indicate unprocessed host protein. Gray arrows indicate processed fragments.
    Figure Legend Snippet: Western blot analysis of cell-free reactions with anti-eIF4AI and anti-FLAG antibodies. White arrows indicate unprocessed host protein. Gray arrows indicate processed fragments.

    Techniques Used: Western Blot

    Related Articles

    Immunohistochemistry:

    Article Title: USP15 Enhances Re-epithelialization Through Deubiquitinating EIF4A1 During Cutaneous Wound Repair
    Article Snippet: .. For the IHC and IF assays, the sections were incubated with primary antibody against USP15 (Abcam, ab4850, 1:200) and EIF4A1 (Abcam, ab31217, 1:200) diluted in blocking solution overnight at 4°C. .. After incubation with horseradish peroxidase-conjugated secondary antibody (IHC) or IF (anti-rabbit, 546 nm; anti-mouse, 488 nm), the sections were counterstained with haematoxylin and developed with diaminobenzidine.

    Immunodetection:

    Article Title: A Novel Target of Action of Minocycline in NGF-Induced Neurite Outgrowth in PC12 Cells: Translation Initiation Factor eIF4AI
    Article Snippet: .. For immunodetection, the blots were blocked for 1 h in TBST (50 mM Tris/HCl, pH 7.8, 0.13 M NaCl, 0.1% Tween 20) containing 5% nonfat dry milk at room temperature (RT), followed by incubation with rabbit anti-eIF4AI antibody (1∶250, ab31217, Abcam, Cambridge, UK) overnight at 4°C in TBST/5% blocker. ..

    Blocking Assay:

    Article Title: USP15 Enhances Re-epithelialization Through Deubiquitinating EIF4A1 During Cutaneous Wound Repair
    Article Snippet: .. For the IHC and IF assays, the sections were incubated with primary antibody against USP15 (Abcam, ab4850, 1:200) and EIF4A1 (Abcam, ab31217, 1:200) diluted in blocking solution overnight at 4°C. .. After incubation with horseradish peroxidase-conjugated secondary antibody (IHC) or IF (anti-rabbit, 546 nm; anti-mouse, 488 nm), the sections were counterstained with haematoxylin and developed with diaminobenzidine.

    Immunoprecipitation:

    Article Title: Antisense oligonucleotides targeting translation inhibitory elements in 5′ UTRs can selectively increase protein levels
    Article Snippet: .. Cell lysates were prepared, and immunoprecipitation was performed using anti-eIF4A antibody (Abcam, ab31217) pre-coated on Protein A/G beads (Millipore). .. After incubation at 4°C for 2 h, beads were washed, and bound RNA was prepared using Trizol (Thermo-Fisher).

    Incubation:

    Article Title: USP15 Enhances Re-epithelialization Through Deubiquitinating EIF4A1 During Cutaneous Wound Repair
    Article Snippet: .. For the IHC and IF assays, the sections were incubated with primary antibody against USP15 (Abcam, ab4850, 1:200) and EIF4A1 (Abcam, ab31217, 1:200) diluted in blocking solution overnight at 4°C. .. After incubation with horseradish peroxidase-conjugated secondary antibody (IHC) or IF (anti-rabbit, 546 nm; anti-mouse, 488 nm), the sections were counterstained with haematoxylin and developed with diaminobenzidine.

    Article Title: A Novel Target of Action of Minocycline in NGF-Induced Neurite Outgrowth in PC12 Cells: Translation Initiation Factor eIF4AI
    Article Snippet: .. For immunodetection, the blots were blocked for 1 h in TBST (50 mM Tris/HCl, pH 7.8, 0.13 M NaCl, 0.1% Tween 20) containing 5% nonfat dry milk at room temperature (RT), followed by incubation with rabbit anti-eIF4AI antibody (1∶250, ab31217, Abcam, Cambridge, UK) overnight at 4°C in TBST/5% blocker. ..

    other:

    Article Title: A cellular response linking eIF4AI activity to eIF4AII transcription
    Article Snippet: Antibodies used in this study were directed against the following: eIF4AI (ab31217; Abcam), eIF4AI (sc-14211; Santa Cruz Biotech), eIF4AII (ab31218; Abcam), eIF4E (sc9976; Santa Cruz Biotech), eIF4G (A300-502A; Bethyl Labs), phospho-eIF2α (44728G; Invitrogen), eIF2α (ab5369; Abcam), PDCD4 (9535; Cell Signaling Tech), human Mcl-1 [S-19] (sc-819; Santa Cruz Biotech), p70-S6Kinase (9202, Cell Signaling Tech), phospho-p70-S6K1 (9205, Cell Signaling), rpS6 (2317, Cell Signaling Tech), phospho-rpS6 (Ser 235/236; 4857, Cell Signaling Tech), GAPDH (ab8245; Abcam), actin (A5441; Sigma), and tubulin (T5168; Sigma).

    Western Blot:

    Article Title: A Novel Target of Action of Minocycline in NGF-Induced Neurite Outgrowth in PC12 Cells: Translation Initiation Factor eIF4AI
    Article Snippet: .. Western blot analysis was performed using rabbit anti-eIF4AI antibody (1∶250, ab31217, Abcam, Cambridge, UK). ..

    Article Title: Foot-and-Mouth Disease (FMD) Virus 3C Protease Mutant L127P: Implications for FMD Vaccine Development
    Article Snippet: .. Western blots of cell-free synthesis products used anti-eIF4AI (ab31217, Abcam) antibody to detect eIF4AI and anti-DYKDDDDK (635691; TaKaRa) antibody to detect FLAG-tagged 3C. .. The P1-2A-2B-3B-3C(L127P) gene construct encoding the FMDV serotype O PanAsia-2 (O/IBD/PAK/10/2010) sequence with flanking ClaI and XbaI restriction enzyme sites was synthesized by GenScript.

    Article Title: Single Nucleoprotein Residue Modulates Arenavirus Replication Complex Formation
    Article Snippet: .. The primary antibodies used for immunofluorescence and Western blot analyses were LCMV NP antibody 1-1.3 , anti-eIF4A1 antibody (ab31217, 1:1000; Abcam), anti-eIF4G antibody (2469, 1:200; Cell Signaling), anti-eIF4E antibody (131480, 1:100; Abcam), anti-PI4P IgM (Z-P004, 1:300; Echelon), antihemagglutinin (anti-HA) antibody (ab130275, 1:100; Abcam), and antiactin antibody (Millipore). .. Anti-mouse Alexa Fluor 488 antibody (A-11029, highly cross-absorbed; Life Technologies) and anti-rabbit Alexa Fluor 594 antibody (A-11037, highly cross-absorbed; Life Technologies) were the secondary antibodies used in immunofluorescence studies.

    Immunofluorescence:

    Article Title: Single Nucleoprotein Residue Modulates Arenavirus Replication Complex Formation
    Article Snippet: .. The primary antibodies used for immunofluorescence and Western blot analyses were LCMV NP antibody 1-1.3 , anti-eIF4A1 antibody (ab31217, 1:1000; Abcam), anti-eIF4G antibody (2469, 1:200; Cell Signaling), anti-eIF4E antibody (131480, 1:100; Abcam), anti-PI4P IgM (Z-P004, 1:300; Echelon), antihemagglutinin (anti-HA) antibody (ab130275, 1:100; Abcam), and antiactin antibody (Millipore). .. Anti-mouse Alexa Fluor 488 antibody (A-11029, highly cross-absorbed; Life Technologies) and anti-rabbit Alexa Fluor 594 antibody (A-11037, highly cross-absorbed; Life Technologies) were the secondary antibodies used in immunofluorescence studies.

    Article Title: Modulation of RNA condensation by the DEAD-box protein eIF4A
    Article Snippet: .. The primary antibodies used for immunofluorescence (IF) include mouse anti-G3BP1 (5 µg/mL, ab56574(Abcam)), rabbit anti-DDX1 (1:100, 11357-1-AP (Proteintech)), and rabbit anti-eIF4A1 (100 µg/mL, ab31217(Abcam)) and the appropriate secondary antibodies used were goat anti-mouse FITC antibody (1:1000, Abcam (ab6785)), and donkey anti-rabbit Alexa Fluor 555 conjugate antibody (1:500, Abcam (ab150062). .. NORAD , PEG3 , and DYNH1C1 smFISH probes were custom made using Stellaris® RNA FISH probe Designer (Biosearch Technologies, Inc., Petaluma, CA).

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    Abcam nf κb iκb kinase kinase ikk β
    Analysis of signaling pathways. (A) Protein levels of NF-κB P65, p-P65, IKKβ and IκBα in the total cell fraction of control and co-cultured Jurkat cells and (B) densitometric analysis of the western blot data. (C) Expression of NF-κB P65 and p-P65 in the nuclear protein of Jurkat cells and (D) densitometric analysis of the western blot data. Levels of NF-κB p-P65 were decreased in the total cell and nuclear fractions of co-cultured Jurkat cells at 72 h. NF-κB, nuclear factor-κB; p-, phosphorylated; IκBα, inhibitor of NF-κBα; <t>IKK,</t> <t>IκB</t> kinase β; J, control; co, co-cultured.
    Nf κb Iκb Kinase Kinase Ikk β, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam anti iκb kinase anti ikk gamma
    Analysis of signaling pathways. (A) Protein levels of NF-κB P65, p-P65, IKKβ and IκBα in the total cell fraction of control and co-cultured Jurkat cells and (B) densitometric analysis of the western blot data. (C) Expression of NF-κB P65 and p-P65 in the nuclear protein of Jurkat cells and (D) densitometric analysis of the western blot data. Levels of NF-κB p-P65 were decreased in the total cell and nuclear fractions of co-cultured Jurkat cells at 72 h. NF-κB, nuclear factor-κB; p-, phosphorylated; IκBα, inhibitor of NF-κBα; <t>IKK,</t> <t>IκB</t> kinase β; J, control; co, co-cultured.
    Anti Iκb Kinase Anti Ikk Gamma, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Abcam κb iκb kinase α
    Analysis of signaling pathways. (A) Protein levels of NF-κB P65, p-P65, IKKβ and IκBα in the total cell fraction of control and co-cultured Jurkat cells and (B) densitometric analysis of the western blot data. (C) Expression of NF-κB P65 and p-P65 in the nuclear protein of Jurkat cells and (D) densitometric analysis of the western blot data. Levels of NF-κB p-P65 were decreased in the total cell and nuclear fractions of co-cultured Jurkat cells at 72 h. NF-κB, nuclear factor-κB; p-, phosphorylated; IκBα, inhibitor of NF-κBα; <t>IKK,</t> <t>IκB</t> kinase β; J, control; co, co-cultured.
    κb Iκb Kinase α, supplied by Abcam, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam iκb kinase
    Analysis of signaling pathways. (A) Protein levels of NF-κB P65, p-P65, IKKβ and IκBα in the total cell fraction of control and co-cultured Jurkat cells and (B) densitometric analysis of the western blot data. (C) Expression of NF-κB P65 and p-P65 in the nuclear protein of Jurkat cells and (D) densitometric analysis of the western blot data. Levels of NF-κB p-P65 were decreased in the total cell and nuclear fractions of co-cultured Jurkat cells at 72 h. NF-κB, nuclear factor-κB; p-, phosphorylated; IκBα, inhibitor of NF-κBα; <t>IKK,</t> <t>IκB</t> kinase β; J, control; co, co-cultured.
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    Image Search Results


    Analysis of signaling pathways. (A) Protein levels of NF-κB P65, p-P65, IKKβ and IκBα in the total cell fraction of control and co-cultured Jurkat cells and (B) densitometric analysis of the western blot data. (C) Expression of NF-κB P65 and p-P65 in the nuclear protein of Jurkat cells and (D) densitometric analysis of the western blot data. Levels of NF-κB p-P65 were decreased in the total cell and nuclear fractions of co-cultured Jurkat cells at 72 h. NF-κB, nuclear factor-κB; p-, phosphorylated; IκBα, inhibitor of NF-κBα; IKK, IκB kinase β; J, control; co, co-cultured.

    Journal: International Journal of Molecular Medicine

    Article Title: Suppressive effect mediated by human adipose-derived stem cells on T cells involves the activation of JNK

    doi: 10.3892/ijmm.2018.3953

    Figure Lengend Snippet: Analysis of signaling pathways. (A) Protein levels of NF-κB P65, p-P65, IKKβ and IκBα in the total cell fraction of control and co-cultured Jurkat cells and (B) densitometric analysis of the western blot data. (C) Expression of NF-κB P65 and p-P65 in the nuclear protein of Jurkat cells and (D) densitometric analysis of the western blot data. Levels of NF-κB p-P65 were decreased in the total cell and nuclear fractions of co-cultured Jurkat cells at 72 h. NF-κB, nuclear factor-κB; p-, phosphorylated; IκBα, inhibitor of NF-κBα; IKK, IκB kinase β; J, control; co, co-cultured.

    Article Snippet: The membranes were probed with primary antibodies against phosphorylated (p)-P65 (1:2,000, cat. no. AF2006, Affinity Biosciences, Cambridge, UK), P65 (1:2,000, cat. no. 10745-1-AP, ProteinTech Group, Inc., Wuhan, China), inhibitor of NF-κB (IκB) kinase (IKK)β (1:1,000, cat. no. Ab124957, Abcam, Cambridge, MA, USA), IκBα (1:1,000, cat. no. 4812, Cell Signaling Technology, Inc., Danvers, MA, USA), B-cell lymphoma 2 (Bcl-2; 1:2,000, cat. no. 12789-1-AP, ProteinTech Group, Inc.), Bcl-2-associated X protein (Bax; 1:5,000, cat. no. 50599-2-IG, Protein Tech Group, Inc), JNK1/2 (1:1,000, cat. no. 9252, Cell Signaling Technology, Inc.), p-JNK1/2 (1:1,000, cat. no. 4668, Cell Signaling Technology, Inc.), extracellular signal-regulated kinase (ERK)1/2 (1:1,000, cat. no. 9102, Cell Signaling Technology, Inc.), p-ERK1/2 (1:2,000, cat. no. 4370, Cell Signaling Technology, Inc.), P38 (1:1,000, cat. no. 9212, Cell Signaling, Technology, Inc.), p-P38 (1:1,000, cat. no. 9211, Cell Signaling Technology, Inc.), mothers against decapentaplegic (Smad)2/3 (1:1,000, cat. no. AF6367, Affinity Biosciences), p-Smad2/3 (1:200, cat. no. MAB8935, R & D Systems, Inc.) and the loading control β-actin (1:200, cat. no. BM0627, Boster Biological Technology, Wuhan, China).

    Techniques: Cell Culture, Western Blot, Expressing