Journal:

Article Title: Degranulation of Paneth Cells via Toll-Like Receptor 9

doi:

Figure Lengend Snippet: Western blot analysis of murine B lymphocyte and EL-4 cell lysates (right) and human HT-29 and HEK293 cell lysates (left) using antibodies 5G5 and N-15 against murine TLR9 and human TLR9, respectively.

Article Snippet: Murine biotinylated anti-murine TLR9 monoclonal antibody (mAb) 5G5 was purchased from Hycult Biotechnology b.v. (Uden, The Netherlands).

Techniques: Western Blot

Journal:

Article Title: Degranulation of Paneth Cells via Toll-Like Receptor 9

doi:

Figure Lengend Snippet: Interference contrast images of villi longitudinal sections from murine jejunum immunostained with anti-TLR9 mAb 5G5 show expression of TLR9 in cytoplasm of enterocytes (A) and in Paneth cells (B). Confocal microscope images of TLR9 expression in enterocytes and Paneth cells (C) with granules (arrowhead) clearly labeled (D). Red, TLR9 staining; green, Yopro-3 nuclear staining. Scale bars: 10 μm (A, B); 30 μm (C); 8 μm (D).

Article Snippet: Murine biotinylated anti-murine TLR9 monoclonal antibody (mAb) 5G5 was purchased from Hycult Biotechnology b.v. (Uden, The Netherlands).

Techniques: Expressing, Microscopy, Labeling, Staining

Journal:

Article Title: Degranulation of Paneth Cells via Toll-Like Receptor 9

doi:

Figure Lengend Snippet: Photomicrographs of transverse section of human ileum. Enterocytes labeled with N-15 antibody against TLR9 (A). Detail of the deep portion of ileum mucosa with Paneth cells strongly labeled (B). Scale bars, 10 μm.

Article Snippet: Murine biotinylated anti-murine TLR9 monoclonal antibody (mAb) 5G5 was purchased from Hycult Biotechnology b.v. (Uden, The Netherlands).

Techniques: Labeling

Journal:

Article Title: Degranulation of Paneth Cells via Toll-Like Receptor 9

doi:

Figure Lengend Snippet: Immunohistochemical reactivity with TLR9 mAb of jejunum epithelial cells from mice 24 hours previously injected intraperitoneally with 1668 CpG-ODN (A) or control AP1-ODN (B) or left untreated (C). D–F: Confocal images of Paneth cells of jejunum from mice 24 hours previously treated with CpG-ODN or AP1-ODN or left untreated, respectively. Scale bars: 10 μm (A–C); 17 μm (D–F).

Article Snippet: Murine biotinylated anti-murine TLR9 monoclonal antibody (mAb) 5G5 was purchased from Hycult Biotechnology b.v. (Uden, The Netherlands).

Techniques: Immunohistochemical staining, Injection