anti human il 17  (Thermo Fisher)


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    Name:
    IL 17A Monoclonal Antibody 4H1524
    Description:
    IL 17A Monoclonal Antibody for Flow
    Catalog Number:
    A18695
    Price:
    None
    Category:
    Antibodies Secondary Detection Reagents
    Applications:
    Cell Analysis|Flow Antibodies for Cytokines, Chemokines, Growth Factors & Hormones|Flow Cytometry Antibodies & Secondary Detection|Flow Cytometry
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    Structured Review

    Thermo Fisher anti human il 17
    Identification of age-dependent fungi specific multifunctional Th1/Th17 cells. ( A ) CD4 + CD45RA + CD31 + T cells from neonates, infants and children of different age groups, and adults were co-cultured with monocytes pulsed with C . albicans (left panel) or A . fumigatus (right panel). The frequency of T cells expressing intracellular <t>IL-17</t> (white), IFNγ (black) and both IL-17/IFNγ (grey) were determined by flow cytometry at the indicated time points, analysed by boolean gating and shown as different fractions of cytokine expressing cells in a stacked bar chart. ( B ) Frequency of naïve (CD4 + CD45RA + CD31 + ) or memory (CD4 + CD45RO + ) T cells of adults expressing intracellular IL-17 (white), IFNγ (black) and both IL-17/IFNγ (grey) were determined by flow cytometry at the indicated time points and analysed as described in ( A ). ( C ) Frequency of CD4 + CD45RA + CD31 + T cells from neonates and adults expressing Th1 and Th17 transcription factors T-bet, RORγt and both T-bet as well as RORγt following stimulation with C . albicans (orange bars) or A . fumigatus (blue bars) for 3 and 6 days as described in ( A ). Cumulative results are shown and each dot represents a different donor. The error bars in figures denote ± SD. *p
    IL 17A Monoclonal Antibody for Flow
    https://www.bioz.com/result/anti human il 17/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti human il 17 - by Bioz Stars, 2021-06
    93/100 stars

    Images

    1) Product Images from "Developmental induction of human T-cell responses against Candida albicans and Aspergillus fumigatus"

    Article Title: Developmental induction of human T-cell responses against Candida albicans and Aspergillus fumigatus

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-35161-5

    Identification of age-dependent fungi specific multifunctional Th1/Th17 cells. ( A ) CD4 + CD45RA + CD31 + T cells from neonates, infants and children of different age groups, and adults were co-cultured with monocytes pulsed with C . albicans (left panel) or A . fumigatus (right panel). The frequency of T cells expressing intracellular IL-17 (white), IFNγ (black) and both IL-17/IFNγ (grey) were determined by flow cytometry at the indicated time points, analysed by boolean gating and shown as different fractions of cytokine expressing cells in a stacked bar chart. ( B ) Frequency of naïve (CD4 + CD45RA + CD31 + ) or memory (CD4 + CD45RO + ) T cells of adults expressing intracellular IL-17 (white), IFNγ (black) and both IL-17/IFNγ (grey) were determined by flow cytometry at the indicated time points and analysed as described in ( A ). ( C ) Frequency of CD4 + CD45RA + CD31 + T cells from neonates and adults expressing Th1 and Th17 transcription factors T-bet, RORγt and both T-bet as well as RORγt following stimulation with C . albicans (orange bars) or A . fumigatus (blue bars) for 3 and 6 days as described in ( A ). Cumulative results are shown and each dot represents a different donor. The error bars in figures denote ± SD. *p
    Figure Legend Snippet: Identification of age-dependent fungi specific multifunctional Th1/Th17 cells. ( A ) CD4 + CD45RA + CD31 + T cells from neonates, infants and children of different age groups, and adults were co-cultured with monocytes pulsed with C . albicans (left panel) or A . fumigatus (right panel). The frequency of T cells expressing intracellular IL-17 (white), IFNγ (black) and both IL-17/IFNγ (grey) were determined by flow cytometry at the indicated time points, analysed by boolean gating and shown as different fractions of cytokine expressing cells in a stacked bar chart. ( B ) Frequency of naïve (CD4 + CD45RA + CD31 + ) or memory (CD4 + CD45RO + ) T cells of adults expressing intracellular IL-17 (white), IFNγ (black) and both IL-17/IFNγ (grey) were determined by flow cytometry at the indicated time points and analysed as described in ( A ). ( C ) Frequency of CD4 + CD45RA + CD31 + T cells from neonates and adults expressing Th1 and Th17 transcription factors T-bet, RORγt and both T-bet as well as RORγt following stimulation with C . albicans (orange bars) or A . fumigatus (blue bars) for 3 and 6 days as described in ( A ). Cumulative results are shown and each dot represents a different donor. The error bars in figures denote ± SD. *p

    Techniques Used: Cell Culture, Expressing, Flow Cytometry, Cytometry

    Fungi specific T cells produce IL-17 in an age dependent manner. CD4 + CD45RA + CD31 + T cells from neonates, infants, children, and adults were co-cultured with monocytes pulsed with C . albicans- or A . fumigatus- lysates. ( A , B ) The frequency of T cells expressing signature Th17 molecules IL-17 ( A ) and RORγt ( B ) were analysed by flow cytometry at day 3 (upper Panel) and day 6 after stimulation (lower panel) ( C ) Bar graph representing the ELISPOT analysis of the quantitative IL-17, produced by the T cells from neonates and adults which were either stimulated or not for 3 days as described in ( A ). ( D ) Determination of IL-17A cytokine release of CD4 + CD45RA + T cells of neonates, infants and children or adults by LegendPlex which were either stimulated or not for 3 days as described in ( A ). ( E ) Frequency of T cells expressing intracellular IL-17 (white), IL-4 (black) and both IL-17/IL-4 (grey) were measured by flow cytometry after 6 days of stimulation, analysed by boolean gating and shown as different fractions of cytokine expressing cells in a stacked bar chart. (F) Bar graph showing IL-17 expression by CD4 + CD45RA + CD31 + T cells of neonates, adults, and infants of 0.5-2 years old, stimulated for 6 days as described in ( A ) in the presence or absence of neutralizing antibodies for IL-1ß, IL-6 or both. Cumulative results are shown and each dot in ( A – D ) and ( F ) represent a different donor. The error bars in figures denote ± SD. *p
    Figure Legend Snippet: Fungi specific T cells produce IL-17 in an age dependent manner. CD4 + CD45RA + CD31 + T cells from neonates, infants, children, and adults were co-cultured with monocytes pulsed with C . albicans- or A . fumigatus- lysates. ( A , B ) The frequency of T cells expressing signature Th17 molecules IL-17 ( A ) and RORγt ( B ) were analysed by flow cytometry at day 3 (upper Panel) and day 6 after stimulation (lower panel) ( C ) Bar graph representing the ELISPOT analysis of the quantitative IL-17, produced by the T cells from neonates and adults which were either stimulated or not for 3 days as described in ( A ). ( D ) Determination of IL-17A cytokine release of CD4 + CD45RA + T cells of neonates, infants and children or adults by LegendPlex which were either stimulated or not for 3 days as described in ( A ). ( E ) Frequency of T cells expressing intracellular IL-17 (white), IL-4 (black) and both IL-17/IL-4 (grey) were measured by flow cytometry after 6 days of stimulation, analysed by boolean gating and shown as different fractions of cytokine expressing cells in a stacked bar chart. (F) Bar graph showing IL-17 expression by CD4 + CD45RA + CD31 + T cells of neonates, adults, and infants of 0.5-2 years old, stimulated for 6 days as described in ( A ) in the presence or absence of neutralizing antibodies for IL-1ß, IL-6 or both. Cumulative results are shown and each dot in ( A – D ) and ( F ) represent a different donor. The error bars in figures denote ± SD. *p

    Techniques Used: Cell Culture, Expressing, Flow Cytometry, Cytometry, Enzyme-linked Immunospot, Produced

    2) Product Images from "Molecular Determinants of T Cell Epitope Recognition to the Common Timothy Grass Allergen"

    Article Title: Molecular Determinants of T Cell Epitope Recognition to the Common Timothy Grass Allergen

    Journal: Journal of Immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1000405

    Cytokine and Ig response patterns in donor cohorts. The patterns of IL-5, IFN-γ, IL-10, and IL-17 responses in the allergic, nonallergic, and SIT donor cohorts are shown in A and B . The majority of the responses, normalized for the size of the
    Figure Legend Snippet: Cytokine and Ig response patterns in donor cohorts. The patterns of IL-5, IFN-γ, IL-10, and IL-17 responses in the allergic, nonallergic, and SIT donor cohorts are shown in A and B . The majority of the responses, normalized for the size of the

    Techniques Used:

    Comparison of cytokine and IgE responses in allergic donors. The relationship between total CD4 + T cell cytokine response (IL-5, IFN-γ, IL-17, and IL-10) to overlapping peptides derived from Phl p 1, 2, 3, 4, and 5 are compared with the IgE serum
    Figure Legend Snippet: Comparison of cytokine and IgE responses in allergic donors. The relationship between total CD4 + T cell cytokine response (IL-5, IFN-γ, IL-17, and IL-10) to overlapping peptides derived from Phl p 1, 2, 3, 4, and 5 are compared with the IgE serum

    Techniques Used: Derivative Assay

    3) Product Images from "Stimulation with Concanavalin-A Induces IL-17 Production by Canine Peripheral T Cells"

    Article Title: Stimulation with Concanavalin-A Induces IL-17 Production by Canine Peripheral T Cells

    Journal: Veterinary Sciences

    doi: 10.3390/vetsci2020043

    Stimulation of canine peripheral T cells promotes IL-17 production. ( A ) Peripheral T cells were gated from unstimulated (left) or ConA-stimulated (right) PBMCs using light scatter properties to define lymphocyte regions. The top panel shows ungated PBMCs and the middle panel shows the lymphocyte population after the white blood cell population gates were defined. The bottom panel shows separation of CD4 + and CD8 + cells based on immunostaining. ( B ) IL-17 expression was determined by intracellular staining and flow cytometry in the CD4 + and CD8 + subsets from a . Two-dimensional dot plots show staining with isotype control antibodies, as well as staining of CD4 and CD8 cells with anti-IL-17 antibody in unstimulated (top) and ConA-stimulated (bottom) cells. These results represent Dog 1 shown in Table 1 . All dogs tested showed expression of IL-17 in at least one T cell subset (see Table 1 ).
    Figure Legend Snippet: Stimulation of canine peripheral T cells promotes IL-17 production. ( A ) Peripheral T cells were gated from unstimulated (left) or ConA-stimulated (right) PBMCs using light scatter properties to define lymphocyte regions. The top panel shows ungated PBMCs and the middle panel shows the lymphocyte population after the white blood cell population gates were defined. The bottom panel shows separation of CD4 + and CD8 + cells based on immunostaining. ( B ) IL-17 expression was determined by intracellular staining and flow cytometry in the CD4 + and CD8 + subsets from a . Two-dimensional dot plots show staining with isotype control antibodies, as well as staining of CD4 and CD8 cells with anti-IL-17 antibody in unstimulated (top) and ConA-stimulated (bottom) cells. These results represent Dog 1 shown in Table 1 . All dogs tested showed expression of IL-17 in at least one T cell subset (see Table 1 ).

    Techniques Used: Immunostaining, Expressing, Staining, Flow Cytometry, Cytometry

    Addition of TGF-β, IL-1β, and IL-6 increases polarization of CD4 + cells toward the Th17 phenotype. PBMCs from two dogs were stimulated as described in the main text and analyzed for polarization of T helper (CD4 + ) cells toward FoxP3 + (Treg) and IL-17 + (Th17) phenotypes using flow cytometry. CD4 + cells were identified as described in Figure 1 , and stimulation conditions are shown above the top panels, with isotype control staining shown for comparison in the ConA-stimulated samples. Gating was done using isotype control staining for stimulated cells, and MFI was calculated for all CD4 + T cells stained with IL-17 or with the matched isotype control (FL2). The MFI value for unstained cells in both dogs was 5.5.
    Figure Legend Snippet: Addition of TGF-β, IL-1β, and IL-6 increases polarization of CD4 + cells toward the Th17 phenotype. PBMCs from two dogs were stimulated as described in the main text and analyzed for polarization of T helper (CD4 + ) cells toward FoxP3 + (Treg) and IL-17 + (Th17) phenotypes using flow cytometry. CD4 + cells were identified as described in Figure 1 , and stimulation conditions are shown above the top panels, with isotype control staining shown for comparison in the ConA-stimulated samples. Gating was done using isotype control staining for stimulated cells, and MFI was calculated for all CD4 + T cells stained with IL-17 or with the matched isotype control (FL2). The MFI value for unstained cells in both dogs was 5.5.

    Techniques Used: Flow Cytometry, Cytometry, Staining

    Related Articles

    Staining:

    Article Title: IL-17 Genetic and Immunophenotypic Evaluation in Chronic Graft-versus-Host Disease
    Article Snippet: The cells were then fixed using 2% formaldehyde (Sigma-Aldrich). .. The fixed cells were permeabilized and stained, using phycoerythrin- (PE-) labeled anti-cytokine monoclonal antibodies for IL-17A (eBioscience, San Diego, CA, USA), IFN-γ (eBioscience, San Diego, CA, USA), or Foxp3 (eBioscience, San Diego, CA, USA), FITC- or PE-labeled isotype control antibodies and an unstimulated cell control were included in all experiments. .. Preparations were acquired on a FACSCanto II (Becton & Dickinson, San Jose, CA, USA).

    Article Title: The PD-1/PD-L1 inhibitory pathway is altered in pre-eclampsia and regulates T cell responses in pre-eclamptic rats
    Article Snippet: For intracellular cytokine detection, 2 × 106 cells were stimulated with 2 μL of Cell Stimulation Cocktail and 2 μL of Protein Transport Inhibitor Cocktail (eBioscience, San Diego, CA, USA) for 5 h. Collected cells were first stained with fluorescein-labelled mAbs for surface antigens, followed by fixation using IC Fixation buffer (eBioscience, San Diego, CA, USA). .. Then, the cells were stained with anti-human IL-17A-PE (eBioscience, San Diego, CA, USA) in an appropriate volume of 1 × Permeabilization Buffer (eBioscience, San Diego, CA, USA) for 25 min. .. Finally, the cells were re-suspended in 300 μL of PBS for subsequent flow cytometric analysis.

    Article Title: Interleukin-25 Mediates Transcriptional Control of PD-L1 via STAT3 in Multipotent Human Mesenchymal Stromal Cells (hMSCs) to Suppress Th17 Responses
    Article Snippet: Human recombinant IL-25 (rhIL-25; PeproTech) and various inhibitors (WP1066/InSolution STAT3 inhibitor III and Akt inhibitor III from Millipore; SP600125 JNK inhibitor and LY294002 PI3 kinase inhibitor from Cell Signaling Technology; and PD98059/MEK1/2 inhibitor from Cell Signaling Technology) were added to various experiments at the indicated doses after establishing toxicity profiles for monocytes and hMSCs. .. Flow Cytometry Cells were stained with antibodies as indicated: anti-human IL-25-PE (R & D Systems, IC1258P), mouse IgG1 isotype control-PE (R & D Systems, IC002P), anti-human CD3-PE/Cy5 (BioLegend, 300310), anti-human CD4-PE (BioLegend, 357404), anti-human IL-17A-PE (eBioscience, 12-7179), anti-human IFN-γ-FITC (BioLegend, 502506), anti-human IL-22-PE (eBioscience, 12-7229), anti-human FOXP3-Alexa Fluor 488 (BD Pharmingen, 561181), anti-human CD274 (B1-H1)-PE (eBioscience, 12-5983), mouse IgG1 isotype control-PE (eBioscience, 12-4714), anti-human IL-25R-PE (R & D Systems, FAB1207P), mouse IgG2b isotype control-PE (R & D Systems, IC0041P), anti-mouse CD4-APC (eBioscience, 17-0041), anti-mouse CD3e-PE/Cy5 (eBioscience, 15-0031), and anti-mouse/rat IL-17A-PE (eBioscience, 12-7177). ..

    Labeling:

    Article Title: IL-17 Genetic and Immunophenotypic Evaluation in Chronic Graft-versus-Host Disease
    Article Snippet: The cells were then fixed using 2% formaldehyde (Sigma-Aldrich). .. The fixed cells were permeabilized and stained, using phycoerythrin- (PE-) labeled anti-cytokine monoclonal antibodies for IL-17A (eBioscience, San Diego, CA, USA), IFN-γ (eBioscience, San Diego, CA, USA), or Foxp3 (eBioscience, San Diego, CA, USA), FITC- or PE-labeled isotype control antibodies and an unstimulated cell control were included in all experiments. .. Preparations were acquired on a FACSCanto II (Becton & Dickinson, San Jose, CA, USA).

    Enzyme-linked Immunospot:

    Article Title: Developmental induction of human T-cell responses against Candida albicans and Aspergillus fumigatus
    Article Snippet: All flow cytometric analyses were performed using FACS Canto II (BD Biosciences) together with FACSDiva software (BD Biosciences) to collect and compensate the data and FlowJo software (FlowJo LLC) for final data analysis. .. EliSpot assays ELISPOT plates (Millipore 96-well MultiScreen HA; Millipore) were coated with 2 mg/ml anti-human IL-17–specific mAb (eBio64CAP17; eBioscience) in PBS. .. Cells were cultured in the presence of PMA and ionomycin for 18 h at 37 °C/5% CO2 and plated at a density 1,25 × 105 cells/well in the ELISPOT plate.

    Incubation:

    Article Title: Licensing Virus-Specific T Cells to Secrete the Neutrophil Attracting Chemokine CXCL-8 during Hepatitis B Virus Infection
    Article Snippet: HBV peptides from the patients' respective genotype were added to a final concentration of 2 µg/ml and plates were incubated for 18 hours at 37°C. .. Following the incubation, IL-17 spot forming units (SFU) were detected using 0.25 µg/ml anti-human IL-17 MAb (eBio64DEC17; eBioscience) followed by incubation with streptavidin-alkaline phosphatase (Mabtech, Sweden). ..

    Expressing:

    Article Title: TLR2 Mediates Helicobacter pylori-Induced Tolerogenic Immune Response in Mice
    Article Snippet: Measurement of cytokine production by BMDCs and splenocytes The supernatant from the coculture of BMDCs and splenocytes was collected and the concentrations of IL-12p70, IL-23p19, IL-6, TNF-α, IFN-γ, IL-10, and IL-17A were measured using Quantikine ELISA Kits (eBioscience, San Diego, CA, or BD Biosciences, San Jose, CA). .. Determination of the expression of intracellular markers on splenocytes and surface markers on BMDCs by flow cytometry Splenocytes were labelled with fluorescence-conjugated antibodies to Foxp3, IFN-γ, and IL-17A (eBioscience). .. Stimulated BMDCs were dual-labelled with fluorescence-conjugated antibodies to CD11c and TLR2 or TLR4.

    Flow Cytometry:

    Article Title: TLR2 Mediates Helicobacter pylori-Induced Tolerogenic Immune Response in Mice
    Article Snippet: Measurement of cytokine production by BMDCs and splenocytes The supernatant from the coculture of BMDCs and splenocytes was collected and the concentrations of IL-12p70, IL-23p19, IL-6, TNF-α, IFN-γ, IL-10, and IL-17A were measured using Quantikine ELISA Kits (eBioscience, San Diego, CA, or BD Biosciences, San Jose, CA). .. Determination of the expression of intracellular markers on splenocytes and surface markers on BMDCs by flow cytometry Splenocytes were labelled with fluorescence-conjugated antibodies to Foxp3, IFN-γ, and IL-17A (eBioscience). .. Stimulated BMDCs were dual-labelled with fluorescence-conjugated antibodies to CD11c and TLR2 or TLR4.

    Article Title: Interleukin-25 Mediates Transcriptional Control of PD-L1 via STAT3 in Multipotent Human Mesenchymal Stromal Cells (hMSCs) to Suppress Th17 Responses
    Article Snippet: Human recombinant IL-25 (rhIL-25; PeproTech) and various inhibitors (WP1066/InSolution STAT3 inhibitor III and Akt inhibitor III from Millipore; SP600125 JNK inhibitor and LY294002 PI3 kinase inhibitor from Cell Signaling Technology; and PD98059/MEK1/2 inhibitor from Cell Signaling Technology) were added to various experiments at the indicated doses after establishing toxicity profiles for monocytes and hMSCs. .. Flow Cytometry Cells were stained with antibodies as indicated: anti-human IL-25-PE (R & D Systems, IC1258P), mouse IgG1 isotype control-PE (R & D Systems, IC002P), anti-human CD3-PE/Cy5 (BioLegend, 300310), anti-human CD4-PE (BioLegend, 357404), anti-human IL-17A-PE (eBioscience, 12-7179), anti-human IFN-γ-FITC (BioLegend, 502506), anti-human IL-22-PE (eBioscience, 12-7229), anti-human FOXP3-Alexa Fluor 488 (BD Pharmingen, 561181), anti-human CD274 (B1-H1)-PE (eBioscience, 12-5983), mouse IgG1 isotype control-PE (eBioscience, 12-4714), anti-human IL-25R-PE (R & D Systems, FAB1207P), mouse IgG2b isotype control-PE (R & D Systems, IC0041P), anti-mouse CD4-APC (eBioscience, 17-0041), anti-mouse CD3e-PE/Cy5 (eBioscience, 15-0031), and anti-mouse/rat IL-17A-PE (eBioscience, 12-7177). ..

    Cytometry:

    Article Title: TLR2 Mediates Helicobacter pylori-Induced Tolerogenic Immune Response in Mice
    Article Snippet: Measurement of cytokine production by BMDCs and splenocytes The supernatant from the coculture of BMDCs and splenocytes was collected and the concentrations of IL-12p70, IL-23p19, IL-6, TNF-α, IFN-γ, IL-10, and IL-17A were measured using Quantikine ELISA Kits (eBioscience, San Diego, CA, or BD Biosciences, San Jose, CA). .. Determination of the expression of intracellular markers on splenocytes and surface markers on BMDCs by flow cytometry Splenocytes were labelled with fluorescence-conjugated antibodies to Foxp3, IFN-γ, and IL-17A (eBioscience). .. Stimulated BMDCs were dual-labelled with fluorescence-conjugated antibodies to CD11c and TLR2 or TLR4.

    Article Title: Interleukin-25 Mediates Transcriptional Control of PD-L1 via STAT3 in Multipotent Human Mesenchymal Stromal Cells (hMSCs) to Suppress Th17 Responses
    Article Snippet: Human recombinant IL-25 (rhIL-25; PeproTech) and various inhibitors (WP1066/InSolution STAT3 inhibitor III and Akt inhibitor III from Millipore; SP600125 JNK inhibitor and LY294002 PI3 kinase inhibitor from Cell Signaling Technology; and PD98059/MEK1/2 inhibitor from Cell Signaling Technology) were added to various experiments at the indicated doses after establishing toxicity profiles for monocytes and hMSCs. .. Flow Cytometry Cells were stained with antibodies as indicated: anti-human IL-25-PE (R & D Systems, IC1258P), mouse IgG1 isotype control-PE (R & D Systems, IC002P), anti-human CD3-PE/Cy5 (BioLegend, 300310), anti-human CD4-PE (BioLegend, 357404), anti-human IL-17A-PE (eBioscience, 12-7179), anti-human IFN-γ-FITC (BioLegend, 502506), anti-human IL-22-PE (eBioscience, 12-7229), anti-human FOXP3-Alexa Fluor 488 (BD Pharmingen, 561181), anti-human CD274 (B1-H1)-PE (eBioscience, 12-5983), mouse IgG1 isotype control-PE (eBioscience, 12-4714), anti-human IL-25R-PE (R & D Systems, FAB1207P), mouse IgG2b isotype control-PE (R & D Systems, IC0041P), anti-mouse CD4-APC (eBioscience, 17-0041), anti-mouse CD3e-PE/Cy5 (eBioscience, 15-0031), and anti-mouse/rat IL-17A-PE (eBioscience, 12-7177). ..

    Fluorescence:

    Article Title: TLR2 Mediates Helicobacter pylori-Induced Tolerogenic Immune Response in Mice
    Article Snippet: Measurement of cytokine production by BMDCs and splenocytes The supernatant from the coculture of BMDCs and splenocytes was collected and the concentrations of IL-12p70, IL-23p19, IL-6, TNF-α, IFN-γ, IL-10, and IL-17A were measured using Quantikine ELISA Kits (eBioscience, San Diego, CA, or BD Biosciences, San Jose, CA). .. Determination of the expression of intracellular markers on splenocytes and surface markers on BMDCs by flow cytometry Splenocytes were labelled with fluorescence-conjugated antibodies to Foxp3, IFN-γ, and IL-17A (eBioscience). .. Stimulated BMDCs were dual-labelled with fluorescence-conjugated antibodies to CD11c and TLR2 or TLR4.

    Sandwich ELISA:

    Article Title: Reciprocal regulation of Th2 and Th17 cells by PAD2-mediated citrullination
    Article Snippet: .. Sandwich ELISA was performed with the following antibodies: anti–mouse IFN-γ (catalog 551221)/biotin anti–mouse IFN-γ (catalog 554550) from BD Pharmingen; anti–mouse IL-4 (catalog 554434)/biotin anti–mouse IL-4 (catalog 554390), anti–mouse IL-5 (catalog 554393)/biotin anti–mouse IL-5 (catalog 554397), anti–mouse IL-13 (catalog 14-7133-81)/biotin anti–mouse IL-13 (catalog 13-7135-81), anti–mouse IL-17A (catalog DY421)/biotin anti–mouse I L-17A (catalog DY421), anti–human IL-4 (catalog 14-7049)/biotin anti–human IL-4 (catalog 13-7048), and anti–human IL-17A (catalog 14-7178)/biotin anti–human IL-17A (catalog 13-7179) from eBioscience. .. Ovalbumin-specific IgE and IgG1 was quantified with anti–ovalbumin Ig E and anti–ovalbumin IgG1 ELISA kits (Cayman Chemical), respectively.

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  • 93
    Thermo Fisher anti human il 17
    Identification of age-dependent fungi specific multifunctional Th1/Th17 cells. ( A ) CD4 + CD45RA + CD31 + T cells from neonates, infants and children of different age groups, and adults were co-cultured with monocytes pulsed with C . albicans (left panel) or A . fumigatus (right panel). The frequency of T cells expressing intracellular <t>IL-17</t> (white), IFNγ (black) and both IL-17/IFNγ (grey) were determined by flow cytometry at the indicated time points, analysed by boolean gating and shown as different fractions of cytokine expressing cells in a stacked bar chart. ( B ) Frequency of naïve (CD4 + CD45RA + CD31 + ) or memory (CD4 + CD45RO + ) T cells of adults expressing intracellular IL-17 (white), IFNγ (black) and both IL-17/IFNγ (grey) were determined by flow cytometry at the indicated time points and analysed as described in ( A ). ( C ) Frequency of CD4 + CD45RA + CD31 + T cells from neonates and adults expressing Th1 and Th17 transcription factors T-bet, RORγt and both T-bet as well as RORγt following stimulation with C . albicans (orange bars) or A . fumigatus (blue bars) for 3 and 6 days as described in ( A ). Cumulative results are shown and each dot represents a different donor. The error bars in figures denote ± SD. *p
    Anti Human Il 17, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human il 17/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti human il 17 - by Bioz Stars, 2021-06
    93/100 stars
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    92
    Thermo Fisher phycoerythrin pe labeled anti human il 17a
    Regulation of interleukin (IL)-17 production in vitro . <t>IL-17A</t> levels (pg/ml) measured by enzyme-linked immunosorbent assay (ELISA) from supernatants taken from three different culture conditions in healthy individuals. Calculated P values are from two-tailed t -tests between IL-17 levels measured by ELISA from cultures containing anti-CD3, anti-CD3 plus Th17 stimulatory conditions and anti-CD3 plus Th17 stimulatory conditions with the addition of IL-4.
    Phycoerythrin Pe Labeled Anti Human Il 17a, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phycoerythrin pe labeled anti human il 17a/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phycoerythrin pe labeled anti human il 17a - by Bioz Stars, 2021-06
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    92
    Thermo Fisher il 17a monoclonal antibody
    Effects of rHMGB1 on apoptosis and differentiation of Treg and Th17 cells from normal coronary arteries (NCA) group. (A) and (B) Representative flow cytometric dot plots for apoptotic Treg cells (gated by CD4 + CD25 + CD127 - Annexin V + cells) and apoptotic Th17 cells (gated by CD3 + CD8 - IL-17 + Annexin V + cells) with or without 100 ng/mL rHMGB1 stimulation for 24 h in NCA group. (C) A summary of the percentage of apoptotic Treg and Th17 cells with or without 100 ng/mL rHMGB1 stimulation for 24 h in NCA group (n = 8); (D) Representative flow cytometric dot plots for purified naive CD4 + T cells (gated by CD4 + CD45RA + cells) from NCA group; (E) and (F) Representative flow cytometric dot plots for Treg cells (gated by CD4 + CD25 + CD127 - cells) and Th17 cells (gated by CD4 + IL-17 + cells) in blank (without induction), control (induction without rHMGB1 stimulation) and rHMGB1 (induction with 100 ng/mL rHMGB1 sitmulation) group; (G) Sum up Treg and Th17 cells frequencies after induction from naive CD4 + T cells with or without rHMGB1 stimulation (n = 6); (H) A summary of IL-10 and <t>IL-17A</t> levels in supernatants from each culture group (n = 6). (* p
    Il 17a Monoclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 17a monoclonal antibody/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    93
    Thermo Fisher prdm16
    Maternal exercise induces DNA demethylation of the <t>Prdm16</t> promoter through apelin/α-KG axis. ( A ) mRNA expression of Prdm16 of fetal BAT from M-Ctrl and M-Ex ( n = 6). Expression was normalized by Δ C t values. ( B ) Diagram showing three regions in the Prdm16 promoter. ( C , D , and H ) 5mC and 5hmC enrichment fold of fetal BAT (ME study, C; APN study, H) and adult offspring challenged with HFD (D) ( n = 6). ( E ) α-KG to 2-hydroxyglutarate (2-HG) ratio of fetal iBAT from ME or APN study ( n = 6). ( F , G , I , and J ) Z scores of mRNA level of ten-eleven translocation (Tet) and IDH families in the fetal iBAT of APN study (I and J) or ME study (F and G) ( n = 6). Expression was normalized by Δ C t values. ( K ) Working model: DNA demethylation stimulated by α-KG/AMPK/maternal exercise–induced apelin. Data are mean ± SEM, and each dot represents one litter. * P
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    Image Search Results


    Identification of age-dependent fungi specific multifunctional Th1/Th17 cells. ( A ) CD4 + CD45RA + CD31 + T cells from neonates, infants and children of different age groups, and adults were co-cultured with monocytes pulsed with C . albicans (left panel) or A . fumigatus (right panel). The frequency of T cells expressing intracellular IL-17 (white), IFNγ (black) and both IL-17/IFNγ (grey) were determined by flow cytometry at the indicated time points, analysed by boolean gating and shown as different fractions of cytokine expressing cells in a stacked bar chart. ( B ) Frequency of naïve (CD4 + CD45RA + CD31 + ) or memory (CD4 + CD45RO + ) T cells of adults expressing intracellular IL-17 (white), IFNγ (black) and both IL-17/IFNγ (grey) were determined by flow cytometry at the indicated time points and analysed as described in ( A ). ( C ) Frequency of CD4 + CD45RA + CD31 + T cells from neonates and adults expressing Th1 and Th17 transcription factors T-bet, RORγt and both T-bet as well as RORγt following stimulation with C . albicans (orange bars) or A . fumigatus (blue bars) for 3 and 6 days as described in ( A ). Cumulative results are shown and each dot represents a different donor. The error bars in figures denote ± SD. *p

    Journal: Scientific Reports

    Article Title: Developmental induction of human T-cell responses against Candida albicans and Aspergillus fumigatus

    doi: 10.1038/s41598-018-35161-5

    Figure Lengend Snippet: Identification of age-dependent fungi specific multifunctional Th1/Th17 cells. ( A ) CD4 + CD45RA + CD31 + T cells from neonates, infants and children of different age groups, and adults were co-cultured with monocytes pulsed with C . albicans (left panel) or A . fumigatus (right panel). The frequency of T cells expressing intracellular IL-17 (white), IFNγ (black) and both IL-17/IFNγ (grey) were determined by flow cytometry at the indicated time points, analysed by boolean gating and shown as different fractions of cytokine expressing cells in a stacked bar chart. ( B ) Frequency of naïve (CD4 + CD45RA + CD31 + ) or memory (CD4 + CD45RO + ) T cells of adults expressing intracellular IL-17 (white), IFNγ (black) and both IL-17/IFNγ (grey) were determined by flow cytometry at the indicated time points and analysed as described in ( A ). ( C ) Frequency of CD4 + CD45RA + CD31 + T cells from neonates and adults expressing Th1 and Th17 transcription factors T-bet, RORγt and both T-bet as well as RORγt following stimulation with C . albicans (orange bars) or A . fumigatus (blue bars) for 3 and 6 days as described in ( A ). Cumulative results are shown and each dot represents a different donor. The error bars in figures denote ± SD. *p

    Article Snippet: EliSpot assays ELISPOT plates (Millipore 96-well MultiScreen HA; Millipore) were coated with 2 mg/ml anti-human IL-17–specific mAb (eBio64CAP17; eBioscience) in PBS.

    Techniques: Cell Culture, Expressing, Flow Cytometry, Cytometry

    Fungi specific T cells produce IL-17 in an age dependent manner. CD4 + CD45RA + CD31 + T cells from neonates, infants, children, and adults were co-cultured with monocytes pulsed with C . albicans- or A . fumigatus- lysates. ( A , B ) The frequency of T cells expressing signature Th17 molecules IL-17 ( A ) and RORγt ( B ) were analysed by flow cytometry at day 3 (upper Panel) and day 6 after stimulation (lower panel) ( C ) Bar graph representing the ELISPOT analysis of the quantitative IL-17, produced by the T cells from neonates and adults which were either stimulated or not for 3 days as described in ( A ). ( D ) Determination of IL-17A cytokine release of CD4 + CD45RA + T cells of neonates, infants and children or adults by LegendPlex which were either stimulated or not for 3 days as described in ( A ). ( E ) Frequency of T cells expressing intracellular IL-17 (white), IL-4 (black) and both IL-17/IL-4 (grey) were measured by flow cytometry after 6 days of stimulation, analysed by boolean gating and shown as different fractions of cytokine expressing cells in a stacked bar chart. (F) Bar graph showing IL-17 expression by CD4 + CD45RA + CD31 + T cells of neonates, adults, and infants of 0.5-2 years old, stimulated for 6 days as described in ( A ) in the presence or absence of neutralizing antibodies for IL-1ß, IL-6 or both. Cumulative results are shown and each dot in ( A – D ) and ( F ) represent a different donor. The error bars in figures denote ± SD. *p

    Journal: Scientific Reports

    Article Title: Developmental induction of human T-cell responses against Candida albicans and Aspergillus fumigatus

    doi: 10.1038/s41598-018-35161-5

    Figure Lengend Snippet: Fungi specific T cells produce IL-17 in an age dependent manner. CD4 + CD45RA + CD31 + T cells from neonates, infants, children, and adults were co-cultured with monocytes pulsed with C . albicans- or A . fumigatus- lysates. ( A , B ) The frequency of T cells expressing signature Th17 molecules IL-17 ( A ) and RORγt ( B ) were analysed by flow cytometry at day 3 (upper Panel) and day 6 after stimulation (lower panel) ( C ) Bar graph representing the ELISPOT analysis of the quantitative IL-17, produced by the T cells from neonates and adults which were either stimulated or not for 3 days as described in ( A ). ( D ) Determination of IL-17A cytokine release of CD4 + CD45RA + T cells of neonates, infants and children or adults by LegendPlex which were either stimulated or not for 3 days as described in ( A ). ( E ) Frequency of T cells expressing intracellular IL-17 (white), IL-4 (black) and both IL-17/IL-4 (grey) were measured by flow cytometry after 6 days of stimulation, analysed by boolean gating and shown as different fractions of cytokine expressing cells in a stacked bar chart. (F) Bar graph showing IL-17 expression by CD4 + CD45RA + CD31 + T cells of neonates, adults, and infants of 0.5-2 years old, stimulated for 6 days as described in ( A ) in the presence or absence of neutralizing antibodies for IL-1ß, IL-6 or both. Cumulative results are shown and each dot in ( A – D ) and ( F ) represent a different donor. The error bars in figures denote ± SD. *p

    Article Snippet: EliSpot assays ELISPOT plates (Millipore 96-well MultiScreen HA; Millipore) were coated with 2 mg/ml anti-human IL-17–specific mAb (eBio64CAP17; eBioscience) in PBS.

    Techniques: Cell Culture, Expressing, Flow Cytometry, Cytometry, Enzyme-linked Immunospot, Produced

    Regulation of interleukin (IL)-17 production in vitro . IL-17A levels (pg/ml) measured by enzyme-linked immunosorbent assay (ELISA) from supernatants taken from three different culture conditions in healthy individuals. Calculated P values are from two-tailed t -tests between IL-17 levels measured by ELISA from cultures containing anti-CD3, anti-CD3 plus Th17 stimulatory conditions and anti-CD3 plus Th17 stimulatory conditions with the addition of IL-4.

    Journal: Arthritis Research & Therapy

    Article Title: A polymorphism in the interleukin-4 receptor affects the ability of interleukin-4 to regulate Th17 cells: a possible immunoregulatory mechanism for genetic control of the severity of rheumatoid arthritis

    doi: 10.1186/ar3239

    Figure Lengend Snippet: Regulation of interleukin (IL)-17 production in vitro . IL-17A levels (pg/ml) measured by enzyme-linked immunosorbent assay (ELISA) from supernatants taken from three different culture conditions in healthy individuals. Calculated P values are from two-tailed t -tests between IL-17 levels measured by ELISA from cultures containing anti-CD3, anti-CD3 plus Th17 stimulatory conditions and anti-CD3 plus Th17 stimulatory conditions with the addition of IL-4.

    Article Snippet: Intracellular cytokine staining was performed using fluorescein isothiocyanate (FITC)-labeled anti-human IFN-γ (BD Bioscience) and phycoerythrin (PE)-labeled anti-human IL-17A (Ebioscience), or FITC-conjugated mIgG1 isotype control (Ebioscience) and PE-conjugated mouse IgG1 isotype control (Ancell, Bayport, MN, USA).

    Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Two Tailed Test

    Flow cytometric enumeration of Th17 cells following a 5-day culture of peripheral blood mononuclear cells (PBMCs) . (A) Representative flow cytometry histograms showing control PBMCs stained for CD4 and interleukin (IL)-17A after stimulation with anti-CD3, anti-CD3 and Th17 stimulatory conditions and anti-CD3 and Th17 stimulatory conditions with IL4. Numbers in quadrants represent the percentage of total cells expressing IL-17A. (B) Th17 and Th17/Th1 cell numbers generated in RA patient and control cultures. The difference between each cell type was statistically significant, P

    Journal: Arthritis Research & Therapy

    Article Title: A polymorphism in the interleukin-4 receptor affects the ability of interleukin-4 to regulate Th17 cells: a possible immunoregulatory mechanism for genetic control of the severity of rheumatoid arthritis

    doi: 10.1186/ar3239

    Figure Lengend Snippet: Flow cytometric enumeration of Th17 cells following a 5-day culture of peripheral blood mononuclear cells (PBMCs) . (A) Representative flow cytometry histograms showing control PBMCs stained for CD4 and interleukin (IL)-17A after stimulation with anti-CD3, anti-CD3 and Th17 stimulatory conditions and anti-CD3 and Th17 stimulatory conditions with IL4. Numbers in quadrants represent the percentage of total cells expressing IL-17A. (B) Th17 and Th17/Th1 cell numbers generated in RA patient and control cultures. The difference between each cell type was statistically significant, P

    Article Snippet: Intracellular cytokine staining was performed using fluorescein isothiocyanate (FITC)-labeled anti-human IFN-γ (BD Bioscience) and phycoerythrin (PE)-labeled anti-human IL-17A (Ebioscience), or FITC-conjugated mIgG1 isotype control (Ebioscience) and PE-conjugated mouse IgG1 isotype control (Ancell, Bayport, MN, USA).

    Techniques: Flow Cytometry, Cytometry, Staining, Expressing, Generated

    Effects of rHMGB1 on apoptosis and differentiation of Treg and Th17 cells from normal coronary arteries (NCA) group. (A) and (B) Representative flow cytometric dot plots for apoptotic Treg cells (gated by CD4 + CD25 + CD127 - Annexin V + cells) and apoptotic Th17 cells (gated by CD3 + CD8 - IL-17 + Annexin V + cells) with or without 100 ng/mL rHMGB1 stimulation for 24 h in NCA group. (C) A summary of the percentage of apoptotic Treg and Th17 cells with or without 100 ng/mL rHMGB1 stimulation for 24 h in NCA group (n = 8); (D) Representative flow cytometric dot plots for purified naive CD4 + T cells (gated by CD4 + CD45RA + cells) from NCA group; (E) and (F) Representative flow cytometric dot plots for Treg cells (gated by CD4 + CD25 + CD127 - cells) and Th17 cells (gated by CD4 + IL-17 + cells) in blank (without induction), control (induction without rHMGB1 stimulation) and rHMGB1 (induction with 100 ng/mL rHMGB1 sitmulation) group; (G) Sum up Treg and Th17 cells frequencies after induction from naive CD4 + T cells with or without rHMGB1 stimulation (n = 6); (H) A summary of IL-10 and IL-17A levels in supernatants from each culture group (n = 6). (* p

    Journal: Acta Cardiologica Sinica

    Article Title: The Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis

    doi: 10.6515/ACS.201809_34(5).20180419A

    Figure Lengend Snippet: Effects of rHMGB1 on apoptosis and differentiation of Treg and Th17 cells from normal coronary arteries (NCA) group. (A) and (B) Representative flow cytometric dot plots for apoptotic Treg cells (gated by CD4 + CD25 + CD127 - Annexin V + cells) and apoptotic Th17 cells (gated by CD3 + CD8 - IL-17 + Annexin V + cells) with or without 100 ng/mL rHMGB1 stimulation for 24 h in NCA group. (C) A summary of the percentage of apoptotic Treg and Th17 cells with or without 100 ng/mL rHMGB1 stimulation for 24 h in NCA group (n = 8); (D) Representative flow cytometric dot plots for purified naive CD4 + T cells (gated by CD4 + CD45RA + cells) from NCA group; (E) and (F) Representative flow cytometric dot plots for Treg cells (gated by CD4 + CD25 + CD127 - cells) and Th17 cells (gated by CD4 + IL-17 + cells) in blank (without induction), control (induction without rHMGB1 stimulation) and rHMGB1 (induction with 100 ng/mL rHMGB1 sitmulation) group; (G) Sum up Treg and Th17 cells frequencies after induction from naive CD4 + T cells with or without rHMGB1 stimulation (n = 6); (H) A summary of IL-10 and IL-17A levels in supernatants from each culture group (n = 6). (* p

    Article Snippet: The cells were fixed and permeabilized using an Intracellular Fixation & Permeabilization Buffer Set Kit (Lot 85-88-8824-00, eBioscience, America) according to the manufacturer’s instructions and stained for anti-human IL-17A-PE (Lot 12-7178-42, eBioscience, America).

    Techniques: Flow Cytometry, Purification

    Maternal exercise induces DNA demethylation of the Prdm16 promoter through apelin/α-KG axis. ( A ) mRNA expression of Prdm16 of fetal BAT from M-Ctrl and M-Ex ( n = 6). Expression was normalized by Δ C t values. ( B ) Diagram showing three regions in the Prdm16 promoter. ( C , D , and H ) 5mC and 5hmC enrichment fold of fetal BAT (ME study, C; APN study, H) and adult offspring challenged with HFD (D) ( n = 6). ( E ) α-KG to 2-hydroxyglutarate (2-HG) ratio of fetal iBAT from ME or APN study ( n = 6). ( F , G , I , and J ) Z scores of mRNA level of ten-eleven translocation (Tet) and IDH families in the fetal iBAT of APN study (I and J) or ME study (F and G) ( n = 6). Expression was normalized by Δ C t values. ( K ) Working model: DNA demethylation stimulated by α-KG/AMPK/maternal exercise–induced apelin. Data are mean ± SEM, and each dot represents one litter. * P

    Journal: Science Advances

    Article Title: Maternal exercise via exerkine apelin enhances brown adipogenesis and prevents metabolic dysfunction in offspring mice

    doi: 10.1126/sciadv.aaz0359

    Figure Lengend Snippet: Maternal exercise induces DNA demethylation of the Prdm16 promoter through apelin/α-KG axis. ( A ) mRNA expression of Prdm16 of fetal BAT from M-Ctrl and M-Ex ( n = 6). Expression was normalized by Δ C t values. ( B ) Diagram showing three regions in the Prdm16 promoter. ( C , D , and H ) 5mC and 5hmC enrichment fold of fetal BAT (ME study, C; APN study, H) and adult offspring challenged with HFD (D) ( n = 6). ( E ) α-KG to 2-hydroxyglutarate (2-HG) ratio of fetal iBAT from ME or APN study ( n = 6). ( F , G , I , and J ) Z scores of mRNA level of ten-eleven translocation (Tet) and IDH families in the fetal iBAT of APN study (I and J) or ME study (F and G) ( n = 6). Expression was normalized by Δ C t values. ( K ) Working model: DNA demethylation stimulated by α-KG/AMPK/maternal exercise–induced apelin. Data are mean ± SEM, and each dot represents one litter. * P

    Article Snippet: PRDM16 (PA5-20872; Thermo Fisher Scientific, Rockford, IL) and PGC-1α (no. 66369-1-Ig; Proteintech, Rosemont, IL) were also purchased.

    Techniques: Expressing, Translocation Assay

    Maternal exercise intervention markedly induces the expression of thermogenic markers in iBAT and ingWAT of offspring at weaning. ( A and B ) Body weight (A) and relative wet weight of iBAT and ingWAT (B) normalized to body weight of female and male M-Ctrl and M-Ex offspring at weaning ( n = 6 mice per group). N.S., not significant. ( C ) Z scores of mRNA levels of different brown fat markers in the iBAT and ingWAT of M-Ctrl and M-Ex offspring at weaning ( n = 6 mice per group). Expression was normalized by Δ C t values. ( D ) Cropped Western blots of UCP1, PRDM16, and PGC-1α in the iBAT (β-tubulin is the loading control) from the E18.5 fetuses of CON and EX maternal mice ( n = 6 fetuses per group). ( E and F ) Cropped Western blots of UCP1 and PRDM16 (β-tubulin is the loading control) from iBAT (E) and ingWAT (F) isolated from female and male mice of M-Ctrl and M-Ex at weaning ( n = 6 mice per group). Data are mean ± SEM, and each dot represents one litter. * P

    Journal: Science Advances

    Article Title: Maternal exercise via exerkine apelin enhances brown adipogenesis and prevents metabolic dysfunction in offspring mice

    doi: 10.1126/sciadv.aaz0359

    Figure Lengend Snippet: Maternal exercise intervention markedly induces the expression of thermogenic markers in iBAT and ingWAT of offspring at weaning. ( A and B ) Body weight (A) and relative wet weight of iBAT and ingWAT (B) normalized to body weight of female and male M-Ctrl and M-Ex offspring at weaning ( n = 6 mice per group). N.S., not significant. ( C ) Z scores of mRNA levels of different brown fat markers in the iBAT and ingWAT of M-Ctrl and M-Ex offspring at weaning ( n = 6 mice per group). Expression was normalized by Δ C t values. ( D ) Cropped Western blots of UCP1, PRDM16, and PGC-1α in the iBAT (β-tubulin is the loading control) from the E18.5 fetuses of CON and EX maternal mice ( n = 6 fetuses per group). ( E and F ) Cropped Western blots of UCP1 and PRDM16 (β-tubulin is the loading control) from iBAT (E) and ingWAT (F) isolated from female and male mice of M-Ctrl and M-Ex at weaning ( n = 6 mice per group). Data are mean ± SEM, and each dot represents one litter. * P

    Article Snippet: PRDM16 (PA5-20872; Thermo Fisher Scientific, Rockford, IL) and PGC-1α (no. 66369-1-Ig; Proteintech, Rosemont, IL) were also purchased.

    Techniques: Expressing, Mouse Assay, Western Blot, Pyrolysis Gas Chromatography, Isolation

    Maternal exercise intervention facilitates thermogenesis in iBAT and ingWAT of offspring following CE. ( A ) iBAT wet weight of female and male M-Ctrl and M-Ex offspring by 2 days of CE ( n = 5 mice per group). ( B ) Representative thermographic images (left) and calculated averages of intrascapular temperature (right) of female and male M-Ctrl and M-Ex offspring at room temperature (RT) and 2 days of CE ( n = 6 litters for RT and n = 5 litters for CE). ( C and D ) Cropped Western blots of UCP1 and PRDM16 (β-tubulin is the loading control) from iBAT (C) and ingWAT (D) of female and male M-Ctrl and M-Ex offspring followed by 2 days of CE ( n = 5 mice per group). ( E ) Representative images of UCP1 immunocytochemical staining of iBAT and ingWAT from M-Ctrl or M-Ex offspring at RT or CE. Scale bars, 100 μm. DAPI, 4′,6-diamidino-2-phenylindole. ( F ) Cropped Western blots of GLUT4 in the iBAT (β-actin was used as the loading control) isolated from M-Ctrl or M-Ex offspring at RT and after 2 days of CE ( n = 6 litters for RT and n = 5 litters for CE). Data are mean ± SEM, and each dot represents one litter. * P

    Journal: Science Advances

    Article Title: Maternal exercise via exerkine apelin enhances brown adipogenesis and prevents metabolic dysfunction in offspring mice

    doi: 10.1126/sciadv.aaz0359

    Figure Lengend Snippet: Maternal exercise intervention facilitates thermogenesis in iBAT and ingWAT of offspring following CE. ( A ) iBAT wet weight of female and male M-Ctrl and M-Ex offspring by 2 days of CE ( n = 5 mice per group). ( B ) Representative thermographic images (left) and calculated averages of intrascapular temperature (right) of female and male M-Ctrl and M-Ex offspring at room temperature (RT) and 2 days of CE ( n = 6 litters for RT and n = 5 litters for CE). ( C and D ) Cropped Western blots of UCP1 and PRDM16 (β-tubulin is the loading control) from iBAT (C) and ingWAT (D) of female and male M-Ctrl and M-Ex offspring followed by 2 days of CE ( n = 5 mice per group). ( E ) Representative images of UCP1 immunocytochemical staining of iBAT and ingWAT from M-Ctrl or M-Ex offspring at RT or CE. Scale bars, 100 μm. DAPI, 4′,6-diamidino-2-phenylindole. ( F ) Cropped Western blots of GLUT4 in the iBAT (β-actin was used as the loading control) isolated from M-Ctrl or M-Ex offspring at RT and after 2 days of CE ( n = 6 litters for RT and n = 5 litters for CE). Data are mean ± SEM, and each dot represents one litter. * P

    Article Snippet: PRDM16 (PA5-20872; Thermo Fisher Scientific, Rockford, IL) and PGC-1α (no. 66369-1-Ig; Proteintech, Rosemont, IL) were also purchased.

    Techniques: Mouse Assay, Western Blot, Staining, Isolation

    Maternal exercise protects the impairment of thermogenic markers and formation in iBAT and ingWAT of the offspring challenged with HFD. ( A and B ) Relative iBAT wet weight (A) and representative thermographic images (left) and calculated averages of intrascapular temperature (right) (B) of female and male M-Ctrl and M-Ex offspring challenged with HFD ( n = 6). ( C and F ) Cropped Western blots of UCP1 and PRDM16 protein levels in the iBAT (C) and ingWAT (β-tubulin or β-actin were used as the loading control) (F) from the female and male M-Ctrl and M-Ex offspring challenged with HFD ( n = 6). ( D and G ) Hematoxylin and eosin (H E) staining (D) and transmission electron microscopic images (G) of iBAT of female and male offspring challenged with HFD. Scale bars, 100 μm (D) and 1 μm (G); m.: mitochondria; l.: lipid droplets. ( E and H to J ) H E staining (E), mean cross-sectional areas (CSAs) (H), and percent distribution of lipid droplets (I and J) in the ingWAT of female and male offspring challenged with HFD. Scale bar, 100 μm. Data are mean ± SEM, and each dot represents one litter. * P

    Journal: Science Advances

    Article Title: Maternal exercise via exerkine apelin enhances brown adipogenesis and prevents metabolic dysfunction in offspring mice

    doi: 10.1126/sciadv.aaz0359

    Figure Lengend Snippet: Maternal exercise protects the impairment of thermogenic markers and formation in iBAT and ingWAT of the offspring challenged with HFD. ( A and B ) Relative iBAT wet weight (A) and representative thermographic images (left) and calculated averages of intrascapular temperature (right) (B) of female and male M-Ctrl and M-Ex offspring challenged with HFD ( n = 6). ( C and F ) Cropped Western blots of UCP1 and PRDM16 protein levels in the iBAT (C) and ingWAT (β-tubulin or β-actin were used as the loading control) (F) from the female and male M-Ctrl and M-Ex offspring challenged with HFD ( n = 6). ( D and G ) Hematoxylin and eosin (H E) staining (D) and transmission electron microscopic images (G) of iBAT of female and male offspring challenged with HFD. Scale bars, 100 μm (D) and 1 μm (G); m.: mitochondria; l.: lipid droplets. ( E and H to J ) H E staining (E), mean cross-sectional areas (CSAs) (H), and percent distribution of lipid droplets (I and J) in the ingWAT of female and male offspring challenged with HFD. Scale bar, 100 μm. Data are mean ± SEM, and each dot represents one litter. * P

    Article Snippet: PRDM16 (PA5-20872; Thermo Fisher Scientific, Rockford, IL) and PGC-1α (no. 66369-1-Ig; Proteintech, Rosemont, IL) were also purchased.

    Techniques: Western Blot, Staining, Transmission Assay

    Apelin supplementation induces brown adipogenesis in the fetal BAT at E18.5. ( A ) Cropped Western blots of apelin protein levels in the BAT (β-tubulin was used as the loading control) of fetal and offspring mice ( n = 6). apelin (APLN) ( B ) Serum apelin (APLN) concentrations in maternal mice at E18.5 ( n = 6). ( C ) Protocol of apelin supplementation (i.p.). ( D ) Serum apelin concentrations in female and male fetuses of maternal exercise (ME) and APN studies ( n = 6). ( E ) BAT wet weight of female and male fetuses maternally treated with apelin (0.5 μmol/kg per day) or phosphate-buffered saline (PBS) ( n = 6). ( F and G ) Z scores of mRNA level of different brown fat markers in the female (D) and male (E) fetal BAT ( n = 6). Expression was normalized by Δ C t values. ( H ) Cropped Western blots of UCP1, PGC-1α, PRDM16, and AMPK phosphorylation (β-tubulin was used as the loading control) in the female and male fetal BAT maternally treated with apelin or PBS ( n = 6). Data are mean ± SEM, and each dot represents one litter. * P

    Journal: Science Advances

    Article Title: Maternal exercise via exerkine apelin enhances brown adipogenesis and prevents metabolic dysfunction in offspring mice

    doi: 10.1126/sciadv.aaz0359

    Figure Lengend Snippet: Apelin supplementation induces brown adipogenesis in the fetal BAT at E18.5. ( A ) Cropped Western blots of apelin protein levels in the BAT (β-tubulin was used as the loading control) of fetal and offspring mice ( n = 6). apelin (APLN) ( B ) Serum apelin (APLN) concentrations in maternal mice at E18.5 ( n = 6). ( C ) Protocol of apelin supplementation (i.p.). ( D ) Serum apelin concentrations in female and male fetuses of maternal exercise (ME) and APN studies ( n = 6). ( E ) BAT wet weight of female and male fetuses maternally treated with apelin (0.5 μmol/kg per day) or phosphate-buffered saline (PBS) ( n = 6). ( F and G ) Z scores of mRNA level of different brown fat markers in the female (D) and male (E) fetal BAT ( n = 6). Expression was normalized by Δ C t values. ( H ) Cropped Western blots of UCP1, PGC-1α, PRDM16, and AMPK phosphorylation (β-tubulin was used as the loading control) in the female and male fetal BAT maternally treated with apelin or PBS ( n = 6). Data are mean ± SEM, and each dot represents one litter. * P

    Article Snippet: PRDM16 (PA5-20872; Thermo Fisher Scientific, Rockford, IL) and PGC-1α (no. 66369-1-Ig; Proteintech, Rosemont, IL) were also purchased.

    Techniques: Western Blot, Mouse Assay, Expressing, Pyrolysis Gas Chromatography