anti p ampk thr172  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti p ampk thr172
    Anti P Ampk Thr172, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti p ampk thr172 - by Bioz Stars, 2023-06
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    anti p ampk thr172  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Cell Signaling Technology Inc anti p ampk thr172
    Anti P Ampk Thr172, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p ampk thr172/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p ampk thr172 - by Bioz Stars, 2023-06
    96/100 stars

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    phosphor p ampk thr172  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phosphor p ampk thr172
    CUR regulates energy metabolism through AMPK-dependent pathways in PRV-infected BV2 cells. A BV2 cells were infected with/without PRV for 24 h and then treated with/without 20 μM CUR for 24 h. Western blot analysis of p-AMPK <t>Thr172</t> protein levels (normalized to the t-AMPK protein). β-Actin was used as the loading control ( n = 3). B Relative protein levels of p-AMPK Thr172 . C BV2 cells were treated with different concentrations of Compound C for 24 h, and cell viability was determined by the CCK-8 assay to determine the toxicity range ( n = 4). D BV2 cells were infected with/without PRV or were treated with PRV and an AMPK inhibitor (Compound C or siRNA) alone or in combination for 24 h, followed by treatment with/without 20 μM CUR for 24 h. Western blot analysis of p-AMPK (normalized to the t-AMPK protein), t-AMPK, Gpat4, and LDHa levels (Gpat4 and LDHa values were normalized to the β-actin protein); β-actin was used as the loading control ( n = 3). E Relative p-AMPKThr172 protein levels. F Relative LDHa protein levels. G Relative Gpat4 protein levels. H The extracellular acidification rate of BV2 cells after the different treatments (values normalized to the control) ( n = 4). I The OCR of BV2 cells after the different treatments (values normalized to the control) ( n = 4). J BV2 cell ATP levels in the different treatment groups ( n = 4). K The M1 phenotype-related inflammatory factor TNF-α in BV2 cells was detected using ELISA ( n = 4). L The levels of the M1 phenotype-related inflammatory factor IL-6 in BV2 cells ( n = 4). M The levels of the M2 phenotype-related anti-inflammatory factor IL-4 in BV2 cells ( n = 4). N The levels of the M2 phenotype-related anti-inflammatory factor IL-10 in BV2 cells ( n = 4). All experiments were performed in parallel. The results are presented as the mean ± SD. Statistical significance was determined using one-way ANOVA followed by an LSD post hoc test for multiple comparisons among the groups. * P < 0.05, ** P < 0.01, *** P < 0.001, and NS, not significant.
    Phosphor P Ampk Thr172, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphor p ampk thr172/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphor p ampk thr172 - by Bioz Stars, 2023-06
    96/100 stars

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    1) Product Images from "Curcumin-dependent phenotypic transformation of microglia mediates resistance to pseudorabies-induced encephalitis"

    Article Title: Curcumin-dependent phenotypic transformation of microglia mediates resistance to pseudorabies-induced encephalitis

    Journal: Veterinary Research

    doi: 10.1186/s13567-023-01149-x

    CUR regulates energy metabolism through AMPK-dependent pathways in PRV-infected BV2 cells. A BV2 cells were infected with/without PRV for 24 h and then treated with/without 20 μM CUR for 24 h. Western blot analysis of p-AMPK Thr172 protein levels (normalized to the t-AMPK protein). β-Actin was used as the loading control ( n = 3). B Relative protein levels of p-AMPK Thr172 . C BV2 cells were treated with different concentrations of Compound C for 24 h, and cell viability was determined by the CCK-8 assay to determine the toxicity range ( n = 4). D BV2 cells were infected with/without PRV or were treated with PRV and an AMPK inhibitor (Compound C or siRNA) alone or in combination for 24 h, followed by treatment with/without 20 μM CUR for 24 h. Western blot analysis of p-AMPK (normalized to the t-AMPK protein), t-AMPK, Gpat4, and LDHa levels (Gpat4 and LDHa values were normalized to the β-actin protein); β-actin was used as the loading control ( n = 3). E Relative p-AMPKThr172 protein levels. F Relative LDHa protein levels. G Relative Gpat4 protein levels. H The extracellular acidification rate of BV2 cells after the different treatments (values normalized to the control) ( n = 4). I The OCR of BV2 cells after the different treatments (values normalized to the control) ( n = 4). J BV2 cell ATP levels in the different treatment groups ( n = 4). K The M1 phenotype-related inflammatory factor TNF-α in BV2 cells was detected using ELISA ( n = 4). L The levels of the M1 phenotype-related inflammatory factor IL-6 in BV2 cells ( n = 4). M The levels of the M2 phenotype-related anti-inflammatory factor IL-4 in BV2 cells ( n = 4). N The levels of the M2 phenotype-related anti-inflammatory factor IL-10 in BV2 cells ( n = 4). All experiments were performed in parallel. The results are presented as the mean ± SD. Statistical significance was determined using one-way ANOVA followed by an LSD post hoc test for multiple comparisons among the groups. * P < 0.05, ** P < 0.01, *** P < 0.001, and NS, not significant.
    Figure Legend Snippet: CUR regulates energy metabolism through AMPK-dependent pathways in PRV-infected BV2 cells. A BV2 cells were infected with/without PRV for 24 h and then treated with/without 20 μM CUR for 24 h. Western blot analysis of p-AMPK Thr172 protein levels (normalized to the t-AMPK protein). β-Actin was used as the loading control ( n = 3). B Relative protein levels of p-AMPK Thr172 . C BV2 cells were treated with different concentrations of Compound C for 24 h, and cell viability was determined by the CCK-8 assay to determine the toxicity range ( n = 4). D BV2 cells were infected with/without PRV or were treated with PRV and an AMPK inhibitor (Compound C or siRNA) alone or in combination for 24 h, followed by treatment with/without 20 μM CUR for 24 h. Western blot analysis of p-AMPK (normalized to the t-AMPK protein), t-AMPK, Gpat4, and LDHa levels (Gpat4 and LDHa values were normalized to the β-actin protein); β-actin was used as the loading control ( n = 3). E Relative p-AMPKThr172 protein levels. F Relative LDHa protein levels. G Relative Gpat4 protein levels. H The extracellular acidification rate of BV2 cells after the different treatments (values normalized to the control) ( n = 4). I The OCR of BV2 cells after the different treatments (values normalized to the control) ( n = 4). J BV2 cell ATP levels in the different treatment groups ( n = 4). K The M1 phenotype-related inflammatory factor TNF-α in BV2 cells was detected using ELISA ( n = 4). L The levels of the M1 phenotype-related inflammatory factor IL-6 in BV2 cells ( n = 4). M The levels of the M2 phenotype-related anti-inflammatory factor IL-4 in BV2 cells ( n = 4). N The levels of the M2 phenotype-related anti-inflammatory factor IL-10 in BV2 cells ( n = 4). All experiments were performed in parallel. The results are presented as the mean ± SD. Statistical significance was determined using one-way ANOVA followed by an LSD post hoc test for multiple comparisons among the groups. * P < 0.05, ** P < 0.01, *** P < 0.001, and NS, not significant.

    Techniques Used: Infection, Western Blot, CCK-8 Assay, Enzyme-linked Immunosorbent Assay

    rabbit anti p ampk  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti p ampk
    Rabbit Anti P Ampk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti phosphot172 ampkα antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phosphot172 ampkα antibody
    Anti Phosphot172 Ampkα Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phosphot172 ampkα antibody/product/Cell Signaling Technology Inc
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti phosphot172 ampkα antibody - by Bioz Stars, 2023-06
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