tlr9 a405  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    R&D Systems tlr9 a405
    Tlr9 A405, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlr9 a405/product/R&D Systems
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tlr9 a405 - by Bioz Stars, 2024-07
    94/100 stars

    Images

    tlr9  (OriGene)


    Bioz Verified Symbol OriGene is a verified supplier
    Bioz Manufacturer Symbol OriGene manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92

    Structured Review

    OriGene tlr9
    Sections of nasal biopsies were incubated with antibodies against TLR3 ( A ), TLR7 ( B ), <t>TLR9</t> ( C ), RIG-I ( D ), and MDA-5 ( E ) and visualized by 3, 3′-diaminobenzidine (brown). In control slides ( F ), N-series universal negative control reagent was used. All sections were accompanied with a square magnification. All slides were counterstained with haematoxylin (blue). The figure shows one representative biopsy out of four (3 male, 1 female). The arrows indicate positive stained cells.
    Tlr9, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlr9/product/OriGene
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tlr9 - by Bioz Stars, 2024-07
    92/100 stars

    Images

    1) Product Images from "Functional Effects of Toll-Like Receptor (TLR)3, 7, 9, RIG-I and MDA-5 Stimulation in Nasal Epithelial Cells"

    Article Title: Functional Effects of Toll-Like Receptor (TLR)3, 7, 9, RIG-I and MDA-5 Stimulation in Nasal Epithelial Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0098239

    Sections of nasal biopsies were incubated with antibodies against TLR3 ( A ), TLR7 ( B ), TLR9 ( C ), RIG-I ( D ), and MDA-5 ( E ) and visualized by 3, 3′-diaminobenzidine (brown). In control slides ( F ), N-series universal negative control reagent was used. All sections were accompanied with a square magnification. All slides were counterstained with haematoxylin (blue). The figure shows one representative biopsy out of four (3 male, 1 female). The arrows indicate positive stained cells.
    Figure Legend Snippet: Sections of nasal biopsies were incubated with antibodies against TLR3 ( A ), TLR7 ( B ), TLR9 ( C ), RIG-I ( D ), and MDA-5 ( E ) and visualized by 3, 3′-diaminobenzidine (brown). In control slides ( F ), N-series universal negative control reagent was used. All sections were accompanied with a square magnification. All slides were counterstained with haematoxylin (blue). The figure shows one representative biopsy out of four (3 male, 1 female). The arrows indicate positive stained cells.

    Techniques Used: Incubation, Negative Control, Staining

    Epithelial cells from primary HNEC ( A–E ), Detroit-562 ( G–K ) and FaDu ( M–Q ) were incubated with antibodies against TLR3, TLR7, TLR9, RIG-I, and MDA-5 and visualized by 3, 3′-diaminobenzidine (brown). In controls, N-series universal negative control reagent was used ( F, L, R ). All cells were counterstained with haematoxylin (blue). The figure shows one representative staining out of three independent experiments. The markers in the figure are 50 µm.
    Figure Legend Snippet: Epithelial cells from primary HNEC ( A–E ), Detroit-562 ( G–K ) and FaDu ( M–Q ) were incubated with antibodies against TLR3, TLR7, TLR9, RIG-I, and MDA-5 and visualized by 3, 3′-diaminobenzidine (brown). In controls, N-series universal negative control reagent was used ( F, L, R ). All cells were counterstained with haematoxylin (blue). The figure shows one representative staining out of three independent experiments. The markers in the figure are 50 µm.

    Techniques Used: Incubation, Negative Control, Staining

    HNEC ( A ), Detroit-562 ( B ) and FaDu ( C ) were stained intracellularly with Abs against TLR3, TLR7, TLR9, RIG-I and MDA-5 (open histograms) or appropriate isotype control (shaded histograms) and analyzed by flow cytometry. Representative pictures from one out of three independent experiments are shown.
    Figure Legend Snippet: HNEC ( A ), Detroit-562 ( B ) and FaDu ( C ) were stained intracellularly with Abs against TLR3, TLR7, TLR9, RIG-I and MDA-5 (open histograms) or appropriate isotype control (shaded histograms) and analyzed by flow cytometry. Representative pictures from one out of three independent experiments are shown.

    Techniques Used: Staining, Flow Cytometry

    Nasal biopsies and epithelial cells were cultured in the absence (Untreated) or presence of 10 µg/ml poly(I:C) (TLR3), 10 µg/ml R-837 (TLR7), 1 µM CpG (TLR9) and 1 µg/ml poly(I:C)/LyoVec (RIG-I/MDA-5). TNF-α (10 ng/ml) was used as a positive control (data not shown). After 24 h, supernatants from nasal biopsies (n = 5) ( A–D ), HNEC (n = 6–9) ( E–H ), Detroit-562 (n = 5) ( I–L ) and FaDu (n = 5–9) ( M–P ) were collected and analyzed for levels of IL-6, IL-8, GM-CSF and IFN-β using ELISA. Data is presented as mean ± SEM of 5 to 9 independent experiments. *, p<0.05; **, p<0.01; ***, p<0.001.
    Figure Legend Snippet: Nasal biopsies and epithelial cells were cultured in the absence (Untreated) or presence of 10 µg/ml poly(I:C) (TLR3), 10 µg/ml R-837 (TLR7), 1 µM CpG (TLR9) and 1 µg/ml poly(I:C)/LyoVec (RIG-I/MDA-5). TNF-α (10 ng/ml) was used as a positive control (data not shown). After 24 h, supernatants from nasal biopsies (n = 5) ( A–D ), HNEC (n = 6–9) ( E–H ), Detroit-562 (n = 5) ( I–L ) and FaDu (n = 5–9) ( M–P ) were collected and analyzed for levels of IL-6, IL-8, GM-CSF and IFN-β using ELISA. Data is presented as mean ± SEM of 5 to 9 independent experiments. *, p<0.05; **, p<0.01; ***, p<0.001.

    Techniques Used: Cell Culture, Positive Control, Enzyme-linked Immunosorbent Assay


    Structured Review

    Becton Dickinson anti human tlr9 pe
    Anti Human Tlr9 Pe, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human tlr9 pe/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti human tlr9 pe - by Bioz Stars, 2024-07
    86/100 stars

    Images

    tlr9 a405  (R&D Systems)


    Bioz Verified Symbol R&D Systems is a verified supplier
    Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    R&D Systems tlr9 a405
    Tlr9 A405, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlr9 a405/product/R&D Systems
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tlr9 a405 - by Bioz Stars, 2024-07
    94/100 stars

    Images

    anti human cd3 antibody tlr9 agonist odn2006  (Thermo Fisher)


    Bioz Verified Symbol Thermo Fisher is a verified supplier
    Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Thermo Fisher anti human cd3 antibody tlr9 agonist odn2006
    Anti Human Cd3 Antibody Tlr9 Agonist Odn2006, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human cd3 antibody tlr9 agonist odn2006/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti human cd3 antibody tlr9 agonist odn2006 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    rabbit polyclonal anti human tlr9 antibody  (Danaher Inc)


    Bioz Verified Symbol Danaher Inc is a verified supplier
    Bioz Manufacturer Symbol Danaher Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Danaher Inc rabbit polyclonal anti human tlr9 antibody
    Rabbit Polyclonal Anti Human Tlr9 Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti human tlr9 antibody/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti human tlr9 antibody - by Bioz Stars, 2024-07
    86/100 stars

    Images

    anti human tlr9 pe  (Thermo Fisher)


    Bioz Verified Symbol Thermo Fisher is a verified supplier
    Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Thermo Fisher anti human tlr9 pe
    Primers Sequences and the Size of Amplified Fragments for Toll-Like Receptors
    Anti Human Tlr9 Pe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human tlr9 pe/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti human tlr9 pe - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "T Lymphocyte Subsets Profile and Toll-Like Receptors Responses in Patients with Herpes Zoster"

    Article Title: T Lymphocyte Subsets Profile and Toll-Like Receptors Responses in Patients with Herpes Zoster

    Journal: Journal of Pain Research

    doi: 10.2147/JPR.S405157

    Primers Sequences and the Size of Amplified Fragments for Toll-Like Receptors
    Figure Legend Snippet: Primers Sequences and the Size of Amplified Fragments for Toll-Like Receptors

    Techniques Used: Amplification

    The mRNA expression of toll-like receptors in herpes zoster patients (HZ) and control groups (NC), including TLR2 ( a ), TLR4 ( b ), TLR7 ( c ), and TLR9 ( d ). Data were presented as mean±SD and the significance was analyzed by Student’s t -test, * P <0.05, ** P <0.01.
    Figure Legend Snippet: The mRNA expression of toll-like receptors in herpes zoster patients (HZ) and control groups (NC), including TLR2 ( a ), TLR4 ( b ), TLR7 ( c ), and TLR9 ( d ). Data were presented as mean±SD and the significance was analyzed by Student’s t -test, * P <0.05, ** P <0.01.

    Techniques Used: Expressing

    rabbit anti human tlr9 monoclonal antibody  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc rabbit anti human tlr9 monoclonal antibody
    Expressions of <t>TLR9</t> in chronic eczema lesions and the perilesional normal skin at the edge (SP *400). ( a ) Epidermic of skin lesion. ( b ) Dermis of skin lesion. ( c ) Perilesional normal skin. ( a ) A few TLR9+ cells with brown cytoplasm were observed in the epidermal of chronic eczema lesions. ( b ) A few TLR9+ cells with brown cytoplasm were observed in the dermal papillary layer of chronic eczema lesion. ( c ) Few TLR9+ cells with brown cytoplasm were observed in both epidermal and papillary layer of perilesional normal skin of the lesion.
    Rabbit Anti Human Tlr9 Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti human tlr9 monoclonal antibody/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti human tlr9 monoclonal antibody - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "The Expression of Plasmacytoid Dendritic Cells and TLR7/9-MyD88-IRAKs Pathway in Chronic Eczema Lesions"

    Article Title: The Expression of Plasmacytoid Dendritic Cells and TLR7/9-MyD88-IRAKs Pathway in Chronic Eczema Lesions

    Journal: Clinical, Cosmetic and Investigational Dermatology

    doi: 10.2147/CCID.S405491

    Expressions of TLR9 in chronic eczema lesions and the perilesional normal skin at the edge (SP *400). ( a ) Epidermic of skin lesion. ( b ) Dermis of skin lesion. ( c ) Perilesional normal skin. ( a ) A few TLR9+ cells with brown cytoplasm were observed in the epidermal of chronic eczema lesions. ( b ) A few TLR9+ cells with brown cytoplasm were observed in the dermal papillary layer of chronic eczema lesion. ( c ) Few TLR9+ cells with brown cytoplasm were observed in both epidermal and papillary layer of perilesional normal skin of the lesion.
    Figure Legend Snippet: Expressions of TLR9 in chronic eczema lesions and the perilesional normal skin at the edge (SP *400). ( a ) Epidermic of skin lesion. ( b ) Dermis of skin lesion. ( c ) Perilesional normal skin. ( a ) A few TLR9+ cells with brown cytoplasm were observed in the epidermal of chronic eczema lesions. ( b ) A few TLR9+ cells with brown cytoplasm were observed in the dermal papillary layer of chronic eczema lesion. ( c ) Few TLR9+ cells with brown cytoplasm were observed in both epidermal and papillary layer of perilesional normal skin of the lesion.

    Techniques Used:

    The Positive Rate and Intensity of  TLR9  Expression in 16 Cases of Chronic Eczema and 14 Cases of Normal Skin Around Eczema
    Figure Legend Snippet: The Positive Rate and Intensity of TLR9 Expression in 16 Cases of Chronic Eczema and 14 Cases of Normal Skin Around Eczema

    Techniques Used: Expressing

    rabbit polyclonal anti human tlr9 antibody  (Abcam)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Abcam rabbit polyclonal anti human tlr9 antibody
    Antibodies and methods selected for the immunohistochemical study
    Rabbit Polyclonal Anti Human Tlr9 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti human tlr9 antibody/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti human tlr9 antibody - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Involvement of TLR9 in priming the immune response in oral papillomatosis induced by low-risk HPV"

    Article Title: Involvement of TLR9 in priming the immune response in oral papillomatosis induced by low-risk HPV

    Journal: Romanian Journal of Morphology and Embryology

    doi: 10.47162/RJME.64.2.08

    Antibodies and methods selected for the immunohistochemical study
    Figure Legend Snippet: Antibodies and methods selected for the immunohistochemical study

    Techniques Used: Immunohistochemistry, Amplification, Plasmid Preparation

    HPV-negative oral papillomatosis: (a–d) Immunoreaction for TLR9; (e–h) Immunoreaction for CD68. (a–d, g, and h) ×200; (f) ×100. CD68: Cluster of differentiation 68; TLR9: Toll-like receptor 9.
    Figure Legend Snippet: HPV-negative oral papillomatosis: (a–d) Immunoreaction for TLR9; (e–h) Immunoreaction for CD68. (a–d, g, and h) ×200; (f) ×100. CD68: Cluster of differentiation 68; TLR9: Toll-like receptor 9.

    Techniques Used:

    Low-risk HPV-positive oral papillomatosis: (a–d) Immunoreaction for TLR9; (e–h) Immunoreaction for CD68. (a–g) ×200; (h) ×45.
    Figure Legend Snippet: Low-risk HPV-positive oral papillomatosis: (a–d) Immunoreaction for TLR9; (e–h) Immunoreaction for CD68. (a–g) ×200; (h) ×45.

    Techniques Used:

    Spearman’s correlation between the macrophage number and other variables of  TLR9  immunohistochemical signal
    Figure Legend Snippet: Spearman’s correlation between the macrophage number and other variables of TLR9 immunohistochemical signal

    Techniques Used: Immunohistochemistry

    tlr9  (OriGene)


    Bioz Verified Symbol OriGene is a verified supplier
    Bioz Manufacturer Symbol OriGene manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92

    Structured Review

    OriGene tlr9
    Sections of nasal biopsies were incubated with antibodies against TLR3 ( A ), TLR7 ( B ), <t>TLR9</t> ( C ), RIG-I ( D ), and MDA-5 ( E ) and visualized by 3, 3′-diaminobenzidine (brown). In control slides ( F ), N-series universal negative control reagent was used. All sections were accompanied with a square magnification. All slides were counterstained with haematoxylin (blue). The figure shows one representative biopsy out of four (3 male, 1 female). The arrows indicate positive stained cells.
    Tlr9, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlr9/product/OriGene
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tlr9 - by Bioz Stars, 2024-07
    92/100 stars

    Images

    1) Product Images from "Functional Effects of Toll-Like Receptor (TLR)3, 7, 9, RIG-I and MDA-5 Stimulation in Nasal Epithelial Cells"

    Article Title: Functional Effects of Toll-Like Receptor (TLR)3, 7, 9, RIG-I and MDA-5 Stimulation in Nasal Epithelial Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0098239

    Sections of nasal biopsies were incubated with antibodies against TLR3 ( A ), TLR7 ( B ), TLR9 ( C ), RIG-I ( D ), and MDA-5 ( E ) and visualized by 3, 3′-diaminobenzidine (brown). In control slides ( F ), N-series universal negative control reagent was used. All sections were accompanied with a square magnification. All slides were counterstained with haematoxylin (blue). The figure shows one representative biopsy out of four (3 male, 1 female). The arrows indicate positive stained cells.
    Figure Legend Snippet: Sections of nasal biopsies were incubated with antibodies against TLR3 ( A ), TLR7 ( B ), TLR9 ( C ), RIG-I ( D ), and MDA-5 ( E ) and visualized by 3, 3′-diaminobenzidine (brown). In control slides ( F ), N-series universal negative control reagent was used. All sections were accompanied with a square magnification. All slides were counterstained with haematoxylin (blue). The figure shows one representative biopsy out of four (3 male, 1 female). The arrows indicate positive stained cells.

    Techniques Used: Incubation, Negative Control, Staining

    Epithelial cells from primary HNEC ( A–E ), Detroit-562 ( G–K ) and FaDu ( M–Q ) were incubated with antibodies against TLR3, TLR7, TLR9, RIG-I, and MDA-5 and visualized by 3, 3′-diaminobenzidine (brown). In controls, N-series universal negative control reagent was used ( F, L, R ). All cells were counterstained with haematoxylin (blue). The figure shows one representative staining out of three independent experiments. The markers in the figure are 50 µm.
    Figure Legend Snippet: Epithelial cells from primary HNEC ( A–E ), Detroit-562 ( G–K ) and FaDu ( M–Q ) were incubated with antibodies against TLR3, TLR7, TLR9, RIG-I, and MDA-5 and visualized by 3, 3′-diaminobenzidine (brown). In controls, N-series universal negative control reagent was used ( F, L, R ). All cells were counterstained with haematoxylin (blue). The figure shows one representative staining out of three independent experiments. The markers in the figure are 50 µm.

    Techniques Used: Incubation, Negative Control, Staining

    HNEC ( A ), Detroit-562 ( B ) and FaDu ( C ) were stained intracellularly with Abs against TLR3, TLR7, TLR9, RIG-I and MDA-5 (open histograms) or appropriate isotype control (shaded histograms) and analyzed by flow cytometry. Representative pictures from one out of three independent experiments are shown.
    Figure Legend Snippet: HNEC ( A ), Detroit-562 ( B ) and FaDu ( C ) were stained intracellularly with Abs against TLR3, TLR7, TLR9, RIG-I and MDA-5 (open histograms) or appropriate isotype control (shaded histograms) and analyzed by flow cytometry. Representative pictures from one out of three independent experiments are shown.

    Techniques Used: Staining, Flow Cytometry

    Nasal biopsies and epithelial cells were cultured in the absence (Untreated) or presence of 10 µg/ml poly(I:C) (TLR3), 10 µg/ml R-837 (TLR7), 1 µM CpG (TLR9) and 1 µg/ml poly(I:C)/LyoVec (RIG-I/MDA-5). TNF-α (10 ng/ml) was used as a positive control (data not shown). After 24 h, supernatants from nasal biopsies (n = 5) ( A–D ), HNEC (n = 6–9) ( E–H ), Detroit-562 (n = 5) ( I–L ) and FaDu (n = 5–9) ( M–P ) were collected and analyzed for levels of IL-6, IL-8, GM-CSF and IFN-β using ELISA. Data is presented as mean ± SEM of 5 to 9 independent experiments. *, p<0.05; **, p<0.01; ***, p<0.001.
    Figure Legend Snippet: Nasal biopsies and epithelial cells were cultured in the absence (Untreated) or presence of 10 µg/ml poly(I:C) (TLR3), 10 µg/ml R-837 (TLR7), 1 µM CpG (TLR9) and 1 µg/ml poly(I:C)/LyoVec (RIG-I/MDA-5). TNF-α (10 ng/ml) was used as a positive control (data not shown). After 24 h, supernatants from nasal biopsies (n = 5) ( A–D ), HNEC (n = 6–9) ( E–H ), Detroit-562 (n = 5) ( I–L ) and FaDu (n = 5–9) ( M–P ) were collected and analyzed for levels of IL-6, IL-8, GM-CSF and IFN-β using ELISA. Data is presented as mean ± SEM of 5 to 9 independent experiments. *, p<0.05; **, p<0.01; ***, p<0.001.

    Techniques Used: Cell Culture, Positive Control, Enzyme-linked Immunosorbent Assay

    anti human tlr9 antibody  (Thermo Fisher)


    Bioz Verified Symbol Thermo Fisher is a verified supplier
    Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Thermo Fisher anti human tlr9 antibody
    Toll-like receptor 9 <t>(TLR9)</t> expression in peripheral blood monocytes of late-onset Alzheimer’s disease (LOAD) patients according to TLR9 genotypes. Results are expressed as mean fluorescent intensity (MFI) units by flow cytometry. Statistically significant differences between TLR9 genotypes are indicated.
    Anti Human Tlr9 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human tlr9 antibody/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti human tlr9 antibody - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Toll-like receptor 9 promoter polymorphism is associated with decreased risk of Alzheimer’s disease in Han Chinese"

    Article Title: Toll-like receptor 9 promoter polymorphism is associated with decreased risk of Alzheimer’s disease in Han Chinese

    Journal: Journal of Neuroinflammation

    doi: 10.1186/1742-2094-10-101

    Toll-like receptor 9 (TLR9) expression in peripheral blood monocytes of late-onset Alzheimer’s disease (LOAD) patients according to TLR9 genotypes. Results are expressed as mean fluorescent intensity (MFI) units by flow cytometry. Statistically significant differences between TLR9 genotypes are indicated.
    Figure Legend Snippet: Toll-like receptor 9 (TLR9) expression in peripheral blood monocytes of late-onset Alzheimer’s disease (LOAD) patients according to TLR9 genotypes. Results are expressed as mean fluorescent intensity (MFI) units by flow cytometry. Statistically significant differences between TLR9 genotypes are indicated.

    Techniques Used: Expressing, Flow Cytometry


    Structured Review

    Santa Cruz Biotechnology goat anti human tlr9
    Characterization of primary conjunctival epithelial cells outgrew from VKC explants (VKC-ECs). A : Phase contrast microscopy (Normasky) showing VKC-ECs migrating from explants after 1 week (left, 10× optic field). The picture is representative of all VKC biopsies. A representative forward/side scatter plot of gated primary VKC-ECs (middle) and a FC-CK19 histogram demonstrating the purity of outgrew ECs (right; solid area: background staining (negative control); bold line: 97% CK19 + ECs). B : Confocal analysis specific for TLR4 and <t>TLR9</t> (merge) in VKC-ECs. Control-isotype signal (data not shown) was used in channel series acquisitions, and identical acquisition settings were carried out for all images (60× oil immersion). C : TLR4 / TLR9 transcript expression in untouched primary cultures of VKC-ECs (P 0 ; p<0.01, REST-ANOVA Turkey–Kramer-coupled analysis).
    Goat Anti Human Tlr9, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti human tlr9/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti human tlr9 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Nerve growth factor modulates toll-like receptor (TLR) 4 and 9 expression in cultured primary VKC conjunctival epithelial cells"

    Article Title: Nerve growth factor modulates toll-like receptor (TLR) 4 and 9 expression in cultured primary VKC conjunctival epithelial cells

    Journal: Molecular Vision

    doi:

    Characterization of primary conjunctival epithelial cells outgrew from VKC explants (VKC-ECs). A : Phase contrast microscopy (Normasky) showing VKC-ECs migrating from explants after 1 week (left, 10× optic field). The picture is representative of all VKC biopsies. A representative forward/side scatter plot of gated primary VKC-ECs (middle) and a FC-CK19 histogram demonstrating the purity of outgrew ECs (right; solid area: background staining (negative control); bold line: 97% CK19 + ECs). B : Confocal analysis specific for TLR4 and TLR9 (merge) in VKC-ECs. Control-isotype signal (data not shown) was used in channel series acquisitions, and identical acquisition settings were carried out for all images (60× oil immersion). C : TLR4 / TLR9 transcript expression in untouched primary cultures of VKC-ECs (P 0 ; p<0.01, REST-ANOVA Turkey–Kramer-coupled analysis).
    Figure Legend Snippet: Characterization of primary conjunctival epithelial cells outgrew from VKC explants (VKC-ECs). A : Phase contrast microscopy (Normasky) showing VKC-ECs migrating from explants after 1 week (left, 10× optic field). The picture is representative of all VKC biopsies. A representative forward/side scatter plot of gated primary VKC-ECs (middle) and a FC-CK19 histogram demonstrating the purity of outgrew ECs (right; solid area: background staining (negative control); bold line: 97% CK19 + ECs). B : Confocal analysis specific for TLR4 and TLR9 (merge) in VKC-ECs. Control-isotype signal (data not shown) was used in channel series acquisitions, and identical acquisition settings were carried out for all images (60× oil immersion). C : TLR4 / TLR9 transcript expression in untouched primary cultures of VKC-ECs (P 0 ; p<0.01, REST-ANOVA Turkey–Kramer-coupled analysis).

    Techniques Used: Microscopy, Staining, Negative Control, Expressing

    NGF modulates TLR4/TLR9 expression in VKC conjunctival epithelial cells (VKC-ECs). A : Relative TLR4 / TLR9 transcript expression in few passages VKC-ECs (P 1-2 ). Short-term 1–250 ng/ml NGF exposure induced a steady TLR4 and a slight TLR9 increasing response (p<0.01, REST-ANOVA Tukey–Kramer-coupled analysis). As depicted, the maximum effect was observed at 100 ng/ml NGF, while its specific pretreatment with neutralizing anti-NGF antibody resulted in a downregulation of TLR9 transcripts. Single Ct values were normalized to referring genes, and folds were calculated with respect to their untreated sister cells. B : Confocal analysis specific for TLR4 (middle) and TLR9 (right) in 100 ng/ml NGF-treated VKC-ECs. Control-isotype signal (left) was used in channel series acquisitions, and identical acquisition settings were done for all images (60× oil immersion). C : FACS showed a significant upregulation of both TLR4/TLR9 proteins (5,000 events, right). Note the absence of TLR9 signal in untreated VKC-ECs (left). TLR2 signal was used as internal control since no difference was observed in molecular analysis. Isotype-matched control antibody staining was in 10 1 log decade (IF=7.65; solid area). The graphs are representative of three independent experiments for each sample, which gave identical results.
    Figure Legend Snippet: NGF modulates TLR4/TLR9 expression in VKC conjunctival epithelial cells (VKC-ECs). A : Relative TLR4 / TLR9 transcript expression in few passages VKC-ECs (P 1-2 ). Short-term 1–250 ng/ml NGF exposure induced a steady TLR4 and a slight TLR9 increasing response (p<0.01, REST-ANOVA Tukey–Kramer-coupled analysis). As depicted, the maximum effect was observed at 100 ng/ml NGF, while its specific pretreatment with neutralizing anti-NGF antibody resulted in a downregulation of TLR9 transcripts. Single Ct values were normalized to referring genes, and folds were calculated with respect to their untreated sister cells. B : Confocal analysis specific for TLR4 (middle) and TLR9 (right) in 100 ng/ml NGF-treated VKC-ECs. Control-isotype signal (left) was used in channel series acquisitions, and identical acquisition settings were done for all images (60× oil immersion). C : FACS showed a significant upregulation of both TLR4/TLR9 proteins (5,000 events, right). Note the absence of TLR9 signal in untreated VKC-ECs (left). TLR2 signal was used as internal control since no difference was observed in molecular analysis. Isotype-matched control antibody staining was in 10 1 log decade (IF=7.65; solid area). The graphs are representative of three independent experiments for each sample, which gave identical results.

    Techniques Used: Expressing, Staining

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    R&D Systems tlr9 a405
    Tlr9 A405, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlr9 a405/product/R&D Systems
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tlr9 a405 - by Bioz Stars, 2024-07
    94/100 stars
      Buy from Supplier

    92
    OriGene tlr9
    Sections of nasal biopsies were incubated with antibodies against TLR3 ( A ), TLR7 ( B ), <t>TLR9</t> ( C ), RIG-I ( D ), and MDA-5 ( E ) and visualized by 3, 3′-diaminobenzidine (brown). In control slides ( F ), N-series universal negative control reagent was used. All sections were accompanied with a square magnification. All slides were counterstained with haematoxylin (blue). The figure shows one representative biopsy out of four (3 male, 1 female). The arrows indicate positive stained cells.
    Tlr9, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlr9/product/OriGene
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tlr9 - by Bioz Stars, 2024-07
    92/100 stars
      Buy from Supplier

    86
    Becton Dickinson anti human tlr9 pe
    Sections of nasal biopsies were incubated with antibodies against TLR3 ( A ), TLR7 ( B ), <t>TLR9</t> ( C ), RIG-I ( D ), and MDA-5 ( E ) and visualized by 3, 3′-diaminobenzidine (brown). In control slides ( F ), N-series universal negative control reagent was used. All sections were accompanied with a square magnification. All slides were counterstained with haematoxylin (blue). The figure shows one representative biopsy out of four (3 male, 1 female). The arrows indicate positive stained cells.
    Anti Human Tlr9 Pe, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human tlr9 pe/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti human tlr9 pe - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Thermo Fisher anti human cd3 antibody tlr9 agonist odn2006
    Sections of nasal biopsies were incubated with antibodies against TLR3 ( A ), TLR7 ( B ), <t>TLR9</t> ( C ), RIG-I ( D ), and MDA-5 ( E ) and visualized by 3, 3′-diaminobenzidine (brown). In control slides ( F ), N-series universal negative control reagent was used. All sections were accompanied with a square magnification. All slides were counterstained with haematoxylin (blue). The figure shows one representative biopsy out of four (3 male, 1 female). The arrows indicate positive stained cells.
    Anti Human Cd3 Antibody Tlr9 Agonist Odn2006, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human cd3 antibody tlr9 agonist odn2006/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti human cd3 antibody tlr9 agonist odn2006 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Danaher Inc rabbit polyclonal anti human tlr9 antibody
    Sections of nasal biopsies were incubated with antibodies against TLR3 ( A ), TLR7 ( B ), <t>TLR9</t> ( C ), RIG-I ( D ), and MDA-5 ( E ) and visualized by 3, 3′-diaminobenzidine (brown). In control slides ( F ), N-series universal negative control reagent was used. All sections were accompanied with a square magnification. All slides were counterstained with haematoxylin (blue). The figure shows one representative biopsy out of four (3 male, 1 female). The arrows indicate positive stained cells.
    Rabbit Polyclonal Anti Human Tlr9 Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti human tlr9 antibody/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti human tlr9 antibody - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Thermo Fisher anti human tlr9 pe
    Primers Sequences and the Size of Amplified Fragments for Toll-Like Receptors
    Anti Human Tlr9 Pe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human tlr9 pe/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti human tlr9 pe - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc rabbit anti human tlr9 monoclonal antibody
    Expressions of <t>TLR9</t> in chronic eczema lesions and the perilesional normal skin at the edge (SP *400). ( a ) Epidermic of skin lesion. ( b ) Dermis of skin lesion. ( c ) Perilesional normal skin. ( a ) A few TLR9+ cells with brown cytoplasm were observed in the epidermal of chronic eczema lesions. ( b ) A few TLR9+ cells with brown cytoplasm were observed in the dermal papillary layer of chronic eczema lesion. ( c ) Few TLR9+ cells with brown cytoplasm were observed in both epidermal and papillary layer of perilesional normal skin of the lesion.
    Rabbit Anti Human Tlr9 Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti human tlr9 monoclonal antibody/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti human tlr9 monoclonal antibody - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Abcam rabbit polyclonal anti human tlr9 antibody
    Antibodies and methods selected for the immunohistochemical study
    Rabbit Polyclonal Anti Human Tlr9 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti human tlr9 antibody/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti human tlr9 antibody - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Thermo Fisher anti human tlr9 antibody
    Toll-like receptor 9 <t>(TLR9)</t> expression in peripheral blood monocytes of late-onset Alzheimer’s disease (LOAD) patients according to TLR9 genotypes. Results are expressed as mean fluorescent intensity (MFI) units by flow cytometry. Statistically significant differences between TLR9 genotypes are indicated.
    Anti Human Tlr9 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human tlr9 antibody/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti human tlr9 antibody - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Santa Cruz Biotechnology goat anti human tlr9
    Characterization of primary conjunctival epithelial cells outgrew from VKC explants (VKC-ECs). A : Phase contrast microscopy (Normasky) showing VKC-ECs migrating from explants after 1 week (left, 10× optic field). The picture is representative of all VKC biopsies. A representative forward/side scatter plot of gated primary VKC-ECs (middle) and a FC-CK19 histogram demonstrating the purity of outgrew ECs (right; solid area: background staining (negative control); bold line: 97% CK19 + ECs). B : Confocal analysis specific for TLR4 and <t>TLR9</t> (merge) in VKC-ECs. Control-isotype signal (data not shown) was used in channel series acquisitions, and identical acquisition settings were carried out for all images (60× oil immersion). C : TLR4 / TLR9 transcript expression in untouched primary cultures of VKC-ECs (P 0 ; p<0.01, REST-ANOVA Turkey–Kramer-coupled analysis).
    Goat Anti Human Tlr9, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti human tlr9/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti human tlr9 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    Sections of nasal biopsies were incubated with antibodies against TLR3 ( A ), TLR7 ( B ), TLR9 ( C ), RIG-I ( D ), and MDA-5 ( E ) and visualized by 3, 3′-diaminobenzidine (brown). In control slides ( F ), N-series universal negative control reagent was used. All sections were accompanied with a square magnification. All slides were counterstained with haematoxylin (blue). The figure shows one representative biopsy out of four (3 male, 1 female). The arrows indicate positive stained cells.

    Journal: PLoS ONE

    Article Title: Functional Effects of Toll-Like Receptor (TLR)3, 7, 9, RIG-I and MDA-5 Stimulation in Nasal Epithelial Cells

    doi: 10.1371/journal.pone.0098239

    Figure Lengend Snippet: Sections of nasal biopsies were incubated with antibodies against TLR3 ( A ), TLR7 ( B ), TLR9 ( C ), RIG-I ( D ), and MDA-5 ( E ) and visualized by 3, 3′-diaminobenzidine (brown). In control slides ( F ), N-series universal negative control reagent was used. All sections were accompanied with a square magnification. All slides were counterstained with haematoxylin (blue). The figure shows one representative biopsy out of four (3 male, 1 female). The arrows indicate positive stained cells.

    Article Snippet: The nasal biopsy sections were incubated at 4°C overnight and the epithelial cells at room temperature (RT) for 1 hour with monoclonal mouse anti-human Abs against TLR3 (Cat. no. 40C1285.6; AMS Biotechnology, Abingdon, UK), diluted 1∶20, TLR9 (211-MG-1TLR9; Acris antibodies, Hiddenhausen, Germany), diluted 1∶40 and RIG-I (ab77010; Abcam, Cambridge, UK), diluted 1∶20, or polyclonal rabbit anti-human Abs against TLR7 (ab45371), diluted 1∶20, and MDA-5 (ab69983; Abcam), diluted 1∶40.

    Techniques: Incubation, Negative Control, Staining

    Epithelial cells from primary HNEC ( A–E ), Detroit-562 ( G–K ) and FaDu ( M–Q ) were incubated with antibodies against TLR3, TLR7, TLR9, RIG-I, and MDA-5 and visualized by 3, 3′-diaminobenzidine (brown). In controls, N-series universal negative control reagent was used ( F, L, R ). All cells were counterstained with haematoxylin (blue). The figure shows one representative staining out of three independent experiments. The markers in the figure are 50 µm.

    Journal: PLoS ONE

    Article Title: Functional Effects of Toll-Like Receptor (TLR)3, 7, 9, RIG-I and MDA-5 Stimulation in Nasal Epithelial Cells

    doi: 10.1371/journal.pone.0098239

    Figure Lengend Snippet: Epithelial cells from primary HNEC ( A–E ), Detroit-562 ( G–K ) and FaDu ( M–Q ) were incubated with antibodies against TLR3, TLR7, TLR9, RIG-I, and MDA-5 and visualized by 3, 3′-diaminobenzidine (brown). In controls, N-series universal negative control reagent was used ( F, L, R ). All cells were counterstained with haematoxylin (blue). The figure shows one representative staining out of three independent experiments. The markers in the figure are 50 µm.

    Article Snippet: The nasal biopsy sections were incubated at 4°C overnight and the epithelial cells at room temperature (RT) for 1 hour with monoclonal mouse anti-human Abs against TLR3 (Cat. no. 40C1285.6; AMS Biotechnology, Abingdon, UK), diluted 1∶20, TLR9 (211-MG-1TLR9; Acris antibodies, Hiddenhausen, Germany), diluted 1∶40 and RIG-I (ab77010; Abcam, Cambridge, UK), diluted 1∶20, or polyclonal rabbit anti-human Abs against TLR7 (ab45371), diluted 1∶20, and MDA-5 (ab69983; Abcam), diluted 1∶40.

    Techniques: Incubation, Negative Control, Staining

    HNEC ( A ), Detroit-562 ( B ) and FaDu ( C ) were stained intracellularly with Abs against TLR3, TLR7, TLR9, RIG-I and MDA-5 (open histograms) or appropriate isotype control (shaded histograms) and analyzed by flow cytometry. Representative pictures from one out of three independent experiments are shown.

    Journal: PLoS ONE

    Article Title: Functional Effects of Toll-Like Receptor (TLR)3, 7, 9, RIG-I and MDA-5 Stimulation in Nasal Epithelial Cells

    doi: 10.1371/journal.pone.0098239

    Figure Lengend Snippet: HNEC ( A ), Detroit-562 ( B ) and FaDu ( C ) were stained intracellularly with Abs against TLR3, TLR7, TLR9, RIG-I and MDA-5 (open histograms) or appropriate isotype control (shaded histograms) and analyzed by flow cytometry. Representative pictures from one out of three independent experiments are shown.

    Article Snippet: The nasal biopsy sections were incubated at 4°C overnight and the epithelial cells at room temperature (RT) for 1 hour with monoclonal mouse anti-human Abs against TLR3 (Cat. no. 40C1285.6; AMS Biotechnology, Abingdon, UK), diluted 1∶20, TLR9 (211-MG-1TLR9; Acris antibodies, Hiddenhausen, Germany), diluted 1∶40 and RIG-I (ab77010; Abcam, Cambridge, UK), diluted 1∶20, or polyclonal rabbit anti-human Abs against TLR7 (ab45371), diluted 1∶20, and MDA-5 (ab69983; Abcam), diluted 1∶40.

    Techniques: Staining, Flow Cytometry

    Nasal biopsies and epithelial cells were cultured in the absence (Untreated) or presence of 10 µg/ml poly(I:C) (TLR3), 10 µg/ml R-837 (TLR7), 1 µM CpG (TLR9) and 1 µg/ml poly(I:C)/LyoVec (RIG-I/MDA-5). TNF-α (10 ng/ml) was used as a positive control (data not shown). After 24 h, supernatants from nasal biopsies (n = 5) ( A–D ), HNEC (n = 6–9) ( E–H ), Detroit-562 (n = 5) ( I–L ) and FaDu (n = 5–9) ( M–P ) were collected and analyzed for levels of IL-6, IL-8, GM-CSF and IFN-β using ELISA. Data is presented as mean ± SEM of 5 to 9 independent experiments. *, p<0.05; **, p<0.01; ***, p<0.001.

    Journal: PLoS ONE

    Article Title: Functional Effects of Toll-Like Receptor (TLR)3, 7, 9, RIG-I and MDA-5 Stimulation in Nasal Epithelial Cells

    doi: 10.1371/journal.pone.0098239

    Figure Lengend Snippet: Nasal biopsies and epithelial cells were cultured in the absence (Untreated) or presence of 10 µg/ml poly(I:C) (TLR3), 10 µg/ml R-837 (TLR7), 1 µM CpG (TLR9) and 1 µg/ml poly(I:C)/LyoVec (RIG-I/MDA-5). TNF-α (10 ng/ml) was used as a positive control (data not shown). After 24 h, supernatants from nasal biopsies (n = 5) ( A–D ), HNEC (n = 6–9) ( E–H ), Detroit-562 (n = 5) ( I–L ) and FaDu (n = 5–9) ( M–P ) were collected and analyzed for levels of IL-6, IL-8, GM-CSF and IFN-β using ELISA. Data is presented as mean ± SEM of 5 to 9 independent experiments. *, p<0.05; **, p<0.01; ***, p<0.001.

    Article Snippet: The nasal biopsy sections were incubated at 4°C overnight and the epithelial cells at room temperature (RT) for 1 hour with monoclonal mouse anti-human Abs against TLR3 (Cat. no. 40C1285.6; AMS Biotechnology, Abingdon, UK), diluted 1∶20, TLR9 (211-MG-1TLR9; Acris antibodies, Hiddenhausen, Germany), diluted 1∶40 and RIG-I (ab77010; Abcam, Cambridge, UK), diluted 1∶20, or polyclonal rabbit anti-human Abs against TLR7 (ab45371), diluted 1∶20, and MDA-5 (ab69983; Abcam), diluted 1∶40.

    Techniques: Cell Culture, Positive Control, Enzyme-linked Immunosorbent Assay

    Primers Sequences and the Size of Amplified Fragments for Toll-Like Receptors

    Journal: Journal of Pain Research

    Article Title: T Lymphocyte Subsets Profile and Toll-Like Receptors Responses in Patients with Herpes Zoster

    doi: 10.2147/JPR.S405157

    Figure Lengend Snippet: Primers Sequences and the Size of Amplified Fragments for Toll-Like Receptors

    Article Snippet: The cells were then either intracellularly stained with anti-human TLR2-FITC (No.11–9922-42, eBioscience, USA), anti-human TLR4-PE (No.12–9917-42, eBioscience, USA), anti-human TLR9-PE (No.12–9099-82, eBioscience, USA), or anti-human TLR7-FITC antibody (No.NBP2-25274F, Novus Biologicals, USA) by an intracellular fixation and permeabilization kit (No.88–8824-00, eBioscience, USA) according to the manufacturer’s instructions.

    Techniques: Amplification

    The mRNA expression of toll-like receptors in herpes zoster patients (HZ) and control groups (NC), including TLR2 ( a ), TLR4 ( b ), TLR7 ( c ), and TLR9 ( d ). Data were presented as mean±SD and the significance was analyzed by Student’s t -test, * P <0.05, ** P <0.01.

    Journal: Journal of Pain Research

    Article Title: T Lymphocyte Subsets Profile and Toll-Like Receptors Responses in Patients with Herpes Zoster

    doi: 10.2147/JPR.S405157

    Figure Lengend Snippet: The mRNA expression of toll-like receptors in herpes zoster patients (HZ) and control groups (NC), including TLR2 ( a ), TLR4 ( b ), TLR7 ( c ), and TLR9 ( d ). Data were presented as mean±SD and the significance was analyzed by Student’s t -test, * P <0.05, ** P <0.01.

    Article Snippet: The cells were then either intracellularly stained with anti-human TLR2-FITC (No.11–9922-42, eBioscience, USA), anti-human TLR4-PE (No.12–9917-42, eBioscience, USA), anti-human TLR9-PE (No.12–9099-82, eBioscience, USA), or anti-human TLR7-FITC antibody (No.NBP2-25274F, Novus Biologicals, USA) by an intracellular fixation and permeabilization kit (No.88–8824-00, eBioscience, USA) according to the manufacturer’s instructions.

    Techniques: Expressing

    Expressions of TLR9 in chronic eczema lesions and the perilesional normal skin at the edge (SP *400). ( a ) Epidermic of skin lesion. ( b ) Dermis of skin lesion. ( c ) Perilesional normal skin. ( a ) A few TLR9+ cells with brown cytoplasm were observed in the epidermal of chronic eczema lesions. ( b ) A few TLR9+ cells with brown cytoplasm were observed in the dermal papillary layer of chronic eczema lesion. ( c ) Few TLR9+ cells with brown cytoplasm were observed in both epidermal and papillary layer of perilesional normal skin of the lesion.

    Journal: Clinical, Cosmetic and Investigational Dermatology

    Article Title: The Expression of Plasmacytoid Dendritic Cells and TLR7/9-MyD88-IRAKs Pathway in Chronic Eczema Lesions

    doi: 10.2147/CCID.S405491

    Figure Lengend Snippet: Expressions of TLR9 in chronic eczema lesions and the perilesional normal skin at the edge (SP *400). ( a ) Epidermic of skin lesion. ( b ) Dermis of skin lesion. ( c ) Perilesional normal skin. ( a ) A few TLR9+ cells with brown cytoplasm were observed in the epidermal of chronic eczema lesions. ( b ) A few TLR9+ cells with brown cytoplasm were observed in the dermal papillary layer of chronic eczema lesion. ( c ) Few TLR9+ cells with brown cytoplasm were observed in both epidermal and papillary layer of perilesional normal skin of the lesion.

    Article Snippet: Rabbit anti-human CD2AP monoclonal antibody and mouse anti-human CD123 monoclonal antibody were bought from Abcam, USA, and rabbit anti-human IRAK1 monoclonal antibody, rabbit anti-human IRF7 monoclonal antibody, mouse anti-human TLR7 monoclonal antibody, and rabbit anti-human TLR9 monoclonal antibody were bought from CST, USA.

    Techniques:

    The Positive Rate and Intensity of  TLR9  Expression in 16 Cases of Chronic Eczema and 14 Cases of Normal Skin Around Eczema

    Journal: Clinical, Cosmetic and Investigational Dermatology

    Article Title: The Expression of Plasmacytoid Dendritic Cells and TLR7/9-MyD88-IRAKs Pathway in Chronic Eczema Lesions

    doi: 10.2147/CCID.S405491

    Figure Lengend Snippet: The Positive Rate and Intensity of TLR9 Expression in 16 Cases of Chronic Eczema and 14 Cases of Normal Skin Around Eczema

    Article Snippet: Rabbit anti-human CD2AP monoclonal antibody and mouse anti-human CD123 monoclonal antibody were bought from Abcam, USA, and rabbit anti-human IRAK1 monoclonal antibody, rabbit anti-human IRF7 monoclonal antibody, mouse anti-human TLR7 monoclonal antibody, and rabbit anti-human TLR9 monoclonal antibody were bought from CST, USA.

    Techniques: Expressing

    Antibodies and methods selected for the immunohistochemical study

    Journal: Romanian Journal of Morphology and Embryology

    Article Title: Involvement of TLR9 in priming the immune response in oral papillomatosis induced by low-risk HPV

    doi: 10.47162/RJME.64.2.08

    Figure Lengend Snippet: Antibodies and methods selected for the immunohistochemical study

    Article Snippet: Rabbit polyclonal anti-human TLR9 antibody , 1:100 , Abcam anti-TLR9 antibody-N-terminal (Ab227004) , EDTA buffer, pH 7 , ImmPRESS ® HRP goat anti-rabbit IgG (Vector Laboratories, Inc.).

    Techniques: Immunohistochemistry, Amplification, Plasmid Preparation

    HPV-negative oral papillomatosis: (a–d) Immunoreaction for TLR9; (e–h) Immunoreaction for CD68. (a–d, g, and h) ×200; (f) ×100. CD68: Cluster of differentiation 68; TLR9: Toll-like receptor 9.

    Journal: Romanian Journal of Morphology and Embryology

    Article Title: Involvement of TLR9 in priming the immune response in oral papillomatosis induced by low-risk HPV

    doi: 10.47162/RJME.64.2.08

    Figure Lengend Snippet: HPV-negative oral papillomatosis: (a–d) Immunoreaction for TLR9; (e–h) Immunoreaction for CD68. (a–d, g, and h) ×200; (f) ×100. CD68: Cluster of differentiation 68; TLR9: Toll-like receptor 9.

    Article Snippet: Rabbit polyclonal anti-human TLR9 antibody , 1:100 , Abcam anti-TLR9 antibody-N-terminal (Ab227004) , EDTA buffer, pH 7 , ImmPRESS ® HRP goat anti-rabbit IgG (Vector Laboratories, Inc.).

    Techniques:

    Low-risk HPV-positive oral papillomatosis: (a–d) Immunoreaction for TLR9; (e–h) Immunoreaction for CD68. (a–g) ×200; (h) ×45.

    Journal: Romanian Journal of Morphology and Embryology

    Article Title: Involvement of TLR9 in priming the immune response in oral papillomatosis induced by low-risk HPV

    doi: 10.47162/RJME.64.2.08

    Figure Lengend Snippet: Low-risk HPV-positive oral papillomatosis: (a–d) Immunoreaction for TLR9; (e–h) Immunoreaction for CD68. (a–g) ×200; (h) ×45.

    Article Snippet: Rabbit polyclonal anti-human TLR9 antibody , 1:100 , Abcam anti-TLR9 antibody-N-terminal (Ab227004) , EDTA buffer, pH 7 , ImmPRESS ® HRP goat anti-rabbit IgG (Vector Laboratories, Inc.).

    Techniques:

    Spearman’s correlation between the macrophage number and other variables of  TLR9  immunohistochemical signal

    Journal: Romanian Journal of Morphology and Embryology

    Article Title: Involvement of TLR9 in priming the immune response in oral papillomatosis induced by low-risk HPV

    doi: 10.47162/RJME.64.2.08

    Figure Lengend Snippet: Spearman’s correlation between the macrophage number and other variables of TLR9 immunohistochemical signal

    Article Snippet: Rabbit polyclonal anti-human TLR9 antibody , 1:100 , Abcam anti-TLR9 antibody-N-terminal (Ab227004) , EDTA buffer, pH 7 , ImmPRESS ® HRP goat anti-rabbit IgG (Vector Laboratories, Inc.).

    Techniques: Immunohistochemistry

    Toll-like receptor 9 (TLR9) expression in peripheral blood monocytes of late-onset Alzheimer’s disease (LOAD) patients according to TLR9 genotypes. Results are expressed as mean fluorescent intensity (MFI) units by flow cytometry. Statistically significant differences between TLR9 genotypes are indicated.

    Journal: Journal of Neuroinflammation

    Article Title: Toll-like receptor 9 promoter polymorphism is associated with decreased risk of Alzheimer’s disease in Han Chinese

    doi: 10.1186/1742-2094-10-101

    Figure Lengend Snippet: Toll-like receptor 9 (TLR9) expression in peripheral blood monocytes of late-onset Alzheimer’s disease (LOAD) patients according to TLR9 genotypes. Results are expressed as mean fluorescent intensity (MFI) units by flow cytometry. Statistically significant differences between TLR9 genotypes are indicated.

    Article Snippet: After being washed, the cells were permeabilized, and then stained with anti-human TLR9 antibody (eBioscience, San Diego, CA, USA).

    Techniques: Expressing, Flow Cytometry

    Characterization of primary conjunctival epithelial cells outgrew from VKC explants (VKC-ECs). A : Phase contrast microscopy (Normasky) showing VKC-ECs migrating from explants after 1 week (left, 10× optic field). The picture is representative of all VKC biopsies. A representative forward/side scatter plot of gated primary VKC-ECs (middle) and a FC-CK19 histogram demonstrating the purity of outgrew ECs (right; solid area: background staining (negative control); bold line: 97% CK19 + ECs). B : Confocal analysis specific for TLR4 and TLR9 (merge) in VKC-ECs. Control-isotype signal (data not shown) was used in channel series acquisitions, and identical acquisition settings were carried out for all images (60× oil immersion). C : TLR4 / TLR9 transcript expression in untouched primary cultures of VKC-ECs (P 0 ; p<0.01, REST-ANOVA Turkey–Kramer-coupled analysis).

    Journal: Molecular Vision

    Article Title: Nerve growth factor modulates toll-like receptor (TLR) 4 and 9 expression in cultured primary VKC conjunctival epithelial cells

    doi:

    Figure Lengend Snippet: Characterization of primary conjunctival epithelial cells outgrew from VKC explants (VKC-ECs). A : Phase contrast microscopy (Normasky) showing VKC-ECs migrating from explants after 1 week (left, 10× optic field). The picture is representative of all VKC biopsies. A representative forward/side scatter plot of gated primary VKC-ECs (middle) and a FC-CK19 histogram demonstrating the purity of outgrew ECs (right; solid area: background staining (negative control); bold line: 97% CK19 + ECs). B : Confocal analysis specific for TLR4 and TLR9 (merge) in VKC-ECs. Control-isotype signal (data not shown) was used in channel series acquisitions, and identical acquisition settings were carried out for all images (60× oil immersion). C : TLR4 / TLR9 transcript expression in untouched primary cultures of VKC-ECs (P 0 ; p<0.01, REST-ANOVA Turkey–Kramer-coupled analysis).

    Article Snippet: A brief blocking/permeabilizing step (0.8% bovine serum albumin [BSA] and 0.3% Triton-X100 in PBS) was performed before the addition of the specific antibodies: rabbit anti-human TLR4 (sc-10741; Santa Cruz Biotech, Santa Cruz, CA), goat anti-human TLR9 (sc-16247; Santa Cruz Biotech), mouse anti-human trkA NGFR and goat anti-human p75 NTR antibodies (0.5–1 µg/ml; R&D).

    Techniques: Microscopy, Staining, Negative Control, Expressing

    NGF modulates TLR4/TLR9 expression in VKC conjunctival epithelial cells (VKC-ECs). A : Relative TLR4 / TLR9 transcript expression in few passages VKC-ECs (P 1-2 ). Short-term 1–250 ng/ml NGF exposure induced a steady TLR4 and a slight TLR9 increasing response (p<0.01, REST-ANOVA Tukey–Kramer-coupled analysis). As depicted, the maximum effect was observed at 100 ng/ml NGF, while its specific pretreatment with neutralizing anti-NGF antibody resulted in a downregulation of TLR9 transcripts. Single Ct values were normalized to referring genes, and folds were calculated with respect to their untreated sister cells. B : Confocal analysis specific for TLR4 (middle) and TLR9 (right) in 100 ng/ml NGF-treated VKC-ECs. Control-isotype signal (left) was used in channel series acquisitions, and identical acquisition settings were done for all images (60× oil immersion). C : FACS showed a significant upregulation of both TLR4/TLR9 proteins (5,000 events, right). Note the absence of TLR9 signal in untreated VKC-ECs (left). TLR2 signal was used as internal control since no difference was observed in molecular analysis. Isotype-matched control antibody staining was in 10 1 log decade (IF=7.65; solid area). The graphs are representative of three independent experiments for each sample, which gave identical results.

    Journal: Molecular Vision

    Article Title: Nerve growth factor modulates toll-like receptor (TLR) 4 and 9 expression in cultured primary VKC conjunctival epithelial cells

    doi:

    Figure Lengend Snippet: NGF modulates TLR4/TLR9 expression in VKC conjunctival epithelial cells (VKC-ECs). A : Relative TLR4 / TLR9 transcript expression in few passages VKC-ECs (P 1-2 ). Short-term 1–250 ng/ml NGF exposure induced a steady TLR4 and a slight TLR9 increasing response (p<0.01, REST-ANOVA Tukey–Kramer-coupled analysis). As depicted, the maximum effect was observed at 100 ng/ml NGF, while its specific pretreatment with neutralizing anti-NGF antibody resulted in a downregulation of TLR9 transcripts. Single Ct values were normalized to referring genes, and folds were calculated with respect to their untreated sister cells. B : Confocal analysis specific for TLR4 (middle) and TLR9 (right) in 100 ng/ml NGF-treated VKC-ECs. Control-isotype signal (left) was used in channel series acquisitions, and identical acquisition settings were done for all images (60× oil immersion). C : FACS showed a significant upregulation of both TLR4/TLR9 proteins (5,000 events, right). Note the absence of TLR9 signal in untreated VKC-ECs (left). TLR2 signal was used as internal control since no difference was observed in molecular analysis. Isotype-matched control antibody staining was in 10 1 log decade (IF=7.65; solid area). The graphs are representative of three independent experiments for each sample, which gave identical results.

    Article Snippet: A brief blocking/permeabilizing step (0.8% bovine serum albumin [BSA] and 0.3% Triton-X100 in PBS) was performed before the addition of the specific antibodies: rabbit anti-human TLR4 (sc-10741; Santa Cruz Biotech, Santa Cruz, CA), goat anti-human TLR9 (sc-16247; Santa Cruz Biotech), mouse anti-human trkA NGFR and goat anti-human p75 NTR antibodies (0.5–1 µg/ml; R&D).

    Techniques: Expressing, Staining