anti human tlr4 monoclonal mouse igg1  (InvivoGen)

Journal: Scientific Reports

Article Title: TLR4 antagonist FP7 inhibits LPS-induced cytokine production and glycolytic reprogramming in dendritic cells, and protects mice from lethal influenza infection

doi: 10.1038/srep40791

Figure Lengend Snippet: DCs were seeded at 1 × 10 6 cells/ml in fresh medium and stimulated for 24 h with increasing amounts of LPS, in the absence or presence of 10 μM FP7 ( a , b ). Control cells (Ctl) received solvent instead of FP7. Glucose consumption and lactate production were monitored using enzymatic detection kits. Means ± SEM from 5 or 6 experiments are shown. ( c ) DCs were incubated with 10 μg/ml TLR4-blocking antibody or isotype control antibody 1 h prior to LPS addition. Glucose consumption was normalized to non-stimulated control DCs and means ± SEM from 3 experiments are shown. *P < 0.05; # P < 0.01 FP7 compared to Ctl cells.

Article Snippet: Neutralizing mouse IgG monoclonal anti-human TLR4 and non-blocking control antibodies were kindly provided by Dr. F. Neumann, Innaxon Biosciences.

Techniques: Incubation, Blocking Assay