anti human tlr 4 antibody  (Thermo Fisher)

Journal: PLoS ONE

Article Title: Biologically Active Polymers from Spontaneous Carotenoid Oxidation: A New Frontier in Carotenoid Activity

doi: 10.1371/journal.pone.0111346

Figure Lengend Snippet: Balb/c mice were not supplemented (control) or supplemented daily by oral gavage with OxC-beta (10 mg/kg). After 4 weeks, intestinal tissues were harvested and CD14 and TLR-4 expression was determined by immunocytochemistry. Increased CD14 (A) and TLR-4 (C) expression is readily apparent in epithelial cells in the OxC-beta-supplemented animals compared to the controls receiving vehicle alone (B and D, respectively). Arrows indicate the location of enterocytes within the cross section of microvilli. Magnification 40x.

Article Snippet: Cells were stained for CD14, TLR-4, or TLR-2 by incubation for 1 hour at 4°C with the following phycoerythrin (PE) conjugated antibodies: anti-human CD14 (eBioscience 12–0149), anti-human TLR-4 (eBioscience 12–9917), and anti-human TLR-2 (eBioscience 12–9922).

Techniques: Expressing, Immunocytochemistry