Journal: Bioengineering & Translational Medicine

Article Title: Self‐assembly of PEG – PPS polymers and LL ‐37 peptide nanomicelles improves the oxidative microenvironment and promotes angiogenesis to facilitate chronic wound healing

doi: 10.1002/btm2.10619

Figure Lengend Snippet: Immunohistochemical staining of the inflammatory factors TNF‐α and IL‐1β in diabetic wounds. (a) Immunohistochemical staining of TNF‐α in diabetic wounds under different treatments. (b) Immunohistochemical staining of IL‐1β of diabetic wounds under different treatments. (c) Statistical analysis of the percentage of TNF‐α‐ and IL‐1β‐positive area in wound tissue ( n = 4). The data are expressed as mean ± SD. * p < 0.05, ** p < 0.01, n.s., no significance (one‐way ANOVA). IL, interleukin; TNF, tumor necrosis factor.

Article Snippet: After first incubating the tissue sections with primary antibodies (rabbit anti‐mouse CD31 [1:50, ABclonal, Cat# A0378], anti‐interleukin (IL)‐1 beta rabbit pAb [1:800, Servicebio, GB11113‐100], and tumor necrosis factor (TNF) Alpha [1:400, Proteintech, Cat No. 60291‐1‐lg]) at 4°C for 24 h, the sections were incubated with biotin‐labeled secondary antibody and horseradish enzyme‐labeled streptomyces at room temperature for 2 h. The color reaction was performed using diaminobenzidine (DAB) kit.

Techniques: Immunohistochemistry, Staining

Journal: Journal of Diabetes

Article Title: HLA‐DR3 mediated CD4 T cell response against GAD65 in type 1 diabetes patients

doi: 10.1111/1753-0407.13406

Figure Lengend Snippet: Stimulation indices of CD4 T cells expressing IFN‐γ, TNF‐α, IL‐17, and IL‐10 in response to stimulation with four different GAD65 peptide pools (PP1, PP2, PP3, and PP4) in type 1 diabetes patients and their comparison with that in healthy controls. The results are expressed as mean ± SEM. IFN‐γ, interferon gamma; IL, interleukin; PP, peptide pool; PP1–4 IFN‐γ, PP‐stimulated CD4 T cells expressing IFN‐γ; PP1–4 TNF‐α, PP‐stimulated CD4 T cells expressing TNF‐α; PP1–4 IL‐17, PP‐stimulated CD4 T cells expressing IL‐17; PP1–4 IL‐10, PP‐stimulated CD4 T cells expressing IL‐10; TNF‐α, tumor necrosis factor alpha

Article Snippet: The intracellular cytokine staining was performed on permeabilized and fixed cells with BV480 mouse anti‐human interferon‐gamma (IFN‐γ; 566100, BD Biosciences, USA), APC mouse anti‐human tumor necrosis factor‐alpha (TNF‐α; 554514, BD Biosciences, USA), PE mouse anti‐human IL‐17A (560486, BD Biosciences, USA), and BV421 rat anti‐human and viral IL‐10 (564053, BD Biosciences, USA) antibodies.

Techniques: Expressing

Journal: Journal of Diabetes

Article Title: HLA‐DR3 mediated CD4 T cell response against GAD65 in type 1 diabetes patients

doi: 10.1111/1753-0407.13406

Figure Lengend Snippet: Multiple comparison (one‐way analysis of variance) analysis of stimulation indices of CD4 T cells expressing IFN‐γ (blue), TNF‐α (brown), IL‐17 (green), and IL‐10 (violet) following stimulation with four peptide pools (PP 1–4) in T1D patients (A) and healthy controls (B). The results are expressed as mean ± SEM. IFN‐γ, interferon gamma; IL, interleukin; PP, peptide pool; T1D, type 1 diabetes; TNF‐α, tumor necrosis factor alpha.

Article Snippet: The intracellular cytokine staining was performed on permeabilized and fixed cells with BV480 mouse anti‐human interferon‐gamma (IFN‐γ; 566100, BD Biosciences, USA), APC mouse anti‐human tumor necrosis factor‐alpha (TNF‐α; 554514, BD Biosciences, USA), PE mouse anti‐human IL‐17A (560486, BD Biosciences, USA), and BV421 rat anti‐human and viral IL‐10 (564053, BD Biosciences, USA) antibodies.

Techniques: Expressing

Journal: Journal of Diabetes

Article Title: HLA‐DR3 mediated CD4 T cell response against GAD65 in type 1 diabetes patients

doi: 10.1111/1753-0407.13406

Figure Lengend Snippet: Stimulation indices of CD4 T cells expressing IFN‐γ, TNF‐α, IL‐17, and IL‐10 cytokines in response to stimulation with four different GAD65 peptide pools (PP1, PP2, PP3, and PP4) in HLA‐DRB1*03‐DQA1‐DQB1*02 positive vs negative patients. The results are expressed as mean ± SEM. IFN‐γ, interferon gamma; IL, interleukin; PP, peptide pool; T1D, type 1 diabetes; TNF‐α, tumor necrosis factor alpha.

Article Snippet: The intracellular cytokine staining was performed on permeabilized and fixed cells with BV480 mouse anti‐human interferon‐gamma (IFN‐γ; 566100, BD Biosciences, USA), APC mouse anti‐human tumor necrosis factor‐alpha (TNF‐α; 554514, BD Biosciences, USA), PE mouse anti‐human IL‐17A (560486, BD Biosciences, USA), and BV421 rat anti‐human and viral IL‐10 (564053, BD Biosciences, USA) antibodies.

Techniques: Expressing

Journal: Journal of Diabetes

Article Title: HLA‐DR3 mediated CD4 T cell response against GAD65 in type 1 diabetes patients

doi: 10.1111/1753-0407.13406

Figure Lengend Snippet: Stimulation indices of CD4 T cells expressing IFN‐γ, TNF‐α, IL‐17, and IL‐10 cytokines in response to stimulation with four different GAD65 peptide pools (PP1, PP2, PP3, and PP4) in HLA‐DRB1*03‐DQA1*05‐DQB1*02 positive vs negative healthy controls. The results are expressed as mean ± SEM. HC, healthy controls; IFN‐γ, interferon gamma; IL, interleukin; PP, peptide pool; TNF‐α, tumor necrosis factor alpha.

Article Snippet: The intracellular cytokine staining was performed on permeabilized and fixed cells with BV480 mouse anti‐human interferon‐gamma (IFN‐γ; 566100, BD Biosciences, USA), APC mouse anti‐human tumor necrosis factor‐alpha (TNF‐α; 554514, BD Biosciences, USA), PE mouse anti‐human IL‐17A (560486, BD Biosciences, USA), and BV421 rat anti‐human and viral IL‐10 (564053, BD Biosciences, USA) antibodies.

Techniques: Expressing

Journal: Journal of Diabetes

Article Title: HLA‐DR3 mediated CD4 T cell response against GAD65 in type 1 diabetes patients

doi: 10.1111/1753-0407.13406

Figure Lengend Snippet: Stimulation indices of CD4 T cells expressing IFN‐γ, TNF‐α, IL‐17, and IL‐10 cytokines in response to stimulation with four different GAD65 peptide pools (PP1, PP2, PP3, and PP4) in HLA‐DRB1*03‐DQA1*05‐DQB1*02 positive T1D patients and HLA‐DRB1*03‐DQA1*05‐DQB1*02 positive healthy controls. The results are expressed as mean ± SEM. HC, healthy controls; IFN‐γ, interferon gamma; IL, interleukin; PP, peptide pool; SEM, standard error of mean; T1D, type 1 diabetes; TNF‐α, tumor necrosis factor alpha.

Article Snippet: The intracellular cytokine staining was performed on permeabilized and fixed cells with BV480 mouse anti‐human interferon‐gamma (IFN‐γ; 566100, BD Biosciences, USA), APC mouse anti‐human tumor necrosis factor‐alpha (TNF‐α; 554514, BD Biosciences, USA), PE mouse anti‐human IL‐17A (560486, BD Biosciences, USA), and BV421 rat anti‐human and viral IL‐10 (564053, BD Biosciences, USA) antibodies.

Techniques: Expressing

Journal: Journal of Diabetes

Article Title: HLA‐DR3 mediated CD4 T cell response against GAD65 in type 1 diabetes patients

doi: 10.1111/1753-0407.13406

Figure Lengend Snippet: Ratios of stimulation indices for IFN‐γ/IL‐10 when CD4 T cells were stimulated with four different GAD65 peptide pools (PP1, PP2, PP3, and PP4) in HLA‐DRB1*03‐DQA1*05‐DQB1*02 positive vs negative T1D patients, HLA‐DRB1*03‐DQA1*05‐DQB1*02 positive vs negative healthy controls and HLA‐DRB1*03‐DQA1*05‐DQB1*02 positive T1D vs HLA‐DRB1*03‐DQA1*05‐DQB1*02 positive healthy controls. The results are expressed as mean ± SEM. HC, healthy controls; IFN‐γ, interferon gamma; IL, interleukin; PP, peptide pool; SEM, standard error of mean; T1D, type 1 diabetes; TNF‐α, tumor necrosis factor alpha.

Article Snippet: The intracellular cytokine staining was performed on permeabilized and fixed cells with BV480 mouse anti‐human interferon‐gamma (IFN‐γ; 566100, BD Biosciences, USA), APC mouse anti‐human tumor necrosis factor‐alpha (TNF‐α; 554514, BD Biosciences, USA), PE mouse anti‐human IL‐17A (560486, BD Biosciences, USA), and BV421 rat anti‐human and viral IL‐10 (564053, BD Biosciences, USA) antibodies.

Techniques:

Journal: Journal of Diabetes

Article Title: HLA‐DR3 mediated CD4 T cell response against GAD65 in type 1 diabetes patients

doi: 10.1111/1753-0407.13406

Figure Lengend Snippet: Ratios of stimulation indices for IL‐17/IL‐10 when the CD4 T cells were stimulated with four different GAD65 peptide pools (PP1, PP2, PP3, and PP4) in HLA‐DRB1*03‐DQA1*05‐DQB1*02 positive vs negative T1D patients, HLA‐DRB1*03‐DQA1*05‐DQB1*02 positive vs negative healthy controls and HLA‐DRB1*03‐DQA1*05‐DQB1*02 positive T1D vs HLA‐DRB1*03‐DQA1*05‐DQB1*02 positive healthy controls. The results are expressed as mean ± SEM. HC, healthy controls; IFN‐γ, interferon gamma; IL, interleukin; PP, peptide pool; SEM, standard error of mean; T1D, type 1 diabetes; TNF‐α, tumor necrosis factor alpha.

Article Snippet: The intracellular cytokine staining was performed on permeabilized and fixed cells with BV480 mouse anti‐human interferon‐gamma (IFN‐γ; 566100, BD Biosciences, USA), APC mouse anti‐human tumor necrosis factor‐alpha (TNF‐α; 554514, BD Biosciences, USA), PE mouse anti‐human IL‐17A (560486, BD Biosciences, USA), and BV421 rat anti‐human and viral IL‐10 (564053, BD Biosciences, USA) antibodies.

Techniques:

Journal: Journal of Diabetes

Article Title: HLA‐DR3 mediated CD4 T cell response against GAD65 in type 1 diabetes patients

doi: 10.1111/1753-0407.13406

Figure Lengend Snippet: Stimulation indices of CD4 T cells' expression of IFN‐γ, TNF‐α, IL‐17, and IL‐10 when the cells were stimulated with four different GAD65 peptide pools (PP1, PP2, PP3, and PP4) in recently diagnosed and long‐standing T1D patients. The results are represented by box and whisker plots where boxes represent mean ± SEM and whiskers represent 10–90 percentile data range. IFN‐γ, interferon gamma; IL, interleukin; PP, peptide pool; T1D, type 1 diabetes; TNF‐α, tumor necrosis factor alpha.

Article Snippet: The intracellular cytokine staining was performed on permeabilized and fixed cells with BV480 mouse anti‐human interferon‐gamma (IFN‐γ; 566100, BD Biosciences, USA), APC mouse anti‐human tumor necrosis factor‐alpha (TNF‐α; 554514, BD Biosciences, USA), PE mouse anti‐human IL‐17A (560486, BD Biosciences, USA), and BV421 rat anti‐human and viral IL‐10 (564053, BD Biosciences, USA) antibodies.

Techniques: Expressing, Whisker Assay

Journal: Journal of Diabetes

Article Title: HLA‐DR3 mediated CD4 T cell response against GAD65 in type 1 diabetes patients

doi: 10.1111/1753-0407.13406

Figure Lengend Snippet: Stimulation indices of CD4 T cells' expression of IFN‐γ, TNF‐α, IL‐17, and IL‐10 when the cells were stimulated with four different GAD65 peptide pools (PP1, PP2, PP3, and PP4) in recently diagnosed and long‐standing HLA‐DRB1*03‐DQA1*05‐DQB1*02 positive T1D patients. The results are represented by box and whisker plots where boxes represent mean ± SEM and whiskers represent 10–90 percentile data range. IFN‐γ, interferon gamma; IL, interleukin; PP, peptide pool; T1D, type 1 diabetes; TNF‐α, tumor necrosis factor alpha.

Article Snippet: The intracellular cytokine staining was performed on permeabilized and fixed cells with BV480 mouse anti‐human interferon‐gamma (IFN‐γ; 566100, BD Biosciences, USA), APC mouse anti‐human tumor necrosis factor‐alpha (TNF‐α; 554514, BD Biosciences, USA), PE mouse anti‐human IL‐17A (560486, BD Biosciences, USA), and BV421 rat anti‐human and viral IL‐10 (564053, BD Biosciences, USA) antibodies.

Techniques: Expressing, Whisker Assay