anti human sma igg  (Abcam)

 
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    Name:
    Goat Anti Human IgG Fab 2 Alkaline Phosphatase
    Description:

    Catalog Number:
    AB87408
    Price:
    None
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    Structured Review

    Abcam anti human sma igg

    https://www.bioz.com/result/anti human sma igg/product/Abcam
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti human sma igg - by Bioz Stars, 2021-07
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: ATORVASTATIN OR TRANSGENIC EXPRESSION OF TFPI INHIBITS COAGULATION INITIATED BY ANTI-NONGAL IgG BINDING TO PORCINE AORTIC ENDOTHELIAL CELLS
    Article Snippet: .. To investigate further, HI sensitized baboon serum was pretreated with an anti-human IgG Fab Ab for 30min at 4°C before incubation with PAECs. .. The anti-human IgG Fab Ab blocked the effect of HI sensitized baboon serum to induce WT PAEC TF activity ( ).

    Article Title: Cell Type-Specific Expression and Function of Toll-Like Receptors 2 and 4 in Human Placenta: Implications in Fetal Infection
    Article Snippet: Proteins were then transferred to Immobilon paper (Millipore Corp, Bedford, MA) at 100 volts for 80 min. .. Following transfer, blots were incubated with primary antibodies (the sources are described above in immunohistochemical studies) at the following dilutions: goat anti-human TLR-2 (1:1000); TLR-4 (1:500); mouse anti-human αSMA (1:15,000); and rabbit anti-human cyclophilin B (Abcam, Cambridge, MA, 1:2000). .. Overnight incubation with primary antibody at 4º C was followed by a 1 h incubation with rabbit anti-goat (1:2000), goat anti-mouse (1:15,000), or sheep anti-rabbit (1:5,000) horseradish secondary antibody conjugates (Bio-Rad, Hercules, CA).

    Article Title: Serum immunoglobulin G antibody titer to Fusobacterium nucleatum is associated with unfavorable outcome after stroke
    Article Snippet: .. After incubation at 4°C overnight, the wells were washed with PBST, then filled with alkaline phosphatase‐conjugated goat anti‐human IgG (gamma‐chain specific; Abcam, Cambridge, MA, USA) in PBST. .. After another incubation at 37°C for 2 h, the wells were again washed with PBST, then an aliquot of p‐nitrophenylphosphate at 1 mg/m; (Wako Pure Chemical Industries Ltd., Osaka, Japan) in 10% diethanolamine buffer was added to each well as a substrate and incubation was performed at 37°C for 30 min. Optical density at 405 nm was measured using a microplate reader (iMark; Bio‐Rad Laboratories Inc., Hercules, CA, USA).

    Immunohistochemistry:

    Article Title: Cell Type-Specific Expression and Function of Toll-Like Receptors 2 and 4 in Human Placenta: Implications in Fetal Infection
    Article Snippet: Proteins were then transferred to Immobilon paper (Millipore Corp, Bedford, MA) at 100 volts for 80 min. .. Following transfer, blots were incubated with primary antibodies (the sources are described above in immunohistochemical studies) at the following dilutions: goat anti-human TLR-2 (1:1000); TLR-4 (1:500); mouse anti-human αSMA (1:15,000); and rabbit anti-human cyclophilin B (Abcam, Cambridge, MA, 1:2000). .. Overnight incubation with primary antibody at 4º C was followed by a 1 h incubation with rabbit anti-goat (1:2000), goat anti-mouse (1:15,000), or sheep anti-rabbit (1:5,000) horseradish secondary antibody conjugates (Bio-Rad, Hercules, CA).

    Activity Assay:

    Article Title: ATORVASTATIN OR TRANSGENIC EXPRESSION OF TFPI INHIBITS COAGULATION INITIATED BY ANTI-NONGAL IgG BINDING TO PORCINE AORTIC ENDOTHELIAL CELLS
    Article Snippet: To investigate further, HI sensitized baboon serum was pretreated with an anti-human IgG Fab Ab for 30min at 4°C before incubation with PAECs. .. The anti-human IgG Fab Ab blocked the effect of HI sensitized baboon serum to induce WT PAEC TF activity ( ). ..

    FACS:

    Article Title: Host immunoglobulin G selectively identifies pathobionts in pediatric inflammatory bowel diseases
    Article Snippet: Samples were blocked (5% w :v BSA/2% v :v goat serum) for 30 min, washed, and then aliquoted for staining. .. For FACS Aria cell sorting and ImageStream image cytometry, samples were aliquoted to 3 groups: an unstained control, an IgG isotype control (Abcam; diluted 1:100; 2 μg/mL), and an anti-human IgG stained sample (Abcam; diluted 1:150; 3 μg/mL). .. Aliquots were washed and incubated with AlexaFluor 488-conjugated anti-rabbit secondary antibody (Invitrogen; diluted 1:300; 7 μg/mL).

    Cytometry:

    Article Title: Host immunoglobulin G selectively identifies pathobionts in pediatric inflammatory bowel diseases
    Article Snippet: Samples were blocked (5% w :v BSA/2% v :v goat serum) for 30 min, washed, and then aliquoted for staining. .. For FACS Aria cell sorting and ImageStream image cytometry, samples were aliquoted to 3 groups: an unstained control, an IgG isotype control (Abcam; diluted 1:100; 2 μg/mL), and an anti-human IgG stained sample (Abcam; diluted 1:150; 3 μg/mL). .. Aliquots were washed and incubated with AlexaFluor 488-conjugated anti-rabbit secondary antibody (Invitrogen; diluted 1:300; 7 μg/mL).

    Staining:

    Article Title: Host immunoglobulin G selectively identifies pathobionts in pediatric inflammatory bowel diseases
    Article Snippet: Samples were blocked (5% w :v BSA/2% v :v goat serum) for 30 min, washed, and then aliquoted for staining. .. For FACS Aria cell sorting and ImageStream image cytometry, samples were aliquoted to 3 groups: an unstained control, an IgG isotype control (Abcam; diluted 1:100; 2 μg/mL), and an anti-human IgG stained sample (Abcam; diluted 1:150; 3 μg/mL). .. Aliquots were washed and incubated with AlexaFluor 488-conjugated anti-rabbit secondary antibody (Invitrogen; diluted 1:300; 7 μg/mL).

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  • 95
    Abcam rabbit anti mouse α sma antibody
    Delayed wound healing in bone marrow KLF4 knockout mice and compromised accumulation of CCR2+MDSCs and fibrocytes (a). Chimeric mice receiving bone marrow cells from Rosa26CreER/KLF4(flox)/β-actin-EGFP donor mice were used. Quantification of the wound size in each group of mice is shown (n=10). (b). Single cells from the skin wound were gated by EGFP. They were examined by CD11b and Ly6G antibodies, followed by further analysis using a CCR2 antibody. Representative contour plots in each group are shown. (c). Similar to (b) except COL1A1, CD45, and CD11b antibodies were used to analyze the fibrocytes. (d). Representative immunofluorescent staining of the wounds with <t>α-SMA</t> and COL1A1 antibodies. Yellow arrows indicate EGFP/α-SMA or EGFP/COL1A1 co-expressing cells (Scale bars: 50 μm).
    Rabbit Anti Mouse α Sma Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mouse α sma antibody/product/Abcam
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti mouse α sma antibody - by Bioz Stars, 2021-07
    95/100 stars
      Buy from Supplier

    99
    Abcam anti α sma mouse monoclonal antibody
    Histopathological analysis and immunohistochemical staining. Following DMN injection, rats were orally given distilled water, CGXII (50 mg and 100 mg/kg), or DDB (50 mg/kg) daily for 4 weeks. The liver tissues were examined using hematoxylin and eosin (a), Masson's trichrome (b), and immunohistochemistry for  α -SMA (c); pathophysiologic examinations were performed under light microscopy (100x magnification). The inflammation scores (d), METAVIR scores (e), and the  α -SMA positive cells (f) were analyzed. Data are expressed as the mean ± SD ( n  = 6).  ### p
    Anti α Sma Mouse Monoclonal Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti α sma mouse monoclonal antibody/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti α sma mouse monoclonal antibody - by Bioz Stars, 2021-07
    99/100 stars
      Buy from Supplier


    Image Search Results


    Delayed wound healing in bone marrow KLF4 knockout mice and compromised accumulation of CCR2+MDSCs and fibrocytes (a). Chimeric mice receiving bone marrow cells from Rosa26CreER/KLF4(flox)/β-actin-EGFP donor mice were used. Quantification of the wound size in each group of mice is shown (n=10). (b). Single cells from the skin wound were gated by EGFP. They were examined by CD11b and Ly6G antibodies, followed by further analysis using a CCR2 antibody. Representative contour plots in each group are shown. (c). Similar to (b) except COL1A1, CD45, and CD11b antibodies were used to analyze the fibrocytes. (d). Representative immunofluorescent staining of the wounds with α-SMA and COL1A1 antibodies. Yellow arrows indicate EGFP/α-SMA or EGFP/COL1A1 co-expressing cells (Scale bars: 50 μm).

    Journal: The Journal of investigative dermatology

    Article Title: Kruppel like factor KLF4 facilitates cutaneous wound healing by promoting fibrocyte generation from myeloid-derived suppressor cells

    doi: 10.1038/jid.2015.3

    Figure Lengend Snippet: Delayed wound healing in bone marrow KLF4 knockout mice and compromised accumulation of CCR2+MDSCs and fibrocytes (a). Chimeric mice receiving bone marrow cells from Rosa26CreER/KLF4(flox)/β-actin-EGFP donor mice were used. Quantification of the wound size in each group of mice is shown (n=10). (b). Single cells from the skin wound were gated by EGFP. They were examined by CD11b and Ly6G antibodies, followed by further analysis using a CCR2 antibody. Representative contour plots in each group are shown. (c). Similar to (b) except COL1A1, CD45, and CD11b antibodies were used to analyze the fibrocytes. (d). Representative immunofluorescent staining of the wounds with α-SMA and COL1A1 antibodies. Yellow arrows indicate EGFP/α-SMA or EGFP/COL1A1 co-expressing cells (Scale bars: 50 μm).

    Article Snippet: The following antibodies were used: rabbit anti-mouse KLF4 antibody (1:100), rabbit anti-mouse FSP-1 antibody (1:100) and rabbit anti-mouse α-SMA antibody (1:100, both from Abcam, ) and rabbit anti-mouse Collagen Type I alpha 1 antibody (COL1A1, 1:200, Rockland).

    Techniques: Knock-Out, Mouse Assay, Staining, Expressing

    KLF4-expressing bone marrow cells are integrated into the healing tissue and co-localized with α-SMA-expressing cells Chimeric mice were generated by bone marrow transplantation using bone marrow cells from C57BL/6 mice (Control) or KLF4/EGFP mice into C57BL/6 recipient mice. 8mm diameter full thickness wound was placed and the wound beds were collected 4 days later followed by immunofluorescence staining with an anti-α-SMA antibody. Representative co-localization of EGFP cells with red α-SMA-expressing cells in the healing tissue (left) are indicated by white arrowheads (n=5). Scale bars: 25 μm.

    Journal: The Journal of investigative dermatology

    Article Title: Kruppel like factor KLF4 facilitates cutaneous wound healing by promoting fibrocyte generation from myeloid-derived suppressor cells

    doi: 10.1038/jid.2015.3

    Figure Lengend Snippet: KLF4-expressing bone marrow cells are integrated into the healing tissue and co-localized with α-SMA-expressing cells Chimeric mice were generated by bone marrow transplantation using bone marrow cells from C57BL/6 mice (Control) or KLF4/EGFP mice into C57BL/6 recipient mice. 8mm diameter full thickness wound was placed and the wound beds were collected 4 days later followed by immunofluorescence staining with an anti-α-SMA antibody. Representative co-localization of EGFP cells with red α-SMA-expressing cells in the healing tissue (left) are indicated by white arrowheads (n=5). Scale bars: 25 μm.

    Article Snippet: The following antibodies were used: rabbit anti-mouse KLF4 antibody (1:100), rabbit anti-mouse FSP-1 antibody (1:100) and rabbit anti-mouse α-SMA antibody (1:100, both from Abcam, ) and rabbit anti-mouse Collagen Type I alpha 1 antibody (COL1A1, 1:200, Rockland).

    Techniques: Expressing, Mouse Assay, Generated, Transplantation Assay, Immunofluorescence, Staining

    KLF4 ablation delayed cutaneous wound healing accompanied by decreased accumulation of MDSCs (a). KLF4 IHC staining in skin of RosaCreER/KLF4(flox) mice without (KLF4+/+) and with tamoxifen induction (KLF4−/−). The areas between two dotted red lines delineate skin suprabasal layers. (b). qRT-PCR to analyze expression of KLF4 and CCR2 . (c). Wound healing phenotypes (left, n=10) and the wound size quantification (right). (d). IHC staining of α–SMA and COL1A1 in KLF4+/+ and KLF4−/− mice at Day 3 after wound induction. (e). Flow cytometry using anti-Ly6G and anti-CD11b antibodies with spleen cells and single skin cells. Representative images from one of five mice in each group are shown (a, c, and d). Scale bars: 50 μm. Mean ± SEM. *p

    Journal: The Journal of investigative dermatology

    Article Title: Kruppel like factor KLF4 facilitates cutaneous wound healing by promoting fibrocyte generation from myeloid-derived suppressor cells

    doi: 10.1038/jid.2015.3

    Figure Lengend Snippet: KLF4 ablation delayed cutaneous wound healing accompanied by decreased accumulation of MDSCs (a). KLF4 IHC staining in skin of RosaCreER/KLF4(flox) mice without (KLF4+/+) and with tamoxifen induction (KLF4−/−). The areas between two dotted red lines delineate skin suprabasal layers. (b). qRT-PCR to analyze expression of KLF4 and CCR2 . (c). Wound healing phenotypes (left, n=10) and the wound size quantification (right). (d). IHC staining of α–SMA and COL1A1 in KLF4+/+ and KLF4−/− mice at Day 3 after wound induction. (e). Flow cytometry using anti-Ly6G and anti-CD11b antibodies with spleen cells and single skin cells. Representative images from one of five mice in each group are shown (a, c, and d). Scale bars: 50 μm. Mean ± SEM. *p

    Article Snippet: The following antibodies were used: rabbit anti-mouse KLF4 antibody (1:100), rabbit anti-mouse FSP-1 antibody (1:100) and rabbit anti-mouse α-SMA antibody (1:100, both from Abcam, ) and rabbit anti-mouse Collagen Type I alpha 1 antibody (COL1A1, 1:200, Rockland).

    Techniques: Immunohistochemistry, Staining, Mouse Assay, Quantitative RT-PCR, Expressing, Flow Cytometry, Cytometry

    Histopathological analysis and immunohistochemical staining. Following DMN injection, rats were orally given distilled water, CGXII (50 mg and 100 mg/kg), or DDB (50 mg/kg) daily for 4 weeks. The liver tissues were examined using hematoxylin and eosin (a), Masson's trichrome (b), and immunohistochemistry for  α -SMA (c); pathophysiologic examinations were performed under light microscopy (100x magnification). The inflammation scores (d), METAVIR scores (e), and the  α -SMA positive cells (f) were analyzed. Data are expressed as the mean ± SD ( n  = 6).  ### p

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: A Herbal Formula, CGXII, Exerts Antihepatofibrotic Effect in Dimethylnitrosamine-Induced SD Rat Model

    doi: 10.1155/2016/5093718

    Figure Lengend Snippet: Histopathological analysis and immunohistochemical staining. Following DMN injection, rats were orally given distilled water, CGXII (50 mg and 100 mg/kg), or DDB (50 mg/kg) daily for 4 weeks. The liver tissues were examined using hematoxylin and eosin (a), Masson's trichrome (b), and immunohistochemistry for α -SMA (c); pathophysiologic examinations were performed under light microscopy (100x magnification). The inflammation scores (d), METAVIR scores (e), and the α -SMA positive cells (f) were analyzed. Data are expressed as the mean ± SD ( n = 6). ### p

    Article Snippet: The slides were then incubated overnight with an anti-α -SMA mouse monoclonal antibody (1 : 250, Abcam, Cambridge, UK) and incubated overnight.

    Techniques: Immunohistochemistry, Staining, Injection, Light Microscopy

    Trehalose reduced the expression of α-SMA and ColIα 1 mRNA expression by the stimulation with TGF-β 1 in PMCs. ( a ) The LDH release of cells following 24 h incubation with trehalose. LDH release were assessed by absorbance at 490 nm and expressed as relative proportions to control samples without trehalose ± SEM (n = 3 cell preparation/group). ( b , c ) TGF-β 1 induced α-SMA mRNA expression in a time- and dose-dependent manner (n = 3 cell preparation/group). ( d ) The effects of trehalose on TGF-β 1 -induced α-SMA mRNA expression (n = 3 cell preparation/group; T, trehalose; M, mannitol; L, l -glucose). PMCs were pre-treated with either trehalose, mannitol or l -glucose for 30 min prior to TGF-β 1 stimulation. ( e , f ) TGF-β 1 induced increase of ColIα1 mRNA expression in a time- and dose-dependent manner (n = 3 cell preparation/group). ( g ) The effects of trehalose on TGF-β 1 -induced ColIα 1 mRNA expression (n = 3 cell preparation/group). PMCs were pre-treated with either trehalose, mannitol or l -glucose for 30 min prior to TGF-β 1 stimulation. Data are expressed as mean ± SEM. Each experiment was performed two times independently.

    Journal: Scientific Reports

    Article Title: Trehalose ameliorates peritoneal fibrosis by promoting Snail degradation and inhibiting mesothelial-to-mesenchymal transition in mesothelial cells

    doi: 10.1038/s41598-020-71230-4

    Figure Lengend Snippet: Trehalose reduced the expression of α-SMA and ColIα 1 mRNA expression by the stimulation with TGF-β 1 in PMCs. ( a ) The LDH release of cells following 24 h incubation with trehalose. LDH release were assessed by absorbance at 490 nm and expressed as relative proportions to control samples without trehalose ± SEM (n = 3 cell preparation/group). ( b , c ) TGF-β 1 induced α-SMA mRNA expression in a time- and dose-dependent manner (n = 3 cell preparation/group). ( d ) The effects of trehalose on TGF-β 1 -induced α-SMA mRNA expression (n = 3 cell preparation/group; T, trehalose; M, mannitol; L, l -glucose). PMCs were pre-treated with either trehalose, mannitol or l -glucose for 30 min prior to TGF-β 1 stimulation. ( e , f ) TGF-β 1 induced increase of ColIα1 mRNA expression in a time- and dose-dependent manner (n = 3 cell preparation/group). ( g ) The effects of trehalose on TGF-β 1 -induced ColIα 1 mRNA expression (n = 3 cell preparation/group). PMCs were pre-treated with either trehalose, mannitol or l -glucose for 30 min prior to TGF-β 1 stimulation. Data are expressed as mean ± SEM. Each experiment was performed two times independently.

    Article Snippet: After incubation in PVDF blocking reagent (Toyobo, Osaka, Japan), membranes were incubated overnight at 4 °C with mouse anti-human α-SMA monoclonal antibody (Abcam), rabbit anti-mouse Smad2 monoclonal antibody (Cell Signaling), rabbit anti-mouse phospho-Smad2 monoclonal antibody (Cell Signaling), rabbit anti-mouse Smad3 monoclonal antibody (Cell Signaling), rabbit anti-mouse phospho-Smad3 monoclonal antibody (Cell Signaling), rabbit anti-mouse Snail monoclonal antibody (Cell Signaling), rabbit anti-mouse LC3A/B monoclonal antibody (Cell Signaling), rabbit anti-mouse JNK monoclonal antibody (Cell Signaling), rabbit anti-mouse phospho-JNK monoclonal antibody (Cell Signaling), rabbit anti-mouse p38-MAPK monoclonal antibody (Cell Signaling), rabbit anti-mouse phospho-p38MAPK monoclonal antibody (Cell Signaling), rabbit anti-mouse GSK3-β monoclonal antibody (Cell Signaling), rabbit anti-mouse phospho-GSK3-βmonoclonal antibody (Cell Signaling), rabbit anti-mouse β-actin monoclonal antibody (Cell Signaling) or anti-muse GAPDH monoclonal antibody (Cell signaling).

    Techniques: Expressing, Incubation

    Trehalose contributed to the reduction of peritoneal myofibroblast accumulation during fibrogenesis. ( a ) The localization and expression of α-SMA protein in the peritoneum. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( b ) Numbers of α-SMA positive cells in the peritoneum. Data are expressed as the mean number ± SEM per HPF (n = 3 mice/group). ( c ) Peritoneal expression of α-SMA mRNA at day 21 (n = 6 mice/group). ( d ) Peritoneal expression of α-SMA protein from mice following CG challenges (Day 21, n = 3 mice/group). ( e ) Peritoneal expression of TGF-β 1 mRNA at day 21 (n = 6 mice/group). Data are expressed as mean ± SEM.

    Journal: Scientific Reports

    Article Title: Trehalose ameliorates peritoneal fibrosis by promoting Snail degradation and inhibiting mesothelial-to-mesenchymal transition in mesothelial cells

    doi: 10.1038/s41598-020-71230-4

    Figure Lengend Snippet: Trehalose contributed to the reduction of peritoneal myofibroblast accumulation during fibrogenesis. ( a ) The localization and expression of α-SMA protein in the peritoneum. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( b ) Numbers of α-SMA positive cells in the peritoneum. Data are expressed as the mean number ± SEM per HPF (n = 3 mice/group). ( c ) Peritoneal expression of α-SMA mRNA at day 21 (n = 6 mice/group). ( d ) Peritoneal expression of α-SMA protein from mice following CG challenges (Day 21, n = 3 mice/group). ( e ) Peritoneal expression of TGF-β 1 mRNA at day 21 (n = 6 mice/group). Data are expressed as mean ± SEM.

    Article Snippet: After incubation in PVDF blocking reagent (Toyobo, Osaka, Japan), membranes were incubated overnight at 4 °C with mouse anti-human α-SMA monoclonal antibody (Abcam), rabbit anti-mouse Smad2 monoclonal antibody (Cell Signaling), rabbit anti-mouse phospho-Smad2 monoclonal antibody (Cell Signaling), rabbit anti-mouse Smad3 monoclonal antibody (Cell Signaling), rabbit anti-mouse phospho-Smad3 monoclonal antibody (Cell Signaling), rabbit anti-mouse Snail monoclonal antibody (Cell Signaling), rabbit anti-mouse LC3A/B monoclonal antibody (Cell Signaling), rabbit anti-mouse JNK monoclonal antibody (Cell Signaling), rabbit anti-mouse phospho-JNK monoclonal antibody (Cell Signaling), rabbit anti-mouse p38-MAPK monoclonal antibody (Cell Signaling), rabbit anti-mouse phospho-p38MAPK monoclonal antibody (Cell Signaling), rabbit anti-mouse GSK3-β monoclonal antibody (Cell Signaling), rabbit anti-mouse phospho-GSK3-βmonoclonal antibody (Cell Signaling), rabbit anti-mouse β-actin monoclonal antibody (Cell Signaling) or anti-muse GAPDH monoclonal antibody (Cell signaling).

    Techniques: Expressing, Mouse Assay

    Trehalose had no effect on the protein degradation pathway related to the proteasome. ( a ) PMCs were pre-treated with trehalose for 30 min prior to TGF-β 1 stimulation. Trehalose did not alter the ratio of phosphorylated GSK3-β to total GSK3-β protein expression induced by TGF-β 1 . Data are expressed as mean density of phosphorylated GSK3-β bands relative to total GSK3-β bands ± SEM expression (n = 3 cell preparation/group). ( b , c ) PMCs were pre-treated with bortezomib for 1 h followed by 30 min pre-treatment with trehalose. Then, TGF-β 1 was added to PMCs for 24 h. Bortezomib did not influence the suppressive effects of trehalose on α-SMA and ColIα 1 mRNA expression induced by TGF-β 1 in PMCs (n = 3 cell preparation/group). Data are expressed as copies of α-SMA and ColIα 1 mRNA relative to copies of β 2 microgloblin mRNA ± SEM. Each experiment was performed two times independently.

    Journal: Scientific Reports

    Article Title: Trehalose ameliorates peritoneal fibrosis by promoting Snail degradation and inhibiting mesothelial-to-mesenchymal transition in mesothelial cells

    doi: 10.1038/s41598-020-71230-4

    Figure Lengend Snippet: Trehalose had no effect on the protein degradation pathway related to the proteasome. ( a ) PMCs were pre-treated with trehalose for 30 min prior to TGF-β 1 stimulation. Trehalose did not alter the ratio of phosphorylated GSK3-β to total GSK3-β protein expression induced by TGF-β 1 . Data are expressed as mean density of phosphorylated GSK3-β bands relative to total GSK3-β bands ± SEM expression (n = 3 cell preparation/group). ( b , c ) PMCs were pre-treated with bortezomib for 1 h followed by 30 min pre-treatment with trehalose. Then, TGF-β 1 was added to PMCs for 24 h. Bortezomib did not influence the suppressive effects of trehalose on α-SMA and ColIα 1 mRNA expression induced by TGF-β 1 in PMCs (n = 3 cell preparation/group). Data are expressed as copies of α-SMA and ColIα 1 mRNA relative to copies of β 2 microgloblin mRNA ± SEM. Each experiment was performed two times independently.

    Article Snippet: After incubation in PVDF blocking reagent (Toyobo, Osaka, Japan), membranes were incubated overnight at 4 °C with mouse anti-human α-SMA monoclonal antibody (Abcam), rabbit anti-mouse Smad2 monoclonal antibody (Cell Signaling), rabbit anti-mouse phospho-Smad2 monoclonal antibody (Cell Signaling), rabbit anti-mouse Smad3 monoclonal antibody (Cell Signaling), rabbit anti-mouse phospho-Smad3 monoclonal antibody (Cell Signaling), rabbit anti-mouse Snail monoclonal antibody (Cell Signaling), rabbit anti-mouse LC3A/B monoclonal antibody (Cell Signaling), rabbit anti-mouse JNK monoclonal antibody (Cell Signaling), rabbit anti-mouse phospho-JNK monoclonal antibody (Cell Signaling), rabbit anti-mouse p38-MAPK monoclonal antibody (Cell Signaling), rabbit anti-mouse phospho-p38MAPK monoclonal antibody (Cell Signaling), rabbit anti-mouse GSK3-β monoclonal antibody (Cell Signaling), rabbit anti-mouse phospho-GSK3-βmonoclonal antibody (Cell Signaling), rabbit anti-mouse β-actin monoclonal antibody (Cell Signaling) or anti-muse GAPDH monoclonal antibody (Cell signaling).

    Techniques: Expressing

    Trehalose suppressed the accumulation of myofibroblasts derived from PMCs. ( a ) Accumulation of myofibroblasts derived from PMCs (α-SMA + Wt + , Wt1; Wilms tumor 1). Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( b , c ) Numbers of α-SMA + WT1 + cells ( b ) and percentage of α-SMA + WT1 + /total α-SMA + cells ( c ) in the peritoneum. ( d ) The localization of mesothelin protein in the peritoneum. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( e ) Numbers of mesothelin-positive cells in the peritoneum. Data are expressed as the mean number ± SEM per HPF (n = 3 mice/group).

    Journal: Scientific Reports

    Article Title: Trehalose ameliorates peritoneal fibrosis by promoting Snail degradation and inhibiting mesothelial-to-mesenchymal transition in mesothelial cells

    doi: 10.1038/s41598-020-71230-4

    Figure Lengend Snippet: Trehalose suppressed the accumulation of myofibroblasts derived from PMCs. ( a ) Accumulation of myofibroblasts derived from PMCs (α-SMA + Wt + , Wt1; Wilms tumor 1). Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( b , c ) Numbers of α-SMA + WT1 + cells ( b ) and percentage of α-SMA + WT1 + /total α-SMA + cells ( c ) in the peritoneum. ( d ) The localization of mesothelin protein in the peritoneum. Representative tissue sections of mice following CG challenges (Day 21) (magnification × 200). Bars, 100 μm. ( e ) Numbers of mesothelin-positive cells in the peritoneum. Data are expressed as the mean number ± SEM per HPF (n = 3 mice/group).

    Article Snippet: After incubation in PVDF blocking reagent (Toyobo, Osaka, Japan), membranes were incubated overnight at 4 °C with mouse anti-human α-SMA monoclonal antibody (Abcam), rabbit anti-mouse Smad2 monoclonal antibody (Cell Signaling), rabbit anti-mouse phospho-Smad2 monoclonal antibody (Cell Signaling), rabbit anti-mouse Smad3 monoclonal antibody (Cell Signaling), rabbit anti-mouse phospho-Smad3 monoclonal antibody (Cell Signaling), rabbit anti-mouse Snail monoclonal antibody (Cell Signaling), rabbit anti-mouse LC3A/B monoclonal antibody (Cell Signaling), rabbit anti-mouse JNK monoclonal antibody (Cell Signaling), rabbit anti-mouse phospho-JNK monoclonal antibody (Cell Signaling), rabbit anti-mouse p38-MAPK monoclonal antibody (Cell Signaling), rabbit anti-mouse phospho-p38MAPK monoclonal antibody (Cell Signaling), rabbit anti-mouse GSK3-β monoclonal antibody (Cell Signaling), rabbit anti-mouse phospho-GSK3-βmonoclonal antibody (Cell Signaling), rabbit anti-mouse β-actin monoclonal antibody (Cell Signaling) or anti-muse GAPDH monoclonal antibody (Cell signaling).

    Techniques: Derivative Assay, Wilms Tumor Assay, Mouse Assay

    Morphological features and α-SMA expression of cultured hepatic stellate cells during the activation process. Primary hepatic stellate cells (HSCs) were maintained on uncoated plastic dishes. HSCs presented a star-shaped appearance with fewer

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Early activated hepatic stellate cell-derived molecules reverse acute hepatic injury

    doi: 10.3748/wjg.v21.i14.4184

    Figure Lengend Snippet: Morphological features and α-SMA expression of cultured hepatic stellate cells during the activation process. Primary hepatic stellate cells (HSCs) were maintained on uncoated plastic dishes. HSCs presented a star-shaped appearance with fewer

    Article Snippet: After blocking with 5% BSA (Sigma Aldrich, St. Louis, MO, United States) in PBS, cells were incubated with mouse monoclonal anti-α-SMA IgG (Abcam, Cambridge, MA; 1:100) or rabbit anti-desmin (Abcam, Cambridge, MA; 1:100) for 2 h at room temperature, followed by development with donkey anti-mouse Alexa Fluor 488 (Invitrogen, Carlsbad, California, United States; 1:500) or donkey anti-rabbit Alexa Fluor 594 (Invitrogen, Carlsbad, California, United States; 1:500) for 30 min at room temperature, respectively.

    Techniques: Expressing, Cell Culture, Activation Assay