Review



anti human tlr4 igg2a kappa anti tlr4  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Thermo Fisher anti human tlr4 igg2a kappa anti tlr4
    SARS‐CoV‐2 envelope protein, but not spike protein, induces the secretion of cytokines from human alveolar macrophages. AMs were isolated from BAL fluid of patients undergoing routine bronchoscopy by adherence purification. AMs were pre‐treated with an isotype control antibody (control <t>IgG)</t> (10 μg/mL) or untreated for 1 h and then stimulated with SARS‐CoV‐2 spike protein or envelope protein (both 1 μg/mL). Untreated AMs that were stimulated with LPS (100 ng/mL) or unstimulated were used as positive and negative controls, respectively. Supernatant was collected at 24 h poststimulation. The concentration of cytokines was measured using an MSD multiplex assay ( n = 9). Statistical comparisons were made using the Friedman test with Dunn's multiple comparisons test.
    Anti Human Tlr4 Igg2a Kappa Anti Tlr4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human tlr4 igg2a kappa anti tlr4/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    anti human tlr4 igg2a kappa anti tlr4 - by Bioz Stars, 2025-07
    86/100 stars

    Images

    1) Product Images from "Human Alveolar Macrophages Detect SARS‐CoV‐2 Envelope Protein Through TLR2 and TLR4 and Secrete Cytokines in Response"

    Article Title: Human Alveolar Macrophages Detect SARS‐CoV‐2 Envelope Protein Through TLR2 and TLR4 and Secrete Cytokines in Response

    Journal: Immunology

    doi: 10.1111/imm.13922

    SARS‐CoV‐2 envelope protein, but not spike protein, induces the secretion of cytokines from human alveolar macrophages. AMs were isolated from BAL fluid of patients undergoing routine bronchoscopy by adherence purification. AMs were pre‐treated with an isotype control antibody (control IgG) (10 μg/mL) or untreated for 1 h and then stimulated with SARS‐CoV‐2 spike protein or envelope protein (both 1 μg/mL). Untreated AMs that were stimulated with LPS (100 ng/mL) or unstimulated were used as positive and negative controls, respectively. Supernatant was collected at 24 h poststimulation. The concentration of cytokines was measured using an MSD multiplex assay ( n = 9). Statistical comparisons were made using the Friedman test with Dunn's multiple comparisons test.
    Figure Legend Snippet: SARS‐CoV‐2 envelope protein, but not spike protein, induces the secretion of cytokines from human alveolar macrophages. AMs were isolated from BAL fluid of patients undergoing routine bronchoscopy by adherence purification. AMs were pre‐treated with an isotype control antibody (control IgG) (10 μg/mL) or untreated for 1 h and then stimulated with SARS‐CoV‐2 spike protein or envelope protein (both 1 μg/mL). Untreated AMs that were stimulated with LPS (100 ng/mL) or unstimulated were used as positive and negative controls, respectively. Supernatant was collected at 24 h poststimulation. The concentration of cytokines was measured using an MSD multiplex assay ( n = 9). Statistical comparisons were made using the Friedman test with Dunn's multiple comparisons test.

    Techniques Used: Isolation, Purification, Control, Concentration Assay, Multiplex Assay

    Blocking antibodies against TLR2 and TLR4 alter the cytokine responses of human alveolar macrophages to SARS‐CoV‐2 envelope protein. AMs were isolated and treated with blocking antibodies against TLR2, TLR4, or an isotype control antibody (control IgG) (all 10 μg/mL) and stimulated as previously described. Supernatant was collected at 24‐h post stimulation. The concentration of cytokines was measured using an MSD multiplex assay ( n = 9). Statistical comparisons were made using the Friedman test with Dunn's multiple comparisons test.
    Figure Legend Snippet: Blocking antibodies against TLR2 and TLR4 alter the cytokine responses of human alveolar macrophages to SARS‐CoV‐2 envelope protein. AMs were isolated and treated with blocking antibodies against TLR2, TLR4, or an isotype control antibody (control IgG) (all 10 μg/mL) and stimulated as previously described. Supernatant was collected at 24‐h post stimulation. The concentration of cytokines was measured using an MSD multiplex assay ( n = 9). Statistical comparisons were made using the Friedman test with Dunn's multiple comparisons test.

    Techniques Used: Blocking Assay, Isolation, Control, Concentration Assay, Multiplex Assay

    Alveolar macrophages from donors aged over 70 years had higher cytokine responses to SARS‐CoV‐2 envelope protein than younger donors. AMs were isolated, treated,and stimulated as previously described. Supernatant was collected at 24 h poststimulation. The concentration of cytokines was measured using an MSD multiplex assay. The statistical comparison presented here is younger ( n = 5) and older ( n = 4) donor AMs treated with control IgG, then stimulated with envelope protein, or unstimulated. Planned statistical comparisons were made between stimulated cells from donors aged < 70 years versus those from donors aged > 70 years using two‐way ANOVA with Šídák's multiple comparison test.
    Figure Legend Snippet: Alveolar macrophages from donors aged over 70 years had higher cytokine responses to SARS‐CoV‐2 envelope protein than younger donors. AMs were isolated, treated,and stimulated as previously described. Supernatant was collected at 24 h poststimulation. The concentration of cytokines was measured using an MSD multiplex assay. The statistical comparison presented here is younger ( n = 5) and older ( n = 4) donor AMs treated with control IgG, then stimulated with envelope protein, or unstimulated. Planned statistical comparisons were made between stimulated cells from donors aged < 70 years versus those from donors aged > 70 years using two‐way ANOVA with Šídák's multiple comparison test.

    Techniques Used: Isolation, Concentration Assay, Multiplex Assay, Comparison, Control

    Alveolar macrophages from smokers had lower cytokine responses to SARS‐CoV‐2 envelope protein than non‐smokers. AMs were isolated, treated, and stimulated as previously described. Supernatant was collected at 24‐h poststimulation. The concentration of cytokines was measured using an MSD multiplex assay. The statistical comparison presented here is smokers' ( n = 5) and non‐smokers' ( n = 4) AMs treated with control IgG, then stimulated with envelope protein, or unstimulated. Planned statistical comparisons were made between stimulated cells from never/ex‐smokers versus those from current smokers using two‐way ANOVA with Šídák's multiple comparison test.
    Figure Legend Snippet: Alveolar macrophages from smokers had lower cytokine responses to SARS‐CoV‐2 envelope protein than non‐smokers. AMs were isolated, treated, and stimulated as previously described. Supernatant was collected at 24‐h poststimulation. The concentration of cytokines was measured using an MSD multiplex assay. The statistical comparison presented here is smokers' ( n = 5) and non‐smokers' ( n = 4) AMs treated with control IgG, then stimulated with envelope protein, or unstimulated. Planned statistical comparisons were made between stimulated cells from never/ex‐smokers versus those from current smokers using two‐way ANOVA with Šídák's multiple comparison test.

    Techniques Used: Isolation, Concentration Assay, Multiplex Assay, Comparison, Control



    Similar Products

    anti human tlr4 igg2a kappa anti tlr4  (Thermo Fisher)
    86
    Thermo Fisher anti human tlr4 igg2a kappa anti tlr4
    SARS‐CoV‐2 envelope protein, but not spike protein, induces the secretion of cytokines from human alveolar macrophages. AMs were isolated from BAL fluid of patients undergoing routine bronchoscopy by adherence purification. AMs were pre‐treated with an isotype control antibody (control <t>IgG)</t> (10 μg/mL) or untreated for 1 h and then stimulated with SARS‐CoV‐2 spike protein or envelope protein (both 1 μg/mL). Untreated AMs that were stimulated with LPS (100 ng/mL) or unstimulated were used as positive and negative controls, respectively. Supernatant was collected at 24 h poststimulation. The concentration of cytokines was measured using an MSD multiplex assay ( n = 9). Statistical comparisons were made using the Friedman test with Dunn's multiple comparisons test.
    Anti Human Tlr4 Igg2a Kappa Anti Tlr4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human tlr4 igg2a kappa anti tlr4/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    anti human tlr4 igg2a kappa anti tlr4 - by Bioz Stars, 2025-07
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    SARS‐CoV‐2 envelope protein, but not spike protein, induces the secretion of cytokines from human alveolar macrophages. AMs were isolated from BAL fluid of patients undergoing routine bronchoscopy by adherence purification. AMs were pre‐treated with an isotype control antibody (control IgG) (10 μg/mL) or untreated for 1 h and then stimulated with SARS‐CoV‐2 spike protein or envelope protein (both 1 μg/mL). Untreated AMs that were stimulated with LPS (100 ng/mL) or unstimulated were used as positive and negative controls, respectively. Supernatant was collected at 24 h poststimulation. The concentration of cytokines was measured using an MSD multiplex assay ( n = 9). Statistical comparisons were made using the Friedman test with Dunn's multiple comparisons test.

    Journal: Immunology

    Article Title: Human Alveolar Macrophages Detect SARS‐CoV‐2 Envelope Protein Through TLR2 and TLR4 and Secrete Cytokines in Response

    doi: 10.1111/imm.13922

    Figure Lengend Snippet: SARS‐CoV‐2 envelope protein, but not spike protein, induces the secretion of cytokines from human alveolar macrophages. AMs were isolated from BAL fluid of patients undergoing routine bronchoscopy by adherence purification. AMs were pre‐treated with an isotype control antibody (control IgG) (10 μg/mL) or untreated for 1 h and then stimulated with SARS‐CoV‐2 spike protein or envelope protein (both 1 μg/mL). Untreated AMs that were stimulated with LPS (100 ng/mL) or unstimulated were used as positive and negative controls, respectively. Supernatant was collected at 24 h poststimulation. The concentration of cytokines was measured using an MSD multiplex assay ( n = 9). Statistical comparisons were made using the Friedman test with Dunn's multiple comparisons test.

    Article Snippet: Monoclonal anti‐human TLR2 IgG2a kappa (anti‐TLR2) (ThermoFisher; 16‐9922), monoclonal anti‐human TLR4 IgG2a kappa (anti‐TLR4) (ThermoFisher; 16‐9917) and isotype control anti‐mouse IgG2a kappa (ThermoFisher; 16‐4724) were reconstituted in sterile PBS and stored at 4°C prior to experimentation.

    Techniques: Isolation, Purification, Control, Concentration Assay, Multiplex Assay

    Blocking antibodies against TLR2 and TLR4 alter the cytokine responses of human alveolar macrophages to SARS‐CoV‐2 envelope protein. AMs were isolated and treated with blocking antibodies against TLR2, TLR4, or an isotype control antibody (control IgG) (all 10 μg/mL) and stimulated as previously described. Supernatant was collected at 24‐h post stimulation. The concentration of cytokines was measured using an MSD multiplex assay ( n = 9). Statistical comparisons were made using the Friedman test with Dunn's multiple comparisons test.

    Journal: Immunology

    Article Title: Human Alveolar Macrophages Detect SARS‐CoV‐2 Envelope Protein Through TLR2 and TLR4 and Secrete Cytokines in Response

    doi: 10.1111/imm.13922

    Figure Lengend Snippet: Blocking antibodies against TLR2 and TLR4 alter the cytokine responses of human alveolar macrophages to SARS‐CoV‐2 envelope protein. AMs were isolated and treated with blocking antibodies against TLR2, TLR4, or an isotype control antibody (control IgG) (all 10 μg/mL) and stimulated as previously described. Supernatant was collected at 24‐h post stimulation. The concentration of cytokines was measured using an MSD multiplex assay ( n = 9). Statistical comparisons were made using the Friedman test with Dunn's multiple comparisons test.

    Article Snippet: Monoclonal anti‐human TLR2 IgG2a kappa (anti‐TLR2) (ThermoFisher; 16‐9922), monoclonal anti‐human TLR4 IgG2a kappa (anti‐TLR4) (ThermoFisher; 16‐9917) and isotype control anti‐mouse IgG2a kappa (ThermoFisher; 16‐4724) were reconstituted in sterile PBS and stored at 4°C prior to experimentation.

    Techniques: Blocking Assay, Isolation, Control, Concentration Assay, Multiplex Assay

    Alveolar macrophages from donors aged over 70 years had higher cytokine responses to SARS‐CoV‐2 envelope protein than younger donors. AMs were isolated, treated,and stimulated as previously described. Supernatant was collected at 24 h poststimulation. The concentration of cytokines was measured using an MSD multiplex assay. The statistical comparison presented here is younger ( n = 5) and older ( n = 4) donor AMs treated with control IgG, then stimulated with envelope protein, or unstimulated. Planned statistical comparisons were made between stimulated cells from donors aged < 70 years versus those from donors aged > 70 years using two‐way ANOVA with Šídák's multiple comparison test.

    Journal: Immunology

    Article Title: Human Alveolar Macrophages Detect SARS‐CoV‐2 Envelope Protein Through TLR2 and TLR4 and Secrete Cytokines in Response

    doi: 10.1111/imm.13922

    Figure Lengend Snippet: Alveolar macrophages from donors aged over 70 years had higher cytokine responses to SARS‐CoV‐2 envelope protein than younger donors. AMs were isolated, treated,and stimulated as previously described. Supernatant was collected at 24 h poststimulation. The concentration of cytokines was measured using an MSD multiplex assay. The statistical comparison presented here is younger ( n = 5) and older ( n = 4) donor AMs treated with control IgG, then stimulated with envelope protein, or unstimulated. Planned statistical comparisons were made between stimulated cells from donors aged < 70 years versus those from donors aged > 70 years using two‐way ANOVA with Šídák's multiple comparison test.

    Article Snippet: Monoclonal anti‐human TLR2 IgG2a kappa (anti‐TLR2) (ThermoFisher; 16‐9922), monoclonal anti‐human TLR4 IgG2a kappa (anti‐TLR4) (ThermoFisher; 16‐9917) and isotype control anti‐mouse IgG2a kappa (ThermoFisher; 16‐4724) were reconstituted in sterile PBS and stored at 4°C prior to experimentation.

    Techniques: Isolation, Concentration Assay, Multiplex Assay, Comparison, Control

    Alveolar macrophages from smokers had lower cytokine responses to SARS‐CoV‐2 envelope protein than non‐smokers. AMs were isolated, treated, and stimulated as previously described. Supernatant was collected at 24‐h poststimulation. The concentration of cytokines was measured using an MSD multiplex assay. The statistical comparison presented here is smokers' ( n = 5) and non‐smokers' ( n = 4) AMs treated with control IgG, then stimulated with envelope protein, or unstimulated. Planned statistical comparisons were made between stimulated cells from never/ex‐smokers versus those from current smokers using two‐way ANOVA with Šídák's multiple comparison test.

    Journal: Immunology

    Article Title: Human Alveolar Macrophages Detect SARS‐CoV‐2 Envelope Protein Through TLR2 and TLR4 and Secrete Cytokines in Response

    doi: 10.1111/imm.13922

    Figure Lengend Snippet: Alveolar macrophages from smokers had lower cytokine responses to SARS‐CoV‐2 envelope protein than non‐smokers. AMs were isolated, treated, and stimulated as previously described. Supernatant was collected at 24‐h poststimulation. The concentration of cytokines was measured using an MSD multiplex assay. The statistical comparison presented here is smokers' ( n = 5) and non‐smokers' ( n = 4) AMs treated with control IgG, then stimulated with envelope protein, or unstimulated. Planned statistical comparisons were made between stimulated cells from never/ex‐smokers versus those from current smokers using two‐way ANOVA with Šídák's multiple comparison test.

    Article Snippet: Monoclonal anti‐human TLR2 IgG2a kappa (anti‐TLR2) (ThermoFisher; 16‐9922), monoclonal anti‐human TLR4 IgG2a kappa (anti‐TLR4) (ThermoFisher; 16‐9917) and isotype control anti‐mouse IgG2a kappa (ThermoFisher; 16‐4724) were reconstituted in sterile PBS and stored at 4°C prior to experimentation.

    Techniques: Isolation, Concentration Assay, Multiplex Assay, Comparison, Control